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https://www.readbyqxmd.com/read/27911922/nucleic-acid-amplification-testing-and-sequencing-combined-with-acid-fast-staining-in-needle-biopsy-lung-tissues-for-the-diagnosis-of-smear-negative-pulmonary-tuberculosis
#1
Faming Jiang, Weiwei Huang, Ye Wang, Panwen Tian, Xuerong Chen, Zongan Liang
BACKGROUND: Smear-negative pulmonary tuberculosis (PTB) is common and difficult to diagnose. In this study, we investigated the diagnostic value of nucleic acid amplification testing and sequencing combined with acid-fast bacteria (AFB) staining of needle biopsy lung tissues for patients with suspected smear-negative PTB. METHODS: Patients with suspected smear-negative PTB who underwent percutaneous transthoracic needle biopsy between May 1, 2012, and June 30, 2015, were enrolled in this retrospective study...
2016: PloS One
https://www.readbyqxmd.com/read/27851430/1795-life-threatening-immune-reconstitution-inflammatory-syndrome-due-to-m-kansasii-in-an-aids-patient
#2
Naseem Alavian, Simon Yau, Nicola Hanania
No abstract text is available yet for this article.
December 2016: Critical Care Medicine
https://www.readbyqxmd.com/read/27808247/clinical-significance-of-nontuberculous-mycobacteria-isolated-from-respiratory-specimens-in-a-chinese-tuberculosis-tertiary-care-center
#3
Hongfei Duan, Xiqin Han, Qingfeng Wang, Jing Wang, Jun Wang, Naihui Chu, Hairong Huang
The clinical relevance of non-tuberculous mycobacteria (NTM) has been reported to be different dramatically by species or by regions, however, no such evaluation has been performed in China.A retrospective study was performed in Beijing Chest Hospital. All the NTM strains isolated from respiratory specimens in the past 5 years, and patients' clinical records (symptoms and radiographic information etc.) were investigated. The clinical relevance was evaluated according to the criteria recommended by the American Thoracic society...
November 3, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27801810/novel-halogenated-pyrazine-based-chalcones-as-potential-antimicrobial-drugs
#4
Marta Kucerova-Chlupacova, Veronika Vyskovska-Tyllova, Lenka Richterova-Finkova, Jiri Kunes, Vladimir Buchta, Marcela Vejsova, Pavla Paterova, Lucia Semelkova, Ondrej Jandourek, Veronika Opletalova
Chalcones, i.e., compounds with the chemical pattern of 1,3-diphenylprop-2-en-1-ones, exert a wide range of bio-activities, e.g., antioxidant, anti-inflammatory, anticancer, anti-infective etc. Our research group has been focused on pyrazine analogues of chalcones; several series have been synthesized and tested in vitro on antifungal and antimycobacterial activity. The highest potency was exhibited by derivatives with electron withdrawing groups (EWG) in positions 2 and 4 of the ring B. As halogens also have electron withdrawing properties, novel halogenated derivatives were prepared by Claisen-Schmidt condensation...
October 27, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27788025/a-laboratory-based-analysis-of-nontuberculous-mycobacterial-lung-disease-in-japan-from-2012-to-2013
#5
Kozo Morimoto, Naoki Hasegawa, Kiyohiko Izumi, Ho Namkoong, Kazuhiro Uchimura, Takashi Yoshiyama, Yoshihiko Hoshino, Atsuyuki Kurashima, Jun Sokunaga, Shunsuke Shibuya, Masahiro Shimojima, Manabu Ato, Satoshi Mitarai
RATIONALE: Since 2010, studies that utilize mycobacterial examination results have been widely used to survey nontuberculous mycobacterial (NTM) lung disease. OBJECTIVES: Our study aims to reveal the clinical and epidemiological status of NTM lung disease in Japan. METHODS: All data on the isolation and identification of mycobacteria in 2012 and 2013 were obtained from three dominant commercial laboratories in Japan. Pulmonary disease were defined based on the bacteriological diagnostic criteria issued by the American Thoracic Society and the Infectious Diseases Society of America (ATS/IDSA)...
October 27, 2016: Annals of the American Thoracic Society
https://www.readbyqxmd.com/read/27777490/the-importance-of-computed-tomography-of-the-chest-in-cases-of-suspected-infection-with-nontuberculous-mycobacteria-mycobacterium-kansasii
#6
Miriam Menna Barreto, Rosana Souza Rodrigues
No abstract text is available yet for this article.
July 2016: Radiologia Brasileira
https://www.readbyqxmd.com/read/27777472/pulmonary-infection-caused-by-mycobacterium-kansasii-findings-on-computed-tomography-of-the-chest
#7
Roberto Mogami, Telma Goldenberg, Patricia Gomes Cytrangulo de Marca, Fernanda Carvalho de Queiroz Mello, Agnaldo José Lopes
OBJECTIVE: To describe the main tomography findings in patients diagnosed with pulmonary infection caused by Mycobacterium kansasii. MATERIALS AND METHODS: Retrospective study of computed tomography scans of 19 patients with pulmonary infection by M. kansasii. RESULTS: Of the 19 patients evaluated, 10 (52.6%) were male and 9 (47.4%) were female. The mean age of the patients was 58 years (range, 33-76 years). Computed tomography findings were as follows: architectural distortion, in 17 patients (89...
July 2016: Radiologia Brasileira
https://www.readbyqxmd.com/read/27749897/16s-23s-internal-transcribed-spacer-region-pcr-and-sequencer-based-capillary-gel-electrophoresis-has-potential-as-an-alternative-to-high-performance-liquid-chromatography-for-identification-of-slowly-growing-nontuberculous-mycobacteria
#8
Shradha Subedi, Fanrong Kong, Peter Jelfs, Timothy J Gray, Meng Xiao, Vitali Sintchenko, Sharon C-A Chen
Accurate identification of slowly growing nontuberculous mycobacteria (SG-NTM) of clinical significance remains problematic. This study evaluated a novel method of SG-NTM identification by amplification of the mycobacterial 16S-23S rRNA internal transcribed spacer (ITS) region followed by resolution of amplified fragments by sequencer-based capillary gel electrophoresis (SCGE). Fourteen American Type Culture Collection (ATCC) strains and 103 clinical/environmental isolates (total n = 24 species) of SG-NTM were included...
2016: PloS One
https://www.readbyqxmd.com/read/27747884/mycobacteria-in-aquarium-fish-results-of-a-3-year-survey-indicate-caution-required-in-handling-pet-shop-fish
#9
D Kušar, U Zajc, V Jenčič, M Ocepek, J Higgins, M Žolnir-Dovč, M Pate
Fish are commonly infected with non-tuberculous mycobacteria (NTM), which should be regarded as potential pathogens when handling aquarium fish and equipment. This study examined 107 aquarium fish from pet shops. Cultivation of the fish samples using different selective media was conducted for identification of NTM. Isolates were identified using the GenoType Mycobacterium common mycobacteria and additional species assays, sequencing of the 16S rRNA and rpoB genes, and real-time PCR assay for identification of Mycobacterium (M...
October 17, 2016: Journal of Fish Diseases
https://www.readbyqxmd.com/read/27681720/the-structure-antimicrobial-activity-relationships-of-a-promising-class-of-the-compounds-containing-the-n-arylpiperazine-scaffold
#10
Ivan Malík, Jozef Csöllei, Josef Jampílek, Lukáš Stanzel, Iveta Zadražilová, Jan Hošek, Šárka Pospíšilová, Alois Čížek, Aidan Coffey, Jim O'Mahony
This research was focused on in silico characterization and in vitro biological testing of the series of the compounds carrying a N-arylpiperazine moiety. The in silico investigation was based on the prediction of electronic, steric and lipohydrophilic features. The molecules were screened against Mycobacterium avium subsp. paratuberculosis CIT03, M. smegmatis ATCC 700084, M. kansasii DSM 44162, M. marinum CAMP 5644, Staphylococcus aureus ATCC 29213, methicillin-resistant S. aureus 63718, Escherichia coli ATCC 25922, Enterococcus faecalis ATCC 29212, Candida albicans CCM 8261, C...
2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27677657/-identification-of-mycobacteria-isolated-from-aids-patients
#11
Wei Song, Li Liu, Hongzhou Lu
Obstract: To identify Mycobacteria isolated from AIDS patients and to evaluate the diagnostic value of MBP64 antigen Colloidal Gold Diagnostic Kit in distinguishing Mycobacterium tuberculosis(MTB) and Nontuberculous mycobacteria (NTM). Methods: A total of 140 Mycobacteria isolates from AIDS patients were collected and identified by using 16SrDNA PCR assay as well as immune colloidal gold technique (MBP64 antigen Colloidal Gold Diagnostic Kit). The sensitivity and specificity of Colloidal Gold method was analyzed...
May 25, 2016: Zhejiang da Xue Xue Bao. Yi Xue Ban, Journal of Zhejiang University. Medical Sciences
https://www.readbyqxmd.com/read/27651187/-identification-of-mycobacteria-isolated-from-aids-patients
#12
Song Wei, Liu Li, L U Hongzhou
Obstract: To identify Mycobacteria isolated from AIDS patients and to evaluate the diagnostic value of MBP64 antigen Colloidal Gold Diagnostic Kit in distinguishing Mycobacterium tuberculosis(MTB) and Nontuberculous mycobacteria (NTM). Methods: A total of 140 Mycobacteria isolates from AIDS patients were collected and identified by using 16SrDNA PCR assay as well as immune colloidal gold technique (MBP64 antigen Colloidal Gold Diagnostic Kit). The sensitivity and specificity of Colloidal Gold method was analyzed...
May 25, 2016: Zhejiang da Xue Xue Bao. Yi Xue Ban, Journal of Zhejiang University. Medical Sciences
https://www.readbyqxmd.com/read/27617988/synthesis-and-antimicrobial-evaluation-of-1-2-substituted-phenyl-carbamoyl-naphthalen-2-yl-carbamates
#13
Tomas Gonec, Sarka Pospisilova, Lucie Holanova, Josef Stranik, Aneta Cernikova, Valeria Pudelkova, Jiri Kos, Michal Oravec, Peter Kollar, Alois Cizek, Josef Jampilek
Series of thirteen 1-[(2-chlorophenyl)carbamoyl]naphthalen-2-yl carbamates and thirteen 1-[(2-nitrophenyl)carbamoyl]naphthalen-2-yl carbamates with alkyl/cycloalkyl/arylalkyl chains were prepared and characterized. Primary in vitro screening of the synthesized compounds was performed against Staphylococcus aureus, two methicillin-resistant S. aureus strains, Mycobacterium marinum, and M. kansasii. 1-[(2-Chlorophenyl)carbamoyl]naphthalen-2-yl ethylcarbamate and 1-[(2-nitrophenyl)carbamoyl]naphthalen-2-yl ethylcarbamate showed antistaphylococcal (MICs = 42 µM against MRSA) and antimycobacterial (MICs = 21 µM) activity against the tested strains comparable with or higher than that of the standards ampicillin and isoniazid...
2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27607353/mediastinal-and-disseminated-mycobacterium-kansasii-disease-in-gata2-deficiency
#14
Jana P Lovell, Christa S Zerbe, Kenneth N Olivier, Reginald J Claypool, Cathleen Frein, Victoria L Anderson, Alexandra F Freeman, Steven M Holland
RATIONALE: Mycobacterium kansasii usually causes chronic pulmonary infections in immunocompetent patients. In contrast, disseminated M. kansasii disease is commonly associated with advanced HIV infection, but is reported infrequently in other immunocompromised patients. OBJECTIVES: To identify common clinical manifestations and potential risk factors for M. kansasii infection in patients with GATA2 deficiency. METHODS: We reviewed M. kansasii disease associated with GATA2 deficiency at one institution and disease associated with primary and other immunodeficiencies reported in the literature...
September 8, 2016: Annals of the American Thoracic Society
https://www.readbyqxmd.com/read/27559122/incidence-of-aids-defining-opportunistic-infections-in-a-multicohort-analysis-of-hiv-infected-persons-in-the-united-states-and-canada-2000-2010
#15
Kate Buchacz, Bryan Lau, Yuezhou Jing, Ronald Bosch, Alison G Abraham, M John Gill, Michael J Silverberg, James J Goedert, Timothy R Sterling, Keri N Althoff, Jeffrey N Martin, Greer Burkholder, Neel Gandhi, Hasina Samji, Pragna Patel, Anita Rachlis, Jennifer E Thorne, Sonia Napravnik, Keith Henry, Angel Mayor, Kelly Gebo, Stephen J Gange, Richard D Moore, John T Brooks
BACKGROUND: There are few recent data on the rates of AIDS-defining opportunistic infections (OIs) among human immunodeficiency virus (HIV)-infected patients in care in the United States and Canada. METHODS: We studied HIV-infected participants in 16 cohorts in the North American AIDS Cohort Collaboration on Research and Design (NA-ACCORD) during 2000-2010. After excluding 16 737 (21%) with any AIDS-defining clinical events documented before NA-ACCORD enrollment, we analyzed incident OIs among the remaining 63 541 persons, most of whom received antiretroviral therapy during the observation...
September 15, 2016: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/27537867/n-alkoxyphenylhydroxynaphthalenecarboxamides-and-their-antimycobacterial-activity
#16
Tomas Gonec, Sarka Pospisilova, Tereza Kauerova, Jiri Kos, Jana Dohanosova, Michal Oravec, Peter Kollar, Aidan Coffey, Tibor Liptaj, Alois Cizek, Josef Jampilek
A series of nineteen N-(alkoxyphenyl)-2-hydroxynaphthalene-1-carboxamides and a series of their nineteen positional isomers N-(alkoxyphenyl)-1-hydroxynaphthalene-2-carboxamides were prepared and characterized. Primary in vitro screening of all the synthesized compounds was performed against Mycobacterium tuberculosis H37Ra, M. kansasii and M. smegmatis. Screening of the cytotoxicity of the compounds was performed using human monocytic leukemia THP-1 cells. Some of the tested compounds showed antimycobacterial activity comparable with or higher than that of rifampicin...
2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27530020/-mycobacterium-abscessus-pulmonary-disease-important-pathogen-involved-in-microbial-substitution-during-the-treatment-of-non-abscessus-mycobacterial-disease
#17
Yoshiya Tsunoda, Akimasa Sekine, Yuki Sumazaki, Toru Tanaka, Shih-Yuan Lin, Hiroyuki Takoi, Kenji Hayashihara, Ataru Moriya, Takefumi Saito
INTRODUCTION: Mycobacterium abscessus pulmonary disease is common in patients with bronchiectasis. However, the underlying disease that is more likely to be present in patients with M. abscessus pulmonary disease remains poorly understood. METHOD: From 2001 through 2010, all patients, whose sputum or bronchoscopic lavage cultures yielded M. abscessus, were included in the study. RESULTS: Among the 11 patients included (male/female: 4/7), 4 male patients had a history of smoking...
April 2016: Kekkaku: [Tuberculosis]
https://www.readbyqxmd.com/read/27393550/solitary-pulmonary-nodules-due-to-non-tuberculous-mycobacteriosis-among-28-resected-cases
#18
N Ose, H Maeda, Y Takeuchi, Y Susaki, Y Kobori, S Taniguchi, R Maekura
BACKGROUND: In some patients, non-tuberculous mycobacteria (NTM) infections manifest in solitary nodules (solitary nodular [SN] type) generally caused by Mycobacterium avium complex (MAC). In patients treated using surgical resection, the American Thoracic Society guidelines state that postoperative chemotherapy is not necessary in the absence of lesions, although there have been a few reports of such cases. METHODS: Twenty-eight patients diagnosed with NTM who underwent solitary pulmonary nodule resection at Toneyama Hospital, Osaka, Japan, between January 2000 and October 2012 were enrolled...
August 2016: International Journal of Tuberculosis and Lung Disease
https://www.readbyqxmd.com/read/27375559/comparative-genomics-and-proteomic-analysis-of-four-non-tuberculous-mycobacterium-species-and-mycobacterium-tuberculosis-complex-occurrence-of-shared-immunogenic-proteins
#19
Nomakorinte Gcebe, Anita Michel, Nicolaas C Gey van Pittius, Victor Rutten
The Esx and PE/PPE families of proteins are among the most immunodominant mycobacterial antigens and have thus been the focus of research to develop vaccines and immunological tests for diagnosis of bovine and human tuberculosis, mainly caused by Mycobacterium bovis and Mycobacterium tuberculosis, respectively. In non-tuberculous mycobacteria (NTM), multiple copies of genes encoding homologous proteins have mainly been identified in pathogenic Mycobacterium species phylogenically related to Mycobacterium tuberculosis and Mycobacterium bovis...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27335623/molecular-identification-of-nontuberculous-mycobacteria-in-humans-in-zimbabwe-using-16s-ribosequencing
#20
Nyasha Chin'ombe, Boniface Muzividzi, Ellen Munemo, Pasipanodya Nziramasanga
BACKGROUND: Several nontuberculous mycobacteria (NTM) were previously isolated from diverse environments such as water, soil, sewage, food and animals. Some of these NTM are now known to be opportunistic pathogens of humans. OBJECTIVE: The main purpose of the study was to identify NTM isolates stored at the National Microbiology Reference Laboratory (NMRL) and were previously isolated from humans during a national tuberculosis (TB) survey. METHODS: Pure NTM cultures already isolated from human sputum samples during the national TB survey were retrieved from the NMRL and used for this study...
2016: Open Microbiology Journal
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