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https://www.readbyqxmd.com/read/29340639/biomechanical-rigidity-and-quantitative-proteomics-analysis-of-segmental-regions-of-the-trabecular-meshwork-at-physiologic-and-elevated-pressures
#1
Janice A Vranka, Julia A Staverosky, Ashok P Reddy, Phillip A Wilmarth, Larry L David, Ted S Acott, Paul Russell, Vijay Krishna Raghunathan
Purpose: The extracellular matrix (ECM) of the trabecular meshwork (TM) modulates resistance to aqueous humor outflow, thereby regulating IOP. Glaucoma, a leading cause of irreversible blindness worldwide, is associated with changes in the ECM of the TM. The elastic modulus of glaucomatous TM is larger than age-matched normal TM; however, the biomechanical properties of segmental low (LF) and high flow (HF) TM regions and their response to elevated pressure, are unknown. Methods: We perfused human anterior segments at two pressures using an ex vivo organ culture system...
January 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29338928/immunoproteomic-identification-of-antigenic-candidate-campylobacter-jejuni-and-human-peripheral-nerve-proteins-involved-in-guillain-barr%C3%A3-syndrome
#2
Aida Loshaj-Shala, Mara Colzani, Katerina Brezovska, Ana Poceva Panovska, Ljubica Suturkova, Giangiacomo Beretta
Immunoproteomics is become a potent methodology used for identifying immunoreactive proteins. In this study, an immunoproteomic approach based on 2-dimensional gel electrophoresis (2D-PAGE) and immunoblotting combined with high resolution mass spectrometry (MS) was used to identify immunoreactive proteins that might be involved in mechanisms of Guillain-Barré syndrome (GBS) development, regardless of their potential reciprocal molecular mimicry. Proteins isolated from C. jejuni and human peripheral nerve tissue (HPN) were separated with 2D SDS-PAGE and subjected to western blotting using serum samples from GBS patients...
January 9, 2018: Journal of Neuroimmunology
https://www.readbyqxmd.com/read/29323130/in-vivo-effect-of-opticin-deficiency-in-cartilage-in-a-surgically-induced-mouse-model-of-osteoarthritis
#3
Aina Farrán, Gladys Valverde-Franco, Laura Tío, Bertrand Lussier, Hassan Fahmi, Jean-Pierre Pelletier, Paul N Bishop, Jordi Monfort, Johanne Martel-Pelletier
The SLRP opticin (OPTC) has been demonstrated to be produced and degraded in osteoarthritic (OA) human cartilage. Here, we investigated the in vivo effect of OPTC deficiency in OA cartilage. OA was induced in 10-week-old Optc -/- and Optc +/+ mice. Ten weeks post-surgery, cartilage was processed for histology and immunohistochemistry. SLRP expression was determined in non-operated mouse cartilage. OA Optc -/- demonstrated significant protection against cartilage degradation. Data revealed that in non-operated Optc -/- cartilage, expression of SLRPs lumican and epiphycan was up-regulated at day 3 and in 10-week-olds (p ≤ 0...
January 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29177105/investigating-molecular-connections-of-non-alcoholic-fatty-liver-disease-with-associated-pathological-conditions-in-west-virginia-for-biomarker-analysis
#4
Dana L Sharma, Hari Vishal Lakhani, Rebecca L Klug, Brian Snoad, Rawan El-Hamdani, Joseph I Shapiro, Komal Sodhi
Non-alcoholic fatty liver disease (NAFLD) is a disease characterized by a steatosis of the liver that may progress to more serious pathological conditions including: nonalcoholic steatohepatitis (NASH), fibrosis, and cirrhosis. As the prevalence of NAFLD has increased worldwide in recent years, pathophysiology and risk factors associated with disease progression of NAFLD are at the focus of many studies. NAFLD is related to and shares common serum biomarkers with cardiovascular disease (CVD), type 2 diabetes mellitus (T2DM), obesity, and metabolic syndrome (MetS)...
October 2017: Journal of Clinical & Cellular Immunology
https://www.readbyqxmd.com/read/29132420/substance-p-and-patterned-silk-biomaterial-stimulate-periodontal-ligament-stem-cells-to-form-corneal-stroma-in-a-bioengineered-three-dimensional-model
#5
Jialin Chen, Wei Zhang, Peyman Kelk, Ludvig J Backman, Patrik Danielson
BACKGROUND: We aimed to generate a bioengineered multi-lamellar human corneal stroma tissue in vitro by differentiating periodontal ligament stem cells (PDLSCs) towards keratocytes on an aligned silk membrane. METHODS: Human PDLSCs were isolated and identified. The neuropeptide substance P (SP) was added in keratocyte differentiation medium (KDM) to evaluate its effect on keratocyte differentiation of PDLSCs. PDLSCs were then seeded on patterned silk membrane and cultured with KDM and SP...
November 13, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29129081/identification-of-mature-atherosclerotic-plaque-proteome-signatures-using-data-independent-acquisition-mass-spectrometry
#6
Nicole Hansmeier, Josef Buttigieg, Pankaj Kumar, Shaneen Pelle, Kyoo Yoon Choi, David Kopriva, Tzu-Chiao Chao
Atherosclerosis is a chronic inflammatory disease with complex pathobiology and one of the most common causes of cardiovascular events. The process is characterized by complex vascular remodeling processes that require the actions of numerous proteins. The composition of atherosclerotic plaque is increasingly recognized as a major factor governing the occurrence of cardiovascular or neurological symptoms. In order to gain deeper insights in the composition of atherosclerotic plaques, we created quantitative proteome profiles of advanced plaque tissues of six male patients undergoing carotid endarterectomy for stroke prevention...
November 11, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/29121102/histopathologic-and-immunohistochemical-features-of-capsular-tissue-around-failed-ahmed-glaucoma-valves
#7
Alka Mahale, Fatma Fikri, Khitam Al Hati, Sami Al Shahwan, Ibrahim Al Jadaan, Hind Al Katan, Rajiv Khandekar, Azza Maktabi, Deepak P Edward
Impervious encapsulation around Ahmed glaucoma valve (AGV) results in surgical failure raising intraocular pressure (IOP). Dysregulation of extracellular matrix (ECM) molecules and cellular factors might contribute to increased hydraulic resistance to aqueous drainage. Therefore, we examined these molecules in failed AGV capsular tissue. Immunostaining for ECM molecules (collagen I, collagen III, decorin, lumican, chondroitin sulfate, aggrecan and keratan sulfate) and cellular factors (αSMA and TGFβ) was performed on excised capsules from failed AGVs and control tenon's tissue...
2017: PloS One
https://www.readbyqxmd.com/read/29098025/lumican-expression-in-gastric-cancer-and-its-association-with-biological-behavior-and-prognosis
#8
Li Chen, Yuxin Zhang, Yanjiao Zuo, Fei Ma, Hongjiang Song
The aim of the present study was to investigate the expression of lumican in human gastric cancer and adjacent normal gastric tissues, and study its association with clinicopathological characteristics and survival rate. By using immunohistochemistry, the lumican expression patterns in 146 cases of gastric cancer with various clinicopathological characteristics 55 adjacent normal gastric tissue specimens was studied. And the significance of lumican expression regarding the biological behavior and survival of patients was evaluated...
November 2017: Oncology Letters
https://www.readbyqxmd.com/read/29062769/the-association-of-lumican-polymorphisms-and-high-myopia-in-a-southern-chinese-population
#9
Gui-Fang Wang, Qing-Shan Ji, Bing Qi, Guo-Cheng Yu, Lian Liu, Jing-Xiang Zhong
AIM: To investigate the correlation between lumican (LUM) gene and high myopia in a Southern Chinese population. METHODS: The study comprised of 95 high myopia patients with a spherical equivalent ≤-6.5 diopters (D). The control group recruited 95 individuals with a spherical equivalent ranging from -0.5 D to +0.5 D. Direct sequencing was used to detect the single nucleotide polymorphisms (SNPs) of LUM gene in coding region. Genotype distributions were tested for Hardy-Weinberg disequilibrium...
2017: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/29051516/stromal-striae-a-new-insight-into-corneal-physiology-and-mechanics
#10
Kate Grieve, Djida Ghoubay, Cristina Georgeon, Gael Latour, Amir Nahas, Karsten Plamann, Caroline Crotti, Romain Bocheux, Marie Borderie, Thu-Mai Nguyen, Felipe Andreiuolo, Marie-Claire Schanne-Klein, Vincent Borderie
We uncover the significance of a previously unappreciated structural feature in corneal stroma, important to its biomechanics. Vogt striae are a known clinical indicator of keratoconus, and consist of dark, vertical lines crossing the corneal depth. However we detected stromal striae in most corneas, not only keratoconus. We observed striae with multiple imaging modalities in 82% of 118 human corneas, with pathology-specific differences. Striae generally depart from anchor points at Descemet's membrane in the posterior stroma obliquely in a V-shape, whereas in keratoconus, striae depart vertically from posterior toward anterior stroma...
October 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29023489/spatiotemporal-variations-in-gene-expression-histology-and-biomechanics-in-an-ovine-model-of-tendinopathy
#11
Sara Biasutti, Andrew Dart, Margaret Smith, Carina Blaker, Elizabeth Clarke, Leo Jeffcott, Christopher Little
Flexor tendinopathy is a common problem affecting humans and animals. Tendon healing is poorly understood and the outcomes of conservative and surgical management are often suboptimal. While often considered a localized injury, recent evidence indicates that in the short term, tendinopathic changes are distributed widely throughout the tendon, remote from the lesion itself. Whether these changes persist throughout healing is unknown. The aim of this study was to document gene expression, histopathological and biomechanical changes that occur throughout the superficial digital flexor tendon (SDFT) up to 16 weeks post-injury, using an ovine surgical model of tendinopathy...
2017: PloS One
https://www.readbyqxmd.com/read/28980718/identification-of-over-expressed-proteins-in-oral-submucous-fibrosis-by-proteomic-analysis
#12
Tapasi Das, Chandraday Prodhan, Santunu Patsa, Jay Gopal Ray, Keya Chaudhuri
Early detection and identification of oral pre-malignancy or malignancy help in management of the disease and improve survival rates. Oral submucous fibrosis (OSMF) is a major threat to public health worldwide and especially in Southeast Asian countries. Identification of biomarkers is a necessary step towards early diagnosis and treatment. In this study, differentially expressed proteins between oral submucous fibrotic tissue and normal control tissues were recorded by proteomic analysis using two dimensional electrophoresis (2DE) and MALDI TOF mass spectrometry...
October 5, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28958189/small-leucine-rich-repeat-proteoglycans-associated-with-mature-insoluble-elastin-serve-as-binding-sites-for-galectins
#13
Aiko Itoh, Yasuhiro Nonaka, Takashi Ogawa, Takanori Nakamura, Nozomu Nishi
We previously reported that galectin-9 (Gal-9), an immunomodulatory animal lectin, could bind to insoluble collagen preparations and exerted direct cytocidal effects on immune cells. In the present study, we found that mature insoluble elastin is capable of binding Gal-9 and other members of the human galectin family. Lectin blot analysis of a series of commercial water-soluble elastin preparations, PES-(A) ~ PES-(E), revealed that only PES-(E) contained substances recognized by Gal-9. Gal-9-interacting substances in PES-(E) were affinity-purified, digested with trypsin and then analyzed by reversed-phase HPLC...
November 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28855568/plasma-biomarker-proteins-for-detection-of-human-growth-hormone-administration-in-athletes
#14
Sock-Hwee Tan, Albert Lee, Dana Pascovici, Natasha Care, Vita Birzniece, Ken Ho, Mark P Molloy, Alamgir Khan
Human growth hormone (GH) is a naturally occurring hormone secreted by the pituitary gland with anabolic and growth-promoting activities. Since an increased availability of recombinant GH (rGH) for the treatment of GH-deficient patients, GH has been abused in sports and it is prohibited. "GH-isoform" and "biomarkers" tests are currently available for detection of GH abuse in sports, however both methods suffer from shortcomings. Here, we report on a proteomic approach to search for novel protein biomarkers associated with rGH administration in non-elite athletes...
August 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28794454/lumican-delays-melanoma-growth-in-mice-and-drives-tumor-molecular-assembly-as-well-as-response-to-matrix-targeted-tax2-therapeutic-peptide
#15
Albin Jeanne, Valérie Untereiner, Corinne Perreau, Isabelle Proult, Cyril Gobinet, Camille Boulagnon-Rombi, Christine Terryn, Laurent Martiny, Stéphane Brézillon, Stéphane Dedieu
Lumican is a small leucine-rich proteoglycan (SLRP) being known as a key regulator of collagen fibrillogenesis. However, little attention has been given so far in studying its influence on tumor-associated matrix architecture. Here, we investigate the role of host lumican on tumor matrix organization as well as on disease progression considering an immunocompetent model of melanoma implanted in Lum (-/-) vs. wild type syngeneic mice. Conjointly, lumican impact on tumor response to matrix-targeted therapy was evaluated considering a previously validated peptide, namely TAX2, that targets matricellular thrombospondin-1...
August 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28789706/small-leucine-rich-proteoglycans-slrps-in-the-endometrium-of-polycystic-ovary-syndrome-women-a-pilot-study
#16
Ricardo Santos Simões, José Maria Soares-Jr, Manuel J Simões, Helena B Nader, Maria Cândida P Baracat, Gustavo Arantes R Maciel, Paulo C Serafini, Ricardo Azziz, Edmund C Baracat
BACKGROUND: Small leucine-rich proteoglycans (SLRPs) play an important role in tissue homeostasis and cell proliferation since these proteoglycans sequester multiple growth factors. However, the content of SLRPs in the endometrium of polycystic ovary syndrome (PCOS) women is unknown. Our purpose was to test the hypothesis that excessive endometrial proliferation in PCOS may be partly related to abnormalities in SLRPs. METHODS: In a cross section study a total of 20 endometrial samples were collected from 10 patients with PCOS and 10 ovulatory women during their proliferative (pre-ovulatory) phase...
August 8, 2017: Journal of Ovarian Research
https://www.readbyqxmd.com/read/28542982/cancer-associated-fibroblast-derived-lumican-promotes-gastric-cancer-progression-via-the-integrin-%C3%AE-1-fak-signaling-pathway
#17
Xiaofeng Wang, Quan Zhou, Zhenjia Yu, Xiongyan Wu, Xuehua Chen, Jianfang Li, Chen Li, Min Yan, Zhenggang Zhu, Bingya Liu, Liping Su
Gastric cancer (GC) is one of the most frequent malignant tumors worldwide and is associated with high invasiveness, high metastasis and poor prognosis. Cancer-associated fibroblasts (CAFs), residing around tumor cells in tumor stroma, are important modifiers of tumor initiation and progression. However, the molecular mechanisms by which CAF's modulate tumor development have yet not to be characterized in GC. Here we performed tissue assay analyses identifying that Lumican, an extracellular matrix protein, is highly expressed in human gastric CAFs and its expression is positively associated with depth of invasion, lymph node metastasis, TNM stage and poor survival rate of GC...
September 1, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28535062/supramolecular-hydrogel-formation-in-a-series-of-self-assembling-lipopeptides-with-varying-lipid-chain-length
#18
V Castelletto, A Kaur, R M Kowalczyk, I W Hamley, M Reza, J Ruokolainen
The self-assembly in aqueous solution of three lipopeptides comprising a bioactive motif conjugated at the N terminus to dodecyl, tetradecyl or hexadecyl lipid chains has been examined. The bioactive motif is the peptide block YEALRVANEVTLN; a C-terminal fragment of the lumican proteoglycan. This study was motivated by our previous studies on the hexadecyl homologue C16-YEALRVANEVTLN, which showed aggregation into β-sheet structures above a critical aggregation concentration (cac), but most remarkably, we found that these aggregates were stable to dilution below the cac...
June 5, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28534517/prolonged-exposure-to-extracellular-lumican-restrains-pancreatic-adenocarcinoma-growth
#19
X Li, Y Kang, D Roife, Y Lee, M Pratt, M R Perez, B Dai, E J Koay, J B Fleming
We previously demonstrated that pancreatic stellate cells within pancreatic ductal adenocarcinoma (PDAC) stroma secrete lumican and its presence is associated with prolonged survival of patients with localized PDAC. Here, we observed that extracellular lumican decreases PDAC tumour cell growth in xenograft and syngeneic orthotopic animal models, and induces growth inhibition of low-passage human PDAC cells in a species-specific manner. PDAC cells grown in variant culture conditions and exposed to extracellular lumican display typical characterizations of cancer cell in a quiescent state, such as growth inhibition, apoptosis, G0/G1 arrest and chemoresistance...
September 21, 2017: Oncogene
https://www.readbyqxmd.com/read/28527949/microproteome-of-dentoalveolar-tissues
#20
Cristiane R Salmon, Ana Paula O Giorgetti, Adriana F Paes Leme, Romênia R Domingues, Tamara N Kolli, Brian L Foster, Francisco H Nociti
Proteomic analysis of extracellular matrices (ECM) of dentoalveolar tissues can provide insights into developmental, pathological, and reparative processes. However, targeted dissection of mineralized tissues, dental cementum (DC), alveolar bone (AB), and dentin (DE), presents technical difficulties. We demonstrate an approach combining EDTA decalcification and laser capture microdissection (LCM), followed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS), to analyze proteome profiles of these tissues...
May 17, 2017: Bone
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