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E. Coli O157

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https://www.readbyqxmd.com/read/28213899/human-influence-and-biotic-homogenization-drive-the-distribution-of-escherichia-coli-virulence-genes-in-natural-habitats
#1
Adriana Cabal, Joaquin Vicente, Julio Alvarez, Jose Angel Barasona, Mariana Boadella, Lucas Dominguez, Christian Gortazar
Cattle are the main reservoirs for Shiga-toxin-producing Escherichia coli (STEC), the only known zoonotic intestinal E. coli pathotype. However, there are other intestinal pathotypes that can cause disease in humans, whose presence has been seldom investigated. Thus, our aim was to identify the effects of anthropic pressure and of wild and domestic ungulate abundance on the distribution and diversity of the main human E. coli pathotypes and nine of their representative virulence genes (VGs). We used a quantitative real-time PCR (qPCR) for the direct detection and quantification of the genus-specific gene uidA, nine E...
February 18, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28213539/critical-concentration-of-lecithin-enhances-the-antimicrobial-activity-of-eugenol-against-escherichia-coli
#2
Haoshu Zhang, Edward G Dudley, P Michael Davidson, Federico Harte
Lecithin is a natural emulsifier used in a wide range of food and non-food applications to improve physical stability with no known bioactive effects. In this study, the effect of lecithin on the antimicrobial performance of a constant eugenol concentration was tested against three Escherichia coli strains (C600, 0.1229, and O157:H7 strain ATCC 700728). This was the first study focusing on lecithin at concentrations below those commonly used in foods to improve the stability of oil in water emulsions (≤ 10mg/100ml)...
February 17, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28213035/influence-of-electro-activated-solutions-of-weak-organic-acid-salts-on-microbial-quality-and-overall-appearance-of-blueberries-during-storage
#3
Viacheslav Liato, Riadh Hammami, Mohammed Aïder
The aim of this work was to study the potential of diluted electro-activated solutions of weak organic acid salts (potassium acetate, potassium citrate and calcium lactate) to extend the shelf life of blueberries during post-harvest storage. The sanitizing capacity of these solutions was studied against pathogenic bacteria Listeria monocytogenes and E. coli O157:H7 as well as phytopathogenic fungi A. alternata, F. oxysporum and B. cinerea. The results showed that a 5-min treatment of inoculated blueberries with electro-activated solutions resulted in a 4 log CFU/g reduction in Listeria monocytogenes for all solutions...
June 2017: Food Microbiology
https://www.readbyqxmd.com/read/28213024/sanitizer-efficacy-in-preventing-cross-contamination-of-heads-of-lettuce-during-retail-crisping
#4
Yangjin Jung, Hyein Jang, Mengqi Guo, Jingwen Gao, Karl R Matthews
This study was conducted to provide information regarding mitigation of cross-contamination through the use of sanitizer during crisping at retail outlets. Seven non-inoculated heads and one inoculated head (≈5 log CFU/g) of lettuce were placed into commercial sink filled with 76 L of tap water (TW), electrolyzed water (EW, free chlorine: 43 ± 6 ppm), lactic acid and phosphoric acid-based sanitizer (LPA, pH 2.89), or citric acid-based sanitizer (CA, pH 2.78) and soaked for 5 min. Two subsequent batches (eight non-inoculated heads per batch) were soaked in the same solution...
June 2017: Food Microbiology
https://www.readbyqxmd.com/read/28213023/inactivation-of-escherichia-coli-o157-h7-salmonella-typhimurium-and-listeria-monocytogenes-in-ready-to-bake-cookie-dough-by-gamma-and-electron-beam-irradiation
#5
Seul-Gi Jeong, Dong-Hyun Kang
This study was conducted to investigate the efficacy of gamma and electron beam irradiation to inactivate foodborne pathogens in ready-to-bake cookie dough and to determine the effect on quality by measuring color and texture changes. Cookie dough inoculated with Escherichia coli O157:H7, Salmonella Typhimurium, or Listeria monocytogenes was subjected to gamma and electron beam irradiation, with doses ranging from 0 to 3 kGy. As the radiation dose increased, the inactivation effect increased among all tested pathogens...
June 2017: Food Microbiology
https://www.readbyqxmd.com/read/28207312/effect-of-water-hardness-on-efficacy-of-sodium-hypochlorite-inactivation-of-escherichia-coli-o157-h7-in-water
#6
Sara Swanson, Tong-Jen Fu
This study examined how the hardness of water affected the efficacy of sodium hypochlorite in inactivating Escherichia coli O157:H7 in water. Water was prepared at different degrees of total hardness (0, 50, 100, 200, 500, 1,000, 2,000, and 5,000 mg/liter CaCO3). Inactivation was assessed at different levels of free chlorine (0, 0.2, 0.5, and 1.0 ppm) at 2 to 4°C and pH 6.5. Thirty milliliters of chlorinated water was inoculated with 6 log CFU/ml of E. coli O157:H7 and allowed to mix for 3, 10, 20, or 30 s...
February 16, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28207308/prevalence-of-listeria-monocytogenes-in-retail-lightly-pickled-vegetables-and-its-successful-control-at-processing-plants
#7
Masumi Taguchi, Masashi Kanki, Yuko Yamaguchi, Hideichi Inamura, Yosuke Koganei, Tetsuya Sano, Hiromi Nakamura, Hiroshi Asakura
Incidences of food poisoning traced to nonanimal food products have been increasingly reported. One of these was a recent large outbreak of Shiga toxin-producing Escherichia coli (STEC) O157 infection from the consumption of lightly pickled vegetables, indicating the necessity of imposing hygienic controls during manufacturing. However, little is known about the bacterial contamination levels in these minimally processed vegetables. Here we examined the prevalence of STEC, Salmonella spp., and Listeria monocytogenes in 100 lightly pickled vegetable products manufactured at 55 processing factories...
February 16, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28207302/characteristics-of-shiga-toxin-producing-escherichia-coli-o157-in-slaughtered-reindeer-from-northern-finland
#8
Claudio Zweifel, Lisa Fierz, Nicole Cernela, Sauli Laaksonen, Maria Fredriksson-Ahomaa, Roger Stephan
Fecal samples collected from 470 slaughtered reindeer 6 to 7 months of age were screened by real-time PCR (after enrichment) for Shiga toxin genes (stx) and then for Escherichia coli serogroup O157. Shiga toxin genes were found frequently (>30% of samples), and serogroup O157 was detected in 20% of the stx-positive samples. From these samples, a total of 25 E. coli O157:H(-) isolates (nonmotile but PCR positive for fliCH7) were obtained. Twenty-four of these E. coli O157:H(-) isolates did not ferment sorbitol and originated from one geographic area...
February 16, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28207293/prevalence-of-multidrug-resistant-bacteria-from-u-s-grown-and-imported-fresh-produce-retailed-in-chain-supermarkets-and-ethnic-stores-of-davidson-county-tennessee
#9
Siqin Liu, Agnes Kilonzo-Nthenge
The aim of this study was to determine whether U.S.-grown and imported fresh produce retailed in ethnic stores and chain supermarkets was a reservoir of antibiotic-resistant bacteria. A total of 360 (129 imported and 231 U.S.-grown) samples of fresh produce were purchased from retail stores and analyzed for Enterobacteriaceae , including three pathogenic bacteria ( Escherichia coli O157:H7, Shigella , and Salmonella ), using standard methods. Presumptive pathogenic isolates were confirmed using PCR. The mean Enterobacteriaceae counts for imported produce were 6...
February 16, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28199144/attachment-of-13-types-of-foodborne-bacteria-to-jalape%C3%A3-o-and-serrano-peppers-and-antibacterial-effect-of-roselle-calyx-extracts-sodium-hypochlorite-colloidal-silver-and-acetic-acid-against-these-foodborne-bacteria-on-peppers
#10
Esmeralda Rangel-Vargas, Carlos A Gómez-Aldapa, Reyna N Falfan-Cortes, María L Rodríguez-Marín, Angélica Godínez-Oviedo, Otilio A Acevedo-Sandoval, Javier Castro-Rosas
Chili peppers are a very important crop in Mexico. However, these peppers have been associated with Salmonella infection outbreaks in the United States, and Salmonella and diarrheagenic Escherichia coli pathotypes have been isolated from jalapeño and serrano peppers in Mexico. To decrease microbial contamination of fruits and vegetables, chemical agents are commonly used; however, chemical agents used to eliminate pathogenic bacteria on vegetables have a limited antimicrobial effect. Roselle ( Hibiscus sabdariffa ) calyces have been reported to have an antimicrobial effect on pathogenic bacteria...
February 15, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28197137/different-behavior-of-enteric-bacteria-and-viruses-in-clay-and-sandy-soils-after-biofertilization-with-swine-digestate
#11
Gislaine Fongaro, María C García-González, Marta Hernández, Airton Kunz, Célia R M Barardi, David Rodríguez-Lázaro
Enteric pathogens from biofertilizer can accumulate in the soil, subsequently contaminating water and crops. We evaluated the survival, percolation and leaching of model enteric pathogens in clay and sandy soils after biofertilization with swine digestate: PhiX-174, mengovirus (vMC0), Salmonella enterica Typhimurium and Escherichia coli O157:H7 were used as biomarkers. The survival of vMC0 and PhiX-174 in clay soil was significantly lower than in sandy soil (iT90 values of 10.520 ± 0.600 vs. 21.270 ± 1.100 and 12...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28191041/comparative-genomic-analysis-of-shiga-toxin-producing-and-non-shiga-toxin-producing-escherichia-coli-o157-isolated-from-outbreaks-in-korea
#12
Taesoo Kwon, Won Kim, Seung-Hak Cho
BACKGROUND: The Shiga toxin-producing Escherichia coli (STEC) O157 strain NCCP15739 and non-STEC O157 strain NCCP15738 were isolated from outbreaks in Korea. We characterized NCCP15739 and NCCP15738 by genome sequencing and a comparative genomic analysis using two additional strains, E. coli K-12 substr. MG1655 and O157:H7 EDL933. RESULTS: Using the Illumina HiSeq 2000 platform and the RAST server, the whole genomes of NCCP15739 and NCCP15738 were obtained and annotated...
2017: Gut Pathogens
https://www.readbyqxmd.com/read/28178427/pathogenesis-of-colitis-in-germ-free-mice-infected-with-ehec-o157-h7
#13
K A Eaton, C Fontaine, D I Friedman, N Conti, C J Alteri
Enterohemorrhagic Escherichia coli (EHEC) are strains of E. coli that express Shiga toxins (Stx) and cause hemorrhagic colitis. In some cases, disease can progress to hemolytic uremic syndrome, a potentially fatal form of kidney disease. Both enteric and renal disease are associated with the expression of stx genes, which are often carried on lysogenic phage. Toxin is expressed following induction and conversion of the phage to lytic growth. The authors previously used a germ-free mouse model to demonstrate that toxin gene expression is enhanced during growth in vivo and that renal disease is dependent on both prophage induction and expression of Stx2...
January 1, 2017: Veterinary Pathology
https://www.readbyqxmd.com/read/28178372/antimicrobial-activities-of-phenolic-extracts-derived-from-seed-coats-of-selected-soybean-varieties
#14
Rajaa Abutheraa, Navam Hettiarachchy, Geetha Kumar-Phillips, Ronny Horax, Pengyin Chen, Ruben Morawicki, Young Min Kwon
Soybean hulls or seed coats consist of complex carbohydrates, proteins, lipids, and polyphenols such as anthocyanidins, proanthocyanidins, and isoflavones. The polyphenolics in the seed coats give them various colors such as black, brown, green, yellow, or even a mottled appearance. In this study, the antimicrobial effects of phenolic extracts from the seed coats of different colored soybeans (yellow, dark brown, brown, and black) were evaluated against foodborne pathogens such as Salmonella Typhimurium, Escherichia coli O157:H7, and Campylobacter jejuni in broth-cultures as well as on chicken skin...
February 8, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28174569/identification-and-whole-genome-sequencing-of-the-first-case-of-kosakonia-radicincitans-causing-a-human-bloodstream-infection
#15
Micah D Bhatti, Awdhesh Kalia, Pranoti Sahasrabhojane, Jiwoong Kim, David E Greenberg, Samuel A Shelburne
The taxonomy of Enterobacter species is rapidly changing. Herein we report a bloodstream infection isolate originally identified as Enterobacter cloacae by Vitek2 methodology that we found to be Kosakonia radicincitans using genetic means. Comparative whole genome sequencing of our isolate and other published Kosakonia genomes revealed these organisms lack the AmpC β-lactamase present on the chromosome of Enterobacter sp. A fimbriae operon primarily found in Escherichia coli O157:H7 isolates was present in our organism and other available K...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28169510/differences-in-internalization-and-growth-of-escherichia-coli-o157-h7-within-the-apoplast-of-edible-plants-spinach-and-lettuce-compared-with-the-model-species-nicotiana-benthamiana
#16
Kathryn M Wright, Louise Crozier, Jacqueline Marshall, Bernhard Merget, Ashleigh Holmes, Nicola J Holden
Internalization of food-borne bacteria into edible parts of fresh produce plants represents a serious health risk. Therefore, internalization of verocytotoxigenic E. coli O157:H7 isolate Sakai was assessed in two species associated with outbreaks, spinach (Spinacia oleracea) and lettuce (Lactuca sativa) and compared to the model species Nicotiana benthamiana. Internalization occurred in the leaves and roots of spinach and lettuce throughout a 10 day time-course. The plant species, tissue type and inoculum dose all impacted the outcome...
February 7, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28149486/comparison-of-two-methods-for-detection-of-e-coli-o157h7-in-unpasteurized-milk
#17
Maryam Farahmandfar, Naghmeh Moori-Bakhtiari, Saad Gooraninezhad, Mehdi Zarei
BACKGROUND AND OBJECTIVES: The most common serotype of enterohaemorrhagic Esherichia coli group or Shiga-toxin-producing E. coli is O157:H7. Domestic and wild ruminants are regarded as the main natural reservoirs. O157:H7 serotype is the major cause of gastrointestinal infections in developed countries. In this study was conducted to survey on the toxigenic E. coli O157: H7 strains in milk of industrial dairy farms. MATERIALS AND METHODS: A total number of 150 milk samples were collected from dairy industry in Khuzestan, over a period of 6 months and were evaluated by cultivation in selective media (CT-SMAC) and multiplex PCR...
October 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28141846/changes-in-bacterial-community-composition-of-escherichia-coli-o157-h7-super-shedder-cattle-occur-in-the-lower-intestine
#18
Rahat Zaheer, Eric Dugat-Bony, Devon Holman, Elodie Cousteix, Yong Xu, Krysty Munns, Lorna J Selinger, Rutn Barbieri, Trevor Alexander, Tim A McAllister, L Brent Selinger
Escherichia coli O157:H7 is a foodborne pathogen that colonizes ruminants. Cattle are considered the primary reservoir of E. coli O157:H7 with super-shedders, defined as individuals excreting > 104 E. coli O157:H7 CFU g-1 feces. The mechanisms leading to the super-shedding condition are largely unknown. Here, we used 16S rRNA gene pyrosequencing to examine the composition of the fecal bacterial community in order to investigate changes in the bacterial microbiota at several locations along the digestive tract (from the duodenum to the rectal-anal junction) in 5 steers previously identified as super-shedders and 5 non-shedders...
2017: PloS One
https://www.readbyqxmd.com/read/28122502/occurrence-of-escherichia-coli-o157-h7-in-cattle-feces-and-contamination-of-carcass-and-various-contact-surfaces-in-abattoir-and-butcher-shops-of-hawassa-ethiopia
#19
Biruhtesfa Atnafie, Degmawi Paulos, Mesele Abera, Genene Tefera, Dereje Hailu, Surafel Kasaye, Kebede Amenu
BACKGROUND: Despite of the sanitation measures in municipal abattoirs to reduce contamination, Escherichia coli continues to be a health hazard. The present study was conducted on 150 apparently healthy slaughtered cattle at municipal abattoir and in 50 different butcher shops in Hawassa town, Ethiopia. The objectives of the study were investigating the occurrence and antimicrobial resistance of E. coli O157:H7 isolated from fecal samples, carcasses swab, contacts surfaces (swabs of meat handlers hands, knife and clothes of meat transporters) as well as from butcher shops (meat samples, swabs from cutting board swab, butcher men hand and knife surface)...
January 25, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28120191/candida-krusei-isolated-from-fruit-juices-ultrafiltration-membranes-promotes-colonization-of-escherichia-coli-o157-h7-and-salmonella-enterica-on-stainless-steel-surfaces
#20
María Clara Tarifa, Jorge Enrique Lozano, Lorena Inés Brugnoni
To clarify the interactions between a common food spoilage yeast and two pathogenic bacteria involved in outbreaks associated with fruit juices, the present paper studies the effect of the interplay of Candida krusei, collected from UF membranes, with Escherichia coli O157:H7 and Salmonella enterica in the overall process of adhesion and colonization of abiotic surfaces. Two different cases were tested: a) co-adhesion by pathogenic bacteria and yeasts, and b) incorporation of bacteria to pre-adhered C. krusei cells...
February 2017: Journal of Microbiology / the Microbiological Society of Korea
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