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https://www.readbyqxmd.com/read/27911030/acellular-endocardium-as-a-novel-biomaterial-for-the-intima-of-tissue-engineered-small-caliber-vascular-grafts
#1
Feng Wang, Xin Guan, TianYi Wu, JianOu Qiao, ZhaoQing Han, JinLong Wu, XiaoWei Yu, QingJun You
We aimed to investigate whether acellular endocardium can be used as a useful biomaterial for the intima of engineered small-caliber vascular grafts. Fresh endocardium was harvested from the swine left atrium and was decellularized by digestion with the decellularization solution of Triton X-100 and SDS containing DNase I and RNase A. Surface morphological characteristics and Young's modulus were evaluated. To analyze the effect of mechanical characteristics on cell adhesion, the decellularized endocardium was stiffened with 2...
December 2016: Artificial Organs
https://www.readbyqxmd.com/read/27907031/in-vitro-pre-clinical-validation-of-suicide-gene-modified-anti-cd33-redirected-chimeric-antigen-receptor-t-cells-for-acute-myeloid-leukemia
#2
Kentaro Minagawa, Muhammad O Jamil, Mustafa Al-Obaidi, Larisa Pereboeva, Donna Salzman, Harry P Erba, Lawrence S Lamb, Ravi Bhatia, Shin Mineishi, Antonio Di Stasi
BACKGROUND: Approximately fifty percent of patients with acute myeloid leukemia can be cured with current therapeutic strategies which include, standard dose chemotherapy for patients at standard risk of relapse as assessed by cytogenetic and molecular analysis, or high-dose chemotherapy with allogeneic hematopoietic stem cell transplant for high-risk patients. Despite allogeneic hematopoietic stem cell transplant about 25% of patients still succumb to disease relapse, therefore, novel strategies are needed to improve the outcome of patients with acute myeloid leukemia...
2016: PloS One
https://www.readbyqxmd.com/read/27904225/the-inferior-medial-genicular-artery-and-its-vascularization-of-the-pes-anserinus-superficialis-a-cadaveric-study
#3
Lena Hirtler, Manuel Ederer, Mike Faber, Patrick Weninger
BACKGROUND: A common method for reconstruction of the anterior cruciate ligament (ACL) is it's replacement by a free avascular graft, using the gracilis and/or semitendinosus tendons. These grafts pass a vulnerable phase in the ligamentization-process during the 1(st) year after reconstruction. The aims of this study were first to evaluate the vascularization of the pes anserinus superficialis (PAS) by the inferior medial genicular artery (IMGA) and second to develop a pedunculated surgical technique for ACL reconstruction, to preserve a maximal amount of natural vascularization of the tendons inserting at the PAS...
November 2016: Indian Journal of Orthopaedics
https://www.readbyqxmd.com/read/27900298/surgical-time-for-graft-preparation-using-different-suture-techniques
#4
Lawrence Camarda, Sebastian Giambartino, Michele Lauria, Michele Saporito, Vito Triolo, Michele D'Arienzo
BACKGROUND: The purpose of the present study was to compare the operative time for graft preparation using different techniques for graft suturing. MATERIAL AND METHODS: Flexor profundus tendons were harvested from fresh pig hind-leg trotters. Three different suture techniques were investigated: the Krackow stitch (K), the Whipstitch (W), and the Modified Finger-Trap suture (MFT). Tendons were sutured starting at 10 mm from the distal free end of the tendon. The suture configurations of the Krackow stitch and Whipstitch were completed with five suture throws...
April 2016: Muscles, Ligaments and Tendons Journal
https://www.readbyqxmd.com/read/27863947/the-effects-of-low-dose-radiotherapy-on-fresh-osteochondral-allografts-an-experimental-study-in-rabbits
#5
Uğur Gönç, Mehmet Çetinkaya, Mesut Atabek
OBJECTIVE: The aim of this study was to investigate the effects of low-dose fractionated radiotherapy on cartilage degeneration after distal femoral fresh massive osteochondral allograft transplantation. METHODS: Twenty-four New Zealand White rabbits were divided into three groups of 8 rabbits each. All rabbits underwent distal femoral medial condyle fresh massive osteochondral allograft transplantation from California rabbits. The group 1 underwent transplantation without any preliminary process...
November 15, 2016: Acta Orthopaedica et Traumatologica Turcica
https://www.readbyqxmd.com/read/27862079/discarded-livers-find-a-new-life-engineered-liver-grafts-using-hepatocytes-recovered-from-marginal-livers
#6
Basak E Uygun, Maria-Louisa Izamis, Maria Jaramillo, Yibin Chen, Gavrielle Price, Sinan Ozer, Martin L Yarmush
Treatment for end-stage liver failure is restricted by the critical shortage of donor organs; about 4000 people die in the USA while waiting for a transplantable organ. This situation has been a major driving force behind the rise of tissue engineering to build artificial tissues/organs. Recent advancements in creating transplantable liver grafts using decellularized liver scaffolds bring the field closer to clinical translation. However, a source of readily available and highly functional adult hepatocytes in adequate numbers for regenerative liver therapies still remains unclear...
November 8, 2016: Artificial Organs
https://www.readbyqxmd.com/read/27861530/a-modified-protocol-with-improved-detection-rate-for-mis-matched-donor-hla-from-low-quantities-of-dna-in-urine-samples-from-kidney-graft-recipients
#7
Janette Kwok, Leo C W Choi, Jenny C Y Ho, Gavin S W Chan, Maggie M Y Mok, Man-Fei Lam, Wai-Leung Chak, Au Cheuk, Ka-Foon Chau, Matthew Tong, Kwok-Wah Chan, Tak-Mao Chan
BACKGROUND: Urine from kidney transplant recipient has proven to be a viable source for donor DNA. However, an optimized protocol would be required to determine mis-matched donor HLA specificities in view of the scarcity of DNA obtained in some cases. METHODS: In this study, fresh early morning urine specimens were obtained from 155 kidney transplant recipients with known donor HLA phenotype. DNA was extracted and typing of HLA-A, B and DRB1 loci by polymerase chain reaction-specific sequence primers was performed using tailor-made condition according to the concentration of extracted DNA...
2016: PloS One
https://www.readbyqxmd.com/read/27859243/effect-of-infusion-of-mabs-to-tnfrsf25-on-graft-rejection-in-allo-immune-mice-receiving-autologous-marrow-transplantation
#8
Reginald M Gorczynski, Hassan Sadozai, Fang Zhu, Ismat Khatri
Significant barriers to transplantation exist for individuals who are pre-sensitized to donor Ag and have high titres of donor-reactive Ab. We report the effect of autologous BMTx after myeloablation in pre-sensitized mice along with the use of mAbs to TNFRSF25, expressed on Tregs. C57BL/6 mice, which had been sensitized earlier with BALB/c skin allografts, received secondary BALB/c grafts after the primary grafts had been rejected. Subsequently, recipient mice underwent myeloablation with cyclophosphamide and busulphan and were injected with T-cell depleted bone marrow from CD45...
November 17, 2016: Immunology
https://www.readbyqxmd.com/read/27855591/effects-of-perioperative-fluid-replacement-therapy-in-lung-transplant-patients
#9
Emil Björkbom, Pekka Hämmäinen, Alexey Schramko
OBJECTIVES: Approximately 10 to 25 lung transplant procedures are performed annually in Finland, and 1-year survival has been 95% over the last 10 years. Our aim was to find associations between perioperative fluid replacement therapies and postoperative patient outcomes, with special emphasis on the use of colloids and blood products. MATERIALS AND METHODS: We retrospectively evaluated data from 100 patients who underwent lung transplant with cardiopulmonary bypass support in Finland from 2007 to 2013...
November 18, 2016: Experimental and Clinical Transplantation
https://www.readbyqxmd.com/read/27843707/fresh-and-frozen-tissue-engineered-three-dimensional-bone-ligament-bone-constructs-for-sheep-anterior-cruciate-ligament-repair-following-a-2-year-implantation
#10
Vasudevan Mahalingam, Edward M Wojtys, Deneen M Wellik, Ellen M Arruda, Lisa M Larkin
Injuries to the anterior cruciate ligament (ACL) often require surgical reconstruction utilizing tendon grafts to restore knee function and stability. Some current graft options for ACL repair are associated with poor long-term outcomes. Our laboratory has fabricated tissue-engineered bone-ligament-bone (BLB) constructs that demonstrate native ligament regeneration and advancement toward native ACL mechanical properties in a sheep ACL reconstruction model. Prior work has shown that freezing BLBs as a method of preservation resulted in similar outcomes compared with fresh BLBs after 6-month implantation...
2016: BioResearch Open Access
https://www.readbyqxmd.com/read/27833116/freeze-dried-platelet-rich-plasma-accelerates-bone-union-with-adequate-rigidity-in-posterolateral-lumbar-fusion-surgery-model-in-rats
#11
Yasuhiro Shiga, Sumihisa Orita, Go Kubota, Hiroto Kamoda, Masaomi Yamashita, Yusuke Matsuura, Kazuyo Yamauchi, Yawara Eguchi, Miyako Suzuki, Kazuhide Inage, Takeshi Sainoh, Jun Sato, Kazuki Fujimoto, Koki Abe, Hirohito Kanamoto, Masahiro Inoue, Hideyuki Kinoshita, Yasuchika Aoki, Tomoaki Toyone, Takeo Furuya, Masao Koda, Kazuhisa Takahashi, Seiji Ohtori
Fresh platelet-rich plasma (PRP) accelerates bone union in rat model. However, fresh PRP has a short half-life. We suggested freeze-dried PRP (FD-PRP) prepared in advance and investigated its efficacy in vivo. Spinal posterolateral fusion was performed on 8-week-old male Sprague-Dawley rats divided into six groups based on the graft materials (n = 10 per group): sham control, artificial bone (A hydroxyapatite-collagen composite) -alone, autologous bone, artificial bone + fresh-PRP, artificial bone + FD-PRP preserved 8 weeks, and artificial bone + human recombinant bone morphogenetic protein 2 (BMP) as a positive control...
November 11, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27822894/do-needleless-knots-have-similar-strength-as-the-krackow-suture-an-in-vitro-porcine-tendon-study
#12
Chih-Kai Hong, Ting-Hsuan Kuo, Ming-Long Yeh, I-Ming Jou, Cheng-Li Lin, Wei-Ren Su
BACKGROUND: Numerous needleless techniques for tendon graft fixation that feature several advantages have been reported. However, there are few studies that have compared the holding strength between the needleless techniques (rolling hitch and modified rolling hitch) and traditional suture methods. QUESTIONS/PURPOSES: To compare the tendon graft-holding strength of the rolling hitch and modified rolling hitch with the Krackow stitch in an in vitro porcine biomechanical model...
November 7, 2016: Clinical Orthopaedics and related Research
https://www.readbyqxmd.com/read/27822697/annular-ligament-reconstruction-with-the-superficial-head-of-the-brachialis-surgical-technique-and-biomechanical-evaluation
#13
Michael Hackl, Kilian Wegmann, Christian Ries, Sebastian Lappen, Martin Scaal, Lars Peter Müller
PURPOSE: The purpose of this study was to perform biomechanical testing of annular ligament (AL) reconstruction using the superficial head of the brachialis tendon (SHBT) as a distally based tendon graft. We hypothesized that posterior translation of the radial head following AL reconstruction with an SHBT graft does not significantly differ from intact specimens. METHODS: Six fresh-frozen elbow specimens were used. The stability of the radial head against posterior translation forces (30 N) was evaluated in 0°, 45°, 90° and 120° of elbow flexion...
November 7, 2016: Surgical and Radiologic Anatomy: SRA
https://www.readbyqxmd.com/read/27817994/favorable-early-results-of-impaction-bone-grafting-with-reinforcement-mesh-for-the-treatment-of-paprosky-3b-acetabular-defects
#14
Bradford S Waddell, Friedrich Boettner, Alejandro Gonzalez Della Valle
BACKGROUND: We present the early institutional experience with the use of impaction bone grafting, mesh augmentation, and cement fixation of an all-polyethylene cup for the treatment of Paprosky 3B acetabular defects during revision total hip arthroplasty. METHODS: Between 2005 and 2014, 21 patients (9 men, 12 women) with Paprosky 3B acetabular defects who underwent revision total hip arthroplasty using this technique were reviewed clinically and radiographically...
October 5, 2016: Journal of Arthroplasty
https://www.readbyqxmd.com/read/27800212/benefits-of-mineralized-bone-cortical-allograft-for-immediate-implant-placement-in-extraction-sites-an-in-vivo-study-in-dogs
#15
Valérie Orti, Philippe Bousquet, Paul Tramini, Cesar Gaitan, Brenda Mertens, Frédéric Cuisinier
PURPOSE: The aim of the present study was to evaluate the effectiveness of using a mineralized bone cortical allograft (MBCA), with or without a resorbable collagenous membrane derived from bovine pericardium, on alveolar bone remodeling after immediate implant placement in a dog model. METHODS: Six mongrel dogs were included. The test and control sites were randomly selected. Four biradicular premolars were extracted from the mandible. In control sites, implants without an allograft or membrane were placed immediately in the fresh extraction sockets...
October 2016: Journal of Periodontal & Implant Science
https://www.readbyqxmd.com/read/27793804/osteochondral-allograft-donor-host-matching-by-the-femoral-condyle-radius-of-curvature
#16
Derek T Bernstein, Craig A O'Neill, Ryan S Kim, Hugh L Jones, Philip C Noble, Joshua D Harris, Patrick C McCulloch
BACKGROUND: Conventional osteochondral allograft (OCA) matching, requiring orthotopic, size-matched condyles, and narrow surgical time windows often prohibit timely transplantation. HYPOTHESIS: The femoral condyle radius of curvature (RoC) is an appropriate, isolated criterion for donor-host matching in fresh OCAs, potentially enhancing matching efficiency when compared with conventional matching techniques. STUDY DESIGN: Descriptive laboratory study...
October 28, 2016: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/27783263/hla-typing-analysis-following-allogeneic-bone-grafting-for-sinus-lifting
#17
Marcelo Piaia, Carolina Bonet Bub, Guilherme de Menezes Succi, Margareth Torres, Thiago Henrique Costa, Fabricio Costa Pinheiro, Marcelo Henrique Napimoga
According to the Brazilian Association of Organ Transplants, in 2015, 19,408 bone transplants were performed in Brazil, over 90% by Dental Surgeons. The surgical technique itself has a respectable number of reports regarding its clinical efficacy, as measured by long-term survival of dental implants in grafted areas. Uncertainty remains, however, as to whether fresh frozen grafts from human bone donors remain immunologically innocuous in the body of the host. Six male with no previous medical history of note, including systemic diseases, surgery or blood transfusion were selected...
October 25, 2016: Cell and Tissue Banking
https://www.readbyqxmd.com/read/27777334/secondary-merkel-cell-carcinoma-arising-from-a-graft-donor-site
#18
Fatima Aloraifi, Stephen Mulgrew, Nick K James
Merkel cell carcinoma (MCC) is a highly aggressive cutaneous neuroendocrine tumour that is increasing in incidence. We report a case of a 92-year-old white man on long-term immunosuppression for temporal arteritis who presented with a Merkel cell tumour on his left cheek. A wide local excision was performed and the defect was reconstructed with a full-thickness skin graft. Four years later, the patient re-presented with a Merkel cell tumour arising from the right supraclavicular donor site. To our knowledge, this is the first report of a recurrence of MCC into a donor site...
October 24, 2016: Journal of Cutaneous Medicine and Surgery
https://www.readbyqxmd.com/read/27771937/lumbar-intervertebral-disc-allograft-transplantation-healing-and-remodelling-of-the-bony-structure
#19
Y-C Huang, J Xiao, W Y Leung, W W Lu, Y Hu, K D Luk
Previous human study suggested that fresh-frozen intervertebral disc allograft transplantation can relieve neurological symptoms and restore segmental kinematics. Before wide clinical application, research into the pathophysiology of the postoperative disc allograft is needed. One important question that remains to be answered in disc allografting is the healing process of the host-graft interface and the subsequent change of the endplates. With the goat model for lumbar disc allografting, histology, micro-computed tomography analysis, scanning electron microscopy and energy-dispersive X-ray spectroscopy mapping were applied to evaluate the healing of the host-graft interfaces, the remodelling of subchondral bone, and the changes of the bony and cartilaginous endplates after transplantation...
October 19, 2016: European Cells & Materials
https://www.readbyqxmd.com/read/27771585/efficacy-of-freeze-dried-platelet-rich-plasma-in-bone-engineering
#20
Yuya Nakatani, Hideki Agata, Yoshinori Sumita, Takamitsu Koga, Izumi Asahina
OBJECTIVE: Platelet-rich plasma (PRP) is typically isolated and applied immediately after preparation, making it both a time- and labor-intensive addition to the operative procedure. Thus, it would be convenient if PRP could be preserved. We evaluated the efficacy of freeze-dried PRP (FD-PRP), as compared with freshly isolated PRP (f-PRP) for bone engineering. DESIGN: FD-PRP was prepared by lyophilization of f-PRP and was subsequently preserved at -20°C for one month...
January 2017: Archives of Oral Biology
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