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https://www.readbyqxmd.com/read/28539714/evaluation-of-herb-drug-interactions-of-hovenia-dulcis-fruit-extracts
#1
Jong Suk Park, Shaheed Ur Rehman, In Sook Kim, Min Sun Choi, Chun-Soo Na, Hye Hyun Yoo
BACKGROUND: Hovenia dulcis (Rhamnaceae) fruits are popularly used as herbal medicines or dietary supplements in Asian countries due to functions such as liver protection and detoxification from alcohol poisoning. Accordingly, it is very likely for dietary supplemental products, including H. dulcis fruit extracts, to be taken with prescription drugs. OBJECTIVE: In this study, possible food-drug interactions involving H. dulcis fruit extracts were evaluated based on the inhibition of cytochrome P450 (CYP) enzyme activity...
April 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28539059/phenolics-from-castanea-sativa-leaves-and-their-effects-on-uvb-induced-damage
#2
Antonietta Cerulli, Milena Masullo, Angela Mari, Anna Balato, Rosanna Filosa, Serena Lembo, Assunta Napolitano, Sonia Piacente
The phytochemical investigation of the methanol extract of the leaves of Castanea sativa Mill., source of the Italian PGI (Protected Geographical Indication) product 'Marrone di Roccadaspide' (Campania region) afforded as main compounds crenatin (1), chestanin (2), gallic acid (3), cretanin (4), 5-O-p-coumaroylquinic acid (5), p-methylgallic acid (6) and quercetin-3-O-glucoside (7). To quantify the isolated compounds a LC-ESI(QqQ)MS method working with a very sensitive and selective mass tandem experiment called Multiple Reaction Monitoring (MRM) has been developed...
May 25, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28532827/hepatoma-derived-growth-factor-protein-quantification-in-uterine-fluid-gene-expression-in-endometrial-cell-culture-and-effects-on-in%C3%A2-vitro-embryo-development-pregnancy-and-birth
#3
E Gómez, S Carrocera, D Martin, M J Sánchez-Calabuig, A Gutiérrez-Adán, A Murillo, M Muñoz
Hepatoma-derived growth factor (HDGF) is present in the endometrium of cows and other mammals. Recombinant HDGF (rHDGF) improves bovine blastocyst development in vitro. However, specific culture conditions and essential aspects of HDGF uterine physiology are yet unknown. In this work we quantified total HDGF protein in uterine fluid (UF) by multiple reaction monitoring (MRM), and analyzed effects of rHDGF on specific embryonic stages with Day-6 bovine embryos cultured in vitro with and without BSA, and on pregnancy viability and calf phenotypes after embryo transfer to recipients...
July 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28530406/comparative-evaluation-of-small-molecular-additives-and-their-effects-on-peptide-protein-identification
#4
Jing Gao, Shaoyun Zhong, Yanting Zhou, Han He, Shuying Peng, Zhenyun Zhu, Xing Liu, Jing Zheng, Bin Xu, Hu Zhou
Detergents and salts are widely used in lysis buffers to enhance protein extraction from biological samples, facilitating in-depth proteomic analysis. However, these detergents and salt additives must be efficiently removed from the digested samples prior to LC-MS/MS analysis to obtain high-quality mass spectra. Although filter-aided sample preparation (FASP), acetone precipitation (AP), followed by in-solution digestion, and strong cation exchange-based centrifugal proteomic reactors (CPRs) are commonly used for proteomic sample processing, little is known about their efficiencies at removing detergents and salt additives...
May 22, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28521250/development-and-validation-of-lc-ms-ms-methods-for-the-measurement-of-ribociclib-a-cdk4-6-inhibitor-in-mouse-plasma-and-ringer-s-solution-and-its-application-to-a-cerebral-microdialysis-study
#5
Ashish Kala, Yogesh T Patel, Abigail Davis, Clinton F Stewart
LC-MS/MS methods to measure ribociclib in mouse plasma and Ringer's solution were successfully developed and validated. Reverse phase chromatography was performed with gradient elution using C18 (100A, 50×4.6mm, 3μ) and C8-A (50×2.0mm, 5μ) columns for plasma and Ringer's samples, respectively. Mouse plasma samples were extracted using solid phase extraction method, whereas no extraction was required for the Ringer's solution samples. Analytes were detected using positive ion MRM mode. The precursor to product ions (Q1→Q3) selected for ribociclib and d6-ribociclib were (m/z) 435...
July 1, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28516774/multiplexed-mrm-based-protein-quantitation-using-two-different-stable-isotope-labeled-peptides-for-calibration
#6
André LeBlanc, Sarah A Michaud, Andrew J Percy, Darryl B Hardie, Juncong Yang, Nicholas J Sinclair, Jillaine I Proudfoot, Adam Pistawka, Derek S Smith, Christoph H Borchers
Precise and robust quantitation of the endogenous plasma proteome is a requirement for fundamental and biomedical research as well as for clinical applications. Targeted detection of peptides in a bottom-up strategy is the most common and precise mass spectrometry-based quantitation approach when combined with the use of stable isotope labeled peptides. However, when measuring protein in plasma, the unknown endogenous levels prevent the implementation of best calibration strategies since no blank matrix is available...
May 18, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28511951/proteome-profiling-reveals-immune-responses-in-japanese-flounder-paralichthys-olivaceus-infected-with-edwardsiella-tarda-by-itraq-analysis
#7
Lei Wang, Changwei Shao, Wenteng Xu, Qian Zhou, Na Wang, Songlin Chen
The liver is an important organ for bacterial pathogen attack in fish. The differential proteomic response of the Japanese flounder liver to Edwardsiella tarda infection was examined using isobaric tags for relative and absolute quantitation (iTRAQ) labeling followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). A total of 3290 proteins were identified and classified into categories related to biological process (51.4%), molecular function (63.6%), and cellular component (57.7%). KEGG enrichment analysis indicated the complement and coagulation cascade pathways and the mineral absorption pathway were significantly enriched...
May 13, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28502009/quantitative-mass-spectrometry-by-isotope-dilution-and-multiple-reaction-monitoring-mrm
#8
Paul Russo, Brian L Hood, Nicholas W Bateman, Thomas P Conrads
Selected reaction monitoring (SRM) is used in molecular profiling to detect and quantify specific known proteins in complex mixtures. Using isotope dilution (Barnidge et al., Anal Chem 75(3):445-451, 2003) methodologies, peptides can be quantified without the need for an antibody-based method. Selected reaction monitoring assays employ electrospray ionization mass spectrometry (ESI-MS) followed by two stages of mass selection: a first stage where the mass of the peptide ion is selected and, after fragmentation by collision-induced dissociation (CID), a second stage (tandem MS) where either a single (e...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28501205/an-integrated-strategy-for-the-quantitative-analysis-of-endogenous-proteins-a-case-of-gender-dependent-expression-of-p450-enzymes-in-rat-liver-microsome
#9
Yuhao Shao, Xiaoxi Yin, Dian Kang, Boyu Shen, Zhangpei Zhu, Xinuo Li, Haofeng Li, Lin Xie, Guangji Wang, Yan Liang
Liquid chromatography mass spectrometry based methods provide powerful tools for protein analysis. Cytochromes P450 (CYPs), the most important drug metabolic enzymes, always exhibit sex-dependent expression patterns and metabolic activities. To date, analysis of CYPs based on mass spectrometry is still facing critical technical challenges due to the complexity and diversity of CYP isoforms besides lack of corresponding standards. The aim of present work consisted in developing a label-free qualitative and quantitative strategy for endogenous proteins, and then applying to the gender-difference study for CYPs in rat liver microsomes (RLMs)...
August 1, 2017: Talanta
https://www.readbyqxmd.com/read/28493441/uhplc-esi-ms-ms-determination-and-pharmacokinetics-of-pinoresinol-glucoside-and-chlorogenic-acid-in-rat-plasma-after-oral-administration-of-eucommia-ulmoides-oliv-extract
#10
Xiaojian Gong, Qingxiang Luan, Xin Zhou, Yang Zhao, Chao Zhao
This study aimed to develop a specific UHPLC-ESI-MS/MS method for simultaneous determination and pharmacokinetics of pinoresinol glucoside and chlorogenic acid in rat plasma after oral administration of E. ulmoides. The chromatographic separation was achieved on a Hypersil GOLD column with gradient elution by using a mixture of 0.1% formic acid aqueous solution and acetonitrile as the mobile phase at a flow rate of 200 μL/min. A tandem mass spectrometric detection was conducted using multiple-reaction monitoring (MRM) via an electrospray ionization (ESI) source in negative ionization mode...
May 10, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28493402/development-and-validation-of-hplc-ms-ms-method-for-the-determination-of-lixivaptan-in-mouse-plasma-and-its-application-in-a-pharmacokinetic-study
#11
Adnan A Khady, Haithem Alrabiah, Mohamed W Attwa, Sabray Attia, Gamal A E Mostafa
The aim of the present study was to develop a simple, sensitive, and accurate liquid chromatography-electrospray ionization tandem mass spectrometry (ESI-MS/MS) method for the determination of lixivaptan (LIX) in mouse plasma by using vildagliptin as the internal standard (IS). A precipitation procedure was used for the extraction of LIX and vildagliptin from mouse plasma. Chromatographic separation of LIX was achieved using a C18 analytical column (50 mm × 2.1 mm, 1.8 µm) at 25 °C. The mobile phase comprised of acetonitrile and ammonium formate (10 mM, pH 3...
May 10, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28490445/serological-epithelial-component-proteins-identify-intestinal-complications-in-crohn-s-disease
#12
Yunki Y Yau, Rupert Wl Leong, Aviv Pudipeddi, Diane Redmond, Valerie C Wasinger
Crohns Disease (CD) is a relapsing inflammation of the gastrointestinal tract that affects a young working age population and is increasing in developing countries. Half of all sufferers will experience stricturing or fistulizing intestinal complications that require extensive surgical interventions and neither genes nor clinical risk factors can predict this debilitating natural history. We applied discovery and verification phase studies as part of an NCI-FDA modelled biomarker pipeline to identify differences in the low-mass (<25kDa) blood-serum proteome between CD behavioural phenotypes...
May 10, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28488747/uplc-mrm-ms-quantification-of-trastuzumab-in-human-serum-by-selective-monitoring-of-a-specific-peptide-marker-from-the-antibody-complementarity-determining-regions
#13
R Russo, C Rega, A Caporale, G Tonon, S Scaramuzza, F Selis, M Ruvo, A Chambery
RATIONALE: Because of the large molecular weight, of the structural complexity and the similarity with endogenous immunoglobulins present in high concentrations, in vivo quantitative studies with therapeutic monoclonal antibodies are particularly challenging. In this work, an UPLC/MRM MS-based methodology is described for the quantification of Trastuzumab in human serum by monitoring a novel specific peptide marker located within its heavy chain Complementarity-Determining Region (CDR)...
May 10, 2017: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/28488287/design-for-the-transfer-of-a-validated-lc-ms-ms-analytical-method-for-the-determination-of-antimicrobial-residues-in-honey-from-low-resolution-to-high-resolution-mass-spectrometry
#14
Khaled E L Hawari, Mohamad A L Iskandarani, Sophie Mompelat, Dominique Hurtaud-Pessel, Eric Verdon
RATIONALE: This paper investigates the validity of the transfer of a Liquid Chromatography tandem Mass Spectrometry (LC-MS/MS) method for the determination of veterinary medicinal products in honey and compared to an LC- Linear Ion Trap/Orbitrap Mass Spectrometry method. Descriptive statistical approach was performed in order to assess if such transfer may succeed or fail. This approach is based on the simultaneous evaluation of the trueness and of the intermediate precision for each compound at a 95% interval of confidence of both analytical techniques...
May 10, 2017: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/28485147/pharmacokinetics-of-cajaninstilbene-acid-and-its-main-glucuronide-metabolite-in-rats
#15
Li-Sha Wang, Xue Tao, Rui-Le Pan, Fang-Rui Cao, Li Feng, Yong-Hong Liao, Xin-Min Liu, Qi Chang
As a major active stilbene from the leaves of pigeon pea (Cajanus cajan), cajaninstilbene acid (CSA) exerts various pharmacological activities. The present study aimed to investigate the pharmacokinetics of CSA and one of its main metabolites (M1) to explore their fate in the body and provide a pharmacokinetic foundation for their in vivo biological activities and functional food or complementary medicine application. M1 was characterized as CSA-3-O-glucuronide using the multiple reaction monitoring-information-dependent acquisition-enhanced product ion technique...
May 16, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28480861/development-of-the-g-c-m-s-m-s-method-for-qualitative-and-quantitative-determination-of-clozapine-in-human-blood
#16
K Sivsivadze, M Jokhadze, P Tushurashvili, T Murtazashvili, N Imnadze
A simple, rapid, selective, sensitive gas chromatography-mass spectrometry (GC-MS/MS) method was developed and validated for the determination of clozapine in human blood. For isolation was used solid phase extraction. The calibration standards range was 5-1500ng/mL. Analysis was carried out on a gas chromatograph Agilent 7000A Quadrupole GC-MS/MS. GC run conditions - capillary column HP-5MS, 30m × 0.25mm, coated with a 0.25μm film, column temperature from 50°C to 305°C with an increase of 10°C/min; the injection port and the transfer line temperature were 310°C; carrier gas - helium at flow rate of 1...
March 2017: Georgian Medical News
https://www.readbyqxmd.com/read/28477121/peptide-immunoaffinity-enrichment-with-targeted-mass-spectrometry-application-to-quantification-of-atm-kinase-phospho-signaling
#17
Jeffrey R Whiteaker, Lei Zhao, Regine M Schoenherr, Jacob J Kennedy, Richard G Ivey, Amanda G Paulovich
Peptide immunoaffinity enrichment coupled with targeted mass spectrometry is a quantitative approach for the robust and reproducible quantification of peptide analytes. The approach is capable of multiplexed quantification of peptides, including posttranslational modifications such as phosphorylation. Anti-peptide antibodies are used to enrich analytes and heavy stable isotope-labeled standards. The enriched peptides are directly measured by multiple reaction monitoring (MRM), a well-characterized quantitative mass spectrometry-based method...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28467948/integrating-uhplc-ms-ms-quantification-and-das-analysis-to-investigate-the-effects-of-wine-processing-on-the-tissue-distributions-of-bioactive-constituents-of-herbs-in-rats-exemplarily-shown-for-dipsacus-asper
#18
Yi Tao, Yingshan Du, Weidong Li, Baochang Cai, Liuqing Di, Liyun Shi, Lihong Hu
Wine-processing, which is sauteing with rice wine, will change the inclination and direction of herbs' actions. After being wine-processed, the effects of nourishing liver and kidney of Dipsacus asper will be strengthened. However, the underlying mechanism remains elusive. The following study is to establish and validate an UHPLC-MS/MS approach to determine six bioactive constituents in tissue samples, including loganin, loganic acid, chlorogenic acid, 3,5-dicaffeoylquinic acid, 4-caffeoylquinic acid and asperosaponin VI and apply the approach to a comparative tissue distribution study of raw and wine-processed Dipsacus asper in rats...
April 25, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28463725/screening-allergic-components-of-yejuhua-injection-using-lad2-cell-membrane-chromatography-model-online-with-high-performance-liquid-chromatography-ion-trap-time-of-flight-mass-spectrum-system
#19
Yanni Lv, Jia Fu, Xianpeng Shi, Zhiwei Yang, Shengli Han
Yejuhua (YJH) injection is a traditional Chinese medicine (TCM) injection and has a widely application in clinical practice. However, adverse drug reactions (ADRs) caused by YJH injection, majorly manifested as allergic reactions, have been reported. Hence, Effective and practical method for allergen screening and identification is needed. In this work, a LAD2/CMC model coupled online with HPLC-IT-TOF-MS system was developed to screen, analyze, and identify the allergenic components of YJH injection. A fraction was retained on the LAD2/CMC column, and identified as linarin (LN)...
April 27, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28463158/postmastectomy-radiation-therapy-is-associated-with-improved-survival-in-node-positive-male-breast-cancer-a-population-analysis
#20
Matthew J Abrams, Paul P Koffer, David E Wazer, Jaroslaw T Hepel
PURPOSE: Because of its rarity, there are no randomized trials investigating postmastectomy radiation therapy (PMRT) in male breast cancer. This study retrospectively examines the impact of PMRT in male breast cancer patients in the National Cancer Institute's Surveillance, Epidemiology, and End Results (SEER) database. METHODS AND MATERIALS: The SEER database 8.3.2 was queried for men ages 20+ with a diagnosis of localized or regional nonmetastatic invasive ductal/lobular carcinoma from 1998 to 2013...
June 1, 2017: International Journal of Radiation Oncology, Biology, Physics
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