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Legionnaires disease

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https://www.readbyqxmd.com/read/28441664/protection-against-legionnaire-s-disease-recombinant-flagellin-a-of-legionella-pneumophila-can-induce-protective-immunity-against-bacteremia-in-a-balb-c-murine-model
#1
Ashraf Mohabati Mobarez, Roya Ahmadrajabi, Nima Khoramabadi, Ali-Hatef Salmanian
To investigate the immunoprotective effects of the recombinant type A flagellin (FLA), the flaA gene of Legionella pneumophila serogroup 1 strain Paris was cloned into pET28a(+). Recombinant FLA (rFLA) was overexpressed in E. coli BL21 (DE3) and purified by Ni2+ exchange chromatography. Female BALB/c aged 6-8 weeks were immunized with 20 μg of rFLA. Nonimmunized mice along with mice inoculated with a sublethal dose of live L. pneumophila intravenously were considered as negative and positive controls, respectively...
April 26, 2017: Journal of Molecular Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28403769/legionnaires-disease-cluster-investigation-in-sydney
#2
Marianne Dowsett, Emma Quinn, Leena Gupta
No abstract text is available yet for this article.
April 17, 2017: Medical Journal of Australia
https://www.readbyqxmd.com/read/28396241/molecular-typing-of-legionella-pneumophila-from-air-conditioning-cooling-waters-using-mip-gene-sbt-and-faflp-methods
#3
Xiangli Gong, Juntao Li, Ying Zhang, Shuiping Hou, Pinghua Qu, Zhicong Yang, Shouyi Chen
Legionella spp. are important waterborne pathogens. Molecular typing has become an important method for outbreaks investigations and source tracking of Legionnaires. In a survey program conducted by the Guangzhou Center for Disease Control and Prevention, multiple serotypes Legionella pneumophila (L. pneumophila) were isolated from waters in air-conditioning cooling towers in urban Guangzhou region, China between 2008 and 2011. Three genotyping methods, mip (macrophage infectivity potentiator) genotyping, SBT (sequence-based typing), and FAFLP (fluorescent amplified fragment length polymorphism analysis) were used to type these waterborne L...
April 7, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28389563/structure-of-the-wipa-protein-reveals-a-novel-tyrosine-protein-phosphatase-effector-from-legionella-pneumophila
#4
Nikos Pinotsis, Gabriel Waksman
Legionnaires disease is a severe form of pneumonia caused by the bacterium Legionella pneumophila. L. pneumophila pathogenicity relies on secretion of more than 300 effector proteins by a type IVb secretion system. Among these Legionella effectors, WipA has been primarily studied because of its dependence on a chaperone complex, IcmSW, for translocation through the secretion system, but its role in pathogenicity has remained unknown. In this study, we present the crystal structure of a large fragment of WipA, WipA435...
April 7, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28384349/neutrophils-and-ly6chi-monocytes-collaborate-in-generating-an-optimal-cytokine-response-that-protects-against-pulmonary-legionella-pneumophila-infection
#5
Cierra N Casson, Jessica L Doerner, Alan M Copenhaver, Jasmine Ramirez, Alicia M Holmgren, Mark A Boyer, Ingharan J Siddarthan, Sara H Rouhanifard, Arjun Raj, Sunny Shin
Early responses mounted by both tissue-resident and recruited innate immune cells are essential for host defense against bacterial pathogens. In particular, both neutrophils and Ly6Chi monocytes are rapidly recruited to sites of infection. While neutrophils and monocytes produce bactericidal molecules, such as reactive nitrogen and oxygen species, both cell types are also capable of synthesizing overlapping sets of cytokines important for host defense. Whether neutrophils and monocytes perform redundant or non-redundant functions in the generation of anti-microbial cytokine responses remains elusive...
April 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28378991/assessment-of-antibiotic-susceptibility-of-legionella-pneumophila-isolated-from-water-systems-in-poland
#6
Agnieszka Sikora, Iwona Gładysz, Maria Kozioł-Montewka, Małgorzata Wójtowicz-Bobin, Tomasz Stańczak, Renata Matuszewska, Bożena Krogulska
INTRODUCTION AND OBJECTIVE: Several studies have reported therapy failures in patients with legionnaires'disease; however, antimicrobial resistance of clinical and environmental isolates of Legionella spp. has not yet been documented. Routine susceptibility testing of Legionella spp. is not recommended because of difficulties in determining standard minimal inhibitory concentration values. The purpose of this study was to analyze the antimicrobial susceptibility of Legionella pneumophila...
March 21, 2017: Annals of Agricultural and Environmental Medicine: AAEM
https://www.readbyqxmd.com/read/28366526/recombinant-mip-pile-flaa-dominant-epitopes-vaccine-candidate-against-legionella-pneumophila
#7
Jinlei He, Fan Huang, Han Chen, Qiwei Chen, Junrong Zhang, Jiao Li, Dali Chen, Jianping Chen
Legionella pneumophila is the main causative agent of Legionnaires' disease, which is a severe multi-system disease with pneumonia as the primary manifestation. We designed a recombinant Mip-PilE-FlaA dominant epitopes vaccine against Legionella pneumophila to prevent the disease and evaluated its immunogenicity and protective immunity. The protein structures of Mip, PilE and FlaA were analyzed using a computer, and the gene sequences of the dominant epitopes of the three proteins were selected to construct and optimize the vaccine...
March 31, 2017: Immunology Letters
https://www.readbyqxmd.com/read/28353109/exploring-the-legionella-pneumophila-positivity-rate-in-hotel-water-samples-from-antalya-turkey
#8
Nevgün Sepin Özen, Şenay Tuğlu Ataman, Mestan Emek
The genus Legionella is a fastidious Gram-negative bacteria widely distributed in natural waters and man made water supply systems. Legionella pneumophila is the aetiological agent of approximately 90% of reported Legionellosis cases, and serogroup 1 is the most frequent cause of infections. Legionnaires' disease is often associated with travel and continues to be a public health concern at present. The correct water management quality practices and rapid methods for analyzing Legionella species in environmental water is a key point for the prevention of Legionnaires' disease outbreaks...
March 29, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28328866/the-2015-new-york-city-legionnaires-disease-outbreak-a-case-study-on-a-history-making-outbreak
#9
Allison T Chamberlain, Jonathan D Lehnert, Ruth L Berkelman
No abstract text is available yet for this article.
March 21, 2017: Journal of Public Health Management and Practice: JPHMP
https://www.readbyqxmd.com/read/28326293/pily1-promotes-legionella-pneumophila-infection-of-human-lung-tissue-explants-and-contributes-to-bacterial-adhesion-host-cell-invasion-and-twitching-motility
#10
Julia Hoppe, Can M Ünal, Stefanie Thiem, Louisa Grimpe, Torsten Goldmann, Nikolaus Gaßler, Matthias Richter, Olga Shevchuk, Michael Steinert
Legionnaires' disease is an acute fibrinopurulent pneumonia. During infection Legionella pneumophila adheres to the alveolar lining and replicates intracellularly within recruited macrophages. Here we provide a sequence and domain composition analysis of the L. pneumophila PilY1 protein, which has a high homology to PilY1 of Pseudomonas aeruginosa. PilY1 proteins of both pathogens contain a von Willebrand factor A (vWFa) and a C-terminal PilY domain. Using cellular fractionation, we assigned the L. pneumophila PilY1 as an outer membrane protein that is only expressed during the transmissive stationary growth phase...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28320877/functional-analysis-of-the-alternative-sigma-28-factor-flia-and-its-anti-sigma-factor-flgm-of-the-non-flagellated-legionella-species-l-oakridgensis
#11
Hana Tlapák, Kerstin Rydzewski, Tino Schulz, Dennis Weschka, Eva Schunder, Klaus Heuner
Legionella oakridgensis is causing the Legionnaires' disease but is known to be less virulent than Legionella pneumophilaL. oakridgensis is one of the Legionella species that is non-flagellated. The genes of the flagellar regulon are absent; except the alternative sigma-28 factor (FliA) and its anti-sigma-28 factor (FlgM). Similar to L. oakridgensis, Legionella adelaidensis and Legionella londiniensis, located in the same phylogenetic clade, they have no flagellar regulon, although both are positive for fliA and flgM Herein, we investigated the role and function of both genes to better understand the role of FliA, the positive regulator of flagellin expression, in non-flagellated strains...
March 20, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28286615/detection-of-legionella-pneumophila-in-urine-and-serum-specimens-of-neutropenic-febrile-patients-with-haematological-malignancies
#12
Nastaran Farzi, Zahra Abrehdari-Tafreshi, Omid Zarei, Leili Chamani-Tabriz
Background: Legionella pneumophila (L. pneumophila) is a gram-negative bacterium which causes ‎Legionnaires' disease as well as Pontiac fever. The Legionella infections in patients suffering from ‎neutropenia- as a common complication of cancer chemotherapy- can distribute rapidly. We ‎aimed to detect of L. pneumophila in haematological malignancy suffering patients with ‎neutropenic fever by targeting the (macrophage infectivity potentiator) mip gene. Subjects andMethods: Serum and ‎urine specimens were obtained from 80 patients and presence of mip gene of L...
January 1, 2017: International Journal of Hematology-oncology and Stem Cell Research
https://www.readbyqxmd.com/read/28264171/role-of-the-luxr-family-transcriptional-regulator-lpg2524-in-the-survival-of-i-legionella-pneumophila-i-in-water
#13
Laam Li, Sébastien P Faucher
The water-borne Gram-negative bacterium <i>Legionella pneumophila</i> (<i>Lp</i>) is the causative agent of Legionnaires' disease. <i>Lp</i> is typically transmitted to human from water systems, where it grows inside amoebae. Survival of <i>Lp</i> in water is central to its transmission to humans. A transcriptomic study previously identified many genes induced by <i>Lp</i> in water. One of such gene, <i>lpg2524</i>, encodes a putative LuxR family transcriptional regulator...
March 6, 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/28261625/isolation-and-identification-of-legionella-spp-from-different-aquatic-sources-in-south-west-of-iran-by-molecular-culture-methods
#14
Mohammad Tabatabaei, Zahra Hemati, Maryam-O-Sadat Moezzi, Negar Azimzadeh
Legionella pneumophila, the causative agent of Legionnaires' diseases (LD) is usually transmitted to humans via inhalation of aerosols from contaminated natural and manmade water sources. These organisms may become fatal especially in immunocompromised patients and LD is the one of the important disease from a public health perspective. This survey investigated the frequency of Legionella spp. including L. pneumophila, in some cold and warm water systems in South-West of Iran by culture and PCR methods. Thirty four water samples were collected from diverse water supply systems...
December 2016: Molecular Biology Research Communications
https://www.readbyqxmd.com/read/28259636/the-typical-presentation-of-an-atypical-pathogen-during-an-outbreak-of-legionnaires-disease-in-vila-franca-de-xira-portugal-2014
#15
A Dias, A Cysneiros, F T Lopes, B von Amann, C Costa, P Dionísio, J Carvalho, V Durão, G Carvalho, F Paula, M Serrado, B Nunes, T Marques, F Froes, C Bárbara
BACKGROUND: An outbreak of Legionella pneumophila serogroup 1, with 403 cases was identified on the 7th November 2014 in Vila Franca de Xira, Portugal. Outbreak source was the wet cooling system of a local factory. Hospital Pulido Valente was one of the hospitals receiving patients with Legionnaires' disease (LD). METHODS: We describe the clinical findings and diagnostic methods used among the 43 confirmed or probable cases admitted to our department. RESULTS: 60...
March 1, 2017: Revista Portuguesa de Pneumologia
https://www.readbyqxmd.com/read/28257493/seeing-red-the-development-of-pon-mcherry-a-broad-host-range-constitutive-expression-plasmid-for-gram-negative-bacteria
#16
Michael J Gebhardt, Rachael K Jacobson, Howard A Shuman
The development of plasmid-mediated gene expression control in bacteria revolutionized the field of bacteriology. Many of these expression control systems rely on the addition of small molecules, generally metabolites or non-metabolized analogs thereof, to the growth medium to induce expression of the genes of interest. The paradigmatic example of an expression control system is the lac system from Escherichia coli, which typically relies on the Ptac promoter and the Lac repressor, LacI. In many cases, however, constitutive gene expression is desired, and other experimental approaches require the coordinated control of multiple genes...
2017: PloS One
https://www.readbyqxmd.com/read/28250292/discrimination-between-legionnaires-disease-and-pneumococcal-pneumonia-based-on-the-clinical-and-laboratory-features-a-quantitative-approach-using-the-modified-winthrop-university-hospital-weighted-point-system
#17
Hiroki Yamakuchi, Yohei Hamada, Tosiharu Urakami, Yosuke Aoki
Objective Legionnaires' disease (LD) is a common form of lobar pneumonia, but the optimum diagnostic modality has long been a subject of debate due to incomplete sensitivity and specificity. A delay in the initiation of specific therapy for LD is associated with increased mortality. The decision to treat a patient for Legionella must be made quickly. The purpose of this study was to evaluate the ability of the modified Winthrop-University Hospital WUH system to identify LD while discriminating against pneumococcal pneumonia at the time of hospitalization for community-acquired pneumonia...
2017: Internal Medicine
https://www.readbyqxmd.com/read/28245548/high-prevalence-and-genetic-polymorphisms-of-legionella-in-natural-and-man-made-aquatic-environments-in-wenzhou-china
#18
Leyi Zhang, Yi Li, Xin Wang, Zhihui Shangguan, Haijian Zhou, Yuejin Wu, Lianghuai Wang, Hongyu Ren, Yun Hu, Meifen Lin, Tian Qin
Natural and engineered water systems are the main sources of Legionnaires' disease. It is essential from a public health perspective to survey water environments for the existence of Legionella. To analyze the main serogroups, genotypes and pathogenicity of the pathogen, a stratified sampling method was adopted to collect water samples randomly from shower water, cooling tower water, and local public hot springs in Wenzhou, China. Suspected strains were isolated from concentrated water samples. Serum agglutination assay and real-time PCR (Polymerase chain reaction) were used to identify L...
February 24, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28217110/intra-species-and-inter-kingdom-signaling-of-legionella-pneumophila
#19
REVIEW
Ramon Hochstrasser, Hubert Hilbi
The ubiquitous Gram-negative bacterium Legionella pneumophila parasitizes environ mental amoebae and, upon inhalation, replicates in alveolar macrophages, thus causing a life-threatening pneumonia called "Legionnaires' disease." The opportunistic pathogen employs a bi-phasic life cycle, alternating between a replicative, non-virulent phase and a stationary, transmissive/virulent phase. L. pneumophila employs the Lqs (Legionella quorum sensing) system as a major regulator of the growth phase switch. The Lqs system comprises the autoinducer synthase LqsA, the homologous sensor kinases LqsS and LqsT, as well as a prototypic response regulator termed LqsR...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28204479/antimicrobial-strategy-for-severe-community-acquired-legionnaires-disease-a-multicentre-retrospective-observational-study
#20
Jérôme Cecchini, Samuel Tuffet, Romain Sonneville, Muriel Fartoukh, Julien Mayaux, Damien Roux, Achille Kouatchet, Florence Boissier, Martial Tchir, Martial Thyrault, Eric Maury, Sebastien Jochmans, Armand Mekontso Dessap, Christian Brun-Buisson, Nicolas de Prost
No abstract text is available yet for this article.
February 13, 2017: Journal of Antimicrobial Chemotherapy
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