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https://www.readbyqxmd.com/read/28332156/the-pathology-of-failed-mckee-farrar-implants-correlation-with-modern-metal-on-metal-implant-failure
#1
Mitsuru Munemoto, George Grammatopoulos, Yasuhito Tanaka, Max Gibbons, Nicholas A Athanasou
The McKee-Farrar (MF) prosthesis was the first widely used total hip replacement (THR) to employ a metal-on-metal (MoM) articulation. These implants had a high rate of early aseptic loosening but a number achieved good long-term implant survival, stimulating the reintroduction of second and third generation implants of this type. In this study we analysed archival histopathology of periprosthetic tissues in twenty cases of MF aseptic implant failure to determine if there was evidence of an innate and adaptive immune response similar to that seen in modern MoM implants...
May 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28330760/ck2-dependent-phosphorylation-positively-regulates-stress-induced-activation-of-msn2-in-saccharomyces-cerevisiae
#2
Bo-Ram Cho, Ji-Sook Hahn
CK2 is a highly conserved Ser/Thr protein kinase involved in a large number of cellular processes. Here, we demonstrate that CK2-dependent phosphorylation positively regulates Msn2/4, the general stress response transcriptional activators in Saccharomyces cerevisiae, in response to various types of environmental stress conditions. CK2 overexpression elicits hyperactivation of Msn2/4, whereas deletion of one of the CK2 catalytic subunits, especially CKA2, leads to reduced transcriptional activity of Msn2/4 in response to glucose starvation, H2O2, and lactic acid...
March 18, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28329352/costs-and-consequences-of-early-hospital-discharge-after-major-inpatient-surgery-in-older-adults
#3
Scott E Regenbogen, Anne H Cain-Nielsen, Edward C Norton, Lena M Chen, John D Birkmeyer, Jonathan S Skinner
Importance: As prospective payment transitions to bundled reimbursement, many US hospitals are implementing protocols to shorten hospitalization after major surgery. These efforts could have unintended consequences and increase overall surgical episode spending if they induce more frequent postdischarge care use or readmissions. Objective: To evaluate the association between early postoperative discharge practices and overall surgical episode spending and expenditures for postdischarge care use and readmissions...
March 22, 2017: JAMA Surgery
https://www.readbyqxmd.com/read/28323037/a-phosphorylation-wide-sncrna-screen-reveals-protein-functional-effector-sncrnas-pfernas-in-human-lung-somatic-cells
#4
Tyler Gable, Yuyan Wang, David Clark, Priti Kumari, Amol Carl Shetty, Li Mao, Yuping Mei
We recently reported that PIWI-interacting RNAs likes (piR-Ls) could regulate functions of the interacting phosphorylated proteins (p-Proteins). In addition, except for writers and erasers, functional efficacy of p-Proteins on their readers still remains unknown. We, therefore, reasoned there was a type of sncRNAs which could regulate functional efficacy of p-Proteins. Here, we profiled sncRNAs interacting with phosphorylated -Ser, -Thr and -Tyr residues in 3 HBE and 4 lung SCC cell lines, investigated effects and mechanisms of phosphorylated-residue-interacting sncRNAs...
March 16, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28322791/lps-depletes-phlpp-levels-in-macrophages-through-the-inhibition-of-sp1-dependent-transcriptional-regulation
#5
Neeraja P Alamuru-Yellapragada, Sanghamitra Vundyala, Soma Behera, Kishore V L Parsa
We have previously reported that bacterial endotoxin LPS attenuates expression of PHLPP, a ser/thr phosphatase, at both transcript and protein levels in different immune cells, however the underlying molecular mechanism is unknown and is of significant interest. Here, in line with the decreased transcript levels upon LPS treatment, we observed that LPS caused significant reduction in PHLPP promoter activity. We observed that SP1, a transcription factor frequently associated with inflammation, was recruited to the PHLPP promoter region...
March 18, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28317236/rehabilitation-of-revision-total-hip-replacement-a-multi-centre-survey-of-current-practice
#6
Meredith Newman, Karen Barker
BACKGROUND: Demand for revision total hip replacement (RTHR) is increasing. Outcomes after RTHR are varied, with reasonable pain relief but smaller gains in function and mobility. Whether sub-optimal rehabilitation contributes to poorer functional outcomes is unclear. Current rehabilitation is not well defined and there is little research into RTHR rehabilitation. AIMS AND OBJECTIVES: To gather information about the current rehabilitation of patients undergoing planned, single-stage revision or re-revision THR surgery...
March 20, 2017: Musculoskeletal Care
https://www.readbyqxmd.com/read/28316890/computer-modelling-reveals-new-conformers-of-the-atp-binding-loop-of-na-k-atpase-involved-in-the-transphosphorylation-process-of-the-sodium-pump
#7
Gracian Tejral, Bruno Sopko, Alois Necas, Wilhelm Schoner, Evzen Amler
Hydrolysis of ATP by Na(+)/K(+)-ATPase, a P-Type ATPase, catalyzing active Na(+) and K(+) transport through cellular membranes leads transiently to a phosphorylation of its catalytical α-subunit. Surprisingly, three-dimensional molecular structure analysis of P-type ATPases reveals that binding of ATP to the N-domain connected by a hinge to the P-domain is much too far away from the Asp(369) to allow the transfer of ATP's terminal phosphate to its aspartyl-phosphorylation site. In order to get information for how the transfer of the γ-phosphate group of ATP to the Asp(369) is achieved, analogous molecular modeling of the M4-M5 loop of ATPase was performed using the crystal data of Na(+)/K(+)-ATPase of different species...
2017: PeerJ
https://www.readbyqxmd.com/read/28303384/spinopelvic-parameter-changes-and-low-back-pain-improvement-due-to-femoral-neck-anteversion-in-patients-with-severe-unilateral-primary-hip-osteoarthritis-undergoing-total-hip-replacement
#8
Andrea Piazzolla, Giuseppe Solarino, Davide Bizzoca, Viola Montemurro, Pedro Berjano, Claudio Lamartina, Biagio Moretti
PURPOSE: The study of the interrelation between hip and spine disorders is gaining increasing importance in the last years, but the link between Hip Osteoarthritis (HOA) and Low Back Pain (LBP) remains still unclear. Aim of the study is to assess the relationship between Femoral Neck Anteversion (FNA), LBP, and spinopelvic parameters in patients undergoing Total Hip Replacement (THR) for unilateral severe primary HOA. MATERIALS AND METHODS: 91 patients were recruited...
March 16, 2017: European Spine Journal
https://www.readbyqxmd.com/read/28302767/effect-of-different-%C3%AE-subunit-isoforms-on-the-regulation-of-ampk
#9
Robin Willows, Naveenan Navaratnam, Ana Lima, Jon Read, David Carling
AMP-activated protein kinase (AMPK) plays a key role in integrating metabolic pathways in response to energy demand. AMPK activation results in a wide range of downstream responses, many of which are associated with improved metabolic outcome, making AMPK an attractive target for the treatment of metabolic diseases. AMPK is a heterotrimeric complex consisting of a catalytic subunit (α) and two regulatory subunits (β and γ). The γ subunit harbours the nucleotide binding sites and plays an important role in AMPK regulation in response to cellular energy levels...
March 16, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/28302377/stair-negotiation-as-a-rehabilitation-intervention-for-enhancing-recovery-following-total-hip-and-knee-replacement-surgery
#10
REVIEW
James P Gavin, Tikki Immins, Thomas Wainwright
BACKGROUND: Total hip replacement (THR) and total knee replacement (TKR) are common orthopaedic procedures. However, an optimal programme for post-operative rehabilitation has yet to be established. Stair negotiation is a challenging, habitual task, regularly used as a post-operative functional outcome measure; yet as a physical rehabilitation intervention it appears to be rarely used. AIM: The review purpose was to investigate the effectiveness of stair climbing as a rehabilitation intervention for THR and TKR patients...
October 20, 2016: International Journal of Orthopaedic and Trauma Nursing
https://www.readbyqxmd.com/read/28301953/glucose-variability-aggravates-cardiac-fibrosis-by-altering-akt-signalling-path
#11
Changjiang Ying, Ting Liu, Hongwei Ling, Mingyue Cheng, Xiaoyan Zhou, Shanshan Wang, Yizhen Mao, Lei Chen, Runze Zhang, Wei Li
OBJECTIVE: To study the effect of blood glucose variability on cardiac fibrosis and its mechanism in a model of diabetic cardiomyopathy. METHODS: A total of 45 Sprague Dawley rats were randomly divided into three groups: control, control diabetes mellitus and fluctuated blood glucose groups. Fluctuated blood glucose was induced by daily subcutaneous insulin and intraperitoneal glucose injections at different time points. Blood lipids and glycosylated haemoglobin A1c were assessed...
March 1, 2017: Diabetes & Vascular Disease Research
https://www.readbyqxmd.com/read/28300216/ein3-and-sos2-synergistically-modulate-plant-salt-tolerance
#12
Ruidang Quan, Juan Wang, Dexin Yang, Haiwen Zhang, Zhijin Zhang, Rongfeng Huang
Ethylene biosynthesis and the ethylene signaling pathway regulate plant salt tolerance by activating the expression of downstream target genes such as those related to ROS and Na(+)/K(+) homeostasis. The Salt Overly Sensitive (SOS) pathway regulates Na(+)/K(+) homeostasis in Arabidopsis under salt stress. However, the connection between these two pathways is unclear. Through genetic screening, we identified two sos2 alleles as salt sensitive mutants in the ein3-1 background. Neither Ethylene-Insensitive 2 (EIN2) nor EIN3 changed the expression patterns of SOS genes including SOS1, SOS2, SOS3 and SOS3-like Calcium Binding Protein 8 (SCaBP8), but SOS2 activated the expression of one target gene of EIN3, Ethylene and Salt-inducible ERF 1 (ESE1)...
March 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28298445/the-structure-and-function-of-mycobacterium-tuberculosis-mazf-mt6-provides-insights-into-conserved-features-of-mazf-endonucleases
#13
Eric D Hoffer, Stacey J Miles, Christine M Dunham
Toxin-antitoxin systems are ubiquitous in prokaryotic and archaea genomes and regulate growth in response to stress. E. coli contains at least 36 putative toxin-antitoxin gene pairs, and some pathogens such as Mycobacterium tuberculosis (Mtb) have over 90 toxin-antitoxin operons. E. coli MazF cleaves free mRNA after encountering stress and nine Mtb MazF family members cleave mRNA, tRNA, or rRNA. Moreover, Mtb MazF-mt6 cleaves 23S rRNA Helix 70 to inhibit protein synthesis. The overall tertiary folds of these MazFs are predicted to be similar, therefore it is unclear how they recognize structurally distinct RNAs...
March 15, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28296595/time-course-effect-of-r-alpha-lipoic-acid-on-cellular-metabolomics-in-cultured-hepatoma-cells
#14
Naoko Ikuta, Keita Chikamoto, Yuya Asano, Yoshiaki Yasui, Haruka Yokokawa, Keiji Terao, Gerald Rimbach, Seiichi Matsugo
Alpha-lipoic acid (LA) is a powerful antioxidant. LA has two enantiomers, R(+)-LA (R-LA) and S(-)-LA (S-LA). Of these, R-LA is naturally occurring and an essential cofactor in energy metabolism. R-LA treatment has been reported to affect glucose metabolism in rat hepatoma cells. This study analyzed the time course of metabolite levels in LA-treated cultured H4IIEC3 rat hepatoma cells, including a specific evaluation of the effect of R-LA and the enantioselectivity of LA. Principal component analysis showed that this experiment was well designed to observe enantioselectivity...
March 2017: Journal of Medicinal Food
https://www.readbyqxmd.com/read/28296566/a-new-elisa-based-method-for-detection-of-o-glcnac-transferase-activity-in-vitro
#15
Jieqiong Qi, Ruihong Wang, Yazhen Zeng, Wengong Yu, Yuchao Gu
O-GlcNAcylation is a dynamic, reversible, post-translational modification that regulates many cellular processes. O-GlcNAc transferase (OGT) is the sole enzyme transferring N-acetylglucosamine from UDP-GlcNAc to selected Ser/Thr residues of cytoplasm and nucleus proteins. Aberrant of OGT activity is associated with a number of diseases, suggesting OGT as a novel therapeutic target. In this study, we created a new ELISA-based method for detection of OGT activity. First, CKII, a well-known OGT substrate, was coated onto ELISA plate...
March 15, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28293842/analysis-of-ethylene-receptors-assay-for-histidine-kinase-activity
#16
G Eric Schaller, Brad M Binder
The ethylene receptors of plants exist in two subfamilies. Members of subfamily-1 have functional histidine kinase domains, whereas members of subfamily-2 have diverged histidine-kinase-like domains that in some cases have been shown to exhibit Ser/Thr kinase activity. Here, we describe a method to biochemically characterize the enzymatic activity of these kinase domains in vitro. For this purpose, the histidine kinase domain of the receptors is transgenically expressed in yeast as a fusion to glutathione-S-transferase (GST) for subsequent affinity purification...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28289588/mutational-analysis-of-mitochondrial-trna-genes-in-patients-with-lung-cancer
#17
Z F He, L C Zheng, D Y Xie, S S Yu, J Zhao
Mutations in mitochondrial tRNA (mt-tRNA) genes have been found to be associated with various diseases including lung cancer. To understand the possible relationship between mtRNA mutations and lung cancer, we sequenced the 22 mt-tRNA genes from 200 lung cancer blood samples, as well as 100 healthy subjects. As a result, five mutations were identified including the tRNA(Ala) T5655C, tRNA(Arg) T10454C, tRNA(Leu(CUN)) A12330G, tRNA(Ser(UCN)) T7505C and tRNA(Thr) G15927A. These mutations were absent in the healthy subjects...
December 1, 2016: Balkan Journal of Medical Genetics: BJMG
https://www.readbyqxmd.com/read/28286463/range-of-movement-for-impingement-and-dislocation-avoidance-in-total-hip-replacement-predicted-by-finite-element-model
#18
Laura Ezquerra, María Paz Quilez, María Ángeles Pérez, Jorge Albareda, Belén Seral
Dislocation is a serious complication in total hip replacement (THR). An inadequate range of movement (ROM) can lead to impingement of the prosthesis neck on the acetabular cup; furthermore, the initiation of subluxation and dislocation may occur. The objective of this study was to generate a parametric three-dimensional finite element (FE) model capable of predicting the dislocation stability for various positions of the prosthetic head, neck, and cup under various activities. Three femoral head sizes (28, 32, and 36 mm) were simulated...
2017: Journal of Medical and Biological Engineering
https://www.readbyqxmd.com/read/28279171/single-amino-acid-substitutions-in-the-selectivity-filter-render-nbxip1-1%C3%AE-aquaporin-water-permeable
#19
Henry Ampah-Korsah, Yonathan Sonntag, Angelica Engfors, Andreas Kirscht, Per Kjellbom, Urban Johanson
BACKGROUND: Aquaporins (AQPs) are integral membrane proteins that facilitate transport of water and/or other small neutral solutes across membranes in all forms of life. The X Intrinsic Proteins (XIPs) are the most recently recognized and the least characterized aquaporin subfamily in higher plants. XIP1s have been shown to be impermeable to water but permeable to boric acid, glycerol, hydrogen peroxide and urea. However, uncertainty regarding the determinants for selectivity and lack of an activity that is easy to quantify have hindered functional investigations...
March 9, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28276468/nv-proteins-of-fish-novirhabdovirus-recruit-cellular-ppm1bb-protein-phosphatase-and-antagonize-rig-i-mediated-ifn-induction
#20
Stéphane Biacchesi, Emilie Mérour, Didier Chevret, Annie Lamoureux, Julie Bernard, Michel Brémont
Non virion (NV) protein expression is critical for fish Novirhabdovirus, viral hemorrhagic septicemia virus (VHSV) and infectious hematopoietic necrosis virus (IHNV), in vivo pathogenesis. However, the mechanism by which NV promotes the viral replication is still unclear. We developed an approach based on reverse genetics and interactomic and identified several NV-associated cellular partners underlying cellular pathways as potential viral targets. Among these cell partners, we showed that NV proteins specifically interact with a protein phosphatase, Mg(2+)/Mn(2+)-dependent, 1Bb (PPM1Bb) and recruit it in the close vicinity of mitochondria, a subcellular compartment important for retinoic acid-inducible gene-I- (RIG-I)-mediated interferon induction pathway...
March 9, 2017: Scientific Reports
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