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Yi-Min Li, Ying Chen, A-Ping Liu, Na Zhang, Xiao-Bin Hei, Gang Zhang, Shun-Xing Guo
In this study, RACE technology was employed to isolate the full length cDNA of DoHT1 in Dendrobium officinale, followed by bioinformatics analysis of the sequence characteristics. And the expression pattern of the gene was also analyzed by quantitative PCR. The full length cDNA of DoHT1 was 1 586 bp in length, containing a 1 536 bp ORF, which encoded a 511-aa protein with molecular weight of 56.18 kD and isoelectric point of 9.08. The deduced DoHT1 protein had the major facilitator superfamily conserved domain (22-483), SUGAR₋TRANSPORT₋1 (139-164), and SUGAR₋TRANSPORT₋2 (338-355), typical for sugar transporter; DoHT1, without a single peptide had 11 transmembrane regions, and was predicted to locate in the plasma membrane; DoHT1 had high identities (54...
March 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Jian Yang, Hao Wu, Chao-Geng Lv, Sheng Wang, Xiao-Bo Zhang, Guang Yang, Lan-Ping Guo, Lu-Qi Huang
The isotopic ratios of strontium isotope (Sr) and light elements (C/H/O/N) in Dendrobium officinale from different producing areas (Shaoguan, Guangdong; Yulin, Guangxi; Shibing, Guizhou; Wenshan, Yunnan and Zhejiang province) were determined with thermal ionization mass spectrometry (TI-MS) and isotope ratio mass spectrometry (IRMS).The differences of the stable isotope ratios in D. officinale were obtained by the variance analysis and the correlation analysis, and pattern recognition techniques with principal component analysis (PCA) was used to classify the geographical origins of D...
March 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Chunhua Zhou, Zhenshan Xie, Zhouxi Lei, Yuechun Huang, Gang Wei
BACKGROUND: The quality of material medicine resources has had a considerable impact on the development of the health industry, which has created a bottleneck for traditional Chinese medicine (TCM). Dendrobium officinale, which has been widely used for health prevention in TCM, has become a high-nutritive health food that is strongly recommended by many white-collar workers and people who pay more attention to their health. The aim of this study was to develop a method to authenticate and evaluate D...
April 12, 2018: Chemistry Central Journal
Tomoko Takamiya, Saki Kitamura, Shunichiro Suzuki, Nao Shioda, Yoshifumi Matsuo, Kento Murase, Yuka Kaiho, Yurika Tsurumaki, Yukiko Fujiwara, Mayumi Sone, Tomomi Machida, Ryohei Matsumoto, Aoi Miyamoto, Dai Hirose, Megumi Furukawa, Mitsuko Makino, Keiichi Matsuzaki, Susumu Kitanaka, Tomohisa Yukawa, Hiroshi Iijima
Genus Dendrobium (Orchidaceae) contains numerous species. Phylogenetic analyses based on morphological characteristics and DNA sequences indicated that this genus is divided into two major groups: Asian and Australasian clades. On the other hand, little is known about the phytochemical differences and similarities among the species in each clade. In this study, we selected 18 Dendrobium species (11 from the Asian clade and 7 from the Australasian clade) and constructed HPLC profiles, arrays composed of relative intensity of the chromatographic peaks...
April 5, 2018: Chemical & Pharmaceutical Bulletin
Siyan Zheng, Yingpeng Zhu, Chunyan Jiao, Mengyao Shi, Lianping Wei, Yang Zhou, Qing Jin, Yongping Cai
In order to optimize the extraction of gigantol from Dendrobium officinale , the influence of methanol concentration, ultrasonic temperature, and liquid ratio on extraction efficiency was analysed by the response surface analysis method. The results show that the extraction rate reached a maximum when the methanol concentration was 92.98%, the solid-liquid ratio was 27.2 mL/g, and the extraction temperature was 41.41 °C. The content of gigantol of Dendrobium officinale in leaves was significantly higher than that in stems, reaching 4...
April 3, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Xiao-Chun Xiao, Wei-Hong Chen, Yuan-Yuan Cao, Yong-Jun Lou, Yuan Liu, Jin Wang, Xu-Feng Zhai, Nian-Sheng Li, Ying Li
To study the effect and mechanism of Dendrobium candidum on isoproterenol-induced myocardial hypertrophy in rats, 60 healthy SD rats(30 males and 30 females) were randomly divided into 5 groups(12 in each group): normal group, model group, three D. candidum preventive administration groups(0.09, 0.18, 1.1 g·kg⁻¹). Except for the normal group, rats of other groups were injected back subcutaneously with ISO(5 mg·kg⁻¹) for 10 consecutive days. At the same time, preventive administration groups began to give different doses of the sample for 30 days and model group began to give normal saline...
February 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Donghui Wang, Bei Fan, Yan Wang, Lijing Zhang, Fengzhong Wang
Response surface methodology (RSM) was employed to optimize the conditions for the ultrasonic-assisted extraction (UAE) of polysaccharides from the flowers of Dendrobium devonianum . The optimal conditions for the maximum yields of DDFPs are as follows: an extraction temperature of 63.13°C, an extraction time of 53.10 min, and a water-to-raw material ratio of 22.11 mL/g. Furthermore, three fractions (DDFPs30, DDFPs50, and DDFPs70) were prepared from Dendrobium devonianum flowers polysaccharides (DDFPs) by the stepwise ethanol precipitation method...
2018: International Journal of Analytical Chemistry
Ji-Chun Ge, Xue-Qiang Zha, Chun-Yan Nie, Nian-Jun Yu, Qiang-Ming Li, Dai-Yin Peng, Jun Duan, Li-Hua Pan, Jian-Ping Luo
The present work investigated the inhibitory activity of the polysaccharide from cultivated Dendrobium huoshanense (cDHP) on lung inflammation in cigarette smoke (CS)-induced mouse model. cDHP was mainly composed of mannose and glucose in a molar ratio of 1.89: 1.00, and had a backbone with linkages of 1,4-Manp, 1,4-Glcp, 1,4,6-Manp and 1-Glcp. Hematoxylin and Eosin (HE) staining and immunohistochemistry analysis showed that cDHP can increase alveolar number, thicken alveolar wall, inhibit pulmonary bulla formation and decrease inflammatory cell infiltration as compared to the model group...
June 1, 2018: Carbohydrate Polymers
Chao Yang, Weilong Lin, Lin Zhao, Jinyan Cai
A new furostanol saponin, (25R)-26-O-(α-d-glucopyranosyl)-(1→2)-α-l-rhamnopyranosyl-furost-5-ene-3β, 22α, 26-triol-3-O-α-d-glucopyranoside (1), together with four known compounds 2-5 were isolated from the ethanolic extract of the stems of Dendrobium chrysanthum Lindl. The structures of these new compounds were identified by extensive spectroscopic analysis including 1D and 2D NMR and HR-ESI-MS, as well as chemical methods. Compounds 1-3 were isolated from D. chrysanthum for the first time. Furthermore, the inhibitory effects of the compounds on tumor cells were evaluated, and compounds 1-2 exhibited significant cytotoxic activities potentially against SPC-A1, MCF-7 and HeLa human cancer cell lines...
March 26, 2018: Natural Product Research
Salinee Na Ranong, Kittisak Likhitwitayawuid, Wanwimon Mekboonsonglarp, Boonchoo Sritularak
Two new dihydrophenanthrenes, dendroinfundin A (1) and dendroinfundin B (2) were isolated from the whole plant of Dendrobium infundibulum, together with 7 known compounds (3-9). The structures of the new compounds (1 and 2) were established on the basis of their spectroscopic data. All of the isolates were evaluated for their α-glucosidase and pancreatic lipase inhibitory activities. Batatasin III (5) and dendrosinen B (9) showed strong α-glucosidase inhibitory activity. Dendrosinen B (9) also exhibited appreciable pancreatic lipase inhibitory effect...
March 23, 2018: Natural Product Research
Huiying Ma, Keke Zhang, Qing Jiang, Diya Dai, Hongli Li, Wentao Bi, David Da Yong Chen
Plant polysaccharides have numerous medicinal functions. Due to the differences in their origins, regions of production, and cultivation conditions, the quality and the functions of polysaccharides can vary significantly. They are macromolecules with large molecular weight (MW) and complex structure, and pose great challenge for the analytical technology used. Taking Dendrobium officinale (DO) from various origins and locations as model samples. In this investigation, mechanochemical extraction method was used to successfully extract polysaccharides from DO using water as solvent, the process is simple, fast (40 s) and with high yield...
March 13, 2018: Journal of Chromatography. A
Kai-Lun Liang, Ping Fang, Qiu-Qiu Shi, Jie Su, Bo Li, Su-Hong Chen, Gui-Yuan Lv
This study aimed to investigate the antihypertensive effect and possible mechanism of Dendrobium officinale flos on hypertensive rats induced by high glucose and high fat compound alcohol. The hypertensive models were successfully made by high-glucose and high-fat diet, with gradient drinking for 4 weeks, and then divided into model control group, valsartan (5.7 mg·kg⁻¹) positive control group and D. officinale flos groups (3,1 g·kg⁻¹). After 6 weeks of treatment, the blood pressure of rats was measured regularly...
January 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Ming-Yue Wang, Chang Shen, Meng-Fei An, Chuan-Qi Xie, Xin Wu, Qiang-Qiang Zhu, Bin Sun, Yan-Ping Huang, Yun-Li Zhao, Xuan-Jun Wang, Jun Sheng
AIMS: Dendrobium candidum (DC) and black tea, are traditional chinese drinks, which contain multiple active ingredients. However, whether or not the combination of these two ingredients can improve osteoporosis remains unknown. This study therefore aimed to examine the effects of the combination of DC and black tea extract (BTE) on osteoporosis. MAIN METHODS: Ovariectomy (OVX)-induced osteoporosis in vivo as well as receptor activator of NF-κB ligand (RANKL)-induced osteoclastogenesis in vitro was selected...
March 12, 2018: Life Sciences
Li-Chan Yang, Jiunn-Wang Liao, Chi-Luan Wen, Wen-Chuan Lin
Dendrobium Taiseed Tosnobile is a new species of herba dendrobii (Shi-Hu) that was developed by crossbreeding D. tosaense and D. nobile . Its pharmacological activity and active component have been reported, but its subchronic toxicity and genetic safety have not yet been investigated. This study assessed the 90-day oral toxicity and genetic safety of the aqueous extracts of D. Taiseed Tosnobile (DTTE) in male and female Sprague-Dawley (SD) rats. Eighty rats were divided into four groups, each consisting of ten male and ten female rats...
2018: Evidence-based Complementary and Alternative Medicine: ECAM
Onsurang Wattanathamsan, Surassawadee Treesuwan, Boonchoo Sritularak, Varisa Pongrakhananon
In original publication of the article, unfortunately one of the author names was published incorrectly as "Boonchu Sritularuk". The correct name is "Boonchoo Sritularak".
March 13, 2018: Journal of Natural Medicines
Chunyan Jiao, Cheng Song, Siyan Zheng, Yingpeng Zhu, Qing Jin, Yongping Cai, Yi Lin
Alkaloids are the main active ingredients in the medicinal plant Dendrobium officinale . Based on the published genomic and transcriptomic data, a proposed terpenoid indole alkaloid (TIA) biosynthesis pathway may be present in D. officinale . In this study, protocorm-like bodies (PLBs) with a high-yielding production of alkaloids were obtained by the optimization of tryptophan, secologanin and methyl jasmonate (MeJA) treatment. The results showed that the total alkaloid content was 2.05 times greater than that of the control group when the PLBs were fed with 9 µM tryptophan, 6 µM secologanin and 100 µM MeJA after 36 days...
March 3, 2018: International Journal of Molecular Sciences
Anirut Hlosrichok, Somruethai Sumkhemthong, Boonchoo Sritularak, Pithi Chanvorachote, Chatchai Chaotham
Failure of current chemotherapeutic drugs leads to the recurrence of tumor pathology and mortality in lung cancer patients. This study aimed to evaluate the anticancer activity and related mechanisms of 4,5,4'-trihydroxy-3,3'-dimethoxybibenzyl (TDB), a bibenzyl extracted from Dendrobium ellipsophyllum Tang and Wang, in human lung cancer cells. Cytotoxicity of TDB (0-300 µM) in different types of human lung cancer cells (H460, H292 and H23) and human dermal papilla cells (DPCs) was evaluated via MTT viability assay...
February 27, 2018: Journal of Natural Medicines
Yang Hu, Jie Ren, Lei Wang, Xin Zhao, Mian Zhang, Kuniyoshi Shimizu, Chaofeng Zhang
Dendrobium crepidatum was one of the sources of Herba Dendrobii, a famous and precious traditional Chinese medicine. Indolizine-type alkaloids are the main characteristic ingredients of D. crepidatum, which possesses a variety of changeable skeletons. In the present study, we found that the total alkaloids of D. crepidatum (TAD) can inhibit the production of nitric oxide (NO) in lipopolysaccharide (LPS)-activated macrophages and showed protective effects against LPS-induced acute lung injury (ALI) in mice through downregulating the TLR4-mediated MyD88/MAPK signaling pathway...
February 10, 2018: Phytochemistry
Onsurang Wattanathamsan, Surassawadee Treesuwan, Boonchu Sritularuk, Varisa Pongrakhananon
The life-threatening potential of lung cancer has increased over the years due to its acquisition of chemotherapeutic resistance, especially to cisplatin, a first-line therapy. In response to this development, researchers have turned their attention to several compounds derived from natural origins, including cypripedin (CYP), a phenanthrenequinone substance extracted from Dendrobium densiflorum. The aim of the present study was to investigate the ability of CYP to induce apoptosis and enhance cisplatin-mediated death of human lung cancer NCI-H460 cells using cell viability and apoptosis assays...
February 9, 2018: Journal of Natural Medicines
Jian Liang, Shuxian Chen, Jianhui Chen, Jizong Lin, Qingping Xiong, Yiqi Yang, Jun Yuan, Lian Zhou, Lian He, Shaozhen Hou, Shijie Li, Song Huang, Xiaoping Lai
Polysaccharide, as a promising candidate to meet the medication requirement of ulcerative colitis (UC), is increasingly attracting extensive interest. Dendrobium officinale has been widely used to treat gastrointestinal sickness in the clinical treatment of Traditional Chinese Medicine. However, it remains largely unknown whether polysaccharides (DOPS) from Dendrobium officinale can treat UC. The purpose of this paper is to confirm therapeutic action of DOPS to UC and explored its underlying mechanisms. We noted that DOPS could dramatically improve clinical signs and symptoms, decrease mortality, alleviate colonic pathological damage, and reestablish the balance of pro- and anti-inflammatory cytokines in DSS-induced acute UC mice...
April 1, 2018: Carbohydrate Polymers
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