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Salmonella biofilms

Beatriz Pérez Montoro, Nabil Benomar, Leyre Lavilla Lerma, Sonia Castillo Gutiérrez, Antonio Gálvez, Hikmate Abriouel
A collection of 31 Lactobacillus pentosus strains isolated from naturally fermented Aloreña green table olives were screened in depth in the present study for their probiotic potential. Several strains could be considered promising probiotic candidates since they showed good growth capacity and survival under simulated gastro-intestinal conditions (acidic pH of 1.5, up to 4% of bile salts and 5 mM of nitrate), good ability to auto-aggregate which may facilitate their adhesion to host cells as multiple aggregates and the subsequent displacement of pathogens...
2016: Frontiers in Microbiology
Rui Seixas, Tânia Raquel Santos, Jorge Machado, Luís Tavares, Fernando Bernardo, Teresa Semedo-Lemsaddek, Manuela Oliveira
The increase in prevalence of Salmonella 1,4,[5],12:i:- related infections over the last few years has been considered a public health issue in many European countries, especially as this serovar may be associated with tetraresistance to ampicillin, streptomycin, sulfonamides, and tetracyclines (R-type ASSuT). Salmonella 1,4,[5],12:i:- isolates (n = 187) obtained by the Portuguese National Laboratory from different sources, including human clinical cases (n = 170), veterinary (n = 10), environmental (n = 6), and food samples (n = 1), were collected from 15 districts between 2006 and 2011...
October 21, 2016: Foodborne Pathogens and Disease
Jingqing Liu, Hongxing Ren, Xianbei Ye, Wei Wang, Yan Liu, Liping Lou, Dongqing Cheng, Xiaofang He, Xiaoyan Zhou, Shangde Qiu, Liusong Fu, Baolan Hu
Biofilms in the pipe wall may lead to water quality deterioration and biological instability in drinking water distribution systems (DWDSs). In this study, bacterial community radial-spatial distribution in biofilms along the pipe wall in a chlorinated DWDS of East China was investigated. Three pipes of large diameter (300, 600, and 600 mm) were sampled in this DWDS, including a ductile cast iron pipe (DCIP) with pipe age of 11 years and two gray cast iron pipes (GCIP) with pipe ages of 17 and 19 years, and biofilms in the upper, middle, and lower parts of each pipe wall were collected...
October 19, 2016: Applied Microbiology and Biotechnology
Irfan Ahmad, Syed Fazle Rouf, Lei Sun, Annika Cimdins, Sulman Shafeeq, Soazig Le Guyon, Marco Schottkowski, Mikael Rhen, Ute Römling
BACKGROUND: Cellulose, a 1,4 beta-glucan polysaccharide, is produced by a variety of organisms including bacteria. Although the production of cellulose has a high biological, ecological and economical impact, regulatory mechanisms of cellulose biosynthesis are mostly unknown. Family eight cellulases are regularly associated with cellulose biosynthesis operons in bacteria; however, their function is poorly characterized. In this study, we analysed the role of the cellulase BcsZ encoded by the bcsABZC cellulose biosynthesis operon of Salmonella enterica serovar Typhimurium (S...
October 19, 2016: Microbial Cell Factories
Serena Ammendola, Ylenia D'Amico, Barbara Chirullo, Rosanna Drumo, Domenico Civardelli, Paolo Pasquali, Andrea Battistoni
Zinc is known to play a central role in bacterial physiology and pathogenesis. Here, we report that the accumulation of FliC, the structural subunit of Salmonella phase 1 flagella, is sharply reduced in a znuABC Salmonella enterica sv. Typhimurium strain grown in zinc-poor media. Consequently, this mutant strain lacks motility, unless it grows in zinc-replete environments. This phenotype is the consequence of a general downregulation of all the genes involved in the biosynthesis of flagella, suggesting that zinc is the cofactor of proteins involved in the initiation of the transcriptional regulatory cascade leading to flagella assembly...
October 1, 2016: Metallomics: Integrated Biometal Science
Serena Ammendola, Ylenia D'Amico, Barbara Chirullo, Rosanna Drumo, Domenico Ciavardelli, Paolo Pasquali, Andrea Battistoni
Correction for 'Zinc is required to ensure the expression of flagella and the ability to form biofilms in Salmonella enterica sv Typhimurium' by Serena Ammendola et al., Metallomics, 2016, DOI: 10.1039/c6mt00108d.
October 5, 2016: Metallomics: Integrated Biometal Science
Ahmed AbdelKhalek, Charles R Ashby, Bhargav A Patel, Tanaji T Talele, Mohamed N Seleem
Bacterial infections present a serious challenge to healthcare practitioners due to the emergence of resistance to numerous conventional antibacterial drugs. Therefore, new bacterial targets and new antimicrobials are unmet medical needs. Rhodanine derivatives have been shown to possess potent antimicrobial activity via a novel mechanism. However, their potential use as antibacterials has not been fully examined. In this study, we determined the spectrum of activity of seven rhodanine derivatives (compounds Rh 1-7) against clinical isolates of Gram-positive and Gram-negative bacterial strains and Candida albicans...
2016: PloS One
Stefania Carpino, Cinzia L Randazzo, Alessandra Pino, Nunziatina Russo, Teresa Rapisarda, Gianni Belvedere, Cinzia Caggia
The objectives of the present study were to characterize the biofilm microbiota of 11 different farms (from A to K), producing PDO Ragusano cheese, and to investigate on its ability to generate volatile organic compounds (VOCs) in milk samples inoculated with biofilm and incubated under Ragusano cheese making conditions. The biofilms were subjected to plate counting and PCR/T/DGGE analysis and the VOCs generated in incubated milk samples were evaluated through SmartNose, GC/O, and GC/MS. Streptococcus thermophilus was the dominant species both in biofilms and in incubated milks...
February 2017: Food Microbiology
Inna Shomer, Alon Avisar, Prerak Desai, Shalhevet Azriel, Gill Smollan, Natasha Belausov, Nathan Keller, Daniel Glikman, Yasmin Maor, Avi Peretz, Michael McClelland, Galia Rahav, Ohad Gal-Mor
Salmonella enterica serovar Enteritidis (S. Enteritidis) is one of the ubiquitous Salmonella serovars worldwide and a major cause of food-born outbreaks, which are often associated with poultry and poultry derivatives. Here we report a nation-wide S. Enteritidis clonal outbreak that occurred in Israel during the last third of 2015. Pulsed field gel electrophoresis and whole genome sequencing identified genetically related strains that were circulating in Israel as early as 2008. Global comparison linked this outbreak strain to several clinical and marine environmental isolates that were previously isolated in California and Canada, indicating that similar strains are prevalent outside of Israel...
2016: Frontiers in Microbiology
Jinsong Feng, Guillaume Lamour, Rui Xue, Mehr Negar Mirvakliki, Savvas G Hatzikiriakos, Jie Xu, Hongbin Li, Shuo Wang, Xiaonan Lu
Campylobacter jejuni is a microaerophilic pathogen and leading cause of human gastroenteritis. The presence of C. jejuni encased in biofilms found in meat and poultry processing facilities may be the major strategy for its survival and dissemination in aerobic environment. In this study, Staphylococcus aureus, Salmonella enterica, or Pseudomonas aeruginosa was mixed with C. jejuni F38011 as a culture to form dual-species biofilms. After 4days' exposure to aerobic stress, no viable C. jejuni cells could be detected from mono-species C...
December 5, 2016: International Journal of Food Microbiology
Yogesh D Walawalkar, Yatindra Vaidya, Vijayashree Nayak
Salmonella Typhi can chronically persist within the gallbladder of patients suffering from gallbladder diseases. This study, intended to improve our understanding of bacterial mechanisms underlying bile adaptation, revealed that bile, which is a bactericidal agent, led to the generation of reactive oxygen species in S Typhi. Salmonella Typhi in response showed a significant increase in the production of anti-oxidative enzymes, namely superoxide dismutase and catalase. The work reports that the quorum-sensing (QS) system of S Typhi regulates the level of these enzymes during oxidative stress...
November 2016: Pathogens and Disease
Haley E Adcox, Erin M Vasicek, Varun Dwivedi, Ky V Hoang, Joanne Turner, John S Gunn
Salmonella enterica serovar Typhi, the causative agent of typhoid fever in humans, form biofilms encapsulated by an extracellular matrix (ECM). Biofilms facilitate colonization and persistent infection in gallbladders of humans and mouse models of chronic carriage. Individual roles of matrix components have not been completely elucidated in vitro or in vivo To examine individual functions, strains of S. Typhimurium, the murine model of S Typhi, in which various ECM genes were deleted or added were created to examine biofilm formation and colonization and persistence in the gallbladder...
September 6, 2016: Infection and Immunity
A Lamas, J M Miranda, B Vázquez, A Cepeda, C M Franco
Salmonella enterica subsp. enterica is one of the main food-borne pathogens. This microorganism combines an aerobic life outside the host with an anaerobic life within the host. One of the main concerns related to S. enterica is biofilm formation and cellulose production. In this study, biofilm formation, morphotype, cellulose production and transcription of biofilm and quorum sensing-related genes of 11 S. enterica strains were tested under three different conditions: aerobiosis, microaerobiosis, and anaerobiosis...
December 5, 2016: International Journal of Food Microbiology
Courtney Reichhardt, Oscar A McCrate, Xiaoxue Zhou, Jessica Lee, Wiriya Thongsomboon, Lynette Cegelski
Microbial biofilms are communities of cells characterized by a hallmark extracellular matrix (ECM) that confers functional attributes to the community, including enhanced cohesion, adherence to surfaces, and resistance to external stresses. Understanding the composition and properties of the biofilm ECM is crucial to understanding how it functions and protects cells. New methods to isolate and characterize ECM are emerging for different biofilm systems. Solid-state nuclear magnetic resonance was used to quantitatively track the isolation of the insoluble ECM from the uropathogenic Escherichia coli strain UTI89 and understand the role of Congo red in purification protocols...
November 2016: Analytical and Bioanalytical Chemistry
Serajus Salaheen, Ekta Jaiswal, Jungsoo Joo, Mengfei Peng, Ryan Ho, Danielle OConnor, Katrina Adlerz, Jose Helim Aranda-Espinoza, Debabrata Biswas
The aim of this study was to evaluate the phenotypic and genotypic properties of Salmonella enterica serovar Typhimurium (ST) in the presence of lethal and sublethal concentrations (SLC2LOG) of blackberry (Rubus fruticosus) and blueberry (Vaccinium corymbosum) pomace extracts. Antimicrobial susceptibility, physicochemical properties, motility, biofilm formation ability, virulence gene expression patterns, and the ability of ST to colonize in chick cecum were evaluated in the presence of these bioactive extracts...
November 21, 2016: International Journal of Food Microbiology
Elien Peeters, Geert Hooyberghs, Stijn Robijns, Kai Waldrant, Ami De Weerdt, Nicolas Delattin, Veerle Liebens, Soňa Kucharíková, Hélène Tournu, Natalie Verstraeten, Barbara Dovgan, Lenart Girandon, Mirjam Fröhlich, Katrijn De Brucker, Patrick Van Dijck, Jan Michiels, Bruno P A Cammue, Karin Thevissen, Jozef Vanderleyden, Erik Van der Eycken, Hans P Steenackers
Previously, we have synthesized several series of compounds, based on the 5-aryl-2-aminoimidazole scaffold, that show a preventive activity against biofilm formation of Salmonella Typhimurium and Pseudomonas aeruginosa Here, we further studied the activity spectrum of a number of the most active N1- and 2N-substituted compounds against a broad panel of monospecies and mixed species biofilms of bacteria and fungi. A N1-substituted compound showed very strong anti-biofilm activity against Gram-negatives and Gram-positives and against the fungus Candida albicans, but was previously shown to be toxic against various eukaryotic cell lines...
August 22, 2016: Antimicrobial Agents and Chemotherapy
Wu Shuai-Cheng, Fu Ben-Dong, Chu Xiu-Ling, Su Jian-Qing, Fu Yun-Xing, Cui Zhen-Qiang, Xu Dao-Xiu, Wu Zong-Mei
Phloretin, a natural component of many fruits, exhibits anti-virulence effects and provides a new alternative to counter bacterial infection. The aim of this study was to determine the effect of subinhibitory concentrations of phloretin on the virulence of Salmonella typhimurium. At concentrations where growth of Salmonella was not inhibited, phloretin significantly inhibited bacteria biofilm formation and motility. Subinhibitory concentrations of phloretin repressed eight genes involved in the Salmonella pathogenicity island 1 and 3 genes involved in flagella production...
November 2016: Antonie Van Leeuwenhoek
Mariya I Petrova, Nicole C E Imholz, Tine L A Verhoeven, Jan Balzarini, Els J M Van Damme, Dominique Schols, Jos Vanderleyden, Sarah Lebeer
OBJECTIVES: Increased antibiotic resistance has catalyzed the research on new antibacterial molecules and alternative strategies, such as the application of beneficial bacteria. Since lectin molecules have unique sugar-recognizing capacities, and pathogens are often decorated with sugars that affect their survival and infectivity, we explored whether lectins from the probiotic strain Lactobacillus rhamnosus GG have antipathogenic properties. METHODS: The genome sequence of L...
2016: PloS One
Cristina Latasa, Maite Echeverz, Begoña García, Carmen Gil, Enrique García-Ona, Saioa Burgui, Noelia Casares, Sandra Hervás-Stubbs, Juan José Lasarte, Iñigo Lasa, Cristina Solano
Salmonellosis is one of the most important bacterial zoonotic diseases transmitted through the consumption of contaminated food, with chicken and pig related products being key reservoirs of infection. Although numerous studies on animal vaccination have been performed in order to reduce Salmonella prevalence, there is still a need for an ideal vaccine. Here, with the aim of constructing a novel live attenuated Salmonella vaccine candidate, we firstly analyzed the impact of the absence of cyclic-di-GMP (c-di-GMP) in Salmonella virulence...
2016: PloS One
Larissa Balabanova, Olga Nedashkovskaya, Anna Podvolotskaya, Lubov Slepchenko, Vasily Golotin, Alexey Belik, Ludmila Shevchenko, Oksana Son, Valery Rasskazov
Data is presented in support of functionality of hyper-diverse protein families encoded by the Cobetia amphilecti KMM 296 (formerly Cobetia marina KMM 296) genome ("The genome of the marine bacterium Cobetia marina KMM 296 isolated from the mussel Crenomytilus grayanus (Dunker, 1853)" [1]) providing its nutritional versatility, adaptability and biocontrol that could be the basis of the marine bacterium evolutionary and application potential. Presented data include the information of growth and biofilm-forming properties of the food-associated isolates of Pseudomonas, Bacillus, Listeria, Salmonella and Staphylococcus under the conditions of their co-culturing with C...
September 2016: Data in Brief
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