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Lumican

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https://www.readbyqxmd.com/read/28332606/lumican-effectively-regulates-the-estrogen-receptors-associated-functional-properties-of-breast-cancer-cells-expression-of-matrix-effectors-and-epithelial-to-mesenchymal-transition
#1
Konstantina Karamanou, Marco Franchi, Zoi Piperigkou, Corinne Perreau, Francois-Xavier Maquart, Demitrios H Vynios, Stéphane Brézillon
Lumican is a small leucine-rich proteoglycan that has been shown to contribute in several physiological processes, but also to exert anticancer activity. On the other hand, it has been recently shown that knockdown of the estrogen receptor α (ERα) in low invasive MCF-7 (ERα+) breast cancer cells and the suppression of ERβ in highly aggressive MDA-MB-231 (ERβ+) cells significantly alter the functional properties of breast cancer cells and the gene expression profile of matrix macromolecules related to cancer progression and cell morphology...
March 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28222169/decellularized-wharton-s-jelly-from-human-umbilical-cord-as-a-novel-3d-scaffolding-material-for-tissue-engineering-applications
#2
Sushma Jadalannagari, Gabriel Converse, Christopher McFall, Eric Buse, Michael Filla, Maria T Villar, Antonio Artigues, Adam J Mellot, Jinxi Wang, Michael S Detamore, Richard A Hopkins, Omar S Aljitawi
In tissue engineering, an ideal scaffold attracts and supports cells thus providing them with the necessary mechanical support and architecture as they reconstruct new tissue in vitro and in vivo. This manuscript details a novel matrix derived from decellularized Wharton's jelly (WJ) obtained from human umbilical cord for use as a scaffold for tissue engineering application. This decellularized Wharton's jelly matrix (DWJM) contained 0.66 ± 0.12 μg/mg sulfated glycosaminoglycans (GAGs), and was abundant in hyaluronic acid, and completely devoid of cells...
2017: PloS One
https://www.readbyqxmd.com/read/28181591/lumican-peptides-rational-design-targeting-alk5-tgfbri
#3
Tarsis Ferreira Gesteira, Vivien J Coulson-Thomas, Yong Yuan, Jianhua Zhang, Helena B Nader, Winston W-Y Kao
Lumican, a small leucine rich proteoglycan (SLRP), is a component of extracellular matrix which also functions as a matrikine regulating multiple cell activities. In the cornea, lumican maintains corneal transparency by regulating collagen fibrillogenesis, promoting corneal epithelial wound healing, regulating gene expression and maintaining corneal homeostasis. We have recently shown that a peptide designed from the 13 C-terminal amino acids of lumican (LumC13) binds to ALK5/TGFBR1 (type1 receptor of TGFβ) to promote wound healing...
February 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28138416/retinoic-acid-enhances-the-differentiation-of-adipose-derived-stem-cells-to-keratocytes-in-vitro
#4
Amy P Lynch, Mark Ahearne
PURPOSE: All-trans retinoic acid (RA) supplementation was investigated as a method of enhancing the differentiation of human adipose-derived stem cells (ASCs) to corneal keratocytes in vitro, in combination with a chemically defined serum-free medium. METHODS: Adipose-derived stem cells were cultured in monolayer and supplemented with 0.1, 1, or 10 μM RA for 14 days. The effects of RA on cell proliferation, migration, and extracellular matrix (ECM) accumulation were evaluated...
January 2017: Translational Vision Science & Technology
https://www.readbyqxmd.com/read/28121998/rheumatic-heart-disease-and-myxomatous-degeneration-differences-and-similarities-of-valve-damage-resulting-from-autoimmune-reactions-and-matrix-disorganization
#5
Carlo de Oliveira Martins, Lea Demarchi, Frederico Moraes Ferreira, Pablo Maria Alberto Pomerantzeff, Carlos Brandao, Roney Orismar Sampaio, Guilherme Sobreira Spina, Jorge Kalil, Edecio Cunha-Neto, Luiza Guilherme
Autoimmune inflammatory reactions leading to rheumatic fever (RF) and rheumatic heart disease (RHD) result from untreated Streptococcus pyogenes throat infections in individuals who exhibit genetic susceptibility. Immune effector mechanisms have been described that lead to heart tissue damage culminating in mitral and aortic valve dysfunctions. In myxomatous valve degeneration (MXD), the mitral valve is also damaged due to non-inflammatory mechanisms. Both diseases are characterized by structural valve disarray and a previous proteomic analysis of them has disclosed a distinct profile of matrix/structural proteins differentially expressed...
2017: PloS One
https://www.readbyqxmd.com/read/28099503/3d-functional-corneal-stromal-tissue-equivalent-based-on-corneal-stromal-stem-cells-and-multi-layered-silk-film-architecture
#6
Chiara E Ghezzi, Benedetto Marelli, Fiorenzo G Omenetto, James L Funderburgh, David L Kaplan
The worldwide need for human cornea equivalents continues to grow. Few clinical options are limited to allogenic and synthetic material replacements. We hypothesized that tissue engineered human cornea systems based on mechanically robust, patterned, porous, thin, optically clear silk protein films, in combination with human corneal stromal stem cells (hCSSCs), would generate 3D functional corneal stroma tissue equivalents, in comparison to previously developed 2D approaches. Silk film contact guidance was used to control the alignment and distribution of hCSSCs on RGD-treated single porous silk films, which were then stacked in an orthogonally, multi-layered architecture and cultured for 9 weeks...
2017: PloS One
https://www.readbyqxmd.com/read/28097378/-molecular-pathogenesis-of-duchenne-muscular-dystrophy-related-fibrosis
#7
K Ohlendieck, D Swandulla
Progressive myofibrosis plays a key role in Duchenne muscular dystrophy. The dystrophic loss of contractile cells triggers a relatively nonspecific restructuring of the surrounding mesenchyme. The increase in connective and fatty tissue leads to muscular weakness and is therefore of critical importance for the cellular pathogenesis of muscular dystrophy. The systematic biochemical analysis of fibrosis using comparative proteomics has identified a number of extracellular matrix proteins that are indirectly involved in muscular dystrophy...
January 17, 2017: Der Pathologe
https://www.readbyqxmd.com/read/28059449/irx3-and-bmp2-regulate-mouse-mesenchymal-cell-chondrogenic-differentiation-in-both-a-sox9-dependent-and-independent-manner
#8
Yoshihiro Tamamura, Kenichi Katsube, Hisashi Mera, Maki Itokazu, Shigeyuki Wakitani
Sox9, a master regulator of cartilage development, controls the cell fate decision to differentiate from mesenchymal to chondrogenic cells. In addition, Sox9 regulates the proliferation and differentiation of chondrocytes, as well as the production of cartilage-specific proteoglycans. The existence of Sox9-independent mechanisms in cartilage development remains to be determined. Here, we attempted to identify genes involved in such putative mechanisms via microarray analysis using a mouse chondrogenic cell line, N1511...
January 6, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28054366/the-role-of-molecular-pain-biomarkers-in-temporomandibular-joint-internal-derangement
#9
REVIEW
Malin Ernberg
There is evidence that low-grade inflammation may be responsible for pain and development of degenerative changes in temporomandibular joint internal derangement. This article reviews the current knowledge of the molecular mechanisms behind TMJ internal derangements. A non-systematic search was carried out in PubMed, Embase, and the Cochrane library for studies regarding pathophysiological mechanisms behind internal derangements focusing on pain mediating inflammatory and cartilage degrading molecules. Recent data suggests that release of cytokines may be the key event for pain and cartilage destruction in TMJ internal derangements...
January 5, 2017: Journal of Oral Rehabilitation
https://www.readbyqxmd.com/read/28008374/comparative-proteomics-analysis-of-human-osteosarcoma-by-2d-dige-with-maldi-tof-tof-ms
#10
Guoxiang Wang, Zhengyao Zhang, Maoguang Yang, Bo Xu, Qi Gao, Xiaoyu Yang
Osteosarcoma (OS) is the most common primary malignant tumor of bone and the third most common cancer in childhood and adolescence. However, controversy concerning the ideal combination of chemotherapy agents ensued throughout the last quarter of the 20th century because of conflicting and often nonrandomized data. Collaborative efforts to increase understanding of the biology of osteosarcoma and the use of preclinical models to test novel protein targets will be critical to identify the path toward improving outcomes for patients...
November 2016: Journal of Bone Oncology
https://www.readbyqxmd.com/read/27939890/lumican-null-mice-are-susceptible-to-aging-and-isoproterenol-induced-myocardial-fibrosis
#11
Shao-Wei Chen, Ying-Chang Tung, Shih-Ming Jung, Yen Chu, Pyng-Jing Lin, Winston W-Y Kao, Pao-Hsien Chu
With aging and stress, the myocardium undergoes structural remodeling, often leading to fibrosis. The purpose of this study is to examine whether lumican, one of the class II small leucine-rich proteoglycans, has a protective role in cardiac remodeling and fibrosis. In attempts to elucidate the hypothesis that lumican may have a protective role in cardiac remodeling and fibrosis, we compared the cardiac phenotypes of young (3-month-old) and elder (6-month- and 12-month-old) lumican-null (Lum(-/-)) mice. Extra-cellular matrix remodeling and apoptosis are examined to determine the roles of lumican on age-dependent cardiac fibrosis induced by isoproterenol...
January 22, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27852402/-changes-of-ocular-biological-parameters-and-lumican-expression-in-the-monocularly-deprivation-myopic-model-of-mutant-lumican-transgenic-mice
#12
M S Sun, Y Z Song, F J Zhang, J Tao, Y B Liu
Objective: To investigate ocular changes in the monocularly deprivation myopic model of mutant Lumican transgenic mice. Comparing influences on biological parameters and sclera development between Lumican transgenic and form deprivation mice, and to prepare for further study of pathogenesis of pathological myopia (PM). Methods: Experimental research. Lumican transgenic mice and wild mice were monocularly lid-sutured at ten days after birth. All eyes were divided into 6 groups, group A(32 eyes): control eyes in transgenic mice; group B(34 eyes): sutured eyes in transgenic mice; group C(34 eyes): fellow eyes in transgenic mice; group D(28 eyes): control eyes in wild mice; group E(32 eyes): sutured eyes in wild mice; group F(32 eyes): fellow eyes in wild mice...
November 11, 2016: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
https://www.readbyqxmd.com/read/27826181/analysis-of-the-association-between-the-lum-rs3759223-variant-and-high-myopia-in-a-japanese-population
#13
Shintaro Okui, Akira Meguro, Masaki Takeuchi, Takahiro Yamane, Eiichi Okada, Yasuhito Iijima, Nobuhisa Mizuki
PURPOSE: Many studies have investigated the relationship of the lumican gene (LUM) rs3759223 variant with the risk of high myopia, but the results have been inconsistent and inconclusive. In this study, we investigated whether LUM rs3759223 is associated with high myopia in a Japanese population. METHODS: We recruited 1,585 Japanese patients with high myopia (spherical equivalent [SE] <-9.00 diopters [D]) and 1,011 Japanese healthy controls (SE ≥-1.00 D). The rs3759223 variant was genotyped using the TaqMan assay, and the allelic and genotypic diversity among cases and controls was analyzed according to the SE level...
2016: Clinical Ophthalmology
https://www.readbyqxmd.com/read/27769536/identification-of-the-most-abundant-proteins-in-equine-amniotic-fluid-by-a-proteomic-approach
#14
Gloria Isani, Enea Ferlizza, Aurora Cuoghi, Elisa Bellei, Emanuela Monari, Barbara Bianchin Butina, Carolina Castagnetti
Characterisation of the physiologic equine amniotic fluid (AF) proteome is a prerequisite to study its changes during diseases and discover new biomarkers. The aim of this study was to identify by a proteomic approach the most abundant proteins of equine AF. AF samples were collected at parturition from 24 healthy mares that delivered healthy foals. All samples were subjected to sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) on 4-12% gels. A pool of the 24 samples, after SDS-PAGE, was cut in 25 slices, trypsin-digested and analysed by mass spectrometry (MS) for protein identification...
November 2016: Animal Reproduction Science
https://www.readbyqxmd.com/read/27711221/enlargement-of-the-axial-length-and-altered-ultrastructural-features-of-the-sclera-in-a-mutant-lumican-transgenic-mouse-model
#15
Yanzheng Song, Fengju Zhang, Yanyan Zhao, Mingshen Sun, Jun Tao, Yanchuang Liang, Ling Ma, Yanqiu Yu, Jianhua Wang, Junfeng Hao
Lumican (LUM) is a candidate gene for myopia in the MYP3 locus. In this study, a mutant lumican (L199P) transgenic mouse model was established to investigate the axial length changes and ultrastructural features of the sclera. The mouse model was established by pronuclear microinjection. Transgenic mice and wild-type B6 mice were killed at eight weeks of age. Gene expression levels of LUM and collagen type I (COL1) in the sclera were analyzed by quantitative real-time polymerase chain reaction (qPCR), and the protein levels were assessed by Western blot analysis...
2016: PloS One
https://www.readbyqxmd.com/read/27706836/identifying-nonalcoholic-fatty-liver-disease-patients-with-active-fibrosis-by-measuring-extracellular-matrix-remodeling-rates-in-tissue-and-blood
#16
Martin L Decaris, Kelvin W Li, Claire L Emson, Michelle Gatmaitan, Shanshan Liu, Yenny Wang, Edna Nyangau, Marc Colangelo, Thomas E Angel, Carine Beysen, Jeffrey Cui, Carolyn Hernandez, Len Lazaro, David A Brenner, Scott M Turner, Marc K Hellerstein, Rohit Loomba
Excess collagen synthesis (fibrogenesis) in the liver plays a causal role in the progression of nonalcoholic fatty liver disease (NAFLD). Methods are needed to identify patients with more rapidly progressing disease and to demonstrate early response to treatment. We describe here a novel method to quantify hepatic fibrogenesis flux rates both directly in liver tissue and noninvasively in blood. Twenty-one patients with suspected NAFLD ingested heavy water ((2) H2 O, 50-mL aliquots) two to three times daily for 3-5 weeks prior to a clinically indicated liver biopsy...
January 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/27693693/lumican-alleviates-hypertrophic-scarring-by-suppressing-integrin-fak-signaling
#17
Yuqian Zhao, Xueyong Li, Xiaoli Xu, Zhi He, Lei Cui, Xiaoxing Lv
Hypertrophic scarring (HS) is an overcompensation of wound healing that increases the risk of cosmetic disfigurement and functional impairment. No gold standard has been established for the treatment or prevention of HS. Our study aims to elucidate the expression and function of lumican in the pathogenesis of HS as well as the underlying mechanism involved in this procedure. An animal model of HS (rabbit ear) was established, and the Ad-lumican vectors were locally injected. Primary fibroblasts isolated from patients with hypertrophic burn scars were used in vitro...
September 29, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27685949/corneal-keratocyte-transition-to-mesenchymal-stem-cell-phenotype-and-reversal-using-serum-free-medium-supplemented-with-fgf-2-tgf-%C3%A3-3-and-retinoic-acid
#18
Laura E Sidney, Andrew Hopkinson
Keratocytes of the corneal limbal stroma can derive populations of mesenchymal stem cells (MSC) when expanded in vitro. However, once a corneal MSC (cMSC) phenotype is achieved, regaining the keratocyte phenotype can be challenging, and there is no standardised differentiation medium. Here, we investigated the transition of keratocytes to cMSC and compared different supplements in their ability to return cMSC to a keratocyte phenotype. Immunofluorescence and RT-qPCR demonstrated in vivo keratocyte expression of ALDH3A1, CD34 and keratocan, but not any of the typical MSC markers (CD73, CD90, CD105)...
September 29, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27682997/lumican-negatively-controls-the-pathogenicity-of-murine-encephalitic-th17-cells
#19
Eliseo F Castillo, Handong Zheng, Christian Van Cabanlong, Fei Dong, Yan Luo, Yi Yang, Meilian Liu, Winston W-Y Kao, Xuexian O Yang
TH17 cells play an essential role in the development of both human multiple sclerosis and animal experimental autoimmune encephalomyelitis (EAE). Nevertheless, it is not well understood how the pathogenicity of TH17 cells is controlled in the autoimmune neuroinflammation. In vitro, we found Lumican (Lum), an extracellular matrix (ECM) protein, is selectively expressed by TH17 cells among tested murine TH subsets. Lum deficiency leads to earlier onset and enhanced severity of EAE. This enhanced disease in Lum-deficient mice is associated with increased production of IL-17 and IL-21 and decreased TH17 cell apoptosis...
December 2016: European Journal of Immunology
https://www.readbyqxmd.com/read/27672590/construction-of-adenovirus-vectors-encoding-the-lumican-gene-by-gateway-recombinant-cloning-technology
#20
Gui-Fang Wang, Bing Qi, Lei-Lei Tu, Lian Liu, Guo-Cheng Yu, Jing-Xiang Zhong
AIM: To construct adenovirus vectors of lumican gene by gateway recombinant cloning technology to further understand the role of lumican gene in myopia. METHODS: Gateway recombinant cloning technology was used to construct adenovirus vectors. The wild-type (wt) and mutant (mut) forms of the lumican gene were synthesized and amplified by polymerase chain reaction (PCR). The lumican cDNA fragments were purified and ligated into the adenovirus shuttle vector pDown-multiple cloning site (MCS)-/internal ribozyme entry site (IRES)/enhanced green fluorescent protein (EGFP)...
2016: International Journal of Ophthalmology
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