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https://www.readbyqxmd.com/read/29151196/oral-cancer-radiotherapy-affects-enamel-microhardness-and-associated-indentation-pattern-morphology
#1
R Seyedmahmoud, Y Wang, G Thiagarajan, J P Gorski, R Reed Edwards, J D McGuire, M P Walker
OBJECTIVES: The aim of this study is to determine the effects of in vitro and in vivo high-dose radiotherapy on microhardness and associated indentation pattern morphology of enamel. MATERIALS AND METHODS: The inner, middle, and outer microhardness of enamel was evaluated using three experimental groups: control (non-radiated); in vitro irradiated; in vivo irradiated. In vitro specimens were exposed to simulated radiotherapy, and in vivo specimens were extracted teeth from oral cancer patients previously treated with radiotherapy...
November 18, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/29150134/stress-generation-in-mandibular-anterior-teeth-restored-with-different-types-of-post-and-core-at-various-levels-of-ferrule
#2
Anatolii Savychuk, Marianthi Manda, Christos Galanis, Christofer Provatidis, Petros Koidis
STATEMENT OF PROBLEM: Pertinent evidence regarding the mechanical integrity of mandibular anterior teeth restored with a post-and-core is limited. PURPOSE: The purpose of this finite element analysis study was to compare the impact of the post type (glass fiber post-and-resin core or cast post-and-core) along with the ferrule effect on the stress fields generated in endodontically treated mandibular lateral incisors and canines. MATERIAL AND METHODS: Three-dimensional models of the segmented mandible were developed...
November 15, 2017: Journal of Prosthetic Dentistry
https://www.readbyqxmd.com/read/29148982/a-dentin-derived-hydrogel-bioink-for-3d-printing-of-cell-laden-scaffolds-for-regenerative-dentistry
#3
Avathamsa Athirasala, Anthony Tahayeri, Greeshma Thrivikraman, Cristiane Miranda Franca, Nelson Monteiro, Victor Tran, Jack Ferracane, Luiz Bertassoni
Recent studies in tissue engineering have adopted extracellular matrix (ECM) derived scaffolds as natural and cytocompatible microenvironments for tissue regeneration. The dentin matrix, specifically, has been shown to be associated with a host of soluble and insoluble signaling molecules that can promote odontogenesis. Here, we have developed a novel bioink, blending printable alginate (3% w/v) hydrogels with the soluble and insoluble fractions of the dentin matrix. We have optimized the printing parameters and the concentrations of the individual components of the bioink for print accuracy, cell viability and odontogenic potential...
November 17, 2017: Biofabrication
https://www.readbyqxmd.com/read/29148914/marginal-gap-formation-in-approximal-bulk-fill-resin-composite-restorations-after-artificial-ageing
#4
A Peutzfeldt, S Mühlebach, A Lussi, S Flury
The aim of this in vitro study was to investigate the marginal gap formation of a packable "regular" resin composite (Filtek Supreme XTE [3M ESPE]) and two flowable "bulk fill" resin composites (Filtek Bulk Fill [3M ESPE] and SDR [DENTSPLY DeTrey]) along the approximal margins of Class II restorations. In each of 39 extracted human molars (n=13 per resin composite), mesial and distal Class II cavities were prepared, placing the gingival margins below the cemento-enamel junction. The cavities were restored with the adhesive system OptiBond FL (Kerr) and one of the three resin composites...
November 17, 2017: Operative Dentistry
https://www.readbyqxmd.com/read/29146302/mechanisms-involved-in-bone-resorption-regulated-by-vitamin-d
#5
REVIEW
Yuko Nakamichi, Nobuyuki Udagawa, Tatsuo Suda, Naoyuki Takahashi
Active forms of vitamin D enhance osteoclastogenesis in vitro and in vivo through the vitamin D receptor (VDR) in osteoblast-lineage cells consisting of osteoblasts and osteocytes. This pro-resorptive activity was evident basically with higher concentrations of active vitamin D than those expected in physiological conditions. Nevertheless, vitamin D compounds have been used in Japan for treating osteoporosis to increase bone mineral density (BMD). Of note, the increase in BMD by long-term treatment with pharmacological (=near-physiological) doses of vitamin D compounds was caused by the suppression of bone resorption...
November 13, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29146046/influence-of-different-smear-layers-on-bond-durability-of-self-etch-adhesives
#6
Toshiki Takamizawa, Wayne W Barkmeier, Keiichi Sai, Akimasa Tsujimoto, Arisa Imai, Robert L Erickson, Mark A Latta, Masashi Miyazaki
OBJECTIVE: The purpose of this study was to determine the influence of different smear layers on enamel and dentin bond durability of various types of self-etch adhesives. METHODS: Two universal adhesives, Scotchbond Universal (SU) and Prime & Bond elect (PE); a conventional single-step self-etch adhesive, G-ænial Bond (GB); and two two-step self-etch adhesives, Optibond XTR (OX) and Clearfil SE Bond (SE) were used in this study. Shear bond strengths (SBS) and shear fatigue strengths (SFS) to human enamel and dentin were determined with different smear layer conditions...
November 13, 2017: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/29144877/role-of-proteolytic-enzyme-inhibitors-on-carious-and-eroded-dentin-associated-with-a-universal-bonding-system
#7
M C Giacomini, Pmc Scaffa, L P Chaves, Cmp Vidal, T N Machado, H M Honório, L Tjäderhane, L Wang
OBJECTIVES: The aim of this study was to evaluate the effect of proteolytic inhibitors on the bond strength of a universal adhesive system (etch-and-rinse mode) applied to artificial carious and eroded dentin. METHODS: Ninety molars were prepared and randomly divided into three groups according to the substrate: N, no challenges; ACD, artificial carious dentin simulation and ERO, artificial erosion simulation with orange juice. All groups were redivided into three subgroups according to the dentin pretreatment: W, water; CHX, 2% digluconate chlorhexidine; and E-64 (trans-epoxysuccinyl-L-leucylamido-[4-guanidino] butane), 5 μM E-64 inhibitor...
November 2017: Operative Dentistry
https://www.readbyqxmd.com/read/29142882/incidence-of-apical-crack-formation-and-propagation-during-removal-of-root-canal-filling-materials-with-different-engine-driven-nickel-titanium-instruments
#8
Taha Özyürek, Vildan Tek, Koray Yılmaz, Gülşah Uslu
Objectives: To determine the incidence of crack formation and propagation in apical root dentin after retreatment procedures performed using ProTaper Universal Retreatment (PTR), Mtwo-R, ProTaper Next (PTN), and Twisted File Adaptive (TFA) systems. Materials and Methods: The study consisted of 120 extracted mandibular premolars. One millimeter from the apex of each tooth was ground perpendicular to the long axis of the tooth, and the apical surface was polished...
November 2017: Restorative Dentistry & Endodontics
https://www.readbyqxmd.com/read/29140093/early-diagnosis-and-treatment-of-asymptomatic-vertical-enamel-and-dentin-cracks
#9
Samer S Alassaad
Asymptomatic vertical enamel and dentin cracks can lead to multiple pathologic consequences ranging from caries to pulpal involvement, periodontal involvement, complete tooth fracture, and tooth loss if intervention is postponed. Early diagnosis, using magnification, tactile examination, transillumination, and dyes, and early restorative and occlusal treatments can be incorporated into daily practice to prevent further crack propagation before asymptomatic cracks become symptomatic and cause further damage to the dentition and surrounding tissues...
November 2017: Compendium of Continuing Education in Dentistry
https://www.readbyqxmd.com/read/29138868/hdac-inhibitor-lmk%C3%A2-235-promotes-the-odontoblast-differentiation-of-dental-pulp-cells
#10
Zhao Liu, Ting Chen, Qianqian Han, Ming Chen, Jie You, Fuchun Fang, Ling Peng, Buling Wu
The role of dental pulp cells (DPCs) in hard dental tissue regeneration had received increasing attention because DPCs can differentiate into odontoblasts and other tissue‑specific cells. In recent years, epigenetic modifications had been identified to serve an important role in cell differentiation, and histone deacetylase (HDAC) inhibitors have been widely studied by many researchers. However, the effects of HDAC4 and HDAC5 on the differentiation of DPCs and the precise molecular mechanisms remain unclear...
November 14, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29138798/hey1-functions-as-a-positive-regulator-of-odontogenic-differentiation-in-odontoblast%C3%A2-lineage-cells
#11
Xiao Yin, Zhaobin Zeng, Jinliang Xing, Ansheng Zhang, Wenkai Jiang, Wei Wang, Hantang Sun, Longxing Ni
Substantial evidence has indicated that Notch and bone morphogenetic protein (BMP) signaling may regulate odontoblastic differentiation. Hairy/enhancer‑of‑split related with YRPW motif 1 (Hey1), a downstream target gene of Notch and BMP signaling, is expressed in dental pulp tissues and has been demonstrated to be responsible for osteoblast mineralization. The aim of this study was to investigate the effects of Hey1 on odontoblast differentiation. The results of the study demonstrated that Hey1 expression in odontoblast‑lineage cells (OLCs) was upregulated by stimulation of osteoblastic/odontoblastic differentiation medium containing ascorbic acid, β‑glycerol phosphate and dexamethasone...
November 13, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29138112/dentists-decision-strategies-for-suspicious-occlusal-caries-lesions-in-a-national-dental-pbrn-study
#12
Sonia K Makhija, Michael E Robinson, James D Bader, Daniel A Shugars, Mark S Litaker, Hong R Im, D Brad Rindal, Daniel J Pihlstrom, Cyril Meyerowitz, Valeria V Gordan, Meredith K Buchberg, Gregg H Gilbert
OBJECTIVE: Case presentations (vignettes) were completed by dentists in the National Dental Practice-Based Research Network study "Decision Aids for the Management of Suspicious Occlusal Caries Lesions (SOCLs)". The objective was to determine dentists' decision strategies for SOCLs). METHODS: 107 dentists viewed a series of 16 vignettes that represented all combinations of 4 clinical cues: color, luster, lesion roughness, and patient-level caries risk. Each vignette included a patient description and a photograph of a tooth presenting the 4 cues...
November 11, 2017: Journal of Dentistry
https://www.readbyqxmd.com/read/29137807/mapping-the-mechanical-gradient-of-human-dentin-enamel-junction-at-different-intratooth-locations
#13
Zhengzhi Wang, Kun Wang, Wanyin Xu, Xiaoyu Gong, Feiyu Zhang
OBJECTIVES: The local structures and mechanical properties within tooth dentin-enamel-junction (DEJ) regions have been focused for numerous studies. The reported results, however, remain inconsistent particularly on the functional width and gradient architecture of the DEJ. The current study aims at systematically determining the mechanical gradient of the DEJ at different intratooth locations such that a clearer understanding on the tooth properties and the potential correlations with the tooth function could be obtained...
November 11, 2017: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/29135948/effect-of-a-lactobacillus-salivarius-probiotic-on-a-double-species-streptococcus-mutans-and-candida-albicans-caries-biofilm
#14
Wirginia Krzyściak, Dorota Kościelniak, Monika Papież, Palina Vyhouskaya, Katarzyna Zagórska-Świeży, Iwona Kołodziej, Beata Bystrowska, Anna Jurczak
The aim of the study was to evaluate the anti-cariogenic effects of Lactobacillus salivarius by reducing pathogenic species and biofilm mass in a double-species biofilm model. Coexistence of S. mutans with C. albicans can cause dental caries progression or recurrence of the disease in the future. Fifty-nine children with diagnosed early childhood caries (ECC) were recruited onto the study. The condition of the children's dentition was defined according to the World Health Organization guidelines. The participants were divided into children with initial enamel demineralization and children showing dentin damage...
November 14, 2017: Nutrients
https://www.readbyqxmd.com/read/29133967/comparison-of-marginal-microleakage-of-flowable-composite-restorations-in-primary-canine-teeth-prepared-with-high-speed-diamond-bur-er-yag-laser-and-er-cr-ysgg-laser
#15
Beheshteh Malekafzali, Mohammad Asnaashari, Fateme Javadi
Background and Objectives: Adhesive restorations have become highly popular in pediatric dentistry, and novel methods of cavity preparation with minimal patient discomfort including Er:YAG and Er,Cr:YSGG laser preparations have greatly advanced. This study aimed to compare the microleakage of composite restorations of class V cavities prepared in primary canine teeth with high-speed bur and Er:YAG and Er,Cr:YSGG lasers. Materials and Methods: This in vitro study was conducted on 30 class V cavities prepared on the buccal surfaces of primary canine teeth in three groups (n = 10) of bur preparation, Er:YAG laser and Er,Cr:YSGG laser...
September 30, 2017: Laser Therapy
https://www.readbyqxmd.com/read/29130564/effect-of-non-thermal-atmospheric-plasma-on-the-dentin-surface-topography-and-composition-and-on-the-bond-strength-of-a-universal-adhesive
#16
Ana P Ayres, Jean J Bonvent, Borys Mogilevych, Luis E S Soares, Airton A Martin, Glaucia M Ambrosano, Fábio D Nascimento, Bart Van Meerbeek, Marcelo Giannini
This study investigated the effect of application of non-thermal atmospheric plasma (NTAP) on the topography and composition of the dentin surface, as well as the microtensile bond strength (μTBS) of a universal adhesive to NTAP-treated dentin. Exposed flat dentin surfaces from human third molars were either treated with NTAP for 10 and 30 s or untreated (control). The dentin-surface topography and chemical composition were characterized by atomic force microscopy (n = 3) and Raman confocal spectroscopy (n = 5), respectively...
November 11, 2017: European Journal of Oral Sciences
https://www.readbyqxmd.com/read/29130364/fluorescent-labeling-and-2-photon-imaging-of-mouse-tooth-pulp-nociceptors
#17
A Kadala, P Sotelo-Hitschfeld, Z Ahmad, P Tripal, B Schmid, A Mueller, L Bernal, Z Winter, S Brauchi, U Lohbauer, K Messlinger, J K Lennerz, K Zimmermann
Retrograde fluorescent labeling of dental primary afferent neurons (DPANs) has been described in rats through crystalline fluorescent DiI, while in the mouse, this technique was achieved with only Fluoro-Gold, a neurotoxic fluorescent dye with membrane penetration characteristics superior to the carbocyanine dyes. We reevaluated this technique in the rat with the aim to transfer it to the mouse because comprehensive physiologic studies require access to the mouse as a model organism. Using conventional immunohistochemistry, we assessed in rats and mice the speed of axonal dye transport from the application site to the trigeminal ganglion, the numbers of stained DPANs, and the fluorescence intensity via 1) conventional crystalline DiI and 2) a novel DiI formulation with improved penetration properties and staining efficiency...
November 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/29128128/cad-fe-modeling-and-analysis-of-class-ii-restorations-incorporating-resin-composite-glass-ionomer-and-glass-ceramic-materials
#18
Pietro Ausiello, Stefano Ciaramella, Massimo Martorelli, Antonio Lanzotti, Antonio Gloria, David C Watts
OBJECTIVES: To investigate the influence of specific resin-composite, glass ceramic and glass ionomer cement (GIC) material combinations in a "multi-layer" technique to replace enamel and dentin in class II mesio-occlusal-distal (MOD) dental restorations using 3D-Finite Element Analysis (FEA). METHODS: Four 3D-FE models (A-D) of teeth, adhesively restored with different filling materials, were created and analyzed in comparison with a 3D model (E) of a sound lower molar...
November 8, 2017: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/29126633/mechanical-behavior-of-endodontically-restored-canine-teeth-effects-of-ferrule-post-material-and-shape
#19
Pietro Ausiello, Stefano Ciaramella, Massimo Martorelli, Antonio Lanzotti, Fernando Zarone, David C Watts, Antonio Gloria
OBJECTIVE: To assess the effect of a ferrule design with specific post material-shape combinations on the mechanical behavior of post-restored canine teeth. METHODS: Micro-CT scan images of an intact canine were used to create a 3-D tessellated CAD model, from which the shapes of dentin, pulp and enamel were obtained and geometric models of post-endodontically restored teeth were created. Two types of 15mm post were evaluated: a quartz fiber post with conical-tapered shape, and a carbon (C) fiber post with conical-cylindrical shape...
November 7, 2017: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/29126370/evaluation-of-final-irrigation-regimens-with-maleic-acid-for-smear-layer-removal-and-wettability-of-root-canal-sealer
#20
Nidambur Vasudev Ballal, Carmen Maria Ferrer-Luque, Mrunali Sona, K Narayan Prabhu, Teresa Arias-Moliz, Pilar Baca
OBJECTIVE: To evaluate the smear layer removal and wettability of AH Plus sealer on root canal dentin treated with MA (maleic acid), MA + CTR (cetrimide) and MA + CTR + CHX (chlorhexidine) as final irrigating regimens. MATERIAL AND METHODS: For smear layer removal, 40 teeth were instrumented to size F4 and divided into four groups: (1) 7% MA, (2) 7% MA + 0.2% CTR, (3) 7% MA + 0.2% CTR + 2% CHX, (4) distilled water (control). After irrigation, teeth were subjected to SEM analysis...
November 10, 2017: Acta Odontologica Scandinavica
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