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https://www.readbyqxmd.com/read/27913096/a-sensitive-and-accurate-lc-ms-ms-assay-with-the-derivatization-of-1amino4methylpiperazine-applied-to-serum-allopregnanolone-pregnenolone-and-androsterone-in-pre-and-postmenopausal-women
#1
Yuyong Ke, Renaud Gonthier, Fernand Labrie
The concentrations of allopregnanolone (Allopreg), pregnenolone (Preg) and androsterone (ADT) are very low in the circulation, especially in postmenopausal women, resulting in a considerable challenge for their accurate measurements in serum or plasma. In this report, a sensitive and reliable LC-MS/MS assay method has been developed using a simple sample preparation and the 1-Amino-4-methylpiperazine (AMP) derivatization procedure. A 5pg/ml (0.1pg on column) of low limit of quantitation has been achieved for Allopreg, Preg and ADT, with a sensitivity comparable to data obtained with the commercial reagent...
November 29, 2016: Steroids
https://www.readbyqxmd.com/read/27908495/analysis-of-low-abundant-trehalose-6-phosphate-and-related-metabolites-in-medicago-truncatula-by-hydrophilic-interaction-liquid-chromatography-triple-quadrupole-mass-spectrometry
#2
Ana Teresa Mata, Tiago Filipe Jorge, João Ferreira, Maria do Rosário Bronze, Diana Branco, Pedro Fevereiro, Susana Araújo, Carla António
Trehalose-6-phosphate (T6P) is an important signaling metabolite involved in plant growth control that inhibits the sucrose nonfermenting-1-related protein kinase 1 (SnRK1), a key regulator of energy and carbon metabolism in plants. The quantification of T6P in plant tissues is fundamental to improve our understanding of sugar signaling and the links between plant growth and development in response to stress conditions. However, the almost undetectable levels of T6P together with the complex plant matrix and the presence of T6P isomers such as sucrose-6-phosphate (S6P), makes the detection of this metabolite challenging...
November 21, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/27907131/itraq-based-quantitative-proteomic-comparison-of-early-and-late-passage-human-dermal-papilla-cell-secretome-in-relation-to-inducing-hair-follicle-regeneration
#3
Huan Zhang, Ning-Xia Zhu, Keng Huang, Bo-Zhi Cai, Yang Zeng, Yan-Ming Xu, Yang Liu, Yan-Ping Yuan, Chang-Min Lin
Alopecia is an exceedingly prevalent problem that lacks effective therapy. Recently, research has focused on early-passage dermal papilla cells (DPCs), which have hair inducing activity both in vivo and in vitro. Our previous study indicated that factors secreted from early-passage DPCs contribute to hair follicle (HF) regeneration. To identify which factors are responsible for HF regeneration and why late-passage DPCs lose this potential, we collected 48-h-culture medium (CM) from both of passage 3 and 9 DPCs, and subcutaneously injected the DPC-CM into NU/NU mice...
2016: PloS One
https://www.readbyqxmd.com/read/27907059/quantitative-and-selective-analysis-of-feline-growth-related-proteins-using-parallel-reaction-monitoring-high-resolution-mass-spectrometry
#4
Mårten Sundberg, Emma M Strage, Jonas Bergquist, Bodil S Holst, Margareta Ramström
Today immunoassays are widely used in veterinary medicine, but lack of species specific assays often necessitates the use of assays developed for human applications. Mass spectrometry (MS) is an attractive alternative due to high specificity and versatility, allowing for species-independent analysis. Targeted MS-based quantification methods are valuable complements to large scale shotgun analysis. A method referred to as parallel reaction monitoring (PRM), implemented on Orbitrap MS, has lately been presented as an excellent alternative to more traditional selected reaction monitoring/multiple reaction monitoring (SRM/MRM) methods...
2016: PloS One
https://www.readbyqxmd.com/read/27898991/nipple-and-areola-sparing-mastectomy-for-the-treatment-of-breast-cancer
#5
REVIEW
Bruna S Mota, Rachel Riera, Marcos Desidério Ricci, Jessica Barrett, Tiago B de Castria, Álvaro N Atallah, Jose Luiz B Bevilacqua
BACKGROUND: The efficacy and safety of nipple-sparing mastectomy and areola-sparing mastectomy for the treatment of breast cancer are still questionable. It is estimated that the local recurrence rates following nipple-sparing mastectomy are very similar to breast-conserving surgery followed by radiotherapy. OBJECTIVES: To assess the efficacy and safety of nipple-sparing mastectomy and areola-sparing mastectomy for the treatment of ductal carcinoma in situ and invasive breast cancer in women...
November 29, 2016: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/27898364/liquid-chromatography-tandem-mass-spectrometry-assay-for-the-quantification-of-niraparib-and-its-metabolite-m1-in-human-plasma-and-urine
#6
L van Andel, Z Zhang, S Lu, V Kansra, S Agarwal, L Hughes, M M Tibben, A Gebretensae, H Rosing, J H M Schellens, J H Beijnen
Niraparib (MK-4827) is a novel poly(ADP-Ribose) polymerase (PARP) inhibitor currently investigated in phase III clinical trials to treat cancers. The development of a new drug includes the characterisation of absorption, metabolism and excretion (AME) of the compound. AME studies are a requirement of regulatory agencies and for this purpose bioanalytical assays are essential. This article describes the development and validation of a bioanalytical assay for niraparib and its carboxylic acid metabolite M1 in human plasma and urine using liquid chromatography-tandem mass spectrometry (LC-MS/MS)...
November 19, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/27892962/peptides-labeled-with-pyridinium-salts-for-sensitive-detection-and-sequencing-by-electrospray-tandem-mass-spectrometry
#7
Mateusz Waliczek, Monika Kijewska, Magdalena Rudowska, Bartosz Setner, Piotr Stefanowicz, Zbigniew Szewczuk
Mass spectrometric analysis of trace amounts of peptides may be problematic due to the insufficient ionization efficiency resulting in limited sensitivity. One of the possible ways to overcome this problem is the application of ionization enhancers. Herein we developed new ionization markers based on 2,4,6-triphenylpyridinium and 2,4,6-trimethylpyridinium salts. Using of inexpensive and commercially available pyrylium salt allows selective derivatization of primary amino groups, especially those sterically unhindered, such as ε-amino group of lysine...
November 28, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27875788/simultaneous-determination-and-pharmacokinetic-study-of-four-phenolic-acids-in-rat-plasma-using-uflc-ms-ms-after-intravenous-administration-of-salvianolic-acid-for-injection
#8
Xiuman Xie, Jingzhuo Miao, Wanyang Sun, Jingyi Huang, Dongxiang Li, Shuming Li, Ling Tong, Guoxiang Sun
A simple, sensitive and selective ultra-fast liquid chromatography-tandem mass spectrometry (UFLC-MS/MS) method was established for simultaneous determination and pharmacokinetic study of rosmarinic acid (RA), salvianolic acid D (Sal D), lithospermic acid (LA) and salvianolic acid B (Sal B) in rat plasma after intravenous administration of salvianolic acid for injection (SAFI). Three doses of administration, containing 14, 28 and 56mg/kg, were investigated in this study. Plasma samples were pretreated using protein precipitation (PP) with pre-cooled acetonitrile...
November 5, 2016: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/27875787/simultaneous-determination-of-anemoside-b4-phellodendrine-berberine-palmatine-obakunone-esculin-esculetin-in-rat-plasma-by-uplc-esi-ms-ms-and-its-application-to-a-comparative-pharmacokinetic-study-in-normal-and-ulcerative-colitis-rats
#9
Lianrong Yang, Xin Meng, Xiaojin Yu, Haixue Kuang
A sensitive and rapid ultra-performance liquid chromatography-electrospray ionization-mass spectrometry (UPLC-ESI-MS/MS) method was developed for the simultaneous analysis of anemoside B4, phellodendrine, berberine, palmatine, obakunone, esculin, esculetin, toosendanin (IS1 of anemoside B4), tetrahydropalmatine (IS2 of phellodendrine, berberine, palmatine and obakunone) and scopoletin (IS3 of esculin and esculetin) and to compare the pharmacokinetics of these active ingredients in normal and ulcerative colitis rats...
November 11, 2016: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/27867277/characterization-and-bioavailability-study-of-baicalin-mesoporous-carbon-nanopowder-solid-dispersion
#10
Li Cui, E Sune, Jie Song, Jing Wang, Xiao-Bin Jia, Zhen-Hai Zhang
BACKGROUND: Baicalin is the main bioactive constitute of the dried roots of Scutellaria baicalensis and possesses various biological activities. However, the poor water solubility and low oral bioavailability limit its efficacy. OBJECTIVE: The present study was conducted to enhance the dissolution and oral bioavailability of baicalin (BA) through a novel mesoporous carbon nanopowder (MCN) drug carrier. MATERIALS AND METHODS: Solid dispersions (SDs) of BA with MCN were prepared using a solvent evaporation method...
October 2016: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/27865793/multiplexed-mass-spectrometry-monitoring-of-biomarker-candidates-for-osteoarthritis
#11
Patricia Fernández-Puente, Valentina Calamia, Lucía González-Rodríguez, Lucía Lourido, María Camacho-Encina, Natividad Oreiro, Cristina Ruiz-Romero, Francisco J Blanco
: The methods currently available for the diagnosis and monitoring of osteoarthritis (OA) are very limited and lack sensitivity. Being the most prevalent rheumatic disease, one of the most disabling pathologies worldwide and currently untreatable, there is a considerable interest pointed in the verification of specific biological markers for improving its diagnosis and disease progression studies. Considering the remarkable development of targeted proteomics methodologies in the frame of the Human Proteome Project, the aim of this work was to develop and apply a MRM-based method for the multiplexed analysis of a panel of 6 biomarker candidates for OA encoded by the Chromosome 16, and another 8 proteins identified in previous shotgun studies as related with this pathology, in specimens derived from the human joint and serum...
November 16, 2016: Journal of Proteomics
https://www.readbyqxmd.com/read/27861246/adrenal-venous-sampling-in-primary-aldosteronism-multinomial-regression-modeling-to-detect-aldosterone-secretion-lateralization-when-right-adrenal-sampling-is-missing
#12
Camille Durivage, Rémi Blanchette, Gilles Soulez, Miguel Chagnon, Patrick Gilbert, Marie-France Giroux, Isabelle Bourdeau, Vincent L Oliva, André Lacroix, Eric Therasse
OBJECTIVE: Difficulty to recognize or canulate the right adrenal vein is the most frequent cause of adrenal venous sampling (AVS) failure. We aimed to assess multinomial regression modeling (MRM) of peripheral and left adrenal vein samplings to detect lateralization of aldosterone secretion when the right AVS is missing. METHODS: Simultaneous bilateral AVS samplings were performed before (basal) and after intravenous cosyntropin injection in 188 consecutive patients between December 1989 and September 2015...
November 16, 2016: Journal of Hypertension
https://www.readbyqxmd.com/read/27846433/validated-quantification-method-for-five-ephedrines-in-dietary-supplements-using-lc-ms-ms-application-to-503-cases
#13
Jianli Zhang, Yinong Zhang, Yan Wang
Ephedrines in dietary supplements can arise from herbs or illegal adulteration so a liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed for separation and quantification of ephedrine, pseudoephedrine, norephedrine, norepseudoephedrine, and methyl-ephedrine, some of which are isomer pairs of pseudo-structures. This method includes liquid-liquid extraction of ephedrines from three typical dietary supplement matrices-solid, liquid, and oil-as well as liquid chromatographic separation...
November 9, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/27845782/a-targeted-proteomic-multiplex-csf-assay-identifies-increased-malate-dehydrogenase-and-other-neurodegenerative-biomarkers-in-individuals-with-alzheimer-s-disease-pathology
#14
R W Paterson, W E Heywood, A J Heslegrave, N K Magdalinou, U Andreasson, E Sirka, E Bliss, C F Slattery, J Toombs, J Svensson, P Johansson, N C Fox, H Zetterberg, K Mills, J M Schott
Alzheimer's disease (AD) is the most common cause of dementia. Biomarkers are required to identify individuals in the preclinical phase, explain phenotypic diversity, measure progression and estimate prognosis. The development of assays to validate candidate biomarkers is costly and time-consuming. Targeted proteomics is an attractive means of quantifying novel proteins in cerebrospinal and other fluids, and has potential to help overcome this bottleneck in biomarker development. We used a previously validated multiplexed 10-min, targeted proteomic assay to assess 54 candidate cerebrospinal fluid (CSF) biomarkers in two independent cohorts comprising individuals with neurodegenerative dementias and healthy controls...
November 15, 2016: Translational Psychiatry
https://www.readbyqxmd.com/read/27842983/a-novel-hplc-mrm-strategy-to-discover-unknown-and-long-term-metabolites-of-stanozolol-for-expanding-analytical-possibilities-in-doping-control
#15
Zhe Wang, Xinmiao Zhou, Xin Liu, Ying Dong, Jinlan Zhang
Stanozolol is one of the most commonly abused anabolic androgenic steroids (AAS) by athletes and usually detected by its parent drug and major metabolites. However, its metabolic pathway is complex, varied and individually different, it is important to characterize its overall metabolic profiles and discover new and long-term metabolites for the aims of expanding detection windows. High performance liquid chromatography coupled with triple quadrupole mass spectrometer (HPLC-MS/MS) was used to analyze the human urine after oral administration of stanozolol...
November 8, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/27834935/simultaneous-determination-of-coumarin-and-its-derivatives-in-tobacco-products-by-liquid-chromatography-tandem-mass-spectrometry
#16
Zhiqin Ren, Bo Nie, Tong Liu, Fei Yuan, Feng Feng, Yuan Zhang, Weie Zhou, Xiuli Xu, Meiyi Yao, Feng Zhang
In this paper an analytical method based on high performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) for the determination of coumarin and its derivatives in tobacco products was developed. The MS/MS fragmentation pathways of the eight coumarins were elucidated. The new analytical method was defined based on two main axes, an extraction procedure with acetonitrile and analyte detection performed by HPLC-MS/MS in electron impact mode. The excellent selectivity and sensitivity achieved in multiple reaction monitoring (MRM) mode allowed satisfactory confirmation and quantitation for the coumarin flavor additives...
November 10, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27826630/infliximab-quantitation-in-human-plasma-by-liquid-chromatography-tandem-mass-spectrometry-towards-a-standardization-of-the-methods
#17
Jean-Francois Jourdil, Dorothée Lebert, Elodie Gautier-Veyret, Florian Lemaitre, Bruno Bonaz, Guillaume Picard, Julia Tonini, Françoise Stanke-Labesque
Infliximab (IFX) is a chimeric monoclonal antibody targeting tumor necrosis factor-alpha. It is currently approved for the treatment of certain rheumatic diseases or inflammatory bowel diseases. Clinical studies have suggested that monitoring IFX concentrations could improve treatment response. However, in most studies, IFX was quantified using ELISA assays, the resulting discrepancies of which raised concerns about their reliability. Here, we describe the development and validation of a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for IFX quantification in human plasma...
November 8, 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27821331/micron-sized-surface-enhanced-raman-scattering-reporter-fluorescence-probe-encoded-colloidal-microspheres-for-sensitive-dna-detection
#18
Lijun You, Ruimin Li, Xu Dong, Fang Wang, Jia Guo, Changchun Wang
A new type of optical probes, featuring surface enhanced Raman scattering (SERS) and fluorescence spectra dual-mode encoding, has been reported in this article. Based on the uniform micrometer-sized melamine resin/Ag nanoparticles (MRM/Ag-NPs) composite microspheres, the SERS reporters and fluorescent probes were successfully fixed onto the different layers of the MEM/Ag-NPs microspheres, which supported the sensitive DNA detecton. The two spectroscopic methods commonly considered to be contradictive to each other, yet the optical signals were separable in the experiments...
October 31, 2016: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/27805616/a-high-throughput-multiplexed-and-targeted-proteomic-csf-assay-to-quantify-neurodegenerative-biomarkers-and-apolipoprotein-e-isoforms-status
#19
Wendy E Heywood, Anna Baud, Emily Bliss, Ernestas Sirka, Jonathan M Schott, Henrik Zetterberg, Daniela Galimberti, Neil J Sebire, Kevin Mills
Many neurodegenerative diseases are still lacking effective treatments. Reliable biomarkers for identifying and classifying these diseases will be important in the development of future novel therapies. Often potential new biomarkers do not make it into the clinic due to limitations in their development and high costs. However, targeted proteomics using Multiple Reaction Monitoring Liquid Chromatography-tandem/Mass Spectrometry (MRM LC-MS/MS), specifically using triple quadrupole mass spectrometers, is one method that can be used to rapidly evaluate and validate biomarkers for clinical translation into diagnostic laboratories...
October 20, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27802918/high-throughput-assay-for-quantification-of-the-plasma-concentrations-of-thiopental-using-automated-solid-phase-extraction-spe-directly-coupled-to-lc-ms-ms-instrumentation
#20
Seyed Mojtaba Moosavi, Kiran Shekar, John Fraser, Maree T Smith, Sussan Ghassabian
Most previous assays for thiopental are time-consuming due to laborious sample extraction steps prior to analysis using gas chromatography or high pressure liquid chromatography. Here, we describe the first high-throughput liquid chromatography - tandem mass spectrometry (LC-MS/MS) method for quantification of thiopental concentrations in samples of human plasma. Robotic on-line solid phase extraction (SPE) was used to elute the analytes of interest from samples of human plasma (50μL) loaded onto C18 SPE cartridges to which were added aliquots (50μL) of internal standard solution (thiopental-d5 100ng/mL) and 0...
October 27, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
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