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Ryan R Lenz, Wenhao Dai
Phytoplasmas such as "Candidatus Phytoplasma pruni," the causal agent of X-disease of stone fruits, lack detailed biological analysis. This has limited the understanding of plant resistance mechanisms. Chokecherry (Prunus virginiana L.) is a promising model to be used for the plant-phytoplasma interaction due to its documented ability to resist X-disease infection. A consensus chokecherry genetic map "Cho" was developed with JoinMap 4.0 by joining two parental maps. The new map contains a complete set of 16 linkage groups, spanning a genetic distance of 2,172 cM with an average marker density of 3...
2017: Frontiers in Plant Science
Mengya Shi, Xiao Hu, Yu Wei, Xu Hou, Xue Yuan, Jun Liu, Yueping Liu
Auxin has long been known as a critical phytohormone that regulates fruit development in plants. However, due to the lack of an enlarged ovary wall in the model plants Arabidopsis and rice, the molecular regulatory mechanisms of fruit division and enlargement remain unclear. In this study, we performed small RNA sequencing and degradome sequencing analyses to systematically explore post-transcriptional regulation in the mesocarp at the hard core stage following treatment of the peach (Prunus persica L.) fruit with the synthetic auxin α-naphthylacetic acid (NAA)...
December 13, 2017: International Journal of Molecular Sciences
Hongchun Shen, Honglian Wang, Li Wang, Lu Wang, Menglian Zhu, Yao Ming, Sha Zhao, Junming Fan, En Yin Lai
Liver cancer is the second most lethal cancer and hepatocellular carcinoma (HCC) is the primary cancer subgroup. However, the current chemotherapy agents remain ineffective and present wide side effects for advanced HCC patient. In this study, we investigated the antitumor role of ethanol extract of root of peach tree (Prunus persica (L.) Batsch and hereafter designated as TSG in short of its Chinese name), which is an important ingredient in Chinese medicine prescription, in liver cancer cell HepG2. By cell viability assay, we showed that addition of TSG in the culture medium inhibited the cell growth of HepG2 cells in a dose and time-dependent way...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
Shaan Bibi Jaffri, Khuram Shahzad Ahmad
The present study has demystified the first and single step prunosynthesis of the spherical silver nanoparticles (AgNPs) from aqueous fruit extract of angiospermic plant, Prunus cerasifera, which has remarkable fusion of reducing cum stabilizing bioactive components (phenols, anthocyanins, carotenoids, flavonoids, organic acids, tannins and vitamins). Highly stable prunosynthetic AgNPs with 2.04 nm average crystallite size were synthesized in dark and in sunlight at optimized condition of temperature, time and P...
December 11, 2017: Artificial Cells, Nanomedicine, and Biotechnology
Marco Cirilli, Tiziano Flati, Silvia Gioiosa, Ilario Tagliaferri, Angelo Ciacciulli, Zhongshan Gao, Stefano Gattolin, Filippo Geuna, Francesco Maggi, Paolo Bottoni, Laura Rossini, Daniele Bassi, Tiziana Castrignanò, Giovanni Chillemi
Applying next-generation sequencing (NGS) technologies to species of agricultural interest has the potential to accelerate the understanding and exploration of genetic resources. The storage, availability and maintenance of huge quantities of NGS-generated data remains a major challenge. The PeachVar-DB portal, available at, is an open-source catalogue of genetic variants present in peach (P. persica L. Batsch) and wild-related species of Prunus genera, annotated from 146 samples publicly released on the Sequence Reads Archive (SRA)...
December 4, 2017: Plant & Cell Physiology
Chunxian Chen, Clive H Bock, Bruce W Wood
Venturia carpophila causes peach scab, a disease that renders peach (Prunus persica) fruit unmarketable. We report a high-quality draft genome sequence (36.9 Mb) of V. carpophila from an isolate collected from a peach tree in central Georgia in the United States. The genome annotation is described and a phylogenetic analysis of the pathogen is presented. The genome sequence will be a useful resource for various studies on the pathogen, including the biology and ecology, taxonomy and phylogeny, host interaction and coevolution, isolation and characterization of genes of interest, and development of molecular markers for genotyping and mapping...
2017: Standards in Genomic Sciences
Sarahí Rodríguez-González, Iza F Pérez-Ramírez, Eduardo Castaño-Tostado, Silvia Amaya-Llano, Mario E Rodríguez-García, Rosalía Reynoso-Camacho
BACKGROUND: This study aimed to concentrate dietary fiber (DF) from peach (Prunus persica) juice by-product (PJBP), to improve its functional properties, and its polyphenols bioavailability. The dietary fiber concentrates (DFC) were obtained from PJBP using water/ethanol treatments (100:0, 20:80, 50:50, 80:20, and 0:100, v/v) at 1:5 ratio (wet weight/solvent, w/v) for 5 and 20 min at 21 °C. RESULTS: All treatments concentrated condensed tannins, total and insoluble DF, founding the highest content with 100% H2O treatment...
December 6, 2017: Journal of the Science of Food and Agriculture
Zhe Wang, Yanfei Zeng, Zhendong Zhang, Songbai Sheng, Ju Tian, Rongling Wu, Xiaoming Pang
There is evidence that a band of dry climate separated plants in East Asia into distinct northern and southern groups. However, few studies have focused on the arid belt in this region, especially with regard to plants. We analyzed genetic variation in 22 populations of Siberian apricot (Prunus sibirica L.), a temperate deciduous species distributed in this arid belt, using two chloroplast DNA (cpDNA) sequences, seven chloroplast microsatellite loci (cpSSRs), and 31 nuclear microsatellite loci (nSSRs), to study its phylogeography...
2017: Frontiers in Plant Science
Michael J Walker, Malcolm Burns, Milena Quaglia, Gavin Nixon, Christopher J Hopley, Kirstin M Gray, Victoria Moore, Malvinder Singh, Simon Cowen
It is now well known that an incident investigated in the United Kingdom in 2015 of cumin alleged to be contaminated with almond, a risk for people with almond allergy, was caused by the Prunus species, Prunus mahaleb. In the United Kingdom, the Government Chemist offers a route of technical appeal from official findings in the food control system. Findings of almond in two official samples, cumin and paprika, which had prompted action to exclude the consignments from the food chain, were so referred. Herein are described the approaches deployed to resolve the analytical issues during the investigation of the incidents...
December 5, 2017: Journal of AOAC International
Shaoxuan Li, Zhanru Shao, Xiling Fu, Wei Xiao, Ling Li, Ming Chen, Mingyue Sun, Dongmei Li, Dongsheng Gao
BACKGROUND: MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression of target mRNAs involved in plant growth, development, and abiotic stress. As one of the most important model plants, peach (Prunus persica) has high agricultural significance and nutritional values. It is well adapted to be cultivated in greenhouse in which some auxiliary conditions like temperature, humidity, and UVB etc. are needed to ensure the fruit quality. However, little is known about the genomic information of P...
December 2, 2017: BMC Genomics
Priyanka Singh, Sungeun Ahn, Jong-Pyo Kang, Soshnikova Veronika, Yue Huo, Hina Singh, Mohan Chokkaligam, Mohamed El-Agamy Farh, Verónica Castro Aceituno, Yeon Ju Kim, Deok-Chun Yang
Recently, green metal nanoparticles have received global attention owing to their economical synthesis, biocompatible nature, widespread biomedical and environmental applications. Current study demonstrates a sustainable approach for the green synthesis of silver nanoparticles (P-AgNPs) and gold nanoparticles (P-AuNPs) from P. serrulata fresh fruit extract. The silver and gold nanoparticles were synthesized in a very rapid, efficient and facile manner, within 50 min and 30 s at 80 °C, respectively. The nanoparticles were characterized by using visual observation, UV-Vis, FE-TEM, EDX, elemental mapping, FT-IR, XRD and DLS, which confirmed the formation of monodispersed, crystalline and stable nanoparticles...
November 30, 2017: Artificial Cells, Nanomedicine, and Biotechnology
Macarena Farcuh, Bosheng Li, Rosa M Rivero, Lyudmila Shlizerman, Avi Sadka, Eduardo Blumwald
We investigated sugar metabolism in leaves and fruits of two Japanese plum (Prunus salicina Lindl.) cultivars, the climacteric Santa Rosa and its bud sport mutant the non-climacteric Sweet Miriam, during development on the tree. We previously characterized differences between the two cultivars. Here, we identified key sugar metabolic pathways. Pearson coefficient correlations of metabolomics and transcriptomic data and weighted gene co-expression network analysis (WGCNA) of RNA sequencing (RNA-Seq) data allowed the identification of 11 key sugar metabolism-associated genes: sucrose synthase, sucrose phosphate synthase, cytosolic invertase, vacuolar invertase, invertase inhibitor, α-galactosidase, β-galactosidase, galactokinase, trehalase, galactinol synthase, and raffinose synthase...
November 25, 2017: Journal of Experimental Botany
Wenquan Bao, Tana Wuyun, Tiezhu Li, Huimin Liu, Zhongmao Jiang, Xuchun Zhu, Hongyan Du, Yu-E Bai
Prunus mira Koehne, an important economic fruit crop with high breeding and medicinal values, and an ancestral species of many cultivated peach species, has recently been declared an endangered species. However, basic information about genetic diversity, population structure, and morphological variation is still limited for this species. In this study, we sampled 420 P. mira individuals from 21 wild populations in the Tibet plateau to conduct a comprehensive analysis of genetic and morphological characteristics...
2017: PloS One
Daniele Bevacqua, Bénédicte Quilot-Turion, Luca Bolzoni
Brown rot, caused by Monilinia spp., is a major disease of stone fruits and, in favorable environmental conditions and in the absence of fungicide treatments, it causes important economic losses. In the present work, we propose a modification of classical susceptible, exposed, infectious and removed (SEIR) compartmental models to grasp the peculiarities of the progression of brown rot epidemics in stone fruit orchards in the last stage of the fruit growth (i.e. from the end of the pit hardening to harvest time)...
November 28, 2017: Phytopathology
Wei Song, Shao-Tong Qin, Fei-Xiang Fang, Zhen-Jiang Gao, Dan-Dan Liang, Lu-Lu Liu, Hong-Tao Tian, Hai-Bo Yang
Prunus cerasifera has a rich resource and a weak utilization rate and its biological functions have been investigated. We found that the contents of total phenol (TP) in leaves and branches of Prunus cerasifera were 117.8 ± 8.8 and 100.04 ± 0.9 mg/g, respectively; the contents of soluble condensed tannin (SCT) were 73.95 ± 0.9 and 78.65 ± 4.1 mg/g, respectively; the structure of SCT containing afzelechin/epiafzelechin, catechin/epicatechin, and atechin/epicatechin as the main units and the SCT from leaves and branches exhibited better anti-tyrosinase and antioxidant activities...
November 27, 2017: Applied Biochemistry and Biotechnology
Ya-Yun Huo, Gui-Fen Li, Yan-Hong Qiu, Wei-Min Li, Yong-Jiang Zhang
Prunus necrotic ringspot virus (PNRSV) is one of the most devastating viruses to Prunus spp. In this study, we developed a diagnostic system RT-CPA-NATSC, wherein reverse transcription-cross-priming amplification (RT-CPA) is coupled with nucleic acid test strip cassette (NATSC), a vertical flow (VF) visualization, for PNRSV detection. The RT-CPA-NATSC assay targets the encoding gene of the PNRSV coat protein with a limit of detection of 72 copies per reaction and no cross-reaction with the known Prunus pathogenic viruses and viroids, demonstrating high sensitivity and specificity...
November 23, 2017: Scientific Reports
Xiang Zhu, Kun Xiao, Haiyang Cui, Jianfang Hu
Root-knot nematodes (RKNs), particularly Meloidogyne incognita, are the most devastating soil-borne pathogens that significantly affect the production of Prunus spp. fruit. RKN infection is difficult to control and consequently causes massive yield losses each year. However, several germplasms of wild Prunus spp. have been shown to display resistance to M. incognita. Consequently, both the isolation of novel plant resistance (R) genes and the characterization of their resistance mechanisms are important strategies for future disease control...
2017: Frontiers in Microbiology
Shashi N Goonetilleke, Timothy J March, Michelle G Wirthensohn, Pere Arús, Amanda R Walker, Diane E Mather
In crop plant genetics, linkage maps provide the basis for the mapping of loci that affect important traits and for the selection of markers to be applied in crop improvement. In outcrossing species such as almond [Prunus dulcis (Mill.) D.A. Webb], application of a double pseudo-testcross mapping approach to the F1 progeny of a bi-parental cross leads to the construction of a linkage map for each parent. Here, we report on the application of genotyping-by-sequencing to discover and map single-nucleotide polymorphisms in the almond cultivars 'Nonpareil' and 'Lauranne'...
November 15, 2017: G3: Genes—Genomes—Genetics
Linsen Zhang, Xiaoni Yang, Xiangning Qi, Chunhui Guo, Zhaobin Jing
BACKGROUND: The almond tree (Prunus amygdalus Batsch) is an important nut tree grown in subtropical regions that produces nutrient-rich nuts. However, a paucity of genomic information and DNA markers has restricted the development of modern breeding technologies for almond trees. RESULTS: In this study, almonds were sequenced with Illumina paired-end sequencing technology to obtain transcriptome data and develop simple sequence repeats (SSR) markers. We generated approximately 64 million clean reads from the various tissues of mixed almonds, and a total of 42,135 unigenes with an average length of 988 bp were obtained in the present study...
2018: Hereditas
Antonio Mirto, Federica Iannuzzi, Petronia Carillo, Loredana F Ciarmiello, Pasqualina Woodrow, Amodio Fuggi
In this article, we reported the original data obtained by the study of metabolites and enzymes involved in sweet cherry antioxidant system. We measured hydrogen peroxide (H2O2) and malondialdehyde (MDA), which are indicator of oxidative stress. Moreover, we measured the concentration of reduced and oxidized ascorbate and glutathione that are involved in ROS detoxification together with phenolics, anthocyanins and tocopherols. Among antioxidant enzymes, we analyzed the activities of ascorbate peroxidase (APX; EC 1...
December 2017: Data in Brief
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