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https://www.readbyqxmd.com/read/28817941/-a-case-of-severe-legionella-longbeachae-pneumonia-and-usefulness-of-lamp-assay
#1
Kumiko Matsushita, Kohei Hijikuro, Shohei Arita, Yu Kaneko, Masahiro Isozaki
Urinary antigen test is frequently used as a routine laboratory test for early diagnosis of Legionella infection, which is especially suitable for ordinary Legionella pneumophila serogroup 1, but not for other types of Legionella. We report a case of severe pneumonia caused by Legionella longbeachae, where a method of loop-mediated isothermal amplification (LAMP) assay contributed an important role for the early detection. This case involved an 83-year-old man who developed fever, dyspnea, and productive cough...
August 15, 2017: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://www.readbyqxmd.com/read/28815533/hijacking-of-membrane-contact-sites-by-intracellular-bacterial-pathogens
#2
Isabelle Derré
Intracellular bacterial pathogens have evolved sophisticated mechanisms to hijack host cellular processes to promote their survival and replication inside host cells. Over the past two decades, much attention has been given to the strategies employed by these pathogens to manipulate various vesicular trafficking pathways. But in the past 5 years, studies have brought to light that intracellular bacteria also target non-vesicular trafficking pathways. Here we review how three vacuolar pathogens, namely, Legionella, Chlamydia, and Coxiella hijack components of cellular MCS with or without the formation of stable MCS...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28809541/ubiquitin-chains-modified-by-the-bacterial-ligase-sdea-are-protected-from-deubiquitinase-hydrolysis
#3
Kedar Puvar, Yiyang Zhou, Jiazhang Qiu, Zhao-Qing Luo, Mary J Wirth, Chittaranjan Das
The SidE family of Legionella pneumophila effectors is a unique group of ubiquitin-modifying enzymes. Along with catalyzing NAD(+)-dependent ubiquitination of certain host proteins independent of the canonical E1/E2/E3 pathway, they have also been shown to produce phosphoribosylated free ubiquitin. This modified ubiquitin product is incompatible with conventional E1/E2/E3 ubiquitination processes, with the potential to lock down various cellular functions that are dependent on ubiquitin signaling. Here, we show that in addition to free ubiquitin, Lys63-, Lys48-, Lys11-, and Met1-linked diubiquitin chains are also modified by SdeA in a similar fashion...
August 18, 2017: Biochemistry
https://www.readbyqxmd.com/read/28807095/simultaneous-detection-of-13-key-bacterial-respiratory-pathogens-by-combination-of-multiplex-pcr-and-capillary-electrophoresis
#4
Lu Xi Jiang, Hong Yu Ren, Hai Jian Zhou, Si Hong Zhao, Bo Yan Hou, Jian Ping Yan, Tian Qin, Yu Chen
OBJECTIVE: Lower respiratory tract infections continue to pose a significant threat to human health. It is important to accurately and rapidly detect respiratory bacteria. To compensate for the limits of current respiratory bacteria detection methods, we developed a combination of multiplex polymerase chain reaction (PCR) and capillary electrophoresis (MPCE) assay to detect thirteen bacterial pathogens responsible for lower respiratory tract infections, including Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, Mycoplasma pneumoniae, Legionella spp...
August 2017: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/28800078/response-to-comments-on-montagna-et-al-evaluation-of-legionella-air-contamination-in-healthcare-facilities-by-different-sampling-methods-an-italian-multicenter-study-int-j-environ-res-public-health-2017-14-670
#5
Maria Teresa Montagna, Osvalda De Giglio, Antonella Agodi, Cesira Pasquarella
We would like to thank Collins andWalker for their comments and for acknowledging that this is an area requiring more research to improve our basic understanding of Legionella [1]. [...].
August 11, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28782709/mic-distribution-among-wild-type-strains-of-legionella-pneumophila-identifies-a-subpopulation-with-reduced-susceptibility-to-macrolides-owing-to-efflux-pump-genes
#6
Marine Vandewalle-Capo, Clémence Massip, Ghislaine Descours, Joséphine Charavit, Joelle Chastang, Pierre Alain Billy, Sandrine Boisset, Gerard Lina, Christophe Gilbert, Max Maurin, Sophie Jarraud, Christophe Ginevra
Legionnaires' disease is a severe pneumonia mainly caused by Legionella pneumophila, and which is treated by antibiotics. The purpose of this study was to describe the susceptibility of clinical strains of Legionella pneumophila to seven antibiotics used for legionellosis treatment. The minimum inhibitory concentrations (MICs) of 109 well-characterized clinical strains of L. pneumophila serogroup-1 were determined using a broth microdilution method without charcoal and compared to antibiotic-resistant strains selected in vitro...
August 4, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28780244/legionella-pneumophila-lps-to-evaluate-urinary-antigen-tests
#7
Anne-Gaëlle Ranc, Margot Carpentier, Laëtitia Beraud, Ghislaine Descours, Christophe Ginevra, Elodie Maisonneuve, Julien Verdon, Jean-Marc Berjeaud, Gérard Lina, Sophie Jarraud
Three urinary antigen tests were compared using purified Legionella pneumophila (Lp) LPS. For Lp serogroup1, Sofia®FIA and Binax®EIA limits of detection (LOD) were similar; that of BinaxNOW® lower. For all tests the LOD was higher with LPS from non-Pontiac compared to Pontiac-strains. The LOD was variable for other Lp serogroups.
June 23, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28777305/comment-on-montagna-et-al-evaluation-of-legionella-air-contamination-in-healthcare-facilities-by-different-sampling-methods-an-italian-multicenter-study-int-j-environ-res-public-health-2017-14-670
#8
Samuel Collins, Jimmy Walker
No abstract text is available yet for this article.
August 4, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28776986/crystal-structure-and-biochemical-characterization-of-an-aminopeptidase-lapb-from-legionella-pneumophila
#9
Nannan Zhang, Shiyan Yin, Wei Zhang, Xiaojian Gong, Na Zhang, Kai Fang, Honghua Ge
Aminopeptidases are a group of exopeptidases that catalyze the removal of a wide range of N-terminal amino acid residues from peptides and proteins. They have many important commercial applications in the food industry. We determined the crystal structure of an aminopeptidase LapB from Legionella pneumophila. The overall structure reveals that the N-terminal protease-associated (PA) domain presents a new fold and shields the active site cavity of the conserved C-terminal peptidase domain. The steady-state kinetic analysis of LapB and the PA domain deletion mutant indicate that the PA domain inhibited enzyme activity of the peptidase domain...
August 17, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28776042/characterization-of-legionella-species-from-watersheds-in-british-columbia-canada
#10
Michael A Peabody, Jason A Caravas, Shatavia S Morrison, Jeffrey W Mercante, Natalie A Prystajecky, Brian H Raphael, Fiona S L Brinkman
Legionella spp. present in some human-made water systems can cause Legionnaires' disease in susceptible individuals. Although legionellae have been isolated from the natural environment, variations in the organism's abundance over time and its relationship to aquatic microbiota are poorly understood. Here, we investigated the presence and diversity of legionellae through 16S rRNA gene amplicon and metagenomic sequencing of DNA from isolates collected from seven sites in three watersheds with varied land uses over a period of 1 year...
July 2017: MSphere
https://www.readbyqxmd.com/read/28771586/inhibition-of-caspase-1-or-gasdermin-d-enable-caspase-8-activation-in-the-naip5-nlrc4-asc-inflammasome
#11
Danielle P A Mascarenhas, Daiane M Cerqueira, Marcelo S F Pereira, Fernanda V S Castanheira, Talita D Fernandes, Graziele Z Manin, Larissa D Cunha, Dario S Zamboni
Legionella pneumophila is a Gram-negative, flagellated bacterium that survives in phagocytes and causes Legionnaires' disease. Upon infection of mammalian macrophages, cytosolic flagellin triggers the activation of Naip/NLRC4 inflammasome, which culminates in pyroptosis and restriction of bacterial replication. Although NLRC4 and caspase-1 participate in the same inflammasome, Nlrc4-/- mice and their macrophages are more permissive to L. pneumophila replication compared with Casp1/11-/-. This feature supports the existence of a pathway that is NLRC4-dependent and caspase-1/11-independent...
August 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28769899/legionella-persistence-in-manufactured-water-systems-pasteurization-potentially-selecting-for-thermal-tolerance
#12
REVIEW
Harriet Whiley, Richard Bentham, Melissa H Brown
Legionella is an opportunistic waterborne pathogen of increasing public health significance. Pasteurization, otherwise known as super-heat and flush (increasing water temperature to above 70°C and flushing all outlets), has been identified as an important mechanism for the disinfection of Legionella in manufactured water systems. However, several studies have reported that this procedure was ineffective at remediating water distribution systems as Legionella was able to maintain long term persistent contamination...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28768406/community-acquired-legionnaires-disease-in-a-newly-constructed-apartment-building
#13
Sukhyun Ryu, Kyungho Yang, Byung Chul Chun
OBJECTIVES: Legionnaires' disease (LD) is a severe type of pneumonia caused by inhalation of aerosols contaminated with Legionella. On September 22, 2016, a single case of LD was reported from a newly built apartment building in Gyeonggi province. This article describes an epidemiologic investigation of LD and identification of the possible source of infection. METHODS: To identify the source of LD, we interviewed the patient's husband using a questionnaire based on the Legionella management guidelines from the Korea Centers for Disease Control and Prevention...
July 2017: Journal of Preventive Medicine and Public Health, Yebang Ŭihakhoe Chi
https://www.readbyqxmd.com/read/28761819/legionella-pneumophila-and-dendrimers-mediated-antisense-therapy
#14
REVIEW
Roghiyeh Pashaei-Asl, Khodadad Khodadadi, Fatima Pashaei-Asl, Gholamreza Haqshenas, Nasser Ahmadian, Maryam Pashaiasl, Reza Hajihosseini Baghdadabadi
Finding novel and effective antibiotics for treatment of Legionella disease is a challenging field. Treatment with antibiotics usually cures Legionella infection; however, if the resultant disease is not timely recognized and treated properly, it leads to poor prognosis and high case fatality rate. Legionella pneumophila DrrA protein (Defects in Rab1 recruitment protein A)/also known as SidM affects host cell vesicular trafficking through modification of the activity of cellular small guanosine triphosphatase )GTPase( Rab (Ras-related in brain) function which facilitates intracellular bacterial replication within a supporter vacuole...
June 2017: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28756828/is-google-trends-a-reliable-tool-for-digital-epidemiology-insights-from-different-clinical-settings
#15
Gianfranco Cervellin, Ivan Comelli, Giuseppe Lippi
Internet-derived information has been recently recognized as a valuable tool for epidemiological investigation. Google Trends, a Google Inc. portal, generates data on geographical and temporal patterns according to specified keywords. The aim of this study was to compare the reliability of Google Trends in different clinical settings, for both common diseases with lower media coverage, and for less common diseases attracting major media coverage. We carried out a search in Google Trends using the keywords "renal colic", "epistaxis", and "mushroom poisoning", selected on the basis of available and reliable epidemiological data...
September 2017: Journal of Epidemiology and Global Health
https://www.readbyqxmd.com/read/28753503/disinfection-of-various-bacterial-pathogens-using-novel-silver-nanoparticle-decorated-magnetic-hybrid-colloids
#16
SungJun Park, Hye Hun Park, Young-Seon Ko, Su Jin Lee, The Son Le, Kyoungja Woo, GwangPyo Ko
Silver nanoparticles (AgNPs) have long been considered a powerful disinfectant for controlling pathogenic microorganisms. However, AgNPs might have adverse effects on both human health and our ecosystems due to their potential cytotoxicity and the difficulty in recovering them after their release into the environment. In this study, we characterized the antimicrobial efficacy caused by a novel micrometer-sized magnetic hybrid colloid (MHC) containing 7, 15, or 30nm sized monodispersed AgNPs (AgNP-MHCs), which can be re-collected from the environment using simple procedures, such as a magnet or centrifugation...
July 24, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28746866/aim2-engages-active-but-unprocessed-caspase-1-to-induce-noncanonical-activation-of-the-nlrp3-inflammasome
#17
Larissa D Cunha, Alexandre L N Silva, Juliana M Ribeiro, Danielle P A Mascarenhas, Gustavo F S Quirino, Leonardo L Santos, Richard A Flavell, Dario S Zamboni
Inflammasomes are multimeric protein complexes that initiate inflammatory cascades. Their activation is a hallmark of many infectious or inflammatory diseases. Their composition and activity are specified by proinflammatory stimuli. For example, the NLRP3 inflammasome is activated in response to cell damage and K(+) efflux, whereas the AIM2 inflammasome is activated in response to cytosolic DNA. We used Legionella pneumophila, an intracellular bacterial pathogen that activates multiple inflammasomes, to elucidate the molecular mechanisms regulating inflammasome activation during infection...
July 25, 2017: Cell Reports
https://www.readbyqxmd.com/read/28740179/legionella-pneumophila-infection-activates-bystander-cells-differentially-by-bacterial-and-host-cell-vesicles
#18
Anna Lena Jung, Christina Elena Herkt, Christine Schulz, Kathrin Bolte, Kerstin Seidel, Nicoletta Scheller, Alexandra Sittka-Stark, Wilhelm Bertrams, Bernd Schmeck
Extracellular vesicles from eukaryotic cells and outer membrane vesicles (OMVs) released from gram-negative bacteria have been described as mediators of pathogen-host interaction and intercellular communication. Legionella pneumophila (L. pneumophila) is a causative agent of severe pneumonia. The differential effect of bacterial and host cell vesicles in L. pneumophila infection is unknown so far. We infected THP-1-derived or primary human macrophages with L. pneumophila and isolated supernatant vesicles by differential centrifugation...
July 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28724535/priming-in-a-permissive-type-i-c-crispr-cas-system-reveals-distinct-dynamics-of-spacer-acquisition-and-loss
#19
Chitong Rao, Denny Chin, Alexander Wilson Ensminger
CRISPR-Cas is a bacterial and archaeal adaptive immune system that uses short, invader-derived sequences termed spacers to target invasive nucleic acids. Upon recognition of previously encountered invaders, the system can stimulate secondary spacer acquisitions, a process known as primed adaptation. Previous studies of primed adaptation have been complicated by intrinsically high interference efficiency of most systems against bona fide targets. As such, most primed adaptation to date has been studied within the context of imperfect sequence complementarity between spacers and targets...
July 19, 2017: RNA
https://www.readbyqxmd.com/read/28724206/vital-signs-health-care-associated-legionnaires-disease-surveillance-data-from-20-states-and-a-large-metropolitan-area-united-states-2015
#20
EDITORIAL
E A Soda, A E Barskey, P P Shah, S Schrag, C G Whitney, M J Arduino, S C Reddy, J M Kunz, C M Hunter, B H Raphael, L A Cooley
BACKGROUND: Legionnaires' disease, a severe pneumonia, is typically acquired through inhalation of aerosolized water containing Legionella bacteria. Legionella can grow in the complex water systems of buildings, including health care facilities. Effective water management programs could prevent the growth of Legionella in building water systems. METHODS: Using national surveillance data, Legionnaires' disease cases were characterized from the 21 jurisdictions (20 U...
August 2017: American Journal of Transplantation
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