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CHARLES E. MURRY

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https://www.readbyqxmd.com/read/28684531/cardiomyocyte-regeneration-a-consensus-statement
#1
Thomas Eschenhagen, Roberto Bolli, Thomas Braun, Loren J Field, Bernd K Fleischmann, Jonas Frisén, Mauro Giacca, Joshua M Hare, Steven R Houser, Richard T Lee, Eduardo Marbán, James F Martin, Jeffery D Molkentin, Charles E Murry, Paul R Riley, Pilar Ruiz-Lozano, Hesham A Sadek, Mark A Sussman, Joseph A Hill
Cell therapy is an exciting option for repairing the injured heart, one which has attracted considerable interest over the past 15 years. Consensus exists that the injection/infusion or tissue-based implantation of various cell types may exert therapeutic effects, and there is general agreement that additional molecular, translational and clinical studies are required to define the optimal cell source, method of delivery, and underlying mechanism(s) of action.One of the remaining questions in this field pertains to cardiomyocyte turnover under normal and diseased conditions and its contribution to the beneficial effects of cell therapy...
July 6, 2017: Circulation
https://www.readbyqxmd.com/read/28634611/micro-and-nano-patterned-conductive-graphene-peg-hybrid-scaffolds-for-cardiac-tissue-engineering
#2
Alec S T Smith, Hyok Yoo, Hyunjung Yi, Eun Hyun Ahn, Justin H Lee, Guozheng Shao, Ekaterina Nagornyak, Michael A Laflamme, Charles E Murry, Deok-Ho Kim
A lack of electrical conductivity and structural organization in currently available biomaterial scaffolds limits their utility for generating physiologically representative models of functional cardiac tissue. Here we report on the development of scalable, graphene-functionalized topographies with anisotropic electrical conductivity for engineering the structural and functional phenotypes of macroscopic cardiac tissue constructs. Guided by anisotropic electroconductive and topographic cues, the tissue constructs displayed structural property enhancement in myofibrils and sarcomeres, and exhibited significant increases in the expression of cell-cell coupling and calcium handling proteins, as well as in action potential duration and peak calcium release...
June 29, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28553667/translation-of-cardiac-myosin-activation-with-2-deoxy-atp-to-treat-heart-failure-via-an-experimental-ribonucleotide-reductase-based-gene-therapy
#3
Kassandra S Thomson, Guy L Odom, Charles E Murry, Gregory G Mahairas, Farid Moussavi-Harami, Sam L Teichman, Xiaolan Chen, Stephen D Hauschka, Jeffrey S Chamberlain, Michael Regnier
Despite recent advances, chronic heart failure remains a significant and growing unmet medical need, reaching epidemic proportions carrying substantial morbidity, mortality, and costs. A safe and convenient therapeutic agent that produces sustained inotropic effects could ameliorate symptoms, and improve functional capacity and quality of life. We discovered small amounts of 2-deoxy-ATP (dATP) activate cardiac myosin leading to enhanced contractility in normal and failing heart muscle. Cardiac myosin activation triggers faster myosin crossbridge cycling with greater force generation during each contraction...
December 2016: JACC. Basic to Translational Science
https://www.readbyqxmd.com/read/28483831/one-stride-forward-maturation-and-scalable-production-of-engineered-human-myocardium
#4
EDITORIAL
Xiulan Yang, Charles E Murry
No abstract text is available yet for this article.
May 9, 2017: Circulation
https://www.readbyqxmd.com/read/28065644/in%C3%A2-vivo-maturation-of-human-induced-pluripotent-stem-cell-derived-cardiomyocytes-in-neonatal-and-adult-rat-hearts
#5
Shin Kadota, Lil Pabon, Hans Reinecke, Charles E Murry
We hypothesized that the neonatal rat heart would bring transplanted human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) to maturity as it grows to adult size. In neonatal rat heart, engrafted hiPSC derivatives developed partially matured myofibrils after 3 months, with increasing cell size and sarcomere length. There was no difference between grafts from hiPSC-CMs or hiPSC-derived cardiac progenitors (hiPSC-CPs) at 3 months, nor was maturation influenced by infarction. Interestingly, the infarcted adult heart induced greater human cardiomyocyte hypertrophy and induction of cardiac troponin I expression than the neonatal heart...
February 14, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/27993159/erratum-to-statistically-based-splicing-detection-reveals-neural-enrichment-and-tissue-specific-induction-of-circular-rna-during-human-fetal-development
#6
Linda Szabo, Robert Morey, Nathan J Palpant, Peter L Wang, Nastaran Afari, Chuan Jiang, Mana M Parast, Charles E Murry, Louise C Laurent, Julia Salzman
No abstract text is available yet for this article.
December 19, 2016: Genome Biology
https://www.readbyqxmd.com/read/27906170/generating-high-purity-cardiac-and-endothelial-derivatives-from-patterned-mesoderm-using-human-pluripotent-stem-cells
#7
Nathan J Palpant, Lil Pabon, Clayton E Friedman, Meredith Roberts, Brandon Hadland, Rebecca J Zaunbrecher, Irwin Bernstein, Ying Zheng, Charles E Murry
Human pluripotent stem cells (hPSCs) provide a valuable model for the study of human development and a means to generate a scalable source of cells for therapeutic applications. This protocol specifies cell fate efficiently into cardiac and endothelial lineages from hPSCs. The protocol takes 2 weeks to complete and requires experience in hPSC culture and differentiation techniques. Building on lessons taken from early development, this monolayer-directed differentiation protocol uses different concentrations of activin A and bone morphogenetic protein 4 (BMP4) to polarize cells into mesodermal subtypes that reflect mid-primitive-streak cardiogenic mesoderm and posterior-primitive-streak hemogenic mesoderm...
January 2017: Nature Protocols
https://www.readbyqxmd.com/read/27737958/mechanical-stress-conditioning-and-electrical-stimulation-promote-contractility-and-force-maturation-of-induced-pluripotent-stem-cell-derived-human-cardiac-tissue
#8
Jia-Ling Ruan, Nathaniel L Tulloch, Maria V Razumova, Mark Saiget, Veronica Muskheli, Lil Pabon, Hans Reinecke, Michael Regnier, Charles E Murry
BACKGROUND: Tissue engineering enables the generation of functional human cardiac tissue with cells derived in vitro in combination with biocompatible materials. Human-induced pluripotent stem cell-derived cardiomyocytes provide a cell source for cardiac tissue engineering; however, their immaturity limits their potential applications. Here we sought to study the effect of mechanical conditioning and electric pacing on the maturation of human-induced pluripotent stem cell-derived cardiac tissues...
November 15, 2016: Circulation
https://www.readbyqxmd.com/read/27716639/depth-resolved-3d-visualization-of-coronary-microvasculature-with-optical-microangiography
#9
Wan Qin, Meredith A Roberts, Xiaoli Qi, Charles E Murry, Ying Zheng, Ruikang K Wang
In this study, we propose a novel implementation of optical coherence tomography-based angiography combined with ex vivo perfusion of fixed hearts to visualize coronary microvascular structure and function. The extracorporeal perfusion of Intralipid solution allows depth-resolved angiographic imaging, control of perfusion pressure, and high-resolution optical microangiography. The imaging technique offers new opportunities for microcirculation research in the heart, which has been challenging due to motion artifacts and the lack of independent control of pressure and flow...
November 7, 2016: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/27600722/real-time-force-and-frequency-analysis-of-engineered-human-heart-tissue-derived-from-induced-pluripotent-stem-cells-using-magnetic-sensing
#10
Kevin S Bielawski, Andrea Leonard, Shiv Bhandari, Chuck E Murry, Nathan J Sniadecki
Engineered heart tissues made from human pluripotent stem cell-derived cardiomyocytes have been used for modeling cardiac pathologies, screening new therapeutics, and providing replacement cardiac tissue. Current methods measure the functional performance of engineered heart tissue by their twitch force and beating frequency, typically obtained by optical measurements. In this article, we describe a novel method for assessing twitch force and beating frequency of engineered heart tissue using magnetic field sensing, which enables multiple tissues to be measured simultaneously...
October 2016: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/27503462/prosurvival-factors-improve-functional-engraftment-of-myogenically-converted-dermal-cells-into-dystrophic-skeletal-muscle
#11
Lindsey A Muir, Charles E Murry, Jeffrey S Chamberlain
In Duchenne muscular dystrophy (DMD) and other muscle wasting disorders, cell therapies are a promising route for promoting muscle regeneration by supplying a functional copy of the missing dystrophin gene and contributing new muscle fibers. The clinical application of cell-based therapies is resource intensive, and it will therefore be necessary to address key limitations that reduce cell engraftment into muscle tissue. A pressing issue is poor donor cell survival following transplantation, which in preclinical studies limits the ability to effectively test the impact of cell-based therapy on whole muscle function...
September 7, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27280684/distilling-complexity-to-advance-cardiac-tissue-engineering
#12
REVIEW
Brenda M Ogle, Nenad Bursac, Ibrahim Domian, Ngan F Huang, Philippe Menasché, Charles E Murry, Beth Pruitt, Milica Radisic, Joseph C Wu, Sean M Wu, Jianyi Zhang, Wolfram-Hubertus Zimmermann, Gordana Vunjak-Novakovic
The promise of cardiac tissue engineering is in the ability to recapitulate in vitro the functional aspects of a healthy heart and disease pathology as well as to design replacement muscle for clinical therapy. Parts of this promise have been realized; others have not. In a meeting of scientists in this field, five central challenges or "big questions" were articulated that, if addressed, could substantially advance the current state of the art in modeling heart disease and realizing heart repair.
June 8, 2016: Science Translational Medicine
https://www.readbyqxmd.com/read/27193661/policy-global-standards-for-stem-cell-research
#13
Jonathan Kimmelman, Insoo Hyun, Nissim Benvenisty, Timothy Caulfield, Helen E Heslop, Charles E Murry, Douglas Sipp, Lorenz Studer, Jeremy Sugarman, George Q Daley
No abstract text is available yet for this article.
May 19, 2016: Nature
https://www.readbyqxmd.com/read/27185282/setting-global-standards-for-stem-cell-research-and-clinical-translation-the%C3%A2-2016-isscr-guidelines
#14
George Q Daley, Insoo Hyun, Jane F Apperley, Roger A Barker, Nissim Benvenisty, Annelien L Bredenoord, Christopher K Breuer, Timothy Caulfield, Marcelle I Cedars, Joyce Frey-Vasconcells, Helen E Heslop, Ying Jin, Richard T Lee, Christopher McCabe, Megan Munsie, Charles E Murry, Steven Piantadosi, Mahendra Rao, Heather M Rooke, Douglas Sipp, Lorenz Studer, Jeremy Sugarman, Masayo Takahashi, Mark Zimmerman, Jonathan Kimmelman
The International Society for Stem Cell Research (ISSCR) presents its 2016 Guidelines for Stem Cell Research and Clinical Translation (ISSCR, 2016). The 2016 guidelines reflect the revision and extension of two past sets of guidelines (ISSCR, 2006; ISSCR, 2008) to address new and emerging areas of stem cell discovery and application and evolving ethical, social, and policy challenges. These guidelines provide an integrated set of principles and best practices to drive progress in basic, translational, and clinical research...
June 14, 2016: Stem Cell Reports
https://www.readbyqxmd.com/read/27179752/new-isscr-guidelines-clinical-translation-of-stem-cell-research
#15
Jonathan Kimmelman, Helen E Heslop, Jeremy Sugarman, Lorenz Studer, Nissim Benvenisty, Timothy Caulfield, Insoo Hyun, Charles E Murry, Douglas Sipp, George Q Daley
No abstract text is available yet for this article.
May 14, 2016: Lancet
https://www.readbyqxmd.com/read/27174977/scientific-community-confronting-stem-cell-hype
#16
Timothy Caulfield, Douglas Sipp, Charles E Murry, George Q Daley, Jonathan Kimmelman
No abstract text is available yet for this article.
May 13, 2016: Science
https://www.readbyqxmd.com/read/27161364/isolation-and-mechanical-measurements-of-myofibrils-from-human-induced-pluripotent-stem-cell-derived-cardiomyocytes
#17
Josè Manuel Pioner, Alice W Racca, Jordan M Klaiman, Kai-Chun Yang, Xuan Guan, Lil Pabon, Veronica Muskheli, Rebecca Zaunbrecher, Jesse Macadangdang, Mark Y Jeong, David L Mack, Martin K Childers, Deok-Ho Kim, Chiara Tesi, Corrado Poggesi, Charles E Murry, Michael Regnier
Tension production and contractile properties are poorly characterized aspects of excitation-contraction coupling of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). Previous approaches have been limited due to the small size and structural immaturity of early-stage hiPSC-CMs. We developed a substrate nanopatterning approach to produce hiPSC-CMs in culture with adult-like dimensions, T-tubule-like structures, and aligned myofibrils. We then isolated myofibrils from hiPSC-CMs and measured the tension and kinetics of activation and relaxation using a custom-built apparatus with fast solution switching...
June 14, 2016: Stem Cell Reports
https://www.readbyqxmd.com/read/27058929/imprecision-medicine-a-one-size-fits-many-approach-for-muscle-dystrophy
#18
COMMENT
Astrid Breitbart, Charles E Murry
There is still no curative treatment for Duchenne muscular dystrophy (DMD). In this issue of Cell Stem Cell, Young et al. (2016) demonstrate a genome editing approach applicable to 60% of DMD patients with CRISPR/Cas9 using one pair of guide RNAs.
April 7, 2016: Cell Stem Cell
https://www.readbyqxmd.com/read/26955856/stromal-cells-in-dense-collagen-promote-cardiomyocyte-and-microvascular-patterning-in-engineered-human-heart-tissue
#19
Meredith A Roberts, Dominic Tran, Kareen L K Coulombe, Maria Razumova, Michael Regnier, Charles E Murry, Ying Zheng
Cardiac tissue engineering is a strategy to replace damaged contractile tissue and model cardiac diseases to discover therapies. Current cardiac and vascular engineering approaches independently create aligned contractile tissue or perfusable vasculature, but a combined vascularized cardiac tissue remains to be achieved. Here, we sought to incorporate a patterned microvasculature into engineered heart tissue, which balances the competing demands from cardiomyocytes to contract the matrix versus the vascular lumens that need structural support...
April 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/26866596/nanotopography-induced-structural-anisotropy-and-sarcomere-development-in-human-cardiomyocytes-derived-from-induced-pluripotent-stem-cells
#20
Daniel Carson, Marketa Hnilova, Xiulan Yang, Cameron L Nemeth, Jonathan H Tsui, Alec S T Smith, Alex Jiao, Michael Regnier, Charles E Murry, Candan Tamerler, Deok-Ho Kim
Understanding the phenotypic development of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) is a prerequisite to advancing regenerative cardiac therapy, disease modeling, and drug screening applications. Lack of consistent hiPSC-CM in vitro data can be largely attributed to the inability of conventional culture methods to mimic the structural, biochemical, and mechanical aspects of the myocardial niche accurately. Here, we present a nanogrid culture array comprised of nanogrooved topographies, with groove widths ranging from 350 to 2000 nm, to study the effect of different nanoscale structures on the structural development of hiPSC-CMs in vitro...
August 31, 2016: ACS Applied Materials & Interfaces
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