keyword
https://read.qxmd.com/read/36142793/different-approaches-for-the-profiling-of-cancer-pathway-related-genes-in-glioblastoma-cells
#21
JOURNAL ARTICLE
Zuzana Majercikova, Katarina Dibdiakova, Michal Gala, Denis Horvath, Radovan Murin, Gabriel Zoldak, Jozef Hatok
Deregulation of signalling pathways that regulate cell growth, survival, metabolism, and migration can frequently lead to the progression of cancer. Brain tumours are a large group of malignancies characterised by inter- and intratumoral heterogeneity, with glioblastoma (GBM) being the most aggressive and fatal. The present study aimed to characterise the expression of cancer pathway-related genes ( n = 84) in glial tumour cell lines (A172, SW1088, and T98G). The transcriptomic data obtained by the qRT-PCR method were compared to different control groups, and the most appropriate control for subsequent interpretation of the obtained results was chosen...
September 17, 2022: International Journal of Molecular Sciences
https://read.qxmd.com/read/35966301/autophagy-related-dna-methylation-signature-predict-clinical-prognosis-and-immune-microenvironment-in-low-grade-glioma
#22
JOURNAL ARTICLE
Qiujiang Qiao, Yanjun Wang, Rui Zhang, Qi Pang
Background: Low-grade glioma (LGG) is a common malignant tumor of the central nervous system. The clinical prognosis of different patients varies greatly, so exploring appropriate markers that affect the prognosis and treatment of LGG is important. The purpose of this study was to identify the potential effect of autophagy-related DNA methylation on the prognosis and immune microenvironment in LGG. Methods: The methylation profile, transcription data and corresponding clinical information of 451 patients with LGG were obtained from The Cancer Genome Atlas (TCGA)...
July 2022: Translational Cancer Research
https://read.qxmd.com/read/35954468/elevated-mir-29c-5p-expression-in-nipple-aspirate-fluid-is-associated-with-extremely-high-mammographic-breast-density
#23
JOURNAL ARTICLE
Tessa A C M Vissers, Leonie Piek, Susana I S Patuleia, Aafke J Duinmeijer, Marije F Bakker, Elsken van der Wall, Paul J van Diest, Carla H van Gils, Cathy B Moelans
High mammographic density (MD) is associated with an increased risk of breast cancer, however the underlying mechanisms are largely unknown. This research aimed to identify microRNAs (miRNAs) that play a role in the development of extremely dense breast tissue. In the discovery phase, 754 human mature miRNAs were profiled in 21 extremely high MD- and 20 very low MD-derived nipple aspirate fluid (NAF) samples from healthy women. In the validation phase, candidate miRNAs were assessed in a cohort of 89 extremely high MD and 81 very low MD NAF samples from healthy women...
August 5, 2022: Cancers
https://read.qxmd.com/read/35940633/duck-cd8-t-cell-response-to-h5n1-highly-pathogenic-avian-influenza-virus-infection-in-vivo-and-in-vitro
#24
JOURNAL ARTICLE
Manman Dai, Hui Sun, Li Zhao, Qingxin Wu, Bowen You, Fengxiang Xu, Jiayu Liao, Sufang Zhu, Ziwei Li, Yongxiu Yao, Venugopal Nair, Ming Liao
Domestic ducks are the important host for H5N1 highly pathogenic avian influenza virus (HPAIV) infection and epidemiology, but little is known about the duck T cell response to H5N1 AIV infection. In infection experiments of mallard ducks, we detected significantly increased CD8+ cells and augmented expression of cytotoxicity-associated genes, including granzyme A and IFN-γ, in PBMCs from 5 to 9 d postinfection when the virus shedding was clearly decreased, which suggested the importance of the duck cytotoxic T cell response in eliminating H5N1 infection in vivo...
August 8, 2022: Journal of Immunology
https://read.qxmd.com/read/35923699/prognostic-model-and-nomogram-construction-and-validation-with-an-autophagy-related-gene-signature-in-low-grade-gliomas
#25
JOURNAL ARTICLE
Xinrui Li, Zhiyuan Huang, Lei Zhu, Fei Yu, Minghao Feng, Aiqin Gu, Jianxin Jiang, Guangxue Wang, Dongya Huang
Background : Autophagy plays a vital role in cancer development. However, the prognostic value of autophagy-related genes (ARGs) in low-grade gliomas (LGG) is unclear. This research aimed to investigate whether ARGs correlated with overall survival (OS) in LGG patients. Methods: RNA-sequencing data were obtained from The Cancer Genome Atlas (TCGA) TARGET GTEx database. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis of ARGs were performed by the "clusterprofile" R package. Cox regression with the wald χ2 test was employed to identify prognostic significant ARGs...
2022: Frontiers in Genetics
https://read.qxmd.com/read/35891784/crystallographic-mining-of-ask1-regulators-to-unravel-the-intricate-ppi-interfaces-for-the-discovery-of-small-molecule
#26
REVIEW
Ashish Kumar Agrahari, Madhu Dikshit, Shailendra Asthana
Protein seldom performs biological activities in isolation. Understanding the protein-protein interactions' physical rewiring in response to pathological conditions or pathogen infection can help advance our comprehension of disease etiology, progression, and pathogenesis, which allow us to explore the alternate route to control the regulation of key target interactions, timely and effectively. Nonalcoholic steatohepatitis (NASH) is now a global public health problem exacerbated due to the lack of appropriate treatments...
2022: Computational and Structural Biotechnology Journal
https://read.qxmd.com/read/35819261/pharmacological-targeting-of-tfiih-suppresses-kras-mutant-pancreatic-ductal-adenocarcinoma-and-synergizes-with-trail
#27
JOURNAL ARTICLE
Russell Moser, James Annis, Olga Nikolova, Clifford J Whatcott, Kay E Gurley, Eduardo Mendez, Kim Moran-Jones, Craig Dorrell, Rosalie C Sears, Calvin J Kuo, Haiyong Han, Andrew V Biankin, Carla Grandori, Daniel D Von Hoff, Christopher J Kemp
Pancreatic ductal adenocarcinoma (PDAC) typically presents as metastatic disease at diagnosis and remains refractory to treatment. Next generation sequencing efforts have described the genomic landscape, classified molecular subtypes, and confirmed frequent alterations in major driver genes, with coexistent alterations in KRAS and TP53 correlating with the highest metastatic burden and poorest outcomes. However, translating this information to guide therapy remains a challenge. By integrating genomic analysis with an arrayed RNAi druggable genome screen and drug profiling of a KRAS/TP53 mutant PDAC cell line derived from a patient-derived xenograft (PDCL), we identified numerous targetable vulnerabilities that reveal both known and novel functional aspects of pancreatic cancer biology...
July 12, 2022: Cancer Research
https://read.qxmd.com/read/35546566/rps27-selectively-regulates-the-expression-and-alternative-splicing-of-inflammatory-and-immune-response-genes-in-thyroid-cancer-cells
#28
JOURNAL ARTICLE
Jing Wan, Juan Lv, Chun Wang, Li Zhang
BACKGROUND: The expression of ribosomal protein S27 (RPS27) is upregulated in multiple human malignancies. In thyroid cancer, the expression of RPS27 is associated with patient outcomes. However, the carcinogenic mechanisms of RPS27 and functions of RPS27 in the initiation and progression of thyroid cancer are still not clear. OBJECTIVES: To investigate the carcinogenic mechanisms of RPS27 and functions of RPS27 in the initiation and progression of thyroid cancer...
May 12, 2022: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
https://read.qxmd.com/read/35449571/the-prognostic-signature-of-head-and-neck-squamous-cell-carcinoma-constructed-by-immune-related-rna-binding-proteins
#29
JOURNAL ARTICLE
Ruijie Ming, Xiangrui Li, Enhao Wang, Jiahui Wei, Bo Liu, Peng Zhou, Wenting Yu, Shimin Zong, Hongjun Xiao
Purpose: This study aimed to construct a prognostic signature consisting of immune-related RNA-binding proteins (RBPs) to predict the prognosis of patients with head and neck squamous cell carcinoma (HNSCC) effectively. Methods: The transcriptome and clinical data of HNSCC were downloaded from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. First, we ascertained the immunological differences in HNSCC, through single-sample gene set enrichment analysis, stromal and immune cells in malignant tumor tissues using expression data (ESTIMATE), and cell-type identification by estimating relative subsets of RNA transcripts (CIBERSORT) deconvolution algorithm...
2022: Frontiers in Oncology
https://read.qxmd.com/read/34828017/immunomodulatory-effects-of-herbal-compounds-quercetin-and-curcumin-on-cellular-and-molecular-functions-of-bovine-milk-isolated-neutrophils-toward-streptococcus-agalactiae-infection
#30
JOURNAL ARTICLE
Purichaya Disbanchong, Wichayaporn Punmanee, Anyaphat Srithanasuwan, Noppason Pangprasit, Kanruethai Wongsawan, Witaya Suriyasathaporn, Phongsakorn Chuammitri
Herbal phytochemicals featuring active ingredients including quercetin and curcumin have shown potential in treating human and animal diseases. The current study investigated their potential function in vitro for host immunomodulation associated with Streptococcus agalactiae subclinical bovine mastitis via milk-isolated neutrophils. Our results showed a positive influence on cellular migration, reactive oxygen species (ROS) generation, phagocytosis, and bacterial killing as well as neutrophil extracellular traps (NETs) release...
November 17, 2021: Animals: An Open Access Journal From MDPI
https://read.qxmd.com/read/34737609/a-five-autophagy-related-long-non-coding-rna-prognostic-model-for-patients-with-lung-adenocarcinoma
#31
JOURNAL ARTICLE
Boxuan Liu, Shuanying Yang
Purpose: Lung adenocarcinoma is the most common pathological type among non-small cell lung cancer. Although huge progress has been made in terms of early diagnosis and precision treatment in recent years, the overall 5-year survival rate of a patient remains low. In our study, we try to construct an autophagy-related lncRNA prognostic signature that may guide clinical practice. Methods: The mRNA and lncRNA expression matrix of lung adenocarcinoma patients were retrieved from the TCGA database...
2021: International Journal of General Medicine
https://read.qxmd.com/read/34734480/exercise-ameliorates-insulin-resistance-and-improves-ask1-mediated-insulin-signalling-in-obese-rats
#32
JOURNAL ARTICLE
Yong Zhang, Tingting Ye, Puqing Zhou, Runjing Li, Zuofeng Liu, Jianyuan Xie, Tianmiao Hua, Qingyan Sun
Increasing evidence reveals that physical exercise is an efficient therapeutical approach in the treatment of insulin resistance (IR) and related metabolic diseases. However, the potential beneficial effects of exercise on insulin resistance and its underlying mechanisms remain unclear. Recent findings elucidated the negative role of ASK1 in repressing the glucose uptake through JNK1-IRS1-Akt signalling in liver. Thus, a detailed investigation of the effect of ASK1-mediated insulin signalling on exercise-mediated improvement of insulin sensitivity and its underlying mechanism was implemented in this study...
November 3, 2021: Journal of Cellular and Molecular Medicine
https://read.qxmd.com/read/34693625/bclaf1-regulates-c-flip-expression-and-protects-cells-from-tnf-induced-apoptosis-and-tissue-injury
#33
JOURNAL ARTICLE
Rui Zhang, Teng Xue, Anwen Shao, Yue Lang, Chao Qin, Mingliang Zhao, Yu Kuang, Zhengquan Yu, Yunyun Geng, Chenyang Zhao, Jun Tang
TNF stimulation generates pro-survival signals through activation of NF-κB that restrict the build-in death signaling triggered by TNF. The competition between TNF-induced survival and death signals ultimately determines the fate of a cell. Here, we report the identification of Bclaf1 as a novel component of the anti-apoptotic program of TNF. Bclaf1 depletion in multiple cells sensitizes cells to TNF-induced apoptosis but not to necroptosis. Bclaf1 exerts its anti-apoptotic function by promoting the transcription of CFLAR, a caspase 8 antagonist, downstream of NF-κB activation...
October 25, 2021: EMBO Reports
https://read.qxmd.com/read/34602774/molecular-docking-analysis-of-flupenthixol-and-desmethylastemizole-with-the-apoptotic-regulator-proteins-cflar-and-traf2-linked-to-lung-carcinoma
#34
JOURNAL ARTICLE
Subrata Das, Anupam Das Talukdar, Rajat Nath, Deepa Nath, Ashikur Rahaman, Shamee Bhattacharjee, Manbendra Dutta Choudhury
It is known that molecular changes in apoptotic genes due to mutation may cause disruption of apoptotic pathway resulting in an abrupt increase in cell proliferation. Therefore, it is of interest to identify compounds that could potentially replenish the changes in the apoptotic pathway, resulted from mutation. The gene network analysis using the Network Analyzer Plugin of Cytoscape (3.5.1) shows CFLAR and TRAF2 as influential genes in the apoptotic pathway. Mutation in these genes brings loss in apoptotic property of a cell and thus increases the cell proliferating activity...
2021: Bioinformation
https://read.qxmd.com/read/34302634/lactucin-induces-apoptosis-through-reactive-oxygen-species-mediated-bcl-2-and-cflar-l-downregulation-in-caki-1-cells
#35
JOURNAL ARTICLE
Ji Hoon Jang, Cho-Young Park, Eon-Gi Sung, In-Hwan Song, Joo-Young Kim, Chuleui Jung, Ho-Yong Sohn, Tae-Jin Lee
BACKGROUND: Lactucin, a naturally occurring active sesquiterpene lactone, is abundantly found in chicory and romaine lettuce. A recent study reported that lactucin could induce apoptosis in leukemia cells. However, its cytotoxicity and potential molecular mechanisms underlying cancer cell death remain unclear. OBJECTIVE: Therefore, in this study, we aimed to investigate the direct effect and underlying mechanism of action of lactucin on renal cancer cells. METHODS: MTT assay and flow cytometry were performed to evaluate the rate of cell proliferation and apoptosis, respectively...
July 24, 2021: Genes & Genomics
https://read.qxmd.com/read/34225733/bioinformatics-integrated-analysis-to-investigate-candidate-biomarkers-and-associated-metabolites-in-osteosarcoma
#36
JOURNAL ARTICLE
Jun Wang, Mingzhi Gong, Zhenggang Xiong, Yangyang Zhao, Deguo Xing
BACKGROUND: This study hoped to explore the potential biomarkers and associated metabolites during osteosarcoma (OS) progression based on bioinformatics integrated analysis. METHODS: Gene expression profiles of GSE28424, including 19 human OS cell lines (OS group) and 4 human normal long bone tissue samples (control group), were downloaded. The differentially expressed genes (DEGs) in OS vs. control were investigated. The enrichment investigation was performed based on DEGs, followed by protein-protein interaction network analysis...
July 5, 2021: Journal of Orthopaedic Surgery and Research
https://read.qxmd.com/read/34112763/necroptosis-protects-against-exacerbation-of-acute-pancreatitis
#37
JOURNAL ARTICLE
Michittra Boonchan, Hideki Arimochi, Kunihiro Otsuka, Tomoko Kobayashi, Hisanori Uehara, Thiranut Jaroonwitchawan, Yuki Sasaki, Shin-Ichi Tsukumo, Koji Yasutomo
The sensing of various extrinsic stimuli triggers the receptor-interacting protein kinase-3 (RIPK3)-mediated signaling pathway, which leads to mixed-lineage kinase-like (MLKL) phosphorylation followed by necroptosis. Although necroptosis is a form of cell death and is involved in inflammatory conditions, the roles of necroptosis in acute pancreatitis (AP) remain unclear. In the current study, we administered caerulein to Ripk3- or Mlkl-deficient mice (Ripk3-/- or Mlkl-/- mice, respectively) and assessed the roles of necroptosis in AP...
June 10, 2021: Cell Death & Disease
https://read.qxmd.com/read/34093817/an-integrative-microenvironment-approach-for-laryngeal-carcinoma-the-role-of-immune-methylation-autophagy-signatures-on-disease-clinical-prognosis-and-single-cell-genotypes
#38
JOURNAL ARTICLE
Xueran Kang, Yisheng Chen, Bin Yi, Xiaojun Yan, Chenyan Jiang, Bin Chen, Lixing Lu, Yuxing Sun, Runjie Shi
The effects of methylation/autophagy-related genes (MARGs) and immune infiltration in the tumor microenvironment on the prognosis of laryngeal cancer were comprehensively explored in this study. Survival analysis screened out 126 MARGs and 10 immune cells potentially associated with the prognosis of laryngeal carcinoma. Cox and lasso regression analyses were then used to select 8 MARGs (CAPN10, DAPK2, MBTPS2, ST13, CFLAR, FADD, PEX14 and TSC2) and 2 immune cells (Eosinophil and Mast cell) to obtain the prognostic risk scoring system (pRS)...
2021: Journal of Cancer
https://read.qxmd.com/read/33931647/cut-like-homeobox-1-cux1-tumor-suppressor-gene-haploinsufficiency-induces-apoptosis-evasion-to-sustain-myeloid-leukemia
#39
JOURNAL ARTICLE
Emmanuelle Supper, Saskia Rudat, Vivek Iyer, Alastair Droop, Kim Wong, Jean-François Spinella, Patrick Thomas, Guy Sauvageau, David J Adams, Chi C Wong
While oncogenes promote tumorigenesis, they also induce deleterious cellular stresses, such as apoptosis, that cancer cells must combat by coopting adaptive responses. Whether tumor suppressor gene haploinsufficiency leads to such phenomena and their mechanistic basis is unclear. Here, we demonstrate that elevated levels of the anti-apoptotic factor, CASP8 and FADD-like apoptosis regulator (CFLAR), promotes apoptosis evasion in acute myeloid leukemia (AML) cells haploinsufficient for the cut-like homeobox 1 (CUX1) transcription factor, whose loss is associated with dismal clinical prognosis...
April 30, 2021: Nature Communications
https://read.qxmd.com/read/33769609/cdkn2a-deregulation-in-fatty-liver-disease-and-its-accelerative-role-in-the-process-of-lipogenesis
#40
JOURNAL ARTICLE
Zhi Zhang, Huiqing Wen, Bangjian Peng, Jun Weng, Fanhong Zeng
Previous literature has indicated that cyclin-dependent kinase inhibitor 2 A (CDKN2A) is upregulated, while the Protein Inhibitor of Activated STAT1 (PIAS1) is downregulated in the liver tissues of obese mice. The current study aimed to investigate the relationship between CDKN2A and PIAS1 in the lipogenesis of fatty liver disease. In the C57BL/6J db/db mouse model and hepatocyte model of fatty liver, the expression pattern of CDKN2A, PIAS1, Protein arginine methyltransferase 1 (PRMT1) and CASP8 and FADD-like apoptosis regulator (CFLAR) was characterized by RNA quantitative and Western blot analysis...
April 2021: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
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