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https://www.readbyqxmd.com/read/29243428/-relative-molecular-mass-and-monosaccharide-composition-of-polysaccharide-in-dendrobium-officinale-flowers
#1
Si-Jie Zhang, Zheng Qian, Jing-Jing Liu, Xin-Feng Zhang, Jin-Ping Si
In order to provide new information on polysaccharide in Dendrobium officinale flowers,the monosaccharide composition and relative molecular mass distribution of 11 families of flowers were investigated and analyzed by high performance gel filtration chromatography (HPGFC) and pre-column derivatization ultra performance liquid chromatography (UPLC) in this study. Then cluster analysis was carried out for the monosaccharide peak areas by utilizing SPSS 19.0 software. The results showed that the polysaccharides of all the above 11 hybrid families of D...
October 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29243423/-effects-of-sampling-time-on-polysaccharides-of-dendrobium-catenatum-under-temperature-controlled-condition
#2
Qi Wang, Jing-Jing Liu, Jin-Ping Si, Yu-Qiu Zhu, Lang Qin, Ju-Ling Zhang
In order to reveal the accumulation trend of polysaccharides in Dendrobium catenatum and determine the effect of sampling time on polysaccharides, D. Catenatum D21 clone was harvested from January to December after culturing for 2 to 5 months in the growth chamber with constant temperature. Polysaccharides were determined by phenol-sulfuric acid method and the monosaccharide compositions were analyzed by pre-column derivative-UPLC. The results showed that the content of polysaccharide and its key component mannose was positively correlated with the culture time, but the contents of polysaccharides in all kinds of culture peaked from 5 to 6 months, which were consistent with the trend of field planting...
October 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29234904/post-genomics-era-for-orchid-research
#3
REVIEW
Wen-Chieh Tsai, Anne Dievart, Chia-Chi Hsu, Yu-Yun Hsiao, Shang-Yi Chiou, Hsin Huang, Hong-Hwa Chen
Among 300,000 species in angiosperms, Orchidaceae containing 30,000 species is one of the largest families. Almost every habitats on earth have orchid plants successfully colonized, and it indicates that orchids are among the plants with significant ecological and evolutionary importance. So far, four orchid genomes have been sequenced, including Phalaenopsis equestris, Dendrobium catenatum, Dendrobium officinale, and Apostaceae shengen. Here, we review the current progress and the direction of orchid research in the post genomics era...
December 12, 2017: Botanical Studies (Taipei, Taiwan)
https://www.readbyqxmd.com/read/29217540/cancer-stem-cell-suppressing-activity-of-chrysotoxine-a-bibenzyl-from-dendrobium-pulchellum
#4
Narumol Bhummaphan, Varisa Pongrakhananon, Boonchoo Sritularak, Pithi Chanvorachote
Cancer stem cells (CSCs) have been recognized as rare populations driving cancer progression, metastasis, and drug resistance in leading cancers. Attempts have been paid toward identifying compound that specifically targets these CSCs. Therefore, the investigations of the novel therapeutic strategies for CSCs targeting is required. The cytotoxic effects of Chrysotoxine on human NSCLC-derived H460 and H23 cells were tested by MTT assay. The Effect of Chrysotoxine suppresses CSC-like phenotypes was determined in CSC-rich populations and primary CSCs in 3D culture and Extreme limiting dilution assay...
December 7, 2017: Journal of Pharmacology and Experimental Therapeutics
https://www.readbyqxmd.com/read/29203109/influence-of-light-intensity-and-water-content-of-medium-on-total-dendrobine-of-dendrobium-nobile-lindl
#5
Jin-Ling Li, Zhi Zhao, Hong-Chang Liu, Chun-Li Luo, Hua-Lei Wang
OBJECTIVE: To ascertain the influence of light intensity and water content of medium on the total dendrobine of Dendrobium nobile (D. nobile). METHOD: The principal component analysis combined with total dendrobine accumulation was conducted to assess the yield and quality of D. nobile in all treatments. In the experiment, D. nobile plants were cultivated in greenhouse as tested materials, and complete test of 9 treatments was adopted with relative light intensities 75...
November 2017: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/29186512/doft-and-doftip1-affect-reproductive-development-in-the-orchid-dendrobium-chao-praya-smile
#6
Yanwen Wang, Lu Liu, Shiyong Song, Yan Li, Lisha Shen, Hao Yu
FLOWERING LOCUS T (FT) in Arabidopsis encodes the florigen that moves from leaves to the shoot apical meristem to induce flowering, and this is partly mediated by FT-INTERACTING PROTEIN 1 (FTIP1). Although FT orthologs have been identified in some flowering plants, their endogenous roles in Orchidaceae, which is one of the largest families of flowering plants, are still largely unknown. In this study, we show that DOFT and DOFTIP1, the orchid orthologs of FT and FTIP1, respectively, play important roles in promoting flowering in the orchid Dendrobium Chao Praya Smile...
November 24, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/29181391/barcoding-the-dendrobium-orchidaceae-species-and-analysis-of-the-intragenomic-variation-based-on-the-internal-transcribed-spacer-2
#7
Xiaoyue Wang, Xiaochen Chen, Pei Yang, Lili Wang, Jianping Han
Many species belonging to the genus Dendrobium are of great commercial value. However, their difficult growth conditions and high demand have caused many of these species to become endangered. Indeed, counterfeit Dendrobium products are common, especially in medicinal markets. This study aims to assess the suitability of the internal transcribed spacer 2 (ITS2) region as a marker for identifying Dendrobium and to evaluate its intragenomic variation in Dendrobium species. In total, 29,624 ITS2 copies from 18 species were obtained using 454 pyrosequencing to evaluate intragenomic variation...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29171225/-effects-of-cultivation-environments-on-dendrobium-catenatum
#8
Yi-Kai Lin, Yu-Qiu Zhu, Jin-Ping Si, Lang Qin, Yan Zhu, Ling-Shang Wu, Jing-Jing Liu
The study was aimed to clarify the effect of three cultivation environments on the growth and metabolism of Dendrobium catenatum C13 group. There were three different cultivation conditions including rock epiphytic cultivation, pear epiphytic cultivation and pot cultivation. Morphological characteristics and agronomic characters of D. catenatum were observed and measured. Microstructure, contents of polysaccharide and alcohol-soluble extracts were measured by paraffin section method, phenol-sulfuric acid method and hot-dip method, respectively...
August 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29168730/comparative-proteomics-analyses-of-pollination-response-in-endangered-orchid-species-dendrobium-chrysanthum
#9
Wei Wang, Hongyang Yu, Tinghai Li, Lexing Li, Guoqiang Zhang, Zhongjian Liu, Tengbo Huang, Yongxia Zhang
Pollination is a crucial stage in plant reproductive process. The self-compatibility (SC) and self-incompatibility (SI) mechanisms determined the plant genetic diversity and species survival. D. chrysanthum is a highly valued ornamental and traditional herbal orchid in Asia but has been declared endangered. The sexual reproduction in D. chrysanthum relies on the compatibility of pollination. To provide a better understanding of the mechanism of pollination, the differentially expressed proteins (DEP) between the self-pollination (SP) and cross-pollination (CP) pistil of D...
November 23, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29150333/design-synthesis-and-evaluation-of-novel-dihydrostilbene-derivatives-as-potential-anti-melanogenic-skin-protecting-agents
#10
Yu Zhu, Wen-Hui Pan, Chuen Fai Ku, Hong-Jie Zhang, Siu Wai Tsang
The stems of Dendrobium orchids (Orchidaceae), also known as Shi Hu, have been used for medicinal purposes for centuries in oriental countries. In fact, the health benefits of Shi Hu have been evidenced by its modern pharmacological actions on conquering oxidative stress in pathological conditions. From the extracts of two commonly used Dendrobium species, we obtained discernible amounts of stilbenoids, explicitly trans-resveratrol (1) and dihydro-resveratrol (2), which are prototypical antioxidants. When applied to cultured melanocytes, these stilbenoids, dihydro-resveratrol (2) in particular, significantly reduced melanin formation via inhibiting tyrosinase activity and expression of tyrosinase-related proteins...
October 10, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29103502/chemical-characterization-and-immunomodulatory-activity-of-acetylated-polysaccharides-from-dendrobium-devonianum
#11
Yong Deng, Min Li, Ling-Xiao Chen, Xian-Qiang Chen, Jia-Hong Lu, Jing Zhao, Shao-Ping Li
The chain conformation, chemical characters and immunomodulatory activity of polysaccharide from Dendrobium devonianum (DDP) were investigated. Results showed that molecular weights, polydispersity index, radius of gyrations of DDP were 3.99×10(5) Da1.27, 74.1nm, respectively. By applying the polymer solution theory, the exponent (v) values of <S(2)>z(1/2)=kMw(v) was calculated as 0.38, which revealed that DDP existed as a globular shape in aqueous solution, and further confirmed by AFM analysis. Furthermore, the main monosaccharide compositions were Man and Glc with the ratio of 29...
January 15, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29099062/mutational-biases-and-gc-biased-gene-conversion-affect-gc-content-in-the-plastomes-of-dendrobium-genus
#12
Zhitao Niu, Qingyun Xue, Hui Wang, Xuezhu Xie, Shuying Zhu, Wei Liu, Xiaoyu Ding
The variation of GC content is a key genome feature because it is associated with fundamental elements of genome organization. However, the reason for this variation is still an open question. Different kinds of hypotheses have been proposed to explain the variation of GC content during genome evolution. However, these hypotheses have not been explicitly investigated in whole plastome sequences. Dendrobium is one of the largest genera in the orchid species. Evolutionary studies of the plastomic organization and base composition are limited in this genus...
November 2, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29092642/a-new-propylphenyl-bibenzyl-derivative-from-dendrobium-williamsonii
#13
Miao Yang, Yan Zhang, Lijun Chen, Yegao Chen
A new (propylphenyl)bibenzyl derivative, dendrowillol A (1), along with 22 known compounds were isolated from the whole plants of Dendrobium williamsonii. Their structures were elucidated by means of extensive spectroscopic analysis. Cytotoxicity evaluation against five human tumour lines showed that all the compounds tested displayed very weak cytotoxicity when compared with the positive control taxol, but selective inhibitory effects on HL-60 for several compounds tested were observed, with IC50 values ranging from 4...
November 2, 2017: Natural Product Research
https://www.readbyqxmd.com/read/29090564/-herbal-textual-research-on-relationship-between-chinese-medicine-shihu-dendrobium-spp-and-tiepi-shihu-d-catenatum
#14
Jin-Ping Si, Yuan Zhang, Yi-Bo Luo, Jing-Jing Liu, Zhong-Jian Liu
Dendrobium species on the ancient Chinese herbal texts were investigated in this paper, including their dscriptions of original species, producing areas and quality. Our results indicated that the major producing areas were Lu'an, Anhui province and Wenzhou, Taizhou, Zhejiang province. In addition, the sweet flavor, short, thin and solid stems were standing for good quality. Based on the stable producing areas and quality descriptions, D. catenatum (D. officinale) ("Tiepi Shihu") and D. houshanense were high quality medicinal Dendrobium species ("Shihu" ) in ancient China...
May 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29090555/-protective-effects-of-five-different-types-of-dendrobium-on-ccl4-induced-liver-injury-in-mice
#15
Kai Wang, Dan-Juan Sui, Chang-Suo Wang, Li Yang, Zhen Ouyang, Nai-Fu Chen, Bang-Xing Han, Yuan Wei
This study aims to investigate the protective effect of Dendrobium huoshanense, D.officinale(Huoshan), D.officinale(Yunnan), D.moniliforme and D. henanense on CCl4-induced hepatic damage in mice. C57BL/6 mice were randomly divided into control group, model group, high-dose(7.5 g•kg⁻¹) and low-dose (1.25 g•kg⁻¹) groups of the five Dendrobium. Each group was intragastrically administered with drugs for 2 weeks. The control group was intraperitoneally injected with Olive oil solution, while the other groups were intraperitoneally given 0...
May 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29074081/protective-roles-and-mechanisms-of-dendrobium-officinal-polysaccharides-on-secondary-liver-injury-in-acute-colitis
#16
Jian Liang, Shuxian Chen, Youdong Hu, Yiqi Yang, Jun Yuan, Yanfang Wu, Shijie Li, Jizhong Lin, Lian He, Shaozhen Hou, Lian Zhou, Song Huang
The purpose of this study was to investigate the protective roles and mechanisms of Dendrobium officinale polysaccharides (DOPS) on secondary liver injury in acute colitis. Firstly, the mice model of secondary liver injury in acute colitis was induced by 4% Dextran sodium sulfate (DSS). Inflammatory cell model was established by LPS-stimulated RAW264.7 cells. Then, the protective roles of DOPS were evaluated by both in vivo and in vitro experiment. The results showed that DOPS attenuated DSS-induced hepatic pathological damage, liver parameters, infiltration of macrophages, cytokines levels, MDA level and increased the antioxidant enzymes activities...
October 23, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29069925/two-new-picrotoxane-type-sesquiterpenoid-lactones-from-dendrobium-williamsonii
#17
Miao Yang, Li-Jun Chen, Yan Zhang, Ye-Gao Chen
Two new picrotoxane-type sesquiterpenoid lactones, dendrowillins A (1) and B (2), together with five known sesquiterpenoids, (-)-picrotin (3), α-dihydropicrotoxinin (4), dendronobilin B (5), amoenin (6), and (-)-10β,13,14-trihydroxyalloaromadendrane (7), were isolated from the whole plants of Dendrobium williamsonii. Their structures were elucidated by means of extensive spectroscopic analysis.
October 25, 2017: Journal of Asian Natural Products Research
https://www.readbyqxmd.com/read/29063360/new-phenanthrene-and-9-10-dihydrophenanthrene-derivatives-from-the-stems-of-dendrobium-officinale-with-their-cytotoxic-activities
#18
Gui-Yun Zhao, Bo-Wen Deng, Chong-Yu Zhang, Yi-Da Cui, Jia-Yi Bi, Guo-Gang Zhang
Two new phenanthrene and 9, 10-dihydrophenanthrene derivatives (1-2) with six known congeners (3-8) were isolated from the extraction of stems of Dendrobium officinale. Compounds 1 and 2 were based on carbon skeleton in which phenanthrene and 9, 10-dihydrophenanthrene moiety were linked with a phenylpropane unit through a dioxane bridge, respectively. Their structures were determined by comprehensive NMR spectroscopic data, the absolute configuration of new compounds were determined by comparing their experimental and calculated ECD for the first time...
October 23, 2017: Journal of Natural Medicines
https://www.readbyqxmd.com/read/29061811/batatasin-iii-inhibits-migration-of-human-lung-cancer-cells-by-suppressing-epithelial-to-mesenchymal-transition-and-fak-akt-signals
#19
Tatchakorn Pinkhien, Nareerat Petpiroon, Boonchoo Sritularak, Pithi Chanvorachote
BACKGROUND/AIM: Lung cancer is the leading cause of cancer-related deaths worldwide. Compound Batatasin III isolated from Dendrobium draconis Rchb.f. was tested for the possible anti-cancer activities including anti-proliferative, anti-migration and invasion in human non-small lung cancer H460 cells. MATERIALS AND METHODS: The effect of Batatasin III on viability and proliferation of H460 cells was investigated by the 3-[4,5-dimethylthiazol-2-yl]-2,5diphenyl tetrazoliumbromide (MTT) assay...
November 2017: Anticancer Research
https://www.readbyqxmd.com/read/29022365/detection-of-new-phenylpropanoids-from-dendrobium-chrysanthum
#20
Jinyan Cai, Chao Yang, Tianhong Chen, Lin Zhao
Herba Dendrobii has been documented in the Compendium of Materia Medica, with special efficacy in nourishing Yin and treating diabetes. However, the chemical profile of Dendrobium chrysanthum Lindl. has not been reported yet. The compositions were analysed with UPLC/Q-TOF-MS/MS. 53 compounds were tentatively characterised, and 14 compounds might be new compounds. Some novel lactones of poly-phenylpropanoids were firstly detected, and di-p-hydroxyphenylpropionic acidic p-coumaric acid lactone, a new compound of the first example, was obtained and identified...
October 12, 2017: Natural Product Research
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