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https://www.readbyqxmd.com/read/29027064/light-scattering-measurements-in-electron-beam-sterilized-corneas-stored-in-recombinant-human-serum-albumin
#1
Khoa D Tran, Yan Li, Jeffrey D Holiman, Maolong Tang, David Huang, Michael D Straiko, Christopher G Stoeger
Irradiated corneal tissues have been used for a variety of ophthalmic procedures including glaucoma drainage device covers and lamellar grafts. The maintenance of corneal clarity is important, as light obstructions resulting from processing or long-term storage of irradiated corneas may negatively affect vision and postoperative cosmesis. It has been reported that corneal tissues can be preserved in human serum albumin (HSA), however, the clarity of corneas after long-term storage in HSA has not been well described...
October 12, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29025661/low-systemic-toxicity-nanocarriers-fabricated-from-heparin-mpeg-and-pamam-dendrimers-for-controlled-drug-release
#2
Vu Minh Thanh, Thi Hiep Nguyen, Tuong Vi Tran, Uyen-Thi Phan Ngoc, Minh Nhat Ho, Thi Thinh Nguyen, Yen Nguyen Tram Chau, Van Thu Le, Ngoc Quyen Tran, Cuu Khoa Nguyen, Dai Hai Nguyen
In this report, poly(amide amine) (PAMAM) dendrimer and Heparin-grafted-monomethoxy polyethylene glycol (HEP-mPEG) were synthesized and characterized. In aqueous solution, the generation 4 PAMAM dendrimers (G4.0-PAMAM) existed as nanoparticles with particle size of 5.63nm. However, after electrostatic complexation with HEP-mPEG to form a core@shell structure G4.0-PAMAM@HEP-mPEG, the size of nanoparticles was significantly increased (73.82nm). The G4.0-PAMAM@HEP-mPEG nanoparticles showed their ability to effectively encapsulate doxorubicin (DOX) for prolonged and controlled release...
January 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28970982/concomitant-reverse-hill-sachs-lesion-and-posterior-humeral-avulsion-of-the-glenohumeral-ligament-treatment-with-fresh-talus-osteochondral-allograft-and-arthroscopic-posterior-humeral-avulsion-of-the-glenohumeral-ligament-and-labrum-repair
#3
Justin J Mitchell, Alexander R Vap, George Sanchez, Daniel J Liechti, Jorge Chahla, Gilbert Moatshe, Márcio B Ferrari, Matthew T Provencher
Chronic posterior glenohumeral joint instability can be a challenging clinical entity for patients and surgeons alike. In the setting of a posterior dislocation, a large anterior humeral impaction injury (reverse Hill-Sachs [HS]) may occur, leading to engagement of the humerus with the posterior glenoid bone, especially during internal rotation of the joint. A reverse HS is especially debilitating because of the significant portion of affected humeral head cartilage, and is made worse in the setting of ligamentous disruption such as a posterior humeral avulsion of the glenohumeral ligament (HAGL) lesions...
August 2017: Arthroscopy Techniques
https://www.readbyqxmd.com/read/28958037/evaluation-of-changes-in-cartilage-viability-in-detergent-treated-tracheal-grafts-for-immunosuppressant-free-allotransplantation-in-dogs
#4
Tao Lu, Yiwei Huang, Yulei Qiao, Yongxing Zhang, Yu Liu
OBJECTIVES: The first tissue-engineered clinical tracheal transplant prepared using the detergent-enzymatic method resulted in graft stenosis, possibly from detergent-enzymatic method-induced graft non-viability. We reported on the transplantation of de-epithelialized tracheal allografts while maintaining cartilage viability in dogs. No lethal stenosis occurred in allografts. Herein, on the basis of previous experimentation, we assessed cartilage viability in detergent-treated cartilages...
September 6, 2017: European Journal of Cardio-thoracic Surgery
https://www.readbyqxmd.com/read/28956078/-attitude-towards-organ-and-tissue-donation-in-europe-prerequisite-for-osteochondral-allograft-treatment
#5
REVIEW
S Schmidt, A Schulte, S Schwarz, N Hofmann, S Tietz, M Boergel, S U Sixt
BACKGROUND: The biggest obstacle to overcome for routine treatment of various pathologies with fresh osteochondral allograft is the availability of tissue for transplantation. Large fresh osteochondral allografts are usually harvested from organ donors, but in contrast to organs, tissues can be procured after cardiac arrest. OBJECTIVE: Medical staff as well the general public are much less aware of the possibilities and requirements of tissue donation compared to organ donation...
September 27, 2017: Der Unfallchirurg
https://www.readbyqxmd.com/read/28942372/restoration-of-fresh-cat-ovarian-tissue-function-by-autografting-to-subcutaneous-tissue-a-pilot-study
#6
Ellen C R Leonel, Janice M V Vilela, Raísa E G Paiva, José L P R Jivago, Rodrigo S Amaral, Carolina M Lucci
Ovarian tissue transplantation could be a valuable technique for the preservation of endangered animals. The domestic cat affords an adequate experimental model for studies aimed at wild felids due to its phylogenetic similarity. Thus, this pilot study evaluated the efficacy of cat ovarian tissue autotransplantation to a peripheral site. Three adult queens were submitted to ovariohysterectomy. The ovaries were fragmented into eight pieces; two were fixed as a control and six were transplanted to subcutaneous tissue of the dorsal neck...
September 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28937784/lacertus-fibrosus-versus-achilles-allograft-reconstruction-for-distal-biceps-tears-a-biomechanical-study
#7
Anand M Murthi, Miguel A Ramirez, Brent G Parks, Shannon R Carpenter
BACKGROUND: The bicipital aponeurosis, or lacertus fibrosus, can potentially be used as a reconstruction graft in chronic distal biceps tendon tears. PURPOSE: To evaluate construct stiffness, load to failure, and failure mechanism with lacertus fibrosus versus Achilles allograft for distal biceps tendon reconstruction. STUDY DESIGN: Controlled laboratory study. METHODS: Ten fresh-frozen matched cadaveric pairs of elbows were used...
September 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28925558/endothelial-precursor-cell-cross-match-using-tie-2-enriched-spleen-cells
#8
Volker Daniel, Caner Süsal, Sabine Scherer, Hien Tran, Petra Gombos, Karina Trojan, Mahmoud Sadeghi, Christian Morath, Gerhard Opelz
BACKGROUND: Non-HLA antibodies against human endothelial progenitor cells (EPC) in pre-transplant recipient serum can have a deleterious influence on the graft. EPC enriched from peripheral blood have been commonly used for EPC cross-matching. In the present study, we describe cross-matches using EPC enriched from fresh or frozen-thawed spleen cell preparations, thereby widening the sample source for deceased-donor cross-matching and retrospective studies. METHODS: EPC cross-matches were performed retrospectively using spleen cells and the flow cytometric XM-ONE cross-match test kit...
September 19, 2017: Clinical Transplantation
https://www.readbyqxmd.com/read/28922324/biological-properties-and-therapeutic-value-of-cryopreserved-fat-tissue
#9
Takanobu Mashiko, Szu-Hsien Wu, Koji Kanayama, Rintaro Asahi, Takako Shirado, Masanori Mori, Ataru Sunaga, Shunji Sarukawa, Hirokazu Uda, Kotaro Yoshimura
BACKGROUND: Fat grafting frequently requires multiple treatments and thus repeated liposuction to achieve treatment goals. The purpose of this study is to evaluate whether cryopreservation of adipose tissue may facilitate future fat grafting. METHODS: Lipoaspirates were harvested from six adult females and preserved using two cryopreservation methods: 1) simple cooling to -80˚C (cryo-1); or 2) programmed cooling to -190˚C (cryo-2). Fresh fat, cryo-1 fat, and cryo-2 fat were analyzed both in vitro and in vivo...
September 14, 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28915249/effect-of-treatment-with-angiopoietin-2-and-vascular-endothelial-growth-factor-on-the-quality-of-xenografted-bovine-ovarian-tissue-in-mice
#10
Hyun Sun Kong, Jaewang Lee, Hye Won Youm, Seul Ki Kim, Jung Ryeol Lee, Chang Suk Suh, Seok Hyun Kim
Cryopreservation and transplantation of ovarian tissue (OT) represents a method for fertility preservation. However, as the transplantation is performed without vessel anastomosis, unavoidable ischemic damage occurs. To reduce this ischemic damage and improve outcomes after transplantation, we used two kind of angiogenic factors, angiopoietin-2 (ang-2) and vascular endothelial growth factor (VEGF). Fresh or vitrified-warmed bovine OTs were prepared for xenotransplantation (XT). Fresh OTs were immediately xenografted into nude mice (XT-Fresh)...
2017: PloS One
https://www.readbyqxmd.com/read/28906587/vascularized-thumb-metacarpal-periosteal-pedicled-flap-for-scaphoid-nonunion-an-anatomical-study-and-pediatric-case-report
#11
Sergi Barrera-Ochoa, Gerardo Mendez-Sanchez, Alfonso Rodriguez-Baeza, Jorge Knörr, Jayme Augusto Bertelli, Francisco Soldado
PURPOSE: Through an anatomical review, the primary aim of this study was to delineate the dorsal thumb metacarpal (TM) periosteal branches of the radial artery (RA). In addition, we report here the clinical utility of a vascularized TM periosteal pedicled flap (VTMPF), supplied by the first dorsal metacarpal artery (FDMA), in a complex case of scaphoid nonunion. METHODS: Ten latex-colored upper limbs from fresh human cadavers were used. Branches of the RA were dissected under 3x loupe magnification, noting the periosteal branches arising from the FDMA...
September 14, 2017: Microsurgery
https://www.readbyqxmd.com/read/28905475/evaluation-of-volumetric-dimensional-changes-in-posterior-extraction-sites-with-and-without-arp-using-a-novel-imaging-device
#12
Carolina Sbordone, Paolo Toti, Ranieri Martuscelli, Franco Guidetti, Massimo Porzio, Ludovico Sbordone
BACKGROUND: Alveolar ridge volume loss may be minimized when postextraction sockets are filled by bone substitutes. PURPOSE: The aim of the study was to measure the effect of alveolar ridge preservation (ARP) in maintaining the external contour of the ridge after fresh socket grafting with or without particulate anorganic bovine bone mineral (BBM) and resorbable barrier covering. MATERIALS AND METHODS: In the present controlled study, patients subjected to single-tooth extraction were allocated to 2 groups: postextraction sockets grafted with bovine bone mineral (bbm), and naturally healing sockets (nat)...
September 14, 2017: Clinical Implant Dentistry and related Research
https://www.readbyqxmd.com/read/28880606/fibular-lengthening-osteotomy-to-correct-a-malunited-ankle-fracture-using-fresh-frozen-femoral-head-allograft
#13
Harry J Visser, Khawar Malik, Robert A Djali
Malreduction of a distal fibular fracture can lead to degenerative changes in the ankle joint. Previous studies have shown that the selective use of various fibular reconstructive osteotomies may halt the progression of degenerative arthritis by restoring the normal tibiotalar contact area and decreasing the stresses on the articular cartilage. In this case report, we achieved alignment with restoration of the talocrural angle and Shenton's line of the ankle using a transfibular osteotomy and an allogeneic fresh-frozen femoral head graft to fill the resultant defect...
July 2017: Journal of the American Podiatric Medical Association
https://www.readbyqxmd.com/read/28877604/the-relative-contribution-to-small-finger-abduction-of-the-ulnar-versus-radial-slip-of-the-edm-implications-for-tendon-transfers
#14
Sheriff D Akinleye, Maya Deza Culbertson, Giacomo Cappelleti, Garret Garofolo, Jack Choueka
BACKGROUND: The extensor digiti minimi (EDM) tendon is commonly divided into a radial slip (EDM-R) and an ulnar slip (EDM-U). To our knowledge, the degree to which each EDM slip concomitantly abducts the small finger with active extension has not been formally tested. This study sought to characterize the comparative contributions of finger abduction inherent to each slip of the EDM to observe the sequelae of active small finger extension following transfer of the contralateral slip. METHODS: Eighteen fresh-frozen cadaveric hands were used in this study...
September 1, 2017: Hand: Official Journal of the American Association for Hand Surgery
https://www.readbyqxmd.com/read/28865886/posterior-hypospadias-evaluation-of-a-paradigm-shift-from-single-to-staged-repair
#15
H Badawy, S Orabi, A Hanno, H Abdelhamid
INTRODUCTION: Repair of posterior hypospadias is a current dilemma. Single versus staged repair is the main question to answer. The answer is not easily reached due to lack of comparative studies. Hence such studies are not available, the reports of a change from one approach to the other one are important to point out the results of each procedure in the same hands and in the same center. Herein, we report our results of the repair of posterior hypospadias shifting from single stage to staged repair...
August 9, 2017: Journal of Pediatric Urology
https://www.readbyqxmd.com/read/28865572/torsional-appearance-of-the-anterior-cruciate-ligament-explaining-ribbon-and-double-bundle-concepts-a-cadaver-based-study
#16
Thibaut Noailles, Philippe Boisrenoult, Matthieu Sanchez, Philippe Beaufils, Nicolas Pujol
PURPOSE: To investigate the effect of the anterior cruciate ligament (ACL) torsion in 90° knee flexion on the morphological appearance of the ACL. METHODS: Sixty knees from fresh frozen anatomical specimens were dissected. Eighteen knees were excluded according to selection criteria (torn ACL, mucoid degeneration of the ACL, arthritic lesions of the notch, or knees harboring synovial inflammatory pathologies). After the removal of the synovial membrane, the morphology of the ligamentous fibers of the ACL and the twist were analyzed...
September 2017: Arthroscopy: the Journal of Arthroscopic & related Surgery
https://www.readbyqxmd.com/read/28856732/predictive-model-and-risk-factors-associated-with-a-revised-definition-of-early-allograft-dysfunction-in-liver-transplant-recipients
#17
R Nicolau-Raducu, A J Cohen, A Bokhari, H Bohorquez, D Bruce, I Carmody, E Bugeaud, J Seal, D Sonnier, B Nossaman, G Loss
INTRODUCTION: Early allograft dysfunction (EAD) is a well-defined clinical syndrome that reflects overall graft function within the first week after transplant. The aim of this study was to further refine the definition for EAD. METHOD: In this study, 1124 patients were included for analysis. Logistic regression was performed to identify markers of liver injury associated with 6-month patient and graft failure. RESULTS: Recursive partitioning identified cut-points for ALT/AST >3000/6000IU/dL observed within 1(st) week, with bilirubin ≥10mg/dL and INR ≥1...
August 30, 2017: Clinical Transplantation
https://www.readbyqxmd.com/read/28855822/responses-of-grafted-tomato-solanum-lycopersiocon-l-to-abiotic-stresses-in-saudi-arabia
#18
Abdulaziz Al-Harbi, Ahmad Hejazi, Abdulrasoul Al-Omran
Quantity and quality of irrigation water are considered the most imperative limiting factors for plant production in arid environment. Adoptions of strategies can minimize crop water consumption while nonexistent yield reduction is considered challenge for scholars especially in arid environment. Grafting is regarded as a promising tool to avoid or reduce yield loss caused by abiotic stresses. Tomato (Solanum lycopersium Mill.), commercial cultivar Faridah was grafted on Unifort rootstock and grown under regulated deficit irrigation (RDI) (100%, 80% and 60% ETc), using two types of irrigation water, fresh (EC = 0...
September 2017: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/28846132/age-related-histologic-and-biochemical-changes-in-auricular-and-septal-cartilage
#19
Kiersten L Riedler, Alireza Shokrani, Alexander Markarian, Laurel M Fisher, Jon-Paul Pepper
OBJECTIVES/HYPOTHESIS: To characterize the histologic and biochemical properties of auricular and septal cartilage and analyze age-related changes in middle-aged to older adults. STUDY DESIGN: Cross-sectional study of auricular and septal cartilage from 33 fresh cadavers. METHODS: Auricular and septal cartilage specimens were stained using Safranin O for glycosaminoglycans, Verhoeff's stain for elastin, and Masson's trichrome for collagen...
August 28, 2017: Laryngoscope
https://www.readbyqxmd.com/read/28841652/fresh-frozen-cortical-strut-allograft-in-two-level-anterior-cervical-corpectomy-and-fusion
#20
Kuang-Ting Yeh, Ru-Ping Lee, Ing-Ho Chen, Tzai-Chiu Yu, Kuan-Lin Liu, Cheng-Huan Peng, Jen-Hung Wang, Pau-Yuan Chang, Wen-Tien Wu
Anterior cervical corpectomy and fusion (ACCF) is one of the main surgical strategies for the management of multilevel cervical spondylotic myelopathy (MCSM). High complication rates of graft bone fracture, resorption, displacement, and fusion collapse or pseudarthrosis have been previously reported. The strategies to prevent the aforementioned complications include using fresh frozen cortical strut allograft (FFCSA) to keep most of the original bone quality and using additional anterior plate fixation to improve the fusion stability and union rate...
2017: PloS One
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