keyword
MENU ▼
Read by QxMD icon Read
search

Plant tissue culture, micropropagation

keyword
https://www.readbyqxmd.com/read/28968406/novel-potato-plants-with-enhanced-cadmium-resistance-and-antioxidative-defence-generated-after-in-vitro-cell-line-selection
#1
Seyedardalan Ashrafzadeh, David W M Leung
It is of interest to apply plant tissue culture to generate plants resistant to toxic effects of cadmium (Cd) on plant growth. Callus cultures were initiated from leaf explants of micropropagated potato plantlets (Solanum tuberosum L., cv. Iwa) for in vitro selection comprising 18 different Cd treatments varying in Cd exposure timing and duration. Plantlets regenerated from two different lines of Cd-selected calli, L9 and L11, were found to exhibit enhanced resistance to 218 μM Cd compared to control (source plantlets for leaf explants used to initiate callus cultures for Cd resistance)...
2017: PloS One
https://www.readbyqxmd.com/read/28867986/ecdysteroids-production-in-plant-in-vitro-cultures
#2
REVIEW
Barbara Thiem, Małgorzata Kikowska, Michał P Maliński, Dariusz Kruszka, Marta Napierała, Ewa Florek
Ecdysteroids are secondary metabolites, widely distributed in the animal and plant kingdoms. They have a wide range of pharmacological effects in vertebrates, including mammals, most of which are beneficial for humans. Therefore, they have become compounds of interest for the pharmaceutical industry due to their adaptogenic, anabolic, hypoglycaemic, hypocholesterolaemic and antimicrobial activities, which are still being researched. Nowadays, ecdysteroids are present as active ingredients in bodybuilding supplements...
2017: Phytochemistry Reviews: Proceedings of the Phytochemical Society of Europe
https://www.readbyqxmd.com/read/28755338/indirect-somatic-embryogenesis-from-mature-inflorescence-explants-of-date-palm
#3
Ali M Al-Ali, Chien-Ying Ko, Sultan A Al-Sulaiman, Sami O Al-Otaibi, Abd Ulmoneem H Al-Khamees, Megahed H Ammar
Due to the limitations associated with shoot tip explants in the micropropagation of date palm, inflorescence explants are an ideal alternative. This chapter focuses on the protocol for the induction of callus from inflorescence tissue, establishment for proliferation of somatic embryos, germination, elongation, rooting, and acclimatization. Female inflorescences, 30-40 cm in length, cv. Shaishee, were used for culture initiation. After disinfection, the outer inflorescence cover (spathe) is cut open, and the spikelet explants, 1 cm long, are cultured on modified Murashige and Skoog (MS) medium containing 100 mg/L 2,4-D, 3 mg/L kinetin, and 3 mg/L 2ip and incubated at 25 ± 2 °C in the dark...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28755336/enhanced-indirect-somatic-embryogenesis-of-date-palm-using-low-levels-of-seawater
#4
Rania A Taha
Date palm tolerates salinity, drought, and high temperatures. Arid and semiarid zones, especially the Middle East region, need a huge number of date palms for cultivation. To meet this demand, tissue culture techniques have great potential for mass production of plantlets, especially using the indirect embryogenesis technique; any improvement of these techniques is a worthy objective. Low levels of salinity can enhance growth and development of tolerant plants. A low level of seawater, a natural source of salinity, reduces the time required for micropropagation processes of date palm cv...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28755216/evaluation-of-clonal-fidelity-of-micropropagated-date-palm-by-random-amplified-polymorphic-dna-rapd
#5
Arpan Modi, Bhavesh Gajera, Naraynan Subhash, Nitish Kumar
Date palm is a fruit-bearing tree commonly found in arid and semiarid regions. It is a dioecious plant, producing fruit on female plants and a limited number of basal offshoots for propagation. To produce large numbers of uniform plantlets, tissue culture techniques are required. It is highly advisable to detect genetic variation that may occur through micropropagation techniques as it may lead to phenotypic alterations. Random amplified polymorphic DNA (RAPD) is a simple and PCR-based molecular marker technique which can be employed to check the somaclonal variation...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28755212/in-vitro-cryopreservation-of-date-palm-caulogenic-meristems
#6
Lotfi Fki, Olfa Chkir, Walid Kriaa, Ameni Nasri, Emna Baklouti, Raja B Masmoudi, Alain Rival, Noureddine Drira, Bart Panis
Cryopreservation is the technology of choice not only for plant genetic resource preservation but also for virus eradication and for the efficient management of large-scale micropropagation. In this chapter, we describe three cryopreservation protocols (standard vitrification, droplet vitrification, and encapsulation vitrification) for date palm highly proliferating meristems that are initiated from vitro-cultures using plant growth regulator-free MS medium. The positive impact of sucrose preculture and cold hardening treatments on survival rates is significant...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28743038/physiological-vascular-and-nanomechanical-assessment-of-hybrid-poplar-leaf-traits-in-micropropagated-plants-and-plants-propagated-from-root-cuttings-a-contribution-to-breeding-programs
#7
Jaroslav Ďurkovič, Hana Husárová, Lucia Javoříková, Ingrid Čaňová, Miriama Šuleková, Monika Kardošová, Ivan Lukáčik, Miroslava Mamoňová, Rastislav Lagaňa
Micropropagated plants experience significant stress from rapid water loss when they are transferred from an in vitro culture to either greenhouse or field conditions. This is caused both by inefficient stomatal control of transpiration and the change to a higher light intensity and lower humidity. Understanding the physiological, vascular and biomechanical processes that allow micropropagated plants to modify their phenotype in response to environmental conditions can help to improve both field performance and plant survival...
July 13, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28685479/neoteric-trends-in-tissue-culture-mediated-biotechnology-of-indian-ipecac-tylophora-indica-burm-f-merrill
#8
REVIEW
Saikat Gantait, Suprabuddha Kundu
Tylophora indica (Burm. f.) Merrill, an ethno-pharmacologically important perennial climber of Asclepiadaceae, is commonly known as Antamul or Indian ipecac. It is essentially accredited for its medicinal properties owing to its wide range of alkaloids in the form of bioactive secondary metabolites, such as tylophorine, tylophorinine, and tylophorinidine. Accelerated mass propagation of Tylophora is challenging because of its reduced seed germination frequency that consequently headed the pursuit for efficient protocols on in vitro propagation for the large-scale regeneration, conservation as well as sustainable supply of quality propagules...
July 2017: 3 Biotech
https://www.readbyqxmd.com/read/28526530/insights-into-the-early-stage-of-pinus-nigra-arn-somatic-embryogenesis-using-discovery-proteomics
#9
Katarína Klubicová, Lubica Uvácková, Maksym Danchenko, Peter Nemecek, Ludovít Skultéty, Ján Salaj, Terézia Salaj
The somatic embryogenesis in conifers represents a suitable model of plant regeneration system facilitating studies of fundamental aspects of an early development as well as in vitro micropropagation. The aim of our study was to deeper understand the somatic embryogenesis in the conifer tree Pinus nigra Arn. Comparative proteomic analysis based on 2D-PAGE in 1) proliferating embryogenic tissues (E) initiated from immature zygotic embryos, 2) non-embryogenic calli (NEC) initiated from cotyledons of somatic seedlings of the same genotypes, 3) embryogenic tissues that lost the maturation capacity (E-L) of two cell lines (E362, E366)...
May 16, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28393691/biotechnological-approaches-for-production-of-anti-cancerous-compounds-resveratrol-podophyllotoxin-and-zerumbone
#10
Krishnadas Nandagopal, Mihir Halder, Biswabhusan Dash, Sanghamitra Nayak, Sumita Jha
Secondary metabolites from numerous plant sources have been developed as anti-cancer reagents and compounds such as resveratrol, podophyllotoxin and zerumbone are of particular importance in this regard. Since their de novo chemical synthesis is both arduous and commercially expensive, there has been an impetus to develop viable, biotechnological methods of production. Accordingly, this review focuses on recent developments in the field, highlighting the use of micropropagation, cell suspension cultures, callus cultures, hairy root cultures, recombinant microbes and genetically modified higher plants...
April 4, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28330124/somaclonal-variations-and-their-applications-in-horticultural-crops-improvement
#11
REVIEW
Hare Krishna, Mahdi Alizadeh, Dhurendra Singh, Udayvir Singh, Nitesh Chauhan, Maliheh Eftekhari, Radha Kishan Sadh
The advancements made in tissue culture techniques has made it possible to regenerate various horticultural species in vitro as micropropagation protocols for commercial scale multiplication are available for a wide range of crops. Clonal propagation and preservation of elite genotypes, selected for their superior characteristics, require high degree of genetic uniformity amongst the regenerated plants. However, plant tissue culture may generate genetic variability, i.e., somaclonal variations as a result of gene mutation or changes in epigenetic marks...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28326314/case-study-of-somaclonal-variation-in-resistance-genes-mlo-and-pme3-in-flaxseed-linum-usitatissimum-l-induced-by-nanoparticles
#12
Inese Kokina, Ilona Mickeviča, Marija Jermaļonoka, Linda Bankovska, Vjačeslavs Gerbreders, Andrejs Ogurcovs, Inese Jahundoviča
Nanoparticles influence on genome is investigated worldwide. The appearance of somaclonal variation is a cause of great concern for any micropropagation system. Somaclonal variation describes the tissue-culture-induced phenotypic and genotypic variations. This paper shows the results of somaclonal variation in two resistance genes pectin methylesterase and Mlo-like protein in all tissue culture development stages, as donor plant, calluses, and regenerants of Linum usitatissimum induced by gold and silver nanoparticles...
2017: International Journal of Genomics
https://www.readbyqxmd.com/read/27964780/reversion-of-hyperhydricity-in-pink-dianthus-chinensis-l-plantlets-by-agno3-and-its-associated-mechanism-during-in-vitro-culture
#13
Hongyang Gao, Xiuying Xia, Lijia An, Xin Xin, Yuan Liang
Hyperhydricity occurs frequently in plant tissue culture and can severely affect commercial micropropagation and genetic improvement of the cultured plantlets. Hyperhydric shoots are charaterzized by high water content, but how this occurs is still a subject of investigation. Silver ion (Ag(+)) can reduce the extent of hyperhydricity in plants, but its effect on the reversion of hyperhydric plantlets and the underlying mechanism of reversion has not been clarified. In this study, about 67% of the hyperhydric Dianthus chinensis L...
January 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/27807436/artificial-neural-network-genetic-algorithm-as-powerful-tool-to-predict-and-optimize-in-vitro-proliferation-mineral-medium-for-g-%C3%A3-n15-rootstock
#14
Mohammad M Arab, Abbas Yadollahi, Abdolali Shojaeiyan, Hamed Ahmadi
One of the major obstacles to the micropropagation of Prunus rootstocks has, up until now, been the lack of a suitable tissue culture medium. Therefore, reformulation of culture media or modification of the mineral content might be a breakthrough to improve in vitro multiplication of G × N15 (garnem). We found artificial neural network in combination of genetic algorithm (ANN-GA) as a very precise and powerful modeling system for optimizing the culture medium, So that modeling the effects of MS mineral salts ([Formula: see text], [Formula: see text], [Formula: see text], Ca(2+), K(+), [Formula: see text], Mg(2+), and Cl(-)) on in vitro multiplication parameters (the number of microshoots per explant, average length of microshoots, weight of calluses derived from the base of stem explants, and quality index of plantlets) of G × N15...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27553812/micropropagation-and-genetic-transformation-of-tylophora-indica-burm-f-merr-a-review
#15
REVIEW
Jaime A Teixeira da Silva, Sumita Jha
This review provides an in-depth and comprehensive overview of the in vitro culture of Tylophora species, which have medicinal properties. Tylophora indica (Burm. f.) Merr. is a climbing perennial vine with medicinal properties. The tissue culture and genetic transformation of T. indica, which has been extensively studied, is reviewed. Micropropagation using nodal explants has been reported in 25 % of all publications. Leaf explants from field-grown plants has been the explant of choice of independent research groups, which reported direct and callus-mediated organogenesis as well as callus-mediated somatic embryogenesis...
November 2016: Plant Cell Reports
https://www.readbyqxmd.com/read/27478496/conservation-and-sustainable-use-of-medicinal-plants-problems-progress-and-prospects
#16
Shi-Lin Chen, Hua Yu, Hong-Mei Luo, Qiong Wu, Chun-Fang Li, André Steinmetz
Medicinal plants are globally valuable sources of herbal products, and they are disappearing at a high speed. This article reviews global trends, developments and prospects for the strategies and methodologies concerning the conservation and sustainable use of medicinal plant resources to provide a reliable reference for the conservation and sustainable use of medicinal plants. We emphasized that both conservation strategies (e.g. in situ and ex situ conservation and cultivation practices) and resource management (e...
2016: Chinese Medicine
https://www.readbyqxmd.com/read/27356371/-study-on-rapid-micropropagation-in-vitro-technique-of-guangfeng-medicinal-yam-dioscorea-opposita-plantlets
#17
Ming-Hua Yin, Zhi-Jian Xu, Sheng-qin Zhang, Si-Jie Lv, Yan-hong Zeng, You-zhang Fu, Sen-rong Hong
OBJECTIVE: In order to provide methodology reference for virus-free and germplasm conservation of Guangfeng medicinal yam (Dioscorea opposita) plantlets, rapid micropropagation in vitro technique of Guangfeng medicinal yam plantlets was studied. METHODS: Using the method of plant tissue culture, single factor test and flow-cytometry, the basic procedure of Guangfeng medicinal yam tissue culture was established and the DNA content of Guangfeng medicinal yam plantlets and its potted seedlings was detected...
November 2015: Zhong Yao Cai, Zhongyaocai, Journal of Chinese Medicinal Materials
https://www.readbyqxmd.com/read/27318708/detection-of-variation-in-long-term-micropropagated-mature-pistachio-via-dna-based-molecular-markers
#18
Hülya Akdemir, Veysel Suzerer, Engin Tilkat, Ahmet Onay, Yelda Ozden Çiftçi
Determination of genetic stability of in vitro-grown plantlets is needed for safe and large-scale production of mature trees. In this study, genetic variation of long-term micropropagated mature pistachio developed through direct shoot bud regeneration using apical buds (protocol A) and in vitro-derived leaves (protocol B) was assessed via DNA-based molecular markers. Randomly amplified polymorphic DNA (RAPD), inter-simple sequence repeat (ISSR), and amplified fragment length polymorphism (AFLP) were employed, and the obtained PIC values from RAPD (0...
December 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27246994/effect-of-zinc-oxide-zno-nanoparticles-on-physiology-and-steviol-glycosides-production-in-micropropagated-shoots-of-stevia-rebaudiana-bertoni
#19
Rabia Javed, Muhammad Usman, Buhara Yücesan, Muhammad Zia, Ekrem Gürel
This study aims to address the effects of different concentrations (0, 0.1, 1.0, 10, 100 or 1000 mg L(-1)) of engineered zinc oxide (ZnO) nanoparticles (34 nm in size) on growth parameters, steviol glycosides (rebaudioside A and stevioside) production and antioxidant activities in the tissue culture grown shoots of Stevia rebaudiana Bertoni. The highest percentage of shoot formation (89.6%) at 1 mg L(-1) of ZnO nanoparticles concentration suggests a positive influence of ZnO nanoparticles on S. rebaudiana growth as compared to other treatments with or without ZnO nanoparticles...
January 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/27242948/micropropagation-micromorphological-studies-and-in-vitro-flowering-in-rungia-pectinata-l
#20
Mahipal S Shekhawat, M Manokari, C P Ravindran
A tissue culture protocol was developed for an important medicinal plant Rungia pectinata L. in the present study. Nodal shoots were used as explants and surface-sterilized with 0.1% HgCl2 solution. Murashige and Skoog (MS) medium was used to establish the cultures of R. pectinata. The bud break was reported on MS medium supplemented with 1.0 mg L(-1) 6-benzylaminopurine (BAP). About 98% response was observed with this media combination and maximum 3.2 shoots per explant with 4.3 cm length were recorded...
2016: Scientifica
keyword
keyword
21496
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"