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https://www.readbyqxmd.com/read/28231501/hsp70-expression-in-biomphalaria-glabrata-snails-exposed-to-cadmium
#1
Rebeca da Silva Cantinha, Sueli Ivone Borrely, Nancy Oguiura, Carlos Alberto de Bragança Pereira, Marcela M Rigolon, Eliana Nakano
In this study, the effects of the heavy metal cadmium on the stress protein HSP70 are investigated in freshwater mollusks Biomphalaria glabrata. Adult snails were exposed for 96h to CdCl2 at concentrations ranging from 0.09 to 0.7mgL(-1) (LC50/96h=0.34 (0.30-0.37). Time and concentration-dependent increases in the expression of HSP70 were observed at sub-lethal levels in the immunoblotting assay. Further, an increased survival to a lethal heat shock was observed in animals pre-exposed to a nonlethal concentration of cadmium, evidencing the induction of acquired tolerance...
February 20, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28228093/comparative-profiling-of-hepatopancreas-transcriptomes-in-satiated-and-starving-pomacea-canaliculata
#2
Lei Yang, Tian-Yin Cheng, Fei-Yan Zhao
BACKGROUND: Although Pomacea canaliculata is native to South and Central America, it has become one of the most abundant invasive species worldwide and causes extensive damage to agriculture and horticulture. Conventional physical and chemical techniques have been used to eliminate P. canaliculata, but the effects are not ideal. Therefore, it is important to devise a new method based on a greater understanding of the biology of P. canaliculata. However, the molecular mechanisms underlying digestion and absorption in P...
February 22, 2017: BMC Genetics
https://www.readbyqxmd.com/read/28222434/a-feedback-loop-between-mir-30a-c-5p-and-dnmt1-mediates-cisplatin-resistance-in-ovarian-cancer-cells
#3
Xi Han, Shuai Zhen, Zhongxue Ye, Jiaojiao Lu, Lijie Wang, Pan Li, Jie Li, Xia Zheng, Huijin Li, Wei Chen, Le Zhao, Xu Li
BACKGROUND: Many microRNAs (miRs) are dysregulated in cancers, and aberrant miR expression patterns have been suggested to correlate with chemo-resistance of cancer cells. We aim to study the role of miR-30 family members in cisplatin-resistance of ovarian cancer cells. METHODS: qRT-PCR was used to compare differential expression levels of miR-30 family members in ovarian cancer cell line A2780 and its cisplatin-resistant derivative CP70. Changes of cisplatin-sensitivity in miR-30a-5p- and miR-30c-5p-overexpressed-CP70 cells and miR-30a-5p- and miR-30c-5p-inhibited-A2780 cells were examined by CCK8 assay and apoptosis analysis using flow cytometry; targets of miR-30a/c-5p were analyzed by western blotting and luciferase reporter assay; methylation regulation of pre-miR-30a/c-5p was examined by methylation specific PCR...
February 21, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28221831/behavioral-hypervolumes-of-predator-groups-and-predator-predator-interactions-shape-prey-survival-rates-and-selection-on-prey-behavior
#4
Jonathan N Pruitt, Kimberly A Howell, Shaniqua J Gladney, Yusan Yang, James L L Lichtenstein, Michelle Elise Spicer, Sebastian A Echeverri, Noa Pinter-Wollman
Predator-prey interactions often vary on the basis of the traits of the individual predators and prey involved. Here we examine whether the multidimensional behavioral diversity of predator groups shapes prey mortality rates and selection on prey behavior. We ran individual sea stars (Pisaster ochraceus) through three behavioral assays to characterize individuals' behavioral phenotype along three axes. We then created groups that varied in the volume of behavioral space that they occupied. We further manipulated the ability of predators to interact with one another physically via the addition of barriers...
March 2017: American Naturalist
https://www.readbyqxmd.com/read/28221828/does-cohistory-constrain-information-use-evidence-for-generalized-risk-assessment-in-nonnative-prey
#5
Emily W Grason
Though prey use a variety of information sources to assess predation risk, evolutionary cohistory with a predator could constrain information use, and nonnative prey might fail to recognize risk from a novel predator. Nonnative prey might instead use generalized risk assessment, relying on general alarm signals from injured conspecifics rather than cues from predators. I tested the influence of shared predator-prey history on information use, comparing responses among three native and four nonnative prey species to chemical cues from a native predator and cues from injured conspecific prey...
March 2017: American Naturalist
https://www.readbyqxmd.com/read/28220242/expression-of-clonorchis-sinensis-giiispla2-protein-in-baculovirus-infected-insect-cells-and-its-overexpression-facilitating-epithelial-mesenchymal-transition-in-huh7-cells-via-akt-pathway
#6
Mei Shang, Zhizhi Xie, Zeli Tang, Lei He, Xiaoyun Wang, Caiqin Wang, Yinjuan Wu, Ye Li, Lu Zhao, Zhiyue Lv, Zhongdao Wu, Yan Huang, Xinbing Yu, Xuerong Li
Although prior studies confirmed that group III secretory phospholipase A2 of Clonorchis sinensis (CsGIIIsPLA2) had stimulating effect on liver fibrosis by binding to LX-2 cells, large-scale expression of recombinant protein and its function in the progression of hepatoma are worth exploring. Because of high productivity and low lipopolysaccharides (LPS) in the Sf9-baculovirus expression system, we firstly used this system to express the coding region of CsGIIIsPLA2. The molecular weight of recombinant CsGIIIsPLA2 protein was about 34 kDa...
February 20, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28219411/using-the-hierarchical-ordinal-regression-model-to-analyse-the-intensity-of-urinary-schistosomiasis-infection-in-school-children-in-lusaka-province-zambia
#7
Christopher Simoonga, Lawrence N Kazembe
BACKGROUND: Urinary schistosomiasis has been a major public health problem in Zambia for many years. However, the disease profile may vary in different locale due to the changing ecosystem that contributes to the risk of acquiring the disease. The objective of this study was to quantify risk factors associated with the intensity of urinary schistosomiasis infection in school children in Lusaka Province, Zambia, in order to better understand local transmission. METHODS: Data were obtained from 1 912 school children, in 20 communities, in the districts of Luangwa and Kafue in Lusaka Province...
February 21, 2017: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/28216409/structure-and-activity-of-contryphan-vc2-importance-of-the-d-amino-acid-residue
#8
Stephen B Drane, Samuel D Robinson, Christopher A MacRaild, Sandeep Chhabra, Balasubramanyam Chittoor, Rodrigo A V Morales, Eleanor W W Leung, Alessia Belgi, Samuel S Espino, Baldomero M Olivera, Andrea J Robinson, David K Chalmers, Raymond S Norton
In natural proteins and peptides, amino acids exist almost invariably as L-isomers. There are, however, several examples of naturally-occurring peptides containing D-amino acids. In this study we investigated the role of a naturally-occurring D-amino acid in a small peptide identified in the transcriptome of a marine cone snail. This peptide belongs to a family of peptides known as contryphans, all of which contain a single D-amino acid residue. The solution structure of this peptide was solved by NMR, but further investigations with molecular dynamics simulations suggest that its solution behaviour may be more dynamic than suggested by the NMR ensemble...
February 16, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28215862/natural-infection-of-the-feline-lungworm-aelurostrongylus-abstrusus-in-the-invasive-snail-achatina-fulica-from-argentina
#9
Romina Valente, Julia Ines Diaz, Oscar Daniel Salomón, Graciela Teresa Navone
The giant African snail Achatina fulica is an invasive mollusk native to Africa, the first record in Argentina was in Puerto Iguazú, in northeastern Argentina in 2010. Recently it was reported in Corrientes Province. This snail can act as an intermediate host of Metastrongyloidea nematodes of importance in public health as: Angiostrongylus cantonensis, Angiostrongylus costaricensis and Angiostrongylus vasorum. Taking into account the presence of A. fulica in Argentina, the objectives of this study is to assess the presence of Metastrongyloidea nematodes in this mollusk species in Puerto Iguazú, Misiones, close to the international border with Brazil and Paraguay...
February 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28215859/evaluation-of-molecular-methods-for-the-field-study-of-the-natural-history-of-dicrocoelium-dendriticum
#10
Gillian Mitchell, Grace Cuthill, Alex Haine, Ruth Zadoks, Umer Chaudhry, Philip Skuce, Neil Sargison
There is a need for improved methods for the study of the impacts of climatic and livestock management change on the epidemiology of production-limiting helminth parasitic diseases. In this study we report the application of molecular methods to describe the natural history of the small lancet fluke, Dicrocoelium dendriticum on Machair pastures on the Inner Hebridean Isle of Coll. Our results build upon those of the only previous historic field study of D. dendriticum in the British Isles that had been undertaken on the same study site...
February 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28215642/ccl2-expression-correlates-with-snail-expression-and-affects-the-prognosis-of-patients-with-gastric-cancer
#11
Jia Zhang, Yan Yan, Xiaohai Cui, Jing Zhang, Ya Yang, Huajing Li, Hongpei Wu, Junhai Li, Li Wang, Min Li, Xu Liu, Jiansheng Wang, Xiaoyi Duan
We aim to explore the associations of CCL2 and Snail in gastric cancer to the clinicopathologic features and prognosis of gastric cancer (GC). In our study, the expression of CCL2 and Snail in clinical specimens of 178 GC patients was detected by immunohistochemistry. High expression of CCL2 and Snail were closely related to the clinicopathologic features. The results showed there is a link between CCL2 and Snail expression at protein levels (Pearson Χ2=40.751, P<0.001). The Kaplan-Meier survival analysis showed that CCL2 or Snail expression was correlated with 5-year survival rate (P<0...
December 23, 2016: Pathology, Research and Practice
https://www.readbyqxmd.com/read/28214876/the-snail-family-in-normal-and-malignant-haematopoiesis
#12
Catherine L Carmichael, Jody J Haigh
Snail family proteins are key inducers of the epithelial-mesenchymal transition (EMT), a critical process required for normal embryonic development. They have also been strongly implicated in regulating the EMT-like processes required for tumour cell invasion, migration, and metastasis. Whether these proteins also contribute to normal blood cell development, however, remains to be clearly defined. Increasing evidence supports a role for the Snail family in regulating cell survival, migration, and differentiation within the haematopoietic system, as well as potentially an oncogenic role in the malignant transformation of haematopoietic stem cells...
2017: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/28214216/cancer-stem-cell-cytokeratins-and-epithelial-to-mesenchymal-transition-markers-expression-in-oral-squamous-cell-carcinoma-derived-from-ortothopic-xenoimplantation-of-cd44-high-cells
#13
Nathália Paiva de Andrade, Maria Fernanda Setúbal Destro Rodrigues, Camila Oliveira Rodini, Fabio Daumas Nunes
Oral squamous cell carcinoma (OSCC) is the most prevalent neoplasia of oral cavity worldwide and prognosis remains unchanged in decades. Recently, different authors reported that head and neck squamous cell carcinomas have a subpopulation of tumor initiating cells that apparently correspond to cancer stem cells (CSC) and are also responsible for tumor growth and metastasis. The purpose of the present study was to investigate the microscopic and phenotypic characteristics of OSCC tumors induced after orthotopic xenoimplantation of SCC9(WT) cell line and CSC-enriched subpopulation isolated from SCC9 cell line based on high expression of the putative CSC marker CD44...
December 23, 2016: Pathology, Research and Practice
https://www.readbyqxmd.com/read/28212929/structural-analysis-of-a-homogeneous-polysaccharide-from-achatina-fulica
#14
Jie Liu, Feineng Shang, Zengming Yang, Mingyi Wu, Jinhua Zhao
Edible snails have been widely used as a health food and medicine in many countries. In our study, a water-soluble polysaccharide (AF-1) was isolated and purified from Achatina fulica by papain enzymolysis, alcohol precipitation and strong anion exchange chromatography. Structureof the polysaccharide was analyzed and characterized by chemical and instrumental methods, such as Fourier transform infrared spectroscopy, high performance liquid chromatography, analysis of monosaccharide composition, methylation analysis, and nuclear magnetic resonance (NMR) spectroscopy ((1)H, (13)C, COSY, TOCSY, NOESY, HSQC and HMBC)...
February 15, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28212558/ataxin-1-regulates-epithelial-mesenchymal-transition-of-cervical-cancer-cells
#15
A-Ram Kang, Hyoung-Tae An, Jesang Ko, Seongman Kang
The mutant form of the protein ataxin-1 (ATXN1) causes the neurodegenerative disease spinocerebellar ataxia type-1. Recently, ATXN1 was reported to enhance E-cadherin expression in the breast cancer cell line MCF-7, suggesting a potential association between ATXN1 and cancer development. In the present study, we discovered a novel mechanism through which ATXN1 regulates the epithelial-mesenchymal transition (EMT) of cancer cells. Hypoxia-induced upregulation of the Notch intracellular domain expression decreased ATXN1 expression via MDM2-associated ubiquitination and degradation...
February 14, 2017: Oncotarget
https://www.readbyqxmd.com/read/28211758/life-cycle-host-utilization-and-ecological-fitting-for-invasive-lancet-liver-fluke-dicrocoelium-dendriticum-emerging-in-southern-alberta-canada
#16
Bradley van Paridon, John S Gilleard, Douglas D Colwell, Cameron P Goater
The expansion of parasite distributions outside of their native host and geographical ranges has occurred repeatedly over evolutionary time. Contemporary examples include emerging infectious diseases (EID's), many of which pose threats to human, domestic animal, and wildlife populations. Theory predicts that parasites with complex life cycles will be rare as EID's due to constraints imposed by host specialization at each life-cycle stage. In contrast to predictions of this theory, we report 2 new intermediate hosts in the 3-host life cycle of the liver fluke Dicrocoelium dendriticum in Cypress Hills Provincial Park, Alberta, Canada...
February 17, 2017: Journal of Parasitology
https://www.readbyqxmd.com/read/28209945/effects-of-static-cold-storage-and-hypothermic-machine-perfusion-on-oxidative-stress-factors-adhesion-molecules-and-zinc-finger-transcription-factor-proteins-before-and-after-liver-transplantation
#17
De-Fang Zhao, Qin Dong, Tong Zhang
BACKGROUND This study aimed to investigate the effects of static cold storage (SCS) and hypothermic machine perfusion (HMP) on the oxidative stress factors (OSF), adhesion molecules (AM), and zinc finger transcription factor (Snail) before and after liver transplantation. MATERIAL AND METHODS Experimental dogs were randomly divided into donor (group A), SCS (group B), and HMP (group C) (n=30) groups. Livers retrieved from group A were transplanted into group B after SCS, and the livers sampled from group B were transplanted into group C after HMP...
February 17, 2017: Annals of Transplantation: Quarterly of the Polish Transplantation Society
https://www.readbyqxmd.com/read/28209612/microrna-520f-reverses-epithelial-to-mesenchymal-transition-by-targeting-adam9-and-tgfbr2
#18
Jasmijn Gm van Kampen, Onno van Hooij, Cornelius Fj Jansen, Frank P Smit, Paula I van Noort, Iman J Schultz, Roel Qj Schaapveld, Jack A Schalken, Gerald W Verhaegh
Reversing epithelial-to-mesenchymal transition (EMT) in cancer cells has been widely considered as an approach to combat cancer progression and therapeutic resistance, but a limited number of broadly comprehensive investigations of microRNAs involved in this process have been conducted. In this study, we screened a library of 1120 microRNA for their ability to transcriptionally activate the E-cadherin gene CDH1 in a promoter reporter assay as a measure of EMT reversal. By this approach, we defined miR-520f as a novel EMT-reversing microRNA...
February 16, 2017: Cancer Research
https://www.readbyqxmd.com/read/28209530/slc39a6-promotes-aggressiveness-of-esophageal-carcinoma-cells-by-increasing-intracellular-levels-of-zinc-activating-phosphatidylinositol-3-kinase-signaling-and-upregulating-genes-that-regulate-metastasis
#19
Xinxin Cheng, Lixuan Wei, Xudong Huang, Jian Zheng, Mingming Shao, Ting Feng, Jun Li, Yaling Han, Wenle Tan, Wen Tan, Dongxin Lin, Chen Wu
BACKGROUND & AIMS: A common variant in the solute carrier family 39 member 6 gene (SLC39A6) has been associated with survival times of patients with esophageal squamous-cell carcinoma (ESCC). We investigated the function of SLC39A6 and ways in which this variant affects tumor progression by studying ESCC samples and cell lines. METHODS: SLC39A6 was expressed, or knocked down by expression of small hairpin RNAs, in ESCC cells (KYSE30 and KYSE450) and HeLa cells using lentiviral vectors; we analyzed effects on proliferation, colony formation, migration, and invasion in vitro...
February 13, 2017: Gastroenterology
https://www.readbyqxmd.com/read/28208423/modeling-angiogenesis-a-discrete-to-continuum-description
#20
Samara Pillay, Helen M Byrne, Philip K Maini
Angiogenesis is the process by which new blood vessels develop from existing vasculature. During angiogenesis, endothelial tip cells migrate via diffusion and chemotaxis, loops form via tip-to-tip and tip-to-sprout anastomosis, new tip cells are produced via branching, and a vessel network forms as endothelial cells follow the paths of tip cells. The latter process is known as the snail trail. We use a mean-field approximation to systematically derive a continuum model from a two-dimensional lattice-based cellular automaton model of angiogenesis in the corneal assay, based on the snail-trail process...
January 2017: Physical Review. E
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