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https://www.readbyqxmd.com/read/28627855/multiplex-pcr-gastro-intestinal-panels-for-the-management-of-traveller-s-diarrhea-are-they-accurate-and-useful
#1
Laurence Rochat, Antony Croxatto, Serge De Vallière, Valérie D'Acremont, Blaise Genton
Until recently, the search for enteropathogens causing travellers' diarrhea was based on stool culture (Campylobacter spp., Salmonella spp. and Shigella spp.), direct microscopy with (Cryptosporidium spp.) or without specific staining (Giardia lamblia, Entamoeba histolytica) or specific antigen detection (Giardia lamblia, Entamoeba histolytica). Molecular analyses are progressively replacing traditional diagnostic methods but their clinical usefulness remains to be better defined. This article attempts to describe the advantages and disadvantages of these new molecular methods and to illustrate situations where they could be useful using clinical cases frequently encountered in the practice of travel medicine...
May 3, 2017: Revue Médicale Suisse
https://www.readbyqxmd.com/read/28624121/leptin-like-immunoreactivity-in-the-central-nervous-system-digestive-organs-and-gonads-of-the-giant-freshwater-prawn-macrobrachium-rosenbergii
#2
Jaruwan Poljaroen, Yotsawan Tinikul, Ruchanok Tinikul, Panat Anurucpreeda, Prasert Sobhon
Leptin, a highly conserved adipocyte-derived hormone, plays important roles in a variety of physiological processes, including the control of fat storage and metabolic status which are linked to food intake, energy homeostasis, and reproduction in all vertebrates. In the present study, we hypothesize that leptin is also present in various organs of the fresh water prawns, Macrobrachium rosenbergii. The existence and distribution of a leptin-like peptide in prawn tissues were verified by using Western blotting (WB) and immunohistochemical detection (ID) using primary antibody against human leptin...
June 14, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28621952/gastrointestinal-and-systemic-disposition-of-diclofenac-under-fasted-and-fed-state-conditions-supporting-the-evaluation-of-in-vitro-predictive-tools
#3
Jens Van Den Abeele, Ronald Schilderink, FElix Schneider, Raf Mols, Mans Minekus, Werner Weitschies, Joachim Brouwers, Jan Tack, Patrick Augustijns
This study aimed to gain further insight into the gastrointestinal disposition of the weakly acidic BCS Class II drug diclofenac and the implications for systemic drug exposure in humans under fasted and fed state conditions. For this purpose, gastrointestinal and blood samples were collected from healthy volunteers after oral intake of a commercially available tablet of the potassium salt of diclofenac (i.e. Cataflam®) in different prandial states. Subsequently, these in vivo data served as a reference for the evaluation of in vitro tools with different levels of complexity, i...
June 16, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28617330/identification-of-pathogenic-factors-in-klebsiella-pneumoniae-using-impedimetric-sensor-equipped-with-biomimetic-surfaces
#4
Duyen Thi Ngoc Huynh, Ah-Young Kim, Young-Rok Kim
K.pneumoniae is an opportunistic pathogen that causes nosocomial infections, such as, pneumonia, urinary tract infections, septic shock, and gastro intestinal disease. Lipopolysaccharide (LPS), capsular polysaccharide, and fimbriae are recognized major virulence factors of K.pneumoniae and play key roles during early stages of infections. In this study, we functionalized the surface of gold electrode with mannose and mucin to monitor the adhesion-associated virulence factors of K.pneumoniae. The binding characteristics of K...
June 15, 2017: Sensors
https://www.readbyqxmd.com/read/28615864/prevalence-of-gastro-intestinal-parasites-in-pigs-in-punjab-india
#5
Mandeep Kaur, Balbir Bagicha Singh, Rajnish Sharma, Jatinder Paul Singh Gill
Gastrointestinal parasites are a common problem in pigs in India. The important risk factors include coprophagic behaviour of pigs and their free access to garbage. To investigate the gastrohelmenthic spectrum in pigs of Punjab, we examined 265 faecal samples from farm (n = 47) and scavenging pigs (n = 218) using faecal floatation method. Ascaris suum, unsporulated oocysts, Trichuris spp. and Strongyloides were recorded in 27.5, 15.4, 1.8 and 4.5 % of the pig faecal samples, respectively. Overall prevalence was significantly higher in pigs >1 year (56...
June 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28615851/gastrointestinal-helminthosis-prevalence-and-associated-determinants-in-goats-of-jabalpur-india
#6
Alok Kumar Dixit, Giridhari Das, Ramesh Pratap Singh Baghel
A total of 632 faecal samples of goats of Jabalpur district of Madhya Pradesh state were examined during three years of the study period (November 2012-October 2015), out of which 82.75 % samples were positive for helminthic infections. Among various helminths, maximum prevalence was of strongyles (32.59 %) followed by amphistomes (14.40 %), Moniezia spp. (12.50 %), Strongyloides spp. (0.79 %), Trichuris spp. (0.47 %) and Fasciolagigantica (0.32 %). Year wise prevalence revealed a highly significant (p < 0...
June 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28615071/survivin-expression-pattern-in-the-intestine-of-normoxic-and-ischemic-rats
#7
Alexandra Scheer, Shirley K Knauer, Rabea Verhaegh
BACKGROUND: Survivin, a member of the inhibitor of apoptosis protein (IAP) family, regulates mitosis and chromosome segregation. The expression of survivin proceeds during embryonic development and in addition has already been demonstrated in cancer cells. However, there is also evidence of survivin expression in differentiated tissues, including the gastro-intestinal tract of adult rats. A study with human colon specimens exhibited survivin in most basal crypt epithelial cells of normal mucosa...
June 14, 2017: BMC Gastroenterology
https://www.readbyqxmd.com/read/28614859/gastro-intestinal-ultrasound-much-has-long-been-known-and-proven
#8
K Seitz, S Ödegaard, H Lutz
No abstract text is available yet for this article.
June 2017: Ultraschall in der Medizin
https://www.readbyqxmd.com/read/28605168/characteristics-and-management-of-iga-vasculitis-henoch-sch%C3%A3-nlein-purpura-in-adults-data-from-the-260-patients-included-in-the-igavas-survey
#9
Alexandra Audemard-Verger, Benjamin Terrier, Agnès Dechartres, Johan Chanal, Zahir Amoura, Noémie Le Gouellec, Patrice Cacoub, Noémie Jourde-Chiche, Geoffrey Urbanski, Jean-François Augusto, Guillaume Moulis, Loic Raffray, Alban Deroux, Aurélie Hummel, Bertrand Lioger, Mélanie Catroux, Stanislas Faguer, Julie Goutte, Nihal Martis, François Maurier, Etienne Rivière, Sébastien Sanges, Aurélie Baldolli, Nathalie Costedoat-Chalumeau, Mélanie Roriz, Xavier Puéchal, Marc André, Christian Lavigne, Boris Bienvenu, Arsène Mekinian, Elie Zagdoun, Charlotte Girard, Alice Bérezné, Loïc Guillevin, Eric Thervet, Evangéline Pillebout
OBJECTIVES: Data on adult IgA vasculitis (IgAV) are scarce. This survey was designed to better define clinical spectrum and efficacy of treatments in this population. METHODS: We analyzed data from 260 patients with IgAV included in a French multicenter retrospective survey. RESULTS: Mean age at diagnosis was 50.1±18 years, and 63% were male. Baseline manifestations included purpura (100%), arthralgia (62%), glomerulonephritis (70%) or gastro-intestinal involvement (53%)...
June 12, 2017: Arthritis & Rheumatology
https://www.readbyqxmd.com/read/28602545/what-is-the-impact-of-neoadjuvant-chemoradiation-on-outcomes-in-gastro-intestinal-cancer
#10
C Mariette, A Brouquet, D Tzanis, A Laurenzi, A de la Rochefordière, P Mariani, G Piessen, A Sa Cunha, C Penna
Multimodal therapeutic strategies combining chemotherapy, radiation therapy and surgery have been shown to be feasible and to have a positive impact on outcomes by decreasing the risk of locoregional recurrence and often by increasing overall survival. The advantages of neoadjuvant chemo(radio)therapy include optimal tumor control combined with better tolerance and compliance to treatment while also increasing the number of candidates for surgery. Whereas indications for neoadjuvant therapy are increasing, its impact on surgical treatment and postoperative outcomes are not well-known...
June 8, 2017: Journal of Visceral Surgery
https://www.readbyqxmd.com/read/28600084/modelling-duodenum-radiotherapy-toxicity-using-cohort-dose-volume-histogram-data
#11
Daniel L P Holyoake, Marianne Aznar, Somnath Mukherjee, Mike Partridge, Maria A Hawkins
BACKGROUND AND PURPOSE: Gastro-intestinal toxicity is dose-limiting in abdominal radiotherapy and correlated with duodenum dose-volume parameters. We aimed to derive updated NTCP model parameters using published data and prospective radiotherapy quality-assured cohort data. MATERIAL AND METHODS: A systematic search identified publications providing duodenum dose-volume histogram (DVH) statistics for clinical studies of conventionally-fractionated radiotherapy. Values for the Lyman-Kutcher-Burman (LKB) NTCP model were derived through sum-squared-error minimisation and using leave-one-out cross-validation...
June 6, 2017: Radiotherapy and Oncology: Journal of the European Society for Therapeutic Radiology and Oncology
https://www.readbyqxmd.com/read/28598564/withdrawn-celecoxib-for-rheumatoid-arthritis
#12
REVIEW
Sarah E Garner, Dogan Fidan, Ruth R Frankish, Maria Judd, Beverley Shea, Tanveer Towheed, Peter Tugwell, George A Wells
BACKGROUND: Rheumatoid arthritis (RA) is a systemic auto-immune disorder, involving persistent joint inflammation. NSAIDs are used to control the symptoms of RA, but are associated with significant gastro-intestinal toxicity, including a risk of potentially life threatening gastroduodenal perforations, ulcers and bleeds. The NSAIDs known as the selective Cox II inhibitors, of which celecoxib is a member, were developed in order to reduce the GI toxicity, but are more expensive. OBJECTIVES: To establish the efficacy and safety of celecoxib in the management of RA by systematic review of available evidence...
June 9, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28598064/emphysematous-gastritis-with-air-in-portal-venous-system
#13
Omesh Goyal, Ajesh Bansal, Prerna Goyal
Emphysematous gastritis is a rare but severe form of phlegmonous gastritis caused by gastric mucosal disruption and infection of stomach wall by gas-forming bacteria. Ingestion of corrosive substances is the most common predisposing factor, followed by alcohol abuse, abdominal surgery, diabetes and immunosuppression. Patients usually present with abdominal pain, vomiting, diarrhea, constipation and/or gastro-intestinal hemorrhage. Characteristic radiological findings include presence of gas in the gastric wall...
May 2017: Journal of the Association of Physicians of India
https://www.readbyqxmd.com/read/28584741/adverse-drug-events-in-patients-with-mental-disorder-in-an-ambulatory-setting
#14
Naveen Kumar, Sangeeta Sharma, Vinod Kapoor
BACKGROUND: Although adverse drug events (ADEs) among inpatients occur frequently and are widely studied, few data are available on ADEs among outpatients with mental disorders. AIMS: To determine the rates, types, and severity of ADEs in patients with mental disorder. MATERIALS AND METHODS: Cross-sectional survey of patients with mental disorder attending outpatient department. Data were collected over a period of 6 months. RESULTS: A total of 400 patients (217 schizophrenia patients, 127 bipolar affective disorder patients, and 56 patients of depression) with a mean age of 32...
April 2017: International Journal of Applied and Basic Medical Research
https://www.readbyqxmd.com/read/28582812/symptomatic-efficacy-of-buckwheat-products-in-non-celiac-gluten-sensitivity-ncgs
#15
Monicau Dinu, Donatella Macchia, Giuditta Pagliai, Anna Maria Gori, Francesca Cesari, Rossella Marcucci, Francesco Sofi, Alessandro Casini
BACKGROUND AND OBJECTIVES: Buckwheat (Fagopyrum esculentum) is a gluten-free grain with acclaimed benefi-cial effects on human health. Our aim was to assess the effect of buckwheat products on intestinal/extra-intestinal symptoms and biochemical parameters in patients with Non-Celiac Gluten Sensitivity (NCGS). METHODS AND STUDY DESIGN: A randomized, crossover trial with two intervention phases was conducted on 19 NCGS patients over a 12 week-period. The participants were assigned to consume products made from buckwheat or to maintain their normal gluten-free diet for 6 weeks in a random order...
2017: Asia Pacific Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/28581883/discovery-and-characterization-of-a-novel-humanized-anti-il-15-antibody-and-its-relevance-for-the-treatment-of-refractory-celiac-disease-and-eosinophilic-esophagitis
#16
Alain P Vicari, Alain M Schoepfer, Bertrand Meresse, Laurence Goffin, Olivier Léger, Soheila Josserand, Nicolas Guégan, Shida Yousefi, Alex Straumann, Nadine Cerf-Bensussan, Hans-Uwe Simon, Yolande Chvatchko
Interleukin-15 (IL-15) is a critical regulator of immune responses, especially at mucosal interfaces within the gastro-intestinal tract. Here, we describe the discovery and characterization of a humanized antibody to IL-15. Data from its epitope and mode of action, cell biology and primate pharmacology, as well as translational studies in human samples and in vivo proof-of-concept experiments in mouse models demonstrate the therapeutic potential of this new antibody targeting IL-15 for refractory celiac disease and eosinophilic esophagitis...
June 5, 2017: MAbs
https://www.readbyqxmd.com/read/28576282/-evidence-of-antihypertensive-effect-of-a-land-snail-helix-aspersa-by-product-hydrolysate%C3%A2-%C3%A2-identification-of-involved-peptides
#17
P Drevet
The antihypertensive potential of a land snail by-product hydrolysate, obtained after an agri-food processing of the raw material, was studied in vitro and in vivo. First, the ACE inhibitory activity of hydrolysates obtained before and after an ultrafiltration step with a 10kDa membrane cutoff was assayed. The IC50 obtained were of 98.3μg·mL(-1) and 23μg·mL(-1), respectively, showing a 4.2 fold increase in ACE inhibitory capacity after the ultrafiltration step. Then, ACE inhibitory capacity of the hydrolysate was followed...
May 30, 2017: Annales de Cardiologie et D'angéiologie
https://www.readbyqxmd.com/read/28574214/exocrine-secretion-spelled-with-a-captial-k-bk
#18
Jens Leipziger
Exocrine secretion of NaCl follows the "pump-leak" mechanism of active salt movement across an epithelial barrier followed by the movement of osmotically obliged water. Glandular exocrine secretion of NaCl rich fluids is found in most exocrine glands of the gastro-intestinal tract, the tear and sweat glands and also in airway epithelium This article is protected by copyright. All rights reserved.
June 2, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28571209/pseudo-aneurysm-of-aberrant-right-gastroepiploic-artery-mimicking-pseudocyst-of-pancreas-a-rare-case-report
#19
Abhinav Jauhari, Ambuj Shrivastava, Rajesh Kumar Soni, Ashutosh Darbari
Pseudoaneurysm of peripancreatic vessel is a rare complication of pancreatitis. The splenic artery is the most commonly affected vessels close to the pancreas. Pseudoaneurysm of right gastroepiploic artery is rare. It can rupture into Gastro Intestinal (GI) tract, retroperitoneum, peritoneal cavity, biliopancreatic duct or pseudocyst. Clinical diagnosis of unruptured pseudoaneurysm is rarely possible because there are no specific signs and symptoms. We hereby report a case of pseudoaneurysm of aberrant right gastroepiploic artery in 30-year-old male which presented with history of epigastric pain and lump with episode of haematemesis and malena...
April 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28569454/antibiotic-treatment-to-prevent-post-extraction-complications-a-monocentric-randomized-clinical-trial-preliminary-outcomes
#20
Antonio Barone, Francesco S Marchionni, Chiara Cinquini, Andrea Cipolli Panattoni, Paolo Toti, Simone Marconcini, Ugo Covani, Mario Gabriele
BACKGROUND: Tooth extraction is a very common procedure in oral surgery. Despite this, very little information is available in the literature as to the antibiotic management of the patient. The aim of this study is to evaluate whether the antibiotic prophylaxis could be beneficial in preventing post- extraction local complications and whether the use of a probiotic could help reduce the antibiotic gastro-intestinal side effects. MATERIALS AND METHODS: 111 patients meeting the inclusion criteria were initially included in this randomized clinical trial and randomly allocated to one of the three experimental groups according to a computer-generated randomization list...
May 31, 2017: Minerva Stomatologica
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