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Marker assisted selection

K M Madder, J J McKinnon, G W Torres, K G Krone, J L Duncombe, C D Luby, F C Buchanan
Previous studies have shown that the interaction between limiting vitamin A (VA) and an alcohol dehydrogenase 1 C (ADH1C) variant in beef cattle results in increased intramuscular fat in the longissimus thoracis muscle in one genotype when fed low dietary VA. Although quality grade is important for increased profitability and improving taste characteristics of beef products, limiting VA too drastically can impair animal welfare. The objectives of this study were to determine if this marker-assisted management strategy would be effective, and whether any impairment in immune function would occur in a feedlot setting...
June 18, 2018: Animal: An International Journal of Animal Bioscience
I Subhakara Rao, C N Neeraja, B Srikanth, D Subrahmanyam, K N Swamy, K Rajesh, P Vijayalakshmi, T Vishnu Kiran, N Sailaja, P Revathi, P Raghuveer Rao, L V Subba Rao, K Surekha, V Ravindra Babu, S R Voleti
With the priority of the low input sustainable rice cultivation for environment friendly agriculture, NUE of rice becomes the need of the hour. A set of 472 rice genotypes comprising landraces and breeding lines were evaluated for two seasons under field conditions with low and recommended nitrogen and >100 landraces were identified with relative higher yield under low nitrogen. Donors were identified for higher N uptake, N translocation into grains and grain yield under low N. Grains on secondary branches, N content in grain and yield appears to be the selection criterion under low N...
June 15, 2018: Scientific Reports
Nicholas J Ashton, Michael Schöll, Kerstin Heurling, Eleni Gkanatsiou, Erik Portelius, Kina Höglund, Gunnar Brinkmalm, Abdul Hye, Kaj Blennow, Henrik Zetterberg
At the center of Alzheimer's disease pathogenesis is the aberrant aggregation of amyloid-β (Aβ) into oligomers, fibrils and plaques. Effective monitoring of Aβ deposition directly in patients is essential to assist anti-Aβ therapeutics in target engagement and participant selection. In the advent of approved anti-Aβ therapeutics, biomarkers will become of fundamental importance in initiating treatments having disease modifying effects at the earliest stage. Two well-established Aβ biomarkers are widely utilized: Aβ-binding ligands for positron emission tomography and immunoassays to measure Aβ42 in cerebrospinal fluid...
June 15, 2018: Biomarkers in Medicine
Run-Li He, Yi-Li Han, Fang Wang, Ji-Quan Liu, Li-Hua Wang
In order to enrich the library of SSR and provide more powerful tools for molecular marker-assisted breeding in Astragalus membranaceus var. mongholicus, simple sequence repeats (SSR) loci in its transcriptome were searched in 18 040 unigenes (>=1 kb) by using MISA. SSR loci information was analyzed and SSR primers were designed by Primer 3. Furthermore, 110 pairs of primers were randomly selected for the polymorphic analysis on 20 plants collected from different habitats. A total of 5 640 SSRs were found in the transcriptome of A...
May 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Zhenjie Cao, Lu Wang, Yajing Xiang, Xiaocen Liu, Zhigang Tu, Yun Sun, Yongcan Zhou
The major histocompatibility complex (MHC) plays an important role in the vertebrate immune response to antigenic peptides, and it is essential for recognizing foreign pathogens in organisms. In this study, MHC class IIα (Trov-MHC IIα) from the golden pompano (Trachinotus ovatus) was first cloned and identified. The gene structure of Trov-MHC IIα was contained four exons and three introns. High levels of polymorphism were found in the exon 2 of Trov-MHC IIα. A total of 29 different MHC class IIα alleles with high polymorphism were identified from 80 individuals...
June 11, 2018: Fish & Shellfish Immunology
Celia M Cantín, Pere Arús, Iban Eduardo
OBJECTIVE: Peach brachytic dwarfism determined by Dwarf gene (Dw) is an undesired trait segregating in some peach breeding programs. Recently, a single nucleotide polymorphism (SNP) mutation in the gibberellin insensitive dwarf 1 (GID1) peach gene causing brachytic dwarfism was described. In this research we wanted to validate this marker in an F2 population of the 'Nectavantop' peach cultivar (Nv) to include it as a marker assisted selection tool for peach breeding programs. RESULTS: The observed segregation of the trait was in agreement with that of a recessive gene, the individuals homozygous for the recessive allele (dwdw) presenting the dwarf genotype...
June 14, 2018: BMC Research Notes
Xiaoran Duan, Yongli Yang, Sihua Wang, Xiaolei Feng, Tuanwei Wang, Pengpeng Wang, Wu Yao, Liuxin Cui, Wei Wang
Cholinesterase activity (ChA), the effective biomarker for organophosphate pesticide exposure, is possibly affected by single nucleotide polymorphisms (SNPs) in cell-cycle-related genes. One hundred and eighty workers with long-term exposure to omethoate and 115 healthy controls were recruited to explore the gene-gene and gene-environment interactions. The acetylthiocholine and dithio-bis-(nitrobenzoic acid) method was used to detect the cholinesterase activities in whole blood, erythrocytes and plasma. Genetic polymorphisms were determined by the PCR-RFLP and direct PCR electrophoresis methods...
May 2018: Royal Society Open Science
Mimi Xie, Yao Ming, Feng Shao, Jianbo Jian, Yaoguang Zhang, Zuogang Peng
Single-nucleotide polymorphism (SNP) markers and high-density genetic maps are important resources for marker-assisted selection, mapping of quantitative trait loci (QTLs) and genome structure analysis. Although linkage maps in certain catfish species have been obtained, high-density maps remain unavailable in the economically important southern catfish ( Silurus meridionalis ). Recently developed restriction site-associated DNA (RAD) markers have proved to be a promising tool for SNP detection and genetic map construction...
May 2018: Royal Society Open Science
Hongfang Guo, Sayed Haidar Abbas Raza, Nicola M Schreurs, Rajwali Khan, Dawei Wei, Li Wang, Song Zhang, Le Zhang, Sen Wu, Irfan Ullah, Seyed Mahdi Hosseini, Linsen Zan
Krüppel-like factor 3 (KLF3), a member of the Krüppel-like factor (KLF) family, plays an important role in adipogenesis and lipid metabolism. The aim of this study was to investigate whether KLF3 could be used as a candidate gene in the breeding of cattle. The expression pattern of bovine KLF3 gene revealed that it was highly expressed in abdominal fat and perirenal fat. Using DNA sequencing, three single nucleotide polymorphisms (SNPs) within the promoter regions of KLF3 gene were identified in 448 Qinchuan cattle, which are located in the recognition sequences of 11 transcription factors and the four haplotypes representing four potential different compositions of polymorphic potential cis-acting elements...
June 8, 2018: Gene
Jianwei Lv, Nian Liu, Jianbin Guo, Zhijun Xu, Xinping Li, Zhendong Li, Huaiyong Luo, Xiaoping Ren, Li Huang, Xiaojing Zhou, Yuning Chen, Weigang Chen, Yong Lei, Jinxing Tu, Huifang Jiang, Boshou Liao
The peanut ( Arachis hypogaea L.) is an important grain legume extensively cultivated worldwide, supplying edible oil and protein for human consumption. As in many other crops, plant height is a crucial factor in determining peanut architecture traits and has a unique effect on resistance to lodging and efficiency of mechanized harvesting as well as yield. Currently, the genetic basis underlying plant height remains unclear in peanut, which have hampered marker-assisted selection in breeding. In this study, we conducted a quantitative trait locus (QTL) analysis for peanut plant height by using two recombinant inbred line (RIL) populations including "Yuanza 9102 × Xuzhou 68-4 (YX)" and "Xuhua 13 × Zhonghua 6 (XZ)"...
2018: Frontiers in Plant Science
Yanghai Zhang, Yang Cui, Xuelian Zhang, Yimin Wang, Jiayang Gao, Ting Yu, Xiaoyan Lv, Chuanying Pan
Steroidogenic acute regulatory protein (StAR), primarily expressed in Leydig cells (LCs) in the mammalian testes, is essential for testosterone biosynthesis and male fertility. However, no previous reports have explored the expression profiles, alternative splicing and genetic variations of StAR gene in pig. The aim of current study was to explore the expression profiles in different tissues and different types of testicular cells (LCs; spermatogonial stem cells, SSCs; Sertoli cells, SCs), to identify different splice variants and their expression levels, as well as to detect the indel polymorphism in pig StAR gene...
May 31, 2018: Theriogenology
Oliver Schilling, Martin L Biniossek, Bettina Mayer, Brigitta Elsässer, Hans Brandstetter, Peter Goettig, Ulf-Håkan Stenman, Hannu Koistinen
In humans, three different trypsin-isoenzymes have been described. Of these, trypsin-3 appears to be functionally different from the others. In order to systematically study the specificity of the trypsin-isoenzymes, we utilized proteome-derived peptide libraries and quantitative proteomics. We found similar specificity profiles dominated by the wellcharacterized preference for cleavage after lysine and arginine. Especially trypsin-1 slightly favored lysine over arginine in this position, while trypsin-3 did not discriminate between them...
June 1, 2018: Biological Chemistry
Rattikan Suwannasing, Monchai Duangjinda, Wuttigrai Boonkum, Rutjawate Taharnklaew, Komson Tuangsithtanon
Objective: The purpose of this study was to investigate a single step genome-wide association study (ssGWAS) for identifying genomic regions affecting on reproduction trait in Landrace and Large White pigs. Methods: The traits included the number of pigs weaned per sow per year (PWSY), the number of litters per sow per year (LSY), pigs weaned per litters (PWL), born alive per litters (BAL), non-productive day (NPD) and wean to conception interval per litters (W2CL)...
May 31, 2018: Asian-Australasian Journal of Animal Sciences
Chengqi Li, Yuanyuan Wang, Nijiang Ai, Yue Li, Jiafeng Song
Genome-wide association studies (GWAS) efficiently identify genetic loci controlling traits at a relatively high resolution. In this study, variations in major early-maturation traits, including seedling period (SP), bud period (BP), flower and boll period (FBP), and growth period (GP), of 169 upland cotton accessions were investigated, and a GWAS of early maturation was performed based on a CottonSNP80K array. A total of 49,650 high-quality single-nucleotide polymorphisms (SNPs) were screened, and 29 significant SNPs located on chromosomes A6, A7, A8, D1, D2, and D9, were repeatedly identified as associated with early-maturation traits, in at least two environments or two algorithms...
June 7, 2018: Journal of Integrative Plant Biology
Yaping Liu, Aixia Xu, Fenghao Liang, Xueqin Yao, Yang Wang, Xia Liu, Yan Zhang, Jazira Dalelhan, Bingbing Zhang, Mengfan Qin, Zhen Huang, Lei Shaolin
Clubroot is an economically important disease affecting plants in the family Cruciferae worldwide. In this study, a collection of 50 Cruciferae accessions was screened using Plasmodiophora brassicae pathotype 4 in China. Eight of these demonstrated resistance, including three Chinese cabbages, two cabbages, one radish, one kale, and one Brassica juncea . The three clubroot-resistant Chinese cabbages (1003, 1007 and 1008) were then used to transfer the clubroot resistance genes to B. napus by distant hybridization combined with embryo rescue...
March 2018: Breeding Science
Fengmei Li, Jianyin Xie, Xiaoyang Zhu, Xueqiang Wang, Yan Zhao, Xiaoqian Ma, Zhanying Zhang, Muhammad A R Rashid, Zhifang Zhang, Linran Zhi, Shuyang Zhang, Jinjie Li, Zichao Li, Hongliang Zhang
Avoidance of disadvantageous genetic correlations among growth duration and yield traits is critical in developing crop varieties that efficiently use light and energy resources and produce high yields. To understand the genetic basis underlying the correlations among heading date and three major yield traits in rice, we investigated the four traits in a diverse and representative core collection of 266 cultivated rice accessions in both long-day and short-day environments, and conducted the genome-wide association study using 4...
2018: Frontiers in Plant Science
Bin Huang, Ning Zhong, Lili Xia, Guiping Yu, Hongbao Cao
Precision medicine and personalized treatment have attracted attention in recent years. However, most genetic medicines mainly target one genetic site, while complex diseases like esophageal squamous cell carcinoma (ESCC) usually present heterogeneity that involves variations of many genetic markers. Here, we seek an approach to leverage genetic data and ESCC knowledge data to forward personalized diagnosis and treatment for ESCC. First, 851 ESCC-related gene markers and their druggability were studied through a comprehensive literature analysis...
June 4, 2018: Bulletin of Mathematical Biology
Jesper Tranekjær Jørgensen, Kamilla Norregaard, Marina Simón Martín, Lene Broeng Oddershede, Andreas Kjaer
Rationale: Since its first implementation nanoparticle-assisted photothermal cancer therapy has been studied extensively, although mainly with focus on optimal nanoparticle design. However, development of efficient treatment protocols, as well as reliable and early evaluation tools in vivo , are needed to push the therapy towards clinical translation. Positron emission tomography (PET) is a non-invasive imaging technique that is currently finding extensive use for early evaluation of cancer therapies; an approach that has become of increasing interest due to its great potential for personalized medicine...
2018: Nanotheranostics
Wenxing Pang, Pengyu Fu, Xiaonan Li, Zongxiang Zhan, Sha Yu, Zhongyun Piao
The rapid spread of clubroot disease, which is caused by Plasmodiophora brassicae , threatens Brassicaceae crop production worldwide. Breeding plants that have broad-spectrum disease resistance is one of the best ways to prevent clubroot. In the present study, eight Chinese cabbage germplasms were screened using published clubroot-resistant (CR) loci-/gene-linked markers. A CR gene Crr3 potential carrier "85-74" was detected which linked to marker BRSTS61; however, "85-74" shows different responses to local pathogens "LAB-19," "LNND-2," and "LAB-10" from "CR-73" which harbors Crr3...
2018: Frontiers in Plant Science
Andriy Kochevenko, Yong Jiang, Christiane Seiler, Korana Surdonja, Sonja Kollers, Jochen Christoph Reif, Viktor Korzun, Andreas Graner
BACKGROUND: Barley (Hordeum vulgare) is an important crop cultivated across the world. Drought is a major abiotic factor compromising barley yield worldwide, therefore in modern spring barley cultivars superior seed and malting quality characteristics should be combined with reasonable level of drought tolerance. Previously we have identified a number of barley lines demonstrating the superior yield performance under drought conditions. The aim of this work was to perform a QTL analysis of malting quality traits in a doubled haploid (DH) mapping population of two elite barley lines that differ in their reaction pattern to drought stress...
June 4, 2018: BMC Plant Biology
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