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Markers of endothelial injury

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https://www.readbyqxmd.com/read/28933966/mobile-laser-indirect-ophthalmoscope-for-the-induction-of-choroidal-neovascularization-in-a-mouse-model
#1
Dov Weinberger, Elite Bor-Shavit, Tilda Barliya, Mor Dahbash, Opher Kinrot, Dan D Gaton, Yael Nisgav, Tami Livnat
PURPOSE: This study aims to evaluate and standardize the reliability of a mobile laser indirect ophthalmoscope in the induction of choroidal neovascularization (CNV) in a mouse model. MATERIALS & METHODS: A diode laser indirect ophthalmoscope was used to induce CNV in pigmented male C57BL/6J mice. Standardization of spot size and laser intensity was determined using different aspheric lenses with increasing laser intensities applied around the optic disc. Development of CNV was evaluated 1, 5, and 14 days post laser application using fluorescein angiography (FA), histology, and choroidal flat mounts stained for the endothelial marker CD31 and FITC-dextran...
September 21, 2017: Current Eye Research
https://www.readbyqxmd.com/read/28930996/assessment-of-the-anticoagulant-and-anti-inflammatory-properties-of-endothelial-cells-using-3d-cell-culture-and-non-anticoagulated-whole-blood
#2
Riccardo Sfriso, Anjan Bongoni, Yara Banz, Nikolai Klymiuk, Eckhard Wolf, Robert Rieben
In vivo, endothelial cells are crucial for the natural anticoagulation of circulating blood. Consequently, endothelial cell activation leads to blood coagulation. This phenomenon is observed in many clinical situations, like organ transplantation in the presence of pre-formed anti-donor antibodies, including xenotransplantation, as well as in ischemia/reperfusion injury. In order to reduce animal experimentation according to the 3R standards (reduction, replacement and refinement), in vitro models to study the effect of endothelial cell activation on blood coagulation would be highly desirable...
September 5, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28930962/attenuation-of-hemorrhage-associated-lung-injury-by-adjuvant-treatment-with-c23-an-oligopeptide-derived-from-cold-inducible-rna-binding-protein
#3
Fangming Zhang, Weng-Lang Yang, Max Brenner, Ping Wang
BACKGROUND: Hemorrhagic shock (HS) is an important cause of mortality. HS is associated with an elevated incidence of acute lung injury and acute respiratory distress syndrome, significantly contributing to HS morbidity and mortality. Cold-inducible RNA-binding protein (CIRP) is released into the circulation during HS and can cause lung injury. C23 is a CIRP-derived oligopeptide that binds with high affinity to the CIRP receptor and inhibits CIRP-induced phagocyte secretion of TNF-α...
October 2017: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/28924363/thrombospondin-1-gene-deficiency-worsens-the-neurological-outcomes-of-traumatic-brain-injury-in-mice
#4
Chongjie Cheng, Zhanyang Yu, Song Zhao, Zhengbu Liao, Changhong Xing, Yinghua Jiang, Yong-Guang Yang, Michael J Whalen, Eng H Lo, Xiaochuan Sun, Xiaoying Wang
Background: Thrombospondin-1 (TSP-1) is an extracellular matrix protein that plays multiple physiological and pathophysiological roles in the brain. Experimental reports suggest that TSP-1 may have an adverse role in neuronal function recovery under certain injury conditions. However, the roles of TSP-1 in traumatic brain injury (TBI) have not been elucidated. In this study we for the first time investigated the roles of TSP-1 in a controlled cortical impact (CCI) model of TBI in TSP-1 knockout (TSP-1 KO) and wild type (WT) mice...
2017: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/28918481/fibrinolysis-in-trauma-a-review
#5
REVIEW
M J Madurska, K A Sachse, J O Jansen, T E Rasmussen, J J Morrison
Fibrinolytic dysregulation is an important mechanism in traumatic coagulopathy. It is an incompletely understood process that consists of a spectrum ranging from excessive breakdown (hyperfibrinolysis) and the shutdown of fibrinolysis. Both hyperfibrinolysis and shutdown are associated with excess mortality and post-traumatic organ failure. The pathophysiology appears to relate to endothelial injury and hypoperfusion, with several molecular markers identified in playing a role. Although there are no universally accepted diagnostic tests, viscoelastic studies appear to offer the greatest potential for timely identification of patients presenting with fibrinolytic dysregulation...
September 16, 2017: European Journal of Trauma and Emergency Surgery: Official Publication of the European Trauma Society
https://www.readbyqxmd.com/read/28915536/protective-effect-of-the-standardized-extract-of-ginkgo-biloba-egb761-against-hypertension-with-hypercholesterolemia-induced-renal-injury-in-rats-insights-in-the-underlying-mechanisms
#6
Ahmed O Abdel-Zaher, Hanan S M Farghaly, Abeer E M El-Refaiy, Ahmed M Abd-Eldayem
The potential protective role of the standardized leaf extract of ginkgo biloba (EGb761) on hypertension with hypercholesterolemia-induced renal injury was investigated in rats. Hypertension was induced by L-N(G)-nitroarginine methyl ester (L-NAME) and hypercholesterolemia was induced by feeding rats with a diet containing 1% cholesterol. In these animals repeated treatment with EGb761 produced a progressive reduction in the systolic, diastolic and mean arterial blood pressure (BP). EGb761 increased the progressive reduction in the systolic, diastolic and mean arterial BP induced by repeated administration of losartan with simvastatin...
September 11, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28900088/-estimation-of-time-dependent-microrna-expression-patterns-in-brain-tissue-leukocytes-and-blood-plasma-of-rats-under-photochemically-induced-focal-cerebral-ischemia
#7
V A Gusar, A V Timofeeva, I S Zhanin, S I Shram, V G Pinelis
miRNA expression over different time periods (24 and 48 h) using the quantitative RT-PCR and deep sequencing has been evaluated in a model of photochemically induced thrombosis. A combination of two approaches allowed us to determine the miRNA expression patterns caused by ischemia. Nine miRNAs, including let-7f-5p, miR-221-3p, miR-21-5p, miR-30c-5p, miR-30a-3p, miR-223-3p, miR-23a-3p, miR-22-5p, and miR-99a-5p, were differentially expressed in brain tissue and leukocytes of rats 48 h after onset of ischemia...
July 2017: Molekuliarnaia Biologiia
https://www.readbyqxmd.com/read/28886695/cerebral-microvascular-dysfunction-in-metabolic-syndrome-is-exacerbated-by-ischemia-reperfusion-injury
#8
Nathalie Obadia, Marcos Adriano Lessa, Anissa Daliry, Raquel Rangel Silvares, Fabiana Gomes, Eduardo Tibiriçá, Vanessa Estato
BACKGROUND: Metabolic syndrome (MetS) is associated with an increased risk of cerebrovascular diseases, including cerebral ischemia. Microvascular dysfunction is an important feature underlying the pathophysiology of cerebrovascular diseases. In this study, we aimed to investigate the impacts of ischemia and reperfusion (IR) injury on the cerebral microvascular function of rats with high-fat diet-induced MetS. RESULTS: We examined Wistar rats fed a high-fat diet (HFD) or normal diet (CTL) for 20 weeks underwent 30 min of bilateral carotid artery occlusion followed by 1 h of reperfusion (IR) or sham surgery...
September 8, 2017: BMC Neuroscience
https://www.readbyqxmd.com/read/28885470/poor-microcirculatory-flow-dynamics-are-associated-with-endothelial-cell-damage-and-glycocalyx-shedding-following-traumatic-hemorrhagic-shock
#9
David N Naumann, Jon Hazeldine, Mark J Midwinter, Sam D Hutchings, Paul Harrison
BACKGROUND: Endothelial cell damage and glycocalyx shedding following trauma can increase the risk of inflammation, coagulopathy, vascular permeability and death. Bedside sublingual video-microscopy may detect worse flow and perfusion associated with this endotheliopathy. We compared markers of endotheliopathy with physical flow dynamics following traumatic hemorrhagic shock (THS). METHODS: Sublingual incident dark field video-microscopy was performed at three time-points following injury (<10h; 10-30h; and 30-50h)...
September 5, 2017: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/28880966/the-prolyl-4-hydroxylase-inhibitor-gsk360a-decreases-post-stroke-brain-injury-and-sensory-motor-and-cognitive-behavioral-deficits
#10
Jin Zhou, Jie Li, Daniel M Rosenbaum, Jian Zhuang, Carrie Poon, Pu Qin, Katrina Rivera, John Lepore, Robert N Willette, Erding Hu, Frank C Barone
There is interest in pharmacologic preconditioning for end-organ protection by targeting the HIF system. This can be accomplished by inhibition of prolyl 4-hydroxylase (PHD). GSK360A is an orally active PHD inhibitor that has been previously shown to protect the failing heart. We hypothesized that PHD inhibition can also protect the brain from injuries and resulting behavioral deficits that can occur as a result of surgery. Thus, our goal was to investigate the effect of pre-stroke surgery brain protection using a verified GSK360A PHD inhibition paradigm on post-stroke surgery outcomes...
2017: PloS One
https://www.readbyqxmd.com/read/28870649/lipopolysaccharide-impairs-permeability-of-pulmonary-microvascular-endothelial-cells-via-connexin40
#11
Hua-Song Zhou, Meng Li, Bing-Dong Sui, Lei Wei, Rui Hou, Wen-Sheng Chen, Qiang Li, Sheng-Hui Bi, Jin-Zhou Zhang, Ding-Hua Yi
The endotoxin lipopolysaccharide (LPS)-induced pulmonary endothelial barrier disruption is a key pathogenesis of acute lung injury (ALI) and acute respiratory distress syndrome (ARDS). However, the molecular mechanisms underlying LPS-impaired permeability of pulmonary microvascular endothelial cells (PMVECs) are not fully understood. Gap junctions, particularly Connexin40 (Cx40), are necessary for the maintenance of normal vascular function. In this study, we for the first time investigated the role of Cx40 in LPS-impaired permeability of PMVECs and provided potential therapeutic approaches based on mechanistic findings of Cx40 regulation by LPS stimuli...
September 1, 2017: Microvascular Research
https://www.readbyqxmd.com/read/28867452/the-role-of-osteoprotegerin-in-the-crosstalk-between-vessels-and-bone-its-potential-utility-as-a-marker-of-cardiometabolic-diseases
#12
REVIEW
Luc Rochette, Alexandre Meloux, Eve Rigal, Marianne Zeller, Yves Cottin, Catherine Vergely
Among the numerous molecules that are being studied for their potential utility as biomarkers of cardiovascular diseases, much interest has been shown in the superfamily of tumor necrosis factor (TNF) receptors. Members of this family include osteoprotegerin (OPG) and its ligands, which are receptor activators of nuclear factor κB ligand (RANKL) and TNF-related apoptosis-inducing ligand (TRAIL). These signals may be expressed and regulated, and their functions could be involved in several physiological and pathological processes...
September 1, 2017: Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/28864209/danshensu-accelerates-angiogenesis-after-myocardial-infarction-in-rats-and-promotes-the-functions-of-endothelial-progenitor-cells-through-sdf-1%C3%AE-cxcr4-axis
#13
Ying Yin, Jialin Duan, Chao Guo, Guo Wei, Yanhua Wang, Yue Guan, Fei Mu, Minna Yao, Miaomiao Xi, Aidong Wen
The present study was performed to investigate the potential role of Danshensu in therapeutic angiogenesis in ischemic myocardium and endothelial progenitor cells (EPCs) function. The rat model of myocardial infarction (MI) injury was induced by left anterior descending coronary artery ligation for 14 days. Danshensu significantly alleviated myocardial ischemia injury by ameliorating left ventricular function and reducing infarct size. Furthermore, Danshensu potentiated post-ischemia neovascularization as evidenced by increased microvessel density in infarction boundary zone, as well as the expression of marker proteins vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF)...
August 31, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28854425/hydrogen-sulfide-preconditioning-of-human-endothelial-progenitor-cells-transplantation-improves-re-endothelialization-in-nude-mice-with-carotid-artery-injury
#14
Xiao Ke, Jun Zou, Qingsong Hu, Xiaoqing Wang, Chengheng Hu, Rongfeng Yang, Jiawen Liang, Xiaorong Shu, Ruqiong Nie, Changnong Peng
BACKGROUND/AIMS: The aim of present study was to test the hypothesis that preconditioning with sodium hydrosulfide (NaHS) could enhance the capacity of migration, adhesion and proliferation of endothelial progenitor cells (EPCs) in vitro, and also could improve the efficacy of EPCs transplantation for re-endothelialization in nude mice with carotid artery injury. The paper further addressed the underlying mechanisms. METHODS: EPCs were isolated from peripheral blood mononuclear cells of healthy male volunteers and the markers of EPCs were analyzed by flow cytometry...
August 31, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28846193/the-endothelial-glycocalyx-in-health-and-kidney-disease-rising-star-or-false-dawn
#15
REVIEW
Hui Liew, Matthew A Roberts, Robert MacGinley, Lawrence P McMahon
The endothelial glycocalyx is a layer comprised of proteins and carbohydrates on the luminal surface of vascular endothelial cells, thought to have an important role in the health and function of the endothelium. Disrupted by various pathophysiological conditions and linked with clinical outcomes, it is increasingly recognised as an early indicator of endothelial injury and a potential marker of vascular injury. In this review, we discuss current methods of assessment (including novel optical approaches), evidence for its use as a marker of vascular disease and its potential role in relation to microalbuminuria and glomerular endothelial dysfunction...
August 28, 2017: Nephrology
https://www.readbyqxmd.com/read/28841902/markers-of-acute-kidney-injury-in-patients-with-sepsis-the-role-of-soluble-thrombomodulin
#16
Shinshu Katayama, Shin Nunomiya, Kansuke Koyama, Masahiko Wada, Toshitaka Koinuma, Yuya Goto, Ken Tonai, Jun Shima
BACKGROUND: Endothelial activation and damage occur early during sepsis, with activated coagulopathy and playing a major role in the pathophysiology of sepsis-induced acute kidney injury (AKI). The aim of this study was to compare the various biomarkers of endothelial injury with the biomarkers of coagulation and inflammation and to determine a significant predictor of AKI in patients with sepsis. METHODS: We conducted a single-center, retrospective, observational study on patients with sepsis fulfilling the Third International Consensus Definitions for Sepsis and Septic Shock criteria admitted to an adult intensive care unit (ICU) at a university hospital from June 2011 to December 2016...
August 25, 2017: Critical Care: the Official Journal of the Critical Care Forum
https://www.readbyqxmd.com/read/28840975/cardioprotection-mediated-by-exosomes-is-impaired-in-the-setting-of-type-ii-diabetes-but-can-be-rescued-by-the-use-of-non-diabetic-exosomes-in-vitro
#17
Sean M Davidson, Jaime A Riquelme, Kaloyan Takov, Jose M Vicencio, Claire Boi-Doku, Vanessa Khoo, Christian Doreth, Dina Radenkovic, Sergio Lavandero, Derek M Yellon
Many patients with ischaemic heart disease also have diabetes. As myocardial infarction is a major cause of mortality and morbidity in these patients, treatments that increase cell survival in response to ischaemia and reperfusion are needed. Exosomes-nano-sized, lipid vesicles released from cells-can protect the hearts of non-diabetic rats. We previously showed that exosomal HSP70 activates a cardioprotective signalling pathway in cardiomyocytes culminating in ERK1/2 and HSP27 phosphorylation. Here, we investigated whether the exosomal cardioprotective pathway remains intact in the setting of type II diabetes...
August 25, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28840836/predictive-ability-of-circulating-osteoprotegerin-as-a-novel-biomarker-for-early-detection-of-acute-kidney-injury-induced-by-sepsis
#18
Mona Schaalan, Waleed Mohamed
Though significant progress has been made towards new diagnostic approaches for early detection of acute kidney injury (AKI) induced by different factors, there is still an urgent demand for a more specific and predictive biomarker for each type. The aim of this study is to unravel the potential diagnostic utility of circulating osteoprotegerin (OPG) in septic patients who developed AKI in the ICU, compared to cystatin C (a renal function maker) and KIM-1 (a kidney damage marker). Eighty patients (male = 43, female = 37) with ages ranging from 42 to 46 years and with sepsis, 40 of whom developed AKI, and 30 healthy controls were enrolled in this prospective study...
June 1, 2017: European Cytokine Network
https://www.readbyqxmd.com/read/28837705/changes-in-the-pattern-of-plasma-extracellular-vesicles-after-severe-trauma
#19
Sahithi J Kuravi, Clara M Yates, Mark Foster, Paul Harrison, Jon Hazeldine, Peter Hampson, Chris Watson, Antonio Belli, Mark Midwinter, Gerard B Nash
BACKGROUND: Extracellular vesicles (EV) released into the circulation after traumatic injury may influence complications. We thus evaluated the numbers of EV in plasma over 28 days after trauma and evaluated their pro-coagulant and inflammatory effects. METHODS AND FINDINGS: 37 patients suffering trauma with an injury severity score >15 were studied along with 24 healthy controls. Plasma samples were isolated by double centrifugation (2000g 20min; 13000g 2min) from blood collected from within an hour up to 28 days after injury...
2017: PloS One
https://www.readbyqxmd.com/read/28826366/regeneration-of-the-fallopian-tube-mucosa-using-bone-marrow-mesenchymal-stem-cell-transplantation-after-induced-chemical-injury-in-a-rat-model
#20
Shaima M Almasry, Amr K Elfayomy, Mohamed H El-Sherbiny
In this study, we describe a novel insight about the use of bone marrow-derived mesenchymal stem cells (BM-MSCs) for fallopian tube (FT) regeneration. Seventy rats' tubes were involved in this study and divided into 4 groups: control (15), ethanol injured (20), mesenchymal stem cell (MSC)-recipient without injury (15), and MSC recipient after injury (20). The BM-MSCs were isolated from male rats, and their incorporation into the tissues was confirmed by the detection of Sry gene in MSC-recipient rats using RT-PCR...
January 1, 2017: Reproductive Sciences
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