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Salmonella biofilms

Piumi De Abrew Abeysundara, Nitin Dhowlaghar, Ramakrishna Nannapaneni, Mark W Schilling, Barakat Mahmoud, Chander S Sharma, Din-Pow Ma
Salmonella enterica is responsible for the highest number of foodborne disease outbreaks pertaining to cantaloupe industry. The objective of this study was to examine the growth and biofilm formation by outbreak strains of S. enterica ser. Poona (S. Poona), S. enterica ser. Stanley (S. Stanley) and S. enterica ser. Montevideo (S. Montevideo) on different food-contact processing surfaces in cantaloupe flesh and peel extracts at 22 °C and 10 °C. The generation time of all S. enterica strains tested was shorter in the high concentration (50 mg/ml) of cantaloupe extract and high temperature...
May 4, 2018: International Journal of Food Microbiology
Felipe Alves de Almeida, Erika Lorena Giraldo Vargas, Deisy Guimarães Carneiro, Uelinton Manoel Pinto, Maria Cristina Dantas Vanetti
Salmonella belongs to the Enterobacteriaceae family which is widely distributed in the environment due to its adaptive capacity to stress conditions. In addition, Salmonella is able to perform a type of cell-to-cell communication called quorum sensing, which leads to differential gene expression. The quorum sensing system mediated by AI-1, acyl homoserine lactones (AHLs), is incomplete in Salmonella because the luxI homolog gene, which encodes for AI-1 synthase, is missing in the genome. However, a homologue of LuxR, known as SdiA, is present and allows the detection of signaling molecules produced by other species of bacteria, leading to regulation of gene expression, mainly related to virulence and biofilm formation...
May 12, 2018: Microbial Pathogenesis
Xin Zhao, Rui Liu, Hao Tang, George Osei-Adjei, Shungao Xu, Ying Zhang, Xinxiang Huang
Bacterial non-coding RNAs (ncRNAs) are widely studied and found to play important roles in regulating various cellular processes. Recently, many ncRNAs have been discovered to be transcribed or processed from 3' untranslated regions (3' UTRs). Here we reported a novel 3' UTR-derived ncRNA, RibS, which could influence biofilm formation of Salmonella enterica serovar Typhi (S. Typhi). RibS was confirmed to be a ∼700 nt processed product produced by RNase III-catalyzed cleavage from the 3' UTR of riboflavin synthase subunit alpha mRNA, RibE...
May 8, 2018: Research in Microbiology
Robert Lotha, Niranjana SriSundaramoorthy, Bhanuvalli R Shamprasad, Saisubramanian Nagarajan, Aravind Sivasubramanian
Purified plant nutraceuticals afzelin and quercetrin from an edible plant- Crotolaria tetragona was employed for the fabrication of silver nanoparticles (AgNPs) by a sunlight mediated process. From among a panel of strains tested, AgNPs displayed potent bacteriostatic and bactericidal effect against P. aeruginosa and S. typhimurium. Time kill studies revealed green synthesized AgNPs displayed comparable bactericidal effect with chemically synthesized AgNPs against S. typhimurium. Antibiofilm potential of AgNPs showed that they were highly effective at sub MIC concentrations in causing 50% biofilm inhibition against food borne pathogen S...
April 28, 2018: Microbial Pathogenesis
Nitin Dhowlaghar, Mohit Bansal, Mark W Schilling, Ramakrishna Nannapaneni
The objective of this study was to determine the growth and survival of Salmonella enterica in the presence of high and low concentrations (375 μg/ml and 15 μg/ml) of catfish mucus extract at 10 °C and 22 °C for 63 days. The second objective of this study was to investigate the biofilm formation of Salmonella enterica serovar Blockley (7175) in catfish mucus extract for 48 h at 22 °C on four food-contact surfaces and to observe the biofilm populations using Scanning Electron Microscopy (SEM)...
September 2018: Food Microbiology
Agnieszka Chlebicz, Katarzyna Śliżewska
Zoonoses are diseases transmitted from animals to humans, posing a great threat to the health and life of people all over the world. According to WHO estimations, 600 million cases of diseases caused by contaminated food were noted in 2010, including almost 350 million caused by pathogenic bacteria. Campylobacter , Salmonella , as well as Yersinia enterocolitica and Listeria monocytogenes may dwell in livestock (poultry, cattle, and swine) but are also found in wild animals, pets, fish, and rodents. Animals, often being asymptomatic carriers of pathogens, excrete them with faeces, thus delivering them to the environment...
April 26, 2018: International Journal of Environmental Research and Public Health
Lulu Liu, Yi Xu, Feiyun Cui, Ye Xia, Li Chen, Xiaojing Mou, Junjiang Lv
A biosensor chip integrated interdigital microelectrodes was proposed and applied to monitor the formation process of Salmonella and E. coli biofilms in this paper. The biosensor chip was composed of a glass substrate with interdigital microelectrodes and PDMS layer with micro cavities. The electrochemical impedance spectroscopy (EIS) of Salmonella and E. coli biofilms was measured by the biosensor chip using alternating voltage of 100 mv in the frequency range from 1 Hz to 100 kHz for 48 h. It was illustrated that the changes of impedance spectroscopy of biofilms occurred with culture time...
April 12, 2018: Biosensors & Bioelectronics
Zhaohao Shi, Pravin R Kaldhone, Bijay K Khajanchi, Steven L Foley, Steven C Ricke
The draft genome sequences of four Salmonella enterica serovar Enteritidis and Kentucky isolates were evaluated for biofilm formation and antibiotic resistance. The Salmonella serovar Kentucky strains CFS84 and CFS85 and Salmonella serovar Enteritidis strains CFS86 and CFS87 were isolated from retail poultry sources in Arkansas.
April 5, 2018: Genome Announcements
Phumudzo Budeli, Resoketswe Charlotte Moropeng, Lizzy Mpenyana-Monyatsi, Maggie Ndombo Benteke Momba
Development of biofilms occurring on the inner surface of storage vessels offers a suitable medium for the growth of microorganisms and consequently contributes to the deterioration of treated drinking water quality in homes. The aim of this study was to determine whether the two point-of-use technologies (biosand zeolite silver-impregnated clay granular (BSZ-SICG) filter and silver-impregnated porous pot (SIPP) filter) deployed in a rural community of South Africa could inhibit the formation of biofilm on the surface of plastic-based containers generally used by rural households for the storage of their drinking water...
2018: PloS One
Wanbing Liu, Yafang Tan, Shiyang Cao, Haihong Zhao, Haihong Fang, Xiaoyan Yang, Tong Wang, Yazhou Zhou, Yanfeng Yan, Yanping Han, Yajun Song, Yujing Bi, Xiaoyi Wang, Ruifu Yang, Zongmin Du
Recent studies have revealed that acetylation is a widely used protein modification in prokaryotic organisms. The major protein acetylation acetyltransferase YfiQ and the sirtuin-like deacetylase CobB have been found to be involved in basic physiological processes such as primary metabolism, chemotaxis and stress responses in Escherichia coli and Salmonella However, little is known about protein acetylation modifications in Yersinia pestis, a lethal pathogen responsible for millions of human deaths in three worldwide pandemics...
April 2, 2018: Infection and Immunity
Seong B Park, Christy S Steadman, Atul A Chaudhari, Shreekumar R Pillai, Shree R Singh, Peter L Ryan, Scott T Willard, Jean M Feugang
BACKGROUND: Synthesis of silver nano-compounds with enhanced antimicrobial effects is of great interest for the development of new antibacterial agents. Previous studies have reported the antibacterial properties of pegylated silver-coated carbon nanotubes (pSWCNT-Ag) showing less toxicity in human cell lines. However, the mechanism underlining the pSWCNT-Ag as a bactericidal agent remained unfolded. Here we assessed the pSWCNT-Ag effects against foodborne pathogenic bacteria growth and proteome profile changes...
March 27, 2018: Journal of Nanobiotechnology
Soo-Jin Jung, Shin Young Park, Sang-Do Ha
The present study investigated the synergistic bactericidal effects of combined X-ray irradiation (0.5, 1.0, 1.5, and 2.0 kGy) and sodium hypochlorite (NaOCl) (50, 100, 150, 200, and 300 ppm) treatment on the reduction of S. enterica serovar Typhimurium ATCC 14028 biofilms on quail eggshells. Additionally, the color change of the quail eggshells was measured by hunter color "L" (lightness), "a" (red/green), "b" (yellow/blue), and "ΔE" (total color difference). Additionally, the puncture force was tested to evaluate eggshell thickness after the combined treatments...
May 2018: Food Research International
Xiangyu Ju, Junjie Li, Mengjiao Zhu, Zhaoxin Lu, Fengxia Lv, Xiaoyu Zhu, Xiaomei Bie
Biofilms are communities of bacterial cells that serve to protect them from external adverse influences and enhance bacterial resistance to antibiotics and sanitizers. Here, we studied the regulatory effects of glucose and sodium chloride on biofilm formation in Salmonella serovar Dublin (S. Dublin). To analyze expression levels of the quorum sensing gene luxS, we created a luxS knockout mutant. Also, antimicrobial resistance, hydrophobicity and autoinducer-2 (AI-2) activity of both the wild-type (WT) and the mutant strain were investigated...
May 2018: Food Research International
Ioannis Iliadis, Aikaterini Daskalopoulou, Manuel Simões, Efstathios Giaouris
Salmonella enterica is a major foodborne bacterial pathogen. This forms biofilms on surfaces and persists, depending on the strain and the environment. The integrative interaction of temperature (T; 13-39 °C), pH (5-8) and sodium chloride (NaCl) concentration (0.5-8.5%) on biofilm formation by two S. enterica strains (ser. Enteritidis and Typhimurium) was here evaluated under low nutrient conditions. This was achieved using response surface methodology to model the combined effect of each factor on the response, through mathematical quadratic fitting of the outcomes of a sequence of designed experiments...
May 2018: Food Research International
Karen Apellanis Borges, Thales Quedi Furian, Sara Neves de Souza, Rafaela Menezes, Diane Alves de Lima, Flávia Bornancini Borges Fortes, Carlos Tadeu Pippi Salle, Hamilton Luiz Souza Moraes, Vladimir Pinheiro Nascimento
Salmonella Enteritidis and Salmonella Typhimurium are among the most prevalent serotypes isolated from salmonellosis outbreaks and poultry. Salmonella spp. have the capacity to form biofilms on several surfaces, which can favour survival in hostile environments, such as slaughterhouses. Salmonella strains present differences in pathogenicity. However, there is little information regarding the pathogenicity of S. Enteritidis and S. Typhimurium isolated from avian sources and their relationship to biofilm production...
March 22, 2018: Microbial Pathogenesis
Michal M Dykas, Stuti K Desai, Abhijeet Patra, Mallikarjuna Rao Motapothula, Kingshuk Poddar, Linda J Kenney, Thirumalai Venkatesan
With the rise in nosocomial infections worldwide, research on materials with an intrinsic ability to inhibit biofilm formation has been generating a great deal of interest. In the present work, we describe how thin film material libraries generated by pulsed laser deposition can be used for simultaneously screening several novel metal oxide mixtures that inhibit biofilm formation in a common human pathogen, Salmonella enterica serovar Typhimurium. We discovered that in a material library constructed using two metal oxides, the net effect on biofilm formation can be modelled as an addition of the activities of the individual oxides weighted to their relative composition at that particular point on the library...
March 19, 2018: ACS Applied Materials & Interfaces
Mohammad Sadekuzzaman, Md Furkanur Rahaman Mizan, Sungdae Yang, Hyung-Suk Kim, Sang-Do Ha
Microbial biofilms pose a serious threat to food industry, as they are difficult to inactivate or remove owing to their inherent resistance to traditional physical and antimicrobial treatments. Bacteriophages have been suggested as promising biocontrol agents for eliminating biofilms within the food industry. The efficacy of phages (BP 1369 and BP 1370) was evaluated against Salmonella spp. in biofilms. Biofilms were grown on food (lettuce), food contact surfaces (stainless steel and rubber), and MBEC biofilm devices...
January 1, 2018: Food Science and Technology International, Ciencia y Tecnología de Los Alimentos Internacional
José Andrés Medrano-Félix, Cristóbal Chaidez, Kristina D Mena, María Del Socorro Soto-Galindo, Nohelia Castro-Del Campo
Survival of bacterial pathogens in different environments is due, in part, to their ability to form biofilms. Four wild-type Salmonella enterica strains, two Oranienburg and two Saintpaul isolated from river water and animal feces, were tested for biofilm formation at the air-liquid interface under stressful conditions (pH and salinity treatments such as pH 3, NaCl 4.5 w/v; pH 7, NaCl 4.5 w/v; pH 10, NaCl 4.5 w/v; pH 3, Nacl 0.5 w/v; pH 7, NaCl 0.5 w/v; and pH 10, NaCl 0.5 w/v); Salmonella Typhimurium DT104 was used as a control strain...
March 15, 2018: Environmental Monitoring and Assessment
P Verma, V V Saharan, S Nimesh, A P Singh
AIMS: The present study was designed to assess the phenotypic traits and virulence determinants of vegetable-/fruit-origin Escherichia coli and Salmonella strains. METHODS AND RESULTS: A total of 520 fresh vegetables/fruits samples were analysed for the presence of E. coli, including Shiga toxin-producing E. coli (STEC), and Salmonella. The vegetable-/fruit-origin E. coli and Salmonella strains were further assessed for antimicrobial resistance, biofilm formation, extracellular matrix production and in vitro invasion/intracellular survivability assays...
March 7, 2018: Journal of Applied Microbiology
Ilyas Alav, J Mark Sutton, Khondaker Miraz Rahman
Efflux pumps are widely implicated in antibiotic resistance because they can extrude the majority of clinically relevant antibiotics from within cells to the extracellular environment. However, there is increasing evidence from many studies to suggest that the pumps also play a role in biofilm formation. These studies have involved investigating the effects of efflux pump gene mutagenesis and efflux pump inhibitors on biofilm formation, and measuring the levels of efflux pump gene expression in biofilms. In particular, several key pathogenic species associated with increasing multidrug resistance, such as Acinetobacter baumannii, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus, have been investigated, whilst other studies have focused on Salmonella enterica serovar Typhimurium as a model organism and problematic pathogen...
February 28, 2018: Journal of Antimicrobial Chemotherapy
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