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https://www.readbyqxmd.com/read/28646555/low-thrombogenic-fibrin-heparin-coating-promotes-in-vitro-endothelialization
#1
Ondřej Kaplan, Teresa Hierlemann, Stefanie Krajewski, Julia Kurz, Martina Nevoralová, Milan Houska, Tomáš Riedel, Zuzana Riedelová, Jana Zárubová, Hans P Wendel, Eduard Brynda
Long-term performance of implanted cardiovascular grafts can be ensured if living endothelium overgrows their surface. Surface modifications to implants are therefore being sought that can encourage endothelialization while preventing thrombus formation until the natural endothelium is formed. In the present study, heparin was covalently attached to a fibrin mesh grown from a PVC substrate surface by the catalytic action of surface immobilized thrombin on a fibrinogen solution. The coating prevented platelet activation, thrombin generation and clot formation, and reduced inflammatory reactions when exposed to fresh human whole blood circulating in a Chandler loop model...
June 24, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28642763/rootstock-sub-optimal-temperature-tolerance-determines-transcriptomic-responses-after-long-term-root-cooling-in-rootstocks-and-scions-of-grafted-tomato-plants
#2
Georgia Ntatsi, Dimitrios Savvas, Vassilis Papasotiropoulos, Anastasios Katsileros, Rita M Zrenner, Dirk K Hincha, Ellen Zuther, Dietmar Schwarz
Grafting of elite cultivars onto tolerant rootstocks is an advanced strategy to increase tomato tolerance to sub-optimal temperature. However, a detailed understanding of adaptive mechanisms to sub-optimal temperature in rootstocks and scions of grafting combinations on a physiological and molecular level is lacking. Here, the commercial cultivar Kommeet was grafted either onto 'Moneymaker' (sensitive) or onto the line accession LA 1777 of Solanum habrochaites (tolerant). Grafted plants were grown in NFT-system at either optimal (25°C) or sub-optimal (15°C) temperatures in the root environment with optimal air temperature (25°C) for 22 days...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28640221/use-of-chitosan-pva-hydrogels-with-copper-nanoparticles-to-improve-the-growth-of-grafted-watermelon
#3
Homero González Gómez, Francisca Ramírez Godina, Hortensia Ortega Ortiz, Adalberto Benavides Mendoza, Valentín Robledo Torres, Marcelino Cabrera De la Fuente
Modern agriculture requires alternative practices that improve crop growth without negatively affecting the environment, as resources such as water and arable land grow scarcer while the human population continues to increase. Grafting is a cultivation technique that allows the plant to be more efficient in its utilization of water and nutrients, while nanoscale material engineering provides the opportunity to use much smaller quantities of consumables compared to conventional systems but with similar or superior effects...
June 22, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28634368/preparation-and-properties-of-a-hyperbranch-structured-polyamine-adsorbent-for-carbon-dioxide-capture
#4
Hui He, Yajie Hu, Shuixia Chen, Linzhou Zhuang, Beibei Ma, Qinghua Wu
A fibrous adsorbent with amino-terminated hyperbranch structure (PP-AM-HBP-NH2) was prepared by grafting hyperbranched polyamine (HBP-NH2) onto the acrylamide-modified polypropylene (PP) fibers. The grafting of AM on PP fibers provided the active sites for introducing HBP-NH2 onto the PP fibers. This kind of "grafting to" procedure to synthesize hyperbranch-structured fiber could overcome the disadvantages of stepwise growth procedure, avoiding the complicated synthesis process and the requirement of strict experimental conditions...
June 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28625922/macrophage-infiltration-regulates-the-adipose-ecm-reconstruction-and-the-fibrosis-process-after-fat-grafting
#5
Junrong Cai, Kaiyang Liu, Guanghui Li, Feng Lu
BACKGROUND: Fat grafting experiences a regeneration process from free lipoaspirate to intact adipose tissue. The adipose extracellular matrix (ECM) provides the structure and biochemical support for the surrounding cells, and is regulated by inflammatory cells, like macrophages. It is hypothesized that transferred fat undergoes ECM remodeling after fat grafting and the process can be regulated by macrophage infiltration. METHODS: Lipoaspirate was injected subcutaneously into the back of nude mice...
June 15, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28618872/outcomes-of-patellofemoral-osteochondral-lesions-treated-with-structural-grafts-in-patients-older-than-40-years
#6
Ryan M Degen, Nathan W Coleman, Danielle Tetreault, Brenda Chang, Greg T Mahony, Christopher L Camp, Shawn G Anthony, Riley J Williams
Background Chondral lesions in the patellofemoral compartment represent a difficult entity to treat among active patients, with no clear consensus on the optimal treatment strategy. The purpose of this study was to review the functional outcomes of patients >40 years old with primary patellofemoral osteochondral lesions who underwent a cartilage restoration procedure with a structural graft. Methods Following institutional review board approval, 35 patients >40 years treated for patellofemoral chondral or osteochondral injuries were retrospectively identified...
July 2017: Cartilage
https://www.readbyqxmd.com/read/28615114/experimental-assessment-of-physician-modified-proximal-scalloped-stent-graft-to-extend-proximal-landing-zone-in-the-aortic-arch
#7
E M Faure, I Khantalin, P A Peyron, L Canaud, C Marty-Ané, P Alric
OBJECTIVE/BACKGROUND: The aim of the study was to assess a model of physician modified scalloped stent graft (PMSG) on currently available thoracic aortic devices to extend the proximal landing zone in either zone 2, 1 or 0 of the aortic arch while preserving flow in the supra-aortic vessels on human cadaveric aorta. METHODS: Fresh human aortas were harvested at autopsy from adult subjects. A proximal scallop was made on the stent grafts based on direct measurements on the aortas to extend the proximal landing zone in zone 0 (n = 5), zone 1 (n = 5), and zone 2 (n = 5)...
June 11, 2017: European Journal of Vascular and Endovascular Surgery
https://www.readbyqxmd.com/read/28614276/wrist-arthrodesis-with-femoral-head-structural-allograft-after-failed-total-wrist-arthroplasty
#8
Louis C Grandizio, Steven Maschke
The failed total wrist arthroplasty represents a challenging reconstructive problem for the upper extremity surgeon. Bone loss, poor bone quality, and soft tissue defects all present technical challenges when considering revision procedures. Our purpose is to describe a technique for wrist arthrodesis after failed total wrist arthroplasty utilizing fresh-frozen femoral head allograft and dorsal plate fixation. We describe a simple method of allograft preparation for this procedure, as graft preparation and implantation can be time consuming and technically challenging...
June 13, 2017: Techniques in Hand & Upper Extremity Surgery
https://www.readbyqxmd.com/read/28612204/three-dimensional-isotropic-magnetic-resonance-imaging-can-provide-a-reliable-estimate-of-the-native-anterior-cruciate-ligament-insertion-site-anatomy
#9
Daisuke Araki, Eric Thorhauer, Scott Tashman
PURPOSE: This study quantified the error in anterior cruciate ligament (ACL) insertion site location and area estimated from three-dimensional (3D) isotropic magnetic resonance imaging (MRI) by comparing to native insertion sites determined via 3D laser scanning. METHODS: Isotropic 3D DESS MRI was acquired from twelve fresh-frozen, ACL-intact cadaver knees. ACL insertion sites were manually outlined in each MRI slice, and the resulting contours combined to determine the 3D insertion site shape...
June 13, 2017: Knee Surgery, Sports Traumatology, Arthroscopy: Official Journal of the ESSKA
https://www.readbyqxmd.com/read/28606762/transient-warming-events-occurring-after-freezing-impairs-umbilical-cord-derived-mesenchymal-stromal-cells-functionality
#10
Dominique Chabot, Tony Tremblay, Isabelle Paré, Renée Bazin, Lionel Loubaki
BACKGROUND: Mesenchymal stromal cells (MSCs) have shown promising results for the treatment of refractory acute graft-versus-host disease. While safety of MSC infusion has been demonstrated, the use of cryopreserved MSCs in clinical trials has raised concerns regarding the retention of their functional activity. This has led to the recommendation by experts in the field to use freshly harvested MSCs, even though this approach is much less practical from a logistic point of view. In the present study, we revisited the impact of cryopreservation on MSC functionality and addressed the possibility that warming events on frozen cells rather than cryopreservation per se could impact MSC functionality...
June 9, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28605001/determination-of-structural-femoral-head-allograft-viability-and-integrity-with-a-novel-diagnostic-tool-spect-ct-a-preliminary-study
#11
Kasım Kilicarslan, Nadir Yalcin, Vedat Bicici, Elif Ozdemir, Izzet Bingol, Seyda Turkolmez
OBJECTIVE: To evaluate the viability and integrity of fresh frozen bulk femoral head allografts obtained from the institutional bone bank that were used to reconstruct severe acetabular defects and to validate the SPECT/CT method which gives both anatomical and functional data for this purpose. METHODS: We retrospectively reviewed 9 patients (6 female, 3 male; mean age 63.6 years). Preoperative and postoperative leg lengths, existence of the Trendelenburg sign, range of motion of the hip, visual analogue score (VAS), Harris Hip Score (HHS) and any complication were assesed at each follow-up...
June 2, 2017: Hip International: the Journal of Clinical and Experimental Research on Hip Pathology and Therapy
https://www.readbyqxmd.com/read/28602591/skin-grafts-from-genetically-modified-%C3%AE-1-3-galactosyltransferase-knockout-miniature-swine-a-functional-equivalent-to-allografts
#12
D A Leonard, C Mallard, A Albritton, R Torabi, M Mastroianni, D H Sachs, J M Kurtz, C L Cetrulo
Burn is associated with a considerable burden of morbidity worldwide. Early excision of burned tissue and skin grafting of the resultant wound has been established as a mainstay of modern burn therapy. However, in large burns, donor sites for autologous skin may be limited. Numerous alternatives, from cadaver skin to synthetic substitutes have been described, each with varying benefits and limitations. We previously proposed the use of genetically modified (alpha-1,3-galactosyl transferase knockout, GalT-KO) porcine skin as a viable skin alternative...
June 8, 2017: Burns: Journal of the International Society for Burn Injuries
https://www.readbyqxmd.com/read/28580254/use-of-a-cutting-instrument-for-fresh-osteochondral-distal-tibia-allograft-preparation-treatment-of-glenoid-bone-loss
#13
Marcio B Ferrari, Anthony Sanchez, George Sanchez, Ramesses Akamefula, Bradley M Kruckeberg, Matthew T Provencher
Glenoid bone loss presents a major risk for glenohumeral instability that has been well recognized as a cause of instability recurrence after attempted Bankart repair, and although most surgeons consider the Latarjet procedure as the gold standard, failures can occur with this technique as well and the search for alternative grafts to address glenoid bone loss is a major topic of ongoing research in the field. Of these techniques, the distal tibia allograft (DTA) has been shown to provide an excellent option to restore glenoid biomechanics, due to its congruency with the humeral head, dense bony quality, and the facility of harvest...
April 2017: Arthroscopy Techniques
https://www.readbyqxmd.com/read/28571706/lactated-ringer-based-storage-solutions-are-equally-well-suited-for-the-storage-of-fresh-osteochondral-allografts-as-cell-culture-medium-based-storage-solutions
#14
Afif Harb, Alexander von Horn, Kornelia Gocalek, Luisa Marilena Schäck, Jan Clausen, Christian Krettek, Sandra Noack, Claudia Neunaber
BACKGROUND: Due to the rising interest in Europe to treat large cartilage defects with osteochondrale allografts, research aims to find a suitable solution for long-term storage of osteochondral allografts. This is further encouraged by the fact that legal restrictions currently limit the use of the ingredients from animal or human sources that are being used in other regions of the world (e.g. in the USA). Therefore, the aim of this study was A) to analyze if a Lactated Ringer (LR) based solution is as efficient as a Dulbecco modified Eagle's minimal essential medium (DMEM) in maintaining chondrocyte viability and B) at which storage temperature (4°C vs...
May 25, 2017: Injury
https://www.readbyqxmd.com/read/28567428/acl-roof-impingement-revisited-does-the-independent-femoral-drilling-technique-avoid-roof-impingement-with-anteriorly-placed-tibial-tunnels
#15
John A Tanksley, Brian C Werner, Evan J Conte, David P Lustenberger, M Tyrrell Burrus, Stephen F Brockmeier, F Winston Gwathmey, Mark D Miller
BACKGROUND: Anatomic femoral tunnel placement for single-bundle anterior cruciate ligament (ACL) reconstruction is now well accepted. The ideal location for the tibial tunnel has not been studied extensively, although some biomechanical and clinical studies suggest that placement of the tibial tunnel in the anterior part of the ACL tibial attachment site may be desirable. However, the concern for intercondylar roof impingement has tempered enthusiasm for anterior tibial tunnel placement...
May 2017: Orthopaedic Journal of Sports Medicine
https://www.readbyqxmd.com/read/28567143/a-new-transalveolar-sinus-lift-procedure-for-single-implant-placement-the-ebanist-technique-a-technical-description-and-case-series
#16
Rosario Rizzo, Vittorio Checchi, Federico Marsili, Antonio Zani, Serena Incerti-Parenti, Luigi Checchi
BACKGROUND: Nowadays, there are many techniques to compensate bone atrophies of the posterior maxilla in order to obtain an implant-supported rehabilitation. OBJECTIVE: This case series describes the Ebanist technique: a sinus lift procedure to be used in case of extremely resorbed bone crests (≤3 mm) allowing simultaneous implant placement. METHODS: With a dedicated cylindrical trephine bur, it is possible to harvest a cylinder of bone from a fresh mineralized frozen homologous bone block graft and to simultaneously create a trapdoor on the recipient site...
2017: Open Dentistry Journal
https://www.readbyqxmd.com/read/28559102/biomechanical-characteristics-of-the-anatomic-rectangular-tunnel-anterior-cruciate-ligament-reconstruction-with-a-bone-patellar-tendon-bone-graft
#17
Tatsuo Mae, Konsei Shino, Ryo Iuchi, Kazutaka Kinugasa, Ryohei Uchida, Shigeto Nakagawa, Hideki Yoshikawa, Ken Nakata
PURPOSE: To clarify 1) the force sharing between two portions of BTB graft in anatomic rectangular tunnel (ART) reconstruction and 2) the knee stability in ART technique under anterior tibial load. METHODS: Eleven fresh cadaveric knees were used. First, anterior-posterior (A-P) laxity was measured with Knee Laxity Tester(®) in response to 134 N of A-P tibial load at 20° on the normal knees. Then ART ACL reconstruction was performed with a BTB graft. For graft, the patellar bone plug and tendon portion was longitudinally cut into half as AM and PL portions...
May 27, 2017: Journal of Orthopaedic Science: Official Journal of the Japanese Orthopaedic Association
https://www.readbyqxmd.com/read/28555207/molar-replacement-with-7-mm-wide-diameter-implants-to-place-the-implant-immediately-or-to-wait-4-months-after-socket-preservation-%C3%A2-1-year-after-loading-results-from-a-randomised-controlled-trial
#18
Marco Tallarico, Erta Xhanari, Milena Pisano, Fulvio Gatti, Silvio Mario Meloni
PURPOSE: To test the hypothesis that there is no difference in clinical, radiographic and aesthetic outcomes positioning single post-extractive 7 mm-diameter implants or waiting 4 months after molar extraction and socket preservation procedure. MATERIAL AND METHODS: Patients requiring one implant-supported single restoration to replace a failing tooth in the molar region of both maxilla and mandible were selected. Patients were randomised according to a parallel group design into two arms: implant installation in fresh extraction sockets grafted with cortico-cancellous heterologous bone and porcine derma (group A) or delayed implant installation 4 months after tooth extraction and socket preservation using the same materials (group B)...
2017: European Journal of Oral Implantology
https://www.readbyqxmd.com/read/28551055/fresh-osteochondral-allografts-procurement-and-tissue-donation-in-europe
#19
S Schmidt, A Schulte, S Schwarz, N Hofmann, S Tietz, M Boergel, S U Sixt
Fresh osteochondral allografts are a well-established treatment for large, full-thickness cartilage defects. The clinical outcome for carefully selected patients is very favorable, especially for the young and active and graft survival up to 25 years has been described in the literature. Furthermore, a high patient satisfaction rate has been reported, but the biggest obstacle to overcome is the availability of tissue for transplantation. Large fresh bone allografts for cartilage damage repair only can be harvested from organ donors following organ removal or cadaveric donors, preferably in the setting of an operation room to minimize possible contamination of the tissue...
May 11, 2017: Injury
https://www.readbyqxmd.com/read/28551052/fresh-osteochondral-allotransplants-outcomes-failures-and-future-developments
#20
M Tschon, F Veronesi, S Giannini, M Fini
Osteochondral allografts are used to treat many different conditions as acute traumatic large-sized lesions, degenerative osteoarthritis, osteochondritis dissecans, avascular necrosis or in case of failure of previous procedures particularly in young patients for whom primary prosthesis is not desirable. Fresh allografts present the advantage of having mature viable hyaline cartilage, not causing donor morbidity, allowing the restoration of even large defects in a single surgical session. Conversely, they could account for risks of disease transmission, immunologic reactions, and for limited availability...
May 11, 2017: Injury
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