keyword
MENU ▼
Read by QxMD icon Read
search

ACTR2

keyword
https://www.readbyqxmd.com/read/27791462/deregulation-of-focal-adhesion-formation-and-cytoskeletal-tension-due-to-loss-of-a-type-lamins
#1
Tobias D J Corne, Tom Sieprath, Jonathan Vandenbussche, Danahe Mohammed, Mariska Te Lindert, Kris Gevaert, Sylvain Gabriele, Katarina Wolf, Winnok H De Vos
The nuclear lamina mechanically integrates the nucleus with the cytoskeleton and extracellular environment and regulates gene expression. These functions are exerted through direct and indirect interactions with the lamina's major constituent proteins, the A-type lamins, which are encoded by the LMNA gene. Using quantitative stable isotope labeling-based shotgun proteomics we have analyzed the proteome of human dermal fibroblasts in which we have depleted A-type lamins by means of a sustained siRNA-mediated LMNA knockdown...
October 28, 2016: Cell Adhesion & Migration
https://www.readbyqxmd.com/read/27559607/protein-interaction-network-of-human-protein-kinase-d2-revealed-by-chemical-cross-linking-mass-spectrometry
#2
Björn Häupl, Christian H Ihling, Andrea Sinz
We investigated the interaction network of human PKD2 in the cytosol and in Golgi-enriched subcellular protein fractions by an affinity enrichment strategy combined with chemical cross-linking/mass spectrometry (MS). Analysis of the subproteomes revealed the presence of distinct proteins in the cytosolic and Golgi fractions. The covalent fixation of transient or weak interactors by chemical cross-linking allowed capturing interaction partners that might otherwise disappear during conventional pull-down experiments...
October 7, 2016: Journal of Proteome Research
https://www.readbyqxmd.com/read/27184932/high-expression-of-integrin-%C3%AE-6-in-association-with-the-rho-rac-pathway-identifies-a-poor-prognostic-subgroup-within-her2-amplified-breast-cancers
#3
Krisha Desai, Madhumathy G Nair, Jyothi S Prabhu, Anupama Vinod, Aruna Korlimarla, Savitha Rajarajan, Radhika Aiyappa, Rohini S Kaluve, Annie Alexander, P S Hari, Geetashree Mukherjee, Rekha V Kumar, Suraj Manjunath, Marjorrie Correa, B S Srinath, Shekhar Patil, M S N Prasad, K S Gopinath, Raman N Rao, Shelia M Violette, Paul H Weinreb, T S Sridhar
Integrin αvβ6 is involved in the transition from ductal carcinoma in situ (DCIS) to invasive ductal carcinoma (IDC) of the breast. In addition, integrin β6 (ITGB6) is of prognostic value in invasive breast cancers, particularly in HER2+ subtype. However, pathways mediating the activity of integrin αvβ6 in clinical progression of invasive breast cancers need further elucidation. We have examined human breast cancer specimens (N = 460) for the expression of integrin β6 (ITGB6) mRNA by qPCR. In addition, we have examined a subset (N = 147) for the expression of αvβ6 integrin by immunohistochemistry (IHC)...
August 2016: Cancer Medicine
https://www.readbyqxmd.com/read/25585696/genome-wide-analysis-identifies-a-role-for-common-copy-number-variants-in-specific-language-impairment
#4
Nuala H Simpson, Fabiola Ceroni, Rose H Reader, Laura E Covill, Julian C Knight, Elizabeth R Hennessy, Patrick F Bolton, Gina Conti-Ramsden, Anne O'Hare, Gillian Baird, Simon E Fisher, Dianne F Newbury
An exploratory genome-wide copy number variant (CNV) study was performed in 127 independent cases with specific language impairment (SLI), their first-degree relatives (385 individuals) and 269 population controls. Language-impaired cases showed an increased CNV burden in terms of the average number of events (11.28 vs 10.01, empirical P=0.003), the total length of CNVs (717 vs 513 Kb, empirical P=0.0001), the average CNV size (63.75 vs 51.6 Kb, empirical P=0.0005) and the number of genes spanned (14.29 vs 10...
October 2015: European Journal of Human Genetics: EJHG
https://www.readbyqxmd.com/read/24996170/modeling-a-genetic-risk-for-schizophrenia-in-ipscs-and-mice-reveals-neural-stem-cell-deficits-associated-with-adherens-junctions-and-polarity
#5
Ki-Jun Yoon, Ha Nam Nguyen, Gianluca Ursini, Fengyu Zhang, Nam-Shik Kim, Zhexing Wen, Georgia Makri, David Nauen, Joo Heon Shin, Youngbin Park, Raeeun Chung, Eva Pekle, Ce Zhang, Maxwell Towe, Syed Mohammed Qasim Hussaini, Yohan Lee, Dan Rujescu, David St Clair, Joel E Kleinman, Thomas M Hyde, Gregory Krauss, Kimberly M Christian, Judith L Rapoport, Daniel R Weinberger, Hongjun Song, Guo-Li Ming
Defects in brain development are believed to contribute toward the onset of neuropsychiatric disorders, but identifying specific underlying mechanisms has proven difficult. Here, we took a multifaceted approach to investigate why 15q11.2 copy number variants are prominent risk factors for schizophrenia and autism. First, we show that human iPSC-derived neural progenitors carrying 15q11.2 microdeletion exhibit deficits in adherens junctions and apical polarity. This results from haploinsufficiency of CYFIP1, a gene within 15q11...
July 3, 2014: Cell Stem Cell
https://www.readbyqxmd.com/read/22935087/tandem-duplication-of-kit-exon-11-influences-the-proteome-of-canine-mast-cell-tumours
#6
P Schlieben, A Meyer, C Weise, A Bondzio, A D Gruber, R Klopfleisch
Mutations with permanent activation of the stem cell factor receptor KIT have been identified as one potential cause for canine cutaneous mast cell tumours (MCTs). The exact changes in global gene expression patterns associated with permanent activation of KIT in these tumours are unknown. The present study compares, by the use of two dimensional difference gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, the proteomes of canine MCTs, with and without KIT exon 11 tandem duplication...
May 2013: Journal of Comparative Pathology
https://www.readbyqxmd.com/read/22876139/screening-of-candidate-genes-for-primary-open-angle-glaucoma
#7
Ting Liu, Lin Xie, Jian Ye, Yuewuyang Liu, Xiangge He
PURPOSE: Primary open-angle glaucoma (POAG) is one of the leading causes of irreversible blindness in the world. To make progress in understanding POAG, it is necessary to identify more POAG-causing genes. METHODS: Using haplotype analysis, we found that mutational region is located on chromosome 2 in two families. Furthermore, we screened 11 candidate genes on chromosome 2 by protein-protein interaction (PPI) analysis, including mutS homolog 6 (MSH6), mutS homolog 2 (MSH2), v-rel reticuloendotheliosis viral oncogene homolog (REL), endothelial PAS domain protein 1 (EPAS1), vaccinia related kinase 2 (VRK2), F-box protein 11 (FBXO11), EGF containing fibulin-like extracellular matrix protein 1 (EFEMP1), reticulon 4 (RTN4), RAB1A, member RAS oncogene family (RAB1A), ARP2 actin-related protein 2 homolog (ACTR2), and calmodulin 2 (phosphorylase kinase, delta; CALM2)...
2012: Molecular Vision
https://www.readbyqxmd.com/read/8307945/activin-a-binds-to-a-heterotrimeric-receptor-complex-on-the-vascular-endothelial-cell-surface-evidence-for-a-type-3-activin-receptor
#8
S A McCarthy, R Bicknell
The effect of transfection of the type 2 activin receptor, ACTR2, on binding of 125I-activin-A to the surface of bovine aortic endothelial cells (BAEC) was investigated. BAEC transfected either with full-length ACTR2 or with a truncated form of ACTR2 lacking the intracellular kinase domain (ACTR2T) displayed two classes of 125I-activin-A binding sites, one of high affinity (Kd = 250-254 pM) and one of low affinity (Kd = 6.5-16 nM). Affinity labeling of ACTR2-transfected BAEC with 125I-activin-A revealed labeled species of 55, 95, 100, and 160 kDa, all four of which were immunoprecipitated by an anti-ACTR2 monoclonal antibody...
February 11, 1994: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/7927363/monoclonal-antibodies-that-recognize-the-type-2-activin-receptor-actr2
#9
S A McCarthy, H Turley, K C Gatter, R Bicknell
Monoclonal antibodies (MAbs) were raised in mice against a bacterial fusion protein composed of the intracellular serine/threonine kinase domain of the type-2 activin receptor, ACTR2, fused to glutathione S-transferase. Three MAbs with high affinity toward the ACTR2 kinase domain were isolated, one of which recognized specifically ACTR2 expressed transiently in vascular endothelial cells. These reagents should be of use in the elucidation of mechanisms of transmembrane signaling by this member of the emerging receptor serine threonine kinase family...
June 1994: Hybridoma
1
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"