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https://www.readbyqxmd.com/read/28385810/regulation-of-p53-mediated-changes-in-the-upa-fibrinolytic-system-and-in-lung-injury-by-loss-of-surfactant-protein-c-expression-in-alveolar-epithelial-cells
#1
Bijesh Puthusseri, Amarnath S Marudamuthu, Nivedita Tiwari, Jian Fu, Steven Idell, Sreerama Shetty
Pulmonary surfactant protein-C (SP-C) expression by type II alveolar epithelial cells (AECs) is markedly reduced in diverse types of lung injuries and is often associated with AEC apoptosis. It is unclear whether loss of SP-C contributes to the increased p53 and urokinase-type plasminogen activator (uPA) system cross talk and apoptosis of AECs. We therefore inhibited SP-C expression in human and murine AECs using lentivirus vector expressing shRNA and tested p53 and downstream changes in uPA-fibrinolytic system...
April 6, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28378510/nonparametric-bayesian-covariate-adjusted-estimation-of-the-youden-index
#2
Vanda Inácio de Carvalho, Miguel de Carvalho, Adam J Branscum
A novel nonparametric regression model is developed for evaluating the covariate-specific accuracy of a continuous biological marker. Accurately screening diseased from nondiseased individuals and correctly diagnosing disease stage are critically important to health care on several fronts, including guiding recommendations about combinations of treatments and their intensities. The accuracy of a continuous medical test or biomarker varies by the cutoff threshold (c) used to infer disease status. Accuracy can be measured by the probability of testing positive for diseased individuals (the true positive probability or sensitivity, Se(c), of the test), and the true negative probability (specificity, Sp(c)) of the test...
April 4, 2017: Biometrics
https://www.readbyqxmd.com/read/28352858/n-heterocyclic-carbene-copper-catalyzed-direct-alkylation-of-terminal-alkynes-with-non-activated-alkyl-triflates
#3
Liqun Jin, Wangfang Hao, Jianeng Xu, Nan Sun, Baoxiang Hu, Zhenlu Shen, Weimin Mo, Xinquan Hu
(NHC)-Cu-catalyzed C(sp)-C(sp(3)) bond formation has been successfully achieved under mild conditions. Nonactivated alkyl triflates, which could be easily derived from alcohols, were utilized as C-O electrophiles. Mechanistic studies suggested that copper acetylide was the active species. Scale-up reactions further demonstrated the practicality and efficiency of the developed strategy.
March 29, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28287822/efficiency-and-specificity-of-gene-deletion-in-lung-epithelial-doxycycline-inducible-cre-mice
#4
Meenal Sinha, Clifford A Lowell
Transgenic mouse strains, SP-C-rtTA, CCSP-rtTA and TetO-Cre, have been invaluable in spatiotemporally regulating gene deletion in pulmonary epithelium. In this study, we measured the efficiency and specificity of gene deletion achievable in these mice using the Rosa26-eYFP reporter. Triple transgenic mice (tTg or rtTA/TetO-Cre/Rosa-eYFP) were bred and treated with various doxycycline (dox) regimes to induce gene deletion, which was then quantified in various cell populations by flow cytometry. In these crosses, we found that the TetO-Cre transgene must be transmitted through the female parent to avoid germline gene deletion...
March 13, 2017: American Journal of Respiratory Cell and Molecular Biology
https://www.readbyqxmd.com/read/28282487/establishment-of-multiplex-solid-phase-strip-pcr-test-for-detection-of-24-ocular-infectious-disease-pathogens
#5
Satoko Nakano, Sunao Sugita, Yasuhiro Tomaru, Ayumi Hono, Takako Nakamuro, Toshiaki Kubota, Hiroshi Takase, Manabu Mochizuki, Masayo Takahashi, Norio Shimizu
Purpose: To establish and evaluate a new multiplex solid-phase strip polymerase chain reaction (strip PCR) for concurrent detection of common ocular infectious disease pathogens. Methods: A new multiplex strip PCR was established to detect 24 common ocular infectious disease pathogens: herpes simplex virus (HSV) 1, HSV2, varicella-zoster virus (VZV), Epstein-Barr virus (EBV), cytomegalovirus (CMV), human herpes virus (HHV) 6, HHV7, HHV8, human T-cell lymphotropic virus (HTLV)-1, adenovirus, Mycobacterium tuberculosis, Treponema pallidum, Propionibacterium acnes (P...
March 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28247170/synthesis-and-biological-evaluation-of-arylpyrazoles-as-fungicides-against-phytopathogenic-fungi
#6
Jin Zhang, Ju-Fang Peng, Tao Wang, Yang Kang, Sisi Jing, Zun-Ting Zhang
3-phenol-1H-pyrazoles (2), 4-halogeno-3-phenol-1H-pyrazoles (3) and 2-(1-phenol-1H-pyrazol-5-yl)phenols (4) were prepared by the condensation of (E)-3-(dimethylamino)-1-phenylprop-2-en-1-ones and hydrazine hydrate or phenylhydrazine in good yields. They were evaluated against five phytopathogens fungi, namely Cytospora sp., Colletotrichum gloeosporioides, Botrytis cinerea, Alternaria solani and Fusarium solani in vitro. Most of the above-mentioned compounds exhibited activities. For example, 4-chloro-2-(1H-pyrazol-3-yl)phenol (3k) and 4-bromo-3-phenol-1H-pyrazole (3b) showed good and broad-spectrum antifungal properties against Cytospora sp...
February 28, 2017: Molecular Diversity
https://www.readbyqxmd.com/read/28229215/sequence-analysis-and-expression-of-a-blue-light-photoreceptor-gene-slwc-1-from-the-cauliflower-mushroom-sparassis-latifolia
#7
Chi Yang, Lu Ma, Zhenghe Ying, Xiaoling Jiang, Yanquan Lin
Light is a necessary environmental factor for fruit body formation and development of the cauliflower mushroom Sparassis latifolia, a well-known edible and medicinal fungus. In this study, we firstly characterized the SP-C strain, which belonged to S. latifolia. And then we cloned and sequenced a photoreceptor gene (Slwc-1) from S. latifolia. The product of Slwc-1, SlWC-1 (872 aa residues) contained a coiled-coil region, a LOV domain, and two PAS domains. Phylogenetic tree result showed that SLWC-1 was most close to GfWC-1 from Grifola frondosa in edible and medicinal fungus...
April 2017: Current Microbiology
https://www.readbyqxmd.com/read/28221717/cerebral-and-lung-effects-of-a-new-generation-synthetic-surfactant-with-sp-b-and-sp-c-analogs-in-preterm-lambs
#8
Carmen Rey-Santano, Victoria E Mielgo, Xabier Murgia, Miguel Angel Gomez-Solaetxe, Fabrizio Salomone, Federico Bianco, Nicola Pelizzi, Begoña Loureiro, Jon López de Heredia Y Goya
BACKGROUND: Though natural surfactants (SF) are clinically superior to protein-free synthetic preparations, CHF-5633, a synthetic SF containing SP-B and SP-C analog peptides is a potential alternative to natural SF for treating neonatal respiratory distress syndrome (RDS). Nevertheless, information is lacking regarding the safety of this new treatment for the neonatal brain. We sought to compare the cerebral and pulmonary effects of this new synthetic surfactant (CHF5633) with those of natural porcine surfactant (Cursosurf) in premature lambs with RDS...
February 21, 2017: Pediatric Pulmonology
https://www.readbyqxmd.com/read/28188034/follistatin-like-1-expression-is-decreased-in-the-alveolar-epithelium-of-hypoplastic-rat-lungs-with-nitrofen-induced-congenital-diaphragmatic-hernia
#9
Toshiaki Takahashi, Julia Zimmer, Florian Friedmacher, Prem Puri
BACKGROUND/PURPOSE: Pulmonary hypoplasia (PH), characterized by incomplete alveolar development, remains a major therapeutic challenge associated with congenital diaphragmatic hernia (CDH). Follistatin-like 1 (Fstl1) is a crucial regulator of alveolar formation and maturation, which is strongly expressed in distal airway epithelium. Fstl1-deficient mice exhibit reduced airspaces, impaired alveolar epithelial cell differentiation, and insufficient production of surfactant proteins similar to PH in human CDH...
January 28, 2017: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/28163207/expression-of-recombinant-surfactant-protein-sfta3-in-the-human-kidney-cell-line-hek-293t
#10
H Schröder, R Fischer, L Sollfrank, F Paulsen, L Bräuer, M Schicht
Pulmonary surfactant is broadly known to keep the lung dry, clean and open by lowering the surface tension of the fluid-film that lines the alveoli. The surfactant's protein component, the so called surfactant proteins (SPs), make up a multifunctional protein family. In addition to the four "classical" surfactant proteins (SP-A, SP-B, SP-C and SP-D), which possess immunologic as well as surfactant regulatory properties, two novel putative surfactant proteins (SFTA2 and SFTA3) have recently been described. Neither of them shows sequential nor structural similarity with the already known surfactant proteins...
February 3, 2017: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/28157837/a-novel-surfactant-protein-c-mutation-resulting-in-aberrant-protein-processing-and-altered-subcellular-localization-causes-infantile-interstitial-lung-disease
#11
Da Hong, Yuanyuan Qi, Jing Liu, Huijun Wang, Libo Wang, Liling Qian
BACKGROUND: Mutations in the surfactant protein C gene (SFTPC) result in interstitial lung disease (ILD). Our objective was to report a novel SFTPC mutation and evaluate the effect of this mutant on protein synthesis and processing. METHODS: Genomic DNA was extracted from whole blood of a Chinese infant with ILD and candidate genes associated with ILD were sequenced by next-generation sequencing. Subclones of wild-type and mutant SFTPC were transiently transfected into A549 cells...
February 3, 2017: Pediatric Research
https://www.readbyqxmd.com/read/28157203/hepatocyte-growth-factor-secreted-by-bone-marrow-stem-cell-reduce-er-stress-and-improves-repair-in-alveolar-epithelial-ii-cells
#12
Izabela Nita, Katrin Hostettler, Luca Tamo, Michaela Medová, Giuseppe Bombaci, Jun Zhong, Ramanjaneyulu Allam, Yitzhak Zimmer, Michael Roth, Thomas Geiser, Amiq Gazdhar
Idiopathic Pulmonary Fibrosis (IPF) is a progressive, irreversible lung disease with complex pathophysiology. Evidence of endoplasmic reticulum (ER) stress has been reported in alveolar epithelial cells (AEC) in IPF patients. Secreted mediators from bone marrow stem cells (BMSC-cm) have regenerative properties. In this study we investigate the beneficial effects of BMSC-cm on ER stress response in primary AEC and ER stressed A549 cells. We hypothesize that BMSC-cm reduces ER stress. Primary AEC isolated from IPF patients were treated with BMSC-cm...
February 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28130154/what-killed-karl-patterson-schmidt-combined-venom-gland-transcriptomic-venomic-and-antivenomic-analysis-of-the-south-african-green-tree-snake-the-boomslang-dispholidus-typus
#13
Davinia Pla, Libia Sanz, Gareth Whiteley, Simon C Wagstaff, Robert A Harrison, Nicholas R Casewell, Juan J Calvete
BACKGROUND: Non-front-fanged colubroid snakes comprise about two-thirds of extant ophidian species. The medical significance of the majority of these snakes is unknown, but at least five species have caused life-threatening or fatal human envenomings. However, the venoms of only a small number of species have been explored. METHODS: A combined venomic and venom gland transcriptomic approach was employed to characterise of venom of Dispholidus typus (boomslang), the snake that caused the tragic death of Professor Karl Patterson Schmidt...
April 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28077319/role-of-insulin-like-growth-factor-axis-in-the-bleomycin-induced-lung-injury-in-rats
#14
Lakshmi Kanth Kotarkonda, Ritu Kulshrestha, Krishnan Ravi
BACKGROUND: Alveolar epithelial cell injury has been proposed as a causative factor for the onset and progression of pulmonary fibrosis. However, the role of type II alveolar epithelial cells (AECs) in the epithelial mesenchymal transition (EMT) is controversial. AIMS: The present study performed in rats instilled with bleomycin investigated a) the expressions of the insulin growth factor (IGF-1) and insulin growth factor binding protein 5 (IGFBP-5) and transforming growth factor (TGF-β1) in the type II AECs, b) the role of type II AECs in EMT and extracellular matrix (ECM) formation and, c) the effect of pioglitazone on all the above parameters...
January 7, 2017: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/28071888/synthesis-and-supercapacitor-application-of-alkynyl-carbon-materials-derived-from-cac2-and-polyhalogenated-hydrocarbons-by-interfacial-mechanochemical-reactions
#15
Yingjie Li, Qingnan Liu, Wenfeng Li, Hong Meng, Yingzhou Lu, Chunxi Li
The discovery of new carbon materials and the reactive activation of CaC2 are challenging subjects. In this study, a series of alkynyl carbon materials (ACMs) were synthesized by the interfacial mechanochemical reaction of CaC2 with four typical polyhalogenated hydrocarbons. Their properties and structures were characterized, and their electrochemical performances were examined. The reaction was rapid and efficient arising from the intense mechanical activation of CaC2. The ACMs are micro-mesoporous materials with distinct layered structure, specific graphitization degree, and clear existence of sp-C...
February 1, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28070109/apoptosis-and-surfactant-protein-c-expression-inhibition-induced-by-lipopolysaccharide-in-aec-ii-cell-may-associate-with-nf-%C3%AE%C2%BAb-pathway
#16
Jinle Lin, Jun Tian, Li Wang, Weigang Wu, Huaying Li, Xueyan Wang, Xiaobin Zeng, Wenwu Zhang
Lipopolysaccharide (LPS), a Gram-negative bacterial outer membrane component, is one of the major causes of septic shock. Herein we investigate LPS-induced apoptosis of rat alveolar epithelial type II cells (AEC II) and the effects of LPS on surfactant protein-C (SP-C) expression in AEC II, along with the possible molecular mechanisms. LPS exposure impaired cell viability and increased apoptosis of AEC II significantly in concentration-dependent manner embodied in increased caspase-3 expression and the activity of caspase-3...
2017: Journal of Toxicological Sciences
https://www.readbyqxmd.com/read/28062213/anti-fibrotic-effects-of-bone-morphogenetic-protein-7-modified-bone-marrow-mesenchymal-stem-cells-on-silica-induced-pulmonary-fibrosis
#17
Xiaoli Li, Guoliang An, Yan Wang, Di Liang, Zhonghui Zhu, Ximeng Lian, Piye Niu, Caixia Guo, Lin Tian
Silicosis is an occupational lung disease caused by exposure to small particles of crystalline silica, which ultimately results in diffuse pulmonary fibrosis. Evidence indicates an anti-fibrotic role of bone morphogenetic protein-7 (BMP-7) and bone marrow mesenchymal stem cells (BMSCs) in lung diseases. Therefore, strategies incorporating genetic engineering and stem cell biology might have a tremendous potential to treat critical injuries and diseases. Therefore, we modified BMSCs to overexpress the BMP-7 gene (BMP-7-BMSCs) by lentivirus transduction, and then evaluated whether fibrotic processes were inhibited by these cells in vivo...
January 4, 2017: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/28042067/a-cross-sectional-study-on-the-symptom-burden-of-patients-with-spinal-tumor-validation-of-the-chinese-version-of-the-md-anderson-symptom-inventory-spine-tumor-module
#18
Nanfang Xu, Zhehuang Li, Feng Wei, Xiaoguang Liu, Liang Jiang, Na Meng, Ping Jiang, Miao Yu, Fengliang Wu, Lei Dang, Hua Zhou, Yan Li, Zhongjun Liu
CONTEXT: Tumors involving the spine are associated with unique symptoms affecting both patient survival and health-related quality of life. Currently, there is no disease-specific instrument in Chinese to assess the symptom burden of these patients. OBJECTIVES: The objective of this study was to translate and validate a Chinese version of the MD Anderson Symptom Inventory-Spine Tumor Module (MDASI-SP-C) to assess the symptom burden of Chinese-speaking patients with spinal tumors...
December 29, 2016: Journal of Pain and Symptom Management
https://www.readbyqxmd.com/read/27988781/five-new-species-of-the-i-clubiona-i-i-corticalis-i-species-group-araneae-clubionidae-from-china
#19
Ping Liu, Xianjin Peng, Hengmei Yan
Five new species of the genus Clubiona collected in Mt. Gaoligong, Yunnan Province, China are described: C. biforamina n. sp., C. dactylina n. sp., C. falciforma n. sp., C. multidentata n. sp. and C. tangi n. sp., all belonging to the corticalis species group and known from both males and females.
November 7, 2016: Zootaxa
https://www.readbyqxmd.com/read/27973472/in-vitro-and-in-vivo-comparison-between-poractant-alfa-and-the-new-generation-synthetic-surfactant-chf5633
#20
Francesca Ricci, Xabier Murgia, Roberta Razzetti, Nicola Pelizzi, Fabrizio Salomone
BACKGROUND: CHF5633 is a new generation synthetic surfactant containing both SP-B and SP-C analogues developed for the treatment of respiratory distress syndrome. Here, the optimal dose and its performance in comparison to the animal-derived surfactant poractant alfa were investigated. METHODS: In vitro surfactant activity was determined by means of the Wilhelmy balance and the capillary surfactometer. The dose-finding study was performed in preterm rabbits with severe surfactant deficiency...
February 2017: Pediatric Research
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