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https://www.readbyqxmd.com/read/28448459/viability-of-legionella-pneumophila-in-water-samples-a-comparison-of-propidium-monoazide-pma-treatment-on-membrane-filters-and-in-liquid
#1
Sara Bonetta, Cristina Pignata, Silvia Bonetta, Lorenza Meucci, Donatella Giacosa, Elena Marino, Giorgio Gilli, Elisabetta Carraro
Legionella pneumophila is a ubiquitous microorganism widely distributed in aquatic environments and can cause Legionellosis in humans. A promising approach to detect viable cells in water samples involves the use of quantitative polymerase chain reaction (qPCR) in combination with photoactivatable DNA intercalator propidium monoazide (PMA). However, the PMA efficiency could be different depending on the experimental conditions used. The aim of this study was to compare two PMA exposure protocols: (A) directly on the membrane filter or (B) in liquid after filter washing...
April 27, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28447136/-environmental-health-relevance-of-airborne-microorganisms-in-ambient-and-indoor-air
#2
REVIEW
Sandra M Walser, Bernhard Brenner, Stefanie Heinze, Regine Szewzyk, Eike Wolter, Caroline E W Herr
Airborne microorganisms occur ubiquitously in the ambient air. Besides allergic and irritative-toxic effects, they can cause infections after inhalation. Occupational studies have shown that an increased incidence of respiratory diseases is found in adequately exposed workers. In addition to respiratory diseases, severe systemic infections can also occur in particular cases, such as in the case of a hantavirus infection that is recognized as an occupational disease. In studies from environmental medicine, respiratory diseases have also been observed in residents living in the vicinity of livestock facilities and evaporative cooling towers...
April 26, 2017: Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz
https://www.readbyqxmd.com/read/28445535/microrna-125a-3p-is-regulated-by-myd88-in-legionella-pneumophila-infection-and-targets-ntan1
#3
Elisa Jentho, Malena Bodden, Christine Schulz, Anna-Lena Jung, Kerstin Seidel, Bernd Schmeck, Wilhelm Bertrams
BACKGROUND: Legionella pneumophila (L. pneumophila) is a causative agent of severe pneumonia. It is highly adapted to intracellular replication and manipulates host cell functions like vesicle trafficking and mRNA translation to its own advantage. However, it is still unknown to what extent microRNAs (miRNAs) are involved in the Legionella-host cell interaction. METHODS: WT and MyD88-/- murine bone marrow-derived macrophages (BMM) were infected with L. pneumophila, the transcriptome was analyzed by high throughput qPCR array (microRNAs) and conventional qPCR (mRNAs), and mRNA-miRNA interaction was validated by luciferase assays with 3´-UTR mutations and western blot...
2017: PloS One
https://www.readbyqxmd.com/read/28441664/protection-against-legionnaire-s-disease-recombinant-flagellin-a-of-legionella-pneumophila-can-induce-protective-immunity-against-bacteremia-in-a-balb-c-murine-model
#4
Ashraf Mohabati Mobarez, Roya Ahmadrajabi, Nima Khoramabadi, Ali-Hatef Salmanian
To investigate the immunoprotective effects of the recombinant type A flagellin (FLA), the flaA gene of Legionella pneumophila serogroup 1 strain Paris was cloned into pET28a(+). Recombinant FLA (rFLA) was overexpressed in E. coli BL21 (DE3) and purified by Ni2+ exchange chromatography. Female BALB/c aged 6-8 weeks were immunized with 20 μg of rFLA. Nonimmunized mice along with mice inoculated with a sublethal dose of live L. pneumophila intravenously were considered as negative and positive controls, respectively...
April 26, 2017: Journal of Molecular Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28438301/typical-presentation-of-legionella-pneumophila-community-acquired-pneumonia
#5
EDITORIAL
C Cilloniz, A Torres
No abstract text is available yet for this article.
May 2017: Revista Portuguesa de Pneumologia
https://www.readbyqxmd.com/read/28420075/application-of-hydrogen-peroxide-as-an-innovative-method-of-treatment-for-legionella-control-in-a-hospital-water-network
#6
Beatrice Casini, Francesco Aquino, Michele Totaro, Mario Miccoli, Irio Galli, Laura Manfredini, Carlo Giustarini, Anna Laura Costa, Benedetta Tuvo, Paola Valentini, Gaetano Privitera, Angelo Baggiani
OBJECTIVES: To evaluate the effectiveness of hydrogen peroxide (HP) use as a disinfectant in the hospital water network for the control of Legionella spp. colonization. METHODS: Following the detection of high levels of Legionella contamination in a 136-bed general hospital water network, an HP treatment of the hot water supply (25 mg/L) was adopted. During a period of 34 months, the effectiveness of HP on Legionella colonization was assessed. Legionella was isolated in accordance with ISO-11731 and identification was carried out by sequencing of the mip gene...
April 17, 2017: Pathogens
https://www.readbyqxmd.com/read/28418314/population-genomics-of-legionella-longbeachae-and-hidden-complexities-of-infection-source-attribution
#7
Rodrigo Bacigalupe, Diane Lindsay, Giles Edwards, J Ross Fitzgerald
Legionella longbeachae is the primary cause of legionellosis in Australasia and Southeast Asia and an emerging pathogen in Europe and the United States; however, our understanding of the population diversity of L. longbeachae from patient and environmental sources is limited. We analyzed the genomes of 64 L. longbeachae isolates, of which 29 were from a cluster of legionellosis cases linked to commercial growing media in Scotland in 2013 and 35 were non-outbreak-associated isolates from Scotland and other countries...
May 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28396241/molecular-typing-of-legionella-pneumophila-from-air-conditioning-cooling-waters-using-mip-gene-sbt-and-faflp-methods
#8
Xiangli Gong, Juntao Li, Ying Zhang, Shuiping Hou, Pinghua Qu, Zhicong Yang, Shouyi Chen
Legionella spp. are important waterborne pathogens. Molecular typing has become an important method for outbreaks investigations and source tracking of Legionnaires. In a survey program conducted by the Guangzhou Center for Disease Control and Prevention, multiple serotypes Legionella pneumophila (L. pneumophila) were isolated from waters in air-conditioning cooling towers in urban Guangzhou region, China between 2008 and 2011. Three genotyping methods, mip (macrophage infectivity potentiator) genotyping, SBT (sequence-based typing), and FAFLP (fluorescent amplified fragment length polymorphism analysis) were used to type these waterborne L...
April 7, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28395732/elucidation-of-the-anti-autophagy-mechanism-of-the-legionella-effector-ravz-using-semisynthetic-lc3-proteins
#9
Aimin Yang, Supansa Pantoom, Yao-Wen Wu
Autophagy is a conserved cellular process involved in the elimination of proteins and organelles. It is also used to combat infection with pathogenic microbes. The intracellular pathogen Legionella pneumophila manipulates autophagy by delivering the effector protein RavZ to deconjugate Atg8/LC3 proteins coupled to phosphatidylethanolamine (PE) on autophagosomal membranes. To understand how RavZ recognizes and deconjugates LC3-PE, we prepared semisynthetic LC3 proteins and elucidated the structures of the RavZ:LC3 interaction...
April 11, 2017: ELife
https://www.readbyqxmd.com/read/28393985/-importance-of-respiratory-virus-in-immunocompetent-adult-patients-hospitalized-with-community-acquired-pneumonia
#10
Fernando Saldías Peñafiel, Marcos Ortega Gutiérrez, Gino Fuentes López, José Manuel Elola Aránguiz, Javier Uribe Monasterio, Arturo Morales Soto, Orlando Díaz Patiño
BACKGROUND: Community-acquired pneumonia (CAP) is a relevant worldwide cause of morbidity and mortality in adult population, however its etiology is often not identified and therapy is empirical. AIM: To assess the etiology of CAP in immunocompetent adult hospitalized patients using conventional and molecular diagnostic methods. MATERIAL AND METHODS: We prospectively studied 240 adult patients who were hospitalized for CAP to identify the microbial etiology...
December 2016: Revista Médica de Chile
https://www.readbyqxmd.com/read/28390237/methodological-approaches-for-monitoring-opportunistic-pathogens-in-premise-plumbing-a-review
#11
REVIEW
Hong Wang, Emilie Bédard, Michèle Prévost, Anne K Camper, Vincent R Hill, Amy Pruden
Opportunistic premise (i.e., building) plumbing pathogens (OPPPs, e.g., Legionella pneumophila, Mycobacterium avium complex, Pseudomonas aeruginosa, Acanthamoeba, and Naegleria fowleri) are a significant and growing source of disease. Because OPPPs establish and grow as part of the native drinking water microbiota, they do not correspond to fecal indicators, presenting a major challenge to standard drinking water monitoring practices. Further, different OPPPs present distinct requirements for sampling, preservation, and analysis, creating an impediment to their parallel detection...
March 25, 2017: Water Research
https://www.readbyqxmd.com/read/28389563/structure-of-the-wipa-protein-reveals-a-novel-tyrosine-protein-phosphatase-effector-from-legionella-pneumophila
#12
Nikos Pinotsis, Gabriel Waksman
Legionnaires disease is a severe form of pneumonia caused by the bacterium Legionella pneumophila. L. pneumophila pathogenicity relies on secretion of more than 300 effector proteins by a type IVb secretion system. Among these Legionella effectors, WipA has been primarily studied because of its dependence on a chaperone complex, IcmSW, for translocation through the secretion system, but its role in pathogenicity has remained unknown. In this study, we present the crystal structure of a large fragment of WipA, WipA435...
April 7, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28384349/neutrophils-and-ly6chi-monocytes-collaborate-in-generating-an-optimal-cytokine-response-that-protects-against-pulmonary-legionella-pneumophila-infection
#13
Cierra N Casson, Jessica L Doerner, Alan M Copenhaver, Jasmine Ramirez, Alicia M Holmgren, Mark A Boyer, Ingharan J Siddarthan, Sara H Rouhanifard, Arjun Raj, Sunny Shin
Early responses mounted by both tissue-resident and recruited innate immune cells are essential for host defense against bacterial pathogens. In particular, both neutrophils and Ly6Chi monocytes are rapidly recruited to sites of infection. While neutrophils and monocytes produce bactericidal molecules, such as reactive nitrogen and oxygen species, both cell types are also capable of synthesizing overlapping sets of cytokines important for host defense. Whether neutrophils and monocytes perform redundant or non-redundant functions in the generation of anti-microbial cytokine responses remains elusive...
April 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28383283/global-analysis-of-gene-expression-reveals-mrna-superinduction-is-required-for-the-inducible-immune-response-to-a-bacterial-pathogen
#14
Kevin C Barry, Nicholas T Ingolia, Russell E Vance
The inducible innate immune response to infection requires a concerted process of gene expression that is regulated at multiple levels. Most global analyses of the innate immune response have focused on transcription induced by defined immunostimulatory ligands, such as lipopolysaccharide. However, the response to pathogens involves additional complexity, as pathogens interfere with virtually every step of gene expression. How cells respond to pathogen-mediated disruption of gene expression to nevertheless initiate protective responses remains unclear...
April 6, 2017: ELife
https://www.readbyqxmd.com/read/28378991/assessment-of-antibiotic-susceptibility-of-legionella-pneumophila-isolated-from-water-systems-in-poland
#15
Agnieszka Sikora, Iwona Gładysz, Maria Kozioł-Montewka, Małgorzata Wójtowicz-Bobin, Tomasz Stańczak, Renata Matuszewska, Bożena Krogulska
INTRODUCTION AND OBJECTIVE: Several studies have reported therapy failures in patients with legionnaires'disease; however, antimicrobial resistance of clinical and environmental isolates of Legionella spp. has not yet been documented. Routine susceptibility testing of Legionella spp. is not recommended because of difficulties in determining standard minimal inhibitory concentration values. The purpose of this study was to analyze the antimicrobial susceptibility of Legionella pneumophila...
March 21, 2017: Annals of Agricultural and Environmental Medicine: AAEM
https://www.readbyqxmd.com/read/28376442/prevalence-of-atypical-pathogens-in-patients-with-cough-and-community-acquired-pneumonia-a-meta-analysis
#16
REVIEW
Christian Marchello, Ariella Perry Dale, Thuy Nhu Thai, Duk Soo Han, Mark H Ebell
PURPOSE: Community-acquired pneumonia (CAP), acute cough, bronchitis, and lower respiratory tract infections (LRTI) are often caused by infections with viruses or Streptococcus pneumoniae. The prevalence of atypical pathogens Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila, and Bordetella pertussis among patients with these illnesses in the ambulatory setting has not been previously summarized. We set out to derive prevalence information from the existing literature...
November 2016: Annals of Family Medicine
https://www.readbyqxmd.com/read/28376265/evaluation-of-legionella-real-time-pcr-against-traditional-culture-for-routine-and-public-health-testing-of-water-samples
#17
Samuel Collins, David Stevenson, Jimmy Walker, Allan Bennett
AIMS: To evaluate the usefulness of Legionella qPCR alongside traditional culture for enumeration of Legionella from water samples as part of both routine and public health investigation testing. METHODS AND RESULTS: Routine water samples (n=2002) and samples from public health investigations (n=215) were analysed by culture and qPCR for Legionella spp., Legionella pneumophila and L. pneumophila sg-1. A negative qPCR result was highly predictive of a negative culture result for all water systems (negative predictive values (NPV) from 97...
April 4, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28373357/the-legionella-pneumophila-incomplete-phosphotransferase-system-pts-is-required-for-optimal-intracellular-growth-and-maximal-expression-of-pmra-regulated-effectors
#18
Yariv Speiser, Tal Zusman, Anna Pasechnek, Gil Segal
The nitrogen phosphotransferase system (PTS(Ntr)) is a regulatory cascade present in many bacteria, where it controls different functions. This system is usually composed of three basic components: EI(Ntr), NPr and EIIA(Ntr) (encoded by the ptsP, ptsO and ptsN genes, respectively). In Legionella pneumophila, as well as in many other Legionella species, the EIIA(Ntr) component is missing. However, we found that deletion mutations in both ptsP and ptsO are partially attenuated for intracellular growth. Furthermore, these two PTS(Ntr) components were found to be required for maximal expression of effector-encoding genes regulated by the transcriptional activator PmrA...
April 3, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28370625/proq-fino-domain-proteins-another-ubiquitous-family-of-rna-matchmakers
#19
REVIEW
Mikolaj Olejniczak, Gisela Storz
Small RNAs (sRNAs), particularly those that act by limited base pairing with mRNAs, are part of most regulatory networks in bacteria. In many cases, the base-pairing interaction is facilitated by the RNA chaperone Hfq. However, not all bacteria encode Hfq and some base-pairing sRNAs do not require Hfq raising the possibility of other RNA chaperones. Candidates are proteins with homology to FinO, a factor that promotes base pairing between the FinP antisense sRNA and the traJ mRNA to control F plasmid transfer...
March 30, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28369254/diving-deep-into-hospital-acquired-legionella-pneumophila-with-whole-genome-sequencing
#20
Scott A Beatson, Paul B Bartley
No abstract text is available yet for this article.
May 1, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
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