keyword
https://read.qxmd.com/read/38474072/lumican-a-multifunctional-cell-instructive-biomarker-proteoglycan-has-novel-roles-as-a-marker-of-the-hypercoagulative-state-of-long-covid-disease
#1
REVIEW
Margaret M Smith, James Melrose
This study has reviewed the many roles of lumican as a biomarker of tissue pathology in health and disease. Lumican is a structure regulatory proteoglycan of collagen-rich tissues, with cell instructive properties through interactions with a number of cell surface receptors in tissue repair, thereby regulating cell proliferation, differentiation, inflammation and the innate and humoral immune systems to combat infection. The exponential increase in publications in the last decade dealing with lumican testify to its role as a pleiotropic biomarker regulatory protein...
February 29, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38464213/regulation-of-corneal-stromal-cell-behavior-by-modulating-curvature-using-a-hydraulically-controlled-organ-chip-array
#2
Jungkyu Jay Kim, Minju Kim, Kanghoon Choi, David Krizaj
Curvature is a critical factor in cornea mechanobiology, but its impact on phenotypic alterations and extracellular matrix remodeling of cornea stroma remains unclear. In this work, we investigated how curvature influences the corneal stroma using a hydraulically controlled curvature array chip. The responses of stromal cells to low, medium, and high curvatures were observed by preparing three phenotypes of corneal stromal cells: corneal keratocytes, fibroblasts, and myofibroblasts. Keratocytes exhibited phenotypic alterations in response to curvature changes, notably including a decrease in ALDH3 expression and an increase in α-SMA expression...
February 23, 2024: Research Square
https://read.qxmd.com/read/38444462/integrative-proteomics-and-metabolomics-explore-the-effect-and-mechanism-of-qiyin-granules-on-improving-nonalcoholic-fatty-liver-disease
#3
JOURNAL ARTICLE
Xuehua Yan, Hongbing Liu, Meng Huang, Yujie Zhang, Binfang Zeng
Nonalcoholic fatty liver disease (NAFLD) has emerged as a prominent global health concern, representing a substantial burden within the spectrum of chronic liver diseases. Despite its escalating prevalence, a definitive therapeutic strategy or efficacious pharmacological intervention for NAFLD has yet to receive official approval to date. While Fu Fang Qiyin granules have exhibited efficacy in addressing NAFLD, the intricacies of their underlying mechanism of action remain inadequately elucidated. In this study, we substantiated the ameliorative impact of Qiyin on highfat diet (HFD)induced NAFLD in rat models...
March 15, 2024: Heliyon
https://read.qxmd.com/read/38380793/assessment-of-the-proteome-profile-of-decellularized-human-amniotic-membrane-and-its-biocompatibility-with-umbilical-cord-derived-mesenchymal-stem-cells
#4
JOURNAL ARTICLE
Kainat Ahmed, Haadia Tauseef, Jahan Ara Ainuddin, Muneeza Zafar, Irfan Khan, Asmat Salim, Munazza Raza Mirza, Omair Anwar Mohiuddin
Extracellular matrix-based bio-scaffolds are useful for tissue engineering as they retain the unique structural, mechanical, and physiological microenvironment of the tissue thus facilitating cellular attachment and matrix activities. However, considering its potential, a comprehensive understanding of the protein profile remains elusive. Herein, we evaluate the impact of decellularization on the human amniotic membrane (hAM) based on its proteome profile, physicochemical features, as well as the attachment, viability, and proliferation of umbilical cord-derived mesenchymal stem cells (hUC-MSC)...
February 21, 2024: Journal of Biomedical Materials Research. Part A
https://read.qxmd.com/read/38326640/repaglinide-restrains-hcc-development-and-progression-by-targeting-foxo3-lumican-p53-axis
#5
JOURNAL ARTICLE
Yifei Tan, Yongjie Zhou, Wei Zhang, Zhenru Wu, Qing Xu, Qiong Wu, Jian Yang, Tao Lv, Lvnan Yan, Hong Luo, Yujun Shi, Jiayin Yang
PURPOSE: The recent focus on the roles of N-linked glycoproteins in carcinogenesis across various malignancies has prompted our exploration of aberrantly expressed glycoproteins responsible for HCC progression and potential therapeutic strategy. METHODS: Mass spectrometry was applied to initially identify abnormally expressed glycoproteins in HCC, which was further assessed by immunohistochemistry (IHC) staining. The role of selected glycoprotein on HCC development and underlying mechanism was systematically investigated by colony formation, mouse xenograft, RNA-sequencing and western blot assays, etc...
February 7, 2024: Cellular Oncology (Dordrecht)
https://read.qxmd.com/read/38322840/system-biology-approaches-to-identify-hub-genes-linked-with-ecm-organization-and-inflammatory-signaling-pathways-in-schizophrenia-pathogenesis
#6
JOURNAL ARTICLE
Piplu Bhuiyan, Zhaochu Sun, Md Arif Khan, Md Arju Hossain, Md Habibur Rahman, Yanning Qian
Schizophrenia (SZ) is a chronic and devastating mental illness that affects around 20 million individuals worldwide. Cognitive deficits and structural and functional changes of the brain, abnormalities of brain ECM components, chronic neuroinflammation, and devastating clinical manifestation during SZ are likely etiological factors shown by affected individuals. However, the pathophysiological events associated with multiple regulatory pathways involved in the brain of this complex disorder are still unclear...
February 15, 2024: Heliyon
https://read.qxmd.com/read/38314285/syt7-synaptotagmin-7-promotes-cervical-squamous-cell-carcinoma
#7
JOURNAL ARTICLE
Jinbing Huang, Wensheng Xu, Qiaoqiao Huang, Erling Chen, Junying Chen
Cervical squamous cell carcinoma (CESC) ranks among the primary contributors to global cancer-associated mortality. However, the role mediated by synaptotagmin 7 (SYT7) in CESC remains unclear. Our study employed immunohistochemistry to assess the level of SYT7 expression in the tissue microarray. Furthermore, lentiviral shRNA transduction was utilized to establish SYT7 knockdown cell line models based on HeLa and SiHa cell lines. The functional impacts of silencing SYT7 expression in vitro were evaluated. A subcutaneous xenograft model was employed to examine the tumorigenic potential of cells with or without SYT7...
February 15, 2024: Heliyon
https://read.qxmd.com/read/38292008/proteomic-analysis-of-the-extracellular-matrix-of-human-atherosclerotic-plaques-shows-marked-changes-between-plaque-types
#8
JOURNAL ARTICLE
Lasse G Lorentzen, Karin Yeung, Nikolaj Eldrup, Jonas P Eiberg, Henrik H Sillesen, Michael J Davies
Cardiovascular disease is the leading cause of death, with atherosclerosis the major underlying cause. While often asymptomatic for decades, atherosclerotic plaque destabilization and rupture can arise suddenly and cause acute arterial occlusion or peripheral embolization resulting in myocardial infarction, stroke and lower limb ischaemia. As extracellular matrix (ECM) remodelling is associated with plaque instability, we hypothesized that the ECM composition would differ between plaques. We analyzed atherosclerotic plaques obtained from 21 patients who underwent carotid surgery following recent symptomatic carotid artery stenosis...
February 2024: Matrix biology plus
https://read.qxmd.com/read/38289027/msc-laden-in-situ-forming-hydrogel-for-preventing-corneal-stromal-opacity
#9
JOURNAL ARTICLE
Yinan Liu, Jing Hong
PURPOSE: The aims of this study were to construct a mesenchymal stem cell (MSC)-laden in situ-forming hydrogel and study its effects on preventing corneal stromal opacity. METHODS: The native gellan gum was modified by high temperature and pressure, and the rabbit bone marrow MSCs were encapsulated before adding Ca2+ to initiate cross-linking. The effects of the hydrogel on 3D culture and gene expression of the rabbit bone marrow MSCs were observed in vitro. Then, the MSC-hydrogel was used to repair corneal stromal injury in New Zealand white rabbits within 28 days postoperation...
January 30, 2024: Cornea
https://read.qxmd.com/read/38286983/runx1-npm1-h3k4me3-complex-contributes-to-extracellular-matrix-remodeling-via-enhancing-fosl2-transcriptional-activation-in-glioblastoma
#10
JOURNAL ARTICLE
Xiaoteng Cui, Dawei Huo, Qixue Wang, Yunfei Wang, Xiaomin Liu, Kai Zhao, Yongping You, Junxia Zhang, Chunsheng Kang
Extracellular matrix (ECM) remodeling has been implicated in the tumor malignant progression and immune escape in glioblastoma (GBM). Runt-related transcription factor 1 (RUNX1) is a vital transcriptional factor for promoting tumorigenesis and invasion in mesenchymal subtype of GBM. But the correlation between RUNX1 and ECM genes expression and regulatory mechanism of RUNX1 on ECM genes expression remain poorly understood to date. In this study, by using integral analysis of chromatin immunoprecipitation-sequencing and RNA sequencing, we reported that RUNX1 positively regulated the expression of various ECM-related genes, including Fibronectin 1 (FN1), Collagen type IV alpha 1 chain (COL4A1), and Lumican (LUM), in GBM...
January 29, 2024: Cell Death & Disease
https://read.qxmd.com/read/38158963/-study-of-urinary-markers-of-different-podocytopathies-by-proteomic-analysis
#11
JOURNAL ARTICLE
A A Vinogradov, N V Chebotareva, A E Bugrova, A G Brzhozovskiy, T N Krasnova, K Z Nasibullina, A S Kononikhin, S V Moiseev
BACKGROUND: Focal segmental glomerulosclerosis (FSGS) is a primary podocytopathy characterized by primary podocyte detection and high proteinuria. The search for biomarkers and factors associated with the progression of this disease is an important task nowdays. AIM: To assess the proteomic profile of urine in patients with FSGS and to isolate urinary biomarkers of podocytopathies. MATERIALS AND METHODS: The study included 41 patients diagnosed with chronic glomerulonephritis, 27 men and 14 women...
August 17, 2023: Terapevticheskiĭ Arkhiv
https://read.qxmd.com/read/38124768/prolonged-use-of-temozolomide-leads-to-increased-anxiety-and-decreased-content-of-aggrecan-and-chondroitin-sulfate-in-brain-tissues-of-aged-rats
#12
JOURNAL ARTICLE
Anastasia V Strokotova, Dmitry K Sokolov, Olga P Molodykh, Elena V Koldysheva, Evgenii E Kliver, Victor S Ushakov, Maxim O Politko, Nadezhda V Mikhnevich, Galina M Kazanskaya, Svetlana V Aidagulova, Elvira V Grigorieva
Chemotherapy with temozolomide (TMZ) is an essential part of anticancer therapy used for malignant tumors (mainly melanoma and glioblastoma); however, the long-term effects on patient health and life quality are not fully investigated. Considering that tumors often occur in elderly patients, the present study was conducted on long-term (4 months) treatment of adult Wistar rats (9 months old, n=40) with TMZ and/or dexamethasone (DXM) to investigate potential behavioral impairments or morphological and molecular changes in their brain tissues...
January 2024: Biomedical Reports
https://read.qxmd.com/read/38081351/downregulation-of-b4galt5-attenuates-cardiac-fibrosis-through-lumican-and-akt-gsk-3%C3%AE-%C3%AE-catenin-pathway
#13
JOURNAL ARTICLE
Xutao Zhang, Shengyu Cui, Yuewen Ding, Yuhua Li, Bing Wu, Jixian Gao, Ming Li, Lin Xu, Hao Xia
Virtually all forms of cardiac disease exhibit cardiac fibrosis as a common trait, which ultimately leads to adverse ventricular remodeling and heart failure. To improve the prognosis of heart disease, it is crucial to halt the progression of cardiac fibrosis. Protein function is intricately linked with protein glycosylation, a vital post-translational modification. As a fundamental member of the β1,4-galactosyltransferase gene family (B4GALT), β1,4-galactosyltransferase V (B4GALT5) is associated with various disorders...
December 9, 2023: European Journal of Pharmacology
https://read.qxmd.com/read/38052928/dysregulated-wound-healing-in-the-pathogenesis-of-urogynecologic-mesh-complications
#14
JOURNAL ARTICLE
Amanda M Artsen, Rui Liang, Leslie Meyn, Megan S Bradley, Pamela A Moalli
To test the hypothesis that dysregulated wound healing is associated with Urogynecologic mesh complications, we collected vaginal cell secretions using vaginal swabs after polypropylene mesh implantation in patients with (N = 39) and without (N = 40) complication. A customized multiplex immunoassay measured markers of inflammation (MCP-1, IGFBP-1, IL-2, IL-10, IL-17, PDGF-BB, bFGF, IL-1b, IL-6, IL-12p70, TNF-α), neuroinflammation (IL-1RA, TGF-β, IL-15, IL-18, IL-3, M-CSF), angiogenesis (VEGF), and matrix proteins (fibronectin, tenasin c, thrombospondin-2, lumican) between groups...
December 5, 2023: Scientific Reports
https://read.qxmd.com/read/38042385/investigating-the-efficacy-and-mechanisms-of-jinfu-an-decoction-in-treating-non-small-cell-lung-cancer-using-network-pharmacology-and-in-vitro-and-in-vivo-experiments
#15
JOURNAL ARTICLE
Huiting Peng, Zhongming Huang, Peiqin Li, Zhe Sun, Xuenan Hou, Zeyun Li, Ran Sang, Zehuai Guo, Siqi Wu, Yang Cao
ETHNOPHARMACOLOGICAL RELEVANCE: Jinfu'an Decoction (JFAD) is a traditional Chinese decoction used in lung cancer treatment to improve patient quality of life and survival. Previous research has established that JFAD has a significant therapeutic effect on non-small cell lung cancer (NSCLC), although the underlying molecular mechanisms have not been largely underexplored. AIM OF THE STUDY: We used network pharmacology to identify the putative active ingredients of JFAD and conducted experimental studies to determine the potential molecular mechanism of JFAD in NSCLC treatment...
November 30, 2023: Journal of Ethnopharmacology
https://read.qxmd.com/read/37990292/development-of-a-predictive-model-to-distinguish-prostate-cancer-from-benign-prostatic-hyperplasia-by-integrating-serum-glycoproteomics-and-clinical-variables
#16
JOURNAL ARTICLE
Caterina Gabriele, Federica Aracri, Licia Elvira Prestagiacomo, Maria Antonietta Rota, Stefano Alba, Giuseppe Tradigo, Pietro Hiram Guzzi, Giovanni Cuda, Rocco Damiano, Pierangelo Veltri, Marco Gaspari
BACKGROUND: Prostate Cancer (PCa) represents the second leading cause of cancer-related death in men. Prostate-specific antigen (PSA) serum testing, currently used for PCa screening, lacks the necessary sensitivity and specificity. New non-invasive diagnostic tools able to discriminate tumoral from benign conditions and aggressive (AG-PCa) from indolent forms of PCa (NAG-PCa) are required to avoid unnecessary biopsies. METHODS: In this work, 32 formerly N-glycosylated peptides were quantified by PRM (parallel reaction monitoring) in 163 serum samples (79 from PCa patients and 84 from individuals affected by benign prostatic hyperplasia (BPH)) in two technical replicates...
November 21, 2023: Clinical Proteomics
https://read.qxmd.com/read/37972853/impaired-healing-in-an-incision-wound-in-corneal-stroma-in-a-lumican-null-mouse
#17
JOURNAL ARTICLE
Eimi Suzuki, Takayoshi Sumioka, Shizuya Saika, Masayasu Miyajima, Shingo Yasuda, Hiroki Iwanishi, Yukihisa Takada, Kana Ichikawa, Jhuwala Venkatakrishnan, Chia-Yang Liu, Winston Whei-Yang Kao, Yuka Okada
PURPOSE: We investigated healing pattern of an incisional wound in corneal stroma of lumican-null (KO) mice. METHODS: C57BL/6 mice (wild-type, WT) and lumican-null (knockout, KO) mice were used. A linear full-thickness incision was produced in one cornea of each mouse. After intervals of healing, the corneas were processed for the following analyses. Histology was employed to measure the distance between each edge of the disrupted Descemet's membrane at the center of the cornea...
November 14, 2023: Ocular Surface
https://read.qxmd.com/read/37960230/intermittent-fasting-attenuates-metabolic-dysfunction-associated-steatohepatitis-by-enhancing-the-hepatic-autophagy-lysosome-pathway
#18
JOURNAL ARTICLE
Kyung Eun Kim, Hyun Joo Shin, Yeajin Ju, Youngae Jung, Hyeong Seok An, So Jeong Lee, Eun Ae Jeong, Jaewoong Lee, Geum-Sook Hwang, Gu Seob Roh
An intermittent fasting (IF) regimen has been shown to protect against metabolic dysfunction-associated steatohepatitis (MASH). However, the precise mechanism remains unclear. Here, we explored how IF reduced hepatic lipid accumulation, inflammation, and fibrosis in mice with MASH. The mice were fed a high-fat diet (HFD) for 30 weeks and either continued on the HFD or were subjected to IF for the final 22 weeks. IF reduced body weight, insulin resistance, and hepatic lipid accumulation in HFD-fed mice. Lipidome analysis revealed that IF modified HFD-induced hepatic lipid composition...
October 27, 2023: Nutrients
https://read.qxmd.com/read/37956822/circulating-lumican-as-a-potential-biomarker-for-osteosarcopenia-in-older-adults
#19
JOURNAL ARTICLE
So Jeong Park, Eunhye Ji, Hyun Ju Yoo, Kyunggon Kim, Sunghwan Ji, Ji Yeon Baek, Jin Young Lee, Hee-Won Jung, Il-Young Jang, Eunju Lee, Namki Hong, Beom-Jun Kim
In vitro and animal experiments demonstrated that lumican exerts anabolic effects on bone and muscle by stimulating osteoblastogenesis, suppressing osteoclastogenesis and increasing myogenesis. However, the relationship between circulating lumican and musculoskeletal phenotypes in humans remains unclear. We aimed to analyze the relationship between serum lumican levels and osteosarcopenia in older adults. Blood samples were collected from 134 participants (age: 65 years and older) who underwent comprehensive assessment of bone and muscle phenotypes...
November 11, 2023: Bone
https://read.qxmd.com/read/37947602/microrna-and-protein-cargos-of-human-limbal-epithelial-cell-derived-exosomes-and-their-regulatory-roles-in-limbal-stromal-cells-of-diabetic-and-non-diabetic-corneas
#20
JOURNAL ARTICLE
Nagendra Verma, Drirh Khare, Adam J Poe, Cynthia Amador, Sean Ghiam, Andrew Fealy, Shaghaiegh Ebrahimi, Odelia Shadrokh, Xue-Ying Song, Chintda Santiskulvong, Mitra Mastali, Sarah Parker, Aleksandr Stotland, Jennifer E Van Eyk, Alexander V Ljubimov, Mehrnoosh Saghizadeh
Epithelial and stromal/mesenchymal limbal stem cells contribute to corneal homeostasis and cell renewal. Extracellular vesicles (EVs), including exosomes (Exos), can be paracrine mediators of intercellular communication. Previously, we described cargos and regulatory roles of limbal stromal cell (LSC)-derived Exos in non-diabetic (N) and diabetic (DM) limbal epithelial cells (LECs). Presently, we quantify the miRNA and proteome profiles of human LEC-derived Exos and their regulatory roles in N- and DM-LSC. We revealed some miRNA and protein differences in DM vs...
October 25, 2023: Cells
keyword
keyword
2172
1
2
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.