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https://www.readbyqxmd.com/read/28630202/identifying-spectra-of-activity-and-therapeutic-niches-for-ceftazidime-avibactam-and-imipenem-relebactam-against-carbapenem-resistant-enterobacteriaceae
#1
Ghady Haidar, Cornelius J Clancy, Liang Chen, Palash Samanta, Ryan K Shields, Barry N Kreiswirth, M Hong Nguyen
We determined imipenem, imipenem-relebactam, ceftazidime and ceftazidime-avibactam minimum inhibitory concentrations (MICs) against 100 CRE isolates that underwent whole genome sequencing. KPCs were the most common carbapenemases. Forty-six isolates carried ESBLs. With the addition of relebactam, imipenem susceptibility increased from 8% to 88%. With the addition of avibactam, ceftazidime susceptibility increased from 0% to 85%. Neither imipenem-relebactam nor ceftazidime-avibactam was active against MBL-producers...
June 19, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28630162/real-time-dynamics-of-carbon-nanotube-porins-in-supported-lipid-membranes-visualized-by-high-speed-atomic-force-microscopy
#2
Yuliang Zhang, Ramya H Tunuguntla, Pyung-On Choi, Aleksandr Noy
In-plane mobility of proteins in lipid membranes is one of the fundamental mechanisms supporting biological functionality. Here we use high-speed atomic force microscopy (HS-AFM) to show that a novel type of biomimetic channel-carbon nanotube porins (CNTPs)-is also laterally mobile in supported lipid membranes, mimicking biological protein behaviour. HS-AFM can capture real-time dynamics of CNTP motion in the supported lipid bilayer membrane, build long-term trajectories of the CNTP motion and determine the diffusion coefficients associated with this motion...
August 5, 2017: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
https://www.readbyqxmd.com/read/28591829/benzoate-transport-in-pseudomonas-putida-csv86
#3
Alpa Choudhary, Hemant Purohit, Prashant S Phale
Pseudomonas putida strain CSV86 metabolizes variety of aromatic compounds as the sole carbon source. Genome analysis revealed the presence of genes encoding putative transporters for benzoate, p-hydroxybenzoate, phenylacetate, p-hydroxyphenylacetate and vanillate. Bioinformatic analysis revealed that benzoate transport and metabolism genes are clustered at the ben locus as benK-catA-benE-benF. Protein topology prediction suggests that BenK (aromatic acid-H+ symporter of major facilitator superfamily) has 12 transmembrane α-helices with the conserved motif, LADRXGRKX in loop 2 while BenE (benzoate-H+ symporter protein) has 11 predicted transmembrane α-helices...
June 7, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28576703/porin-flexibility-in-providencia-stuartii-cell-surface-exposed-loops-l5-and-l7-are-markers-of-providencia-porin-omppst1
#4
Que-Tien Tran, Laure Maigre, Tommaso D'Agostino, Matteo Ceccarelli, Mathias Winterhalter, Jean-Marie Pagès, Anne Davin-Regli
Epidemiologically unrelated Providencia stuartii strains isolated in hospitals in the south of France were investigated for their porin sequences and profiles. Noticeable resistance to β-lactams was found to be associated with production of extended spectrum β-lactamases or AmpC overproduction, but not metallo-β-lactamases. At the same time, the expression level of outer membrane porins was unmodified in these isolates. The identity of the amino acid sequences of the major porin OmpPst1 was less than 90% in the tested clinical strains, whereas sequences of the second major porin OmpPst2 were found to be identical in all isolates...
May 30, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28558477/mitochondrial-glutathione-regulation-and-functions
#5
Gaetano Calabrese, Bruce Morgan, Jan Riemer
SIGNIFICANCE: Mitochondrial glutathione fulfils crucial roles in a number of processes including iron-sulfur cluster biosynthesis and peroxide detoxification. Recent Advances: Genetically-encoded fluorescent probes for the glutathione redox potential (EGSH) have permitted extensive new insights into the regulation of mitochondrial glutathione redox homeostasis. These probes have revealed that the glutathione pools of the mitochondrial matrix and intermembrane space (IMS) are highly reduced, similar to the cytosolic glutathione pool...
May 30, 2017: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/28493984/nasal-airway-epithelial-cell-il-6-and-fkbp51-gene-expression-and-steroid-sensitivity-in-asthmatic-children
#6
Michael Fayon, Aurelie Lacoste-Rodrigues, Pascal Barat, Jean-Christophe Helbling, Fabienne Nacka, Patrick Berger, Marie-Pierre Moisan, Jean-Benoit Corcuff
BACKGROUND: Many asthmatic patients exhibit uncontrolled asthma despite high-dose inhaled corticosteroids (ICS). Airway epithelial cells (AEC) have distinct activation profiles that can influence ICS response. OBJECTIVES: A pilot study to identify gene expression markers of AEC dysfunction and markers of corticosteroid sensitivity in asthmatic and non-asthmatic control children, for comparison with published reports in adults. METHODS: AEC were obtained by nasal brushings and primary submerged cultures, and incubated in control conditions or in the presence of 10 ng/ml TNFalpha, 10-8M dexamethasone, or both...
2017: PloS One
https://www.readbyqxmd.com/read/28487704/a-combined-comparative-transcriptomic-metabolomic-and-anatomical-analyses-of-two-key-domestication-traits-pod-dehiscence-and-seed-dormancy-in-pea-pisum-sp
#7
Iveta Hradilová, Oldřich Trněný, Markéta Válková, Monika Cechová, Anna Janská, Lenka Prokešová, Khan Aamir, Nicolas Krezdorn, Björn Rotter, Peter Winter, Rajeev K Varshney, Aleš Soukup, Petr Bednář, Pavel Hanáček, Petr Smýkal
The origin of the agriculture was one of the turning points in human history, and a central part of this was the evolution of new plant forms, domesticated crops. Seed dispersal and germination are two key traits which have been selected to facilitate cultivation and harvesting of crops. The objective of this study was to analyze anatomical structure of seed coat and pod, identify metabolic compounds associated with water-impermeable seed coat and differentially expressed genes involved in pea seed dormancy and pod dehiscence...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28485920/bacterial-outer-membrane-porins-as-electrostatic-nanosieves-exploring-transport-rules-of-small-polar-molecules
#8
Harsha Bajaj, Silvia Acosta Gutierrez, Igor Bodrenko, Giuliano Malloci, Mariano Andrea Scorciapino, Mathias Winterhalter, Matteo Ceccarelli
Transport of molecules through biological membranes is a fundamental process in biology, facilitated by selective channels and general pores. The architecture of some outer membrane pores in Gram-negative bacteria, common to other eukaryotic pores, suggests them as prototypes of electrostatically regulated nanosieve devices. In this study, we sensed the internal electrostatics of the two most abundant outer membrane channels of Escherichia coli, using norfloxacin as a dipolar probe in single molecule electrophysiology...
May 12, 2017: ACS Nano
https://www.readbyqxmd.com/read/28481953/molecular-epidemiological-survey-of-bacteremia-by-multidrug-resistant-pseudomonas-aeruginosa-the-relevance-of-intrinsic-resistance-mechanisms
#9
Raquel Cristina Cavalcanti Dantas, Rebecca Tavares E Silva, Melina Lorraine Ferreira, Iara Rossi Gonçalves, Bruna Fuga Araújo, Paola Amaral de Campos, Sabrina Royer, Deivid William da Fonseca Batistão, Paulo Pinto Gontijo-Filho, Rosineide Marques Ribas
The bacterial factors associated with bacteremia by multidrug-resistant and extensively drug-resistant P. aeruginosa, including overexpression of efflux pumps, AmpC overproduction, and loss/alteration of the OprD porin in isolates that are non-Metallo-β-Lactamase producing were analyzed in a retrospective study. Molecular analyses included strain typing by Pulsed Field Gel Electrophoresis and identification of key genes via qualitative and quantitative PCR-based assays. Previous use of carbapenems and tracheostomy was independently associated with the development of bacteremia by extensively drug-resistant and multidrug-resistant strains of P...
2017: PloS One
https://www.readbyqxmd.com/read/28458485/phenotypic-detection-of-extended-spectrum-beta-lactamase-and-carbapenemase-co-producing-clinical-isolates-from-two-tertiary-hospitals-in-kano-north-west-nigeria
#10
Yusuf Ibrahim, Yahaya Sani, Qabli Saleh, Algarni Saleh, Gbadamosi Hakeem
BACKGROUND: Continue rise in unprofessional use of antibiotics in our hospitals and communities is worrisome. A research study was therefore conducted to detect extended spectrum beta-lactamase (ESBL), carbapenemase, metallobeta lactamase and their co-production phenotypically from isolates obtained from patients admitted to or attending two tertiary hospitals in Kano, Nigeria. METHOD: A total of 248 isolates of Escherichia coli and Klebsiella pneumoniaewere screened phenotypically for ESBL production and carbapenemase production according to CLS1 2012 breakpoints using double disk synergy test and modified Hodge test (MHT) respectively...
January 2017: Ethiopian Journal of Health Sciences
https://www.readbyqxmd.com/read/28457773/effective-assessment-of-psychotropic-medication-side-effects-using-psylog-mobile-application
#11
Martina Rojnic Kuzman, Olivier Andlauer, Kai Burmeister, Boris Dvoracek, Rebekka Lencer, Katja Koelkebeck, Nadja P Maric, Alexander Nawka, Maja Pantovic-Stefanovic, Florian Riese, Branka Aukst Margetic, Dina Bosnjak, Mirela Celic Ruzic, Marko Curkovic, Jasmina Grubsin, Zoran Madzarac, Porin Makaric, Daniela Petric, Kresimir Radic, Aleksandar Savic
No abstract text is available yet for this article.
April 27, 2017: Schizophrenia Research
https://www.readbyqxmd.com/read/28456381/high-prevalence-and-moderate-diversity-of-pseudomonas-aeruginosa-in-the-u-bends-of-high-risk-units-in-hospital
#12
Amélie Varin, Benoît Valot, Pascal Cholley, Camille Morel, Michelle Thouverez, Didier Hocquet, Xavier Bertrand
The presence of P. aeruginosa in water supply is clearly identified as a risk factor for P. aeruginosa infection in critical care units, even if routes of transmission are often unclear and remain a matter of debate. We determined here the frequency of U-bends contaminated with P. aeruginosa in high-risk units and described the population structure of this opportunistic pathogen in a non-outbreak situation. Eighty-seven U-bends from sinks of rooms in five wards were sampled 3 times and P. aeruginosa was detected in 121 of the 261 (46...
April 22, 2017: International Journal of Hygiene and Environmental Health
https://www.readbyqxmd.com/read/28453834/overproduction-of-outer-membrane-protein-a-by-acinetobacter-baumannii-as-a-risk-factor-for-nosocomial-pneumonia-bacteremia-and-mortality-rate-increase
#13
Viviana Sánchez-Encinales, Rocío Álvarez-Marín, María Eugenia Pachón-Ibáñez, Felipe Fernández-Cuenca, Alvaro Pascual, José Garnacho-Montero, Luis Martínez-Martínez, Jordi Vila, María Mar Tomás, José Miguel Cisneros, Germán Bou, Jesús Rodríguez-Baño, Jerónimo Pachón, Younes Smani
Background.: Outer membrane protein A (OmpA) is a porin involved in Acinetobacter baumannii pathogenesis. However, OmpA clinical implication in hospital-acquired infections remains unknown. We aimed to determine whether OmpA overproduction was a risk factor associated with pneumonia, bacteremia, and mortality. Methods.: We analyzed demographic, microbiological, and clinical data from 100 patients included in a unicenter cohort and 246 included in a unicenter cohort and a multicenter cohort...
March 15, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28437232/epidemiological-physiological-and-molecular-characteristics-of-a-brazilian-collection-of-carbapenem-resistant-acinetobacter-baumannii-and-pseudomonas-aeruginosa
#14
Vanessa Cordeiro Dias, Juliana Alves Resende, André Netto Bastos, Lucas Quinet De Andrade Bastos, Victor Quinet De Andrade Bastos, Ricardo Villela Bastos, Cláudio Galuppo Diniz, Vânia Lúcia Da Silva
Nonfermenting Gram-negative bacteria such as Pseudomonas aeruginosa and Acinetobacter baumannii are widespread in the environment and are increasingly associated with nosocomial infections, often associated with multidrug-resistance phenotypes. This study aimed to evaluate epidemiological, physiological, and molecular characteristics of carbapenem resistance in P. aeruginosa and A. baumannii. In total, 63 nonreplicated strains (44 A. baumannii and 19 P. aeruginosa) were isolated from hospitalized patients. Antimicrobial resistance patterns, biocide tolerance, oxidative stress, hemolytic activity, and biofilm formation were assessed...
February 24, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28414919/functional-characterization-of-an-n-terminally-truncated-mitochondrial-porin-expressed-in-neurospora-crassa
#15
Sabbir R Shuvo, Uliana Kovaltchouk, Abdullah Zubaer, Ayush Kumar, William A T Summers, Lynda J Donald, Georg Hausner, Deborah A Court
Mitochondrial porin, which forms voltage-dependent anion-selective channels (VDAC) in the outer membrane, can be folded into a 19-β-stranded barrel. The N terminus of the protein is external to the barrel and contains α-helical structure. Targeted modifications of the N-terminal region have been assessed in artificial membranes, leading to different models for gating in vitro. However, the in vivo requirements for gating and the N-terminal segment of porin are less well-understood. Using Neurospora crassa porin as a model, the effects of a partial deletion of the N-terminal segment were investigated...
April 17, 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/28413638/serologic-testing-of-a-panel-of-five-antibodies-in-inflammatory-bowel-diseases-diagnostic-value-and-correlation-with-disease-phenotype
#16
Zhi-Zhi Wang, Ke Shi, Jie Peng
The aim of the present study was to evaluate the diagnostic value of five serological antibodies, perinuclear antineutrophil cytoplasmic antibody (pANCA), anti-Saccharomyces cerevisiae antibodies [ASCA; ASCA-immunoglobulin (IgG)and ASCA-IgA], Escherichia coli outer membrane porin C antibody (anti-OmpC) and CBir1 flagellin antibody for detection in inflammatory bowel diseases. Whether the antibody status correlated with the disease phenotype was also evaluated. Sera from 71 patients with Crohn's disease (CD), 41 patients with ulcerative colitis (UC), 78 patients with other gastrointestinal diseases and 31 healthy control subjects were investigated...
April 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28412516/switching-between-nitrogen-and-glucose-limitation-unraveling-transcriptional-dynamics-in-escherichia-coli
#17
Michael Löffler, Joana Danica Simen, Jan Müller, Günter Jäger, Salaheddine Laghrami, Karin Schäferhoff, Andreas Freund, Ralf Takors
Transcriptional control under nitrogen and carbon-limitation conditions have been well analyzed for Escherichia coli. However, the transcriptional dynamics that underlie the shift in regulatory programs from nitrogen to carbon limitation is not well studied. In the present study, cells were cultivated at steady state under nitrogen (ammonia)-limited conditions then shifted to carbon (glucose) limitation to monitor changes in transcriptional dynamics. Nitrogen limitation was found to be dominated by sigma 54 (RpoN) and sigma 38 (RpoS), whereas the "housekeeping" sigma factor 70 (RpoD) and sigma 38 regulate cellular status under glucose limitation...
April 13, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28402509/identification-of-is1r-and-is10r-elements-inserted-into-ompk36-porin-gene-of-two-multidrug-resistant-klebsiella-pneumoniae-hospital-strains
#18
Anastasia I Lev, Evgeny I Astashkin, Rima Z Shaikhutdinova, Mikhail E Platonov, Nikolay N Kartsev, Nikolay V Volozhantsev, Olga N Ershova, Edward A Svetoch, Nadezhda K Fursova
Hospital Klebsiella pneumoniae strains (n = 196) were collected in 2012-16 from the patients of a Moscow neurosurgical intensive care unit. Klebsiella pneumoniae strains were multidrug-resistant and carried beta-lactamase genes blaSHV (97.4% of strains), blaCTX-M (84.7%), blaTEM (56.1%), blaOXA-48-like (49.0%) and blaNDM-1 (one strain), class 1 integrons (43.4% of strains) and porin protein ompK36 gene (100% of strains). The ompK36 porin protein gene disruption by insertion sequence (IS) elements and OmpK36 production loss in two strains were detected in this study...
May 1, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28400767/the-periplasmic-chaperone-network-of-campylobacter-jejuni-evidence-that-salc-cj1289-and-ppid-cj0694-are-involved-in-maintaining-outer-membrane-integrity
#19
Aidan J Taylor, Shadi A I Zakai, David J Kelly
The outer membrane (OM) of Gram-negative pathogenic bacteria is a key structure in host-pathogen interactions that contains a plethora of proteins, performing a range of functions including adhesion, nutrient uptake, export of effectors and interaction with innate and adaptive components of the immune system. In addition, the OM can exclude drugs and thus contribute to antimicrobial resistance. The OM of the food-borne pathogen Campylobacter jejuni contains porins, adhesins and other virulence factors that must be specifically localized to this membrane, but the protein sorting mechanisms involved are only partially understood...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28398866/genome-wide-survey-of-genes-under-positive-selection-in-avian-pathogenic-escherichia-coli-strains
#20
Thaís Cabrera Galvão Rojas, Francisco Pereira Lobo, Jorge Augusto Hongo, Renato Vicentini, Renu Verma, Renato Pariz Maluta, Wanderley Dias da Silveira
The ability to obtain bacterial genomes from the same host has allowed for comparative studies that help in the understanding of the molecular evolution of specific pathotypes. Avian pathogenic Escherichia coli (APEC) is a group of extraintestinal strains responsible for causing colibacillosis in birds. APEC is also suggested to possess a role as a zoonotic agent. Despite its importance, APEC pathogenesis still has several cryptic pathogenic processes that need to be better understood. In this work, a genome-wide survey of eight APEC strains for genes with evidence of recombination revealed that ∼14% of the homologous groups evaluated present signs of recombination...
May 2017: Foodborne Pathogens and Disease
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