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Hong Ling, Xu Zeng, Shunxing Guo
Late embryogenesis abundant (LEA) proteins, a diverse family, accumulate during seed desiccation in the later stages of embryogenesis. LEA proteins are associated with tolerance to abiotic stresses, such as drought, salinity and high or cold temperature. Here, we report the first comprehensive survey of the LEA gene family in Dendrobium officinale, an important and widely grown medicinal orchid in China. Based on phylogenetic relationships with the complete set of Arabidopsis and Oryza LEA proteins, 17 genes encoding D...
December 22, 2016: Scientific Reports
Jing Nie, Yong Tian, Yu Zhang, Yan-Liu Lu, Li-Sheng Li, Jing-Shan Shi
BACKGROUND: Neuronal and synaptic loss is the most important risk factor for cognitive impairment. Inhibiting neuronal apoptosis and preventing synaptic loss are promising therapeutic approaches for Alzheimer's disease (AD). In this study, we investigate the protective effects of Dendrobium alkaloids (DNLA), a Chinese medicinal herb extract, on β-amyloid peptide segment 25-35 (Aβ25-35)-induced neuron and synaptic loss in mice. METHOD: Aβ25-35(10 µg) was injected into the bilateral ventricles of male mice followed by an oral administration of DNLA (40 mg/kg) for 19 days...
2016: PeerJ
Chunmei He, Songjun Zeng, Jaime A Teixeira da Silva, Zhenming Yu, Jianwen Tan, Jun Duan
Phosphomannomutase (PMM, EC catalyzes the interconversion of mannose-6-phosphate to mannose-1-phosphate, the precursor for the synthesis of GDP-mannose. In this study, the complementary DNA (cDNA) of the Phosphomannomutase (PMM) gene was initially cloned from Dendrobium officinale by RACE method. Transient transform result showed that the DoPMM protein was localized in the cytoplasm. The DoPMM gene was highly expressed in the stems of D. officinale both in vegetative and reproductive developmental stages...
December 16, 2016: Protoplasma
W Wei, L Feng, S P Nie, Q B Han
No abstract text is available yet for this article.
December 2016: Planta Medica
Huanhuan Chen, Yuechun Huang, Junbin Huang, Lizhen Lin, Gang Wei
Gigantol is a phenolic substance extracted from plants in the genus Dendrobium and used in traditional Chinese medicine. In the present study, we aimed to investigate the growth inhibition and apoptotic effects of gigantol on human liver cancer cells through the PI3K/Akt/NF-κB signaling pathway. HepG2 cells were treated with different concentrations of gigantol (0-150 µM) for 12, 24 and 48 h. It was found that gigantol significantly inhibited the proliferation and induced apoptosis of the HepG2 cells...
February 2017: Oncology Reports
Meirong Ye, Wei Liu, Qingyun Xue, Beiwei Hou, Jing Luo, Xiaoyu Ding
The aim of the current study was to elucidate the phylogeographic history of Dendrobium moniliforme, an endangered orchid species, based on two chloroplast DNA (cpDNA) markers (trnC-petN and trnE-trnT). One hundred and thirty-five samples were collected from 18 natural populations of D. moniliforme covering the entire range of the Sino-Japanese Floristic Region (SJFR) of East Asia. A total of 35 distinct cpDNA haplotypes were identified in these populations, of which 23 haplotypes were each present in only one sample and thus restricted to a single population...
December 8, 2016: Mitochondrial DNA. Part A. DNA Mapping, Sequencing, and Analysis
Xue-Ming Zhou, Cai-Juan Zheng, Jia-Ting Wu, Guang-Ying Chen, Bin Zhang, Chong-Ge Sun
A new phenolic glycoside dendroside (1), together with seven known compounds (2-8) were isolated from the stems of Dendrobium nobile. The structures of these compounds were elucidated using comprehensive spectroscopic methods. The inhibitory activities of all compounds against three cancer cell lines HeLa, MCF-7 and A549 were evaluated.
May 2017: Natural Product Research
M Jia, W Di, Yan Liu, Yin Shi, Y Xie
BACKGROUND: Oxidative stress involved in cryopreservation protocols may be responsible for the poor survival of tissues after cryopreservation. OBJECTIVE: In the current study, we aimed to clarify the role of oxidative stress and its relationship with survival rate during cryopreservation of PLBs from nobile-type Dendrobium. MATERIALS AND METHODS: ROS, antioxidants and oxidative products and the survival rate in PLBs from Dendrobium Hamanal Lake Dream were determined during vitrification...
July 2016: Cryo Letters
Zhi-Gang Wu, Wu Jiang, Song-Lin Chen, Nitin Mantri, Zheng-Ming Tao, Cheng-Xi Jiang
Plant cold acclimation (CA) is a genetically complex phenomenon involving gene regulation and expression. Little is known about the cascading pattern of gene regulatroy network and the link between genes and metabolites during CA. Dendrobium officinale (DOKM) is an important medicinal and ornamental plant and hypersensitive to low temperature. Here, we used the large scale metabolomic and transcriptomic technologies to reveal the response to CA in DOKM seedlings based on the physiological profile analyses. Lowering temperature from 4 to -2°C resulted in significant increase (P < 0...
2016: Frontiers in Plant Science
Abitha Thomas, Ipsita Pujari, Vasudeep Shetty, Manjunath B Joshi, Padmalatha S Rai, Kapaettu Satyamoorthy, Vidhu Sankar Babu
The present study is intended to analyze the occurrence of potent, low produce, naturally occurring stilbenes in protoplasts of wild species and hybrids of Dendrobium. The wild species selected for the study was Dendrobium ovatum, endemic to Western Ghats of India. Protoplasts were isolated from leaves and tepal tissues of all the species and were cultured purely to generate homofusants and cross-cultured to raise heterofusants. Phytochemical composition of protoplast culture with atypical and pure microcolonies was performed using mass spectrometry...
November 11, 2016: Protoplasma
Hongqiang An, Qiankun Zhu, Wei Pei, Jing Fan, Yi Liang, Yihui Cui, Nan Lv, Wanjun Wang
Dendrobium officinale Kimu et Migo has increased many researchers' interest for its high medical and horticultural values and the molecular mechanism of its protocorm development remains unclear. In this study, 19 genes from 26 most stably expressed genes in whole transcriptome of protocorms and 5 housekeeping genes were used as candidate reference genes and screened with 4 application softwares (geNorm, NormFinder, BestKeeper and RefFinder). The results showed that a few reference genes could effectively normalize expression level of specific genes in protocorm development and the optimal top 2 reference genes were ASS and APH1L...
2016: PloS One
Mei Muto, Haruka Asahina, Tatsufumi Yoshioka, Kazuo Ozaki, Makio Shibano
In our investigation, most Shihu (; Japanese name, Sekkoku) in current Japanese commercial crude drugs were from Flickingeria xantholeuca (Orchidaceae). As the index compounds, three new ent-pimarane-type diterpenes, flickinxthanthosides A-C (1-3), one known analogue (7), and three new ent-kaurane-type diterpenes, flickinxanthosides D (4) and E (5) and flickinxanthol A (6) were isolated from the stem of F. xantholeuca. The structures of the new compounds were elucidated on the basis of spectroscopic analyses and chemical methods...
November 3, 2016: Journal of Natural Medicines
Zhihao Zhang, Duoduo Zhang, Mengmeng Dou, Zhubo Li, Jie Zhang, Xiaoyan Zhao
Dendrobium officinale Kimura et Migo (Dendrobium catenatum Lindley), a prized traditional Chinese Medicine, has been used in China and Southeast Asian countries for centuries. The present study was aimed to investigate the effects and the possible mechanisms of the Dendrobium officinale extracts (DOE) on diabetic cardiomyopathy in mice. The diabetic model was induced by intraperitoneal injection of streptozotocin at the dose of 50mg/kg body weight for 5 consecutive days. After 8 weeks treatment of DOE, mice were sacrificed, blood sample and heart tissues were collected...
December 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Feng-Qing Xu, Wei-Wei Fan, Cheng-Ting Zi, Fa-Wu Dong, Dan Yang, Jun Zhou, Jiang-Miao Hu
Four new glucosides, named as gigantol-5-O-β-d-glucopyranoside (1), 9,10-dihydro-aphyllone A-5-O-β-d-glucopyranoside (2), ficusal-4-O-β-d-glucopyranoside (3), botrydiol-15-O-β-d-glucopyranoside (4), together with eight known compounds (5-12) were isolated from the n-BuOH extract of the stems of Dendrobium fimbriatum Hook. Their structures were elucidated by the analyses of spectroscopic data.
April 2017: Natural Product Research
Ruchishree Konhar, Devendra Kumar Biswal, Manish Debnath, Sriram Parameswaran, Durai Sundar, Pramod Tandon
The orchid species Dendrobium nobile belonging to the family Orchidaceae and genus Dendrobium (a vast genus that encompasses nearly 1,200 species) has an herbal medicinal history of about 2000 years in east and south Asian countries. Here, we report the complete chloroplast genome sequence of D. nobile from northeastern India for the first time.
October 6, 2016: Genome Announcements
Meng-Meng Dou, Zhi-Hao Zhang, Zhu-Bo Li, Jie Zhang, Xiao-Yan Zhao
Dendrobium officinale Kimura et Migo has been used for thousands of years to promote body fluid production; however, little is currently known regarding its effects on the heart. The present study aimed to explore the cardioprotective potential of the water extract of Dendrobium officinale Kimura et Migo (DOE) on myocardial ischemia in mice. A mouse model of myocardial ischemia was induced following ligation of the left anterior descending coronary artery. Prior to the operation, mice were administered a vehicle or DOE for 2 weeks...
November 2016: Molecular Medicine Reports
Mu-Jin Lee, Ho-Kyung Jung, Min-Suk Kim, Ji-Hun Jang, Mi-Ok Sim, Tea-Mook Kim, Ho Park, Byung-Kwan Ahn, Hyun-Woo Cho, Jung-Hee Cho, Won-Seok Jung, Jong-Choon Kim
Dendrobium moniliforme (L.) Sw., an herb of the Orchidaceae family, has long been used in traditional medicine to strengthen bones, nourish the stomach, and promote the production of bodily fluid. Recently, polysaccharides isolated from Dendrobium have been used in functional foods and nutraceutical products. A traditional method to process Dendrobium is to soak fresh stems in an ethanol solution, which is the most important factor to ensure high yields of aqueous-extractable polysaccharides. The present study was carried out to investigate the potential acute toxicity of D...
September 2016: Laboratory Animal Research
Xue-Ming Zhou, Cai-Juan Zheng, Jia-Ting Wu, Guang-Ying Chen, Jun Chen, Chong-Ge Sun
Five new lactone derivatives decumbic acids A and B (1 and 2), (-)-decumbic acid (3a), (-)- and (+)-dendrolactone (4a and 4b) together with four known compounds (3b and 5-7) were isolated from the stems of Dendrobium nobile. Their structures were elucidated using comprehensive spectroscopic methods. Compounds 3a and 3b, 4a and 4b were isolated as two pair of enantiomers by chiral HPLC. The absolute configurations of 1, 2, 3a, 4a and 4b were determined by optical rotation and X-ray crystallographic analysis...
December 2016: Fitoterapia
Ji Chen, Feng Deng, Mengsheng Deng, Jincheng Han, Jianbin Chen, Li Wang, Shen Yan, Kai Tong, Fan Liu, Mengliang Tian
Bacterial homologous chloroplast-targeted Obg GTPases (ObgCs) belong to the plant-typical Obg group, which is involved in diverse physiological processes during chloroplast development. However, the evolutionarily conserved function of ObgC in plants remains elusive and requires further investigation. In this study, we identified DoObgC from an epiphytic plant Dendrobium officinale and demonstrated the characteristics of DoObgC. Sequence analysis indicated that DoObgC is highly conserved with other plant ObgCs, which contain the chloroplast transit peptide (cTP), Obg fold, G domain, and OCT regions...
December 2016: DNA and Cell Biology
Xue-Qiang Zha, Yuan-Yuan Deng, Xiao-Long Li, Jing-Fei Wang, Li-Hua Pan, Jian-Ping Luo
The aim of this work was to investigate the core structure of a Dendrobium huoshanense polysaccharide DHPD1 required for the inhibition of lens epithelial cell apoptosis. In order to obtain the fragments containing the core domain, pectinase was employed to hydrolyze DHPD1. After 24h reaction, it is interesting that the hydrolyzation seemed to be stopped, leading to a final enzymatic fragment DHPD1-24 with molecular weight about 1552Da. Compared to DHPD1, although the bioactivity is decreased, DHPD1-24 remained the ability to inhibit the H2O2-induced apoptosis of human lens epithelial (HLE) cells via suppressing the MAPK signaling pathways...
January 2, 2017: Carbohydrate Polymers
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