keyword
MENU ▼
Read by QxMD icon Read
search

Human acid base

keyword
https://www.readbyqxmd.com/read/28821128/biomimetic-composite-scaffolds-based-on-surface-modification-of-polydopamine-on-electrospun-poly-lactic-acid-cellulose-nanofibrils
#1
Zhangqiang Yang, Junhui Si, Zhixiang Cui, Jianhua Ye, Xiaofeng Wang, Qianting Wang, Kaiping Peng, Wenzhe Chen, Shia-Chung Chen
An appropriate surface chemical property is crucial in tissue engineering scaffolds, which promotes cell attachment and proliferation. A biomimetic composite scaffold with a polydopamine (PDA) coating layer on electrospun poly(lactic acid) (PLA)/cellulose nanofibrils (CNF) composite nanofiber was developed in this study. PLA/CNF composite nanofibers were fabricated and then coated via treatment with a dopamine solution. The PDA coating layer was successfully formed on the surface of the PLA/CNF composite nanofiber by using a simple, environment-friendly, and effective procedure...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28820579/site-specific-conjugation-to-native-and-engineered-lysines-in-human-immunoglobulins-by-microbial-transglutaminase
#2
Jared Spidel, Benjamin Vaessen, Earl Albone, Xin Cheng, Arielle Verdi, J Bradford Kline
The use of microbial transglutaminase (MTG) to produce site-specific antibody-drug conjugates (ADCs) has thus far focused on transamidation of engineered acyl donor glutamine residues in an antibody based on the hypothesis that the lower specificity of MTG for acyl acceptor lysines may result in transamidation of multiple native lysine residues, thereby yielding heterogeneous products. We investigated the utilization of native IgG lysines as acyl acceptor sites for glutamine-based acyl donor substrates. Of the approximately 80 lysines in multiple recombinant IgG monoclonal antibodies (mAbs), none were transamidated...
August 18, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28820469/a-third-generation-glucose-biosensor-based-on-cellobiose-dehydrogenase-immobilized-on-a-glassy-carbon-electrode-decorated-with-electrodeposited-gold-nanoparticles-characterization-and-application-in-human-saliva
#3
Paolo Bollella, Lo Gorton, Roland Ludwig, Riccarda Antiochia
Efficient direct electron transfer (DET) between a cellobiose dehydrogenase mutant from Corynascus thermophilus (CtCDH C291Y) and a novel glassy carbon (GC)-modified electrode, obtained by direct electrodeposition of gold nanoparticles (AuNPs) was realized. The electrode was further modified with a mixed self-assembled monolayer of 4-aminothiophenol (4-APh) and 4-mercaptobenzoic acid (4-MBA), by using glutaraldehyde (GA) as cross-linking agent. The CtCDH C291Y/GA/4-APh,4-MBA/AuNPs/GC platform showed an apparent heterogeneous electron transfer rate constant (ks) of 19...
August 18, 2017: Sensors
https://www.readbyqxmd.com/read/28819973/mobile-platform-for-multiplexed-detection-and-differentiation-of-disease-specific-nucleic-acid-sequences-using-microfluidic-loop-mediated-isothermal-amplification-and-smartphone-detection
#4
Weili Chen, Hojeong Yu, Fu Sun, Akid Ornob, Ryan Brisbin, Anurup Ganguli, Vinay Kumar Vemuri, Piotr Strzebonski, Guangzhe Cui, Karen J Allen, Smit A Desai, Weiran Lin, David M Nash, David Hirschberg, Ian Brooks, Rashid Bashir, Brian T Cunningham
New tools are needed to enable rapid detection, identification, and reporting of infectious viral and microbial pathogens in a wide variety of point-of-care applications that impact human and animal health. We report the design, construction, and characterization of a platform for multiplexed analysis of disease-specific DNA sequences that utilizes a smartphone camera as the sensor in conjunction with a handheld "cradle" that interfaces the phone with a silicon-based microfluidic chip embedded within a credit card-sized cartridge...
August 18, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28819666/ph-responsive-prodrug-nanoparticles-based-on-a-sodium-alginate-derivative-for-selective-co-release-of-doxorubicin-and-curcumin-into-tumor-cells
#5
Cheng Gao, Fan Tang, Guiyi Gong, Jianxiang Zhang, Maggie P M Hoi, Simon M Y Lee, Ruibing Wang
In order to realize a combination of chemotherapy and selective drug release into tumor cells, novel pH-sensitive prodrugnanoparticles were designed and prepared via the self-assembly of a synthetic amphiphilic macromolecular prodrug for the selective co-delivery of doxorubicin (Dox) and curcumin (Cur). Dox was covalently conjugated to the oxidized sodium alginate through a Schiff base reaction to produce an amphiphilic macromolecular prodrug, and the prodrug was subsequently self-assembled into nanoparticles (Dox-NPs) in an aqueous solution, which were responsive to the acidic environment in tumor cells...
August 18, 2017: Nanoscale
https://www.readbyqxmd.com/read/28818713/historical-human-exposure-to-perfluoroalkyl-acids-in-the-united-states-and-australia-reconstructed-from-biomonitoring-data-using-population-based-pharmacokinetic-modelling
#6
Melissa I Gomis, Robin Vestergren, Matthew MacLeod, Jochen F Mueller, Ian T Cousins
Perfluorooctanoic acid (PFOA), perfluorooctanesulfonic acid (PFOS) and perfluorohexanesulfonic acid (PFHxS) are found in the blood of humans and wildlife worldwide. Since the beginning of the 21st century, a downward trend in the human body burden, especially for PFOS and PFOA, has been observed while there is no clear temporal trend in wildlife. The inconsistency between the concentration decline in human serum and in wildlife could be indicative of a historical exposure pathway for humans linked to consumer products that has been reduced or eliminated...
August 14, 2017: Environment International
https://www.readbyqxmd.com/read/28817679/increased-half-life-and-enhanced-potency-of-fc-modified-human-pcsk9-monoclonal-antibodies-in-primates
#7
Yijun Shen, Hua Li, Li Zhao, Gang Li, Ben Chen, Qingsong Guo, Bei Gao, Jinsong Wu, Tong Yang, Li Jin, Yong Su
Blocking proprotein convertase subtilisin kexin type 9 (PCSK9) binding to low-density lipoprotein receptor (LDLR) can profoundly lower plasma LDL levels. Two anti-PCKS9 monoclonal antibodies (mAbs), alirocumab and evolocumab, were approved by the FDA in 2015. The recommended dose is 75 mg to 150 mg every two weeks for alirocumab and 140mg every two weeks or 420 mg once a month for evolocumab. This study attempted to improve the pharmacokinetic properties of F0016A, an IgG1 anti-PCKS9 mAb, to generate biologically superior molecules...
2017: PloS One
https://www.readbyqxmd.com/read/28817670/synthesis-radiosynthesis-and-in-vitro-evaluation-of-18f-bodipy-c16-triglyceride-as-a-dual-modal-imaging-agent-for-brown-adipose-tissue
#8
Andreas Paulus, Marco Maenen, Natascha Drude, Emmani B M Nascimento, Wouter D van Marken Lichtenbelt, Felix M Mottaghy, Matthias Bauwens
BACKGROUND: Brown adipose tissue research is in the focus in the field of endocrinology. We designed a dual-modal fluorescent/PET fatty acid based tracer on commercially available Bodipy-C16, which can be synthesized to its corresponding triglyceride and which combines the benefits of fluorescent and PET imaging. METHODS: Bodipy-C16 was coupled to 1,3-diolein resulting in Bodipy-triglyceride. Bodipy-C16 and Bodipy-triglyceride compounds were radiolabeled with 18F using an 18F/19F exchange reaction to yield a dual-modal imaging molecule...
2017: PloS One
https://www.readbyqxmd.com/read/28817135/using-microct-to-assess-periodontal-regeneration-outcomes-comparison-of-image-based-and-histologic-results-a-case-report
#9
Alberto Rebaudi, Paolo Trisi, Giorgio Pagni, Hom-Lay Wang
The purpose of this study was to compare microcomputed tomography (microCT) and histologic analysis outcomes of a periodontal regeneration of a human defect treated with a polylactic- and polyglycolic-acid copolymer. At 11 months following the grafting procedure, the root with the surrounding periodontal tissues was removed and analyzed using microCT and histologic techniques. The results suggest that microCT three-dimensional analysis may be used in synergy with two-dimensional histologic sections to provide additional information for studying the regeneration outcomes normally reported by histologic biopsies in humans...
September 2017: International Journal of Periodontics & Restorative Dentistry
https://www.readbyqxmd.com/read/28817123/evaluation-of-telomere-length-in-human-cardiac-tissues-using-cardiac-quantitative-fish
#10
Maryam Sharifi-Sanjani, Alan K Meeker, Foteini Mourkioti
Telomere length has been correlated with various diseases, including cardiovascular disease and cancer. The use of currently available telomere-length measurement techniques is often restricted by the requirement of a large amount of cells (Southern-based techniques) or the lack of information on individual cells or telomeres (PCR-based methods). Although several methods have been used to measure telomere length in tissues as a whole, the assessment of cell-type-specific telomere length provides valuable information on individual cell types...
September 2017: Nature Protocols
https://www.readbyqxmd.com/read/28817082/omega-3-long-chain-fatty-acids-in-the-heart-kidney-liver-and-plasma-metabolite-profiles-of-australian-prime-lambs-supplemented-with-pelleted-canola-and-flaxseed-oils
#11
Don V Nguyen, Van H Le, Quang V Nguyen, Bunmi S Malau-Aduli, Peter D Nichols, Aduli E O Malau-Aduli
The objective of the study was to ascertain whether human health beneficial omega-3 long-chain (≥C20) polyunsaturated fatty acid (n-3 LC-PUFA) content in heart, kidney and liver can be enhanced by supplementing prime lambs with graded levels of canola and flaxseed oil. Health status of the lambs, as a consequence of the supplementation, was also investigated by examining their plasma metabolites. Sixty purebred and first-cross lambs were allocated to one of five treatments of lucerne hay basal diet supplemented with isocaloric and isonitrogenous wheat-based pellets without oil inclusion (Control) or graded levels of canola oil at 2...
August 17, 2017: Nutrients
https://www.readbyqxmd.com/read/28816646/chronic-cigarette-smoke-mediated-global-changes-in-lung-mucoepidermoid-cells-a-phosphoproteomic-analysis
#12
Hitendra S Solanki, Jayshree Advani, Aafaque Ahmad Khan, Aneesha Radhakrishnan, Nandini A Sahasrabuddhe, Sneha M Pinto, Xiaofei Chang, Thottethodi Subrahmanya Keshava Prasad, Premendu Prakash Mathur, David Sidransky, Harsha Gowda, Aditi Chatterjee
Proteomics analysis of chronic cigarette smoke exposure is a rapidly emerging postgenomics research field. While smoking is a major cause of lung cancer, functional studies using proteomics approaches could enrich our mechanistic understanding of the elusive lung cancer global molecular signaling and cigarette smoke relationship. We report in this study on a stable isotope labeling by amino acids in cell culture-based quantitative phosphoproteomic analysis of a human lung mucoepidermoid carcinoma cell line, H292 cells, chronically exposed to cigarette smoke...
August 2017: Omics: a Journal of Integrative Biology
https://www.readbyqxmd.com/read/28816622/obtention-and-characterization-of-the-recombinant-simian-interleukin-15-in-escherichia-coli-for-the-preclinical-assessment-of-an-il-15-based-therapeutic-vaccine
#13
Yunier Rodríguez-Álvarez, Klaudia Martínez-Cordovez, Alexey Llopiz-Arzuaga, Yassel Ramos-Pérez, Vladimir Besada-Pérez, Dayana García-Lines, Alicia Santos-Savio
Recombinant simian IL-15 (siIL-15) was obtained for the preclinical assessment of an anti-human IL-15 vaccine. For this purpose, the cDNA from peripheral blood mononuclear cells of a Macaca fascicularis monkey was cloned into a pIL-2 vector. The siIL-15 was expressed in E. coli strain W3110 as an insoluble protein which accounted for 13% of the total cellular proteins. Inclusion bodies were solubilized in an 8 M urea solution, which was purified by ion exchange and reverse phase chromatography up to 92% purity...
August 17, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28816544/animal-inflammation-based-models-of-depression-and-their-application-to-drug-discovery
#14
Li Ma, Konstantin A Demin, Tatyana O Kolesnikova, Sergey L Kharsko, Xiaokang Zhu, Xiaodong Yuan, Cai Song, Darya A Meshalkina, Brian E Leonard, Li Tian, Allan V Kalueff
Depression, anxiety and other affective disorders are globally widespread and severely debilitating human brain diseases. Despite their high prevalence and mental health impact, affective pathogenesis is poorly understood, and often remains recurrent and resistant to treatment. The lack of efficient antidepressants and presently limited conceptual innovation necessitate novel approaches and new drug targets in the field of antidepressant therapy. Areas covered: Herein, the authors discuss the emerging role of neuro-immune interactions in affective pathogenesis, which can become useful targets for CNS drug discovery, including modulating neuroinflammatory pathways to alleviate affective pathogenesis...
August 17, 2017: Expert Opinion on Drug Discovery
https://www.readbyqxmd.com/read/28816439/1-3-dipolar-cycloaddition-reactions-for-the-synthesis-of-novel-oxindole-derivatives-and-their-cytotoxic-properties
#15
Prakash Rambhau Mali, Prashishkumar Kisan Shirsat, Navnath Bapurao Khomane, V Lakshama Nayak, Jagadeesh Babu Nanubolu, Harshadas Mitaram Meshram
The multicomponent reaction between isatin, amino acid, but-2-ynedioates and phenacyl bromide has been developed using microwave irradiation under catalyst and base free conditions in aqueous medium. This synthetic protocol is useful for the synthesis of various functionalized spirooxindole derivatives. This MCR exhibits a broad substrate scope with excellent yields and shorter reaction time. Additionally the synthesized spirooxindole derivatives were evaluated for their anticancer activity against three human cancer cell lines; MCF-7 (breast), A549 (lung) and Hela cervical...
August 17, 2017: ACS Combinatorial Science
https://www.readbyqxmd.com/read/28815804/octadentate-picolinic-acid-based-bispidine-ligand-for-radiometal-ions
#16
Peter Comba, Una Jermilova, Chris Orvig, Brian O Patrick, Caterina F Ramogida, Katharina Rück, Christina Schneider, Miriam Starke
The synthesis of the octadentate bispidine ligand H2bispa2 bearing two picolinic acid pendant arms and its coordination chemistry with metal ions with radionuclides relevant for nuclear medicine, i.e. indium(III) (111In), lutetium(III) (177Lu) and lanthanum(III) (as surrogate for 225Ac), are reported. The non-radioactive metal complexes of the N6O2-type bispa ligand were characterized by 1H and 13C nuclear magnetic resonance spectroscopy, elemental analysis, mass spectrometry and single crystal X-ray analysis...
August 16, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28815576/microrna-and-mesial-temporal-lobe-epilepsy-with-hippocampal-sclerosis-whole-mirnome-profiling-of-human-hippocampus
#17
Petra Bencurova, Jiri Baloun, Katerina Musilova, Lenka Radova, Boris Tichy, Martin Pail, Martin Zeman, Eva Brichtova, Marketa Hermanova, Sarka Pospisilova, Marek Mraz, Milan Brazdil
OBJECTIVE: Mesial temporal lobe epilepsy (mTLE) is a severe neurological disorder characterized by recurrent seizures. mTLE is frequently accompanied by neurodegeneration in the hippocampus resulting in hippocampal sclerosis (HS), the most common morphological correlate of drug resistance in mTLE patients. Incomplete knowledge of pathological changes in mTLE+HS complicates its therapy. The pathological mechanism underlying mTLE+HS may involve abnormal gene expression regulation, including posttranscriptional networks involving microRNAs (miRNAs)...
August 16, 2017: Epilepsia
https://www.readbyqxmd.com/read/28815284/analysis-of-lipophilic-marine-biotoxins-by-liquid-chromatography-coupled-with-high-resolution-mass-spectrometry-in-seawater-from-the-catalan-coast
#18
Cristina Bosch-Orea, Josep Sanchís, Marinella Farré, Damià Barceló
Marine biotoxins regularly occur along the coast, with several consequences for the environment as well as the food industry. Monitoring of these compounds in seawater is required to assure the safety of marine resources for human consumption, providing a means for forecasting shellfish contamination events. In this study, an analytical method was developed for the detection of ten lipophilic marine biotoxins in seawater: azaspiracids 1, 2, 3, 4 and 5, classified as azaspiracid shellfish poisoning toxins, and pectenotoxin 2, okadaic acid and the related dinophysistoxin 1, yessotoxin and homoyessotoxin, classified as diarrheic shellfish poisoning toxins...
August 16, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28814525/structure-guided-functional-annotation-of-the-influenza-a-virus-ns1-protein-reveals-dynamic-evolution-of-the-p85%C3%AE-binding-site-during-circulation-in-humans
#19
Antonio M Lopes, Patricia Domingues, Roland Zell, Benjamin G Hale
Rational characterization of virulence and host-adaptive markers in the multifunctional influenza A virus NS1 protein is hindered by a lack of comprehensive knowledge about NS1-host protein-protein interfaces. Here, we surveyed the impact of amino-acid variation in NS1 at its structurally-defined binding site for host p85β, a regulator of phosphoinositide-3 kinase (PI3K) signaling. Structure-guided alanine-scanning of all viral residues at this interface defined 10 positions contributing to the interaction, with residues 89, 95, 98, 133, 145 and 162 being most important...
August 16, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28814238/structural-insights-for-drugs-developed-for-phospholipase-d-enzymes
#20
Kimberly Stieglitz
BACKGROUND: In recent years human phospholipase D enzymes (PLD1 and PLD2 isozymes) have emerged as drug targets for various diseases such as cardiovascular disease, cancer, infectious diseases and neurodegenerative conditions such as Alzheimer's and Parkinson's disease. The interest in PLD as a drug target is due to the fact that PLD enzymes belong to a superfamily of phospholipases that are essential to intracellular and extracellular signaling. Many bioactive lipid signaling molecules are generated by these enzymes including phosphatidic and lysophosphatidic acid, arachidonic acid, and diacylglycerol (DAG)...
August 15, 2017: Current Drug Discovery Technologies
keyword
keyword
21382
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"