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https://www.readbyqxmd.com/read/29207758/mycobacterium-fortuitum-infection-at-umbilical-hernioplasty-site
#1
Bharti Chogtu, Daliparty Vasudev Malik, Rahul Magazine, Vishnu Prasad Shenoy
Non Tuberculous Mycobacteria (NTM) are a group of rapidly growing mycobacteria and are generally considered to be of low virulence. Of late, there has been an increase in incidence of infections due to these organisms. Among them, Mycobacterium fortuitum, M. chelonae and M. abscessus are the common species which have been identified. Though they are occasionally implicated in pulmonary infections, NTM are very commonly associated with cutaneous infections, especially surgical site infections. Identification of NTM infection at such sites should be suspected when there is delayed healing of the wound...
September 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/29121135/sporotrichoid-pattern-of-mycobacterium-chelonae-abscessus-infection
#2
Jessica L Boulavsky, Holly M Wright, Paul M Rodriguez-Waitkus, Andrew R DiNardo, Laila E Woc-Colburn, Harry Dao
No abstract text is available yet for this article.
September 2017: Cutis; Cutaneous Medicine for the Practitioner
https://www.readbyqxmd.com/read/29111994/identification-of-the-infection-source-of-an-outbreak-of-mycobacterium-chelonae-keratitis-after-laser-in-situ-keratomileusis
#3
Heloisa Nascimento, Cristina Viana-Niero, Christiane Lourenço Nogueira, Paulo José Martins Bispo, Fernando Pinto, Camila de Paula Pereira Uzam, Cristianne Kayoko Matsumoto, Antônia Maria Oliveira Machado, Sylvia Cardoso Leão, Ana Luisa Höfling-Lima, Denise de Freitas
PURPOSE: Nontuberculous mycobacteria keratitis is a rare but challenging complication of laser in situ keratomileusis (LASIK). This study was conducted to determine the source(s) of infection in a cluster of cases of keratitis after LASIK and to describe this outbreak and patients' outcomes. METHODS: In this retrospective, case series, single-center study, 86 patients were included who underwent LASIK or photorefractive keratectomy between December 2011 and February 2012...
November 2, 2017: Cornea
https://www.readbyqxmd.com/read/29109708/differential-identification-of-mycobacterial-species-using-high-resolution-melting-analysis
#4
Azar D Khosravi, Mohammad Hashemzadeh, Abdolrazagh Hashemi Shahraki, Ali Teimoori
Infections caused by non-tuberculous mycobacteria (NTM) is increasing wordwide. Due to the difference in treatment of NTM infections and tuberculosis, rapid species identification of mycobacterial clinical isolates is necessary for the effective management of mycobacterial diseases treatment and their control strategy. In this study, a cost-effective technique, real-time PCR coupled with high-resolution melting (HRM) analysis, was developed for the differentiation of Mycobacterial species using a novel rpoBC sequence...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29050776/blood-antimicrobial-activity-varies-against-different-mycobacterium-spp
#5
Octavio Miguel Rivero-Lezcano, Sara Blanco-Conde, Ramiro López-Medrano, Eduardo López-Fidalgo, Manuela Caño-Herrero, Teresa Nebreda-Mayoral
In vitro analysis of mycobacterial pathogenicity or host susceptibility has traditionally relied on the infection of macrophages, the target cell of mycobacteria, despite difficulties reproducing their antimycobacterial activity. We have employed alternative models, namely whole blood and leukocytes in plasma, from QuantiFERON negative individuals, and performed infections with the pathogenic M. tuberculosis, the less pathogenic M. avium, M. kansasii and M. chelonae and the occasionally pathogenic M. gordonae and M...
December 2017: Tuberculosis
https://www.readbyqxmd.com/read/29030295/the-new-phylogeny-of-the-genus-mycobacterium-the-old-and-the-news
#6
Enrico Tortoli, Tarcisio Fedrizzi, Conor J Meehan, Alberto Trovato, Antonella Grottola, Elisabetta Giacobazzi, Giulia Fregni Serpini, Sara Tagliazucchi, Anna Fabio, Clotilde Bettua, Roberto Bertorelli, Francesca Frascaro, Veronica De Sanctis, Monica Pecorari, Olivier Jousson, Nicola Segata, Daniela M Cirillo
BACKGROUND: Phylogenetic studies of bacteria have been based so far either on a single gene (usually the 16S rRNA) or on concatenated housekeeping genes. For what concerns the genus Mycobacterium these approaches support the separation of rapidly and slowly growing species and the clustering of most species in well-defined phylogenetic groups. The advent of high-throughput shotgun sequencing leads us to revise conventional taxonomy of mycobacteria on the light of genomic data. For this purpose we investigated 88 newly sequenced species in addition to 60 retrieved from GenBank and used the Average Nucleotide Identity pairwise scores to reconstruct phylogenetic relationships within this genus...
October 11, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28990320/non-tuberculosis-mycobacterium-speciation-using-hplc-under-revised-national-tb-control-programme-rntcp-in-india
#7
George Sebastian, Sharath Burugina Nagaraja, Telagi Vishwanatha, Mamatha Voderhobli, Narayana Vijayalakshmi, Prahlad Kumar
AIMS: Non-Tuberculous Mycobacteria (NTM) are ubiquitous in nature. The data on prevalence of NTM under the RNTCP is scarce. Many NTM species have clinical significance, and hence their identification and speciation are important. METHODS AND RESULT: It is a cross-sectional study conducted at the five RNTCP accredited culture and drug susceptibility testing (CDST) laboratory. The culture isolates from AFB positive but Immunochromatographic test negative samples were taken for identification and speciation using HPLC...
October 9, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28940325/evaluation-of-the-rt-lamp-and-lamp-methods-for-detection-of-mycobacterium-tuberculosis
#8
Dandan Wu, Jiwen Kang, Baosheng Li, Dianxing Sun
BACKGROUND: The current methods for detecting Mycobacterium tuberculosis (Mtb) are not clinically optimal. Standard culture methods (SCMs) are slow, costly, or unreliable, and loop-mediated isothermal amplification (LAMP) cannot differentiate live Mtb. METHODS: This study compared reverse transcription (RT)-LAMP, LAMP, and an SCM for detecting Mtb. A first experiment tested the sensitivity and specificity of primers for 9 species of Mycobacterium (H37Rv, M. intracellulare, M...
September 22, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28932687/multifocal-ulceronecrotic-skin-lesions-a-stigmatizing-case
#9
Fanny-Emmanuelle Bernier, Simon Grandjean Lapierre, Arwa El-Housseini, Mélissa Nantel-Battista, Sapha Barkati
Mycobacterium chelonae is a rapidly growing nontuberculous mycobacteria, ubiquitous in the environment and mostly involved in skin, soft tissue and bone infections. An atypical, severe and multifocal dermatological manifestation of Mycobacterium chelonae infection in an immunocompetent patient is described here. The lesions completely resolved using a multidisciplinary approach. This case emphasizes the important aspects of diagnosis, antimicrobial and surgical management.
2017: IDCases
https://www.readbyqxmd.com/read/28912980/sporotrichoid-like-spread-of-cutaneous-mycobacterium-chelonae-in-an-immunocompromised-patient
#10
Daria Marley Kemp, Anusha G Govind, Jun Kang, Caroline C Brugger, Young C Kauh
Mycobacterium chelonae is a rapidly growing mycobacterium found in water and soil that can cause local cutaneous infections in immunocompetent hosts but more frequently affects immunocompromised patients. Typically, patients will present with painful subcutaneous nodules of the joints or soft tissues from traumatic inoculation. However, exhibiting a sporotrichoid-like pattern of these nodules is uncommon. Herein, we report a case of sporotrichoid-like distribution of cutaneous Mycobacterium chelonae in a patient with systemic lupus erythematosus on significant immunosuppressive medications...
2017: Case Reports in Dermatological Medicine
https://www.readbyqxmd.com/read/28895525/description-of-mycobacterium-chelonae-subsp-bovis-subsp-nov-isolated-from-cattle-bos-taurus-coreanae-emended-description-of-mycobacterium-chelonae-and-creation-of-mycobacterium-chelonae-subsp-chelonae-subsp-nov
#11
Byoung-Jun Kim, Ga-Na Kim, Bo-Ram Kim, Che Ok Jeon, Joseph Jeong, Seon Ho Lee, Ji-Hun Lim, Seung-Heon Lee, Chang Ki Kim, Yoon-Hoh Kook, Bum-Joon Kim
Three rapidly growing mycobacterial strains, QIA-37(T), QIA-40 and QIA-41, were isolated from the lymph nodes of three separate Korean native cattle, Hanwoo (Bos taurus coreanae). These strains were previously shown to be phylogenetically distinct but closely related to Mycobacterium chelonae ATCC 35752(T) by taxonomic approaches targeting three genes (16S rRNA, hsp6 and rpoB) and were further characterized using a polyphasic approach in this study. The 16S rRNA gene sequences of all three strains showed 99...
October 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28879868/mycobacterium-chelonae-infection-complicating-traumatic-and-surgical-wounds-a-case-series
#12
Soumya Jagadeesan, V Anilkumar, Vinitha Varghese Panicker, Gopikrishnan Anjaneyan, Jacob Thomas
Mycobacterium chelonae is a rapidly growing non-tuberculous mycobacterium. The skin and soft tissue infections due to this organism are steadily on the rise and need to be delineated specifically as most of these are not responsive to routine antituberculosis treatment. Here, we report 3 different presentations caused by Mycobacterium chelonae in traumatic and surgical wounds. Mycobacterium chelonae can complicate surgical or traumatic wounds.This infection may also present as injection site abscesses. Diabetics on insulin injections are especially at risk...
September 6, 2017: Indian Journal of Dermatology, Venereology and Leprology
https://www.readbyqxmd.com/read/28857733/mycobacterium-stephanolepidis-sp-nov-a-rapidly-growing-species-related-to-mycobacterium-chelonae-isolated-from-marine-teleost-fish-stephanolepis-cirrhifer
#13
Hanako Fukano, Shinpei Wada, Osamu Kurata, Kinya Katayama, Nagatoshi Fujiwara, Yoshihiko Hoshino
A previously undescribed rapidly growing, non-pigmented mycobacterium was identified based on biochemical and nucleic acid analyses, as well as growth characteristics. Seven isolates were cultured from samples collected from five thread-sail filefish (Stephanolepis cirrhifer) and two farmed black scraper (Thamnaconus modestus). Bacterial growth occurred at 15-35 °C on Middlebrook 7H11 agar. The bacteria were positive for catalase activity at 68 °C and urease activity, intermediate for iron uptake, and negative for Tween 80 hydrolysis, nitrate reduction, semi-quantitative catalase activity and arylsulfatase activity at day 3...
August 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28855835/distribution-of-non-tuberculosis-mycobacteria-strains-from-suspected-tuberculosis-patients-by-heat-shock-protein-65-pcr-rflp
#14
Ali Nour-Neamatollahie, Nayereh Ebrahimzadeh, Seyed Davar Siadat, Farzam Vaziri, Mona Eslami, Abbas Akhavan Sepahi, Sharareh Khanipour, Morteza Masoumi, Fatemeh Sakhaee, Morteza Ghazanfari Jajin, Ahmadreza Bahrmand, Abolfazl Fateh
The genus Mycobacterium contains more than 150 species. Non-tuberculosis mycobacteria (NTM) often cause extrapulmonary and pulmonary disease. Mycobacteria detection at species level is necessary and provides useful information on epidemiology and facilitates successful treatment of patients. This retrospective study aimed to determine the incidence of the NTM isolates and Mycobacterium tuberculosis (Mtb) in clinical specimens collected from Iranian patients during February 2011-December 2013, by PCR-restriction fragment length polymorphism analysis (PRA) of the hsp65 gene...
September 2017: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/28818905/complete-genome-sequence-of-mycobacterium-stephanolepidis
#15
Hanako Fukano, Mitsunori Yoshida, Yukie Katayama, Tsutomu Omatsu, Tetsuya Mizutani, Osamu Kurata, Shinpei Wada, Yoshihiko Hoshino
Mycobacterium stephanolepidis is a rapid-growing nonpigmented species isolated from marine teleost fish (Stephanolepis cirrhifer) and is closely related to Mycobacterium chelonae Here, we report the complete sequence of its genome, comprising a 4.9-Mb chromosome. The sequence represents essential data for future phylogenetic and comparative genome studies of this fish pathogen.
August 17, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28817307/geographic-distribution-of-nontuberculous-mycobacterial-species-identified-among-clinical-isolates-in-the-united-states-2009-2013
#16
Alicen B Spaulding, Yi Ling Lai, Adrian M Zelazny, Kenneth N Olivier, Sameer S Kadri, D Rebecca Prevots, Jennifer Adjemian
RATIONALE: Nontuberculous mycobacteria are an important cause of morbidity in the United States, although patient outcomes vary greatly by species. Currently, nationally representative data on the distribution of mycobacterial species from clinical isolates are limited. OBJECTIVES: Using a national hospitalization database capturing microbiologic data for nearly 6 million patient encounters, we describe the geographic distribution of, and patient demographic features associated with, clinical mycobacterial isolates in the United States...
November 2017: Annals of the American Thoracic Society
https://www.readbyqxmd.com/read/28769591/an-unusual-case-of-spontaneous-mycobacterium-chelonae-corneal-ulcer-in-a-healthy-middle-aged-adult-retraction
#17
(no author information available yet)
[This retracts the article on p. 121 in vol. 9, PMID: 27274315.].
2017: International Medical Case Reports Journal
https://www.readbyqxmd.com/read/28754065/acute-disseminated-mycobacteriosis-in-captive-atlantic-guitarfish-rhinobatos-lentiginosus
#18
Kathryn A Tuxbury, Samuel A Young, Daniel S Bradway, Jamie L Marola, Max Salfinger, Michael M Garner
An adult female captive-born Atlantic guitarfish ( Rhinobatos lentiginosus) was found acutely moribund on exhibit and died soon after presentation. Abnormalities on autopsy were focal cutaneous erythema on the tail, a small liver, many variably sized friable ovarian follicles, and coelomic effusion. Histologic examination revealed systemic bacterial embolization, and yolk coelomitis with minimal associated inflammation and some mineralization. Bacterial culture of blood and coelomic effusion grew a rapidly growing Mycobacterium species that was further identified as Mycobacterium chelonae by PCR amplification and sequencing of the RNA polymerase subunit beta ( rpoB) gene of isolated genomic DNA...
November 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28724491/comparison-of-antemortem-and-environmental-samples-for-zebrafish-health-monitoring-and-quarantine
#19
Marcus J Crim, Christian Lawrence, Robert S Livingston, Andrei Rakitin, Shane J Hurley, Lela K Riley
Molecular diagnostic assays offer both exquisite sensitivity and the ability to test a wide variety of sample types. Various types of environmental sample, such as detritus and concentrated water, might provide a useful adjunct to sentinels in routine zebrafish health monitoring. Similarly, antemortem sampling would be advantageous for expediting zebrafish quarantine, without euthanasia of valuable fish. We evaluated the detection of Mycobacterium chelonae, M. fortuitum, M. peregrinum, Pseudocapillaria tomentosa, and Pseudoloma neurophilia in zebrafish, detritus, pooled feces, and filter membranes after filtration of 1000-, 500-, and 150-mL water samples by real-time PCR analysis...
July 1, 2017: Journal of the American Association for Laboratory Animal Science: JAALAS
https://www.readbyqxmd.com/read/28719321/association-of-interferon-%C3%AE-receptor-1-gene-polymorphism-with-nontuberculous-mycobacterial-lung-infection-among-iranian-patients-with-pulmonary-disease
#20
COMPARATIVE STUDY
Poopak Farnia, Jalaledin Ghanavi, Shima Saif, Parissa Farnia, Ali Akbar Velayati
Nontuberculous mycobacteria (NTM) cause significant pulmonary infections in humans. Researchers have reported an association between interferon-gamma receptor-1 (IFN-γR1 or IFNGR1) deficiency and susceptibility to NTM, but the relevance of polymorphism within these genes is not yet clear. In this study, a single nucleotide polymorphism (SNP), T to C, at position-56 in NTM patients with pulmonary disease was investigated. Molecular identification of Mycobacterium isolates was performed with hsp65 genes using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP)...
July 2017: American Journal of Tropical Medicine and Hygiene
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