keyword
MENU ▼
Read by QxMD icon Read
search

Legionella

keyword
https://www.readbyqxmd.com/read/28917709/in-vitro-interactions-of-acanthamoeba-castellanii-neff-and-vibrio-harveyi
#1
María Reyes-Batlle, Alberto J Martín-Rodríguez, Atteneri López-Arencibia, Ines Sifaoui, Aitor Rizo Liendo, Carlos J Bethencourt Estrella, Ana B García Méndez, Olfa Chiboub, Soumaya Hajaji, Basilio Valladares, Enrique Martínez-Carretero, José E Piñero, Jacob Lorenzo-Morales
Free-living amoebae (FLA) are opportunistic protozoa widely distributed in the environment. They are frequently found in water and soil samples, but they have also been reported to be associated with bacterial human pathogens such as Legionella spp. Campylobacter spp or Vibrio cholerae among others. Including within Vibrio spp. V. harveyi (Johnson and Shunk, 1936) is a bioluminescent marine bacteria which has been found swimming freely in tropical marine waters, being part of the stomach and intestine microflora of marine animals, and as both a primary and opportunistic pathogen of marine animals...
September 13, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28917570/detection-of-viable-but-nonculturable-legionella-in-hospital-water-network-following-monochloramine-disinfection
#2
Beatrice Casini, Angelo Baggiani, Michele Totaro, Antonella Mansi, Anna Laura Costa, Francesco Aquino, Mario Miccoli, Paola Valentini, Fabrizio Bruschi, Pier Luigi Lopalco, Gaetano Privitera
BACKGROUND: Prevention of legionellosis still remains a critical issue in healthcare settings where monochloramine (MC) disinfection was recently introduced as a good alternative to chlorine dioxide in controlling Legionella spp. contamination of the hospital water network. Continuous treatments with low MC doses in some instances have induced a viable but non-culturable state (VBNC) of Legionella spp. AIM: We investigated the occurrence of such dormant cells during a long period of continuous MC treatment...
September 13, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/28914802/phylogenetic-analysis-and-antimicrobial-profiles-of-cultured-emerging-opportunistic-pathogens-phyla-actinobacteria-and-proteobacteria-identified-in-hot-springs
#3
Jocelyn Leonie Jardine, Akebe Luther King Abia, Vuyo Mavumengwana, Eunice Ubomba-Jaswa
Hot spring water may harbour emerging waterborne opportunistic pathogens that can cause infections in humans. We have investigated the diversity and antimicrobial resistance of culturable emerging and opportunistic bacterial pathogens, in water and sediment of hot springs located in Limpopo, South Africa. Aerobic bacteria were cultured and identified using 16S ribosomal DNA (rDNA) gene sequencing. The presence of Legionella spp. was investigated using real-time polymerase chain reaction. Isolates were tested for resistance to ten antibiotics representing six different classes: β-lactam (carbenicillin), aminoglycosides (gentamycin, kanamycin, streptomycin), tetracycline, amphenicols (chloramphenicol, ceftriaxone), sulphonamides (co-trimoxazole) and quinolones (nalidixic acid, norfloxacin)...
September 15, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28912547/a-systematic-exploration-of-the-interactions-between-bacterial-effector-proteins-and-host-cell-membranes
#4
Bethany A Weigele, Robert C Orchard, Alyssa Jimenez, Gregory W Cox, Neal M Alto
Membrane-bound organelles serve as platforms for the assembly of multi-protein complexes that function as hubs of signal transduction in eukaryotic cells. Microbial pathogens have evolved virulence factors that reprogram these host signaling responses, but the underlying molecular mechanisms are poorly understood. Here we test the ability of ~200 type III and type IV effector proteins from six Gram-negative bacterial species to interact with the eukaryotic plasma membrane and intracellular organelles. We show that over 30% of the effectors localize to yeast and mammalian cell membranes, including a subset of previously uncharacterized Legionella effectors that appear to be able to regulate yeast vacuolar fusion...
September 14, 2017: Nature Communications
https://www.readbyqxmd.com/read/28910630/fragment-and-conquer
#5
Vera Kozjak-Pavlovic, Suvagata Roy Chowdhury, Thomas Rudel
The replication vacuole of Legionella pneumophila makes contact with host mitochondria. In this issue of Cell Host & Microbe, Escoll et al. (2017) dissect the mechanisms of this interaction, the effect of the T4SS effector MitF on mitochondrial function, and the resultant metabolic reprogramming of infected cells to benefit the bacteria.
September 13, 2017: Cell Host & Microbe
https://www.readbyqxmd.com/read/28900525/a-case-of-legionella-pneumophila-evaluated-with-ct-and-ultrasound
#6
Alessio D'Angelo, Chiara De Simone, Marco Pagnottella, Stefano Rossi, Raffaele Pepe, Giacomo Ruggieri, Giulio Cocco, Cosima Schiavone
A 36-year-old man was admitted to the emergency department of "SS Annunziata" hospital in Chieti complaining of a sharp chest pain arisen some hours before admission. On examination, the patient looked sweaty; his vital signs showed tachycardia and augmented breath rate; sinus tachycardia and normal ventricular repolarization were observed on ECG, and no abnormalities were observed in the echoscan of the hearth. According to the clinical and electrocardiographic findings, and to previous episode of DVT in anamnesis, a thorax CT scan was performed in order to rule out pulmonary embolism...
September 2017: Journal of Ultrasound
https://www.readbyqxmd.com/read/28900358/risk-assessment-of-legionellosis-in-cardiology-units
#7
REVIEW
P Laganà, S Delia, E Avventuroso, M Casale, G Dattilo
Infective Endocarditis (IE) is a disease with high morbidity and mortality. Nowadays, in addition to classic pathogens were isolated exigent Gram negative bacteria as A. baumannii, A. lwoffii, C. burnetii, Bartonella, Chlamydia and Legionella. We present our experience of Legionella isolations in environmental sample (water and air) collected from the Cardiology units belonging to two hospitals in Messina (Italy). A total of 80 samples were carried out, 30 and 50, respectively in the first and in the second structure: 55 of water and 25 of aerosol...
June 2017: Journal of Preventive Medicine and Hygiene
https://www.readbyqxmd.com/read/28890505/a-case-of-community-acquired-pneumonia-due-to-legionella-pneumophila-serogroup-9-in-which-initial-treatment-with-single-dose-oral-azithromycin-appeared-useful
#8
Akihiro Ito, Tadashi Ishida, Hiromasa Tachibana, Yuhei Ito, Takuya Takaiwa, Hiroyuki Fujii, Toru Hashimoto, Hiroshi Nakajima, Junko Amemura-Maekawa
Legionella species are important causative pathogens for severe community-acquired pneumonia (CAP). Most cases of Legionella pneumonia are due to Legionella pneumophila serogroup 1, and CAP due to Legionella pneumophila serogroup 9 is rare. A fourth case of CAP due to Legionella pneumophila serogroup 9 is reported, and initial treatment using single-dose oral azithromycin appeared useful. Azithromycin injection or fluoroquinolone injection is usually recommended for the treatment of Legionella pneumonia, and no previous reports have shown the effectiveness of single-dose oral azithromycin...
September 11, 2017: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28890285/the-rate-of-legionella-pneumophila-colonization-in-hospital-hot-water-network-after-time-flow-taps-installation
#9
M Totaro, P Valentini B, A L Costa, S Giorgi, B Casini, A Baggiani
In hospital water systems legionellae may be resistant to disinfectants in pipework, which is a problem particularly in areas where there is low flo or stagnation of water. We evaluated legionella colonization of a water network of an Italian hospital after time flow taps (TFTs) installation in proximity to dead legs. The water volume flushed was 64 L/day from May 2016, and 192 L/day from December 2016. Before TFTs installation, Legionella pneumophila sg2-14 was detected in all points (4x10(4)±3.1x10(4)CFU/L)...
September 7, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/28890039/legionella-longbeachae-pneumonia-as-a-complication-of-alemtuzumab-therapy
#10
S Boyle, G Olive, N Townell, A Henderson, S Bowler, S Blum
No abstract text is available yet for this article.
September 8, 2017: Journal of Clinical Neuroscience: Official Journal of the Neurosurgical Society of Australasia
https://www.readbyqxmd.com/read/28889040/greywater-reuse-assessment-of-the-health-risk-induced-by-legionella-pneumophila
#11
Marina Blanky, Yehonatan Sharaby, Sara Rodríguez-Martínez, Malka Halpern, Eran Friedler
Greywater (GW), domestic wastewater excluding the streams generated by toilets and kitchens, can serve as an alternative water source. The main options for GW reuse are toilet flushing and garden irrigation, both generating aerosols. These may transmit inhalable pathogens like Legionella and present a potential health risk. This study quantified the health risk that may arise from inhalation of Legionella-contaminated aerosols due to non-potable GW reuse. Data on Legionella concentrations in potable water and GW was collected...
August 30, 2017: Water Research
https://www.readbyqxmd.com/read/28887725/recombinant-flagellin-pal-fusion-protein-of-legionella-pneumophila-induced-cell-mediated-and-protective-immunity-against-bacteremia-in-balb-c-mice
#12
Ashraf Mohabati Mobarez, Roya Ahmadrajabi, Nima Khoramabadi, Ali Hatef Salmanian
We report a new recombinant fusion protein composed of full-length Legionella pneumophila flagellin A and peptidoglycan-associated lipoprotein (PAL), rFLA-PAL, capable of inducing protective immunity against L. pneumophila. The recombinant protein was over expressed in Escherichia coli strain BL21 (DE3) using pET-28a (+) expression vector (pET28a-flaA-pal) and purified by Ni(2+) exchange chromatography. Immunological properties of rFLA-PAL were assessed in a mouse model. Female BALB/c mice, immunized with rFLA-PAL, exhibited a rapid increase in serum antibody concentration against each of its protein portions...
September 8, 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28887059/toll-like-receptor-2-and-its-roles-in-immune-responses-against-legionella-pneumophila
#13
REVIEW
Shiva Ahmadishoar, Ashraf Kariminik
Legionella pneumophila (L. pneumophila) is an intracellular bacterium which can be survived in the human macrophages phagosomes. The infectious agent is cleared in some cases and survived in others. The main mechanisms responsible for survival of L. pneumophila are yet to be clarified. It has been reported that innate immunity plays key roles in limitation and also eradication of bacterial infections. Toll like receptor 2 (TLR2) is an important cell membrane receptor which recognizes a wide range of bacterial antigens entitled pathogen associated molecular patterns (PAMPs)...
September 5, 2017: Life Sciences
https://www.readbyqxmd.com/read/28883006/acute-fibrinous-and-organising-pneumonia
#14
João Rocha Gonçalves, Ricardo Marques, Paula Serra, Leila Cardoso
Acute fibrinous and organising pneumonia (AFOP) is a rare histological pattern of interstitial lung disease. The authors describe a 60-year-old woman admitted to the hospital for sustained fever, presenting with an alveolar opacity on chest X-ray, with the presumed diagnosis of community-acquired pneumonia and the onset of antibiotics. Since serological results suggested that Legionella pneumophila was the infectious agent, she was discharged on levofloxacin. A week later, she was again admitted with fever...
September 7, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28882518/viability-qpcr-a-new-tool-for-legionella-risk-management
#15
X Lizana, A López, S Benito, G Agustí, M Ríos, N Piqué, A M Marqués, F Codony
BACKGROUND: Viability quantitative Polymerase Chain Reaction (v-qPCR) is a recent analytical approach for only detecting live microorganisms by DNA amplification-based methods This approach is based on the use of a reagent that irreversibly fixes dead cells DNA. In this study, we evaluate the utility of v-qPCR versus culture method for Legionellosis risk management. METHODS: The present study was performed using 116 real samples. Water samples were simultaneously analysed by culture, v-qPCR and qPCR methods...
September 1, 2017: International Journal of Hygiene and Environmental Health
https://www.readbyqxmd.com/read/28878740/dna-delivery-and-genomic-integration-into-mammalian-target-cells-through-type-iv-a-and-b-secretion-systems-of-human-pathogens
#16
Dolores L Guzmán-Herrador, Samuel Steiner, Anabel Alperi, Coral González-Prieto, Craig R Roy, Matxalen Llosa
We explore the potential of bacterial secretion systems as tools for genomic modification of human cells. We previously showed that foreign DNA can be introduced into human cells through the Type IV A secretion system of the human pathogen Bartonella henselae. Moreover, the DNA is delivered covalently attached to the conjugative relaxase TrwC, which promotes its integration into the recipient genome. In this work, we report that this tool can be adapted to other target cells by using different relaxases and secretion systems...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28875940/subversion-of-macrophage-functions-by-bacterial-protein-toxins-and-effectors
#17
Muyang Wan, Yan Zhou, Yongqun Zhu
Macrophages represent one of the first lines of host immune defenses against the invasion of pathogenic bacteria. Many receptors, immune signaling pathways and cellular processes in macrophages, including Toll-like receptors, Nod-like receptors, phagocytosis, autophagy and programmed cell death, are involved in combating the infection of bacterial pathogens. For efficient colonization in the host, bacterial pathogens have evolved diverse mechanisms to interfere with macrophage functions to evade host defenses...
September 6, 2017: Current Issues in Molecular Biology
https://www.readbyqxmd.com/read/28875372/comparison-of-ema-pma-and-dnase-qpcr-for-the-determination-of-microbial-cell-viability
#18
B Reyneke, T Ndlovu, S Khan, W Khan
Ethidium monoazide (EMA) quantitative polymerase chain reaction (qPCR), propidium monoazide (PMA)-qPCR and DNase treatment in combination with qPCR were compared for the determination of microbial cell viability. Additionally, varying EMA and PMA concentrations were analysed to determine which dye and concentration allowed for the optimal identification of viable cells. Viable, heat treated (70 °C for 15 min) and autoclaved cultures of Legionella pneumophila, Pseudomonas aeruginosa, Salmonella typhimurium, Staphylococcus aureus and Enterococcus faecalis were utilised in the respective viability assays...
September 5, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28873330/differential-expression-of-virulence-genes-in-legionella-pneumophila-growing-in-acanthamoeba-and-human-monocytes
#19
Qianqian Mou, Polly H M Leung
Legionella pneumophila, the causative agent of Legionnaires' disease, is widely distributed throughout natural and artificial water systems and can replicate in macrophages and amoebae. Amoebae are the natural hosts of L. pneumophila, whereas macrophages are incidentally infected. The life cycle of L. pneumophila comprises a replicative phase within the Legionella-containing vacuole (LCV) and a transmissive phase during which bacterial cells become motile and are released via killing of the host. Although the host death mechanisms induced by L...
September 5, 2017: Virulence
https://www.readbyqxmd.com/read/28870277/lessons-learned-from-implementing-an-incident-command-system-during-a-local-multiagency-response-to-a-legionnaires-disease-cluster-in-sydney-nsw
#20
Emma Quinn, Travers Johnstone, Zeina Najjar, Toni Cains, Geoff Tan, Essi Huhtinen, Sven Nilsson, Stuart Burgess, Matthew Dunn, Leena Gupta
The incident command system (ICS) provides a common structure to control and coordinate an emergency response, regardless of scale or predicted impact. The lessons learned from the application of an ICS for large infectious disease outbreaks are documented. However, there is scant evidence on the application of an ICS to manage a local multiagency response to a disease cluster with environmental health risks. The Sydney Local Health District Public Health Unit (PHU) in New South Wales, Australia, was notified of 5 cases of Legionnaires' disease during 2 weeks in May 2016...
September 5, 2017: Disaster Medicine and Public Health Preparedness
keyword
keyword
21124
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"