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Hongxia Yang, Gang Zhang, Xiaolin Che, Shudong Yu
Slug is associated with the radioresistance of nasopharyngeal carcinoma (NPC) and the main current approach of treatment for NPC is radiotherapy. Hence, the aim of the current study was to determine the effect of Slug silencing on the radiosensitivity of NPC cells. Lentiviral-mediated transfection of Slug RNA interference (RNAi) in NPC cell line C666-1 was performed in vitro . Following Slug inhibition, its expression was detected using western blotting. A clonogenic survival assay and flow cytometry were then performed to evaluate the clonogenic cell survival, cell cycle distribution and apoptosis of C666-1 cells following irradiation...
April 2018: Experimental and Therapeutic Medicine
Yumao Jiang, Yue Jiao, Yang Liu, Meiyu Zhang, Zhiguo Wang, Yujuan Li, Tao Li, Xiaoliang Zhao, Danqiao Wang
As shown in our previous study, sinomenine hydrochloride (SH), the major bioactive alkaloid isolated from Sinomenium acutum Rehd. et Wils. (Fam. Menispermaceae ), initiates the autophagy-mediated death of human glioblastoma cells by generating reactive oxygen species and activating the autophagy-lysosome pathway. However, its effects on the migration and invasion of human glioblastoma cells have not yet been elucidated. Therefore, human glioblastoma U87 and SF767 cells were treated with SH (0.125 and 0.25 mM) for 24 h, and cell migration and invasion were assessed using scratch wound healing, migration and invasion assays...
March 14, 2018: International Journal of Molecular Sciences
Tingting Hu, Mengyun Yao, Xiaoxia Fu, Chaolu Chen, Ruijin Wu
Polychlorinated biphenyls (PCBs), as part of environmental contaminants, have been proved to be related to endometriosis. This study is to investigate the effect of PCB 104 on cell migration, invasion and resultant gene expression in endometrial stromal cells (ESCs). Fifty-three specimens of eutopic endometrial tissues were collected from twenty-four women with endometriosis (EU-EMS) and twenty-nine women without endometriosis (EU-NON). Both EU-EMS and EU-NON were divided into the PCB 104 exposure group and the control group according to whether they were exposed to PCB 104...
March 10, 2018: Toxicology Letters
GuangLiang Jiang, QingFeng Hu, Hui Wang, WeiHong Ding, Ning Zhang, YiShuo Wu, QiDong Zhou, ChuanYu Sun, GuoWei Xia, Qiang Ding, Rong Na, Ke Xu
BACKGROUND: Epithelial-mesenchymal transition (EMT) was reported to have an important effect on malignant metastasis; however, it remained largely unknown if EMT marker expression of neoplastic tissue had predictive value for prognosis of prostate cancer. METHODS: We searched for published studies which measured EMT marker expression and analyzed its association with clinical outcomes of patients after Radical Prostatectomy (RP). We reviewed and pooled-analyzed the association of EMT marker expression and biochemical recurrence-free survival (BFS), as well as the difference in strong or weak expression of EMT markers in tumors of high Gleason score (≥8)...
January 2018: Annals of Clinical and Laboratory Science
Alexandre Lobo-da-Cunha, Diogo Amaral-de-Carvalho, Elsa Oliveira, Ângela Alves, Vítor Costa, Gonçalo Calado
Mannitol oxidase and polyol dehydrogenases are enzymes that convert polyalcohols into sugars. Mannitol oxidase was previously investigated in terrestrial snails and slugs, being also present in a few aquatic gastropods. However, the overall distribution of this enzyme in the Gastropoda was not known. Polyol dehydrogenases are also poorly studied in gastropods and other mollusks. In this study, polyalcohol oxidase and dehydrogenase activities were assayed in the digestive gland of 26 species of gastropods, representing the clades Patellogastropoda, Neritimorpha, Vetigastropoda, Caenogastropoda and Heterobranchia...
2018: PloS One
Gunjan Mandal, Subir Biswas, Sougata Roy Chowdhury, Annesha Chatterjee, Suman Purohit, Poulomi Khamaru, Sayan Chakraborty, Palash Kumar Mandal, Arnab Gupta, Jo-Anne de la Mare, Adrienne Lesley Edkins, Arindam Bhattacharyya
The multifunctional cytokine TGF-β crucially participates in breast cancer (BCa) metastasis and works differently in the disease stages, thus contributing in BCa progression. We address connections between TGF-β and the stem cell-related transcription factor (TF) Oct4 in BCa. In 147 BCa patients with infiltrating duct carcinoma, we identified a significantly higher number of cases with both moderate/high Oct4 expression and high TGF-β in late stages compared to early stages of the disease. In vitro studies showed that TGF-β elevated Oct4 expression, which in turn, regulated Epithelial-to-Mesenchymal transition (EMT)-regulatory gene (Snail and Slug) expression, migratory ability, chemotactic invasiveness and extracellular matrix (ECM) degradation potential of BCa cells...
March 8, 2018: Biochimica et Biophysica Acta
Raffaella Santi, Tommaso Cai, Stefania Nobili, Ilaria Camilla Galli, Andrea Amorosi, Eva Comperat, Gabriella Nesi
The current WHO/ISUP classification and grading system subdivides urothelial tumours into prognostically distinct categories. Understanding the molecular pathways involved in bladder cancer development can improve patient stratification and management. This study aims to investigate the relationship between Snail, Slug and E-cadherin expressions and clinico-pathological features of non-muscle invasive bladder carcinoma (NMIBC). All patients attending the same urological centre from January to May 2002, who were pathologically diagnosed with NMIBC, were enrolled in this longitudinal cohort study...
March 10, 2018: Virchows Archiv: An International Journal of Pathology
John S Berriman, Ryan A Ellingson, Jaymes D Awbrey, Diane M Rico, Ángel A Valdés, Nerida G Wilson, Andres Aguilar, David G Herbert, Yayoi M Hirano, Cynthia D Trowbridge, Patrick J Krug
Predicting biotic resistance to highly invasive strains of "killer algae" (Caulerpa spp.) requires understanding the diversity and feeding preferences of native consumers, including sea slugs in family Oxynoidae. Past studies reported low algal host specificity for Oxynoe (6 spp.) and Lobiger (4 spp.), but these taxonomically challenging slugs may represent species complexes of unrecognized specialists that prefer different Caulerpa spp. Here, we assess global diversity of these genera by integrating gene sequences with morphological data from microscopic teeth and internal shells, the only hard parts in these soft-bodied invertebrates...
March 7, 2018: Molecular Phylogenetics and Evolution
Min Zhou, Xinjian Liu, Zonghai Li, Qian Huang, Fang Li, Chuan-Yuan Li
Caspase-3 (CASP3) is a major mediator of apoptosis activated during cellular exposure to cytotoxic drugs, radiotherapy, or immunotherapy. It is often used as a marker for efficacy of cancer therapy. However, recent reports indicate that caspase-3 also has non-apoptotic roles such as promotion of tumor relapse and tumor angiogenesis. Therefore, the roles of caspase-3 in tumor progression remains to be defined clearly. In this study, we established caspase-3 knockout (KO) colon cancer cell lines by use of the CRISPR technology...
March 10, 2018: International Journal of Cancer. Journal International du Cancer
Zachariah J Sperry, Kyounghwan Na, Saman S Parizi, Hillel J Chiel, John Seymour, Euisik Yoon, Tim M Bruns
The dorsal root ganglia (DRG) are promising nerve structures for sensory neural interfaces because they provide centralized access to primary afferent cell bodies and spinal reflex circuitry. In order to harness this potential, new electrode technologies are needed which take advantage of the unique properties of DRG, specifically the high density of neural cell bodies at the dorsal surface. Here we report initial <i>in vivo</i> results from the development of a flexible non-penetrating polyimide electrode array interfacing with the surface of ganglia...
March 9, 2018: Journal of Neural Engineering
Liming Dong, Yanpei Li, Dongwei Xue, Yili Liu
The role of protein l-isoaspartate (d-aspartate) O-methyltransferase (PCMT1) in human cancer was generally cognized. The clinical significance and biological function of PCMT1 in bladder cancer is still unknown. PCMT1 mRNA and protein expression levels in bladder cancer tissues and cell lines were detected by qRT-PCR, immunohistochemistry, or western blot. The correlation between PCMT1 expression and clinicopathological factors was analyzed through immunohistochemistry in 108 bladder cancer patients. Loss-of-function and gain-of-function studies were conducted to explore the biological function of PCMT1 in bladder cancer cell lines in regulating cell proliferation, migration, and invasion...
March 8, 2018: IUBMB Life
Bang-Tang Yin, Xiang-Fang Li, Gang Liu
The most prominent aspect of multiphase flow is the variation in the physical distribution of the phases in the flow conduit known as the flow pattern. Several different flow patterns can exist under different flow conditions which have significant effects on liquid holdup, pressure gradient and heat transfer. Gas-liquid two-phase flow in an annulus can be found in a variety of practical situations. In high rate oil and gas production, it may be beneficial to flow fluids vertically through the annulus configuration between well tubing and casing...
2018: Petroleum Science
Yuka Yoshida, Munenori Ide, Hiroya Fujimaki, Nozomi Matsumura, Sumihito Nobusawa, Hayato Ikota, Hideaki Yokoo
A 51-year-old man presented with a 2-week history of malaise. MRI revealed a large solid and cystic lesion with ring enhancement measuring 6.5 cm in diameter in the right frontal lobe. Histologically, the tumor consisted of various components: diffuse growth of atypical astrocytic cells consistent with glioblastoma, fascicular proliferation of atypical spindle cells such as fibrosarcoma, clusters of primitive neuronal cells, and foci of ependymal cells. The sarcomatous component also focally exhibited chondroid and osteoid differentiation...
March 5, 2018: Neuropathology: Official Journal of the Japanese Society of Neuropathology
Jeffrey W Brown, Derek Caetano-Anollés, Marianne Catanho, Ekaterina Gribkova, Nathaniel Ryckman, Kun Tian, Mikhail Voloshin, Rhanor Gillette
Economic decisions arise from evaluation of alternative actions in contexts of motivation and memory. In the predatory sea-slug Pleurobranchaea the economic decisions of foraging are found to occur by the workings of a simple, affectively controlled homeostat with learning abilities. Here, the neuronal circuit relations for approach-avoidance choice of Pleurobranchaea are expressed and tested in the foraging simulation Cyberslug. Choice is organized around appetitive state as a moment-to-moment integration of sensation, motivation (satiation/hunger), and memory...
January 2018: ENeuro
Alex Morris, Michael Green, Hayley Martin, Katie Crossland, William T Swaney, Sally M Williamson, Robbie Rae
The ability of parasites to manipulate the behaviour of their hosts has evolved multiple times, and has a clear fitness benefit to the parasite in terms of facilitating growth, reproduction and transfer to suitable hosts. The mechanisms by which these behavioural changes are induced are poorly understood, but in many cases parasite manipulation of serotonergic signalling in the host brain is implicated. Here we report that Phasmarhabditis hermaphrodita, a parasite of terrestrial gastropod molluscs, can alter the behaviour of slugs...
February 27, 2018: Behavioural Processes
Chao Wang, Pengcheng Liu, Yanling Wang, Zhe Yuan, Zhenhua Xu
A novel synthetic hydrophobically modified hydroxyethyl cellulose (HEC) using bromododecane (BD) was developed in our previous paper, which we denote as BD-HMHEC. A series of one dimensional core displacement experiments were continually conducted to evaluate the key effect factors on the resistance factor (FR ) and residual resistance factor (FRR ) of BD-HMHEC solution, including polymer concentration, core permeability and injection rate. Results have shown that BD-HMHEC has higher FR and FRR and has much better oil displacement performance than HEC during oil displacement process...
March 1, 2018: Scientific Reports
Shuling Zhou, Xiangjie Sun, Lin Yu, Ruoji Zhou, Anqi Li, Ming Li, Wentao Yang
Background: Triple-negative breast cancer (TNBC) is a heterogeneous disease closely associated with epithelial-to-mesenchymal transition (EMT). This study aimed to investigate the role of EMT in metaplastic carcinoma. Methods: E-cadherin, Slug, Twist and Vimentin levels were detected by immunohistochemistry in 167 TNBC tumors, including 145 invasive carcinomas of no special type (ICONSTs), 14 spindle cell carcinomas (SpCCs) and 8 matrix-producing carcinomas (MPCs). Results: Nuclear Slug and Twist were more frequently detected in SpCC and MPC tumors than that in ICONST tumors (p<0...
2018: Journal of Cancer
Yan Huang, Sun Wu, Yuan Zhang, Lihua Wang, Yan Guo
Introduction: T-cell lymphoblastic lymphoma (T-LBL) is a widely disseminated disease worldwide. Triptolide (TPL) is purified from Chinese herb and displays anti-inflammatory, anti-fertility, anti-tumor and immunosuppressive effects. Materials and methods: Here, in vitro and in vivo experiments were conducted to investigate the anti-tumor effect of TPL treatment in T-LBL and the potential mechanism in T-LBL progression. Results: TPL inhibited cell proliferation of T-LBL cells (Jurkat cells and Molt-3 cells) in a dose-dependent manner...
2018: OncoTargets and Therapy
Yu Shi, Jiejing Li, Chunjiang Chen, Yongwu Xia, Yanxi Li, Pan Zhang, Ying Xu, Tingyu Li, Weihui Zhou, Weihong Song
Ketamine is a potent dissociative anesthetic and the most commonly used illicit drug. Many addicts are women at childbearing age. Although ketamine has been extensively studied as a clinical anesthetic, its effects on embryonic development are poorly understood. Here, we applied the Xenopus model to study the effects of ketamine on development. We found that exposure to ketamine from pre-gastrulation (stage 7) to early neural plate (stage 13.5) resulted in disruption of neural crest (NC) derivatives. Ketamine exposure did not affect mesoderm development as indicated by the normal expression of Chordin, Xbra, Wnt8, and Fgf8...
2018: Frontiers in Molecular Neuroscience
M Ioannou, E Kouvaras, R Papamichali, M Samara, I Chiotoglou, G Koukoulis
Epithelial-mesenchymal transition (EMT) plays an important role in cancer metastasis. During EMT, tumor cells acquire the capacity to migrate and invade the stroma. Activation of the transforming growth factor-b (TGF-b) signaling pathway is of major importance for the initiation of EMT. Smad4, an essential protein of this pathway, is known to complex with multiple transcription factors (e.g. Snail-1, Slug, Twist-1), in various types of cancer, promoting the repression or activation of target genes. The role of Smad4 in colorectal cancer (CRC) is not straightforward so far...
February 21, 2018: Journal of Molecular Histology
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