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https://www.readbyqxmd.com/read/29128733/prevalence-of-curli-genes-among-cronobacter-species-and-their-roles-in-biofilm-formation-and-cell-cell-aggregation
#1
Lan Hu
Cronobacter species are food-borne opportunistic pathogens that cause sepsis, meningitis, and necrotizing enterocolitis in neonates. Bacterial pathogens such as pathogenic Escherichia coli and Salmonella species express extracellular curli fimbriae that are involved in rugosity, biofilm formation, and host cell adherence. csgBAC operon encodes the major curli structural subunit CsgA and the nucleator protein CsgB. csgDEFG operon encodes the regulatory protein CsgD and putative assembly factors. In this study, the curli operons were analyzed in the sequences of 13 Cronobacter strains and other enteric bacterial pathogens...
October 31, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29125121/structural-basis-of-host-recognition-and-biofilm-formation-by-salmonella-saf-pili
#2
Longhui Zeng, Li Zhang, Pengran Wang, Guoyu Meng
Pili are critical in host recognition, colonization and biofilm formation during bacterial infection. Here, we report the crystal structures of SafD-dsc and SafD-SafA-SafA (SafDAA-dsc) in Saf pili. Cell adherence assays show that SafD and SafA are both required for host recognition, suggesting a poly-adhesive mechanism for Saf pili. Moreover, the SafDAA-dsc structure, as well as SAXS characterization, reveals an unexpected inter-molecular oligomerization, prompting the investigation of Saf-driven self-association in biofilm formation...
November 10, 2017: ELife
https://www.readbyqxmd.com/read/29112725/peptide-transporter-yjiy-influences-the-expression-of-the-virulence-gene-mgtc-to-regulate-biofilm-formation-in-salmonella
#3
Preeti Garai, Kasturi Chandra, Jayanta Chatterjee, Dipshikha Chakravortty
Biofilm is one of the coping strategies of Salmonella against antimicrobial environmental stresses including nutrient starvation. However, channeling of the cue of starvation towards biofilm formation is not understood well. Our study shows that a carbon starvation gene, yjiY, coding for a peptide transporter, influences the expression of a virulence associated gene, mgtC, in Salmonella, to regulate biofilm formation. We demonstrate here that the mutant strain, ΔyjiY, is unable to form biofilm due to the increased expression of mgtC...
November 3, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29106298/phenotypic-and-molecular-characterization-of-salmonella-enteritidis-se86-isolated-from-poultry-and-salmonellosis-outbreaks
#4
Karen Apellanis Borges, Thales Quedi Furian, Sara Neves de Souza, Rafaela Menezes, Carlos Tadeu Pippi Salle, Hamilton Luiz de Souza Moraes, Eduardo César Tondo, Vladimir Pinheiro do Nascimento
Salmonella Enteritidis remains a standout among the leading causes of foodborne diseases worldwide. Previous studies have demonstrated that a unique clonal group of Salmonella Enteritidis, named SE86, is involved in foodborne outbreaks in southern Brazil and is frequently identified among strains isolated from poultry. The aim of this study was to determine the influence of the isolation source (food products involved in salmonellosis outbreaks and poultry sources) on the phenotypic and molecular characteristics of Salmonella Enteritidis SE86...
November 6, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/29100930/biofilm-formation-by-staphylococcus-aureus-and-salmonella-spp-under-mono-and-dual-species-conditions-and-their-sensitivity-to-cetrimonium-bromide-peracetic-acid-and-sodium-hypochlorite
#5
Maricarmen Iñiguez-Moreno, Melesio Gutiérrez-Lomelí, Pedro Javier Guerrero-Medina, María Guadalupe Avila-Novoa
The aim of this study was evaluated the biofilm formation by Staphylococcus aureus 4E and Salmonella spp. under mono and dual-species biofilms, onto stainless steel 316 (SS) and polypropylene B (PP), and their sensitivity to cetrimonium bromide, peracetic acid and sodium hypochlorite. The biofilms were developed by immersion of the surfaces in TSB by 10 d at 37°C. The results showed that in monospecies biofilms the type of surface not affected the cellular density (p>0.05). However, in dual-species biofilms on PP the adhesion of Salmonella spp...
October 13, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29096062/disassembly-of-bacterial-biofilms-by-the-self-assembled-glycolipids-derived-from-renewable-resources
#6
Yadavali Siva Prasad, Sandeep Miryala, Krishnamoorthy Lalitha, K Ranjitha, Shehnaz Barbhaiwala, Vellaisamy Sridharan, C Uma Maheswari, C S Srinandan, Subbiah Nagarajan
More than 80% of chronic infections of bacteria are caused by biofilms. It is also a long-term survival strategy of the pathogens in a nonhost environment. Several amphiphilic molecules have been used in the past to potentially disrupt biofilms; however, the involvement of multistep synthesis, complicated purification and poor yield still remains a major problem. Herein, we report a facile synthesis of glycolipid based surfactant from renewable feedstocks in good yield. The nature of carbohydrate unit present in glycolipid influence the ring chain tautomerism, which resulted in the existence of either cyclic structure or both cyclic and acyclic structures...
November 10, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29093703/caprylic-and-polygalacturonic-acid-combinations-for-eradication-of-microbial-organisms-embedded-in-biofilm
#7
Joel Rosenblatt, Ruth A Reitzel, Nylev Vargas-Cruz, Anne-Marie Chaftari, Ray Hachem, Issam Raad
There is a need for non-antibiotic, antimicrobial compositions with low toxicity capable of broad-spectrum eradication of pathogenic biofilms in food preparation and healthcare settings. In this study we demonstrated complete biofilm eradication within 60 min with synergistic combinations of caprylic and polygalacturonic (PG) acids in an in vitro biofilm eradication model against representative hospital and foodborne infectious pathogen biofilms (methicillin-resistant Staphylococcus aureus, multidrug-resistant Pseudomonas aeruginosa, Candida albicans, Escherichia coli, and Salmonella enteritidis)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29089942/the-possible-influence-of-non-synonymous-point-mutations-within-the-fima-adhesin-of-non-typhoidal-salmonella-nts-isolates-in-the-process-of-host-adaptation
#8
Sahar Alshalchi, Shivdeep S Hayer, Ran An, Jeannette Munoz-Aguayo, Christian Flores-Figueroa, Ryan Nguyen, Dale Lauer, Karen Olsen, Julio Alvarez, David Boxrud, Carol Cardona, Sinisa Vidovic
Non-typhoidal Salmonella (NTS) remains a global pathogen that affects a wide range of animal species. We analyzed a large number of NTS isolates of different host origins, including Salmonella Heidelberg (n = 80, avian), S. Dublin (50, bovine), S. Typhimurium var 5- (n = 40, porcine), S. 4,5,12,:i:- (n = 40, porcine), S. Cerro (n = 16, bovine), and S. Montevideo (n = 14, bovine), using virulence profiling of the bcfC, mgtC, ssaC, invE, pefA, stn, sopB, and siiE virulence-associated genes, a biofilm production assay, pulsed field gel electrophoresis, and the full-length sequencing of the fimA (adhesin) and iroN (receptor) genes...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29089020/transcriptomic-study-of-salmonella-enterica-subspecies-enterica-serovar-typhi-biofilm
#9
Khee Chian Jason Chin, Todd Duane Taylor, Maxime Hebrard, Kogaan Anbalagan, Marjan Ganjali Dashti, Kia Kien Phua
BACKGROUND: Typhoid fever is an acute systemic infection of humans caused by Salmonella enterica subspecies enterica serovar Typhi (S. Typhi). In chronic carriers, the bacteria survive the harsh environment of the gallbladder by producing biofilm. The phenotype of S. Typhi biofilm cells is significantly different from the free-swimming planktonic cells, and studies have shown that they are associated with antibiotic resistance, immune system evasion, and bacterial persistence. However, the mechanism of this transition and the events leading to biofilm formation are unknown...
October 31, 2017: BMC Genomics
https://www.readbyqxmd.com/read/29058454/anti-bacterial-and-anti-biofilm-activity-of-commercial-organic-acid-products-against-salmonella-enterica-isolates-recovered-from-egg-farm-environment
#10
Vivek Pande, Andrea R McWhorter, Kapil K Chousalkar
This study evaluated the antibacterial activity of commercially available organic acid water additives against Salmonella enterica isolates and examined the susceptibility of Salmonella Typhimurium biofilms to these products. Three commercial organic acid products (A, B, and C) were evaluated for minimum inhibitory and bactericidal concentrations against isolates of S. enterica serovars. Three and five day old S. Typhimurium biofilms were formed at 22 ± 2°C using MBEC™ assay system and exposed for 30 and 90 min at 0...
October 23, 2017: Avian Pathology: Journal of the W.V.P.A
https://www.readbyqxmd.com/read/29053370/cross-contamination-and-biofilm-formation-by-salmonella-enterica-serovar-enteritidis-on-various-cutting-boards
#11
Stéfani T A Dantas, Bruna F Rossi, Erika C R Bonsaglia, Ivana G Castilho, Rodrigo T Hernandes, Ary Fernandes Júnior, Vera L M Rall
Cross-contamination is one of the main factors related to foodborne outbreaks. This study aimed to analyze the cross-contamination process of Salmonella enterica serovar Enteritidis from poultry to cucumbers, on various cutting board surfaces (plastic, wood, and glass) before and after washing and in the presence and absence of biofilm. Thus, 10 strains of Salmonella Enteritidis were used to test cross-contamination from poultry to the cutting boards and from thereon to cucumbers. Moreover, these strains were evaluated as to their capacity to form biofilm on hydrophobic (wood and plastic) and hydrophilic materials (glass)...
October 20, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/29035101/biofilm-formation-antimicrobial-resistance-and-sanitizer-tolerance-of-salmonella-enterica-strains-isolated-from-beef-trim
#12
Rong Wang, John W Schmidt, Dayna M Harhay, Joseph M Bosilevac, David A King, Terrance M Arthur
In the beef industry, product contamination by Salmonella enterica is a serious public health concern, which may result in human infection and cause significant financial loss due to product recalls. Currently, the precise mechanism and pathogen source responsible for Salmonella contamination in commercial establishments are not well understood. We characterized 89 S. enterica strains isolated from beef trim with respect to their biofilm-forming ability, antimicrobial resistance, and biofilm cell survival/recovery growth after sanitizer exposure...
October 16, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/29027368/expression-of-fluorescent-proteins-in-lactobacillus-rhamnosus-to-study-host-microbe-and-microbe-microbe-interactions
#13
Irina Spacova, Elke Lievens, Tine Verhoeven, Hans Steenackers, Jos Vanderleyden, Sarah Lebeer, Mariya I Petrova
Probiotic Lactobacillus strains are widely used to benefit human and animal health, although the exact mechanisms behind their interactions with the host and the microbiota are largely unknown. Fluorescent tagging of live probiotic cells is an important tool to unravel their modes of action. In this study, the implementation of different heterologously expressed fluorescent proteins for the labelling of the model probiotic strains Lactobacillus rhamnosusGG (gastrointestinal) and Lactobacillus rhamnosusGR-1 (vaginal) was explored...
October 13, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/29018430/preliminary-transcriptome-analysis-of-mature-biofilm-and-planktonic-cells-of-salmonella-enteritidis-exposure-to-acid-stress
#14
Kun Jia, Guangyu Wang, Lijiao Liang, Meng Wang, Huhu Wang, Xinglian Xu
Salmonella has emerged as a well-recognized food-borne pathogen, with many strains able to form biofilms and thus cause cross-contamination in food processing environments where acid-based disinfectants are widely encountered. In the present study, RNA sequencing was employed to establish complete transcriptome profiles of Salmonella Enteritidis in the forms of planktonic and biofilm-associated cells cultured in Tryptic Soytone Broth (TSB) and acidic TSB (aTSB). The gene expression patterns of S. Enteritidis significantly differed between biofilm-associated and planktonic cells cultivated under the same conditions...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28974445/cinnamaldehyde-induces-changes-in-the-protein-profile-of-salmonella-typhimurium-biofilm
#15
Alex Fiori Silva, Adriele Rodrigues Dos Santos, Daliah Alves Coelho Trevisan, Alessandra Braga Ribeiro, Paula Aline Zanetti Sá-Campanerut, Caroline Kukolj, Emanuel Maltempi de Souza, Rosilene Fressatti Cardoso, Terezinha Inez Estivalet Svidzinski, Benicio Alves de Abreu Filho, Miguel Machinski Junior, Jane Martha Graton Mikcha
The effect of cinnamaldehyde against biofilm cells of Salmonella Typhimurium ATCC 14028 was evaluated. We also assessed differential protein patterns that were expressed by biofilms compared with planktonic cells and protein expression by cinnamaldehyde-treated biofilms cells. This compound decreased biofilm biomass and metabolic activity of biofilms at both concentrations tested. Cinnamaldehyde treatment reduced the number of attached cells in polypropylene, reflected by colony count and scanning electron microscopy...
September 30, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28968533/using-the-agricultural-environment-to-select-better-surrogates-for-foodborne-pathogens-associated-with-fresh-produce
#16
Kimberly L Cook, Ethan C Givan, Holly M Mayton, Rohan R Parekh, Ritchie Taylor, Sharon L Walker
Despite continuing efforts to reduce foodborne pathogen contamination of fresh produce, significant outbreaks continue to occur. Identification of appropriate surrogates for foodborne pathogens facilitates relevant research to identify reservoirs and amplifiers of these contaminants in production and processing environments. Therefore, the objective of this study was to identify environmental Escherichia coli isolates from manures (poultry, swine and dairy) and surface water sources with properties similar to those of the produce associated foodborne pathogens E...
December 4, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28963222/draft-genome-sequences-of-biofilm-forming-and-non-biofilm-forming-nontyphoidal-salmonella-enterica-serovars
#17
Deeksha Shetty, Alexander A Grigoryan, Sahar Alshalchi, Niradha Withana Gamage, Julie Roy, John R Lawrence, Sinisa Vidovic, Darren R Korber
The genetic basis for biofilm formation among nontyphoidal salmonellae (NTS) remains poorly understood. This draft genome submission provides initial insights on the genetic differences between biofilm-forming and non-biofilm-forming clinical and environmental NTS serovars.
September 28, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28954956/preincubation-of-escherichia-coli-atcc-25922-with-nacl-increases-its-attachment-to-lettuce-surfaces-compared-with-other-chemicals
#18
Kazuhisa Tomita, Jun Sawai
 The inhibition of microbial attachment to food is important for the prevention of cross-contamination during food processing. The effect of several chemicals that were added in an Escherichia coli growth medium on the attachment of the bacterium to lettuce was investigated. E. coli ATCC 25922, which is reportedly a useful surrogate for E. coli O157:H7 in surface attachment studies, was preincubated in a nutrient broth (NB) containing sodium chloride, potassium chloride, sodium deoxycholate, sodium linear alkylbenzene sulfonate, or sorbic acid...
2017: Biocontrol Science
https://www.readbyqxmd.com/read/28944561/impact-of-persistent-and-nonpersistent-generic-escherichia-coli-and-salmonella-sp-recovered-from-a-beef-packing-plant-on-biofilm-formation-by-e-%C3%A2-coli-o157
#19
J Visvalingam, T C Ells, X Yang
AIMS: To examine the influence of meat plant Escherichia coli and Salmonella sp. isolates on E. coli O157 biofilm formation. METHODS AND RESULTS: Biofilm formation was quantified by crystal violet staining (A570 nm ) and viable cell numbers for up to 6 days at 15°C. All five persistent E. coli genotypes formed strong biofilms when cultured alone or co-cultured with E. coli O157, with A570 nm values reaching ≥4·8 at day 4, while only two of five nonpersistent genotypes formed such biofilms...
September 25, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28941894/development-of-non-pathogenic-bacterial-biofilms-on-the-surface-of-stainless-steel-which-are-inhibitory-to-salmonella-enterica
#20
Yoonbin Kim, Hoikyung Kim, Larry R Beuchat, Jee-Hoon Ryu
Non-pathogenic bacterial biofilms were developed on the surface of stainless steel possessing desiccation tolerance and antimicrobial activity against Salmonella enterica. Three bacteria exhibiting strong antimicrobial activities against S. enterica were isolated from various soils, foods, and food-contact surfaces. Isolates were identified as Pseudomonas extremorientalis (strain Lettuce-28), Paenibacillus peoriae (strain Lettuce-7), and Streptomyces cirratus (strain Geumsan-207). These bacteria grew rapidly and formed biofilms within 24 h on the surface of stainless steel coupons (SSCs) immersed in laboratory media (tryptic soy broth or Bennet's broth) at 25 °C...
February 2018: Food Microbiology
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