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https://www.readbyqxmd.com/read/28922609/serum-proteomic-variability-associated-with-clinical-phenotype-in-familial-transthyretin-amyloidosis-attrm
#1
Gloria G Chan, Clarissa M Koch, Lawreen H Connors
Transthyretin (TTR), normally a plasma circulating protein, can become misfolded and aggregated, ultimately leading to extracellular deposition of amyloid fibrils usually targeted to heart or nerve tissues. Referred to as TTR-associated amyloidoses (ATTR), this group of diseases is frequently life threatening and fatal if untreated. ATTR, caused by amyloid-forming variant TTR proteins (ATTRm) which arise from point mutations in the TTR gene, were classically referred to as familial amyloid cardiomyopathy (FAC) or familial amyloid polyneuropathy (FAP) reflecting the clinical phenotype...
September 18, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28920441/new-insight-of-common-regulatory-pathways-in-human-trabecular-meshwork-cells-in-response-to-dexamethasone-and-prednisolone-using-an-integrated-quantitative-proteomics-swath-and-mrm-hr-mass-spectrometry
#2
Sze Wan Shan, Chi Wai Do, Thomas Chuen Lam, Ricky Pak Wing Kong, King Kit Li, Ka Man Chun, William Daniel Stamer, Chi Ho To
The molecular pathophysiology of corticosteroid-induced ocular hypertension (CIH) is not well understood. To determine the biological mechanisms of CIH, this study investigated protein expression profiles of human trabecular meshwork (hTM) cells in response to dexamethasone and prednisolone treatment. Both discovery-based Sequential Windowed data independent Acquisition of the Total High-resolution mass spectra (SWATH(TM)-MS) and targeted based high resolution multiple reaction monitoring (MRM-HR) confirmation were applied using a hybrid quadrupole-time-of-flight mass spectrometer...
September 18, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28918325/a-novel-lc-ms-ms-method-for-the-quantitative-measurement-of-the-acetate-content-in-pharmaceutical-peptides
#3
Rani J Qasem, Ibrahim K Farh, Mohammed A Al Essa
Most pharmaceutical peptides are supplied as acetate salts and the relative amount of acetate to peptide in the final product is one quality criterion required by regulatory agencies to approve the product for clinical use. The objective of the present study was to develop a validated LC-MS/MS method that allows the quantitative determination of the acetate content in pharmaceutical peptide preparations and simultaneous monitoring and collection of qualitative and quantitative information on the peptide during manufacture and in the final product...
September 6, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28917164/uplc-ms-ms-assay-of-riluzole-in-human-plasma-and-cerebrospinal-fluid-csf-application-in-samples-from-spinal-cord-injured-patients
#4
Mahua Sarkar, Robert G Grossman, Elizabeth G Toups, Diana S-L Chow
In the present study, a sensitive and robust LC-MS/MS method has been developed and validated for the quantification of riluzole in human plasma and cerebrospinal fluid (CSF) in clinical samples from patients with spinal cord injury (SCI). Riluzole and its labeled internal standard (IS) were isolated from plasma and CSF by liquid-liquid extraction using ethyl acetate. Riluzole (m/z 235→166) and IS (m/z 238→169) were detected by electrospray ionization (ESI) using multiple reaction monitoring (MRM) in a positive mode...
September 1, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28900116/response-induced-in-mycoplasma-gallisepticum-under-heat-shock-might-be-relevant-to-infection-process
#5
Ivan Butenko, Anna Vanyushkina, Olga Pobeguts, Daria Matyushkina, Sergey Kovalchuk, Alexey Gorbachev, Nicolay Anikanov, Gleb Fisunov, Vadim Govorun
Despite the fact the term "proteome" was proposed to characterize a set of proteins in one of mycoplasma species, proteome response to various exposures in this bacteria are still obscure. Commonly, authors studying proteomic response on perturbation models in mycoplasmas use single approach and do not confirm their findings by alternative methods. Consequently, the results of proteomic analysis should be validated by complementary techniques. In this study we utilized three complementary approaches (SWATH, MRM, 2D-DIGE) to assess response of Mycoplasma gallisepticum under heat stress on proteomic level and combined these findings with metabolic response and the results of transcriptional profiling...
September 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28898764/sensitive-analysis-of-bioactive-secondary-metabolites-in-lichen-species-using-liquid-chromatography-mass-spectrometry
#6
Syed Ghulam Musharraf, Fareeha Siddiqi, Arslan Ali, Vinitha Moolchand Thadhani
Lichens are a large group of valuable lower plants with unique features and diverse applications worldwide such as in medicine, cosmetics, food, and textile industries. They are also well known for their potential in observing climate and environmental monitoring. Their successful exploitations require reliable analytical methods to check and maintain quality and efficacy of the products based on them. This study focuses on the development of a sensitive and reliable quantification method for the analysis of important depsides, depsidones, dibenzofuran and monocyclic phenols inseven known and an unidentified lichen species...
August 13, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28895652/quantitative-proteomics-and-targeted-fatty-acids-analysis-reveal-the-damage-of-triptolide-in-liver-and-kidney
#7
Menglin Li, Ting Hu, Cai Tie, Liang Qu, Hao Zheng, Jinlan Zhang
Triptolide (TP), the major active component in Tripterygium wilfordii Hook. f., is widely used for the treatment of rheumatoid arthritis and autoimmune disease. However, organ toxicity, especially hepatotoxicity and nephrotoxicity, limits its clinical application. To fully understand the mechanism underlying TP toxicity, iTRAQ-based 2D-LC-MS/MS proteomics was used to detect differentially expressed proteins in the livers and kidneys of mice administered the LD50 dose of TP. Functional annotation revealed that multiple pathways are involved in TP toxicity, including acute-phase response signaling, the antigen presentation pathway, FXR/RXR activation, LPS/IL-1-mediated inhibition of RXR function and EIF2 signaling...
September 12, 2017: Proteomics
https://www.readbyqxmd.com/read/28895300/exsta-external-standard-addition-method-for-accurate-high-throughput-quantitation-in-targeted-proteomics-experiments
#8
Yassene Mohammed, Jingxi Pan, Suping Zhang, Jun Han, Christoph H Borchers
PURPOSE: Targeted proteomics using multiple reaction monitoring (MRM) with stable-isotope-labeled internal-standard (SIS) peptides is the current method of choice for protein quantitation in complex biological matrices. Better quantitation can be achieved with the internal standard-addition method, where successive increments of synthesized natural form (NAT) of the endogenous analyte are added to each sample, a response curve is generated, and the endogenous concentration is determined at the x-intercept...
September 11, 2017: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/28895216/metabolic-alterations-in-two-cirsium-species-identified-at-distinct-phenological-stages-using-uplc-qtof-ms
#9
Min-Sun Kim, Miso Nam, Geum-Sook Hwang
INTRODUCTION: Cirsium chanroenicum and C. setidens are commonly used both in traditional folk medicine and as a food source. The quality of different species of Cirsium at different harvest times is a function of their metabolite composition, which is determined by the phenological stage. OBJECTIVE: We sought to determine the differences in the metabolite composition of two species of Cirsium during different phenological stages using ultra-performance liquid chromatography (UPLC) quadrupole time-of-flight (QTOF) mass spectrometry (MS)...
September 12, 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28892901/invasive-ductal-carcinoma-breast-how-neoadjuvant-chemotherapy-affects-the-status-of-estrogen-receptor-progesterone-receptor-and-her2-neu-a-tertiary-care-centre-study
#10
Swasti Shubham, Pratibha Maan, Monika Singh, Minakshi Bhardwaj
INTRODUCTION: Determination of Estrogen Receptor (ER), Progesterone Receptor (PR) and HER2/neu in primary Invasive Ductal Carcinoma (IDC) breast is the standard of care parameter for determining treatment options. Whether or not Neoadjuvant Chemotherapy (NAC) affects the receptor status is still an unanswered question. AIM: To compare immunohistochemical (IHC) profiles of ER, PR and HER2/neu in primary IDC breast before and after NAC to assess the subsequent effects on receptor status...
July 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28890969/triazolium-cyclodextrin-click-cluster-resin-conjugate-an-enrichment-material-for-phosphatidylinositol-3-4-5-triphosphate
#11
H T Le, J W Lee, S C Park, J W Jeong, W Jung, C W Lim, K P Kim, T W Kim
UltraLink was functionalized with a triazolium cyclodextrin click cluster (CCC) which provides a well-oriented, multivalent, positively charged binding site for PtdIns(3,4,5)P3. MALDI TOF MS and LC ESI MS/MS MRM analysis of spiked PtdIns(3,4,5)P3 in lipid extract suggest that triazolium CCC-UltraLink conjugate can be used as an enrichment material for PtdIns(3,4,5)P3.
September 11, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28889219/a-validated-uplc-ms-ms-method-coupled-with-protein-precipitation-and-ion-exchange-solid-phase-extraction-for-the-quantitation-of-porcine-relaxin-b29-in-dog-plasma-and-its-application-to-a-pharmacokinetic-study
#12
Chong Su, Hui Sun, Hong Yang, Lei Yin, Jiwen Zhang, John Paul Fawcett, Jingkai Gu
Porcine relaxin is a 6 kDa peptide hormone of pregnancy with important physiological and pharmacological effects. It contains a number of analogs of which porcine relaxin B29 is one of the most important. To support the development of porcine relaxin B29 as a new drug, we established an UPLC-MS/MS method for its quantitation in dog plasma. Sample preparation by protein precipitation and ion exchange solid phase extraction was followed by UPLC on an XBridge™ BEH300 C18 column at 40 °C in a run time of only 5...
September 9, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28888755/an-atmospheric-pressure-chemical-ionisation-liquid-chromatographic-tandem-mass-spectrometry-method-for-the-analysis-of-benzodiazepines-in-urine
#13
S Dunlop, K Hayes, P Leavy, D Cusack, R Maguire
The objective of this work was to establish an analytical method for the analysis of 7 Benzodiazepines (diazepam, oxazepam, temazepam, nordiazepam, desalkylflurazepam, alprazolam and α-hydroxyalprazolam) in urine specimens taken from drivers suspected of driving under the influence of drugs. The specimen, calibrator and control preparation involved hydrolysis of conjugated benzodiazepines using β-glucuronidase in sodium acetate buffer, with incubation at 60°C for 2h. Specimens were then centrifuged, before being diluted 1 in 5 (total dilution 1 in 10), with 10% acetonitrile in water...
August 24, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28888677/quantitation-of-lanosterol-in-the-vitreous-humor-of-rabbits-after-ocular-administration-of-lanosterol-thermogel-formulation-by-ultra-performance-liquid-chromatography-tandem-mass-spectrometry-with-the-electrospray-ionization-mode
#14
Lei Lv, Dan Li, Hui Wang, Chengjian Li, Xian Qian, Heng-Tao Dong, Jianguo Sun, Liang Zhao
Cataracts are the most common cause of blindness worldwide affecting tens of millions of people. Here, we report a simple, rapid, sensitive and specific method by ultra performance liquid chromatography-tandem mass spectrometry with the electrospray ionization mode (UPLC-ESI-MS/MS) for quantitation of lanosterol, a possible effective drug for cataracts, in the vitreous humor of rabbits after ocular administration. The injected lanosterol was prepared by dispersing lanosterol molecules into the poly-(dl-lactic acid-co-glycolic acid)-poly(ethylene glycol)-poly-(dl-lactic acid-co-glycolic acid) (PLGA-PEG-PLGA) thermogel solution...
September 1, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28887231/therapeutic-activity-of-superoxide-dismutase-containing-enzymosomes-on-rat-liver-ischaemia-reperfusion-injury-followed-by-magnetic-resonance-microscopy
#15
Paulo Marcelino, H Susana Marinho, Maria Celeste Campos, Ana Rita Neves, Carla Real, Filipa S Fontes, Alexandra Carvalho, Gabriel Feio, M Bárbara F Martins, M Luísa Corvo
Liver ischaemia-reperfusion injury (IRI) may occur during hepatic surgery and is unavoidable in liver transplantation. Superoxide dismutase enzymosomes (SOD-enzymosomes), liposomes where SOD is at the liposomal surface expressing enzymatic activity in intact form without the need of liposomal disruption, were developed with the aim of having a better insight into its antioxidant therapeutic outcome in IRI. We also aimed at validating magnetic resonance microscopy (MRM) at 7T as a tool to follow IRI. SOD-enzymosomes were characterized and tested in a rat ischaemia-reperfusion model and the therapeutic outcome was compared with conventional long circulating SOD liposomes and free SOD using biochemical liver injury biomarkers, histology and MRM...
September 6, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28886579/development-validation-and-clinical-pharmacokinetic-application-of-ultra-performance-liquid-chromatography-tandem-mass-spectrometry-method-for-simultaneously-determining-a-novel-recombinant-hirudin-derivative-neorudin-and-its-active-metabolite-in-human-serum
#16
Xiaona Dong, Zhiyun Meng, Jide Jin, Ruolan Gu, Guifang Dou
Recombinant Neorudin (EPR-hirudin, EH), a novel, low-bleeding anticoagulant fusion protein, has been developed as an inactive prodrug that is converted to an active metabolite, hirudin variant 2-Lys47 (HV2), at the thrombus site and is undergoing Phase I clinical trials in China. The goal of our present research was to establish a novel ultra-performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method for simultaneously quantifying EH and HV2 in human serum. Furthermore, the method was used in clinical pharmacokinetic study after validation...
August 24, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28886477/detection-of-8-hydroxydeoxyguanosine-8-ohdg-as-a-biomarker-of-oxidative-damage-in-peripheral-leukocyte-dna-by-uhplc-ms-ms
#17
Danni Wu, Baodong Liu, Junfa Yin, Tian Xu, Shuli Zhao, Qun Xu, Xi Chen, Hailin Wang
8-Hydroxydeoxyguanosine (8-OHdG) is a widely-used biomarker of oxidative DNA damages. 8-OHdG in peripheral blood leukocyte is associated with mutation and cancer risk. The level of 8-OHdG in peripheral blood leukocytes can indicate a long-term response to oxidative stress rather than that in urine. Accurate identification and quantification of leukocyte 8-OHdG are essential for understanding its mechanism of formation, repair, and biological consequences. In this study, a fast and accurate ultra-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed and validated to detect 8-OHdG in human peripheral leukocyte...
August 26, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28880243/simultaneous-determination-of-seven-phenolic-acids-in-rat-plasma-using-uhplc-esi-ms-ms-after-oral-administration-of-echinacea-purpurea-extract
#18
Yan Du, Zhibin Wang, Libo Wang, Mingjie Gao, Liqian Wang, Chunli Gan, Chunjuan Yang
A rapid and sensitive Ultra High Performance Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry (UHPLC-ESI-MS/MS) method was developed and validated to simultaneously determine the concentration of seven phenolic acids (syringic acid, ferulic acid, caffeic acid, vanillic acid, p-coumaric acid, 3,4-dihydroxybenzoic acid and 4-hydroxybenzoic acid) in rat plasma after oral administration of Echinacea purpurea extract. After mixing with the internal standard (IS), butylparaben, plasma samples were prepared by liquid-liquid extraction with ethyl acetate...
September 7, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28880079/quantitative-multiple-reaction-monitoring-proteomic-analysis-of-g%C3%AE-and-g%C3%AE-subunits-in-c57bl6-j-brain-synaptosomes
#19
Yun Young Yim, W Hayes McDonald, Karren Hyde, Osvaldo Cruz-Rodríguez, John J G Tesmer, Heidi E Hamm
Gβγ dimers are one of the essential signaling units of activated G protein coupled receptors (GPCRs). There are five Gβ and twelve Gγ subunits in humans; numerous studies have demonstrated that different Gβ and Gγ subunits selectively interact to form unique Gβγ dimers, which in turn may target specific receptors and effectors. Perturbation of Gβγ signaling can lead to impaired physiological responses. Moreover, previous targeted multiple reaction monitoring (MRM) studies of Gβ and Gγ subunits have shown distinct regional and subcellular localization patterns in four brain regions...
September 7, 2017: Biochemistry
https://www.readbyqxmd.com/read/28878320/a-new-mass-spectrometry-based-method-for-the-quantification-of-histones-in-plasma-from-septic-shock-patients
#20
J L García-Giménez, C Romá-Mateo, N Carbonell, L Palacios, L Peiró-Chova, E García-López, M García-Simón, R Lahuerta, C Gimenez-Garzó, E Berenguer-Pascual, M I Mora, M L Valero, A Alpízar, F J Corrales, J Blanquer, F V Pallardó
The aim of this study was to develop a novel method to detect circulating histones H3 and H2B in plasma based on multiple reaction monitoring targeted mass spectrometry and a multiple reaction monitoring approach (MRM-MS) for its clinical application in critical bacteriaemic septic shock patients. Plasma samples from 17 septic shock patients with confirmed bacteraemia and 10 healthy controls were analysed by an MRM-MS method, which specifically detects presence of histones H3 and H2B. By an internal standard, it was possible to quantify the concentration of circulating histones in plasma, which were significantly higher in patients, and thus confirmed their potential as biomarkers for diagnosing septic shock...
September 6, 2017: Scientific Reports
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