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https://www.readbyqxmd.com/read/29750298/role-of-smad3-signaling-in-the-epithelial%C3%A2-mesenchymal-transition-of-the-lens-epithelium-following-injury
#1
Fanlan Meng, Jun Li, Xiao Yang, Xiaoyong Yuan, Xin Tang
Transforming growth factor‑β (TGF‑β) is important in the development of posterior capsule opacification (PCO), and inhibition of the TGF‑β pathway may represent a novel method of treating PCO. Drosophila protein, mothers against decapentaplegic homolog 3 (Smad3) is a phosphorylated receptor‑activated Smad required for the transmission of TGF‑β signals. Smad3 knockout (KO) disturbs the activation of TGF‑β signaling, thus inhibiting the onset of PCO. In the present study, lens epithelial cell (LEC) damage induced by extracapsular cataract extraction was simulated by puncture of the anterior capsule using a 26‑gauge hypodermic needle...
May 9, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29678203/quantitative-shotgun-proteomics-distinguishes-wound-healing-biomarker-signatures-in-common-carp-skin-mucus-in-response-to-ichthyophthirius-multifiliis
#2
Mona Saleh, Gokhlesh Kumar, Abdel-Azeem Abdel-Baki, Mohamed A Dkhil, Mansour El-Matbouli, Saleh Al-Quraishy
Ichthyophthirius multifiliis is a ciliated protozoan parasite recognized as one of the most pathogenic diseases of wild and cultured freshwater fish. Fish skin mucus plays a significant role against invading pathogens. However, the protein-based modulation against infection with I. multifiliis, of host fish at this barrier is unknown. Thus, we investigated the skin mucus proteome of common carp using a shotgun proteomic approach at days 1 and 9 after I. multifiliis exposure. We identified 25 differentially expressed proteins in infected carp skin mucus...
April 20, 2018: Veterinary Research
https://www.readbyqxmd.com/read/29653143/phenotypic-characterization-of-the-sirc-statens-seruminstitut-rabbit-cornea-cell-line-reveals-a-mixed-epithelial-and-fibroblastic-nature
#3
Melania Olivieri, Martina Cristaldi, Salvatore Pezzino, Dario Rusciano, Barbara Tomasello, Carmelina Daniela Anfuso, Gabriella Lupo
The aim of the present study was to investigate, in the Statens Seruminstitut Rabbit Cornea (SIRC) cell line, the presence of epithelial and fibroblastic markers, comparing their levels with those of the human Retinal Pigmented Epithelial (ARPE-19) cell line, and the Human Keratocyte (HK) cell line, respectively. SIRC cells, often described as of epithelial origin, are used as a corneal epithelial barrier model to study the permeability of ophthalmic drugs. However, they show a morphology that is more consistent with a fibroblastic cell phenotype, similar to corneal keratocytes...
April 10, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29625490/keratan-sulfate-phenotype-in-the-%C3%AE-1-3-n-acetylglucosaminyltransferase-7-null-mouse-cornea
#4
Stacy L Littlechild, Robert D Young, Bruce Caterson, Hideyuki Yoshida, Maya Yamazaki, Kenji Sakimura, Andrew J Quantock, Tomoya O Akama
Purpose: Synthesis of keratan sulfate (KS) relies on coordinated action of multiple enzymes, including the N-acetylglucosamine-transferring enzyme, β-1,3-N-acetylglucosaminyltransferase-7 (β3GnT7). A mouse model deficient in β3GnT7 was developed to explore structural changes in KS and the extracellular matrix (ECM; i.e., the corneal stroma), elucidate the KS biosynthesis mechanism, and understand its role in corneal organization. Methods: A knockout vector for the β3GnT7-encoding gene, B3gnt7, was created to develop heterozygous- (htz) and homozygous-null (null) knockouts...
March 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29581064/molecular-signatures-of-medullary-thyroid-carcinoma-by-matrix-assisted-laser-desorption-ionisation-mass-spectrometry-imaging
#5
Andrew Smith, Manuel Galli, Isabella Piga, Vanna Denti, Martina Stella, Clizia Chinello, Nicola Fusco, Davide Leni, Marco Manzoni, Gaia Roversi, Mattia Garancini, Angela Ida Pincelli, Vincenzo Cimino, Giulia Capitoli, Fulvio Magni, Fabio Pagni
The main aim of the study was to assess the feasibility of matrix-assisted laser desorption/ionisation mass spectrometry imaging (MALDI-MSI) in the pathological investigation of Medullary Thyroid Carcinoma (MTC). Formalin-fixed paraffin-embedded (FFPE) samples from seven MTC patients were analysed by MALDI-MSI in order to detect proteomic alterations within tumour lesions and to define the molecular profiles of specific findings, such as amyloid deposition and C cell hyperplasia (CCH). nLC-ESI MS/MS was employed for the identification of amyloid components and to select alternative proteomic markers of MTC pathogenesis...
March 24, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/29567548/analysis-of-proteoglycan-expression-in-human-dental-pulp
#6
Ana Carla Raphaelli Nahás-Scocate, Gabrielle Ferrante Alves de Moraes, Helena Bonciani Nader, Carolina Meloni Vicente, Leny Toma
Proteoglycans are glycosylated proteins which have covalently attached highly anionic glycosaminoglycans. They can be located on the extracellular matrix, cell membrane or intracellular granules. To date, few studies have reported the presence of proteoglycans in human dental pulp. OBJECTIVE: The aim of this study was, therefore, to analyze the expression of lumican, versican and glypican proteoglycans in deciduous and permanent human dental pulp by real-time polymerase chain reaction (q-PCR) and immunofluorescence...
March 10, 2018: Archives of Oral Biology
https://www.readbyqxmd.com/read/29567388/inducing-hair-follicle-neogenesis-with-secreted-proteins-enriched-in-embryonic-skin
#7
Sabrina Mai-Yi Fan, Chia-Feng Tsai, Chien-Mei Yen, Miao-Hsia Lin, Wei-Hung Wang, Chih-Chieh Chan, Chih-Lung Chen, Kyle K L Phua, Szu-Hua Pan, Maksim V Plikus, Sung-Liang Yu, Yu-Ju Chen, Sung-Jan Lin
Organ development is a sophisticated process of self-organization. However, despite growing understanding of the developmental mechanisms, little is known about how to reactivate them postnatally for regeneration. We found that treatment of adult non-hair fibroblasts with cell-free extract from embryonic skin conferred upon them the competency to regenerate hair follicles. Proteomics analysis identified three secreted proteins enriched in the embryonic skin, apolipoprotein-A1, galectin-1 and lumican that together were essential and sufficient to induce new hair follicles...
March 13, 2018: Biomaterials
https://www.readbyqxmd.com/read/29549325/downregulation-of-lumican-accelerates-lung-cancer-cell-invasion-through-p120-catenin
#8
Cheng-Ta Yang, Jhy-Ming Li, Wing-Keung Chu, Shu-Er Chow
The overexpression of lumican has been found in lung cancer cells; however, the functional role of lumican in lung cancer cells remains unclear. In this study, we found lumican functioned as a tubulin-binding protein and the depletion of lumican by transfection with its specific shRNA increased lung cancer cell invasion. Such alterations led to morphological changes and actin cytoskeleton remodeling, including the induction of membrane ruffling or protrusion and stress fiber formation, correlated with the increased activities of Rac and Rho...
March 16, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29547593/interleukin-6-promotes-proliferation-but-inhibits-tenogenic-differentiation-via-the-janus-kinase-signal-transducers-and-activators-of-transcription-3-jak-stat3-pathway-in-tendon-derived-stem-cells
#9
Siwei Chen, Ganming Deng, Kaiqun Li, Haonan Zheng, Gang Wang, Bin Yu, Kairui Zhang
BACKGROUND Previous studies demonstrated that tendon-derived stem cells (TDSCs) were vital healing cells and that mRNA expression of anti-inflammatory cytokine IL-6 was significantly upregulated in injured tendons. The aim of the present study was to investigate the effects of IL-6 on the TDSCs in vitro. MATERIAL AND METHODS TDSCs isolated from the Achilles tendons in SD rats were co-cultured with various concentrations of IL-6. Cell proliferation, cell cycle analysis, quantitative real-time PCR, western blotting analysis, and statistical analysis were used in the study...
March 16, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29536619/postnatal-periodontal-ligament-as-a-novel-adult-stem-cell-source-for-regenerative-corneal-cell-therapy
#10
Gary Hin-Fai Yam, Ericia Pei-Wen Teo, Melina Setiawan, Matthew J Lovatt, Nur Zahirah Binte M Yusoff, Matthias Fuest, Bee-Tin Goh, Jodhbir S Mehta
Corneal opacities are a leading cause of global blindness. They are conventionally treated by the transplantation of donor corneal tissue, which is, restricted by a worldwide donor material shortage and allograft rejection. Autologous adult stem cells with a potential to differentiate into corneal stromal keratocytes (CSKs) could offer a suitable choice of cells for regenerative cell therapy. Postnatal periodontal ligament (PDL) contains a population of adult stem cells, which has a similar embryological origin as CSK, that is cranial neural crest...
March 13, 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29501701/lumican-as-a-multivalent-effector-in-wound-healing
#11
Konstantina Karamanou, Gwenn Perrot, Francois-Xavier Maquart, Stéphane Brézillon
Wound healing, a complex physiological process, is responsible for tissue repair after exposure to destructive stimuli, without resulting in complete functional regeneration. Injuries can be stromal or epithelial, and most cases of wound repair have been studied in the skin and cornea. Lumican, a small leucine-rich proteoglycan, is expressed in the extracellular matrices of several tissues, such as the cornea, cartilage, and skin. This molecule has been shown to regulate collagen fibrillogenesis, keratinocyte phenotypes, and corneal transparency modulation...
March 1, 2018: Advanced Drug Delivery Reviews
https://www.readbyqxmd.com/read/29499356/a-role-for-proteoglycans-in-vascular-disease
#12
REVIEW
Thomas N Wight
The content of proteoglycans (PGs) is low in the extracellular matrix (ECM) of vascular tissue, but increases dramatically in all phases of vascular disease. Early studies demonstrated that glycosaminoglycans (GAGs) including chondroitin sulfate (CS), dermatan sulfate (DS), keratan sulfate (KS) and heparan sulfate (HS) accumulate in vascular lesions in both humans and in animal models in areas of the vasculature that are susceptible to disease initiation (such as at branch points) and are frequently coincident with lipid deposits...
February 27, 2018: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/29427759/quantitative-analysis-of-core-fucosylation-of-serum-proteins-in-liver-diseases-by-lc-ms-mrm
#13
Junfeng Ma, Miloslav Sanda, Renhuizi Wei, Lihua Zhang, Radoslav Goldman
Aberrant core fucosylation of proteins has been linked to liver diseases. In this study, we carried out multiple reaction monitoring (MRM) quantification of core fucosylated N-glycopeptides of serum proteins partially deglycosylated by a combination of endoglycosidases (endoF1, endoF2, and endoF3). To minimize variability associated with the preparatory steps, the analysis was performed without enrichment of glycopeptides or fractionation of serum besides the nanoRP chromatography. Specifically, we quantified core fucosylation of 22 N-glycopeptides derived from 17 proteins together with protein abundance of these glycoproteins in a cohort of 45 participants (15 disease-free control, 15 fibrosis and 15 cirrhosis patients) using a multiplex nanoUPLC-MS-MRM workflow...
February 7, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/29340639/biomechanical-rigidity-and-quantitative-proteomics-analysis-of-segmental-regions-of-the-trabecular-meshwork-at-physiologic-and-elevated-pressures
#14
Janice A Vranka, Julia A Staverosky, Ashok P Reddy, Phillip A Wilmarth, Larry L David, Ted S Acott, Paul Russell, Vijay Krishna Raghunathan
Purpose: The extracellular matrix (ECM) of the trabecular meshwork (TM) modulates resistance to aqueous humor outflow, thereby regulating IOP. Glaucoma, a leading cause of irreversible blindness worldwide, is associated with changes in the ECM of the TM. The elastic modulus of glaucomatous TM is larger than age-matched normal TM; however, the biomechanical properties of segmental low (LF) and high flow (HF) TM regions and their response to elevated pressure, are unknown. Methods: We perfused human anterior segments at two pressures using an ex vivo organ culture system...
January 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29338928/immunoproteomic-identification-of-antigenic-candidate-campylobacter-jejuni-and-human-peripheral-nerve-proteins-involved-in-guillain-barr%C3%A3-syndrome
#15
Aida Loshaj-Shala, Mara Colzani, Katerina Brezovska, Ana Poceva Panovska, Ljubica Suturkova, Giangiacomo Beretta
Immunoproteomics is become a potent methodology used for identifying immunoreactive proteins. In this study, an immunoproteomic approach based on 2-dimensional gel electrophoresis (2D-PAGE) and immunoblotting combined with high resolution mass spectrometry (MS) was used to identify immunoreactive proteins that might be involved in mechanisms of Guillain-Barré syndrome (GBS) development, regardless of their potential reciprocal molecular mimicry. Proteins isolated from C. jejuni and human peripheral nerve tissue (HPN) were separated with 2D SDS-PAGE and subjected to western blotting using serum samples from GBS patients...
April 15, 2018: Journal of Neuroimmunology
https://www.readbyqxmd.com/read/29323130/in-vivo-effect-of-opticin-deficiency-in-cartilage-in-a-surgically-induced-mouse-model-of-osteoarthritis
#16
Aina Farrán, Gladys Valverde-Franco, Laura Tío, Bertrand Lussier, Hassan Fahmi, Jean-Pierre Pelletier, Paul N Bishop, Jordi Monfort, Johanne Martel-Pelletier
The SLRP opticin (OPTC) has been demonstrated to be produced and degraded in osteoarthritic (OA) human cartilage. Here, we investigated the in vivo effect of OPTC deficiency in OA cartilage. OA was induced in 10-week-old Optc -/- and Optc +/+ mice. Ten weeks post-surgery, cartilage was processed for histology and immunohistochemistry. SLRP expression was determined in non-operated mouse cartilage. OA Optc -/- demonstrated significant protection against cartilage degradation. Data revealed that in non-operated Optc -/- cartilage, expression of SLRPs lumican and epiphycan was up-regulated at day 3 and in 10-week-olds (p ≤ 0...
January 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29177105/investigating-molecular-connections-of-non-alcoholic-fatty-liver-disease-with-associated-pathological-conditions-in-west-virginia-for-biomarker-analysis
#17
Dana L Sharma, Hari Vishal Lakhani, Rebecca L Klug, Brian Snoad, Rawan El-Hamdani, Joseph I Shapiro, Komal Sodhi
Non-alcoholic fatty liver disease (NAFLD) is a disease characterized by a steatosis of the liver that may progress to more serious pathological conditions including: nonalcoholic steatohepatitis (NASH), fibrosis, and cirrhosis. As the prevalence of NAFLD has increased worldwide in recent years, pathophysiology and risk factors associated with disease progression of NAFLD are at the focus of many studies. NAFLD is related to and shares common serum biomarkers with cardiovascular disease (CVD), type 2 diabetes mellitus (T2DM), obesity, and metabolic syndrome (MetS)...
October 2017: Journal of Clinical & Cellular Immunology
https://www.readbyqxmd.com/read/29132420/substance-p-and-patterned-silk-biomaterial-stimulate-periodontal-ligament-stem-cells-to-form-corneal-stroma-in-a-bioengineered-three-dimensional-model
#18
Jialin Chen, Wei Zhang, Peyman Kelk, Ludvig J Backman, Patrik Danielson
BACKGROUND: We aimed to generate a bioengineered multi-lamellar human corneal stroma tissue in vitro by differentiating periodontal ligament stem cells (PDLSCs) towards keratocytes on an aligned silk membrane. METHODS: Human PDLSCs were isolated and identified. The neuropeptide substance P (SP) was added in keratocyte differentiation medium (KDM) to evaluate its effect on keratocyte differentiation of PDLSCs. PDLSCs were then seeded on patterned silk membrane and cultured with KDM and SP...
November 13, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29129081/identification-of-mature-atherosclerotic-plaque-proteome-signatures-using-data-independent-acquisition-mass-spectrometry
#19
Nicole Hansmeier, Josef Buttigieg, Pankaj Kumar, Shaneen Pelle, Kyoo Yoon Choi, David Kopriva, Tzu-Chiao Chao
Atherosclerosis is a chronic inflammatory disease with complex pathobiology and one of the most common causes of cardiovascular events. The process is characterized by complex vascular remodeling processes that require the actions of numerous proteins. The composition of atherosclerotic plaque is increasingly recognized as a major factor governing the occurrence of cardiovascular or neurological symptoms. To gain deeper insights into the composition of atherosclerotic plaques, we created quantitative proteome profiles of advanced plaque tissues of six male patients undergoing carotid endarterectomy for stroke prevention...
January 5, 2018: Journal of Proteome Research
https://www.readbyqxmd.com/read/29121102/histopathologic-and-immunohistochemical-features-of-capsular-tissue-around-failed-ahmed-glaucoma-valves
#20
Alka Mahale, Fatma Fikri, Khitam Al Hati, Sami Al Shahwan, Ibrahim Al Jadaan, Hind Al Katan, Rajiv Khandekar, Azza Maktabi, Deepak P Edward
Impervious encapsulation around Ahmed glaucoma valve (AGV) results in surgical failure raising intraocular pressure (IOP). Dysregulation of extracellular matrix (ECM) molecules and cellular factors might contribute to increased hydraulic resistance to aqueous drainage. Therefore, we examined these molecules in failed AGV capsular tissue. Immunostaining for ECM molecules (collagen I, collagen III, decorin, lumican, chondroitin sulfate, aggrecan and keratan sulfate) and cellular factors (αSMA and TGFβ) was performed on excised capsules from failed AGVs and control tenon's tissue...
2017: PloS One
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