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https://www.readbyqxmd.com/read/28076936/histochemical-and-morphological-aspects-of-fresh-frozen-bone-a-preliminary-study
#1
F S De Ponte, G Cutroneo, R Falzea, G Rizzo, L Catalfamo, A Favaloro, G Vermiglio, M Runci, A Centofanti, G Anastasi
Bone graft are used in dentistry for the reconstruction of severely atrophic jaws. Fresh frozen bone has no osteogenic property but it has osteoconductive and osteoinductive properties because its matrix contains growth factors such as vascular endothelial growth factor. The purpose of the present study was to evaluate morphological and protein expression characteristics of fresh frozen bone before graft and after six months of graft in patients who needed maxillary reconstruction. After 6 month of graft we observed the presence of viable bone as evidenced by full osteocyte lacunae and by the presence of RANKR, osteocalcin positive cells and vascular endothelial growth factor...
December 6, 2016: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28060092/functional-reconstruction-of-nonsegmental-mandibular-defect-with-fresh-frozen-bone-graft-and-delayed-implants-placement
#2
Tommaso Bucci, Pier Francesco Nocini
The loss of teeth and the alveolar bone secondary to resection of mandibular tumors may lead to unfavorable esthetic and functional results with a significant impairment of mastication.The authors report a 53-year-old patient with recurrence of ameloblastoma who was submitted to marginal mandibulectomy. Bone defect was immediately reconstructed using fresh frozen bone graft and 9 months after reconstruction 3 dental implants were inserted in the grafted bone. After 6 months, dental rehabilitation was achieved using a fixed implant-supported prosthesis...
January 5, 2017: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/28056513/is-an-anterolateral-ligament-reconstruction-required-in-acl-reconstructed-knees-with-associated-injury-to-the-anterolateral-structures
#3
Frank R Noyes, Lauren E Huser, Darin Jurgensmeier, James Walsh, Martin S Levy
BACKGROUND: The effect of an anterolateral ligament (ALL) reconstruction on rotational knee stability and corresponding anterior cruciate ligament (ACL) graft forces using multiple knee loading conditions including the pivot-shift phenomenon has not been determined. PURPOSE: First, to determine the rotational stability and ACL graft forces provided by an anatomic bone-patellar tendon-bone ACL reconstruction in the ACL-deficient knee alone and with an associated ALL/iliotibial band (ITB) injury...
January 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28043989/impact-of-thoracic-endovascular-aortic-repair-on-radial-strain-in-an-ex-vivo-porcine-model
#4
Foeke J H Nauta, Hector W L de Beaufort, Michele Conti, Stefania Marconi, Arnoud V Kamman, Anna Ferrara, Joost A van Herwaarden, Frans L Moll, Ferdinando Auricchio, Santi Trimarchi
OBJECTIVES: To quantify the impact of thoracic endovascular aortic repair (TEVAR) on radial aortic strain with the aim of elucidating stent-graft-induced stiffening and complications. METHODS: Twenty fresh thoracic porcine aortas were connected to a mock circulatory loop driven by a centrifugal flow pump. A high-definition camera captured diameters at five different pressure levels (100, 120, 140, 160, and 180 mmHg), before and after TEVAR. Three oversizing groups were created: 0-9% (n = 7), 10-19% (n = 6), and 20-29% (n = 6)...
January 2, 2017: European Journal of Cardio-thoracic Surgery
https://www.readbyqxmd.com/read/28042115/reconstruction-of-non-contained-acetabular-defects-with-impaction-grafting-a-reinforcement-mesh-and-a-cemented-polyethylene-acetabular-component
#5
B S Waddell, A Gonzalez Della Valle
This review summarises the technique of impaction grafting with mesh augmentation for the treatment of uncontained acetabular defects in revision hip arthroplasty. The ideal acetabular revision should restore bone stock, use a small socket in the near-anatomic position, and provide durable fixation. Impaction bone grafting, which has been in use for over 40 years, offers the ability to achieve these goals in uncontained defects. The precepts of modern, revision impaction grafting are that the segmental or cavitary defects must be supported with a mesh; the contained cavity is filled with vigorously impacted morselised fresh-frozen allograft; and finally, acrylic cement is used to stabilise the graft and provide rigid, long-lasting fixation of the revised acetabular component...
January 2017: Bone & Joint Journal
https://www.readbyqxmd.com/read/28039493/new-markers-in-atherosclerosis-thrombospondin-2-thbs-2-and-leukocyte-cell-derived-chemotaxin-2-lect-2-an-immunohistochemical-study
#6
Fatma Cavide Sonmez, Pelin Yildiz, Muhammad Salman Akhtar, Cemalettin Aydin, Osman Sonmez, Nuray Ay, Mehmet Akif Vatankulu
BACKGROUND Current research investigating the role of THBS2 and LECT-2 in atherogenesis is very limited. Therefore, we designed this study to demonstrate the role of THBS-2 and LECT-2 in atherosclerosis at the tissue level in fresh specimens. MATERIAL AND METHODS A total of 32 patients who underwent coronary bypass surgery were enrolled. Aortic wall punch biopsies were obtained at the site of proximal aortosaphenous bypass graft anastomosis. A specimen of left internal mammarian artery (LiMA) was taken from the segment just proximal to its anastomosis...
December 31, 2016: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28029798/should-the-ipsilateral-hamstrings-be-used-for-anterior-cruciate-ligament-reconstruction-in-the-case-of-medial-collateral-ligament-insufficiency
#7
Mirco Herbort, Philipp Michel, Michael J Raschke, Nils Vogel, Martin Schulze, Alexander Zoll, Christian Fink, Wolf Petersen, Christoph Domnick
BACKGROUND: Semitendinosus and gracilis muscles are frequently harvested for autologous tendon grafts for cruciate ligament reconstruction. This study investigated the joint-stabilizing effects of these hamstring muscles in cases of insufficiency of the medial collateral ligament (MCL). HYPOTHESES: First, both the semitendinosus and gracilis muscles can actively stabilize the joint against valgus moments in the MCL-deficient knee. Second, the stabilizing influence of these muscles decreases with an increasing knee flexion angle...
December 1, 2016: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28017469/distal-tibia-allograft-glenoid-reconstruction-in-recurrent-anterior-shoulder-instability-clinical-and-radiographic-outcomes
#8
Matthew T Provencher, Rachel M Frank, Petar Golijanin, Daniel Gross, Brian J Cole, Nikhil N Verma, Anthony A Romeo
PURPOSE: To assess the clinical and radiographic outcomes of patients with recurrent anterior shoulder instability treated with fresh distal tibia allograft (DTA) glenoid reconstruction. METHODS: Consecutive patients with a minimum 15% anterior glenoid bone loss associated with recurrent anterior instability who underwent stabilization with DTA glenoid reconstruction were retrospectively reviewed. Patients were evaluated with the American Shoulder and Elbow Society score, Western Ontario shoulder instability index, and single numerical assessment evaluation score at a minimum 2 years after surgery...
December 22, 2016: Arthroscopy: the Journal of Arthroscopic & related Surgery
https://www.readbyqxmd.com/read/27999505/osteochondral-allograft-transplantation-for-femoral-trochlear-dysplasia
#9
Dharmpal V Vansadia, James R Heltsley, Scott Montgomery, Misty Suri, Deryk G Jones
BACKGROUND: The risk factors for patellofemoral joint instability include laxity of medial patellar restraints, abnormal limb geometry, femoral and tibial malrotation, patella alta, and trochlear dysplasia. Femoral trochlear dysplasia is characterized by a hypoplastic or shallow trochlear groove. CASE REPORT: We report the case of a 31-year-old female with trochlear dysplasia and recurrent patella dislocations, laxity of the medial patellofemoral ligament (MPFL), and high-grade chondromalacia of the trochlea and the patella...
2016: Ochsner Journal
https://www.readbyqxmd.com/read/27987079/benchmarking-the-use-of-blood-products-in-cardiac-surgery-to-stimulate-awareness-of-transfusion-behaviour-results-from-a%C3%A2-four-year-longitudinal-study
#10
C Brouwers, B Hooftman, S Vonk, A Vonk, W Stooker, W H Te Gussinklo, R M Wesselink, C Wagner, M C de Bruijne
INTRODUCTION: Cardiac operations account for a large proportion of the blood transfusions given each year, leading to high costs and an increased risk to patient safety. Therefore, it is important to explore initiatives to reduce transfusion rates. This study aims to provide a benchmark for transfusion practice by inter-hospital comparison of transfusion rates, blood product use and costs related to patients undergoing coronary artery bypass grafting (CABG), valve surgery or combined CABG and valve surgery...
December 16, 2016: Netherlands Heart Journal
https://www.readbyqxmd.com/read/27983908/calcium-concentration-in-culture-medium-as-a-nondestructive-and-rapid-marker-of-osteogenesis
#11
Yohei Tanikake, Manabu Akahane, Akira Furukawa, Yasuaki Tohma, Yusuke Inagaki, Tsutomu Kira, Yasuhito Tanaka
Artificial bones made of β-tricalcium phosphate (β-TCP) combined with bone marrowderived stromal cells (BMSCs) are used for effective reconstruction of bone defects caused by genetic defects, traumatic injury, or surgical resection of bone tumors. However, the selection of constructs with high osteogenic potential before implantation is challenging. The purpose of this study was to determine whether the calcium concentration in BMSC culture medium can be used as a nondestructive and simple osteogenic marker for selecting tissueengineered grafts constructed using β-TCP and BMSCs...
November 29, 2016: Cell Transplantation
https://www.readbyqxmd.com/read/27982194/histologic-and-tomographic-findings-of-bone-block-allografts-in-a-4-years-follow-up-a-case-series
#12
Daniel Deluiz, Luciano Santos Oliveira, Paul Fletcher, Fábio Ramôa Pires, Justine Monnerat Tinoco, Eduardo Muniz Barretto Tinoco
The aim of this paper is to report histologic and tomographic findings of fresh frozen bone block allografts bearing dental implants in functional occlusion in a long-term follow-up. Four patients with implants functionally loaded for 4 years on augmented ridges requiring additional mucogingival surgery or implant placement were included in this case series. Cone-beam tomography scans were compared volumetrically between the baseline (first implant placement) and current images. Biopsies of the grafts were retrieved and sent to histological analysis...
October 2016: Brazilian Dental Journal
https://www.readbyqxmd.com/read/27979764/acrylated-chitosan-for-mucoadhesive-drug-delivery-systems
#13
Yulia Shitrit, Havazelet Bianco-Peled
A new mucoadhesive polymer was synthesized by conjugating chitosan to poly(ethylene glycol)diacrylate (PEGDA) via the Michael type reaction. The product was characterized using NMR. Higher PEGDA grafting efficacy was observed with low molecular weight PEGDA (0.7kDa), compared to long 10kDa PEGDA. The acrylation percentage was calculated based on the reaction of ninhydrin with chitosan, and supported the qualitative NMR findings. The adhesive properties were studied by tensile test and rotating system involving detachment of polymer tablets from a fresh intestine sample...
December 12, 2016: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/27973754/transplantation-of-embryonic-spinal-cord-neurons-to-the-injured-distal-nerve-promotes-axonal-regeneration-after-delayed-nerve-repair
#14
Wenming Zhang, Xinyu Fang, Chaofan Zhang, Wen Li, Wai Man Wong, Yejun Xu, Wutian Wu, Jianhua Lin
Peripheral nerve injury (PNI) usually results in poor functional recovery. Nerve repair is the common clinical treatment for PNI but is always obstructed by the chronic degeneration of the distal stump and muscle. Cell transplantation can alleviate the muscle atrophy after PNI, but the subsequent recovery of the locomotive function is seldom described. In this study, we combined cell transplantation and nerve repair to investigate whether the transplantation of embryonic spinal cord cells could benefit the delayed nerve repair...
December 14, 2016: European Journal of Neuroscience
https://www.readbyqxmd.com/read/27965690/root-to-shoot-hormonal-communication-in-contrasting-rootstocks-suggests-an-important-role-for-the-ethylene-precursor-aminocyclopropane-1-carboxylic-acid-in-mediating-plant-growth-under-low-potassium-nutrition-in-tomato
#15
Cristina Martínez-Andújar, Alfonso Albacete, Ascensión Martínez-Pérez, José Manuel Pérez-Pérez, María José Asins, Francisco Pérez-Alfocea
Selection and breeding of rootstocks that can tolerate low K supply may increase crop productivity in low fertility soils and reduce fertilizer application. However, the underlying physiological traits are still largely unknown. In this study, 16 contrasting recombinant inbred lines (RILs) derived from a cross between domestic and wild tomato species (Solanum lycopersicum × Solanum pimpinellifolium) have been used to analyse traits related to the rootstock-mediated induction of low (L, low shoot fresh weight) or high (H, high shoot fresh weight) vigor to a commercial F1 hybrid grown under control (6 mM, c) and low-K (1 mM, k)...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27911030/acellular-endocardium-as-a-novel-biomaterial-for-the-intima-of-tissue-engineered-small-caliber-vascular-grafts
#16
Feng Wang, Xin Guan, TianYi Wu, JianOu Qiao, ZhaoQing Han, JinLong Wu, XiaoWei Yu, QingJun You
We aimed to investigate whether acellular endocardium can be used as a useful biomaterial for the intima of engineered small-caliber vascular grafts. Fresh endocardium was harvested from the swine left atrium and was decellularized by digestion with the decellularization solution of Triton X-100 and SDS containing DNase I and RNase A. Surface morphological characteristics and Young's modulus were evaluated. To analyze the effect of mechanical characteristics on cell adhesion, the decellularized endocardium was stiffened with 2...
December 2016: Artificial Organs
https://www.readbyqxmd.com/read/27907031/in-vitro-pre-clinical-validation-of-suicide-gene-modified-anti-cd33-redirected-chimeric-antigen-receptor-t-cells-for-acute-myeloid-leukemia
#17
Kentaro Minagawa, Muhammad O Jamil, Mustafa Al-Obaidi, Larisa Pereboeva, Donna Salzman, Harry P Erba, Lawrence S Lamb, Ravi Bhatia, Shin Mineishi, Antonio Di Stasi
BACKGROUND: Approximately fifty percent of patients with acute myeloid leukemia can be cured with current therapeutic strategies which include, standard dose chemotherapy for patients at standard risk of relapse as assessed by cytogenetic and molecular analysis, or high-dose chemotherapy with allogeneic hematopoietic stem cell transplant for high-risk patients. Despite allogeneic hematopoietic stem cell transplant about 25% of patients still succumb to disease relapse, therefore, novel strategies are needed to improve the outcome of patients with acute myeloid leukemia...
2016: PloS One
https://www.readbyqxmd.com/read/27904225/the-inferior-medial-genicular-artery-and-its-vascularization-of-the-pes-anserinus-superficialis-a-cadaveric-study
#18
Lena Hirtler, Manuel Ederer, Mike Faber, Patrick Weninger
BACKGROUND: A common method for reconstruction of the anterior cruciate ligament (ACL) is it's replacement by a free avascular graft, using the gracilis and/or semitendinosus tendons. These grafts pass a vulnerable phase in the ligamentization-process during the 1(st) year after reconstruction. The aims of this study were first to evaluate the vascularization of the pes anserinus superficialis (PAS) by the inferior medial genicular artery (IMGA) and second to develop a pedunculated surgical technique for ACL reconstruction, to preserve a maximal amount of natural vascularization of the tendons inserting at the PAS...
November 2016: Indian Journal of Orthopaedics
https://www.readbyqxmd.com/read/27900298/surgical-time-for-graft-preparation-using-different-suture-techniques
#19
Lawrence Camarda, Sebastian Giambartino, Michele Lauria, Michele Saporito, Vito Triolo, Michele D'Arienzo
BACKGROUND: The purpose of the present study was to compare the operative time for graft preparation using different techniques for graft suturing. MATERIAL AND METHODS: Flexor profundus tendons were harvested from fresh pig hind-leg trotters. Three different suture techniques were investigated: the Krackow stitch (K), the Whipstitch (W), and the Modified Finger-Trap suture (MFT). Tendons were sutured starting at 10 mm from the distal free end of the tendon. The suture configurations of the Krackow stitch and Whipstitch were completed with five suture throws...
April 2016: Muscles, Ligaments and Tendons Journal
https://www.readbyqxmd.com/read/27863947/the-effects-of-low-dose-radiotherapy-on-fresh-osteochondral-allografts-an-experimental-study-in-rabbits
#20
Uğur Gönç, Mehmet Çetinkaya, Mesut Atabek
OBJECTIVE: The aim of this study was to investigate the effects of low-dose fractionated radiotherapy on cartilage degeneration after distal femoral fresh massive osteochondral allograft transplantation. METHODS: Twenty-four New Zealand White rabbits were divided into three groups of 8 rabbits each. All rabbits underwent distal femoral medial condyle fresh massive osteochondral allograft transplantation from California rabbits. The group 1 underwent transplantation without any preliminary process...
October 2016: Acta Orthopaedica et Traumatologica Turcica
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