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Shradha Subedi, Fanrong Kong, Peter Jelfs, Timothy J Gray, Meng Xiao, Vitali Sintchenko, Sharon C-A Chen
Accurate identification of slowly growing nontuberculous mycobacteria (SG-NTM) of clinical significance remains problematic. This study evaluated a novel method of SG-NTM identification by amplification of the mycobacterial 16S-23S rRNA internal transcribed spacer (ITS) region followed by resolution of amplified fragments by sequencer-based capillary gel electrophoresis (SCGE). Fourteen American Type Culture Collection (ATCC) strains and 103 clinical/environmental isolates (total n = 24 species) of SG-NTM were included...
2016: PloS One
D Kušar, U Zajc, V Jenčič, M Ocepek, J Higgins, M Žolnir-Dovč, M Pate
Fish are commonly infected with non-tuberculous mycobacteria (NTM), which should be regarded as potential pathogens when handling aquarium fish and equipment. This study examined 107 aquarium fish from pet shops. Cultivation of the fish samples using different selective media was conducted for identification of NTM. Isolates were identified using the GenoType Mycobacterium common mycobacteria and additional species assays, sequencing of the 16S rRNA and rpoB genes, and real-time PCR assay for identification of Mycobacterium (M...
October 17, 2016: Journal of Fish Diseases
Ivan Malík, Jozef Csöllei, Josef Jampílek, Lukáš Stanzel, Iveta Zadražilová, Jan Hošek, Šárka Pospíšilová, Alois Čížek, Aidan Coffey, Jim O'Mahony
This research was focused on in silico characterization and in vitro biological testing of the series of the compounds carrying a N-arylpiperazine moiety. The in silico investigation was based on the prediction of electronic, steric and lipohydrophilic features. The molecules were screened against Mycobacterium avium subsp. paratuberculosis CIT03, M. smegmatis ATCC 700084, M. kansasii DSM 44162, M. marinum CAMP 5644, Staphylococcus aureus ATCC 29213, methicillin-resistant S. aureus 63718, Escherichia coli ATCC 25922, Enterococcus faecalis ATCC 29212, Candida albicans CCM 8261, C...
2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Wei Song, Li Liu, Hongzhou Lu
Obstract: To identify Mycobacteria isolated from AIDS patients and to evaluate the diagnostic value of MBP64 antigen Colloidal Gold Diagnostic Kit in distinguishing Mycobacterium tuberculosis(MTB) and Nontuberculous mycobacteria (NTM). Methods: A total of 140 Mycobacteria isolates from AIDS patients were collected and identified by using 16SrDNA PCR assay as well as immune colloidal gold technique (MBP64 antigen Colloidal Gold Diagnostic Kit). The sensitivity and specificity of Colloidal Gold method was analyzed...
May 25, 2016: Zhejiang da Xue Xue Bao. Yi Xue Ban, Journal of Zhejiang University. Medical Sciences
Song Wei, Liu Li, L U Hongzhou
Obstract: To identify Mycobacteria isolated from AIDS patients and to evaluate the diagnostic value of MBP64 antigen Colloidal Gold Diagnostic Kit in distinguishing Mycobacterium tuberculosis(MTB) and Nontuberculous mycobacteria (NTM). Methods: A total of 140 Mycobacteria isolates from AIDS patients were collected and identified by using 16SrDNA PCR assay as well as immune colloidal gold technique (MBP64 antigen Colloidal Gold Diagnostic Kit). The sensitivity and specificity of Colloidal Gold method was analyzed...
May 25, 2016: Zhejiang da Xue Xue Bao. Yi Xue Ban, Journal of Zhejiang University. Medical Sciences
Tomas Gonec, Sarka Pospisilova, Lucie Holanova, Josef Stranik, Aneta Cernikova, Valeria Pudelkova, Jiri Kos, Michal Oravec, Peter Kollar, Alois Cizek, Josef Jampilek
Series of thirteen 1-[(2-chlorophenyl)carbamoyl]naphthalen-2-yl carbamates and thirteen 1-[(2-nitrophenyl)carbamoyl]naphthalen-2-yl carbamates with alkyl/cycloalkyl/arylalkyl chains were prepared and characterized. Primary in vitro screening of the synthesized compounds was performed against Staphylococcus aureus, two methicillin-resistant S. aureus strains, Mycobacterium marinum, and M. kansasii. 1-[(2-Chlorophenyl)carbamoyl]naphthalen-2-yl ethylcarbamate and 1-[(2-nitrophenyl)carbamoyl]naphthalen-2-yl ethylcarbamate showed antistaphylococcal (MICs = 42 µM against MRSA) and antimycobacterial (MICs = 21 µM) activity against the tested strains comparable with or higher than that of the standards ampicillin and isoniazid...
2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Jana P Lovell, Christa S Zerbe, Kenneth N Olivier, Reginald J Claypool, Cathleen Frein, Victoria L Anderson, Alexandra F Freeman, Steven M Holland
RATIONALE: Mycobacterium kansasii usually causes chronic pulmonary infections in immunocompetent patients. In contrast, disseminated M. kansasii disease is commonly associated with advanced HIV infection, but is reported infrequently in other immunocompromised patients. OBJECTIVES: To identify common clinical manifestations and potential risk factors for M. kansasii infection in patients with GATA2 deficiency. METHODS: We reviewed M. kansasii disease associated with GATA2 deficiency at one institution and disease associated with primary and other immunodeficiencies reported in the literature...
September 8, 2016: Annals of the American Thoracic Society
Kate Buchacz, Bryan Lau, Yuezhou Jing, Ronald Bosch, Alison G Abraham, M John Gill, Michael J Silverberg, James J Goedert, Timothy R Sterling, Keri N Althoff, Jeffrey N Martin, Greer Burkholder, Neel Gandhi, Hasina Samji, Pragna Patel, Anita Rachlis, Jennifer E Thorne, Sonia Napravnik, Keith Henry, Angel Mayor, Kelly Gebo, Stephen J Gange, Richard D Moore, John T Brooks
BACKGROUND: There are few recent data on the rates of AIDS-defining opportunistic infections (OIs) among human immunodeficiency virus (HIV)-infected patients in care in the United States and Canada. METHODS: We studied HIV-infected participants in 16 cohorts in the North American AIDS Cohort Collaboration on Research and Design (NA-ACCORD) during 2000-2010. After excluding 16 737 (21%) with any AIDS-defining clinical events documented before NA-ACCORD enrollment, we analyzed incident OIs among the remaining 63 541 persons, most of whom received antiretroviral therapy during the observation...
September 15, 2016: Journal of Infectious Diseases
Tomas Gonec, Sarka Pospisilova, Tereza Kauerova, Jiri Kos, Jana Dohanosova, Michal Oravec, Peter Kollar, Aidan Coffey, Tibor Liptaj, Alois Cizek, Josef Jampilek
A series of nineteen N-(alkoxyphenyl)-2-hydroxynaphthalene-1-carboxamides and a series of their nineteen positional isomers N-(alkoxyphenyl)-1-hydroxynaphthalene-2-carboxamides were prepared and characterized. Primary in vitro screening of all the synthesized compounds was performed against Mycobacterium tuberculosis H37Ra, M. kansasii and M. smegmatis. Screening of the cytotoxicity of the compounds was performed using human monocytic leukemia THP-1 cells. Some of the tested compounds showed antimycobacterial activity comparable with or higher than that of rifampicin...
2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Yoshiya Tsunoda, Akimasa Sekine, Yuki Sumazaki, Toru Tanaka, Shih-Yuan Lin, Hiroyuki Takoi, Kenji Hayashihara, Ataru Moriya, Takefumi Saito
INTRODUCTION: Mycobacterium abscessus pulmonary disease is common in patients with bronchiectasis. However, the underlying disease that is more likely to be present in patients with M. abscessus pulmonary disease remains poorly understood. METHOD: From 2001 through 2010, all patients, whose sputum or bronchoscopic lavage cultures yielded M. abscessus, were included in the study. RESULTS: Among the 11 patients included (male/female: 4/7), 4 male patients had a history of smoking...
April 2016: Kekkaku: [Tuberculosis]
N Ose, H Maeda, Y Takeuchi, Y Susaki, Y Kobori, S Taniguchi, R Maekura
BACKGROUND: In some patients, non-tuberculous mycobacteria (NTM) infections manifest in solitary nodules (solitary nodular [SN] type) generally caused by Mycobacterium avium complex (MAC). In patients treated using surgical resection, the American Thoracic Society guidelines state that postoperative chemotherapy is not necessary in the absence of lesions, although there have been a few reports of such cases. METHODS: Twenty-eight patients diagnosed with NTM who underwent solitary pulmonary nodule resection at Toneyama Hospital, Osaka, Japan, between January 2000 and October 2012 were enrolled...
August 2016: International Journal of Tuberculosis and Lung Disease
Nomakorinte Gcebe, Anita Michel, Nicolaas C Gey van Pittius, Victor Rutten
The Esx and PE/PPE families of proteins are among the most immunodominant mycobacterial antigens and have thus been the focus of research to develop vaccines and immunological tests for diagnosis of bovine and human tuberculosis, mainly caused by Mycobacterium bovis and Mycobacterium tuberculosis, respectively. In non-tuberculous mycobacteria (NTM), multiple copies of genes encoding homologous proteins have mainly been identified in pathogenic Mycobacterium species phylogenically related to Mycobacterium tuberculosis and Mycobacterium bovis...
2016: Frontiers in Microbiology
Nyasha Chin'ombe, Boniface Muzividzi, Ellen Munemo, Pasipanodya Nziramasanga
BACKGROUND: Several nontuberculous mycobacteria (NTM) were previously isolated from diverse environments such as water, soil, sewage, food and animals. Some of these NTM are now known to be opportunistic pathogens of humans. OBJECTIVE: The main purpose of the study was to identify NTM isolates stored at the National Microbiology Reference Laboratory (NMRL) and were previously isolated from humans during a national tuberculosis (TB) survey. METHODS: Pure NTM cultures already isolated from human sputum samples during the national TB survey were retrieved from the NMRL and used for this study...
2016: Open Microbiology Journal
Bahram Nasr Esfahani, Sharareh Moghim, Hajieh Ghasemian Safaei, Mohsen Moghoofei, Mansour Sedighi, Shima Hadifar
BACKGROUND: Taxonomic and phylogenetic studies of Mycobacterium species have been based around the 16sRNA gene for many years. However, due to the high strain similarity between species in the Mycobacterium genus (94.3% - 100%), defining a valid phylogenetic tree is difficult; consequently, its use in estimating the boundaries between species is limited. The sequence of the rpoB gene makes it an appropriate gene for phylogenetic analysis, especially in bacteria with limited variation...
April 2016: Jundishapur Journal of Microbiology
Dominik Strapagiel, Paulina Borówka, Błażej Marciniak, Zofia Bakuła, Jakko van Ingen, Aleksandra Safianowska, Anna Brzostek, Jarosław Dziadek, Tomasz Jagielski
Mycobacterium kansasii belongs to the nontuberculous mycobacteria (NTM) and causes opportunistic infections with both pulmonary and extrapulmonary manifestations. Here, we report the draft genome sequences of six environmental M. kansasii strains, designated 1010001495 (type I), 1010001469 (type II), 1010001468 (type III), 1010001458 (type IV), 1010001454 (type V), and 1010001493 (type V), originally isolated in five different European countries.
2016: Genome Announcements
Nasstasja Wassilew, Harald Hoffmann, Claire Andrejak, Christoph Lange
Non-tuberculous mycobacteria (NTM) include more than 160 ubiquitous, environmental, acid-fast-staining bacterial species, some of which may cause disease in humans. Chronic pulmonary infection is the most common clinical manifestation. Although patients suffering from chronic lung diseases are particularly susceptible to NTM pulmonary disease, many affected patients have no apparent risk factors. Host and pathogen factors leading to NTM pulmonary disease are not well understood and preventive therapies are lacking...
2016: Respiration; International Review of Thoracic Diseases
Kazutaka Izawa, Seigo Kitada
OBJECTIVE: The incidence rate of nontuberculous mycobacterial (NTM) infection has been increasing globally in recent years. However, reports of osteoarticular NTM infection are relatively rare. We report the characteristic clinical features of patients with osteoarticular NTM infection. PATIENTS AND METHODS: We examined 14 patients with osteoarticular NTM infection (mean age, 68 years) were treated in our hospital in the 20 years between 1995 and 2015. RESULTS: The rate of osteoarticular NTM infection in whole osteoarticular infection during the same period in our hospital was 2...
January 2016: Kekkaku: [Tuberculosis]
Yong-Soo Kwon, Won-Jung Koh
Nontuberculous mycobacteria (NTM) are ubiquitous organisms; their isolation from clinical specimens does not always indicate clinical disease. The incidence of NTM lung diseases has been increasing worldwide. Although the geographic diversity of NTM species is well known, Mycobacterium avium complex (MAC), M. abscessus complex (MABC), and M. kansasii are the most commonly encountered and important etiologic organisms. Two distinct types of NTM lung diseases have been reported, namely fibrocavitary and nodular bronchiectatic forms...
May 2016: Journal of Korean Medical Science
L L Liu, S J Chen, H Long, C Wang, T Y Cao, Z X Hu, D Wu
OBJECTIVE: To establish a multiplex polymerase chain reaction (mPCR) method with high sensitivity and specificity for rapid detection of common nontuberculous mycobacterium(NTM) infection in the hand. METHODS: Application of primer design software to the mycobacterium marinum, mycobacterium avium, mycobacterium kansasii and mycobacterium fortuitum, the specific gene sequences were used to design construction of multiplex PCR and detection of DNA from the non tuberculous mycobacterial standard strains of each bacterium of single PCR and multiplex DNA accuracy and sequence contrast evaluation to verify the specificity of multiple PCR primers...
April 12, 2016: Zhonghua Yi Xue za Zhi [Chinese medical journal]
Fabiane N Riello, Rebecca T S Brígido, Sergio Araújo, Tomaz A Moreira, Luiz Ricardo Goulart, Isabela M B Goulart
BACKGROUND: The establishment of therapeutic regimens for mycobacteriosis depends on the accurate identification of Mycobacterium species, and misdiagnosis can result in inappropriate treatment and increased mortality of patients. Differential diagnosis among Mycobacterium species has been made by conventional phenotypic and biochemical tests after a long culture period. Specialized molecular diagnostics of mycobacteria allows rapid detection and species identification; however, such tests are not available in public health programs...
2016: BMC Infectious Diseases
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