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Zebrafish reproduction

Ana Cecilia Gomes Silva, Daniela Volcan Almeida, Bruna Felix Nornberg, Jessica Ribeiro Pereira, Diego Martins Pires, Carine Dahl Corcini, Antonio Sergio Varela Junior, Luis Fernando Marins
Growth hormone (GH) transgenesis presents a high potential application in aquaculture. However, excess GH may have serious consequences due to pleiotropic actions. In order to study these effects in zebrafish (Danio rerio), two transgenic lines were developed. The first expresses GH ubiquitously and constitutively (F0104 line), while the second expresses the GH receptor in a muscle-specific manner (Myo-GHR line). Results from the F0104 line showed accelerated growth but increased reproductive difficulties, while Myo-GHR did not show the expected increase in muscle mass...
October 14, 2016: Transgenic Research
Charles Teta, Yogeshkumar S Naik
Industrial and municipal effluents regularly pollute water bodies and cause various toxic effects to aquatic life. Because of the diverse nature of industrial processes and domestic products, urban effluents are often tainted with various anthropogenic endocrine disrupting chemicals that may interfere with the reproductive physiology of aquatic fauna. In this study, we tested effluents from the City of Bulawayo for the presence of estrogenic endocrine disrupting chemicals and their effects on fish gonads and fecundity...
October 8, 2016: Chemosphere
Shu-Yan Wu, Li-Dan Wang, Jin-Ling Li, Guang-Mei Xu, Mei-Ling He, Yuan-Yuan Li, Rui Huang
Salmonella enterica serovar typhimurium (S. typhimurium) is globally distributed and causes massive morbidity and mortality in humans and animals. S. typhimurium carries Salmonella plasmid virulence (spv) locus, which is highly conserved and closely related to bacterial pathogenicity, while its exact role in host immune responses during infection remains to be elucidated. To counteract the invaders, the host has evolved numerous strategies, among which the innate immunity and autophagy act as the first defense...
September 22, 2016: Fish & Shellfish Immunology
Yanbin Zhao, Kun Zhang, Karl Fent
Synthetic corticosteroids may pose an environmental risk to fish. Here, we describe multiend point responses of adult zebrafish (8 months old) upon 21-day exposure to a commonly prescribed corticosteroid, fludrocortisone acetate (FLU), at concentrations between 0.006 and 42 μg/L. No remarkable reproductive impacts were observed, while physiological effects, including plasma glucose level and blood leukocyte numbers were significant altered even at 42 ng/L. Ovary parameters and transcriptional analysis of hypothalamic-pituitary-gonadal-liver axis revealed negligible effects...
September 20, 2016: Environmental Science & Technology
Bridget B Baker, Jeremiah S Yee, Danielle N Meyer, Doris Yang, Tracie R Baker
We have shown that zebrafish (Danio rerio) are an excellent model for evaluating the link between early life stage exposure to environmental chemicals and disease in adulthood and subsequent unexposed generations. Previously, we used this model to identify transgenerational effects of dioxin (2,3,7,8-tetrachlorodibenzo-p-dioxin [TCDD]) on skeletal development, sex ratio, and reproductive capacity. Transgenerational inheritance of TCDD toxicity, notably decreased reproductive capacity, appears to be mediated through the male germ line...
October 2016: Zebrafish
Joshua T Gamse, Daniel A Gorelick
For more than 60 years, zebrafish have been used in toxicological studies. Due to their transparency, genetic tractability, and compatibility with high-throughput screens, zebrafish embryos are uniquely suited to study the effects of pharmaceuticals and environmental insults on embryonic development, organ formation and function, and reproductive success. This special issue of Zebrafish highlights the ways zebrafish are used to investigate the toxic effects of endocrine disruptors, pesticides, and heavy metals...
October 2016: Zebrafish
Fangjie Cao, Lizhen Zhu, Hui Li, Song Yu, Chengju Wang, Lihong Qiu
In the past few decades, extensive application of azoxystrobin has led to great concern regarding its adverse effects on aquatic organisms. The objective of the present study was to evaluate the reproductive toxicity of azoxystrobin to zebrafish. After adult zebrafish of both sexes were exposed to 2, 20 and 200 μg/L azoxystrobin for 21 days, egg production, the fertilization rate, the gonadosomatic index (GSI) and hepatosomatic index (HSI), 17β-estradiol (E2), testosterone (T) and vitellogenin (Vtg) concentrations, and histological alterations in the gonads and livers were measured...
September 8, 2016: Environmental Pollution
Pingping Wang, Zhongkun Du, Shixiang Gao, Xiaowei Zhang, J P Giesy
Effects of chemical mixtures at environmentally relevant concentrations on endocrine systems of aquatic organisms are of concern. Triclocarban (TCC) and inorganic mercury (Hg(2+)) are ubiquitous in aquatic environments, and are known to interfere with endocrine pathways via different mechanisms of toxic action. However, effects of mixtures of the two pollutants on aquatic organisms and associated molecular mechanisms were unknown. This study examined effects of binary mixtures of TCC and Hg(2+) on histopathological and biochemical alteration of reproductive organs in zebrafish (Danio rerio) after 21 d exposure...
September 6, 2016: Ecotoxicology and Environmental Safety
Xin Qi, Mohamed Salem, Wenyi Zhou, Miwa Sato-Shimizu, Gang Ye, Johan Smitz, Chun Peng
Neurokinin B (NKB) and its receptor, NK3R, play critical roles in reproduction by regulating the secretion of the hypothalamic GnRH. NKB and NK3R genes are also expressed in the ovary; however, their physiological roles within the ovary are unknown. The aim of this study was to determine whether NKB acts directly on the ovary to regulate reproduction. Injection of NKB into zebrafish accelerated follicle development, increased the mRNA levels of cyp11a1 and cyp19a1, and enhanced estradiol production. Similarly, NKB induced cyp11a1 and cyp19a1 expression in primary cultures of zebrafish follicular cells and stimulated estradiol production from cultured follicles...
September 2016: Endocrinology
Matthew B Tucker, Sarah H MacKenzie, Joseph J Maciag, Hayley Dirscherl Ackerman, Paul Swartz, Jeffrey A Yoder, Paul T Hamilton, A Clay Clark
The regulation of caspase-3 enzyme activity is a vital process in cell fate decisions leading to cell differentiation and tissue development or to apoptosis. The zebrafish, Danio rerio, has become an increasingly popular animal model to study several human diseases because of their transparent embryos, short reproductive cycles, and ease of drug administration. While apoptosis is an evolutionarily conserved process in metazoans, little is known about caspases from zebrafish, particularly regarding substrate specificity and allosteric regulation compared to the human caspases...
August 31, 2016: Protein Science: a Publication of the Protein Society
Yuansi Zheng, Jiebo Lin, Jieyi Li, Haifeng Zhang, Weiming Ai, Xuedong Wang, Randy A Dahlgren, Huili Wang
The toxicity of β-diketone antibiotics (DKAs), a class of ''pseudo-persistent'' environmental pollutants, to F0-zebrafish (Danio rerio) was investigated using 7-dpf F1-zebrafish miRNA sequencing and bioinformatics analyses. Based on relative expression, 47, 134 and 118 of 193 mature miRNAs were differentially expressed between control vs 6.25 mg/L, control vs 12.5 mg/L and 6.25 vs 12.5 mg/L treatments, respectively. Utilizing three databases, 2523 potential target genes were predicted, and they were assigned to 19 high-abundance KEGG pathways and 20 functional categories by COG analysis...
December 2016: Chemosphere
You-Ning Ma, Chu-Yan Cao, Qiang-Wei Wang, Wen-Jun Gui, Guo-Nian Zhu
The widely used organotins have the potential to disrupt the endocrine system, but little is known of underlying mechanisms of azocyclotin toxicity in fish. The objective of the present study was to investigate the impact of azocyclotin on reproduction in zebrafish. Adult zebrafish were exposed to 0.09 and 0.45μg/L azocyclotin for 21days, and effects on steroid hormones and mRNA expression of the genes belonging to the hypothalamic-pituitary-gonad (HPG) axis were investigated. Mass spectrometry methodology was developed to profile steroids within the metabolome of the gonads...
October 2016: Aquatic Toxicology
Jing Ying Hoo, Yatinesh Kumari, Mohd Farooq Shaikh, Seow Mun Hue, Bey Hing Goh
The utilization of zebrafish in biomedical research is very common in the research world nowadays. Today, it has emerged as a favored vertebrate organism for the research in science of reproduction. There is a significant growth in amount numbers of scientific literature pertaining to research discoveries in reproductive sciences in zebrafish. It has implied the importance of zebrafish in this particular field of research. In essence, the current available literature has covered from the very specific brain region or neurons of zebrafish, which are responsible for reproductive regulation, until the gonadal level of the animal...
2016: BioMed Research International
Chiranjib Chakraborty, Ashish Ranjan Sharma, Garima Sharma, Sang-Soo Lee
Presently, nanotechnology is a multi-trillion dollar business sector that covers a wide range of industries, such as medicine, electronics and chemistry. In the current era, the commercial transition of nanotechnology from research level to industrial level is stimulating the world's total economic growth. However, commercialization of nanoparticles might offer possible risks once they are liberated in the environment. In recent years, the use of zebrafish (Danio rerio) as an established animal model system for nanoparticle toxicity assay is growing exponentially...
2016: Journal of Nanobiotechnology
Yali Zhao, Chanpreet Singh, David A Prober, Nancy L Wayne
GnRH neurons integrate internal and external cues to control sexual maturation and fertility. Homeostasis of energy balance and food intake correlates strongly with the status of reproduction. Neuropeptides secreted by the hypothalamus involved in modulating energy balance and feeding may play additional roles in the regulation of reproduction. Hypocretin (Hcrt) (also known as orexin) is one such peptide, primarily controlling sleep/wakefulness, food intake, and reward processing. There is a growing body of evidence indicating that Hcrt/orexin (Hcrt) modulates reproduction through interacting with the hypothalamo-pituitary-gonadal axis in mammals...
October 2016: Endocrinology
Vivian Tang, Zenobia C Cofer, Shuang Cui, Valerie Sapp, Kathleen M Loomes, Randolph P Matthews
OBJECTIVES: Biliary atresia (BA) is a progressive fibroinflammatory cholangiopathy affecting the bile ducts of neonates. Although BA is the leading indication for pediatric liver transplantation, the etiology remains elusive. Adducin 3 (ADD3) and X-prolyl aminopeptidase 1 (XPNPEP1) are 2 genes previously identified in genome-wide association studies as potential BA susceptibility genes. Using zebrafish, we investigated the importance of ADD3 and XPNPEP1 in functional studies. METHODS: To determine whether loss of either gene leads to biliary defects, we performed morpholino antisense oligonucleotide (MO) knockdown studies targeting add3a and xpnpep1 in zebrafish...
November 2016: Journal of Pediatric Gastroenterology and Nutrition
Nana Xu, Pengqian Mu, Zhiqiang Yin, Qi Jia, Shuming Yang, Yongzhong Qian, Jing Qiu
As persistent organic pollutants, polychlorinated biphenyls (PCBs) accumulate in the bodies of animals and humans, resulting in toxic effects on the reproductive, immune, nervous, and endocrine systems. The biological and toxicological characteristics of enantiomers of chiral PCBs may differ, but these enantioselective effects of PCBs have not been fully characterized. In this study, we performed metabolomics analysis, using ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) to investigate the enantioselective toxic effects of PCB95 in zebrafish (Danio rerio) embryos after exposure to three dose levels of 0...
2016: PloS One
Jiangfei Chen, Xiaotong Wang, Xiaoqing Ge, Dingding Wang, Ting Wang, Lingnan Zhang, Robert L Tanguay, Michael Simonich, Changjiang Huang, Qiaoxiang Dong
Perfluorooctanesulphonic acid (PFOS) is a ubiquitous contaminant in the aquatic environment and our earlier studies demonstrated that chronic PFOS exposures lead to a female-biased sex ratio and decreased sperm quality in male zebrafish. The underlying mechanism for these reproductive effects is unknown. In the present study, 8 h post-fertilization (hpf) zebrafish were exposed to PFOS at 250 μg/L for 5 months, and the levels of sex hormones, expression of sex determination related genes, and histological and ultrastructural changes of gonads were fully characterized...
November 2016: Environmental Pollution
Jan L Lyche, Irena M Grześ, Camilla Karlsson, Rasoul Nourizadeh-Lillabadi, Peter Aleström, Erik Ropstad
Apoptosis is an integral element of development that may also be initiated by environmental contaminants. The aim of the present study was to assess potential changes in the regulation of apoptotic genes in zebrafish embryos following parental exposure to two natural mixtures of persistent organic pollutants (POP). The mixture from Lake Mjøsa contained exceptionally high concentrations of polybrominated diphenyl ethers (PBDE), as well as relatively high levels of polychlorinated biphenyls (PCB) and dichlorodiphenyltrichloroethane (DDT)...
2016: Journal of Toxicology and Environmental Health. Part A
Jia-Lang Zheng, Shuang-Shuang Yuan, Wei-Ye Li, Chang-Wen Wu
Certain light emitting diodes (LEDs) have become popular in fish farming beacause of a promoting effect on growth and reproduction. However, little information is available on innate immune responses in related tissues under LEDs conditions. The present study assessed the effects of a white fluorescent bulb (the control) and two different light-emitting diodes (LEDs: blue, LDB, peak at 450 nm; red, LDR, 630 nm) on growth and innate immune responses in the serum, liver and ovary of zebrafish for 8 weeks. LDB significantly enhanced specific growth rate (SGR), food intake (FI), and serum globulin levels...
September 2016: Fish & Shellfish Immunology
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