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https://www.readbyqxmd.com/read/29188063/high-rates-of-nontuberculous-mycobacteria-isolation-from-patients-with-presumptive-tuberculosis-in-iran
#1
M J Nasiri, H Dabiri, A A I Fooladi, S Amini, G Hamzehloo, M M Feizabadi
Nontuberculous mycobacteria (NTM) can cause disease which can be indistinguishable from tuberculosis (TB), posing a diagnostic and therapeutic challenge, particularly in low- and middle-income settings. We aimed to investigate the mycobacterial agents associated with presumptive clinical pulmonary TB in Iran. A total of 410 mycobacterial isolates, obtained between March 2014 and January 2016, from 7600 clinical samples taken from consecutive cases of presumptive diagnosis of TB were identified. Phenotypic and molecular tests were used to identify the isolated organisms to the species level...
January 2018: New Microbes and New Infections
https://www.readbyqxmd.com/read/29109708/differential-identification-of-mycobacterial-species-using-high-resolution-melting-analysis
#2
Azar D Khosravi, Mohammad Hashemzadeh, Abdolrazagh Hashemi Shahraki, Ali Teimoori
Infections caused by non-tuberculous mycobacteria (NTM) is increasing wordwide. Due to the difference in treatment of NTM infections and tuberculosis, rapid species identification of mycobacterial clinical isolates is necessary for the effective management of mycobacterial diseases treatment and their control strategy. In this study, a cost-effective technique, real-time PCR coupled with high-resolution melting (HRM) analysis, was developed for the differentiation of Mycobacterial species using a novel rpoBC sequence...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29061160/molecular-prevalence-of-trypanosome-infections-in-cattle-and-tsetse-flies-in-the-maasai-steppe-northern-tanzania
#3
Mary Simwango, Anibariki Ngonyoka, Happiness J Nnko, Linda P Salekwa, Moses Ole-Neselle, Sharadhuli I Kimera, Paul S Gwakisa
BACKGROUND: African trypanosomosis is a disease of public health and economic importance that poses a major threat to the livelihoods of people living in the Maasai Steppe, where there is a significant interaction between people, livestock and wildlife. The vulnerability of the Maasai people to the disease is enhanced by the interaction of their cattle, which act as vehicles for trypanosomes, and tsetse flies close to wildlife in protected areas. This study was aimed at identification of trypanosome infections circulating in cattle and tsetse flies in order to understand their distribution and prevalence in livestock/wildlife interface areas in the Maasai Steppe...
October 23, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29030295/the-new-phylogeny-of-the-genus-mycobacterium-the-old-and-the-news
#4
Enrico Tortoli, Tarcisio Fedrizzi, Conor J Meehan, Alberto Trovato, Antonella Grottola, Elisabetta Giacobazzi, Giulia Fregni Serpini, Sara Tagliazucchi, Anna Fabio, Clotilde Bettua, Roberto Bertorelli, Francesca Frascaro, Veronica De Sanctis, Monica Pecorari, Olivier Jousson, Nicola Segata, Daniela M Cirillo
BACKGROUND: Phylogenetic studies of bacteria have been based so far either on a single gene (usually the 16S rRNA) or on concatenated housekeeping genes. For what concerns the genus Mycobacterium these approaches support the separation of rapidly and slowly growing species and the clustering of most species in well-defined phylogenetic groups. The advent of high-throughput shotgun sequencing leads us to revise conventional taxonomy of mycobacteria on the light of genomic data. For this purpose we investigated 88 newly sequenced species in addition to 60 retrieved from GenBank and used the Average Nucleotide Identity pairwise scores to reconstruct phylogenetic relationships within this genus...
October 11, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/29024173/root-transcriptional-dynamics-induced-by-beneficial-rhizobacteria-and-microbial-immune-elicitors-reveal-signatures-of-adaptation-to-mutualists
#5
Ioannis A Stringlis, Silvia Proietti, Richard Hickman, Marcel C Van Verk, Christos Zamioudis, Corné M J Pieterse
Belowground, microbe-associated molecular patterns (MAMPs) of root-associated microbiota can trigger costly defenses at the expense of plant growth. However, beneficial rhizobacteria, such as Pseudomonas simiae WCS417, promote plant growth and induce systemic resistance without being warded off by local root immune responses. To investigate early root responses that facilitate WCS417 to exert its plant-beneficial functions, we performed time-series RNA-Seq of Arabidopsis roots in response to live WCS417 and compared it to MAMPs flg22(417) (from WCS417), flg22(Pa) (from pathogenic Pseudomonas aeruginosa), and fungal chitin...
October 10, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28990320/non-tuberculosis-mycobacterium-speciation-using-hplc-under-revised-national-tb-control-programme-rntcp-in-india
#6
George Sebastian, Sharath Burugina Nagaraja, Telagi Vishwanatha, Mamatha Voderhobli, Narayana Vijayalakshmi, Prahlad Kumar
AIMS: Non-Tuberculous Mycobacteria (NTM) are ubiquitous in nature. The data on prevalence of NTM under the RNTCP is scarce. Many NTM species have clinical significance, and hence their identification and speciation are important. METHODS AND RESULT: It is a cross-sectional study conducted at the five RNTCP accredited culture and drug susceptibility testing (CDST) laboratory. The culture isolates from AFB positive but Immunochromatographic test negative samples were taken for identification and speciation using HPLC...
October 9, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28938018/genome-wide-identification-of-bacterial-plant-colonization-genes
#7
Benjamin J Cole, Meghan E Feltcher, Robert J Waters, Kelly M Wetmore, Tatiana S Mucyn, Elizabeth M Ryan, Gaoyan Wang, Sabah Ul-Hasan, Meredith McDonald, Yasuo Yoshikuni, Rex R Malmstrom, Adam M Deutschbauer, Jeffery L Dangl, Axel Visel
Diverse soil-resident bacteria can contribute to plant growth and health, but the molecular mechanisms enabling them to effectively colonize their plant hosts remain poorly understood. We used randomly barcoded transposon mutagenesis sequencing (RB-TnSeq) in Pseudomonas simiae, a model root-colonizing bacterium, to establish a genome-wide map of bacterial genes required for colonization of the Arabidopsis thaliana root system. We identified 115 genes (2% of all P. simiae genes) with functions that are required for maximal competitive colonization of the root system...
September 2017: PLoS Biology
https://www.readbyqxmd.com/read/28933031/screening-isolation-and-molecular-identification-of-biodegrading-mycobacteria-from-iranian-ecosystems-and-analysis-of-their-biodegradation-activity
#8
Davood Azadi, Hasan Shojaei, Sina Mobasherizadeh, Abass Daei Naser
Anthropogenic origin pollutants including pesticides, heavy metals, pharmaceuticals and industry chemicals impose many risks to human health and environment and bioremediation has been considered the strategy of choice to reduce the risk of hazardous chemicals. In the current study, we aimed to screen and characterize mycobacteria from the diverse range of Iranian aquatic and terrestrial ecosystems with harsh and unfavorable environmental conditions that can be utilized for biodegradation of target pollutants...
September 20, 2017: AMB Express
https://www.readbyqxmd.com/read/28855835/distribution-of-non-tuberculosis-mycobacteria-strains-from-suspected-tuberculosis-patients-by-heat-shock-protein-65-pcr-rflp
#9
Ali Nour-Neamatollahie, Nayereh Ebrahimzadeh, Seyed Davar Siadat, Farzam Vaziri, Mona Eslami, Abbas Akhavan Sepahi, Sharareh Khanipour, Morteza Masoumi, Fatemeh Sakhaee, Morteza Ghazanfari Jajin, Ahmadreza Bahrmand, Abolfazl Fateh
The genus Mycobacterium contains more than 150 species. Non-tuberculosis mycobacteria (NTM) often cause extrapulmonary and pulmonary disease. Mycobacteria detection at species level is necessary and provides useful information on epidemiology and facilitates successful treatment of patients. This retrospective study aimed to determine the incidence of the NTM isolates and Mycobacterium tuberculosis (Mtb) in clinical specimens collected from Iranian patients during February 2011-December 2013, by PCR-restriction fragment length polymorphism analysis (PRA) of the hsp65 gene...
September 2017: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/28836034/spatio-temporal-study-of-environmental-nontuberculous-mycobacteria-isolated-from-wardha-district-in-central-india
#10
Pranava S Mishra, Pratibha Narang, Rahul Narang, Bidhan Goswami, Deepak K Mendiratta
During the last two decades, nontuberculous mycobacteria (NTM) have gained in importance but there is still a paucity of data, particularly for environmental isolates. We studied, over a period of two years, the spatio-temporal features of NTM isolates obtained from different environmental sources in Wardha district, India. A total of 1398 samples (699 each of soil and water) were tested and 170 (12.2%) yielded NTM isolates, including 123 from soil and 47 from water samples. Out of 170 NTM isolates, 107 (63%) belonged to potentially pathogenic mycobacteria (PPM) and 63 (37%) to the less pathogenic mycobacterial (LPM) group...
August 23, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28809146/mycobacterium-talmoniae-sp-nov-a-slowly-growing-mycobacterium-isolated-from-human-respiratory-samples
#11
Rebecca M Davidson, Mary Ann DeGroote, Jamie L Marola, Sarah Buss, Victoria Jones, Michael R McNeil, Alison G Freifeld, L Elaine Epperson, Nabeeh A Hasan, Mary Jackson, Peter C Iwen, Max Salfinger, Michael Strong
A novel slowly growing, non-chromogenic species of the class Actinobacteria was isolated from a human respiratory sample in Nebraska, USA, in 2012. Analysis of the internal transcribed spacer sequence supported placement into the genus Mycobacterium with high sequence similarity to a previously undescribed strain isolated from a patient respiratory sample from Oregon, USA, held in a collection in Colorado, USA, in 2000. The two isolates were subjected to phenotypic testing and whole genome sequencing and found to be indistinguishable...
August 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28719321/association-of-interferon-%C3%AE-receptor-1-gene-polymorphism-with-nontuberculous-mycobacterial-lung-infection-among-iranian-patients-with-pulmonary-disease
#12
COMPARATIVE STUDY
Poopak Farnia, Jalaledin Ghanavi, Shima Saif, Parissa Farnia, Ali Akbar Velayati
Nontuberculous mycobacteria (NTM) cause significant pulmonary infections in humans. Researchers have reported an association between interferon-gamma receptor-1 (IFN-γR1 or IFNGR1) deficiency and susceptibility to NTM, but the relevance of polymorphism within these genes is not yet clear. In this study, a single nucleotide polymorphism (SNP), T to C, at position-56 in NTM patients with pulmonary disease was investigated. Molecular identification of Mycobacterium isolates was performed with hsp65 genes using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP)...
July 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28688979/remarkable-richness-of-trypanosomes-in-tsetse-flies-glossina-morsitans-morsitans-and-glossina-pallidipes-from-the-gorongosa-national-park-and-niassa-national-reserve-of-mozambique-revealed-by-fluorescent-fragment-length-barcoding-fflb
#13
Herakles A Garcia, Carla M F Rodrigues, Adriana C Rodrigues, Dagmar L Pereira, Carlos L Pereira, Erney P Camargo, P B Hamilton, Marta M G Teixeira
Trypanosomes of African wild ungulates transmitted by tsetse flies can cause human and livestock diseases. However, trypanosome diversity in wild tsetse flies remains greatly underestimated. We employed FFLB (fluorescent fragment length barcoding) for surveys of trypanosomes in tsetse flies (3086) from the Gorongosa National Park (GNP) and Niassa National Reserve (NNR) in Mozambique (MZ), identified as Glossina morsitans morsitans (GNP/NNR=77.6%/90.5%) and Glossina pallidipes (22.4%/9.5%). Trypanosomes were microscopically detected in 8...
July 5, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28527763/aluminium-resistant-plant-growth-promoting-bacteria-induce-overexpression-of-aluminium-stress-related-genes-in-arabidopsis-thaliana-and-increase-the-ginseng-tolerance-against-aluminium-stress
#14
Mohamed El-Agamy Farh, Yeon-Ju Kim, Johan Sukweenadhi, Priyanka Singh, Deok-Chun Yang
Panax ginseng is an important cash crop in the Asian countries due to its pharmaceutical effects, however the plant is exposed to various abiotic stresses, lead to reduction of its quality. One of them is the Aluminum (Al) accumulation. Plant growth promoting bacteria which able to tolerate heavy metals has been considered as a new trend for supporting the growth of many crops in heavy metal occupied areas. In this study, twelve bacteria strains were isolated from rhizosphere of diseased Korean ginseng roots located in Gochang province, Republic of Korea and tested for their ability to grow in Al-embedded broth media...
July 2017: Microbiological Research
https://www.readbyqxmd.com/read/28512635/drug-susceptibility-of-33-reference-strains-of-slowly-growing-mycobacteria-to-19-antimicrobial-agents
#15
Hui Pang, Yi Jiang, Kanglin Wan
Objectives. Slowly growing mycobacteria (SGM) are prevalent worldwide and cause an extensive spectrum of diseases. Methods. In this study, the antimicrobial susceptibility of 33 reference strains of SGM to 19 antimicrobial agents was tested using a modified microdilution method. Results. Cefmetazole (32/33) and azithromycin (32/33) exhibited the highest antimicrobial activity, and dapsone (9/33) exhibited the lowest activity against the tested strains. Cefoxitin (30/33), cefoperazone (28/33), and cefepime (28/33) were effective against a high proportion of strains, and macrolides were also highly effective as well as offering the benefit of convenient oral administration to patients...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28498521/does-drought-stress-modify-the-effects-of-plant-growth-promoting-rhizobacteria-on-an-aboveground-chewing-herbivore
#16
Maite Fernández de Bobadilla, Julia Friman, Nurmi Pangesti, Marcel Dicke, Joop J A van Loon, Ana Pineda
Soil microbes have important effects on the interactions of plants with their environment, by promoting plant growth, inducing resistance to pests or by conferring tolerance to abiotic stress. However, their effects are variable and the factors responsible for this variation are mainly unknown. Our aim was to assess how drought stress modifies the effect of the non-pathogenic rhizobacterium Pseudomonas simiae WCS417r on plant growth and resistance against the generalist leaf-chewing caterpillar Mamestra brassicae...
May 12, 2017: Insect Science
https://www.readbyqxmd.com/read/28438086/feline-leprosy-due-to-candidatus-mycobacterium-tarwinense-further-clinical-and-molecular-characterisation-of-15-previously-reported-cases-and-an-additional-27-cases
#17
Carolyn R O'Brien, Richard Malik, Maria Globan, George Reppas, Christina McCowan, Janet A Fyfe
OBJECTIVES: This paper, the first in a series of three on 'feline leprosy', provides a detailed description of disease referable to Candidatus 'Mycobacterium tarwinense', the most common cause of feline leprosy in Victoria, Australia. METHODS: Cases were sourced retrospectively and prospectively for this observational study, describing clinical, geographical and molecular microbiological data for cats definitively diagnosed with Candidatus 'M tarwinense' infection...
May 2017: Journal of Feline Medicine and Surgery
https://www.readbyqxmd.com/read/28334335/antagonism-between-two-root-associated-beneficial-pseudomonas-strains-does-not-affect-plant-growth-promotion-and-induced-resistance-against-a-leaf-chewing-herbivore
#18
Nurmi Pangesti, Simon Vandenbrande, Ana Pineda, Marcel Dicke, Jos M Raaijmakers, Joop J A Van Loon
Plant growth-promoting microbes residing on the roots may cooperate or compete, thereby affecting their collective benefit to the host plant. Pseudomonas simiae WCS417r (formerly known as P. fluorescens WCS417r) and Pseudomonas fluorescens SS101 are well known for their ability to induce systemic resistance in Arabidopsis. Here, we evaluate how these species interact on the roots of Arabidopsis thaliana Col-0 and how their co-inoculation affects plant defense to the leaf-chewing herbivore Mamestra brassicae and plant growth promotion...
April 1, 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/28331086/nectin-4-interactions-govern-measles-virus-virulence-in-a-new-model-of-pathogenesis-the-squirrel-monkey-saimiri-sciureus
#19
Sébastien Delpeut, Bevan Sawatsky, Xiao-Xiang Wong, Marie Frenzke, Roberto Cattaneo, Veronika von Messling
In addition to humans, only certain nonhuman primates are naturally susceptible to measles virus (MeV) infection. Disease severity is species dependent, ranging from mild to moderate for macaques to severe and even lethal for certain New World monkey species. To investigate if squirrel monkeys (Saimiri sciureus), which are reported to develop a course of disease similar to humans, may be better suited than macaques for the identification of virulence determinants or the evaluation of therapeutics, we infected them with a green fluorescent protein-expressing MeV...
June 1, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28330892/in-vitro-susceptibility-testing-of-tedizolid-against-nontuberculous-mycobacteria
#20
Barbara A Brown-Elliott, Richard J Wallace
Tedizolid is a new oxazolidinone with improved in vitro and intracellular potency against Mycobacterium tuberculosis, including multidrug-resistant strains, and some species of nontuberculous mycobacteria (NTM) compared with that of linezolid. Using the current Clinical and Laboratory Standards Institute (CLSI)-recommended method of broth microdilution, susceptibility testing of 170 isolates of rapidly growing mycobacteria showed equivalent or lower (1- to 8-fold) MIC50 and/or MIC90 values for tedizolid compared with that for linezolid...
June 2017: Journal of Clinical Microbiology
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