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https://www.readbyqxmd.com/read/29355558/-epicatechin-stimulates-mitochondrial-biogenesis-and-cell-growth-in-c2c12-myotubes-via-the-g-protein-coupled-estrogen-receptor
#1
Aldo Moreno-Ulloa, Adriana Miranda-Cervantes, Alexei Licea-Navarro, Christina Mansour, Ernesto Beltrán-Partida, Luis Donis-Maturano, Hilda Carolina Delgado De la Herrán, Francisco Villarreal, Carolina Álvarez-Delgado
We have reported on the capacity of (-)-epicatechin ((-)-EPI) to stimulate mitochondrial biogenesis (MiB) in mouse skeletal muscle (SkM). However, the mechanisms mediating the effects of (-)-EPI are not fully understood. We previously identified a role of the G-protein coupled estrogen receptor (GPER) in modulating the vascular effects of (-)-EPI. We therefore tested the hypothesis that GPER mediates (at least in part) the stimulatory effects of (-)-EPI on MiB in SkM cells. As an in vitro model, we employed mouse SkM-derived C2C12 myoblasts differentiated into myotubes...
January 16, 2018: European Journal of Pharmacology
https://www.readbyqxmd.com/read/29337653/safety-and-immunogenicity-of-a-meningococcal-b-recombinant-vaccine-when-administered-with-routine-vaccines-to-healthy-infants-in-taiwan-a-phase-3-open-label-randomized-study
#2
Nan-Chang Chiu, Li-Min Huang, Arnold Willemsen, Chiranjiwi Bhusal, Ashwani Kumar Arora, Zenaida Reynoso Mojares, Daniela Toneatto
Neisseria meningitidis is associated with high mortality and morbidity in infants and children worldwide. This phase 3 study (NCT02173704) evaluated safety and immunogenicity of a 4-component serogroup B recombinant meningococcal vaccine (4CMenB) co-administered with routine vaccines in Taiwanese infants. In total, 225 healthy infants were randomized (2:1) to receive 4CMenB and routine vaccines (4CMenB+Routine) or routine vaccines only (Routine group) at 2, 4, 6 and 12 months of age. Routine vaccines were diphtheria-tetanus-acellular pertussis-inactivated poliovirus-Haemophilus influenzae type b, 13-valent pneumococcal, hepatitis B, measles-mumps-rubella and varicella vaccines...
January 16, 2018: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/29307192/enrofloxacin-permeation-pathways-across-the-porin-ompc
#3
Jigneshkumar Dahyabhai Prajapati, Carlos José Fernández Solano, Mathias Winterhalter, Ulrich Kleinekathöfer
In Gram-negative bacteria, the lack or quenching of antibiotics translocation across the outer membrane is one of the main factors for acquiring antibiotic resistance. An atomic level comprehension of the key features governing the transport of drugs by outer membrane protein channels will be very helpful in developing the next generation of antibiotics. In a previous study [J. D. Prajapati et al, J. Chem. Theory Comput. 2017, 13, 4553--4566], we characterized the diffusion pathway of the ciprofloxacin molecule through the outer membrane porin OmpC of E...
January 6, 2018: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/29306652/influence-of-the-surrounding-environment-in-re-naturalized-%C3%AE-barrel-membrane-proteins
#4
Maximilien Lopes-Rodrigues, Jordi Triguero, Juan Torras, Eric A Perpète, Catherine Michaux, David Zanuy, Carlos Alemán
Outer-membrane porins are currently being used to prepare bioinspired nanomembranes for selective ion transport by immobilizing them into polymeric matrices. However, the fabrication of these protein-integrated devices has been found to be strongly influenced by the instability of the β-barrel porin structure, which depends on surrounding environment. In this work, molecular dynamics simulations have been used to investigate the structural stability of a representative porin, OmpF, in three different environments: (i) aqueous solution at pH=7; (ii) a solution of neutral detergent in a concentration similar to the critical micelle concentration; and (iii) the protein embedded into a neutral detergent bilayer...
January 2, 2018: Biophysical Chemistry
https://www.readbyqxmd.com/read/29297851/ram-locus-is-a-key-regulator-to-trigger-multidrug-resistance-in-enterobacter-aerogenes
#5
Alexander Molitor, Chloë E James, Séamus Fanning, Jean-Marie Pagès, Anne Davin-Regli
PURPOSE: Several genetic regulators belonging to AraC family are involved in the emergence of MDR isolates of E. aerogenes due to alterations in membrane permeability. Compared with the genetic regulator Mar, RamA may be more relevant towards the emergence of antibiotic resistance. METHODOLOGY: Focusing on the global regulators, Mar and Ram, we compared the amino acid sequences of the Ram repressor in 59 clinical isolates and laboratory strains of E. aerogenes. Sequence types were associated with their corresponding multi-drug resistance phenotypes and membrane protein expression profiles using MIC and immunoblot assays...
January 3, 2018: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29295593/structural-and-immunological-characterization-of-novel-recombinant-momp-based-chlamydial-antigens
#6
Guillermo Madico, Olga Gursky, Jeff Fairman, Paola Massari
Chlamydia is the most common cause of bacterial sexually transmitted infections worldwide. While infections resolve with antibiotic treatment, this is often neglected in women due to frequent asymptomatic infections, leading to disease progression and severe sequelae (pelvic inflammatory disease, ectopic pregnancy, infertility). Development of a vaccine against Chlamydia is crucial. Whole organism-based vaccines have short-lived activity, serovar/subgroup-specific immunity and can cause adverse reactions in vaccinated subjects...
December 25, 2017: Vaccines
https://www.readbyqxmd.com/read/29287109/biogenesis-of-the-mitochondrial-dna-inheritance-machinery-in-the-mitochondrial-outer-membrane-of-trypanosoma-brucei
#7
Sandro Käser, Mathilde Willemin, Felix Schnarwiler, Bernd Schimanski, Daniel Poveda-Huertes, Silke Oeljeklaus, Beat Haenni, Benoît Zuber, Bettina Warscheid, Chris Meisinger, André Schneider
Mitochondria cannot form de novo but require mechanisms that mediate their inheritance to daughter cells. The parasitic protozoan Trypanosoma brucei has a single mitochondrion with a single-unit genome that is physically connected across the two mitochondrial membranes with the basal body of the flagellum. This connection, termed the tripartite attachment complex (TAC), is essential for the segregation of the replicated mitochondrial genomes prior to cytokinesis. Here we identify a protein complex consisting of three integral mitochondrial outer membrane proteins-TAC60, TAC42 and TAC40-which are essential subunits of the TAC...
December 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/29280343/the-ropae-gene-encodes-a-porin-like-protein-involved-in-copper-transit-in-rhizobium-etli-cfn42
#8
Antonio González-Sánchez, Ciro A Cubillas, Fabiola Miranda, Araceli Dávalos, Alejandro García-de Los Santos
Copper (Cu) is an essential micronutrient for all aerobic forms of life. Its oxidation states (Cu+ /Cu2+ ) make this metal an important cofactor of enzymes catalyzing redox reactions in essential biological processes. In gram-negative bacteria, Cu uptake is an unexplored component of a finely regulated trafficking network, mediated by protein-protein interactions that deliver Cu to target proteins and efflux surplus metal to avoid toxicity. Rhizobium etliCFN42 is a facultative symbiotic diazotroph that must ensure its appropriate Cu supply for living either free in the soil or as an intracellular symbiont of leguminous plants...
December 27, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/29275225/the-efflux-pump-inhibitor-phenylalanine-arginine-beta-naphthylamide-pa%C3%AE-n-increases-the-resistance-to-carbapenems-in-chilean-clinical-isolates-of-kpc-producing-k-pneumoniae
#9
Alejandra Vera-Leiva, Sergio Carrasco-Anabalón, Celia A Lima, Nicolás Villagra, Mariana Domínguez, Helia Bello-Toledo, Gerardo González-Rocha
OBJECTIVES: KPC-producing strains present a wide range of MICs of carbapenems; this variation may be due to different gene expression levels of the blaKPC and porin genes, associated with efflux-pumps and the production of ESBLs and/or AmpC β-lactamase. The aim of this study was to determine the role of efflux-pumps inhibited by PAβN in the resistance to carbapenems in Chilean clinical isolates of K. pneumoniae harbouring the gene blaKPC. METHODS: Minimum Inhibitory Concentration (MIC) was determined by the agar dilution method for imipenem, meropenem, ertapenem and ciprofloxacin in the presence and absence of PAβN (25mg/L)...
December 21, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29274802/susceptibility-testing-and-detection-of-%C3%AE-lactam-resistance-mechanisms-in-enterobacteriaceae-a-multicentre-national-proficiency-study
#10
María Díez-Aguilar, María-Carmen Conejo, María-Isabel Morosini, Núria Tormo Palop, Concepción Gimeno, Rafael Cantón, Álvaro Pascual
A nationwide study was developed to evaluate the ability of 60 Spanish clinical microbiology laboratories to predict the underlying β-lactam resistance mechanisms of 12 Enterobacteriaceae strains (CCS01-CCS12). Results obtained by two reference laboratories were compared with those reported by the participants that used their own routine susceptibility testing methodology. CLSI interpretive criteria were used in 53.3% of the centres, while 46.7% used EUCAST. The overall categorical agreement (CA) was 85.5%...
December 21, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29260856/acinetobacter-baumannii-ompa-is-a-selective-antibiotic-permeant-porin
#11
Ramkumar Iyer, Samir H Moussa, Thomas F Durand-Réville, Ruben Tommasi, Alita Miller
OmpAAb is a conserved, abundantly expressed outer membrane porin in Acinetobacter baumannii whose presumed role in antibiotic permeation has not been clearly demonstrated. In this report, we use a titratable heterologous expression system to express OmpAAb in isolation and demonstrate selective passage of small molecule antibiotics through OmpAAb. ETX2514, a recently discovered broad-spectrum β-lactamase inhibitor, in combination with sulbactam, is currently in clinical testing for the treatment of drug-resistant A...
December 26, 2017: ACS Infectious Diseases
https://www.readbyqxmd.com/read/29249313/in-silico-identification-and-high-throughput-screening-of-antigenic-proteins-as-candidates-for-a-mannheimia-haemolytica-vaccine
#12
Cassidy L Klima, Rahat Zaheer, Shaun R Cook, Jay Rasmussen, Trevor W Alexander, Andrew Potter, Steve Hendrick, Tim A McAllister
This study examined the use of comparative genomic analysis for vaccine design against Mannheimia haemolytica, a respiratory pathogen of ruminants. A total of 2,341genes were identified in at least half of the 23 genomes. Of these, a total of 240 were identified to code for N-terminal signal peptides with diverse sub-cellular localizations (78 periplasmic, 52 outer membrane, 15 extracellular, 13 cytoplasmic membrane and 82 unknown) and were examined in an ELISA assay using a coupled-cell free transcription/translation system for protein expressionwith antisera from cattle challenged with serovars 1, 2 or 6 of M...
January 2018: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/29234497/insertional-inactivation-of-oprd-in-carbapenem-resistant-pseudomonas-aeruginosa-strains-isolated-from-burn-patients-in-tehran-iran
#13
A Shariati, T Azimi, A Ardebili, A S Chirani, A Bahramian, A Pormohammad, M Sadredinamin, S Erfanimanesh, N Bostanghadiri, S Shams, A Hashemi
In this study, we report the insertion sequence ISPpu21 in the oprD porin gene of carbapenem-resistant Pseudomonas aeruginosa isolates from burn patients in Tehran, Iran. Antibiotic susceptibility tests for P. aeruginosa isolates were determined. Production of metallo-β-lactamases (MBLs) and carbapenemase was evaluated and the β-lactamase-encoding and aminoglycoside-modifying enzyme genes were investigated by PCR and sequencing methods. The mRNA transcription level of oprD and mex efflux pump genes were evaluated by real-time PCR...
January 2018: New Microbes and New Infections
https://www.readbyqxmd.com/read/29232112/switchable-control-of-antibiotic-activity-a-shape-shifting-tail-strategy
#14
Jinming Chang, Yi Chen, Zhou Xu, Zhonghui Wang, Qi Zeng, Haojun Fan
Bacterial resistance is emerging as a global threat, stemming partially from continuous exposure of pathogens to antibiotics of sublethal concentrations. Thus, novel molecular approaches capable of inactivating antibiotics, which prevent their final build-up in the environment, are highly desirable. Here, we report a proof-of-principle demonstration of a mechanically new strategy for switchable control of antibiotic activity, which regulates drug uptake across the outer membrane of Gram-negative bacteria by externally triggered shape shifting of a short, covalently attached "tail"...
December 12, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/29230684/imipenem-relebactam-and-meropenem-vaborbactam-two-novel-carbapenem-%C3%AE-lactamase-inhibitor-combinations
#15
REVIEW
George G Zhanel, Courtney K Lawrence, Heather Adam, Frank Schweizer, Sheryl Zelenitsky, Michael Zhanel, Philippe R S Lagacé-Wiens, Andrew Walkty, Andrew Denisuik, Alyssa Golden, Alfred S Gin, Daryl J Hoban, Joseph P Lynch, James A Karlowsky
Relebactam (formerly known as MK-7655) is a non-β-lactam, bicyclic diazabicyclooctane, β-lactamase inhibitor that is structurally related to avibactam, differing by the addition of a piperidine ring to the 2-position carbonyl group. Vaborbactam (formerly known as RPX7009) is a non-β-lactam, cyclic, boronic acid-based, β-lactamase inhibitor. The structure of vaborbactam is unlike any other currently marketed β-lactamase inhibitor. Both inhibitors display activity against Ambler class A [including extended-spectrum β-lactamases (ESBLs), Klebsiella pneumoniae carbapenemases (KPCs)] and class C β-lactamases (AmpC)...
December 12, 2017: Drugs
https://www.readbyqxmd.com/read/29223157/self-organization-of-recombinant-membrane-porin-ompf-from-yersinia-pseudotuberculosis-in-aqueous-environments
#16
E V Sidorin, V A Khomenko, N Yu Kim, P S Dmitrenok, A M Stenkova, O D Novikova, T F Solov'eva
Recombinant porin OmpF (an integral protein of bacterial outer membrane) from Yersinia pseudotuberculosis was synthesized in Escherichia coli cells as inclusion bodies. By combining the methods of anion-exchange and gel filtration chromatographies, recombinant OmpF (rOmpF) was isolated as an individual protein in its denatured state, and its characteristic properties (molecular mass, N-terminal amino acid sequence, and hydrodynamic radius of the protein in 8 M urea solution) were determined. According to the data of gel filtration, dynamic light scattering, optical spectroscopy, and binding of the hydrophobic fluorescent probe 8-anilino-1-naphthalenesulfonic acid, the rOmpF is fully unfolded in 8 M urea and exists in random coil conformation...
November 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/29216932/nanobodies-targeting-conserved-epitopes-on-the-major-outer-membrane-protein-of-campylobacter-as-potential-tools-for-control-of-campylobacter-colonization
#17
Charlotte Vanmarsenille, Inés Díaz Del Olmo, Jelle Elseviers, Gholamreza Hassanzadeh Ghassabeh, Kristof Moonens, Didier Vertommen, An Martel, Freddy Haesebrouck, Frank Pasmans, Jean-Pierre Hernalsteens, Henri De Greve
Campylobacter infections are among the most prevalent foodborne infections in humans, resulting in a massive disease burden worldwide. Broilers have been identified as the major source of campylobacteriosis and reducing Campylobacter loads in the broiler caeca has been proposed as an effective measure to decrease the number of infections in humans. Failure of current methods to control Campylobacter in broilers stresses the urgency to develop novel mitigation measures. We obtained six nanobodies with a broad specificity, that recognize strains belonging to the two most relevant species, Campylobacter jejuni and Campylobacter coli...
December 8, 2017: Veterinary Research
https://www.readbyqxmd.com/read/29211780/prevalence-and-antibiotic-resistance-of-pseudomonas-aeruginosa-in-water-samples-in-central-italy-and-molecular-characterization-of-oprd-in-imipenem-resistant-isolates
#18
Giuditta Fiorella Schiavano, Elisa Carloni, Francesca Andreoni, Silvia Magi, Maria Chironna, Giorgio Brandi, Giulia Amagliani
SCOPE: This study aimed to analyse the prevalence, antibiotic resistance and genetic relatedness of P. aeruginosa isolates obtained from potable and recreational water samples (n. 8,351) collected from different settings (swimming pools, n. 207; healthcare facilities, n 1,684; accommodation facilities, n. 1,518; municipal waterworks, n. 4,500; residential buildings, n. 235). Possible mechanisms underlying resistance to imipenem, with particular focus on those involving oprD-based uptake, were also explored...
2017: PloS One
https://www.readbyqxmd.com/read/29211019/dual-regulation-of-the-small-rna-micc-and-the-quiescent-porin-ompn-in-response-to-antibiotic-stress-in-escherichia-coli
#19
Sushovan Dam, Jean-Marie Pagès, Muriel Masi
Antibiotic resistant Gram-negative bacteria are a serious threat for public health. The permeation of antibiotics through their outer membrane is largely dependent on porin, changes in which cause reduced drug uptake and efficacy. Escherichia coli produces two major porins, OmpF and OmpC. MicF and MicC are small non-coding RNAs (sRNAs) that modulate the expression of OmpF and OmpC, respectively. In this work, we investigated factors that lead to increased production of MicC. micC promoter region was fused to lacZ, and the reporter plasmid was transformed into E...
December 6, 2017: Antibiotics
https://www.readbyqxmd.com/read/29208119/comparative-analysis-of-the-burkholderia-cenocepacia-k56-2-essential-genome-reveals-cell-envelope-functions-that-are-uniquely-required-for-survival-in-species-of-the-genus-burkholderia
#20
April S Gislason, Keith Turner, Mike Domaratzki, Silvia T Cardona
Burkholderia cenocepacia K56-2 belongs to the Burkholderia cepacia complex, a group of Gram-negative opportunistic pathogens that have large and dynamic genomes. In this work, we identified the essential genome of B. cenocepacia K56-2 using high-density transposon mutagenesis and insertion site sequencing (Tn-seq circle). We constructed a library of one million transposon mutants and identified the transposon insertions at an average of one insertion per 27 bp. The probability of gene essentiality was determined by comparing of the insertion density per gene with the variance of neutral datasets generated by Monte Carlo simulations...
November 2017: Microbial Genomics
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