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https://www.readbyqxmd.com/read/28346710/the-arabidopsis-thiamin-deficient-mutant-pale-green1-lacks-thiamin-monophosphate-phosphatase-of-the-vitamin-b1-biosynthesis-pathway
#1
Wei-Yu Hsieh, Jo-Chien Liao, Hsin-Tzu Wang, Tzu-Huan Hung, Ching-Chih Tseng, Tsui-Yun Chung, Ming-Hsiun Hsieh
Thiamin diphosphate (TPP, vitamin B1) is an essential coenzyme present in all organisms. Animals obtain TPP from their diets, but plants synthesize TPP de novo. We isolated and characterized an Arabidopsis pale green1 (pale1) mutant that contained higher concentrations of thiamin monophosphate (TMP) and less thiamin and TPP than the wild type. Supplementation with thiamin, but not the thiazole and pyrimidine precursors, rescued the mutant phenotype, indicating that the pale1 mutant is a thiamin-deficient mutant...
March 27, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28346351/in-vitro-and-in-vivo-studies-on-the-structural-organization-of-chs3-from-saccharomyces-cerevisiae
#2
Simon Gohlke, Subbaratnam Muthukrishnan, Hans Merzendorfer
Chitin biosynthesis in yeast is accomplished by three chitin synthases (Chs) termed Chs1, Chs2 and Chs3, of which the latter accounts for most of the chitin deposited within the cell wall. While the overall structures of Chs1 and Chs2 are similar to those of other chitin synthases from fungi and arthropods, Chs3 lacks some of the C-terminal transmembrane helices raising questions regarding its structure and topology. To fill this gap of knowledge, we performed bioinformatic analyses and protease protection assays that revealed significant information about the catalytic domain, the chitin-translocating channel and the interfacial helices in between...
March 25, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28346061/understanding-the-contamination-of-food-with-mineral-oil-the-need-for-a-confirmatory-analytical-and-procedural-approach
#3
Lionel W Spack, Gabriela Leszczyk, Jesus Varela, Hervé Simian, Thomas Gude, Richard H Stadler
The contamination of food by mineral oil hydrocarbons (MOH) found in packaging is a long running concern. A main source of MOH in foods is the migration of mineral oil from recycled board into the packed food products. Consequently, the majority of food manufacturers have taken protective measures, for example by using virgin board instead of recycled fibers, and where feasible, introducing functional barriers to mitigate migration. Despite these protective measures, MOH may still be observed at low amounts in certain food products, albeit due to different entry points across the food supply chain...
March 27, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28345968/relationship-between-phospholipase-c-zeta-semen-parameters-and-chromatin-status
#4
Marziyeh Tavalaee, Abbas Kiani-Esfahani, Mohammad H Nasr-Esfahani
The need for additional tests to complement basic sperm analysis in clinics is well appreciated. In this regard, a number of tests such as sperm DNA integrity test as a tool in diagnosis and treatment of infertility are suggested. But recent studies have focused on main sperm factors involved in oocyte activation such as phospholipase C-zeta (PLCζ) that initiate intracellular Ca(2+) signaling and embryogenesis. Therefore, this study aimed to investigate the relationship between PLCζ, basic semen parameters, sperm DNA fragmentation (SDF), and protamine deficiency in men with normal (n=32) and abnormal (n=23) semen parameters...
March 27, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28345933/structure-energy-relationships-of-halogen-bonds-in-proteins
#5
Matthew R Scholfield, Melissa Coates Ford, Anna-Carin Cecilia Carlsson, Hawera Butta, Ryan A Mehl, Pui Shing Ho
The structures and stabilities of proteins are defined by a series of weak non-covalent electrostatic, van der Waals, and hydrogen bond (HB) interactions. In this study, we have designed and engineered halogen bonds (XBs) site-specifically in order to study their structure-energy relationship in a model protein, T4 lysozyme. The evidence for XBs is the displacement of the aromatic side chain towards an oxygen acceptor, at distances that are at or less than the sums of their respective van der Waals radii, when the hydroxyl substituent of the wildtype tyrosine is replaced by an iodine...
March 27, 2017: Biochemistry
https://www.readbyqxmd.com/read/28345929/utility-of-cyp3a4-and-pxr-car-cyp3a4-3a7-transgenic-mouse-models-to-assess-the-magnitude-of-cyp3a4-mediated-drug-drug-interactions
#6
Justin Q Ly, Kirsten Messick, Ann Qin, Ryan H Takahashi, Edna F Choo
Species differences in the expression, activity, regulation and substrate specificity of metabolizing enzymes preclude the use of animal models to predict clinical drug-drug interactions (DDI). The objective of this work is to determine if the transgenic (Tg) Cyp3a-/-Tg-3A4Hep/Int and Nr1i2/Nr1i3-/--Cyp3a-/-Tg-PXR-CAR-3A4/3A7Hep/Int (PXR-CAR-CYP3A4/3A7) mouse models could be used to predict in vivo DDI of 10 drugs; alprazolam, bosutinib, crizotinib, dasatinib, gefitinib, ibrutinib, regorafenib, sorafenib, triazolam, and vandetinib (as victims), with varying magnitudes of reported CYP3A4 clinical DDI...
March 27, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28345844/association-between-the-dna-repair-gene-xrcc3-rs861539-polymorphism-and-risk-of-osteosarcoma-a-systematic-review-and-meta-analysis
#7
Mohammad Reza Sobhan, Mohammad Forat Yazdi, Mahta Mazaheri, Masoud Zare Shehneh, Hossein Neamatzadeh
Objective: Although there are a few studies investigating the relation between X-Ray Repair Cross Complementing 3 (XRCC3) gene rs861539 polymorphism and osteosarcoma (OSA), the results are inconsistent. Therefore, we performed this systematic review and meta-analysis to clarify the associations between XRCC3 rs861539 polymorphism and OSA risk. Methods: We have retrieved published literature from PubMed, Google scholar, and ISI Web of Knowledge up to 25 January 2017. Odds ratios were pooled using either fixed-effects or random effects models...
February 1, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28345838/ercc1-expression-in-metastatic-triple-negative-breast-cancer-patients-treated-with-platinum-based-chemotherapy
#8
Mohamed Ali EL Baiomy, Wagdi F El Kashef
Background: Possible targeted therapies for metastatic triple negative breast cancer (TNBC) include cytotoxic chemotherapy that causes interstrand breaks (platinum-based drugs). The excision repair cross-complementation 1 (ERCC1) enzyme plays an essential role in the nucleotide excision repair pathway, removing platinum-induced DNA adducts and contributing to cisplatin resistance. Detecting ERCC1 overexpression is important in considering treatment options for metastatic TNBC, including individualized approaches to therapy, and may facilitate improved responses or reduction of unnecessary toxicity...
February 1, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28345712/metal-free-phosphonation-of-heteroarene-n-oxides-with-trialkyl-phosphite-at-room-temperature
#9
Ming-Tao Chen, Xia You, Li-Gang Bai, Qun-Li Luo
A new protocol is described for the conversion of heteroarene N-oxides to heteroarylphosphonates through in situ activation with bromotrichloromethane. The N-oxides of isoquinoline, quinoline, quinoxaline and 1,10-phenanthroline were fast transformed into the corresponding heteroarylphosphonates in up to 92% yield under mild conditions in the absence of solvent and metal catalysts. The good functional group tolerance, low cost, feasibility of scale up, and wide availability of reagents make this method a prominent complement to the Hirao coupling...
March 27, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28345510/a-cephalometric-study-to-establish-the-relationship-of-the-occlusal-plane-to-the-three-different-ala-tragal-lines-and-the-frankfort-horizontal-plane-in-different-head-forms
#10
S Subhas, P L Rupesh, M Chadda, D R V Kumar, R Prasad, N Kumari
BACKGROUND: The aim of the study is to compare the relationship of the occlusal plane to 3 different ala-tragal lines, namely the superior, middle and inferior lines, in individuals having different head forms and its relation to the Frankfort horizontal plane. METHODOLOGY: A total of 75 lateral cephalometric radiographs of subjects with natural dentition, having full complement of teeth, between the age group of 18-25 were screened and selected. Lateral cephalogram were made for each subjects in an open mouth position...
March 17, 2017: J Stomatol Oral Maxillofac Surg
https://www.readbyqxmd.com/read/28345458/proteasome-expression-and-activity-in-cancer-and-cancer-stem-cells
#11
Ioannis A Voutsadakis
Proteasome is a multi-protein organelle that participates in cellular proteostasis by destroying damaged or short-lived proteins in an organized manner guided by the ubiquitination signal. By being in a central place in the cellular protein complement homeostasis, proteasome is involved in virtually all cell processes including decisions on cell survival or death, cell cycle, and differentiation. These processes are important also in cancer, and thus, the proteasome is an important regulator of carcinogenesis...
March 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28345293/detection-of-zaire-ebolavirus-in-swine-assay-development-and-optimization
#12
B S Pickering, B Collignon, G Smith, P Marszal, G Kobinger, H M Weingartl
Ebolaviruses (family Filoviridae, order Mononegavirales) cause often fatal, haemorrhagic fever in primates including humans. Pigs have been identified as a species susceptible to Reston ebolavirus (RESTV) infection, with indicated transmission to humans in the Philippines; however, their role during Ebola outbreaks in Africa needs to be clarified. To perform surveillance studies, detection of ebolavirus requires a prerequisite validation of viral RNA and antibody detection methods in swine samples. These diagnostic tests also need to be suitable for deployment to low-level containment laboratories...
March 27, 2017: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/28345261/antibodies-from-plants-for-bionanomaterials
#13
REVIEW
Gueven Edgue, Richard M Twyman, Veronique Beiss, Rainer Fischer, Markus Sack
Antibodies are produced as part of the vertebrate adaptive immune response and are not naturally made by plants. However, antibody DNA sequences can be introduced into plants, and together with laboratory technologies that allow the design of antibodies recognizing any conceivable molecular structure, plants can be used as 'green factories' to produce any antibody at all. The advent of plant-based transient expression systems in particular allows the rapid, convenient, and safe production of antibodies, ranging from laboratory-scale expression to industrial-scale manufacturing...
March 27, 2017: Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology
https://www.readbyqxmd.com/read/28345259/the-role-of-complement-inhibitors-beyond-controlling-inflammation
#14
REVIEW
A M Blom
The complement system is an arm of innate immunity that aids in the removal of pathogens and dying cells. Due to its harmful, pro-inflammatory potential, complement is controlled by several soluble and membrane-bound inhibitors. This family of complement regulators has been recently extended by the discovery of several new members, and it is becoming apparent that these proteins harbour additional functions. In this review, the current state of knowledge of the physiological functions of four complement regulators will be described: cartilage oligomeric matrix protein (COMP), CUB and sushi multiple domains 1 (CSMD1), sushi domain-containing protein 4 (SUSD4) and CD59...
March 26, 2017: Journal of Internal Medicine
https://www.readbyqxmd.com/read/28344842/effectiveness-of-direct-safety-regulations-on-manufacturers-and-users-of-industrial-machines-its-implications-on-industrial-safety-policies-in-republic-of-korea
#15
Gi Heung Choi
BACKGROUND: Despite considerable efforts made in recent years, the industrial accident rate and the fatality rate in the Republic of Korea are much higher than those in most developed countries in Europe and North America. Industrial safety policies and safety regulations are also known to be ineffective and inefficient in some cases. METHODS: This study focuses on the quantitative evaluation of the effectiveness of direct safety regulations such as safety certification, self-declaration of conformity, and safety inspection of industrial machines in the Republic of Korea...
March 2017: Safety and Health At Work
https://www.readbyqxmd.com/read/28344632/dynamic-and-temporal-assessment-of-human-dried-blood-spot-ms-ms-all-shotgun-lipidomics-analysis
#16
Fei Gao, Justice McDaniel, Emily Y Chen, Hannah E Rockwell, Jeremy Drolet, Vivek K Vishnudas, Vladimir Tolstikov, Rangaprasad Sarangarajan, Niven R Narain, Michael A Kiebish
BACKGROUND: Real-time and dynamic assessment of an individual's lipid homeostatic state in blood is complicated due to the need to collect samples in a clinical environment. In the context of precision medicine and population health, tools that facilitate sample collection and empower the individual to participate in the process are necessary to complement advanced bioanalytical analysis. The dried blood spot (DBS) methodology via finger prick or heel prick is a minimally invasive sample collection method that allows the relative ease and low cost of sample collection as well as transport...
2017: Nutrition & Metabolism
https://www.readbyqxmd.com/read/28344595/alpha-type-phospholipase-a2-inhibitors-from-snake-blood
#17
REVIEW
Norival A Santos-Filho, Claudia T Santos
It is of popular and scientific knowledge that toxins from snake venom (among them the PLA2 and myotoxins) are neutralized by various compounds, such as antibodies and proteins purified from animal blood. Venomous and nonvenomous snakes have PLA2 inhibitory proteins, called PLIs, in their blood serum. One hypothesis that could explain the presence of these PLIs in the serum of venomous snakes would be self-protection against the enzymes of their own venom, which eventually could reach the circulatory system...
2017: Journal of Venomous Animals and Toxins Including Tropical Diseases
https://www.readbyqxmd.com/read/28344571/translation-initiation-factor-eif4e-and-eifiso4e-are-both-required-for-peanut-stripe-virus-infection-in-peanut-arachis-hypogaea-l
#18
Manlin Xu, Hongfeng Xie, Juxiang Wu, Lianhui Xie, Jinguang Yang, Yucheng Chi
Peanut stripe virus (PStV) belongs to the genus Potyvirus and is the most important viral pathogen of cultivated peanut (Arachis hypogaea L.). The eukaryotic translation initiation factor, eIF4E, and its isoform, eIF(iso)4E, play key roles during virus infection in plants, particularly Potyvirus. In the present study, we cloned the eIF4E and eIF(iso)4E homologs in peanut and named these as PeaeIF4E and PeaeIF(iso)4E, respectively. Quantitative real-time PCR (qRT-PCR) analysis showed that these two genes were expressed during all growth periods and in all peanut organs, but were especially abundant in young leaves and roots...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28344517/economic-evaluation-of-participatory-learning-and-action-with-women-s-groups-facilitated-by-accredited-social-health-activists-to-improve-birth-outcomes-in-rural-eastern-india
#19
Rajesh Kumar Sinha, Hassan Haghparast-Bidgoli, Prasanta Kishore Tripathy, Nirmala Nair, Rajkumar Gope, Shibanand Rath, Audrey Prost
BACKGROUND: Neonatal mortality remains unacceptably high in many low and middle-income countries, including India. A community mobilisation intervention using participatory learning and action with women's groups facilitated by Accredited Social Health Activists (ASHAs) was conducted to improve maternal and newborn health. The intervention was evaluated through a cluster-randomised controlled trial conducted in Jharkhand and Odisha, eastern India. This aims to assess the cost-effectiveness this intervention...
2017: Cost Effectiveness and Resource Allocation: C/E
https://www.readbyqxmd.com/read/28344191/transposition-of-insertion-sequence-is256bsu1-in-bacillus-subtilis-168-is-strictly-dependent-on-reca
#20
Motohiro Akashi, Shota Harada, Syunsuke Moki, Yuki Okouji, Kiwamu Takahashi, Shigeki Kada, Keigo Yamagami, Yasuhiko Sekine, Satoru Watanabe, Taku Chibazakura, Hirofumi Yoshikawa
We developed an insertion sequence transposition detection system called the "jumping cat assay" and applied it to the Bacillus subtilis chromosome using IS256Bsu1 derived from B. subtilis natto. The high frequency of transposition enabled us to explore host factors; combining the assay and genetic analyses revealed that recA is essential for the transposition of IS256Bsu1. Detailed analyses using various domain mutants of recA demonstrated that this essentiality is not related to the function of recA in homologous recombination...
March 24, 2017: Genes & Genetic Systems
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