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Lumican

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https://www.readbyqxmd.com/read/28059449/irx3-and-bmp2-regulate-mouse-mesenchymal-cell-chondrogenic-differentiation-in-both-a-sox9-dependent-and-independent-manner
#1
Yoshihiro Tamamura, Kenichi Katsube, Hisashi Mera, Maki Itokazu, Shigeyuki Wakitani
Sox9, a master regulator of cartilage development, controls the cell fate decision to differentiate from mesenchymal to chondrogenic cells. In addition, Sox9 regulates the proliferation and differentiation of chondrocytes, as well as the production of cartilage-specific proteoglycans. The existence of Sox9-independent mechanisms in cartilage development remains to be determined. Here, we attempted to identify genes involved in such putative mechanisms via microarray analysis using a mouse chondrogenic cell line, N1511...
January 6, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28054366/the-role-of-molecular-pain-biomarkers-in-temporomandibular-joint-internal-derangement
#2
REVIEW
Malin Ernberg
There is evidence that low-grade inflammation may be responsible for pain and development of degenerative changes in temporomandibular joint internal derangement. This article reviews the current knowledge of the molecular mechanisms behind TMJ internal derangements. A non-systematic search was carried out in PubMed, Embase, and the Cochrane library for studies regarding pathophysiological mechanisms behind internal derangements focusing on pain mediating inflammatory and cartilage degrading molecules. Recent data suggests that release of cytokines may be the key event for pain and cartilage destruction in TMJ internal derangements...
January 5, 2017: Journal of Oral Rehabilitation
https://www.readbyqxmd.com/read/28008374/comparative-proteomics-analysis-of-human-osteosarcoma-by-2d-dige-with-maldi-tof-tof-ms
#3
Guoxiang Wang, Zhengyao Zhang, Maoguang Yang, Bo Xu, Qi Gao, Xiaoyu Yang
Osteosarcoma (OS) is the most common primary malignant tumor of bone and the third most common cancer in childhood and adolescence. However, controversy concerning the ideal combination of chemotherapy agents ensued throughout the last quarter of the 20th century because of conflicting and often nonrandomized data. Collaborative efforts to increase understanding of the biology of osteosarcoma and the use of preclinical models to test novel protein targets will be critical to identify the path toward improving outcomes for patients...
November 2016: Journal of Bone Oncology
https://www.readbyqxmd.com/read/27939890/lumican-null-mice-are-susceptible-to-aging-and-isoproterenol-induced-myocardial-fibrosis
#4
Shao-Wei Chen, Ying-Chang Tung, Shih-Ming Jung, Yen Chu, Pyng-Jing Lin, Winston W-Y Kao, Pao-Hsien Chu
With aging and stress, the myocardium undergoes structural remodeling, often leading to fibrosis. The purpose of this study is to examine whether lumican, one of the class II small leucine-rich proteoglycans, has a protective role in cardiac remodeling and fibrosis. In attempts to elucidate the hypothesis that lumican may have a protective role in cardiac remodeling and fibrosis, we compared the cardiac phenotypes of young (3-month-old) and elder (6-month- and 12-month-old) lumican-null (Lum(-/-)) mice. Extra-cellular matrix remodeling and apoptosis are examined to determine the roles of lumican on age-dependent cardiac fibrosis induced by isoproterenol...
January 22, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27852402/-changes-of-ocular-biological-parameters-and-lumican-expression-in-the-monocularly-deprivation-myopic-model-of-mutant-lumican-transgenic-mice
#5
M S Sun, Y Z Song, F J Zhang, J Tao, Y B Liu
Objective: To investigate ocular changes in the monocularly deprivation myopic model of mutant Lumican transgenic mice. Comparing influences on biological parameters and sclera development between Lumican transgenic and form deprivation mice, and to prepare for further study of pathogenesis of pathological myopia (PM). Methods: Experimental research. Lumican transgenic mice and wild mice were monocularly lid-sutured at ten days after birth. All eyes were divided into 6 groups, group A(32 eyes): control eyes in transgenic mice; group B(34 eyes): sutured eyes in transgenic mice; group C(34 eyes): fellow eyes in transgenic mice; group D(28 eyes): control eyes in wild mice; group E(32 eyes): sutured eyes in wild mice; group F(32 eyes): fellow eyes in wild mice...
November 11, 2016: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
https://www.readbyqxmd.com/read/27826181/analysis-of-the-association-between-the-lum-rs3759223-variant-and-high-myopia-in-a-japanese-population
#6
Shintaro Okui, Akira Meguro, Masaki Takeuchi, Takahiro Yamane, Eiichi Okada, Yasuhito Iijima, Nobuhisa Mizuki
PURPOSE: Many studies have investigated the relationship of the lumican gene (LUM) rs3759223 variant with the risk of high myopia, but the results have been inconsistent and inconclusive. In this study, we investigated whether LUM rs3759223 is associated with high myopia in a Japanese population. METHODS: We recruited 1,585 Japanese patients with high myopia (spherical equivalent [SE] <-9.00 diopters [D]) and 1,011 Japanese healthy controls (SE ≥-1.00 D). The rs3759223 variant was genotyped using the TaqMan assay, and the allelic and genotypic diversity among cases and controls was analyzed according to the SE level...
2016: Clinical Ophthalmology
https://www.readbyqxmd.com/read/27769536/identification-of-the-most-abundant-proteins-in-equine-amniotic-fluid-by-a-proteomic-approach
#7
Gloria Isani, Enea Ferlizza, Aurora Cuoghi, Elisa Bellei, Emanuela Monari, Barbara Bianchin Butina, Carolina Castagnetti
Characterisation of the physiologic equine amniotic fluid (AF) proteome is a prerequisite to study its changes during diseases and discover new biomarkers. The aim of this study was to identify by a proteomic approach the most abundant proteins of equine AF. AF samples were collected at parturition from 24 healthy mares that delivered healthy foals. All samples were subjected to sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) on 4-12% gels. A pool of the 24 samples, after SDS-PAGE, was cut in 25 slices, trypsin-digested and analysed by mass spectrometry (MS) for protein identification...
November 2016: Animal Reproduction Science
https://www.readbyqxmd.com/read/27711221/enlargement-of-the-axial-length-and-altered-ultrastructural-features-of-the-sclera-in-a-mutant-lumican-transgenic-mouse-model
#8
Yanzheng Song, Fengju Zhang, Yanyan Zhao, Mingshen Sun, Jun Tao, Yanchuang Liang, Ling Ma, Yanqiu Yu, Jianhua Wang, Junfeng Hao
Lumican (LUM) is a candidate gene for myopia in the MYP3 locus. In this study, a mutant lumican (L199P) transgenic mouse model was established to investigate the axial length changes and ultrastructural features of the sclera. The mouse model was established by pronuclear microinjection. Transgenic mice and wild-type B6 mice were killed at eight weeks of age. Gene expression levels of LUM and collagen type I (COL1) in the sclera were analyzed by quantitative real-time polymerase chain reaction (qPCR), and the protein levels were assessed by Western blot analysis...
2016: PloS One
https://www.readbyqxmd.com/read/27706836/identifying-nonalcoholic-fatty-liver-disease-patients-with-active-fibrosis-by-measuring-extracellular-matrix-remodeling-rates-in-tissue-and-blood
#9
Martin L Decaris, Kelvin W Li, Claire L Emson, Michelle Gatmaitan, Shanshan Liu, Yenny Wang, Edna Nyangau, Marc Colangelo, Thomas E Angel, Carine Beysen, Jeffrey Cui, Carolyn Hernandez, Len Lazaro, David A Brenner, Scott M Turner, Marc K Hellerstein, Rohit Loomba
: Excess collagen synthesis (fibrogenesis) in the liver plays a causal role in the progression of nonalcoholic fatty liver disease (NAFLD). Methods are needed to identify patients with more rapidly progressing disease and to demonstrate early response to treatment. We describe here a novel method to quantify hepatic fibrogenesis flux rates both directly in liver tissue and noninvasively in blood. Twenty-one patients with suspected NAFLD ingested heavy water ((2) H2 O, 50-mL aliquots) two to three times daily for 3-5 weeks prior to a clinically indicated liver biopsy...
January 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/27693693/lumican-alleviates-hypertrophic-scarring-by-suppressing-integrin-fak-signaling
#10
Yuqian Zhao, Xueyong Li, Xiaoli Xu, Zhi He, Lei Cui, Xiaoxing Lv
Hypertrophic scarring (HS) is an overcompensation of wound healing that increases the risk of cosmetic disfigurement and functional impairment. No gold standard has been established for the treatment or prevention of HS. Our study aims to elucidate the expression and function of lumican in the pathogenesis of HS as well as the underlying mechanism involved in this procedure. An animal model of HS (rabbit ear) was established, and the Ad-lumican vectors were locally injected. Primary fibroblasts isolated from patients with hypertrophic burn scars were used in vitro...
September 29, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27685949/corneal-keratocyte-transition-to-mesenchymal-stem-cell-phenotype-and-reversal-using-serum-free-medium-supplemented-with-fgf-2-tgf-%C3%A3-3-and-retinoic-acid
#11
Laura E Sidney, Andrew Hopkinson
Keratocytes of the corneal limbal stroma can derive populations of mesenchymal stem cells (MSC) when expanded in vitro. However, once a corneal MSC (cMSC) phenotype is achieved, regaining the keratocyte phenotype can be challenging, and there is no standardised differentiation medium. Here, we investigated the transition of keratocytes to cMSC and compared different supplements in their ability to return cMSC to a keratocyte phenotype. Immunofluorescence and RT-qPCR demonstrated in vivo keratocyte expression of ALDH3A1, CD34 and keratocan, but not any of the typical MSC markers (CD73, CD90, CD105)...
September 29, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27682997/lumican-negatively-controls-the-pathogenicity-of-murine-encephalitic-th17-cells
#12
Eliseo F Castillo, Handong Zheng, Christian Van Cabanlong, Fei Dong, Yan Luo, Yi Yang, Meilian Liu, Winston W-Y Kao, Xuexian O Yang
TH17 cells play an essential role in the development of both human multiple sclerosis and animal experimental autoimmune encephalomyelitis (EAE). Nevertheless, it is not well understood how the pathogenicity of TH17 cells is controlled in the autoimmune neuroinflammation. In vitro, we found Lumican (Lum), an extracellular matrix (ECM) protein, is selectively expressed by TH17 cells among tested murine TH subsets. Lum deficiency leads to earlier onset and enhanced severity of EAE. This enhanced disease in Lum-deficient mice is associated with increased production of IL-17 and IL-21 and decreased TH17 cell apoptosis...
December 2016: European Journal of Immunology
https://www.readbyqxmd.com/read/27672590/construction-of-adenovirus-vectors-encoding-the-lumican-gene-by-gateway-recombinant-cloning-technology
#13
Gui-Fang Wang, Bing Qi, Lei-Lei Tu, Lian Liu, Guo-Cheng Yu, Jing-Xiang Zhong
AIM: To construct adenovirus vectors of lumican gene by gateway recombinant cloning technology to further understand the role of lumican gene in myopia. METHODS: Gateway recombinant cloning technology was used to construct adenovirus vectors. The wild-type (wt) and mutant (mut) forms of the lumican gene were synthesized and amplified by polymerase chain reaction (PCR). The lumican cDNA fragments were purified and ligated into the adenovirus shuttle vector pDown-multiple cloning site (MCS)-/internal ribozyme entry site (IRES)/enhanced green fluorescent protein (EGFP)...
2016: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/27631350/cell-therapy-of-corneal-diseases
#14
Winston W-Y Kao, Vivien J Coulson-Thomas
Mesenchymal stem cells isolated from connective tissues are pluripotent and differentiate into phenotypes of connective tissue cell lineages (osteoblasts, chondrocytes, and adipocytes) in vitro and in vivo. They have been used to treat mouse models of connective tissue disease such as lumican-null (Lum) and mucopolysaccharidosis (Gusb) mice. Mesenchymal stem cells have unique immunosuppressive properties allowing evasion of host rejection; thus, they are valuable tools for cell therapy of congenital and acquired diseases involving immune dysfunction of multiple tissues including ocular surface tissues (cornea)...
November 2016: Cornea
https://www.readbyqxmd.com/read/27627554/identification-of-small-peptides-of-acidic-collagen-extracts-from-silver-carp-skin-and-their-therapeutic-relevance
#15
Diana Wojtkowiak, Andrzej F Frydrychowski, Jakub Hadzik, Marzena Dominiak
BACKGROUND: Low-temperature techniques that prevent protein denaturation are being used to extract collagen from fish skin for cosmetic purposes. These extracts contain collagen with its triple helix structure preserved, as well as a number of other proteins. OBJECTIVES: The aim of the study was to investigate collagen extracts from the skin of silver carp for the presence of small-molecule peptides. MATERIAL AND METHODS: Liquid chromatography-mass spectrometry (HPLC-MS) was performed to analyze collagen extracts from silver carp skin for the presence of small-molecule peptides...
March 2016: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
https://www.readbyqxmd.com/read/27601940/differential-proteins-among-normal-cervix-cells-and-cervical-cancer-cells-with-hpv-16-infection-through-mass-spectrometry-based-proteomics-2d-dige-in-women-from-southern-m%C3%A3-xico
#16
Idanya Serafín-Higuera, Olga Lilia Garibay-Cerdenares, Berenice Illades-Aguiar, Eugenia Flores-Alfaro, Marco Antonio Jiménez-López, Pavel Sierra-Martínez, Luz Del Carmen Alarcón-Romero
BACKGROUND: Cervical cancer (CC) is the fourth most common cancer in women worldwide with an estimated 528,000 new cases in 2012. The same year México had an incidence of 13,960 and a mortality of 4769 cases. There are several diagnosis methods of CC; among the most frequents are the conventional Pap cytology (Pap), colposcopy, and visual inspection with acetic acid (VIA), histopathological examination, tests of imaging and detection of high-risk papilloma virus (HR-HPV) with molecular tests (PCR, hybridization, sequencing)...
2016: Proteome Science
https://www.readbyqxmd.com/read/27569372/small-leucine-rich-repeat-proteoglycans-in-corneal-inflammation-and-wound-healing
#17
REVIEW
Jihane Frikeche, George Maiti, Shukti Chakravarti
The small leucine rich repeat proteoglycans are major components of the cornea. Lumican, keratocan, decorin, biglycan and osteoglycin are present throughout the adult corneal stroma, and fibromodulin in the peripheral limbal area. In the cornea literature these proteoglycan have been reviewed as structural, collagen fibril-regulating proteins of the cornea. However, these proteoglycans are members of the leucine-rich-repeat superfamily, and share structural similarities with pathogen recognition toll-like receptors...
October 2016: Experimental Eye Research
https://www.readbyqxmd.com/read/27538499/the-proteome-of-normal-human-retrobulbar-optic-nerve-and-sclera
#18
Pingbo Zhang, Rabia Karani, Randi L Turner, Craig Dufresne, Sara Ferri, Jennifer E Van Eyk, Richard D Semba
The optic nerve is a white matter tract that conveys visual information to the brain. The sclera comprises the white, protective outer layer of the eye. A characterization of the proteome of normal human retrobulbar optic nerve and sclera may facilitate studies of the eye. We conducted a proteomic analysis of optic nerve and sclera from five adults. Proteins were fractionated using SDS-PAGE. After in-gel digestion, peptides were analyzed using LC-MS/MS on an Orbitrap Elite mass spectrometer. We identified 2711 non-redundant proteins in retrobulbar optic nerve and 1945 non-redundant proteins in sclera...
October 2016: Proteomics
https://www.readbyqxmd.com/read/27503167/development-of-myotendinous-like-junctions-that-anchor-cardiac-valves-requires-fibromodulin-and-lumican
#19
Loren E Dupuis, Lorna Doucette, A Kittrell Rice, Ashton E Lancaster, Matthew G Berger, Shukti Chakravarti, Christine B Kern
BACKGROUND: There are many patients that exhibit connective tissue related cardiac malformations but do not have mutations in collagen genes. The Small Leucine Rich Proteoglycans (SLRP) fibromodulin (FMOD) and lumican (LUM) bind collagen and regulate fibril assembly in other biological contexts. RESULTS: FMOD deficient mice and double deficient FMOD; LUM mice exhibited anomalies in regions where cardiac valve tissue interdigitates with adjacent muscle for support...
October 2016: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/27500424/glycoproteomic-analysis-of-malignant-ovarian-cancer-ascites-fluid-identifies-unusual-glycopeptides
#20
Suzanne Miyamoto, L Renee Ruhaak, Carol Stroble, Michelle R Salemi, Brett Phinney, Carlito B Lebrilla, Gary S Leiserowitz
Ovarian cancer is a major cause of cancer mortality among women, largely due to late diagnosis of advanced metastatic disease. More extensive molecular analysis of metastatic ovarian cancer is needed to identify post-translational modifications of proteins, especially glycosylation that is particularly associated with metastatic disease to better understand the metastatic process and identify potential therapeutic targets. Glycoproteins in ascites fluid were enriched by affinity binding to lectins (ConA or WGA) and other affinity matrices...
September 2, 2016: Journal of Proteome Research
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