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Markers of endothelial injury

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https://www.readbyqxmd.com/read/29287059/microfluidics-a-high-throughput-system-for-the-assessment-of-the-endotheliopathy-of-trauma-and-the-effect-of-timing-of-plasma-administration-on-ameliorating-shock-associated-endothelial-dysfunction
#1
Lawrence N Diebel, Jonathan V Martin, David M Liberati
BACKGROUND: Early resuscitation after trauma-hemorrhagic shock (HS) with plasma rather than crystalloid may ameliorate systemic endothelial cell (EC) injury and dysfunction (endotheliopathy of trauma [EOT]). We postulated that endothelial lined microfluidic networks would be a useful platform to study the endothelial cell activation/injury under flow conditions to mimic HS. We then used the microfluidic system to further characterize the protective effects and optimal timing of plasma infusion on the development of "EOT" in our model...
December 28, 2017: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/29286468/analysis-of-immune-cells-in-single-sciatic-nerves-and-dorsal-root-ganglion-from-a-single-mouse-using-flow-cytometry
#2
Asa S Hidmark, Peter P Nawroth, Thomas Fleming
Nerve-resident immune cells in the peripheral nervous system (PNS) are essential to maintaining neuronal integrity in a healthy nerve. The immune cells of the PNS are affected by injury and disease, affecting the nerve function and the capacity for regeneration. Neuronal immune cells are commonly analyzed by immunofluorescence (IF). While IF is essential for determining the location of the immune cells in the nerve, IF is only semi-quantitative and the method is limited to the number of markers that can be analyzed simultaneously and the degree of surface expression...
December 6, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29284577/distinct-myocardial-targets-for-diabetes%C3%A2-therapy-in-heart-failure-with-preserved-or%C3%A2-reduced-ejection-fraction
#3
REVIEW
Walter J Paulus, Elisa Dal Canto
Noncardiac comorbidities such as diabetes mellitus (DM) have different outcomes in heart failure with preserved ejection fraction (HFpEF) compared with heart failure with reduced ejection fraction (HFrEF). These different outcomes are the result of distinct myocardial effects of DM on HFpEF and HFrEF, which relate to different mechanisms driving myocardial remodeling in each heart failure phenotype. Myocardial remodeling is driven by microvascular endothelial inflammation in HFpEF and by cardiomyocyte cell death in HFrEF...
January 2018: JACC. Heart Failure
https://www.readbyqxmd.com/read/29282647/pericytes-the-role-of-multipotent-stem-cells-in-vascular-maintenance-and-regenerative-medicine
#4
Toka A Ahmed, Nagwa El-Badri
Blood vessels consist of an inner endothelial cell layer lining the vessel wall and perivascular pericytes, also known as mural cells, which envelop the vascular tube surface. Pericytes have recently been recognized for their central role in blood vessel formation. Pericytes are multipotent cells that are heterogeneous in their origin, function, morphology and surface markers. Similar to other types of stem cells, pericytes act as a repair system in response to injury by maintaining the structural integrity of blood vessels...
December 28, 2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29282646/targeting-therapeutics-to-endothelium-are-we-there-yet
#5
REVIEW
Raisa Yu Kiseleva, Patrick M Glassman, Colin F Greineder, Elizabeth D Hood, Vladimir V Shuvaev, Vladimir R Muzykantov
Vascular endothelial cells represent an important therapeutic target in many pathologies, including inflammation, oxidative stress, and thrombosis; however, delivery of drugs to this site is often limited by the lack of specific affinity of therapeutics for these cells. Selective delivery of both small molecule drugs and therapeutic proteins to the endothelium has been achieved through the use of targeting ligands, such as monoclonal antibodies, directed against endothelial cell surface markers, particularly cell adhesion molecules (CAMs)...
December 28, 2017: Drug Delivery and Translational Research
https://www.readbyqxmd.com/read/29274587/cardioprotective-effects-of-total-flavonoids-from-jinhe-yangxin-prescription-by-activating-the-pi3k-akt-signaling-pathway-in-myocardial-ischemia-injury
#6
Yangang Cheng, Jinyan Tan, Huifeng Li, Xiangpeng Kong, Yan Liu, Rui Guo, Guoyan Li, Bingyou Yang, Miaorong Pei
The purpose of the present study was to investigate the cardioprotective effects of total flavonoids of Jinhe yangxin prescription (JHTF) on myocardial ischemia (MI) injury rats induced by Isoproterenol (ISO) and explore the potential mechanisms underlying these effects. 128 male rats were randomized into 8 groups: Control, Model, Positive, JHTF-H (2.64 g/kg/d), JHTF-M (1.32 g/kg/day), JHTF-L (0.66 g/kg/d), LY + JHTF (JHTF-H plus LY294002, an inhibitor of PI3K/Akt) and LY groups. Electrocardiogram, histopathological examination and terminal deoxynucleotidyl transferase dUTP nickend labeling (TUNEL) assay were performed...
December 20, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29274380/the-nox1-isoform-of-nadph-oxidase-is-involved-in-dysfunction-of-liver-sinusoids-in-nonalcoholic-fatty-liver-disease
#7
Misaki Matsumoto, Jia Zhang, Xueqing Zhang, Junjie Liu, Joy X Jiang, Kanji Yamaguchi, Akiyuki Taruno, Masato Katsuyama, Kazumi Iwata, Masakazu Ibi, Wenhao Cui, Kuniharu Matsuno, Yoshinori Marunaka, Yoshito Itoh, Natalie J Torok, Chihiro Yabe-Nishimura
The increased production of reactive oxygen species (ROS) has been postulated to play a key role in the progression of nonalcoholic fatty liver disease (NAFLD). However, the source of ROS and mechanisms underlying the development of NAFLD have yet to be established. We observed a significant up-regulation of a minor isoform of NADPH oxidase, NOX1, in the liver of nonalcoholic steatohepatitis (NASH) patients as well as of mice fed a high-fat and high-cholesterol (HFC) diet for 8 weeks. In mice deficient in Nox1 (Nox1KO), increased levels of serum alanine aminotransferase and hepatic cleaved caspase-3 demonstrated in HFC diet-fed wild-type mice (WT) were significantly attenuated...
December 20, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/29261771/endotheliopathy-is-associated-with-higher-levels-of-cell-free-dna-following-major-trauma-a-prospective-observational-study
#8
David N Naumann, Jon Hazeldine, Robert J Dinsdale, Jon R Bishop, Mark J Midwinter, Paul Harrison, Sam D Hutchings, Janet M Lord
BACKGROUND: Cell free deoxyribonucleic acid (cfDNA) has been proposed as a biomarker of secondary complications following trauma. Raised thrombomodulin and syndecan-1 levels have been used to indicate endotheliopathy, and are associated with inflammation, coagulopathy, and mortality. The current study aimed to analyse the association between cfDNA and biomarkers of endotheliopathy in a cohort of trauma patients, and whether raised levels of cfDNA were associated with poorer clinical outcomes...
2017: PloS One
https://www.readbyqxmd.com/read/29220696/cardioprotection-of-cape-ono2-against-myocardial-ischemia-reperfusion-induced-ros-generation-via-regulating-the-sirt1-enos-nf-%C3%AE%C2%BAb-pathway-in-vivo-and-in-vitro
#9
Dejuan Li, Xiaoling Wang, Qin Huang, Sai Li, You Zhou, Zhubo Li
Caffeic acid phenethyl ester (CAPE) could ameliorate myocardial ischemia/reperfusion injury (MIRI) by various mechanisms, but there hadn't been any reports on that CAPE could regulate silent information regulator 1 (SIRT1) and endothelial nitric oxide synthase (eNOS) to exert cardioprotective effect. The present study aimed to investigate the cardioprotective potential of caffeic acid o-nitro phenethyl ester (CAPE-oNO2) on MIRI and the possible mechanism based on the positive control of CAPE. The SD rats were subjected to left coronary artery ischemia /reperfusion (IR) and the H9c2 cell cultured in hypoxia/reoxygenation (HR) to induce the MIRI model...
November 29, 2017: Redox Biology
https://www.readbyqxmd.com/read/29210138/endovascular-shedding-markers-in-patients-with-heart-failure-with-reduced-ejection-fraction
#10
Petra Nijst, Jirka Cops, Pieter Martens, Quirine Swennen, Matthias Dupont, W H Wilson Tang, Wilfried Mullens
BACKGROUND: Endothelial glycocalyx degradation has been associated with multiple pathophysiological processes in cardiovascular disease. AIMS: To explore the role of glycocalyx shedding markers in pathophysiology of heart failure with reduced ejection fraction (HFrEF). METHODS: In 123 HFrEF patients the concentration, prognostic value and association of glycocalyx shedding markers with other disease processes was investigated. RESULTS: Median hyaluronic acid (HA) levels and syndecan-1 levels in HFrEF patients were respectively 29...
December 6, 2017: Microcirculation: the Official Journal of the Microcirculatory Society, Inc
https://www.readbyqxmd.com/read/29194316/tubastatin-a-prevents-hemorrhage-induced-endothelial-barrier-dysfunction
#11
Peter J Bruhn, Vahagn C Nikolian, Ihab Halaweish, Zhigang Chang, Martin Sillesen, Baoling Liu, Yongqing Li, Hasan B Alam
BACKGROUND: Microvascular hyperpermeability resulting from endothelial barrier dysfunction (EBD) is associated with worse clinical outcomes in trauma-induced hemorrhagic shock. We have previously shown that treatment with Tubastatin A (TubA), a histone deacetylase 6 inhibitor, improves outcomes in animal models of shock. In this study, we investigate whether TubA treatment may prevent trauma-related EBD. METHODS: Wistar-Kyoto rats subjected to 40% hemorrhage were treated with TubA or vehicle control...
November 21, 2017: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/29182425/endothelial-alterations-in-a-canine-model-of-immune-thrombocytopenia
#12
Dana N LeVine, Rachel E Cianciolo, Keith E Linder, Petra Bizikova, Adam J Birkenheuer, Marjory B Brooks, Abdelghaffar K Salous, Shila K Nordone, Dwight A Bellinger, Henry Marr, Sam L Jones, Thomas H Fischer, Yu Deng, Marshall Mazepa, Nigel S Key
Bleeding heterogeneity amongst patients with immune thrombocytopenia (ITP) is poorly understood. Platelets play a role in maintaining endothelial integrity, and variable thrombocytopenia-induced endothelial changes may influence bleeding severity. Platelet-derived endothelial stabilizers and markers of endothelial integrity in ITP are largely underexplored. We hypothesized that, in a canine ITP model, thrombocytopenia would lead to alterations in the endothelial ultrastructure and that the Von Willebrand factor (vWF) would serve as a marker of endothelial injury associated with thrombocytopenia...
November 28, 2017: Platelets
https://www.readbyqxmd.com/read/29180963/spinal-cord-molecular-and-cellular-changes-induced-by-adenoviral-vector-and-cell-mediated-triple-gene-therapy-after-severe-contusion
#13
Andrei A Izmailov, Tatyana V Povysheva, Farid V Bashirov, Mikhail E Sokolov, Filip O Fadeev, Ravil R Garifulin, Boris S Naroditsky, Denis Y Logunov, Ilnur I Salafutdinov, Yuri A Chelyshev, Rustem R Islamov, Igor A Lavrov
The gene therapy has been successful in treatment of spinal cord injury (SCI) in several animal models, although it still remains unavailable for clinical practice. Surprisingly, regardless the fact that multiple reports showed motor recovery with gene therapy, little is known about molecular and cellular changes in the post-traumatic spinal cord following viral vector- or cell-mediated gene therapy. In this study we evaluated the therapeutic efficacy and changes in spinal cord after treatment with the genes encoding vascular endothelial growth factor (VEGF), glial cell-derived neurotrophic factor (GDNF), angiogenin (ANG), and neuronal cell adhesion molecule (NCAM) applied using both approaches...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29158033/resident-fibroblast-expansion-during-cardiac-growth-and-remodeling
#14
Malina J Ivey, Jill T Kuwabara, Jonathan T Pai, Richard E Moore, Zuyue Sun, Michelle D Tallquist
Cardiac fibrosis, denoted by the deposition of extracellular matrix, manifests with a variety of diseases such as hypertension, diabetes, and myocardial infarction. Underlying this pathological extracellular matrix secretion is an expansion of fibroblasts. The mouse is now a common experimental model system for the study of cardiovascular remodeling and elucidation of fibroblast responses to cardiac growth and stress is vital for understanding disease processes. Here, using diverse but fibroblast specific markers, we report murine fibroblast distribution and proliferation in early postnatal, adult, and injured hearts...
November 20, 2017: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/29145197/inflammatory-and-angiogenic-factors-linked-to-longitudinal-microvascular-changes-in-hemodialysis-patients-irrespective-of-treatment-dose-intensity
#15
Nicos Mitsides, Tom Cornelis, Natascha J H Broers, Nanada M P Diederen, Paul Brenchley, Nicole Heitink-Ter Braak, Frank M van der Sande, Casper G Schalkwijk, Jeroen P Kooman, Sandip Mitra
BACKGROUND: Cardiovascular disease is a major contributor to the poor outcomes observed in hemodialysis. We investigated the relationship between hemodialysis intensity and vascular parameters in high-dose (HDHD; >12hrs/week) and Conventional (CHD; ≤12hrs/week) hemodialysis intensity over a 6-month period. METHODS: We present the 6-month longitudinal analysis of a 2-year multicenter study investigating the effects of HDHD on cardiovascular parameters. We used pulse wave velocity, 24hr ambulatory blood pressure and sublingual dark field capillaroscopy measurements to assess macro- and microcirculation on 6-monthly basis...
November 16, 2017: Kidney & Blood Pressure Research
https://www.readbyqxmd.com/read/29137274/effective-protection-against-acute-respiratory-distress-syndrome-sepsis-injury-by-combined-adipose-derived-mesenchymal-stem-cells-and-preactivated-disaggregated-platelets
#16
Chih-Hung Chen, Yung-Lung Chen, Pei-Hsun Sung, Cheuk-Kwan Sun, Kuan-Hung Chen, Yi-Ling Chen, Tien-Hung Huang, Hung-I Lu, Fan-Yen Lee, Jiunn-Jye Sheu, Sheng-Ying Chung, Mel S Lee, Hon-Kan Yip
This study assessed whether combining adipose-derived mesenchymal stem cells (ADMSC) with preactivated, disaggregated shape-changed platelets (PreD-SCP) was superior to either therapy alone for protecting rat lung from acute respiratory distress syndrome (ARDS) complicated by sepsis. ARDS and sepsis were induced through 100% oxygen inhalation and peritoneal administration of 1.5 mg/kg lipopolysaccharide (LPS), respectively. Adult-male Sprague-Dawley rats (n=40) were randomized into sham-control (SC), ARDS-LPS, ARDS-LPS-ADMSC (1...
October 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29134948/effect-of-scaffold-morphology-and-cell-co-culture-on-tenogenic-differentiation-of-hadmsc-on-centrifugal-melt-electrospun-poly-l%C3%A2-lactic-acid-fibrous-meshes
#17
Shaohua Wu, Hao Peng, Xiuhong Li, Philipp N Streubel, Yong Liu, Bin Duan
Engineered tendon grafts offer a promising alternative for grafting during the reconstruction of complex tendon tears. The tissue-engineered tendon substitutes have the advantage of increased biosafety and the option to customize their biochemical and biophysical properties to promote tendon regeneration. In this study, we developed a novel centrifugal melt electrospinning (CME) technique, with the goal of optimizing the fabrication parameters to generate fibrous scaffolds for tendon tissue engineering. The effects of CME processing parameters, including rotational speed, voltage, and temperature, on fiber properties (i...
November 14, 2017: Biofabrication
https://www.readbyqxmd.com/read/29133362/three-month-endothelial-human-endothelin-1-overexpression-causes-blood-pressure-elevation-and-vascular-and-kidney-injury
#18
Suellen C Coelho, Olga Berillo, Antoine Caillon, Sofiane Ouerd, Júlio C Fraulob-Aquino, Tlili Barhoumi, Stefan Offermanns, Pierre Paradis, Ernesto L Schiffrin
Endothelium-derived endothelin (ET)-1 has been implicated in the development of hypertension and end-organ damage, but its exact role remains unclear. We have shown that tamoxifen-inducible endothelium-restricted human ET-1 overexpressing (ieET-1) mice exhibited blood pressure rise after a 3-week induction in an ET type A (ETA) receptor-dependent manner, in absence of vascular and renal injury. It is unknown whether long-term ET-1 overexpression results in sustained blood pressure elevation and vascular and renal injury...
November 13, 2017: Hypertension
https://www.readbyqxmd.com/read/29129689/ascorbic-acid-attenuates-endothelial-permeability-triggered-by-cell-free-hemoglobin
#19
Jamie L Kuck, Julie A Bastarache, Ciara M Shaver, Joshua P Fessel, Sergey I Dikalov, James M May, Lorraine B Ware
BACKGROUND: Increased endothelial permeability is central to shock and organ dysfunction in sepsis but therapeutics targeted to known mediators of increased endothelial permeability have been unsuccessful in patient studies. We previously reported that cell-free hemoglobin (CFH) is elevated in the majority of patients with sepsis and is associated with organ dysfunction, poor clinical outcomes and elevated markers of oxidant injury. Others have shown that Vitamin C (ascorbate) may have endothelial protective effects in sepsis...
November 9, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29129602/standardized-fraction-of-xylocarpus-moluccensis-fruits-improve-vascular-relaxation-and-plaque-stability-in-dyslipidemic-models-of-atherosclerosis
#20
Jitendra S Kanshana, Sanjay C Rebello, Priya Pathak, Babu Nageswararao Kanuri, Hobby Aggarwal, Vasundhara Srivastava, Vivek Khanna, Vishal Singh, Kumaravelu Jagavelu, Manoj K Barthwal, Madhu Dikshit
ETHNOPHARMACOLOGICAL RELEVANCE: Xylocarpus moluccensis (Lamk.) M. Roem of family Meliaceae has triterpenoids rich fruits. Triterpenoids have been known to possess cardioprotection and anti-atherosclerotic activities (Han and Bakovic, 2015; Wu et al., 2009). Standardized fraction of these fruits exhibited anti-dyslipidemic (Srivastava et al., 2015), anti-inflammatory (Ravangpai et al., 2011) and CNS depressant activity (Sarker et al., 2007). However, there is no report in the literature on its cardiovascular effects...
November 10, 2017: Journal of Ethnopharmacology
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