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Jinyan Cai, Chao Yang, Tianhong Chen, Lin Zhao
Herba Dendrobii has been documented in the Compendium of Materia Medica, with special efficacy in nourishing Yin and treating diabetes. However, the chemical profile of Dendrobium chrysanthum Lindl. has not been reported yet. The compositions were analysed with UPLC/Q-TOF-MS/MS. 53 compounds were tentatively characterised, and 14 compounds might be new compounds. Some novel lactones of poly-phenylpropanoids were firstly detected, and di-p-hydroxyphenylpropionic acidic p-coumaric acid lactone, a new compound of the first example, was obtained and identified...
October 12, 2017: Natural Product Research
Xiao-Man Dong, Chao Jiang, Yuan Yuan, Liang-Ping Zha, Dai-Yin Peng, Yu-Yang Zhao
Based on rDNA ITS sequences of Dendrobium officinale and the other 69 species of Dendrobium, a pair of dismatched allele-specific diagnostic primers, TPSH-AS1F and TPSH-AS1R were designed to authenticate D. officinale from the other species. Thebidirectional PCR amplification were performed using the diagnostic primers with the total DNAs of the original plants or processing products as a template. When the annealing temperature was raised to 60 ℃, only the template DNA of D. officinale could be amplified whereas the diagnostic PCRs of the other Dendrobium species were all negative...
March 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Liu He, Shuhua Fu, Zhichao Xu, Jun Yan, Jiang Xu, Hong Zhou, Jianguo Zhou, Xinlian Chen, Ying Li, Kin Fai Au, Hui Yao
Dendrobium officinale is an extremely valuable orchid used in traditional Chinese medicine, so sought after that it has a higher market value than gold. Although the expression profiles of some genes involved in the polysaccharide synthesis have previously been investigated, little research has been carried out on their alternatively spliced isoforms in D. officinale. In addition, information regarding the translocation of sugars from leaves to stems in D. officinale also remains limited. We analyzed the polysaccharide content of D...
October 5, 2017: Genes
Hui Deng, Nai-Dong Chen, Jun Dai, Nai-Fu Chen
Dendrobium huoshanense is a rare traditional Chinese medicinal herb, and the anti-tumor activity of its polysaccharides is a research hotspot in traditional Chinese medicine resources domain. This study aims to explore the material basis for the anti-tumor activity of polysaccharide. D. huoshanense was used as raw material in the experiment, and the different protein components were obtained through low salt solution extraction and ammonium sulfate fractional precipitation. Then glycoprotein components were determined by SDS-PAGE electrophoresis staining, and were further isolated and purified by DEAE ion column and Sephadex gel column...
January 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Qing Li, Biao Li, Shun-Xing Guo
SSR is one of the most important molecular markers used in molecular identification and genetic diversity research of Dendrobium nobile. In order to enrich the library of SSR and establish a method for rapid identification of D. nobile, the SSR information was analyzed in the transcriptome of D. nobile. A total of 32 709 SSRs were obtained from the transcriptome of D. nobile, distributed in 26 742 unigenes with the distribution frequency of 12.90%. SSR loci occurred every 3 748 bp. Mono-nucleotide repeat was the main type, account for as much as 72...
January 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Yue Chen, Qi Shen, Renan Lin, Zhuangliu Zhao, Chenjia Shen, Chongbo Sun
Artificial control of flowering time is pivotal for the ornamental value of orchids including the genus Dendrobium. Although various flowering pathways have been revealed in model plants, little information is available on the genetic regualtion of flowering in Dendrobium. To identify the critical genes associated with flowering, transcriptomes from four organs (leaf, root, stem and flower) of D. officinale were analyzed in our study. In total, 2645 flower-specific transcripts were identified. Functional annotation and classification suggested that several metabolic pathways, including four sugar-related pathways and two fatty acid-related pathways, were enriched...
September 14, 2017: Plant Physiology and Biochemistry: PPB
Ou Li, Rong Xiao, Lihua Sun, Chenglin Guan, Dedong Kong, Xiufang Hu
As an epiphyte orchid, Dendrobium catenatum relies on microorganisms for requisite nutrients. Metagenome pyrosequencing based on 16S rRNA and nifH genes was used to characterize the bacterial and diazotrophic communities associated with D. catenatum collected from 5 districts in China. Based on Meta-16S rRNA sequencing, 22 bacterial phyla and 699 genera were identified, distributed as 125 genera from 8 phyla and 319 genera from 10 phyla shared by all the planting bases and all the tissues, respectively. The predominant Proteobacteria varied from 71...
2017: PloS One
Song-Zi Xie, Ji-Chun Ge, Fan Li, Jun Yang, Li-Hua Pan, Xue-Qiang Zha, Qiang-Ming Li, Jun Duan, Jian-Ping Luo
With fluorescent end-labeling method, a homogenous Dendrobium huoshanense polysaccharide (GXG) that was orally administrated to mice was found to be degraded into a stable fragment (dGXG) in the stomach, where dGXG was propelled into the small intestine and absorbed into the systemic circulation. The residual GXG in the stomach could pass through the gastrointestinal tract and was excreted into faeces with a very slow fermentation in the large intestine. The simulated digestion of GXG indicated that the acidic pH condition in the gastric fluid was responsible for GXG degradation, which was supported by the oral digestion of dGXG...
September 18, 2017: International Journal of Biological Macromolecules
Hong Zheng, Linlin Pan, Pengtao Xu, Jianjun Zhu, Ruohan Wang, Wenzong Zhu, Yongsheng Hu, Hongchang Gao
Dendrobium officinale Kimura & Migo (D. officinale) is a precious herbal medicine. In this study, we investigated metabolic mechanism underlying the effect of D. officinale water extract (DOWE) on diabetes prevention in mice after streptozotocin (STZ) exposure using NMR-based metabolomics. Interestingly, we found a decrease in blood glucose and an increase in liver glycogen in mice pretreated with DOWE after STZ exposure. The DOWE pretreatment significantly increased citrate and glutamine in the serum as well as creatine, alanine, leucine, isoleucine, valine, glutamine, glutathione and taurine in the liver of STZ-treated mice...
September 15, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Nai-Dong Chen, Tao You, Jun Li, Li-Tao Bai, Jing-Wen Hao, Xiao-Yuan Xu
Plant tissue culture technique is widely used in the conservation and utilization of rare and endangered medicinal plants and it is crucial for tissue culture stocks to obtain the ability to produce similar bioactive components as their wild correspondences. In this paper, a headspace gas chromatography-mass spectrometry method combined with chemometric methods was applied to analyze and evaluate the volatile compounds in tissue-cultured and wild Dendrobium huoshanense Cheng and Tang, Dendrobium officinale Kimura et Migo and Dendrobium moniliforme (Linn...
October 2016: Journal of Food and Drug Analysis
Qingran Meng, Haoran Fan, Feng Chen, Tiancun Xiao, Lianfu Zhang
BACKGROUND: Dendrobium officinale has been used in China for several thousand years as a health food, it has become one of the most expensive tea materials worldwide due to extremely scarce wild resources and increasing demand. Hence, it is very important to improve the depth and width of its application. In present work, the physico-chemical, surface chemistry, and thermal properties of micron range particles and coarse particles prepared by superfine grinding and shear pulverization were investigated...
September 13, 2017: Journal of the Science of Food and Agriculture
Lalita Simpson, Mark A Clements, Darren M Crayn, Katharina Nargar
The Australian mesic biome spans c. 33° of latitude along Australia's east coast and ranges and is dissected by historical and contemporary biogeographical barriers. To investigate the impact of these barriers on evolutionary diversification and to predict the impact of future climate change on the distribution of species and genetic diversity within this biome, we inferred phylogenetic relationships within the Dendrobium speciosum complex (Orchidaceae) across its distribution and undertook environmental niche modelling (ENM) under past, contemporary and projected future climates...
September 6, 2017: Molecular Phylogenetics and Evolution
Yi Zhang, Hongxin Wang, Nana Mei, Chaoyang Ma, Zaixiang Lou, Wenping Lv, GuoHua He
Dendrobium nobile is a medicinal herb in traditional China and Southeast Asian countries. Employing a rat model of ethanol-induced gastric ulcer, we examined the protective effect of polysaccharide (JCP) extracted from Dendrobium nobile and explored the related mechanisms. Oral administration with 100mg/kg and 300mg/kg body weight JCP for days can significant prevent the formation of gastric ulcer. Moreover, JCP pretreatment could alleviate ethanol-induced histological damage, antioxidant activities, the level of epidermal growth factor, gastric concentration of prostaglandin E, and regulate the signaling pathways of mitogen-activated protein kinases and matrix metalloproteinases...
August 31, 2017: International Journal of Biological Macromolecules
Wei Zhang, Qin Wu, Yan-Liu Lu, Qi-Hai Gong, Feng Zhang, Jing-Shan Shi
Dendrobium nobile Lindl. alkaloids (DNLA), the active ingredients of a traditional Chinese medicine Dendrobium, have been shown to have anti-oxidative effects, anti-inflammatory action, and protective effect on neurons against oxygen-glucose deprivation. However, it is not clear whether DNLA reduces amyloid-beta (Aβ)-induced neuronal injury. In this study, cortical neurons were treated with DNLA at different concentrations (0.025, 0.25, and 2.5 mg/L) for 24 hours, followed by administration of Aβ25-35 (10 μM)...
July 2017: Neural Regeneration Research
Cong Zhang, Shou-Jin Liu, Liu Yang, Ming-Yan Yuan, Jin-Yu Li, Bo Hou, Hong-Mei Li, Xing-Zhi Yang, Chang-Chun Ding, Jiang-Miao Hu
A new bibenzyl derivative, dendrocandin V (1) and a new sesquiterpene amino ether, wardianumine A (2), together with eleven known compounds, including phenanthrenes (denbinobin (3), 9,10-dihydro-denbinobin (4), mostatin (5), loddigesiinols A (6)), bibenzyls (moscatilin (7), 5-hydroxy-3,4'-dimethoxybibenzyl (8), 3,4-dihydroxy-5,4'-dimethoxy bibenzyl (9), dendrocandin A (10), gigantol (11), dendrocandin U (12)) and an alkaloids (dihydroshihunine, 13) were isolated from the EtOH extraction of stems of Dendrobium wardianum Warner...
October 2017: Fitoterapia
Chunmei He, Jaime A Teixeira da Silva, Jianwen Tan, Jianxia Zhang, Xiaoping Pan, Mingzhi Li, Jianping Luo, Jun Duan
The WRKY family, one of the largest families of transcription factors, plays important roles in the regulation of various biological processes, including growth, development and stress responses in plants. In the present study, 63 DoWRKY genes were identified from the Dendrobium officinale genome. These were classified into groups I, II, III and a non-group, each with 14, 28, 10 and 11 members, respectively. ABA-responsive, sulfur-responsive and low temperature-responsive elements were identified in the 1-k upstream regulatory region of DoWRKY genes...
August 23, 2017: Scientific Reports
Ji-Ping Si, Qi Wang, Zhong-Jian Liu, Jing-Jing Liu, Yi-Bo Luo
In view of the significant difficulties of propagation, planting and simple product in Dendrobium catenatum(D. officinale)industry development, a series of research were carried out. Genome study showed that D. catenatum is a specie of diploid with 38 chromosomes and 28 910 protein-coding genes. It was identified that specific genes accumulated in different organs at the transcriptome level. We got an insight into the gene regulation mechanism of the loss of the endospermous seed, the wide ecological adaptability and the synthesis of polysaccharides, which provided a theoretical basis for genetic engineering breeding and development and utilization of active pharmaceutical ingredients...
June 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Shangguo Feng, Kaili Jiao, Hong Guo, Mengyi Jiang, Juan Hao, Huizhong Wang, Chenjia Shen
BACKGROUND: Lysine succinylation is a ubiquitous and important protein post-translational modification in various eukaryotic and prokaryotic cells. However, its functions in Dendrobium officinale, an important traditional Chinese orchid herb with high polysaccharide contents, are largely unknown. RESULTS: In our study, LC-MS/MS was used to identify the peptides that were enriched by immune-purification with a high-efficiency succinyl-lysine antibody. In total, 314 lysine succinylation sites in 207 proteins were identified...
August 10, 2017: BMC Genomics
Qiangxiang Li, Jing Chen, Yajia Li, Ting Chen, Jing Zou, Hua Wang
BACKGROUND: The aim of the study was to observe the effect of polysaccharide of dendrobium candidum (PDC) and high glucose on proliferation, apoptosis of human corneal epithelial cells (HCEC). METHODS: The MTT method was used to screen and take the optimal high-glucose concentration, treatment time, and PDC concentration using HCEC and divide it into 4 groups: control group (C), high glucose group (HG), PDC group, and HG + PDC group. We observed and compared the effect of the 4 groups on HCEC proliferation by MTT, apoptosis by Annexin V-FITC/PI double fluorescent staining and flow cytometry (FCM), and expression of bax mRNA and bcl-2 mRNA by RT-qPCR...
August 2017: Medicine (Baltimore)
Zi Ye, Jia-Rong Dai, Cheng-Gang Zhang, Ye Lu, Lei-Lei Wu, Amy G W Gong, Hong Xu, Karl W K Tsim, Zheng-Tao Wang
The stems of Dendrobium officinale Kimura et Migo (Dendrobii Officinalis Caulis) have a high medicinal value as a traditional Chinese medicine (TCM). Because of the limited supply, D. officinale is a high priced TCM, and therefore adulterants are commonly found in the herbal market. The dried stems of a closely related Dendrobium species, Dendrobium devonianum Paxt., are commonly used as the substitute; however, there is no effective method to distinguish the two Dendrobium species. Here, a high performance liquid chromatography (HPLC) method was successfully developed and applied to differentiate D...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
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