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https://www.readbyqxmd.com/read/28647649/recovery-after-pilp-remineralization-of-dentin-lesions-created-with-two-cariogenic-acids
#1
K Saeki, Y-C Chien, G Nonomura, A F Chin, S Habelitz, L B Gower, S J Marshall, G W Marshall
OBJECTIVES: Acetate and lactate are important cariogenic acids produced by oral bacteria. They produced different residual dentin structures in artificial lesions of similar depth. We evaluated if such lesions responded in the same way to a polymer-induced-liquid-precursor (PILP) remineralization. DESIGN: Dentin blocks obtained from human third molars, divided into 6 groups (n=3). Blocks were demineralized with acetate (66h) or lactate (168h) buffer at pH 5.0 to create 140μm target lesion depths...
June 15, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28647374/overexpression-of-dentin-matrix-protein-1-in-nestin-cells-causes-bone-loss-in-mouse-long-bone
#2
Min Pan, Yuteng Weng, Yao Sun
The well-known matrix protein Dentin matrix protein 1 (DMP1) is expressed by osteoblasts and osteocytes in bone, and it controls bone mineralization. Recently, it has been found that DMP1 is also expressed in other cell types, such as chondrocytes. Nestin(+) cells are one important type of progenitor cell in bone marrow and are associated with bone remodeling. In our preliminary experiment, DMP1 could also be detected in Nestin(+) cells in bone marrow. This study was designed to explore the effect on bone of DMP1 in Nestin(+) cells...
June 21, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28646753/mechanical-properties-and-molecular-structure-analysis-of-subsurface-dentin-after-er-yag-laser-irradiation
#3
Zhengdi He, Lingling Chen, Xuejuan Hu, Yasushi Shimada, Masayuki Otsuki, Junji Tagami, Shuangchen Ruan
The purpose of this study was to evaluate the chemical and mechanical modifications in subsurface dentin layer after Er: YAG (Erbium-Yttrium Aluminium Garnet) laser irradiation, as the guidance of new dental restorative materials specific for laser irradiated dentin. MATERIALS AND METHODS: Dentin disks obtained from extracted human molars were prepared and exposed to a single pulse Er:YAG laser irradiation at 80mJ/pulse. After laser irradiation the mechanical and chemical characteristics of intertubular dentin in subsurface layer were studied using nanoindentation tester and micro-Raman spectromy (μ-RS)...
June 3, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28646693/antibacterial-effect-of-genetically-engineered-bacteriophage-%C3%AF-ef11-%C3%AF-fl1c-%C3%AE-36-p-nisa-on-dentin-infected-with-antibiotic-resistant-enterococcus-faecalis
#4
Justine Monnerat Tinoco, Nadia Liss, Hongming Zhang, Roni Nissan, Wanda Gordon, Eduardo Tinoco, Luciana Sassone, Roy Stevens
OBJECTIVE: Enterococcus faecalis is a gram-positive facultative anaerobic bacterium, which is present in 30-89% of teeth with postendodontic treatment failures. E. faecalis is capable of penetrating dentinal tubules and surviving as a monoculture after conventional endodontic therapy, indicating that it is resistant to commonly used endodontic disinfection protocols. Different E. faecalis strains have shown resistance to several antibiotics, and have been associated with both dental pathology and systemic infections...
June 12, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28646265/omega-3-and-omega-6-fatty-acids-act-as-inhibitors-of-the-matrix-metalloproteinase-2-and-matrix-metalloproteinase-9-activity
#5
Eleonora Nicolai, Federica Sinibaldi, Gianpaolo Sannino, Giuseppina Laganà, Francesco Basoli, Silvia Licoccia, Paola Cozza, Roberto Santucci, Maria Cristina Piro
Polyunsaturated fatty acids have been reported to play a protective role in a wide range of diseases characterized by an increased metalloproteinases (MMPs) activity. The recent finding that omega-3 and omega-6 fatty acids exert an anti-inflammatory effect in periodontal diseases has stimulated the present study, designed to determine whether such properties derive from a direct inhibitory action of these compounds on the activity of MMPs. To this issue, we investigated the effect exerted by omega-3 and omega-6 fatty acids on the activity of MMP-2 and MMP-9, two enzymes that actively participate to the destruction of the organic matrix of dentin following demineralization operated by bacteria acids...
June 23, 2017: Protein Journal
https://www.readbyqxmd.com/read/28645666/color-masking-measurement-for-ceramic-coating-of-titanium-used-for-dental-implants
#6
Khaldoun Ajlouni, Waleed Elshahawy, Raed Ajlouni, Abdelfattah Sadakah
STATEMENT OF PROBLEM: Poor implant placement or thin gingival tissue and progressive bone resorption may lead to the dark metal color showing through the peri-implant soft tissue. Whether the dark color can be adequately masked is unclear. PURPOSE: The purpose of this in vitro study was to test the color masking effect of porcelain applied on titanium used in fabricating the polished collar of tissue level implants. MATERIAL AND METHODS: Type 4 commercially pure titanium disks and 5 porcelain dentin shades (A2, A3, B2, C2, and D3) were used...
June 20, 2017: Journal of Prosthetic Dentistry
https://www.readbyqxmd.com/read/28643914/the-use-of-cell-culture-platforms-to-identify-novel-markers-of-bone-and-dentin-resorption
#7
W J Rody, O Krokhin, V Spicer, C A Chamberlain, M Chamberlain, K P McHugh, S M Wallet, A K Emory, J D Crull, L S Holliday
OBJECTIVES: 1) To test the hypothesis that there would be proteomic differences in the composition of exosomes isolated from osteoclasts and odontoclasts and 2) to determine the clinical usefulness of these in vitro biomarker candidates. MATERIALS AND METHODS: Mouse bone marrow-derived precursors were cultured on either dentin or bone slices and allowed to mature and begin resorption. Exosomes were isolated from cell culture media and characterized by mass spectrometry...
June 2017: Orthodontics & Craniofacial Research
https://www.readbyqxmd.com/read/28643463/effect-of-ph-on-dentin-protease-inactivation-by-carbodiimide
#8
Roda Seseogullari-Dirihan, Mustafa M Mutluay, Leo Tjäderhane, Lorenzo Breschi, David H Pashley, Arzu Tezvergil-Mutluay
A water-soluble crosslinking agent, 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC), has been used as a pretreatment of acid-etched dentin to inactivate matrix-bound endogenous dentin proteases. The aim of this study was to evaluate the effect of pH on the inactivation capacity of EDC. Demineralized dentin beams (1 × 2 × 6 mm) were divided into six groups (n = 8 per group). Then, EDC (0.3 M) was solubilized in distilled water with pH of 2, 4, 6, 7, 9, or 11. Control EDC was solubilized in 0.1 M 2-(N-morpholino) ethanesulfonic acid (MES) buffer and its pH was adjusted to 6...
June 23, 2017: European Journal of Oral Sciences
https://www.readbyqxmd.com/read/28643450/scpp-genes-and-their-relatives-in-gar-rapid-expansion-of-mineralization-genes-in-osteichthyans
#9
Kazuhiko Kawasaki, Masato Mikami, Mitsushiro Nakatomi, Ingo Braasch, Peter Batzel, John H Postlethwait, Akie Sato, Ichiro Sasagawa, Mikio Ishiyama
Gar is an actinopterygian that has bone, dentin, enameloid, and ganoin (enamel) in teeth and/or scales. Mineralization of these tissues involves genes encoding various secretory calcium-binding phosphoproteins (SCPPs) in osteichthyans, but no SCPP genes have been identified in chondrichthyans to date. In the gar genome, we identified 38 SCPP genes, seven of which encode "acidic-residue-rich" proteins and 31 encode "Pro/Gln (P/Q) rich" proteins. These gar SCPP genes constitute the largest known repertoire, including many newly identified P/Q-rich genes expressed in teeth and/or scales...
June 22, 2017: Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution
https://www.readbyqxmd.com/read/28642929/influence-of-dentin-pretreatment-on-bond-strength-of-universal-adhesives
#10
Claudio Poggio, Riccardo Beltrami, Marco Colombo, Marco Chiesa, Andrea Scribante
Objective: The purpose of the present study was to compare bond strength of different universal adhesives under three different testing conditions: when no pretreatment was applied, after 37% phosphoric acid etching and after glycine application. Materials and methods: One hundred and fifty bovine permanent mandibular incisors were used as a substitute for human teeth. Five different universal adhesives were tested: Futurabond M+, Scotchbond Universal, Clearfil Universal Bond, G-Premio BOND, Peak Universal Bond...
January 2017: Acta Biomaterialia Odontologica Scandinavica
https://www.readbyqxmd.com/read/28642925/the-effect-of-gelatinase-production-of-enterococcus-faecalis-on-adhesion-to-dentin-after-irrigation-with-various-endodontic-irrigants
#11
Mehmet Burak Guneser, Ayce Unverdi Eldeniz
Objectives: The aim of this study was to evaluate whether the gelatinase production ability of Enterococcus faecalis provides any advantage on adhesion of this bacterium to dentin treated with various irrigants and their combinations. Materials and methods: Standardized dentin discs were randomly divided into five groups (n = 20): group 1: 2.5% sodium hypochlorite (NaOCl), group 2: 2% chlorhexidine (CHX), group 3: NaOCl + Saline + CHX, group 4: NaOCl + EDTA + NaOCl, group 5: QMix. After incubation of dentin discs with irrigants, each group was divided into two subgroups (n = 10) according to the bacterial strains used; a gelatinase-producing and a gelatinase-deficient strain of E...
December 2016: Acta Biomaterialia Odontologica Scandinavica
https://www.readbyqxmd.com/read/28642922/universal-cements-dual-activated-and-chemically-activated
#12
Eliane de Lima, Ricardo Santos, Márcia Durão, Armiliana Nascimento, Rodivan Braz
Objective: The aim of the present study was to assess the bond strength of universal cements cured either dually or chemically only. Methods: Three cements were assessed using different types of application: dual activated (DA) or chemically activated (CA). In total 80 dentin blocks were used, obtained through the enamel wear of the lingual and buccal surfaces of bovine incisors. Standard cone-shaped cavity preparations were created using diamond burs. Subsequently, indirect restoration blocks were designed with Filtek Z350 (3M ESPE) composite resin...
December 2016: Acta Biomaterialia Odontologica Scandinavica
https://www.readbyqxmd.com/read/28642915/pre-treating-dentin-with-chlorhexadine-and-cpp-acp-self-etching-and-universal-adhesive-systems
#13
Ricardo Alves Dos Santos, Eliane Alves de Lima, Marcos Antônio Japiassu Resende Montes, Rodivan Braz
Objective: The aim of the present study was to compare the effect of pre-treating dentin with chlorhexidine, at concentrations of 0.2% and 2%, and remineralizing paste containing CPP-ACP (MI Paste - GC) on the bond strength of adhesive systems. Material and methods: In total, 80 slides of dentin were used. These slides were 2 mm thick and were obtained from bovine incisors. Standard cavities were created using diamond bur number 3131. In the control groups, a Scotchbond Universal Adhesive (SUA) self-etching adhesive system of 3M ESPE and a Clearfil SE Bond (CSE) adhesive system of Kuraray were applied, following the manufacturer's instructions...
December 2016: Acta Biomaterialia Odontologica Scandinavica
https://www.readbyqxmd.com/read/28642564/remineralisation-and-arresting-caries-in-children-with-topical-fluorides
#14
Neeraj Gugnani, Shalini Gugnani
Data sourcesThe Cochrane Library, PubMed, Embase and the ISI Web of Science.Study selectionEnglish language clinical trials in children with outcome measures including the remineralisation or arresting effect of caries by professional fluoride treatment were considered.Data extraction and synthesisTwo reviewers screened the studies and assessed risk of bias. Random effects meta-analysis was conducted.ResultsSeventeen studies were included, ten focused on remineralisation, seven on arresting carious lesions...
June 23, 2017: Evidence-based Dentistry
https://www.readbyqxmd.com/read/28641179/quantitative-study-of-the-proportion-of-the-pore-volume-of-human-fluorotic-enamel-filled-by-resin-infiltrant
#15
Frederico Barbosa de Sousa, Isabel Maria Porto Lelis, Regina Célia Bressan Queiroz Figueiredo, Andressa Cavalcanti Pires, Raquel Fernanda Gerlach
AIM: Capillarity theory predicts that the pore volume infiltrated by a liquid in a body with tubular capillaries is directly proportional to the capillary radius. The expected volume available for infiltration is the loosely bound water volume, which can be related to the capillary radii. We tested the hypothesis that the proportion of the pore volume infiltrated by resin infiltrant (Vratio(resin)) is correlated and agrees with the proportion of the pore volume with loosely bound water ( [Formula: see text] )...
June 15, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28638556/effects-of-different-powder-to-liquid-ratios-on-the-push-out-bond-strength-of-cem-cement-on-simulated-perforations-in-the-furcal-area
#16
Mohammad-Frough Reyhani, Negin Ghasemi, Vahid Zand, Saba Mosavizadeh
BACKGROUND: Proper bond strength to dentin is one of the properties of biomaterials used for therapeutic purposes such as repair of furcal perforations. The aim of the present study to evaluate the effects of different powder to liquid ratios of Calcium-enriched mixture (CEM) on the push-out bond strengths in simulated perforations in the furcal area and compare it with Mineral trioxide aggregate (MTA). MATERIAL AND METHODS: Furcal perforations, measuring 1.3 mm in diameter and 2 mm in height, were prepared in 120 mandibular first molars...
June 2017: Journal of Clinical and Experimental Dentistry
https://www.readbyqxmd.com/read/28638548/dentin-bonding-agent-with-improved-bond-strength-to-dentin-through-incorporation-of-sepiolite-nanoparticles
#17
Farnoosh Fallahzadeh, Shadab Safarzadeh-Khosroshahi, Mohammad Atai
BACKGROUND: The study aims to investigate the effect of incorporation of sepiolite nanoparticles on the microtensile bond strength of an experimental dentin bonding to the human dentin. MATERIAL AND METHODS: The sepiolite nanoparticles were incorporated into an experimental methacrylate-based dentin bonding system in concentrations of 0.0, 0.2, 1.0, 2.0, and 5.0 weight percents. The specimens were then ultrasonicated to finely disperse the nanoparticles in the adhesive matrix...
June 2017: Journal of Clinical and Experimental Dentistry
https://www.readbyqxmd.com/read/28638547/push-out-bond-strength-of-different-endodontic-obturation-material-at-three-different-sites-in-vitro-study
#18
Pankaj Mishra, Anjna Sharma, Sunil Mishra, Manas Gupta
BACKGROUND: The key to success of any root canal therapy is adequate obturation of the prepared root canal space. Root canal sealers are not dimensionally stable and might dissolve partially over a period of time. The objective of this in vitro study is to evaluate the push-out bond strength to intraradicular dentin of two endodontic obturation materials. MATERIAL AND METHODS: Forty extracted single rooted permanent teeth were used. Canals orifice was explored, teeth were instrumented...
June 2017: Journal of Clinical and Experimental Dentistry
https://www.readbyqxmd.com/read/28638345/heterogeneity-and-developmental-connections-between-cell-types-inhabiting-teeth
#19
REVIEW
Jan Krivanek, Igor Adameyko, Kaj Fried
Every tissue is composed of multiple cell types that are developmentally, evolutionary and functionally integrated into the unit we call an organ. Teeth, our organs for biting and mastication, are complex and made of many different cell types connected or disconnected in terms of their ontogeny. In general, epithelial and mesenchymal compartments represent the major framework of tooth formation. Thus, they give rise to the two most important matrix-producing populations: ameloblasts generating enamel and odontoblasts producing dentin...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28636235/stem-cell-based-tooth-and-periodontal-regeneration
#20
Lei Hu, Yi Liu, Songlin Wang
Currently regeneration of tooth and periodontal damage still remains great challenge. Stem cell-based tissue engineering raised novel therapeutic strategies for tooth and periodontal repair. Stem cells for tooth and periodontal regeneration include dental pulp stem cells (DPSCs), periodontal ligament stem cells (PDLSCs), stem cells from the dental apical papilla (SCAPs), and stem cells from human exfoliated deciduous teeth (SHEDs), dental follicle stem cells (DFSCs), dental epithelial stem cells (DESCs), bone marrow mesenchymal stem cells (BMMSCs), adipose-derived stem cells (ADSCs), embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs)...
June 21, 2017: Oral Diseases
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