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Marker assisted selection

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https://www.readbyqxmd.com/read/29054021/qprtase-modified-n-doped-carbon-quantum-dots-a-fluorescent-bioprobe-for-selective-detection-of-neurotoxin-quinolinic-acid-in-human-serum
#1
Ranjana Singh, Sunayana Kashayap, Vimal Singh, Arvind M Kayastha, Hirdyesh Mishra, Preeti Suman Saxena, Anchal Srivastava, Ranjan K Singh
Highly fluorescent nitrogen doped carbon quantum dots (NCQDs) were synthesized using microwave assisted green method. It was characterized by Transmission Electron Microscopy (TEM), FTIR, UV-Visible absorption and Photoluminiscence (PL) techniques. The NCQDs were immobilized with an enzyme named quinolinate phoshphoribosyl transferase (QPRTase). The NCQDs immobilized by QPRTase was used as a fluorescent bioprobe for the selective detection of endogenous neurotoxin quinolinic acid (QA) whose elevated level in serum is marker of many neurological disorders such as Alzheimer's, Huntington's and HIV associated dementia (HAD) as well as deficiency of vitamin B6...
October 10, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29050424/a-functional-single-nucleotide-polymorphism-in-the-tyrosinase-gene-promoter-affects-skin-color-and-transcription-activity-in-the-black-boned-chicken
#2
S Yu, J Liao, M Tang, Y Wang, X Wei, L Mao, C Zeng
The tyrosinase (TYR) gene is the major melanogenesis-related gene for skin (fur) or plumage color in mammals and birds. Genetic variation in the promoter region of a gene may affect gene expression and phenotypes. This study compared the TYR promoter region between pooled DNA (n = 8) of chickens (Gallus gallus) with black and white skin using direct sequencing. The single nucleotide polymorphism (SNP) c.-2228A>T was found to have the opposite allele distribution in the two groups. The results of genotyping in a larger population (n = 188) revealed that SNP c...
September 1, 2017: Poultry Science
https://www.readbyqxmd.com/read/29050314/applicability-of-graded-prognostic-assessment-of-lung-cancer-using-molecular-markers-to-lung-adenocarcinoma-patients-with-brain-metastases
#3
Hongwei Li, Jianhong Lian, Songyan Han, Weili Wang, Haixia Jia, Jianzhong Cao, Xiaqin Zhang, Xin Song, Sufang Jia, Jiwei Ren, Weihua Yang, Yanfeng Xi, Shengmin Lan
Several scoring systems are available to estimate prognosis and assist in selecting treatment methods for non-small cell lung cancer (NSCLC) patients with brain metastasis, including recursive partitioning analysis (RPA), basic score for brain metastases (BS-BM), and diagnosis-specific graded prognostic assessment (DS-GPA). Lung-molGPA is an update of the DS-GPA that incorporates EGFR and/or ALK mutation status. The present study tested the applicability of these four indexes in 361 lung adenocarcinoma patients with brain metastasis...
September 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29037166/taflo2-a1-an-ortholog-of-rice-flo2-is-associated-with-thousand-grain-weight-in-bread-wheat-triticum-aestivum-l
#4
Muhammad Sajjad, Xiaoling Ma, Sultan Habibullah Khan, Muhammad Shoaib, Yanhong Song, Wenlong Yang, Aimin Zhang, Dongcheng Liu
BACKGROUND: The Flo2 gene is a member of a conserved gene family in plants. This gene has been found to be related to thousand grain weight (TGW) in rice. Its orthologs in hexaploid wheat were cloned, and the haplotype variation in TaFlo2-A1 was tested for association with TGW. RESULTS: The cloned sequences of TaFlo2-A1, TaFlo2-B1 and TaFlo2-D1 contained 23, 23 and 24 exons, respectively. The deduced proteins of TaFlo2-A1 (1734 aa), TaFlo2-B1 (1698 aa) and TaFlo2-D1 (1682 aa) were highly similar (>94%) and exhibited >77% similarity with the rice FLO2 protein...
October 16, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/29037084/epr-measurements-of-reactive-oxygen-species-by-cyclic-hydroxylamine-spin-probes
#5
Sergey I Dikalov, Yuliya F Polienko, Igor A Kirilyuk
SIGNIFICANCE: Oxidative stress contributes to numerous pathophysiological conditions such as development of cancer, neurodegenerative and cardiovascular diseases. A variety of measurements of oxidative stress markers in biological systems have been developed, however, many of these methods are not specific, can produce artifacts and do not directly detect the free radicals and reactive oxygen species (ROS) that cause oxidative stress. Electron Paramagnetic Resonance (EPR) is a unique tool that allows direct measurements of free radical species...
October 17, 2017: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/29035139/novel-17-bp-deletion-in-kdm1b-gene-is-significantly-associated-with-testis-weight-in-male-piglet
#6
Yang Cui, Tao Hu, Rui Chen, Shuai Yu, Wuzi Dong, Xiaoyan Lv, Chuanying Pan
Lysine-specific demethylase 1B (KDM1B) which plays a crucial role in regulating methylation status at lysine 4 of histone 3 is important for male fertility. The aim of this study was to explore the KDM1B mRNA expression profiles and to identify novel genetic variants of the pig KDM1B gene, as well as to determine the association between these variants and testis measurement traits in male piglets. The KDM1B mRNA expression profiles indicated that this gene widely expressed in all tested organs. In addition, a novel 17-bp deletion (NC_010449...
October 16, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/29033971/genetic-dissection-of-root-morphological-traits-related-to-nitrogen-use-efficiency-in-brassica-napus-l-under-two-contrasting-nitrogen-conditions
#7
Jie Wang, Xiaoling Dun, Jiaqin Shi, Xinfa Wang, Guihua Liu, Hanzhong Wang
As the major determinant for nutrient uptake, root system architecture (RSA) has a massive impact on nitrogen use efficiency (NUE). However, little is known the molecular control of RSA as related to NUE in rapeseed. Here, a rapeseed recombinant inbred line population (BnaZNRIL) was used to investigate root morphology (RM, an important component for RSA) and NUE-related traits under high-nitrogen (HN) and low-nitrogen (LN) conditions by hydroponics. Data analysis suggested that RM-related traits, particularly root size had significantly phenotypic correlations with plant dry biomass and N uptake irrespective of N levels, but no or little correlation with N utilization efficiency (NUtE), providing the potential to identify QTLs with pleiotropy or specificity for RM- and NUE-related traits...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29032401/fine-mapping-of-the-genic-male-sterile-ms-1-gene-in-capsicum-annuum-l
#8
Kyumi Jeong, Doil Choi, Jundae Lee
The genomic region cosegregating with the genic male-sterile ms 1 gene of Capsicum annuum L. was delimited to a region of 869.9 kb on chromosome 5 through fine mapping analysis. A strong candidate gene, CA05g06780, a homolog of the Arabidopsis MALE STERILITY 1 gene that controls pollen development, was identified in this region. Genic male sterility caused by the ms 1 gene has been used for the economically efficient production of massive hybrid seeds in paprika (Capsicum annuum L.), a colored bell-type sweet pepper...
October 14, 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/29032400/fine-mapping-of-qtl-qctb10-2-that-confers-cold-tolerance-at-the-booting-stage-in-rice
#9
Jilong Li, Yinghua Pan, Haifeng Guo, Lei Zhou, Shuming Yang, Zhanying Zhang, Jiazhen Yang, Hongliang Zhang, Jinjie Li, Yawen Zeng, Zichao Li
The QTL qCTB10 - 2 controlling cold tolerance at the booting stage in rice was delimited to a 132.5 kb region containing 17 candidate genes and 4 genes were cold-inducible. Low temperature at the booting stage is a major abiotic stress-limiting rice production. Although some QTL for cold tolerance in rice have been reported, fine mapping of those QTL effective at the booting stage is few. Here, the near-isogenic line ZL31-2, selected from a BC7F2 population derived from a cross between cold-tolerant variety Kunmingxiaobaigu (KMXBG) and the cold-sensitive variety Towada, was used to map a QTL on chromosome 10 for cold tolerance at the booting stage...
October 14, 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/29025288/-state-of-the-art-molecular-diagnostics-and-therapy-of-chronic-lymphocytic-leukaemia-in-the-era-of-new-targeted-therapies
#10
Tímea Gurbity Pálfi, Viktória Fésüs, Csaba Bödör, Zita Borbényi
Chronic lymphoid leukaemia (CLL) has a heterogeneous clinical course depending on many clinical and molecular prognostic markers, which play an important role in the selection of the best treatment option. So far, TP53 disruption is the key prognostic and predictive factor assisting treatment decisions, especially in the era of novel therapies. Asymptomatic patients in early stages of the disease will still benefit from watchful waiting. In the frontline setting, chemoimmunotherapy is still the standard care in the majority of standard risk CLL patients...
October 2017: Orvosi Hetilap
https://www.readbyqxmd.com/read/29023078/polymorphism-of-insulin-like-growth-factor-1-gene-igf1-tasi-igf1-snabi-igf1-rsai-and-the-association-with-daily-gain-of-pesisir-cattle-local-breed-from-west-sumatera-indonesia
#11
Yurnalis, Arnim, Dino Eka Putra
BACKGROUND AND OBJECTIVE: The insulin-like growth factor 1 gene (IGF1) is a candidate gene for marker-assisted selection strategies. Two single nucleotide polymorphism of IGF1 gene namely IGF1/SnaBI and IGF1/TasI has been reported to be associated with production traits in several cattle breeds. One single nucleotide polymorphism in exon 4 namely IGF1/Rsa1 has a significant influence (p<0.01) on birth weight, weaning weight and average daily gain of Bali cattle. The objective of this study was to determine the IGF1/SnaBI, IGF1/TasI and IGF1/RsaI polymorphisms and their associations with average daily gain in Pesisir cattle local breed from west Sumatera Indonesia...
2017: Pakistan Journal of Biological Sciences: PJBS
https://www.readbyqxmd.com/read/29018458/genome-wide-association-study-of-major-agronomic-traits-related-to-domestication-in-peanut
#12
Xingguo Zhang, Jianhang Zhang, Xiaoyan He, Yun Wang, Xingli Ma, Dongmei Yin
Peanut (Arachis hypogaea) consists of two subspecies, hypogaea and fastigiata, and has been cultivated worldwide for hundreds of years. Here, 158 peanut accessions were selected to dissect the molecular footprint of agronomic traits related to domestication using specific-locus amplified fragment sequencing (SLAF-seq method). Then, a total of 17,338 high-quality single nucleotide polymorphisms (SNPs) in the whole peanut genome were revealed. Eleven agronomic traits in 158 peanut accessions were subsequently analyzed using genome-wide association studies (GWAS)...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29017590/review-of-the-evolution-of-insecticide-resistance-in-main-malaria-vectors-in-cameroon-from-1990-to-2017
#13
REVIEW
Christophe Antonio-Nkondjio, N Sonhafouo-Chiana, C S Ngadjeu, P Doumbe-Belisse, A Talipouo, L Djamouko-Djonkam, E Kopya, R Bamou, P Awono-Ambene, Charles S Wondji
BACKGROUND: Malaria remains a major public health threat in Cameroon and disease prevention is facing strong challenges due to the rapid expansion of insecticide resistance in vector populations. The present review presents an overview of published data on insecticide resistance in the main malaria vectors in Cameroon to assist in the elaboration of future and sustainable resistance management strategies. METHODS: A systematic search on mosquito susceptibility to insecticides and insecticide resistance in malaria vectors in Cameroon was conducted using online bibliographic databases including PubMed, Google and Google Scholar...
October 10, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29016665/qtl-mapping-for-flowering-time-and-photoperiod-insensitivity-of-cotton-gossypium-darwinii-watt
#14
Fakhriddin N Kushanov, Zabardast T Buriev, Shukhrat E Shermatov, Ozod S Turaev, Tokhir M Norov, Alan E Pepper, Sukumar Saha, Mauricio Ulloa, John Z Yu, Johnie N Jenkins, Abdusattor Abdukarimov, Ibrokhim Y Abdurakhmonov
Most wild and semi-wild species of the genus Gossypium are exhibit photoperiod-sensitive flowering. The wild germplasm cotton is a valuable source of genes for genetic improvement of modern cotton cultivars. A bi-parental cotton population segregating for photoperiodic flowering was developed by crossing a photoperiod insensitive irradiation mutant line with its pre-mutagenesis photoperiodic wild-type G. darwinii Watt genotype. Individuals from the F2 and F3 generations were grown with their parental lines and F1 hybrid progeny in the long day and short night summer condition (natural day-length) of Uzbekistan to evaluate photoperiod sensitivity, i...
2017: PloS One
https://www.readbyqxmd.com/read/29016662/fine-mapping-of-pi57-t-conferring-broad-spectrum-resistance-against-magnaporthe-oryzae-in-introgression-line-il-e1454-derived-from-oryza-longistaminata
#15
Liying Dong, Shufang Liu, Peng Xu, Wei Deng, Xundong Li, Didier Tharreau, Jing Li, Jiawu Zhou, Qun Wang, Dayun Tao, Qinzhong Yang
Wild species of the genus Oryza are excellent gene pools for improvement of agronomic traits of Asian cultivated rice. The blast resistance gene Pi57(t) in the introgression line IL-E1454 derived from Oryza longistaminata was previously mapped on rice chromosome 12. Inoculation with 322 Magnaporthe oryzae isolates collected from 6 countries indicated that Pi57(t) conferred broad spectrum resistance against M. oryzae. Two mapping populations consisting of 29070 and 10375 F2 plants derived from the crosses of resistant donor IL-E1454 with susceptible parents RD23 and Lijiangxintuanheigu respectively, were used for fine mapping of Pi57(t) locus...
2017: PloS One
https://www.readbyqxmd.com/read/28988985/facs-assisted-crispr-cas9-genome-editing-facilitates-parkinson-s-disease-modeling
#16
Jonathan Arias-Fuenzalida, Javier Jarazo, Xiaobing Qing, Jonas Walter, Gemma Gomez-Giro, Sarah Louise Nickels, Holm Zaehres, Hans Robert Schöler, Jens Christian Schwamborn
Genome editing and human induced pluripotent stem cells hold great promise for the development of isogenic disease models and the correction of disease-associated mutations for isogenic tissue therapy. CRISPR-Cas9 has emerged as a versatile and simple tool for engineering human cells for such purposes. However, the current protocols to derive genome-edited lines require the screening of a great number of clones to obtain one free of random integration or on-locus non-homologous end joining (NHEJ)-containing alleles...
September 26, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28984301/are-the-uterine-serous-carcinomas-underdiagnosed-histomorphologic-and-immunohistochemical-correlates-and-clinical-follow-up-in-high-grade-endometrial-carcinomas-initially-diagnosed-as-high-grade-endometrioid-carcinoma
#17
Shaomin Hu, Jeff L Hinson, Rahul Matnani, Michael L Cibull, Rouzan G Karabakhtsian
Histologic subclassification of high-grade endometrial carcinomas can sometimes be a diagnostic challenge when based on histomorphology alone. Here we utilized immunohistochemical markers to determine the immunophenotype in histologically ambiguous high-grade endometrial carcinomas that were initially diagnosed as pure or mixed high-grade endometrioid carcinoma, aiming to determine the utility of selected immunohistochemical panel in accurate classification of these distinct tumor types, while correlating these findings with the clinical outcome...
October 6, 2017: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
https://www.readbyqxmd.com/read/28981769/increasing-crop-yield-and-resilience-with-trehalose-6-phosphate-targeting-a-feast-famine-mechanism-in-cereals-for-better-source-sink-optimization
#18
Matthew J Paul, Maria Oszvald, Claudia Jesus, Charukesi Rajulu, Cara A Griffiths, Christine Raines
Food security is a pressing global issue. New approaches are required to break through a yield ceiling that has developed in recent years for the major crops. As important as increasing yield potential is the protection of yield from abiotic stresses in an increasingly variable and unpredictable climate. Current strategies to improve yield include conventional breeding, marker-assisted breeding, quantitative trait loci (QTLs), mutagenesis, creation of hybrids, genetic modification (GM), emerging genome-editing technologies, and chemical approaches...
July 20, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28981529/design-and-validation-of-a-90k-snp-genotyping-assay-for-the-water-buffalo-bubalus-bubalis
#19
Daniela Iamartino, Ezequiel L Nicolazzi, Curtis P Van Tassell, James M Reecy, Eric R Fritz-Waters, James E Koltes, Stefano Biffani, Tad S Sonstegard, Steven G Schroeder, Paolo Ajmone-Marsan, Riccardo Negrini, Rolando Pasquariello, Paola Ramelli, Angelo Coletta, José F Garcia, Ahmad Ali, Luigi Ramunno, Gianfranco Cosenza, Denise A A de Oliveira, Marcela G Drummond, Eduardo Bastianetto, Alessandro Davassi, Ali Pirani, Fiona Brew, John L Williams
BACKGROUND: The availability of the bovine genome sequence and SNP panels has improved various genomic analyses, from exploring genetic diversity to aiding genetic selection. However, few of the SNP on the bovine chips are polymorphic in buffalo, therefore a panel of single nucleotide DNA markers exclusive for buffalo was necessary for molecular genetic analyses and to develop genomic selection approaches for water buffalo. The creation of a 90K SNP panel for river buffalo and testing in a genome wide association study for milk production is described here...
2017: PloS One
https://www.readbyqxmd.com/read/28979832/mapping-and-validation-of-qtls-for-cold-tolerance-at-seedling-stage-in-rice-from-an-indica-cultivar-habiganj-boro-vi-hbj-bvi
#20
Partha S Biswas, Hasina Khatun, Nomita Das, Md Mahathir Sarker, M Anisuzzaman
Yellowing, stunting, and seedling death associated with cold stress is a common problem in many Asian countries for winter rice cultivation. Improvement of cultivars through marker-assisted selection of QTLs for cold tolerance at seedling stage from locally adapted germplasm/cultivar is the most effective and sustainable strategy to resolve this problem. A study was undertaken to map QTLs from 151 F2:3 progenies of a cross between a cold susceptible variety, BR1 and a locally adapted traditional indica cultivar, Hbj...
December 2017: 3 Biotech
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