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https://www.readbyqxmd.com/read/29044786/unique-profile-of-antimicrobial-peptides-expression-in-polymorphic-light-eruption-lesions-compared-to-healthy-skin-atopic-dermatitis-and-psoriasis
#1
VijayKumar Patra, Gerlinde Mayer, Alexandra Gruber-Wackernagel, Michael Horn, Serena Lembo, Peter Wolf
BACKGROUND: Polymorphic light eruption (PLE) has been attributed to type IV, most likely delayed-type hypersensitivity response (adaptive immunity) but little is known on innate immunity, especially antimicrobial peptides (AMPs) in the disease. Abnormalities in AMP expression have been linked to pathological skin conditions such as atopic dermatitis (AD) and psoriasis. METHODS: AMP profiling was done in PLE skin samples (n,12) compared with that of healthy (n,13), atopic (n,6) and psoriatic skin (n,6)...
October 16, 2017: Photodermatology, Photoimmunology & Photomedicine
https://www.readbyqxmd.com/read/29016111/crystallinity-of-double-stranded-rna-antimicrobial-peptide-complexes-modulates-toll-like-receptor-3-mediated-inflammation
#2
Ernest Y Lee, Toshiya Takahashi, Tine Curk, Jure Dobnikar, Richard L Gallo, Gerard C L Wong
Double-stranded RNA (dsRNA) induces production of pro-inflammatory cytokines in normal human epidermal keratinocytes (NHEK) by specific binding to endosomal Toll-like receptor-3 (TLR3). Recently, it has been shown that hyperactivation of TLR3 in psoriatic keratinocytes by dsRNA can occur in the presence of human antimicrobial peptide (AMP) LL37. Here, we combine synchrotron X-ray scattering, microscopy, computer simulations, and measurements of NHEK cytokine production to elucidate a previously unanticipated form of specific molecular pattern recognition...
October 19, 2017: ACS Nano
https://www.readbyqxmd.com/read/28988712/antimicrobial-peptide-combined-with-bmp2-modified-mesenchymal-stem-cells-promotes-calvarial-repair-in-an-osteolytic-model
#3
Zunpeng Liu, Xue Yuan, Min Liu, Gabriela Fernandes, Yejia Zhang, Shuting Yang, Ciprian N Ionita, Shuying Yang
Repair and regeneration of inflammation-induced bone loss remains a clinical challenge. LL37, an antimicrobial peptide, plays critical roles in cell migration, cytokine production, apoptosis, and angiogenesis. Migration of stem cells to the affected site and promotion of vascularization are essential for tissue engineering therapy, including bone regeneration. However, it is largely unknown whether LL37 affects mesenchymal stem cell (MSC) behavior and bone morphogenetic protein 2 (BMP2)-mediated bone repair during the bone pathologic remodeling process...
September 14, 2017: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/28983954/epr-monitoring-of-wound-oxygenation-as-a-biomarker-of-response-to-gene-therapy-encoding-hcap-18-ll37-peptide
#4
Céline M Desmet, Gaëlle Vandermeulen, Caroline Bouzin, Martin C Lam, Véronique Préat, Philippe Levêque, Bernard Gallez
PURPOSE: To investigate the value of electron paramagnetic resonance oximetry to follow oxygenation in wounds treated by a plasmid-encoding host defense peptide hCAP-18/LL37. METHODS: Flaps were created on diabetic mice (7- or 12-week-old db/db mice) presenting different levels of microangiopathy. The hCAP-18/LL37-encoding plasmids were administered in wounds by electroporation. Low-frequency electron paramagnetic resonance oximetry using lithium phthalocyanine as the oxygen sensor was used to monitor wound oxygenation in flaps during the healing process...
October 5, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/28940795/a-bottom-up-proteomic-approach-to-identify-substrate-specificity-of-outer-membrane-protease-ompt
#5
Sarah E Wood, Gaurav Sinsinbar, Sushanth Gudlur, Madhavan Nallani, Che-Fan Huang, Bo Liedberg, Milan Mrksich
Identifying peptide substrates that are efficiently cleaved by proteases gives insights into substrate recognition and specificity, guides development of inhibitors, and improves assay sensitivity. Peptide arrays and SAMDI mass spectrometry were used to identify a tetrapeptide substrate exhibiting high activity for the bacterial outer-membrane protease (OmpT). Analysis of protease activity for the preferred residues at the cleavage site (P1, P1') and nearest-neighbor positions (P2, P2') and their positional interdependence revealed FRRV as the optimal peptide with the highest OmpT activity...
September 20, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28910369/heightened-circulating-levels-of-antimicrobial-peptides-in-tuberculosis-diabetes-co-morbidity-and-reversal-upon-treatment
#6
Nathella Pavan Kumar, Kadar Moideen, Vijay Viswanathan, Shanmugam Sivakumar, Pradeep A Menon, Hardy Kornfeld, Subash Babu
BACKGROUND: The association of antimicrobial peptides (AMPs) with tuberculosis-diabetes comorbidity (PTB-DM) is not well understood. METHODS: To study the association of AMPs with PTB-DM, we examined the systemic levels of cathelicidin (LL37), human beta defensin- 2 (HBD2), human neutrophil peptides 1-3, (HNP1-3) and granulysin in individuals with either PTB-DM, PTB, latent TB (LTB) or no TB infection (NTB). RESULTS: Circulating levels of cathelicidin and HBD2 were significantly higher and granulysin levels were significantly lower in PTB-DM compared to PTB, LTB or NTB, while the levels of HNP1-3 were significantly higher in PTB-DM compared to LTB or NTB individuals...
2017: PloS One
https://www.readbyqxmd.com/read/28882728/enhanced-efficacy-of-the-engineered-antimicrobial-peptide-wlbu2-via-direct-airway-delivery-in-a-murine-model-of-p-aeruginosa-pneumonia
#7
Chen Chen, Berthony Deslouches, Ronald C Montelaro, Y Peter Di
OBJECTIVES: P. aeruginosa is a common cause of pneumonia in cystic fibrosis patients with the property to generate multidrug resistance against clinically used antibiotics. Antimicrobial peptides (AMPs) are a diverse group of effector molecules of the innate immunity that protect the host against pathogens. However, the lack of activity in common biological matrices has hampered efforts towards clinical development. In this study, we evaluated the therapeutic potential of engineered antimicrobial peptide WLBU2 via direct airway delivery in a murine model of P...
September 4, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28739721/a-preclinical-study-of-cell-seeded-tubularized-scaffolds-specially-secreting-ll37-for-reconstruction-of-long-urethral-defects
#8
Yongwei Li, Jitao Wu, Fan Feng, Changping Men, Diandong Yang, Zhenli Gao, Zhe Zhu, Yuanshan Cui, Hongwei Zhao
AIM: We constructed a new artificial, long tubular acellular matrix, seeded with autologous progenitor cells transfected with the sequence to produce the antibiotic peptide LL37 and another two common seeding cells, which might be adopted for patients requiring repair of long segment of the urethra. MATERIALS AND METHODS: Autologous endothelial progenitor cells transfected by lentiviral vectors expressing antibiotic peptide LL37, as well as urothelial and smooth muscle cells from New Zealand white male rabbits, were cultured and seeded onto preconfigured acellular collagen-based tubular matrices (3 cm in length)...
August 2017: Anticancer Research
https://www.readbyqxmd.com/read/28694030/antimicrobial-peptide-gold-nanoscale-therapeutic-formulation-with-high-skin-regenerative-potential
#9
Michela Comune, Akhilesh Rai, Kiran K Chereddy, Sandra Pinto, Sezin Aday, André F Ferreira, Alessandra Zonari, Josephine Blersch, Rodrigo Cunha, Ricardo Rodrigues, Juan Lerma, Pedro N Simões, Veronique Préat, Lino Ferreira
Chronic skin wounds affect ≈3% of persons aged >60years (Davies et al., 2007) [1]. These wounds are typically difficult to heal by conventional therapies and in many cases they get infected making even harder the regeneration process. The antimicrobial peptide (AMP) LL37 combines antimicrobial with pro-regenerative properties and thus represents a promising topical therapy to address both problems. Here, we investigated the wound healing potential of soluble and immobilized LL37 (LL37-conjugated gold nanoparticles, LL37-Au NPs), both in vitro (migration of keratinocytes) and in vivo (skin wound healing)...
July 8, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28666755/activity-of-innate-antimicrobial-peptides-and-ivacaftor-against-clinical-cystic-fibrosis-respiratory-pathogens
#10
Joanna E Payne, Alice V Dubois, Rebecca J Ingram, Sinead Weldon, Clifford C Taggart, J Stuart Elborn, Michael M Tunney
There is a clear need for new antimicrobials to improve current treatment of chronic lung infection in people with cystic fibrosis (CF). This study determined the activities of antimicrobial peptides (AMPs) and ivacaftor, a novel CF transmembrane conductance regulator potentiator, for CF treatment. Antimicrobial activities of AMPs [LL37, human β-defensins (HβD) 1-4 and SLPI] and ivacaftor against clinical respiratory isolates (Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus spp., Achromobacter spp...
June 27, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28584210/atopic-dermatitis-like-graft-versus-host-disease-and-lichen-planus-like-graft-versus-host-disease-alterations-in-skin-barrier-function-and-related-molecules
#11
Kun Li, Zhang-Lei Mu, Xue Chen, Guang-Dong Wen, Yan Zhao, Jian-Zhong Zhang
BACKGROUND: Graft-versus-host disease (GVHD) is a common complication of hematopoietic stem cell transplantation. Skin barrier disruption could induce thymic stromal lymphopoietin (TSLP) expression, and the expression of TSLP was increased in lesions of atopic dermatitis (AD)-like GVHD and lichen planus (LP)-like GVHD. This study attempted to investigate the skin barrier function of AD-like GVHD and LP-like GVHD and possible mechanisms. METHODS: Eighteen AD-like GVHD patients, 12 LP-like GVHD patients, and 14 healthy volunteers were enrolled in this study...
June 20, 2017: Chinese Medical Journal
https://www.readbyqxmd.com/read/28482118/autoantigens-adamtsl5-and-ll37-are-significantly-upregulated-in-active-psoriasis-and-localized-with-keratinocytes-dendritic-cells-and-other-leukocytes
#12
Judilyn Fuentes-Duculan, Kathleen M Bonifacio, Jason E Hawkes, Norma Kunjravia, Inna Cueto, Xuan Li, Juana Gonzalez, Sandra Garcet, James G Krueger
Psoriasis is a common immune-mediated disease that affects 2%-4% of individuals in North America and Europe. In the past decade, advances in research have led to an improved understanding of immune pathways involved in the pathogenesis of psoriasis and has spurred the development of targeted therapeutics. Recently, three psoriasis autoantigens have been described: cathelicidin (LL37), a disintegrin and metalloprotease domain containing thrombospondin type 1 motif-like 5 (ADAMTSL5), and lipid antigens generated by phospholipase A2 (PLA2) group IVD (PLA2G4D)...
November 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28460048/role-of-pattern-recognition-receptors-in-the-modulation-of-antimicrobial-peptide-expression-in-the-corneal-epithelial-innate-response-to-f-solani
#13
Satya Sree Kolar, Hasna Baidouri, Alison M McDermott
Purpose: Fusarium solani (F. solani) keratitis is a potentially sight-threatening fungal infection of the cornea. Antimicrobial peptides (AMPs), such as human β-defensins (hBDs) and cathelicidins, essential components of the immune system, likely have a protective role against F. solani keratitis. We examined the role of pattern recognition receptors (PRRs), Dectin-1, and TLR2 in F. solani-induced modulation of AMP expression in vitro. Methods: Human corneal epithelial cells (HCECs) were exposed to heat-inactivated F...
May 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28445757/action-of-antimicrobial-peptides-on-bacterial-and-lipid-membranes-a-direct-comparison
#14
COMPARATIVE STUDY
Joseph E Faust, Pei-Yin Yang, Huey W Huang
The bacterial membrane represents an attractive target for the design of new antibiotics to combat widespread bacterial resistance. Understanding how antimicrobial peptides (AMPs) and other membrane-active agents attack membranes could facilitate the design of new, effective antimicrobials. Despite intense study of AMPs on model membranes, we do not know how well the mechanism of attack translates to real biological membranes. To that end, we have characterized the attack of AMPs on Escherichia coli cytoplasmic membranes and directly compared this action to model membranes...
April 25, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28340683/trpv4-moves-toward-center-fold-in%C3%A2-rosacea-pathogenesis
#15
Yong Chen, Carlene D Moore, Jennifer Y Zhang, Russell P Hall, Amanda S MacLeod, Wolfgang Liedtke
Mascarenhas et al. report that TRPV4 expression is upregulated in mast cells in response to the proteolytic cathelicidin fragment LL37 in a murine rosacea model and that TRPV4 loss of function attenuates mast cell degranulation. These findings render TRPV4 a translational-medical target in rosacea. However, signaling mechanisms causing increased expression of TRPV4 await elucidation. Moreover, we ask whether TRPV4-mediated Ca(++)-influx evokes mast cell degranulation.
April 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28328161/synthesis-in-vitro-evaluation-and-68-ga-radiolabeling-of-cdp1-toward-pet-ct-imaging-of-bacterial-infection
#16
Jyotibon Dutta, Sooraj Baijnath, Anou M Somboro, Savania Nagiah, Fernando Albericio, Beatriz G de la Torre, Biljana Marjanovic-Painter, Jan Rijn Zeevaart, Mike Sathekge, Hendrik G Kruger, Anil Chuturgoon, Tricia Naicker, Thomas Ebenhan, Thavendran Govender
Bacterial infections are a major concern in the human health sector due to poor diagnosis and development of multidrug-resistant strains. PET/CT provides a means for the non-invasive detection and localization of the infectious foci; however, the radiotracers available are either cumbersome to prepare or their exact contribution toward the imaging is not yet established. Human antimicrobial peptides are of interest for development as PET radiotracers as they are an integral component of the immune system, non-immunogenic toward the recipient, and show selectivity toward pathogens such as bacteria...
October 2017: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/28326169/circulating-ll37-targets-plasma-extracellular-vesicles-to-immune-cells-and-intensifies-beh%C3%A3-et-s-disease-severity
#17
Tamer Kahraman, Gozde Gucluler, Ismail Simsek, Fuat Cem Yagci, Muzaffer Yildirim, Can Ozen, Ayhan Dinc, Mayda Gursel, Lolai Ikromzoda, Tolga Sutlu, Stephen Gay, Ihsan Gursel
Behçet's disease (BD) activity is characterised by sustained, over-exuberant immune activation, yet the underlying mechanisms leading to active BD state are poorly defined. Herein, we show that the human cathelicidin derived antimicrobial peptide LL37 associates with and directs plasma extracellular vesicles (EV) to immune cells, thereby leading to enhanced immune activation aggravating BD pathology. Notably, disease activity was correlated with elevated levels of circulating LL37 and EV plasma concentration...
2017: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/28220120/neutrophil-extracellular-traps-and-its-implications-in-inflammation-an-overview
#18
REVIEW
Vidal Delgado-Rizo, Marco A Martínez-Guzmán, Liliana Iñiguez-Gutierrez, Alejandra García-Orozco, Anabell Alvarado-Navarro, Mary Fafutis-Morris
In addition to physical barriers, neutrophils are considered a part of the first line of immune defense. They can be found in the bloodstream, with a lifespan of 6-8 h, and in tissue, where they can last up to 7 days. The mechanisms that neutrophils utilize for host defense are phagocytosis, degranulation, cytokine production, and, the most recently described, neutrophil extracellular trap (NET) production. NETs are DNA structures released due to chromatin decondensation and spreading, and they thus occupy three to five times the volume of condensed chromatin...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28178179/diminished-antimicrobial-peptide-and-antifungal-antibiotic-activities-against-candida-albicans-in-denture-adhesive
#19
Amber M Bates, Jorge L Garaicoa, Carol L Fischer, Kim A Brogden
The underlying causes of denture stomatitis may be related to the long-term use of adhesives, which may predispose individuals to oral candidiasis. In this study, we hypothesize that antimicrobial peptides and antifungal antibiotics have diminished anti-Candida activities in denture adhesive. To show this, nine antimicrobial peptides and five antifungal antibiotics with and without 1.0% denture adhesive were incubated with Candida albicans strains ATCC 64124 and HMV4C in radial diffusion assays. In gels with 1...
February 6, 2017: Antibiotics
https://www.readbyqxmd.com/read/28017866/collagen-tethering-of-synthetic-human-antimicrobial-peptides-cathelicidin-ll37-and-its-effects-on-antimicrobial-activity-and-cytotoxicity
#20
Lindsay D Lozeau, Jonian Grosha, Denis Kole, Fioleda Prifti, Tanja Dominko, Terri A Camesano, Marsha W Rolle
Wound infections, particularly of chronic wounds, pose a substantial challenge for designing antimicrobial dressings that are both effective against pathogens, and do not interfere with wound healing. Due to their broad-spectrum antimicrobial and immunomodulatory activities, naturally-occurring antimicrobial peptides (AMPs) are promising alternative treatments. However, their cytotoxicity at high concentrations and poor stability hinders their clinical use. To mitigate these undesirable properties, we investigated the effects of tethering human AMP cathelicidin LL37 to collagen, one of the main extracellular matrix proteins in wound sites, secreted by fibroblasts, and in commercially-available wound dressings...
April 1, 2017: Acta Biomaterialia
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