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https://www.readbyqxmd.com/read/28061803/evaluation-of-mlva-for-epidemiological-typing-and-outbreak-detection-of-esbl-producing-escherichia-coli-in-sweden
#1
Lisa Helldal, Nahid Karami, Christina Welinder-Olsson, Edward R B Moore, Christina Åhren
BACKGROUND: To identify the spread of nosocomial infections and halt outbreak development caused by Escherichia coli that carry multiple antibiotic resistance factors, such as extended-spectrum beta-lactamases (ESBLs) and carbapenemases, is becoming demanding challenges due to the rapid global increase and constant and increasing influx of these bacteria from the community to the hospital setting. Our aim was to assess a reliable and rapid typing protocol for ESBL-E. coli, with the primary focus to screen for possible clonal relatedness between isolates...
January 6, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/27984780/high-rates-of-ctx-m-15-producing-escherichia-coli-and-klebsiella-pneumoniae-in-wild-boars-and-barbary-macaques-in-algeria
#2
Taous Bachiri, Sofiane Bakour, Rabia Ladjouzi, Leangapichart Thongpan, Jean Marc Rolain, Abdelaziz Touati
OBJECTIVES: The present study aimed to screen for the presence of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae in wild boars and Barbary macaques in Béjaïa and Jijel, Algeria. METHODS: A total of 216 faecal samples collected between September 2014 and August 2015 were cultured on MacConkey agar supplemented with 1μg/mL ceftazidime. Isolates were identified by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/MS)...
December 10, 2016: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/27973988/characterisation-of-extended-spectrum-%C3%AE-lactamase-and-ampc-%C3%AE-lactamase-producing-enterobacteriaceae-isolated-from-companion-animals-in-new-zealand
#3
A Karkaba, A Grinberg, J Benschop, E Pleydell
AIMS: To assess the occurrence of, and characterise, extended-spectrum-β-lactamase (ESBL) and AmpC-β-lactamase (AmpC)-producing Enterobacteriaceae isolated by veterinary diagnostic laboratories from infection sites in companion animals in New Zealand. METHODS: Selected Enterobacteriaceae isolates were submitted by seven New Zealand veterinary diagnostic laboratories. They were isolated from infection sites in companion animals between June 2012 and June 2013, and were resistant to amoxicillin-clavulanic acid, fluoroquinolones, or any combination of two or more antimicrobials...
December 14, 2016: New Zealand Veterinary Journal
https://www.readbyqxmd.com/read/27855067/tigecycline-non-susceptibility-occurs-exclusively-in-fluoroquinolone-resistant-escherichia-coli-clinical-isolates-including-the-major-multidrug-resistant-lineages-o25b-h4-st131-h30r-and-o1-st648
#4
Toyotaka Sato, Yuuki Suzuki, Tsukasa Shiraishi, Hiroyuki Honda, Masaaki Shinagawa, Soh Yamamoto, Noriko Ogasawara, Hiroki Takahashi, Satoshi Takahashi, Yutaka Tamura, Shin-Ichi Yokota
Tigecycline (TGC) is a last-line drug for multidrug-resistant Enterobacteriaceae. We investigated the mechanism(s) underlying TGC non-susceptibility (TGC-resistance/intermediate) in Escherichia coli clinical isolates. The MIC of TGC was determined for 277 fluoroquinolone-susceptible isolates (ciprofloxacin [CIP] MIC: <0.125 mg/L, and 194 fluoroquinolone-resistant isolates (CIP MIC: >2 mg/L). The MIC50 and MIC90 for TGC in fluoroquinolone-resistant isolates were 2-fold higher than those in fluoroquinolone-susceptible isolates (MIC50: 0...
November 14, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27666313/phylogenetic-diversity-antimicrobial-susceptibility-and-virulence-characteristics-of-phylogroup-f-escherichia-coli-in-australia
#5
Belinda Vangchhia, Sam Abraham, Jan M Bell, Peter Collignon, Justine S Gibson, Paul R Ingram, James R Johnson, Karina Kennedy, Darren J Trott, John D Turnidge, David M Gordon
Unlike Escherichia coli strains belonging to phylogroup B2, the clinical significance of strains belonging to phylogroup F is not well understood. Here we report on a collection of phylogroup F strains recovered in Australia from faeces and extra-intestinal sites from humans, companion animals and native animals, as well as from poultry meat and water samples. The distribution of sequence types was clearly non-random with respect to isolate source. The antimicrobial resistance and virulence trait profiles also varied with the sequence type of the isolate...
November 2016: Microbiology
https://www.readbyqxmd.com/read/27566913/plasmid-mediated-colistin-resistance-in-escherichia-coli-from-the-arabian-peninsula
#6
Ágnes Sonnevend, Akela Ghazawi, Manaf Alqahtani, Atef Shibl, Wafa Jamal, Rayhan Hashmey, Tibor Pal
OBJECTIVES: Searching for the presence of the mcr-1 gene in colistin resistant Enterobacteriaceae in countries of the Arabian Peninsula. METHODS: Seventy-five independent, colistin resistant Enterobacteriaceae strains isolated from clinical cases in Bahrain, Kuwait, Oman, Saudi Arabia and the United Arab Emirates were tested by PCR for the mcr-1 gene. mcr-1 positive strains were genotyped, and their antibiotic susceptibility was established. The mcr-1 containing plasmids were mobilized into Escherichia coli K-12 and their sequence was determined...
September 2016: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/27550364/emergence-of-ndm-5-and-mcr-1-producing-escherichia-coli-clones-st648-and-st156-from-a-single-muscovy-duck-cairina-moschata
#7
Run-Shi Yang, Youjun Feng, Xiao-Yue Lv, Jia-Hong Duan, Jing Chen, Liang-Xing Fang, Jing Xia, Xiao-Ping Liao, Jian Sun, Ya-Hong Liu
Two Escherichia coli clones (sequence type 648 [ST648] and ST156) that coproduce NDM-5 and MCR-1 were detected from a single fowl in China. The blaNDM-5 gene was found on the two indistinguishable IncX3 plasmids from the two different E. coli isolates, whereas the mcr-1 gene was located on IncHI2 and IncI2 plasmids, respectively, suggesting that blaNDM-5 and mcr-1 have spread in avian intestinal flora. Also, the two strains harbor blaTEM-1, blaCTX-M-55, fosA3, and aac(6')-Ib The multiresistant E. coli strains (especially the epidemic clone ST648) might raise a potential threat to human health via food chain transmission...
November 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27530830/multidrug-resistance-dissemination-by-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-causing-community-acquired-urinary-tract-infection-in-the-central-western-region-brazil
#8
Laura Fernandes Gonçalves, Paulo de Oliveira Martins-Júnior, Ana Beatriz Fabrício de Melo, Rafaella Christina Rocha Moreira da Silva, Vicente de Paulo Martins, André Pitondo-Silva, Tatiana Amabile de Campos
The aim of this work was to analyse extended-spectrum β-lactamase (ESBL)-producing Escherichia coli strains isolated from outpatients with signs of cystitis in Hospital Universitário de Brasília (Brasília, Brazil) during the period July 2013 to April 2014. E. coli isolated from urine culture were identified and their antibiotic susceptibility profile was determined by VITEK 2. ESBL-producing strains identified were submitted to PCR for Clermont phylotyping, CTX-M group typing and virulence determinant detection, and clonal relationships were determined by enterobacterial repetitive intergenic consensus (ERIC)-PCR...
September 2016: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/27462301/occurrence-of-oxa-48-carbapenemase-and-other-%C3%AE-lactamase-genes-in-esbl-producing-multidrug-resistant-escherichia-coli-from-dogs-and-cats-in-the-united-states-2009-2013
#9
Xiaoqiang Liu, Kamoltip Thungrat, Dawn M Boothe
OBJECTIVE: The aim of this study was to explore the occurrence and molecular characterization of extended-spectrum β-lactamases (ESBL), plasmid-mediated AmpC β-lactamase (pAmpC) and carbapenemases among ESBL-producing multidrug resistant (MDR) Escherichia coli from dogs and cats in the United States. METHODS: Of 2443 E.coli isolated from dogs and cats collected between August 2009 and January 2013, 68 isolates were confirmed as ESBL-producing MDR ones. PCR and sequencing were performed to identify β-lactamases and plasmid-mediated quinolone resistance (PMQR) genes, and shed light on the virulence gene profiles, phylogenetic groups and ST types...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27358749/genomic-insights-into-the-esbl-and-mcr-1-producing-st648-escherichia-coli-with-multi-drug-resistance
#10
Huimin Zhang, Christopher H Seward, Zuowei Wu, Huiyan Ye, Youjun Feng
Polymyxin acts as an ultimate line of refuge against the severe infections by multidrug-resistant Gram-negative pathogens. This conventional idea is challenged dramatically by the recent discovery of mobile colistin resistance gene (mcr-1) is prevalent in food animals and human beings worldwide. More importantly, the mcr-1 gene was found to be co-localized with other antibiotic resistance genes, raising the possibility that super-bugs with pan-drug resistance are emerging. However, little is reported on the genomes of the mcr-1-positive bacterial host reservoirs...
2016: Science Bulletin
https://www.readbyqxmd.com/read/27014251/carriage-of-extended-spectrum-beta-lactamase-plasmids-does-not-reduce-fitness-but-enhances-virulence-in-some-strains-of-pandemic-e-coli-lineages
#11
Katharina Schaufler, Torsten Semmler, Derek J Pickard, María de Toro, Fernando de la Cruz, Lothar H Wieler, Christa Ewers, Sebastian Guenther
Pathogenic ESBL-producing E. coli lineages occur frequently worldwide, not only in a human health context but in animals and the environment, also in settings with low antimicrobial pressures. This study investigated the fitness costs of ESBL-plasmids and their influence on chromosomally encoded features associated with virulence, such as those involved in the planktonic and sessile behaviors of ST131 and ST648 E. coli. ESBL-plasmid-carrying wild-type E. coli strains, their corresponding ESBL-plasmid-"cured" variants (PCV), and complementary ESBL-carrying transformants were comparatively analyzed using growth curves, Omnilog® phenotype microarray (PM) assays, macrocolony and biofilm formation, swimming motility, and RNA sequence analysis...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27007258/fecal-carriage-of-extended-spectrum-%C3%AE-lactamases-in-healthy-humans-poultry-and-wild-birds-in-le%C3%A3-n-nicaragua-a-shared-pool-of-blactx-m-genes-and-possible-interspecies-clonal-spread-of-extended-spectrum-%C3%AE-lactamases-producing-escherichia-coli
#12
Badrul Hasan, Karl Laurell, Mufti Mahmud Rakib, Erik Ahlstedt, Jorge Hernandez, Mercedes Caceres, Josef D Järhult
Antibiotic-resistant bacteria are a major concern in the healthcare of today, especially the increasing number of gram-negative bacteria producing β-lactamases such as extended-spectrum β-lactamases (ESBLs). However, little is known about the relationship of ESBL producers in humans and domestic and wild birds, especially in a low-income setting. Therefore, we studied the fecal carriage of ESBL-producing Escherichia coli and Klebsiella pneumoniae in healthy humans, poultry, and wild birds in the vicinity of León, Nicaragua...
December 2016: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/26746714/long-term-colonization-by-bla-ctx-m-harboring-escherichia-coli-in-healthy-japanese-people-engaged-in-food-handling
#13
Kunihiko Nakane, Kumiko Kawamura, Kensuke Goto, Yoshichika Arakawa
The actual state of intestinal long-term colonization by extended-spectrum β-lactamase (ESBL)-producing Escherichia coli in healthy Japanese people remains unclear. Therefore, a total of 4,314 fecal samples were collected from 2,563 food handlers from January 2010 to December 2011. Approximately 0.1 g of each fecal sample was inoculated onto a MacConkey agar plate containing cefotaxime (1 μg/ml). The bacterial colonies that grew on each plate were checked for ESBL production by the double-disk synergy test, as recommended by the Clinical and Laboratory Standards Institute...
January 8, 2016: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/26437344/distribution-of-virulence-factors-in-esbl-producing-escherichia-coli-isolated-from-the-environment-livestock-food-and-humans
#14
Andrea Müller, Roger Stephan, Magdalena Nüesch-Inderbinen
In this study, extended-spectrum ß-lactamase (ESBL)-producing Escherichia coli isolates recovered from the following sources were characterized with regard to the occurrence and distribution of uropathogenic and enteric pathogenic virulence factors: surface waters (rivers and lakes, n=60), the intestines of freshwater fish (n=33), fresh vegetables (n=26), retail poultry meat (n=13) and the fecal samples of livestock (n=28), healthy humans (n=34) and primary care patients (n=13). Among the 207 isolates, 82% tested positive by PCR for one or more of the virulence factors (VF) that predict uropathogenicity, TraT, fyuA, chuA, PAI, yfcv or vat...
January 15, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/26149956/emergence-of-kpc-2-producing-escherichia-coli-isolates-in-an-urban-river-in-harbin-china
#15
Guofeng Xu, Yue Jiang, Wei An, Hongdong Wang, Xiuying Zhang
Three KPC-2-producing Escherichia coli (E1, E2, and E3) were recovered from water samples of an urban river in the city of Harbin, China. Antimicrobial susceptibility was determined by broth microdilution. Molecular characterization and genetic relatedness of the isolates were determined by polymerase chain reaction (PCR), pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST) and PCR-directed phylotyping. Plasmids were analyzed by conjugation, S1-PFGE, Southern blotting and PCR-based replicon typing (PBRT)...
September 2015: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/26119348/increase-of-widespread-a-b1-and-d-escherichia-coli-clones-producing-a-high-diversity-of-ctx-m-types-in-a-portuguese-hospital
#16
Carla Rodrigues, Elisabete Machado, João Pires, Helena Ramos, Ângela Novais, Luísa Peixe
AIM: To characterize temporal shifts in extended-spectrum β-lactamases (ESBLs) and clones of clinical Escherichia coli isolates. MATERIALS & METHODS: All ESBL-producing E. coli isolates from a Portuguese hospital (n = 112; June 2006-June 2007 and January-December 2010) were characterized by identification of phylogenetic groups, ESBL-types and virulence genes, XbaI-PFGE and MLST. RESULTS: We observed a substantial increase in widespread E...
2015: Future Microbiology
https://www.readbyqxmd.com/read/26062812/retrospective-identification-of-a-previously-undetected-clinical-case-of-oxa-48-producing-k-pneumoniae-and-e-coli-the-importance-of-adequate-detection-guidelines
#17
Ina Willemsen, Joost van Esser, Marjolein Kluytmans-van den Bergh, Kai Zhou, John W Rossen, Carlo Verhulst, Kees Verduin, Jan Kluytmans
INTRODUCTION: The laboratory detection of OXA-48-carbapenemase-producing Enterobacteriaceae is difficult, as minimum inhibition concentrations for carbapenems are often below the clinical breakpoint. In 2011, the Dutch national guideline for the detection of highly resistant micro-organisms was issued, which includes recommendations on the use of carbapenem screening breakpoints for the detection of carbapenemase-producing Enterobacteriaceae. MATERIALS AND METHODS: During a validation study of the Check-MDR CT103 microarray (Check-Points, Wageningen, The Netherlands) in 2013, an OXA-48-like carbapenemase gen was identified in two isolates that were previously obtained from a patient with non-Hodgkin lymphoma in 2007...
February 2016: Infection
https://www.readbyqxmd.com/read/25858833/draft-genome-sequence-of-ndm-5-producing-escherichia%C3%A2-coli-sequence-type-648-and-genetic-context-of-blandm-5-in-australia
#18
Alexander M Wailan, David L Paterson, Michael Caffery, David Sowden, Hanna E Sidjabat
We report here the draft genome sequence of uropathogenic Escherichia coli sequence type 648 (ST648) possessing blaNDM-5 from a 55-year-old female in Australia with a history of travel to India. The plasmid-mediated blaNDM-5 was in a genetic context nearly identical to that of the GenBank entry of an IncX3 blaNDM-5 plasmid previously reported from India (Klebsiella pneumoniae MGR-K194).
2015: Genome Announcements
https://www.readbyqxmd.com/read/25824221/clinical-epidemiology-and-molecular-analysis-of-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-in-nepal-characteristics-of-sequence-types-131-and-648
#19
Jatan Bahadur Sherchan, Kayoko Hayakawa, Tohru Miyoshi-Akiyama, Norio Ohmagari, Teruo Kirikae, Maki Nagamatsu, Masayoshi Tojo, Hiroshi Ohara, Jeevan B Sherchand, Sarmila Tandukar
Recently, CTX-M-type extended-spectrum-β-lactamase (ESBL)-producing Escherichia coli strains have emerged worldwide. In particular, E. coli with O antigen type 25 (O25) and sequence type 131 (ST131), which is often associated with the CTX-M-15 ESBL, has been increasingly reported globally; however, epidemiology reports on ESBL-producing E. coli in Asia are limited. Patients with clinical isolates of ESBL-producing E. coli in the Tribhuvan University teaching hospital in Kathmandu, Nepal, were included in this study...
2015: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/25712794/drug-susceptibility-and-molecular-epidemiology-of-escherichia-coli-in-bloodstream-infections-in-shanghai-china-2011-2013
#20
Sheng-Yuan Zhao, Yan-Chun Wang, Shu-Zhen Xiao, Xiao-Fei Jiang, Xiao-Kui Guo, Yu-Xing Ni, Li-Zhong Han
BACKGROUND: Prevention and management of Escherichia coli bloodstream infections (EC-BSIs) have become increasingly complicated by antimicrobial resistance and rapid dissemination. We investigated the antimicrobial epidemiology and phylogenetic background of clinical E. coli isolates from patients with bloodstream infections in Shanghai from 2011 to 2013. METHODS: Escherichia coli isolates causing bloodstream infections were consecutively collected between June 2011 and June 2013...
May 2015: Infectious Diseases
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