keyword
https://read.qxmd.com/read/37882966/a-convenient-self-removing-affinity-tag-method-for-the-simple-purification-of-tagless-recombinant-proteins
#1
JOURNAL ARTICLE
Sai Vivek Prabhala, Sophia A Mayone, Nathan M Moody, Chidinma B Kanu, David W Wood
In this work, we describe a novel self-cleaving affinity tag technology based on a highly modified split-intein cleaving element. In this system, which has recently been commercialized by Protein Capture Science, LLC under the name iCapTagTM , the N-terminal segment of an engineered split intein is covalently immobilized onto a capture resin, while the smaller C-terminal intein segment is fused to the N-terminus of the desired target protein. The tagged target can then be expressed in an appropriate expression system, without concern for premature intein cleaving...
October 2023: Current protocols
https://read.qxmd.com/read/37134165/hominin-fossils-from-kromdraai-and-drimolen-inform-paranthropus-robustus-craniofacial-ontogeny
#2
JOURNAL ARTICLE
José Braga, Bernard A Wood, Veronika A Zimmer, Benjamin Moreno, Catherine Miller, John F Thackeray, Bernhard Zipfel, Frederick E Grine
Ontogeny provides critical information about the evolutionary history of early hominin adult morphology. We describe fossils from the southern African sites of Kromdraai and Drimolen that provide insights into early craniofacial development in the Pleistocene robust australopith Paranthropus robustus . We show that while most distinctive robust craniofacial features appear relatively late in ontogeny, a few do not. We also find unexpected evidence of independence in the growth of the premaxillary and maxillary regions...
May 3, 2023: Science Advances
https://read.qxmd.com/read/35470841/highly-fluorescent-bodipy-functionalised-metallacages-as-drug-delivery-systems-synthesis-characterisation-and-cellular-accumulation-studies
#3
JOURNAL ARTICLE
Brech Aikman, Riccardo Bonsignore, Ben Woods, Daniel Doellerer, Riccardo Scotti, Claudia Schmidt, Alexandra A Heidecker, Alexander Pöthig, Edward J Sayers, Arwyn T Jones, Angela Casini
With the aim of designing new metallosupramolecular architectures for drug delivery, research has focused on porous 3-dimensional (3D)-metallacages able to encapsulate cytotoxic agents protecting them from metabolism while targeting them to cancer sites. Here, two self-assembled [Pd2 L4 ]4+ cages (CG1 and CG2) featuring 3,5-bis(3-ethynylpyridine)phenyl ligands (L) exo -functionalised with dipyrromethene (BODIPY) groups have been synthesised and characterised by different methods, including NMR spectroscopy and mass spectrometry...
May 17, 2022: Dalton Transactions: An International Journal of Inorganic Chemistry
https://read.qxmd.com/read/33211120/health-risk-assessment-composition-and-distribution-of-polycyclic-aromatic-hydrocarbons-pahs-in-drinking-water-of-southern-jharkhand-east-india
#4
JOURNAL ARTICLE
Balram Ambade, Shrikanta Shankar Sethi, Amit Kumar, Tapan Kumar Sankar, Sudarshan Kurwadkar
The studies on polycyclic aromatic hydrocarbons (PAHs) occurrence, distribution, health risk, and composition in drinking water are limited in India and worldwide. The main objective of this study was to find the contaminant sources, composition, health risk, and distribution of USEPA's 16 priority pollutant PAHs in the drinking water samples collected between July 2019 to September 2019 from six districts of Southern Jharkhand. The Σ16PAHs mean ± standard deviation [SD] concentration values were ordered as East Singhbhum (ES) (21...
November 19, 2020: Archives of Environmental Contamination and Toxicology
https://read.qxmd.com/read/31492934/in-vitro-and-in-vivo-evaluation-of-the-anticancer-and-anti-inflammatory-activities-of-2-himachelen-7-ol-isolated-from-cedrus-libani
#5
JOURNAL ARTICLE
Andree Elias, Wassim N Shebaby, Bilal Nehme, Wissam Faour, Bassem S Bassil, Joelle El Hakim, Rita Iskandar, Nahia Dib-Jalbout, Mohamad Mroueh, Costantine Daher, Robin I Taleb
Cedrus libani is a majestic evergreen tree native to the Mediterranean mountains of Lebanon, Syria and Turkey. In this study, the tree heart wood was extracted using hexane to produce C. libani oil extract (CLOE) as a dark oil. GCMS analysis of CLOE identified up to 30 compounds whereby 2-himachalen-7-ol (7-HC) was the most abundant (40%). 7-HC was isolated using column chromatography and the identity of the white crystalline solid was confirmed via NMR spectroscopy and X-Ray Crystallography. 7-HC demonstrated potent cytotoxic activity against several human cancer cell lines including brain (SF-268, IC50 8...
September 6, 2019: Scientific Reports
https://read.qxmd.com/read/30966502/column-free-purification-methods-for-recombinant-proteins-using-self-cleaving-aggregating-tags
#6
JOURNAL ARTICLE
Yamin Fan, Jackelyn M Miozzi, Samuel D Stimple, Tzu-Chiang Han, David W Wood
Conventional column chromatography processes to purify recombinant proteins are associated with high production costs and slow volumetric throughput at both laboratory and large scale. Non-chromatographic purifications based on selective aggregating tags have the potential to reduce costs with acceptable protein yields. A significant drawback, however, is that current proteolytic approaches for post-purification tag removal after are expensive and non-scalable. To address this problem, we have developed two non-chromatographic purification strategies that use either the elastin-like polypeptide (ELP) tag or the β-roll tag (BRT17) in combination with an engineered split intein for tag removal...
April 25, 2018: Polymers
https://read.qxmd.com/read/30135101/morphological-intelligence-counters-foot-slipping-in-the-desert-locust-and-dynamic-robots
#7
JOURNAL ARTICLE
Matthew A Woodward, Metin Sitti
During dynamic terrestrial locomotion, animals use complex multifunctional feet to extract friction from the environment. However, whether roboticists assume sufficient surface friction for locomotion or actively compensate for slipping, they use relatively simple point-contact feet. We seek to understand and extract the morphological adaptations of animal feet that contribute to enhancing friction on diverse surfaces, such as the desert locust ( Schistocerca gregaria ) [Bennet-Clark HC (1975) J Exp Biol 63:53-83], which has both wet adhesive pads and spines...
September 4, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://read.qxmd.com/read/28843001/cell-free-production-of-a-therapeutic-protein-expression-purification-and-characterization-of-recombinant-streptokinase-using-a-cho-lysate
#8
JOURNAL ARTICLE
Kevin Tran, Chandrasekhar Gurramkonda, Merideth A Cooper, Manohar Pilli, Joseph E Taris, Nicholas Selock, Tzu-Chiang Han, Michael Tolosa, Adil Zuber, Chariz Peñalber-Johnstone, Christina Dinkins, Niloufar Pezeshk, Yordan Kostov, Douglas D Frey, Leah Tolosa, David W Wood, Govind Rao
The use of cell-free systems to produce recombinant proteins has grown rapidly over the past decade. In particular, cell-free protein synthesis (CFPS) systems based on mammalian cells provide alternative methods for the production of many proteins, including those that contain disulfide bonds, glycosylation, and complex structures such as monoclonal antibodies. In the present study, we show robust production of turbo green fluorescent protein (tGFP) and streptokinase in a cell-free system using instrumented mini-bioreactors for highly reproducible protein production...
January 2018: Biotechnology and Bioengineering
https://read.qxmd.com/read/27472394/c-cbl-regulates-melanoma-proliferation-migration-invasion-and-the-fak-src-grb2-nexus
#9
JOURNAL ARTICLE
Minakshi Nihal, Gary S Wood
Melanoma is one of the most aggressive and lethal forms of skin cancer. Despite recent improvements in targeted therapies, many patients with advanced disease fail to achieve lasting tumor regression. Therefore, it is important to develop novel druggable targets that can be exploited to improve clinical outcome. Here, we studied the role of Casitas B-lineage lymphoma (c-CBL), an E3 ubiquitin ligase, in human melanoma. Employing quantitative real-time PCR and Western blot analysis in a panel of human melanoma cell lines (A375, G361, Hs-294T, SK-Mel-2, SK-Mel-28 and 451Lu), we found that c-CBL is strongly expressed in human melanoma cells at the mRNA and protein levels...
August 16, 2016: Oncotarget
https://read.qxmd.com/read/26662220/a-photoactivatable-platinum-iv-anticancer-complex-conjugated-to-the-rna-ligand-guanidinoneomycin
#10
JOURNAL ARTICLE
Evyenia Shaili, Marta Fernández-Giménez, Savina Rodríguez-Astor, Albert Gandioso, Lluís Sandín, Carlos García-Vélez, Anna Massaguer, Guy J Clarkson, Julie A Woods, Peter J Sadler, Vicente Marchán
A photoactivatable platinum(IV) complex, trans,trans,trans-[Pt(N3 )2 (OH)(succ)(py)2 ] (succ=succinylate, py=pyridine), has been conjugated to guanidinoneomycin to study the effect of this guanidinum-rich compound on the photoactivation, intracellular accumulation and phototoxicity of the pro-drug. Surprisingly, trifluoroacetic acid treatment causes the replacement of an azido ligand and the axial hydroxide ligand by trifluoroacetate, as shown by NMR spectroscopy, MS and X-ray crystallography. Photoactivation of the platinum-guanidinoneomycin conjugate in the presence of 5'-guanosine monophosphate (5'-GMP) led to the formation of trans-[Pt(N3 )(py)2 (5'-GMP)](+) , as does the parent platinum(IV) complex...
December 7, 2015: Chemistry: a European Journal
https://read.qxmd.com/read/25947177/an-integrin-targeted-photoactivatable-pt-iv-complex-as-a-selective-anticancer-pro-drug-synthesis-and-photoactivation-studies
#11
JOURNAL ARTICLE
Albert Gandioso, Evyenia Shaili, Anna Massaguer, Gerard Artigas, Alejandro González-Cantó, Julie A Woods, Peter J Sadler, Vicente Marchán
A new anticancer agent based on the conjugation of a photoactivatable Pt(IV) pro-drug to a cyclic RGD-containing peptide is described. Upon visible light irradiation, phototoxicity was induced preferentially in SK-MEL-28 melanoma cancer cells overexpressing αVβ3 integrin compared to control DU-145 human prostate carcinoma cells.
June 4, 2015: Chemical Communications: Chem Comm
https://read.qxmd.com/read/25798135/diversity-of-thermophiles-in-a-malaysian-hot-spring-determined-using-16s-rrna-and-shotgun-metagenome-sequencing
#12
JOURNAL ARTICLE
Chia Sing Chan, Kok-Gan Chan, Yea-Ling Tay, Yi-Heng Chua, Kian Mau Goh
The Sungai Klah (SK) hot spring is the second hottest geothermal spring in Malaysia. This hot spring is a shallow, 150-m-long, fast-flowing stream, with temperatures varying from 50 to 110°C and a pH range of 7.0-9.0. Hidden within a wooded area, the SK hot spring is continually fed by plant litter, resulting in a relatively high degree of total organic content (TOC). In this study, a sample taken from the middle of the stream was analyzed at the 16S rRNA V3-V4 region by amplicon metagenome sequencing. Over 35 phyla were detected by analyzing the 16S rRNA data...
2015: Frontiers in Microbiology
https://read.qxmd.com/read/23473430/arsenic-toxicity-in-the-human-nerve-cell-line-sk-n-sh-in-the-presence-of-chromium-and-copper
#13
JOURNAL ARTICLE
Ligang Hu, Justin B Greer, Helena Solo-Gabriele, Lynne A Fieber, Yong Cai
As, Cr, and Cu represent one potential combination of multiple metals/metalloids exposures since these three elements are simultaneously leached from chromated copper arsenate (CCA)-treated wood, a common product used for building construction, at levels that can be potentially harmful. This study investigated the neurotoxicity of As associated with CCA-treated wood when accompanied by Cr and Cu. The toxicity was evaluated on the basis of a cytotoxicity model using human neuroblastoma cell line SK-N-SH. The cells were cultured with CCA-treated wood leachates or with solutions containing arsenate [As(V)], divalent copper [Cu(II)], trivalent chromium [Cr(III)] alone or in different combinations of the three elements...
May 2013: Chemosphere
https://read.qxmd.com/read/22768128/anti-neoplastic-activity-of-two-flavone-isomers-derived-from-gnaphalium-elegans-and-achyrocline-bogotensis
#14
JOURNAL ARTICLE
Christan M Thomas, Robert C Wood, Jarrett E Wyatt, Morgan H Pendleton, Ruben D Torrenegra, Oscar E Rodriguez, Sam Harirforoosh, Maria Ballester, Janet Lightner, Koyamangalath Krishnan, Victoria P Ramsauer
Over 4000 flavonoids have been identified so far and among these, many are known to have antitumor activities. The basis of the relationships between chemical structures, type and position of substituent groups and the effects these compounds exert specifically on cancer cells are not completely elucidated. Here we report the differential cytotoxic effects of two flavone isomers on human cancer cells from breast (MCF7, SK-BR-3), colon (Caco-2, HCT116), pancreas (MIA PaCa, Panc 28), and prostate (PC3, LNCaP) that vary in differentiation status and tumorigenic potential...
2012: PloS One
https://read.qxmd.com/read/22484929/cytotoxic-steroids-from-the-trunk-of-berberis-koreana
#15
JOURNAL ARTICLE
Ki Hyun Kim, Sang Un Choi, Chung Sub Kim, Kang Ro Lee
A new steroid, itesmol 3-O-palmitate (1), along with two known steroids were isolated from the trunk of Berberis koreana. The structure of 1 was determined on the basis of spectroscopic analyses involving 2D NMR and chemical reactions. Compound 1 exhibited potential antiproliferative activity against A549, SK-OV-3, SK-MEL-2, and HCT-15 cell lines (respective IC(50) values of 7.41, 9.20, 4.53, and 12.91 µM).
2012: Bioscience, Biotechnology, and Biochemistry
https://read.qxmd.com/read/21569410/a-hepatoprotective-lindera-obtusiloba-extract-suppresses-growth-and-attenuates-insulin-like-growth-factor-1-receptor-signaling-and-nf-kappab-activity-in-human-liver-cancer-cell-lines
#16
JOURNAL ARTICLE
Christian Freise, Martin Ruehl, Ulrike Erben, Ulf Neumann, Daniel Seehofer, Ki Young Kim, Wolfram Trowitzsch-Kienast, Thorsten Stroh, Martin Zeitz, Rajan Somasundaram
BACKGROUND: In traditional Chinese and Korean medicine, an aqueous extract derived from wood and bark of the Japanese spice bush Lindera obtusiloba (L.obtusiloba) is applied to treat inflammations and chronic liver diseases including hepatocellular carcinoma. We previously demonstrated anti-fibrotic effects of L.obtusiloba extract in hepatic stellate cells. Thus, we here consequently examine anti-neoplastic effects of L.obtusiloba extract on human hepatocellular carcinoma (HCC) cell lines and the signaling pathways involved...
May 12, 2011: BMC Complementary and Alternative Medicine
https://read.qxmd.com/read/20688834/suppression-of-colitis-driven-colon-cancer-in-mice-by-a-novel-small-molecule-inhibitor-of-sphingosine-kinase
#17
JOURNAL ARTICLE
Alexander A Chumanevich, Deepak Poudyal, Xiangli Cui, Tia Davis, Patricia A Wood, Charles D Smith, Lorne J Hofseth
Sphingolipid metabolism is driven by inflammatory cytokines. These cascade of events include the activation of sphingosine kinase (SK), and subsequent production of the mitogenic and proinflammatory lipid sphingosine 1-phosphate (S1P). Overall, S1P is one of the crucial components in inflammation, making SK an excellent target for the development of new anti-inflammatory drugs. We have recently shown that SK inhibitors suppress colitis and hypothesize here that the novel SK inhibitor, ABC294640, prevents the development of colon cancer...
October 2010: Carcinogenesis
https://read.qxmd.com/read/16338199/guggul-for-hyperlipidemia-a-review-by-the-natural-standard-research-collaboration
#18
REVIEW
Catherine Ulbricht, Ethan Basch, Philippe Szapary, Paul Hammerness, Serguei Axentsev, Heather Boon, David Kroll, Levi Garraway, Mamta Vora, Jen Woods
OBJECTIVE: To evaluate the scientific evidence on guggul for hyperlipidemia including expert opinion, folkloric precedent, history, pharmacology, kinetics/dynamics, interactions, adverse effects, toxicology, and dosing. METHODS: Electronic searches were conducted in nine databases, 20 additional journals (not indexed in common databases), and bibliographies from 50 selected secondary references. No restrictions were placed on language or quality of publications...
December 2005: Complementary Therapies in Medicine
https://read.qxmd.com/read/16185670/neuronal-prostaglandin-endoperoxide-synthase-2-responses-to-oxygen-and-glucose-deprivation-are-mediated-by-mitogen-activated-protein-kinase-erk1-2
#19
JOURNAL ARTICLE
Daying Zhang, Charles E Wood
Prostanoids in the central nervous system define an important linkage between blood pressure and hormonal responses to hypotension/ischemia. Prostaglandin endoperoxide synthase (PGHS)-2, the inducible isoform of this enzyme, is induced by cerebral hypoperfusion/ischemia. To investigate the mechanism of the PGHS-2 gene expression in response to cerebral hypoperfusion/ischemia in neurons, we used a cell culture model (human SK-N-AS cells) to mimic the oxygen and glucose deprivation (OGD) that usually results from ischemia...
October 26, 2005: Brain Research
https://read.qxmd.com/read/14089496/an-in-vitro-study-of-fibrinolytic-agents-on-v2-carcinoma-cells-and-intravascular-thrombi-in-rabbits
#20
JOURNAL ARTICLE
J H JOHNSON, J R WOODS
No abstract text is available yet for this article.
December 1963: Bulletin of the Johns Hopkins Hospital
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