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https://www.readbyqxmd.com/read/29036988/-characteristics-of-molecular-typing-and-drug-resistance-of-67-salmonella-paratyphi-a-isolated-in-zhengzhou-from-2013-to-2015
#1
J Y Zhao, S Y Zhang, B F Zhang, Y J Mu, J Su, X Y Huang, B L Xu
Objective: To investigate the antimicrobial resistance and pulsed field gel electrophoresis (PFGE) patterns of S.paratyphi A strains in Zhengzhou city isolated from sentinel hospitals in 2013-2015. Methods: According to Salmonella molecular typing and K-B drug susceptibility testing method published by international PulseNet bacterial infectious disease monitoring network and USA Clinical and Laboratory Standards Institute (CLSI2015), we analyzed drug sensitivity and PFGE molecular characteristics of 67 S.paratyphi A strains(11 strains in 2013, 7 strains in 2014, 49 strains in 2015) isolated from blood and stool samples in two sentinel hospitals of fever with rash syndrome surveillance system established in Zhengzhou city in 2013-2015...
October 6, 2017: Zhonghua Yu Fang Yi Xue za Zhi [Chinese Journal of Preventive Medicine]
https://www.readbyqxmd.com/read/29036576/currbs-simple-and-robust-evaluation-of-enzyme-combinations-for-reduced-representation-approaches
#2
Daniel E Martin-Herranz, António J M Ribeiro, Felix Krueger, Janet M Thornton, Wolf Reik, Thomas M Stubbs
DNA methylation is an important epigenetic modification in many species that is critical for development, and implicated in ageing and many complex diseases, such as cancer. Many cost-effective genome-wide analyses of DNA modifications rely on restriction enzymes capable of digesting genomic DNA at defined sequence motifs. There are hundreds of restriction enzyme families but few are used to date, because no tool is available for the systematic evaluation of restriction enzyme combinations that can enrich for certain sites of interest in a genome...
September 19, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29036575/peptides-of-the-variable-igg-domain-as-potential-biomarker-candidates-in-primary-open-angle-glaucoma-poag
#3
Carsten Schmelter, Natarajan Perumal, Sebastian Funke, Katharina Bell, Norbert Pfeiffer, Franz H Grus
Autoantibody profiling has gained increasing interest in the research field of glaucoma promising the detection of highly specific and sensitive marker candidates for future diagnostic purposes. Recent studies demonstrated that immune responses are characterized by the expression of congruent or similar complementarity determining regions (CDR) in different individuals and could be used as molecular targets in biomarker discovery. Main objective of this study was to characterize glaucoma-specific peptides from the variable region of sera-derived immunoglobulins using liquid chromatography - mass spectrometry (LC-MS) - based quantitative proteomics...
September 22, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/29036322/tgbs%C3%A2-genotyping-by-sequencing-enables-reliable-genotyping-of-heterozygous-loci
#4
Alina Ott, Sanzhen Liu, James C Schnable, Cheng-Ting 'Eddy' Yeh, Kai-Sin Wang, Patrick S Schnable
Conventional genotyping-by-sequencing (cGBS) strategies suffer from high rates of missing data and genotyping errors, particularly at heterozygous sites. tGBS® genotyping-by-sequencing is a novel method of genome reduction that employs two restriction enzymes to generate overhangs in opposite orientations to which (single-strand) oligos rather than (double-stranded) adaptors are ligated. This strategy ensures that only double-digested fragments are amplified and sequenced. The use of oligos avoids the necessity of preparing adaptors and the problems associated with inter-adaptor annealing/ligation...
September 25, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29036014/a-family-with-two-cases-of-melanocytic-tumors-and-fragile-x-syndrome
#5
Candice Lesage, Isabelle Coupier, Bernard Guillot
Fragile X syndrome (FXS), a leading cause of inherited intellectual disability, most commonly results from an expansion of the CGG trinucleotide repeat in the fragile X mental retardation 1 (FMR1) gene to more than 200 copies (full mutation). The FXS phenotype differs by sex and is associated with intellectual and cognitive impairment, characteristic physical features, epilepsy, and/or behavioral challenges including autism spectrum disorder. In this patient population, tumors involving blood cells, digestive organs, the central nervous system, and testes have been described, but melanocytic tumors have not been reported...
October 13, 2017: Melanoma Research
https://www.readbyqxmd.com/read/29035973/the-relevance-of-dietary-protein-after-bariatric-surgery-what-do-we-know
#6
Nele Steenackers, Ina Gesquiere, Christophe Matthys
PURPOSE OF REVIEW: The growing obesity epidemic is associated with an increased demand for bariatric surgery with Roux-en-Y Gastric Bypass and Sleeve Gastrectomy as the most widely performed procedures. Despite beneficial consequences, nutritional complications may arise because of anatomical and physiological changes of the gastrointestinal tract. The purpose of this review is to provide an update of the recent additions to our understanding of the impact of bariatric surgery on the intake, digestion and absorption of dietary protein...
October 14, 2017: Current Opinion in Clinical Nutrition and Metabolic Care
https://www.readbyqxmd.com/read/29035861/the-molecular-identification-of-calodium-hepaticum-in-the-wild-brown-rat-rattus-norvegicus-in-poland
#7
Katarzyna Buńkowska-Gawlik, Agnieszka Perec-Matysiak, Klaudia Burzyńska, Joanna Hildebrand
Calodium hepaticum is a parasitic nematode found primarily in rodents but known to infect numerous other mammal species, and causing in humans the hepatic calodiasis. Herein, we present the first finding of C. hepaticum in Rattus norvegicus in Poland. In this study, we have used the combined pepsin-HCl digestion and molecular techniques to detect C. hepaticum infection in the liver. The results confirm the usefulness of molecular approaches for investigating the biology and epidemiology of C. hepaticum. Additionally in this study, the parts of the small subunit rRNA (18S rRNA) gene of Aonchotheca annulosa from bank vole, Aonchotheca erinacei and Eucoleus sp...
December 20, 2017: Acta Parasitologica
https://www.readbyqxmd.com/read/29035853/the-gastrointestinal-nematodes-of-paramelomys-lorentzii-and-mammelomys-spp-rodentia-muridae-with-descriptions-of-a-new-genus-and-three-new-species-heligmonellidae-from-papua-new-guinea-and-indonesia
#8
Lesley R Smales
Unidentified cestodes and 14 species of nematode and larvae that could not be placed to family level, were collected from the digestive tracts of 27 individuals of Paramelomys lorentzii, four of Mammelomys lanosus and a single M. rattoides from Papua New Guinea and Papua Indonesia. Of these three were new species. Hughjonestrongylus woolleyae sp. nov. can be distinguished from its congeners in having up to 26 ridges in the synlophe and a dissymmetric dorsal ray. Parvinema bafunminensis gen. nov., sp. nov. can be distinguished from all other genera in the family by the combination of features in the synlophe; with a carene, up to 17 ridges, and the pattern of ridge sizes...
December 20, 2017: Acta Parasitologica
https://www.readbyqxmd.com/read/29035679/performance-and-membrane-fouling-of-a-step-fed-submerged-membrane-sequencing-batch-reactor-treating-swine-biogas-digestion-slurry
#9
Zhiying Han, Shixia Chen, Xiaochang Lin, Hongjun Yu, Li'an Duan, Zhangying Ye, Yanbo Jia, Songming Zhu, Dezhao Liu
To identify the performance of step-fed submerged membrane sequencing batch reactor (SMSBR) treating swine biogas digestion slurry and to explore the correlation between microbial metabolites and membrane fouling within this novel reactor, a lab-scale step-fed SMSBR was operated under nitrogen loading rate of 0.026, 0.052 and 0.062 g NH4(+)-N (gVSS·d)(-1). Results show that the total removal efficiencies for NH4(+)-N, total nitrogen and chemical oxygen demand in the reactor (>94%, >89% and >97%, respectively) were high during the whole experiment...
October 16, 2017: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/29035602/effect-of-pre-exercise-ingestion-of-modified-amylomaize-starch-on-glycemic-response-while-cycling
#10
Rachel B Parks, Hector F Angus, Douglas S King, Rick Sharp
Amylomaize-7 is classified as a resistant corn starch and is 68% digestible. When modified by partial hydrolysis in ethanol and hydrochloric acid, its digestibility is 92%, yet retains its low glycemic and insulinemic properties. The purpose of this study was to characterize the metabolic response when modified amylomaize-7 or dextrose is consumed in the hour before exercise, and to compare the effect on performance of a brief high-intensity cycling trial. Ten male trained cyclists were given 1 g/kg body mass of dextrose (DEX) or modified amylomaize-7 (AMY-7) or a flavored water placebo (PL) 45 min prior to exercise on a cycle ergometer...
October 16, 2017: International Journal of Sport Nutrition and Exercise Metabolism
https://www.readbyqxmd.com/read/29035569/digest
#11
(no author information available yet)
No abstract text is available yet for this article.
October 16, 2017: Adapted Physical Activity Quarterly: APAQ
https://www.readbyqxmd.com/read/29035303/optimization-of-bicomponent-electrospun-fibers-for-therapeutic-use-post-treatments-to-improve-chemical-and-biological-stability
#12
Antonio Papa, Vincenzo Guarino, Valentina Cirillo, Olimpia Oliviero, Luigi Ambrosio
Bicomponent electrospun nanofibers based on the combination of synthetic (i.e., aliphatic polyesters such as polycaprolactone (PCL)) and natural proteins (i.e., gelatin) have been extensively investigated as temporary platforms to instruct cells by the release of molecular/pharmaceutical signals for the regeneration of several tissues. Here, water soluble proteins (i.e., gelatin), strictly embedded to PCL, act as carriers of bioactive molecules, thus improving bioavailability and supporting cell activities during in vitro regeneration...
October 16, 2017: Journal of Functional Biomaterials
https://www.readbyqxmd.com/read/29035051/integrating-carbamylation-and-ultraviolet-photodissociation-mass-spectrometry-for-middle-down-proteomics
#13
James D Sanders, Sylvester M Greer, Jennifer S Brodbelt
The most popular bottom-up proteomics workflow uses trypsin to enzymatically cleave proteins C-terminal to lysine and arginine residues prior to LCMS/MS analysis of the resulting peptides. The high frequency of these residues generates short peptides, some of which are too small or uninformative for optimal analysis and which potentially contribute to gaps in sequence coverage of proteins. Analysis of larger peptides, termed "middle-down", has the potential to span greater sections of protein sequences if the larger peptides are adequately characterized based on their fragmentation patterns...
October 16, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29034989/neurological-presentations-revealing-acquired-copper-deficiency-diagnosis-features-etiologies-and-evolution-in-seven-patients
#14
Aurélia Poujois, Nouzha Djebrani-Oussedik, Fabienne Ory-Magne, France Woimant
BACKGROUND: acquired copper deficiency (ACD) is a rare condition usually diagnosed from haematological changes. AIMS: characterize the diagnosis features and the evolution of patients with ACD revealed by neurological symptoms. METHODS: clinical, biological and MRI data were prospectively analysed at diagnosis and during follow-up under copper supplementation. RESULTS: seven patients were studied over a 5-year period...
October 16, 2017: Internal Medicine Journal
https://www.readbyqxmd.com/read/29034638/-construction-of-spider-silk-functional-platform-via-intein-trans-splicing
#15
Senzhu Lin, Gefei Chen, Qing Meng
To provide technical support for spider silk functional modification, we developed a simple and efficient functional platform via intein trans-splicing. Small ubiquitin-related modifier protein (SUMO) was fused to the recombinant spider silk protein (W2CT) by peptide bond via S0 split intein Ssp DnaB trans-splicing, resulting in a protein SUMOW2CT. However, incorporation of exogenous protein led to mechanical property defect and lower fiber yield, and also slowed down the fiber assembly velocity but no obvious differences in supercontraction and chemical resistance when compared with fibers from W2CT (W)...
December 25, 2016: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/29034634/-construction-and-characterization-of-type-iii-secretion-system-of-attenuated-salmonella-typhimurium
#16
Chuan Yu, Chongkai Zhai, Chengshui Liao, Zuhua Yu, Lei He, Yanyan Jia, Jing Li, Chunjie Zhang, Xiangchao Cheng
In order to develop a recombinant attenuated Salmonella typhimurium as oral live vaccine vector, we constructed recombinant plasmid pYA-sopENt100 by replacing the trc promoter with the sopE promoter and secretion signal sequence sopENt100 of Salmonella typhimurium on the basis of plasmid pYA3493. Then, the complementary plasmid pYA-sopENt100 was transformed into ΔcrpΔasdSL1344 by electroporation to generate attenuated Salmonella typhimurium type III secretion system ΔcrpΔasdSL1344 (pYA-sopENt100). We further characterized ΔcrpΔasdSL1344 (pYA-sopENt100)...
December 25, 2016: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/29034337/new-biomarkers-of-post-settlement-growth-in-the-sea-urchin-strongylocentrotus-purpuratus
#17
Alyaa Elsaid Abdelaziz Fadl, Magdy Elsayed Mahfouz, Mona Mabrouk Taha El-Gamal, Andreas Heyland
Some sea urchins, including the purple sea urchin Strongylocentrotus purpuratus, have been successfully used in aquaculture, but their slow growth and late reproduction are challenging to overcome when developing efficient aquaculture production techniques. S. purpuratus develops via an indirect life history that is characterized by a drastic settlement process at the end of a larval period that lasts for several weeks. During this transition, the bilateral larva is transformed into a pentaradial juvenile, which will start feeding and growing in the benthic habitat...
October 2017: Heliyon
https://www.readbyqxmd.com/read/29034180/significance-of-fermentation-process-on-biochemical-properties-of-phaseolus-vulgaris-red-beans
#18
Anteneh Worku, Omprakash Sahu
Phaseolus vulgaris L. beans have been characterized as a nearly perfect food in all around the world. It is consider functional in terms of providing nutrients and energy to sustain daily life. Red bean contain high protein, mineral, fibres and chemically diverse micronutrient composition, which can be affected by processing. The main aim of this work is to investigate the effect of open and controlled fermentation on the proximate composition, mineral elements, antinutritional factors and flatulence- causing oligosaccharides of a domesticated bean (Anger, conscope and Loko)...
December 2017: Biotechnology Reports
https://www.readbyqxmd.com/read/29034005/in-vitro-and-in-vivo-modeling-of-lipid-bioaccessibility-and-digestion-from-almond-muffins-the-importance-of-the-cell-wall-barrier-mechanism
#19
Terri Grassby, Giuseppina Mandalari, Myriam M-L Grundy, Cathrina H Edwards, Carlo Bisignano, Domenico Trombetta, Antonella Smeriglio, Simona Chessa, Shuvra Ray, Jeremy Sanderson, Sarah E Berry, Peter R Ellis, Keith W Waldron
This study compares in vitro and in vivo models of lipid digestion from almond particles within a complex food matrix (muffins) investigating whether the cell-wall barrier regulates the bioaccessibility of nutrients within this matrix. Muffins containing small (AF) or large (AP) particles of almond were digested in triplicate using an in vitro dynamic gastric model (DGM, 1 h) followed by a static duodenal digestion (8 h). AF muffins had 97.1 ± 1.7% of their lipid digested, whereas AP muffins had 57.6 ± 1...
October 2017: Journal of Functional Foods
https://www.readbyqxmd.com/read/29033945/improved-efficacy-of-oral-immunotherapy-using-non-digestible-oligosaccharides-in-a-murine-cow-s-milk-allergy-model-a-potential-role-for-foxp3-regulatory-t-cells
#20
Marlotte M Vonk, Mara A P Diks, Laura Wagenaar, Joost J Smit, Raymond H H Pieters, Johan Garssen, Betty C A M van Esch, Léon M J Knippels
BACKGROUND: Oral immunotherapy (OIT) is a promising therapeutic approach to treat food allergic patients. However, there are some concerns regarding its safety and long-term efficacy. The use of non-digestible oligosaccharides might improve OIT efficacy since they are known to directly modulate intestinal epithelial and immune cells in addition to acting as prebiotics. AIM: To investigate whether a diet supplemented with plant-derived fructo-oligosaccharides (FOS) supports the efficacy of OIT in a murine cow's milk allergy model and to elucidate the potential mechanisms involved...
2017: Frontiers in Immunology
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