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https://www.readbyqxmd.com/read/27902304/pseudomonas-canadensis-sp-nov-a-biological-control-agent-isolated-from-a-field-plot-under-long-term-mineral-fertilization
#1
James T Tambong, Renin Xu, Eden Bromfield
The bacterial strain 2-92T isolated from a field plot under long-term (>40 years) mineral fertilization exhibited in vitro antagonistic properties against fungal pathogens. A polyphasic approach was undertaken to verify its taxonomic status. Strain 2-92T is gram-negative, aerobic, non-spore-forming, motile by one or more flagella, and oxidase-, catalase- and urease-positive. Optimal growth temperature of strain 2-92T is 30 °C. 16S rRNA gene sequence analysis demonstrated that the strain is related to species of the genus Pseudomonas...
November 28, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27894820/quantitative-detection-of-periodontopathic-bacteria-in-lower-respiratory-tract-specimens-by-real-time-pcr
#2
Kentaro Nagaoka, Katsunori Yanagihara, Yosuke Harada, Koichi Yamada, Yohei Migiyama, Yoshitomo Morinaga, Koichi Izumikawa, Shigeru Kohno
The presence of common periodontopathic bacteria, the Fusobacterium nucleatum-periodonticum-simiae group, Prevotella intermedia, and Porphyromonas gingivalis was determined from respiratory tract specimens of bacterial pneumonia by real-time PCR using universal and species-specific TaqMan probe/primer sets. 42 patients with infectious pneumonia and 45 patients without infectious pneumonia were retrospectively enrolled in clinical studies. Periodontopathic bacterial DNA was found in 57.1% cases of infectious pneumonia and 31...
November 26, 2016: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/27848154/jasmonic-acid-and-ethylene-signaling-pathways-regulate-glucosinolate-levels-in-plants-during-rhizobacteria-induced-systemic-resistance-against-a-leaf-chewing-herbivore
#3
Nurmi Pangesti, Michael Reichelt, Judith E van de Mortel, Eleni Kapsomenou, Jonathan Gershenzon, Joop J A van Loon, Marcel Dicke, Ana Pineda
Beneficial soil microbes can promote plant growth and induce systemic resistance (ISR) in aboveground tissues against pathogens and herbivorous insects. Despite the increasing interest in microbial-ISR against herbivores, the underlying molecular and chemical mechanisms of this phenomenon remain elusive. Using Arabidopsis thaliana and the rhizobacterium Pseudomonas simiae WCS417r (formerly known as P. fluorescens WCS417r), we here evaluate the role of the JA-regulated MYC2-branch and the JA/ET-regulated ORA59-branch in modulating rhizobacteria-ISR to Mamestra brassicae by combining gene transcriptional, phytochemical, and herbivore performance assays...
November 15, 2016: Journal of Chemical Ecology
https://www.readbyqxmd.com/read/27792159/pseudomonas-aeruginosa-type-iii-secretory-toxin-exou-and-its-predicted-homologs
#4
REVIEW
Teiji Sawa, Saeko Hamaoka, Mao Kinoshita, Atsushi Kainuma, Yoshifumi Naito, Koichi Akiyama, Hideya Kato
Pseudomonas aeruginosa ExoU, a type III secretory toxin and major virulence factor with patatin-like phospholipase activity, is responsible for acute lung injury and sepsis in immunocompromised patients. Through use of a recently updated bacterial genome database, protein sequences predicted to be homologous to Ps. aeruginosa ExoU were identified in 17 other Pseudomonas species (Ps. fluorescens, Ps. lundensis, Ps. weihenstephanensis, Ps. marginalis, Ps. rhodesiae, Ps. synxantha, Ps. libanensis, Ps. extremaustralis, Ps...
October 26, 2016: Toxins
https://www.readbyqxmd.com/read/27698571/seed-micromorphology-of-orchis-tourn-ex-l-orchidaceae-and-allied-genera-growing-in-edirne-province-turkey
#5
Necmettin Güler
In this study, the seed micromorphologies of eight taxa of Anacamptis, Neotinea and Orchis growing around Edirne province (Turkey) were investigated using light microscopy and scanning electron microscopy (SEM). Slides prepared with glycerin jelly were used for measurements under the light microscope and fine details of seed testae characteristics were observed with SEM. Seeds of the investigated orchid taxa are fusiform shaped and of different shades of brown. Their lengths and widths are different among the taxa and range between 0...
2016: PhytoKeys
https://www.readbyqxmd.com/read/27616445/species-diversity-and-molecular-characterization-of-nontuberculous-mycobacteria-in-hospital-water-system-of-a-developing-country-iran
#6
Davood Azadi, Hasan Shojaei, Mahnaz Pourchangiz, Ramin Dibaj, Masoumeh Davarpanah, Abass Daei Naser
BACKGROUND: Hospital environment is of crucial importance in cross-transmission of opportunistic pathogens to the patients. Nontuberculous mycobacteria have the remarkable capability to withstand the adverse condition of hospital environments and pose a potential threat to the health of patients. The current study aimed to assess the frequency and diversity of mycobacteria in hospital water of a developing country using a combination of conventional and molecular methods. METHODS: A total of 148 hospital water samples collected from 38 hospitals were analyzed for the presence of mycobacteria using standard protocols for isolation and characterization of the isolates...
September 9, 2016: Microbial Pathogenesis
https://www.readbyqxmd.com/read/27613234/description-of-peptococcus-simiae-sp-nov-isolated-from-rhesus-macaque-feces-and-emended-description-of-genus-peptococcus
#7
Andrei N Shkoporov, Boris A Efimov, Ivanela Kondova, Boudewijn Ouwerling, Andrei V Chaplin, Victoria A Shcherbakova, Jan A M Langermans
A study of the fecal microbiome in three healthy female rhesus macaques revealed the presence of a novel obligately anaerobic, chemoorganoheterotrophic, non-sporing, coccoid, non-motile, Gram-stain-positive bacterial species. Three strains of this species, designated as M108T, M916-1/1, and M919-2/1, were non-hemolytic, H2S-positive, catalase positive, bile- and NaCl-sensitive and required peptone for growth. Strains also were asaccharolytic, able to utilize sulfite, thiosulfate and elemental sulfur as electron acceptors, and produced acetic and butyric acids as metabolic end products...
September 9, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27491995/insights-into-the-genome-sequences-of-an-n-acyl-homoserine-lactone-molecule-producing-two-pseudomonas-spp-isolated-from-the-arctic
#8
Akhilandeswarre Dharmaprakash, Dinesh Reghunathan, Krishnakutty C Sivakumar, Manoj Prasannakumar, Sabu Thomas
We report for the first time the draft genome sequence of two psychrotrophic Pseudomonas species, Pseudomonas simiae RGCB 73 and Pseudomonas brenneri RGCB 108, from the Arctic that produce more than one acyl homoserine lactone molecule of varied N-acyl length. The study confirms the presence of a LuxR-LuxI (type) mediated quorum-sensing system in both the Pseudomonas species and enables us to understand the role of quorum sensing in their survival in extremely cold environments.
2016: Genome Announcements
https://www.readbyqxmd.com/read/27439917/pgpr-mediated-expression-of-salt-tolerance-gene-in-soybean-through-volatiles-under-sodium-nitroprusside
#9
Anukool Vaishnav, Sarita Kumari, Shekhar Jain, Ajit Varma, Narendra Tuteja, Devendra Kumar Choudhary
Increasing evidence shows that nitric oxide (NO), a typical signaling molecule plays important role in development of plant and in bacteria-plant interaction. In the present study, we tested the effect of sodium nitroprusside (SNP)-a nitric oxide donor, on bacterial metabolism and its role in establishment of PGPR-plant interaction under salinity condition. In the present study, we adopted methods namely, biofilm formation assay, GC-MS analysis of bacterial volatiles, chemotaxis assay of root exudates (REs), measurement of electrolyte leakage and lipid peroxidation, and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for gene expression...
November 2016: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/27423733/comparative-evaluation-of-the-nested-its-pcr-against-the-18s-pcr-rflp-in-a-survey-of-bovine-trypanosomiasis-in-kwale-county-kenya
#10
Steven Odongo, Vincent Delespaux, Maina Ngotho, Serkalem Mindaye Bekkele, Stefan Magez
We compared the nested internal transcribed spacer (ITS) PCR and the 18S PCR-RFLP (restriction-fragment length polymorphism) pan-trypanosome assays in a cross-sectional survey of bovine trypanosomiasis in 358 cattle in Kwale County, Kenya. The prevalence of trypanosomiasis as determined by the nested ITS PCR was 19.6% (70/358) and by 18S PCR-RFLP was 16.8% (60/358). Of the pathogenic trypanosomes detected, the prevalence of Trypanosoma congolense and Trypanosoma vivax was greater than that of Trypanosoma simiae The nested ITS PCR detected 83 parasite events, whereas the 18S PCR-RFLP detected 64; however, overall frequencies of infections and the parasite events detected did not differ between the assays (χ(2) = 0...
September 2016: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/27262751/nontuberculous-mycobacteria-in-cystic-fibrosis-patients-on-the-island-of-gran-canaria-a-population-study
#11
M Isolina Campos-Herrero, Francisco Javier Chamizo, José Antonio Caminero, Raúl Gilarranz, Gonzalo Cabrera, José Cuyás
OBJECTIVE: To determine the prevalence of nontuberculous mycobacteria (NTM) colonization and disease in cystic fibrosis (CF) patients. PATIENTS AND METHODS: All the CF patients followed-up from 2002 to 2012 with three acid-fast bacilli (AFB) cultures were included. The American Thoracic Society (ATS) criteria for NTM lung disease were applied. RESULTS: Forty-four of the 53 patients being followed-up were included. The mean time of follow-up was 7...
August 2016: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/27216812/molecular-identification-of-different-trypanosome-species-and-subspecies-in-tsetse-flies-of-northern-nigeria
#12
Clement Isaac, Marc Ciosi, Alana Hamilton, Kathleen Maria Scullion, Peter Dede, Igho Benjamin Igbinosa, Oyebiguwa Patrick Goddey Nmorsi, Dan Masiga, C Michael R Turner
BACKGROUND: Animal African Trypanosomiasis (AAT) is caused by several species of trypanosomes including Trypanosoma congolense, T. vivax, T. godfreyi, T. simiae and T. brucei. Two of the subspecies of T. brucei also cause Human African Trypanosomiasis. Although some of them can be mechanically transmitted by biting flies; these trypanosomes are all transmitted by tsetse flies which are the cyclical vectors of Trypanosoma congolense, T. godfreyi, T. simiae and T. brucei. We present here the first report assessing the prevalence of trypanosomes in tsetse flies in Nigeria using molecular tools...
2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27169158/tuberculosis-burden-in-lebanon-evolution-and-current-status
#13
George F Araj, Antoine Saade, Lina Y Itani, Aline Z Avedissian
BACKGROUND: In the absence of surveillance data and consolidated information about tuberculosis (TB) and its drug resistance burden in Lebanon, this retrospective study was conducted to shed light on TB and its other relevant aspects over the last 15 years in this country. METHODS: To generate the TB data for this retrospective study, two main sources were used: 1) the records of patients in the National Tuberculosis Program (NTP); 2) the results of the Clinical Microbiology Laboratory (CML) at the American University of Beirut Medical Center (AUBMC)...
January 2016: Le Journal Médical Libanais. the Lebanese Medical Journal
https://www.readbyqxmd.com/read/27154468/are-simakobu-simias-concolor-loud-calls-energetically-costly-signals
#14
Wendy M Erb, Thomas Ziegler, Nurul S Lestari, Kurt Hammerschmidt
OBJECTIVES: Male vocal displays play an important role in sexual selection in many species. If there are costs or constraints, calls may convey honest information about the caller. We studied the loud calls of male simakobu (Simias concolor), a sexually dimorphic primate that resides in one-male groups, on Siberut Island, Indonesia. MATERIALS AND METHODS: We combined behavioral and ecological data with acoustic analysis to determine the influence of energy costs and status on calling effort...
September 2016: American Journal of Physical Anthropology
https://www.readbyqxmd.com/read/27023813/diversity-of-endophytic-pseudomonas-in-halimione-portulacoides-from-metal-loid-polluted-salt-marshes
#15
Jaqueline Rocha, Marta Tacão, Cátia Fidalgo, Artur Alves, Isabel Henriques
Phytoremediation assisted by bacteria is seen as a promising alternative to reduce metal contamination in the environment. The main goal of this study was to characterize endophytic Pseudomonas isolated from Halimione portulacoides, a metal-accumulator plant, in salt marshes contaminated with metal(loid)s. Phylogenetic analysis based on 16S rRNA and gyrB genes showed that isolates affiliated with P. sabulinigri (n = 16), P. koreensis (n = 10), P. simiae (n = 5), P. seleniipraecipitans (n = 2), P...
July 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/26919540/new-pseudomonas-spp-are-pathogenic-to-citrus
#16
Farid Beiki, Antonio Busquets, Margarita Gomila, Heshmat Rahimian, Jorge Lalucat, Elena García-Valdés
Five putative novel Pseudomonas species shown to be pathogenic to citrus have been characterized in a screening of 126 Pseudomonas strains isolated from diseased citrus leaves and stems in northern Iran. The 126 strains were studied using a polyphasic approach that included phenotypic characterizations and phylogenetic multilocus sequence analysis. The pathogenicity of these strains against 3 cultivars of citrus is demonstrated in greenhouse and field studies. The strains were initially grouped phenotypically and by their partial rpoD gene sequences into 11 coherent groups in the Pseudomonas fluorescens phylogenetic lineage...
2016: PloS One
https://www.readbyqxmd.com/read/26887928/increased-isolation-of-nontuberculous-mycobacteria-among-tb-suspects-in-northeastern-tanzania-public-health-and-diagnostic-implications-for-control-programmes
#17
Abubakar S Hoza, Sayoki G M Mfinanga, Arne C Rodloff, Irmgard Moser, Brigitte König
BACKGROUND: Non-tuberculous mycobacteria (NTM) are increasingly reported worldwide associated with human disease. Defining the significance of NTM in settings with endemic tuberculosis (TB) requires the discrimination of NTM from TB in suspect patients. Correct and timely identification of NTM will impact both therapy and epidemiology of TB and TB-like diseases. The present study aimed at determining the frequency and diversity of NTM among TB suspects in northeastern Tanzania. METHODS: A cross-sectional study was conducted between November 2012 through January 2013...
2016: BMC Research Notes
https://www.readbyqxmd.com/read/26830772/natural-genetic-variation-in-arabidopsis-for-responsiveness-to-plant-growth-promoting-rhizobacteria
#18
Paul C A Wintermans, Peter A H M Bakker, Corné M J Pieterse
The plant growth-promoting rhizobacterium (PGPR) Pseudomonas simiae WCS417r stimulates lateral root formation and increases shoot growth in Arabidopsis thaliana (Arabidopsis). These plant growth-stimulating effects are partly caused by volatile organic compounds (VOCs) produced by the bacterium. Here, we performed a genome-wide association (GWA) study on natural genetic variation in Arabidopsis for the ability to profit from rhizobacteria-mediated plant growth-promotion. To this end, 302 Arabidopsis accessions were tested for root architecture characteristics and shoot fresh weight in response to exposure to WCS417r...
April 2016: Plant Molecular Biology
https://www.readbyqxmd.com/read/26712619/induced-drought-tolerance-through-wild-and-mutant-bacterial-strain-pseudomonas-simiae-in-mung-bean-vigna-radiata-l
#19
Sarita Kumari, Anukool Vaishnav, Shekhar Jain, Ajit Varma, Devendra Kumar Choudhary
The present study focused on the overproducing mutant of a plant growth promoting rhizobacterium (PGPR) Pseudomonas simiae strain AU (MTCC-12057) for significant drought tolerance in mung bean plants. Five mutants namely AU-M1, AU-M2, AU-M3, AU-M4 and AU-M5 were made after treatment of wild type strain with N-methyl-N-nitro-N-nitrosoguanidine. Mutant strain AU-M4 was recorded for enhanced ACC deaminase (ACC-D) activity, indole acetic acid (IAA) production and inorganic phosphate (Pi) solubilization compared to wild strain and other four mutant strains under drought condition...
January 2016: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/26508940/nontuberculous-mycobacterial-lung-disease-caused-by-mycobacterium-simiae-the-first-reported-case-in-south-korea
#20
Suk Hyeon Jeong, Su-Young Kim, Hyun Lee, Jun Soo Ham, Keum Bit Hwang, Subin Hwang, Sun Hye Shin, Myung Jin Chung, Seung Heon Lee, Sung Jae Shin, Won-Jung Koh
This is a report of the first South Korean case of a lung disease caused by Mycobacterium simiae. The patient was a previously healthy 52-year-old female. All serial isolates were identified as M. simiae by multi-locus sequencing analysis, based on hsp65, rpoB, 16S-23S rRNA internal transcribed spacer, and 16S rRNA fragments. A chest radiography revealed deterioration, and the follow-up sputum cultures were persistently positive, despite combination antibiotic treatment, including azithromycin, ethambutol, and rifampin...
October 2015: Tuberculosis and Respiratory Diseases
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