Yasmin Castillos das Neves, Ana Julia Reis, Marcos Alaniz Rodrigues, Erica Chimara, Maria Cristina da Silva Lourenço, Jacques Fountain, Ivy Bastos Ramis, Andrea von Groll, Yulia Gerasimova, Kyle H Rohde, Pedro Eduardo Almeida da Silva
Tuberculosis (TB) and infectious diseases caused by non-tuberculous mycobacteria (NTM) are global concerns. The development of a rapid and accurate diagnostic method, capable of detecting and identifying different mycobacteria species, is crucial. We propose a molecular approach, the BiDz-TB/NTM, based on the use of binary deoxyribozyme (BiDz) sensors for the detection of Mycobacterium tuberculosis (Mtb) and NTM of clinical interest. A panel of DNA samples was used to evaluate Mtb-BiDz, Mycobacterium abscessus / Mycobacterium chelonae -BiDz, Mycobacterium avium -BiDz, Mycobacterium intracellulare / Mycobacterium chimaera -BiDz, and Mycobacterium kansasii -BiDz sensors in terms of specificity, sensitivity, accuracy, and limit of detection...
April 23, 2024: Microbiology Spectrum