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E. Coli O157

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https://www.readbyqxmd.com/read/28930254/synthesis-antimicrobial-and-antioxidant-activities-of-chalcogen-containing-nitrone-derivatives-from-r-citronellal
#1
Mariana C Ferraz, Renata A Mano, Daniela H Oliveira, Darla S V Maia, Wladimir P Silva, Lucielli Savegnago, Eder J Lenardão, Raquel G Jacob
Background: The main constituents of Cymbopogonnardus (L) Rendle and C. citratus (DC) Stapfessential oils are (R)-citronellal and citral, respectively. Organochalcogen compounds can boost the biological activities of natural products. Methods: Several chalcogen-containing nitrones derived from (R)-citronellal and citral were prepared and evaluated for their antimicrobial and antioxidant activities. The antimicrobial activity was evaluated by the disc diffusion test and the antioxidant properties were evaluated in vitro by DPPH (1,1-diphenyl-2-picryl-hydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), and FRAP (ferric ion reducing antioxidant power) assays...
June 10, 2017: Medicines (Basel, Switzerland)
https://www.readbyqxmd.com/read/28930030/mild-illness-during-outbreak-of-shiga-toxin-producing-escherichia-coli-o157-infections-associated-with-agricultural-show-australia
#2
Bhakti R Vasant, Russell J Stafford, Amy V Jennison, Sonya M Bennett, Robert J Bell, Christine J Doyle, Jeannette R Young, Susan A Vlack, Paul Titmus, Debra El Saadi, Kari A J Jarvinen, Patricia Coward, Janine Barrett, Megan Staples, Rikki M A Graham, Helen V Smith, Stephen B Lambert
During a large outbreak of Shiga toxin-producing Escherichia coli illness associated with an agricultural show in Australia, we used whole-genome sequencing to detect an IS1203v insertion in the Shiga toxin 2c subunit A gene of Shiga toxin-producing E. coli. Our study showed that clinical illness was mild, and hemolytic uremic syndrome was not detected.
October 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28925937/repetitive-immunosensor-with-a-fiber-optic-device-and-antibody-coated-magnetic-beads-for-semi-continuous-monitoring-of-escherichia-coli-o157-h7
#3
Midori Taniguchi, Hirokazu Saito, Kohji Mitsubayashi
A rapid and reproducible fiber-optic immunosensor for Escherichia coli O157:H7 (E. coli O157:H7) was described. The biosensor consisted of a flow cell, an optical fiber with a thin Ni layer, and a PC linked fluorometer. First, the samples with E. coli O157:H7 were incubated with magnetic beads coated with anti-E. coli O157:H7 antibodies and anti-E. coli O157:H7 antibodies labeled cyanine 5 (Cy5) to make sandwich complexes. Then the Cy5-(E. coli O157:H7)-beads were injected into a flow cell and pulled to the magnetized Ni layer on the optical fiber set in the flow cell...
September 19, 2017: Sensors
https://www.readbyqxmd.com/read/28925340/british-escherichia-coli-o157-in-cattle-study-becs-to-determine-the-prevalence-of-e-coli-o157-in-herds-with-cattle-destined-for-the-food-chain
#4
M K Henry, S C Tongue, J Evans, C Webster, I J McKENDRICK, M Morgan, A Willett, A Reeves, R W Humphry, D L Gally, G J Gunn, M E Chase-Topping
Escherichia coli O157 are zoonotic bacteria for which cattle are an important reservoir. Prevalence estimates for E. coli O157 in British cattle for human consumption are over 10 years old. A new baseline is needed to inform current human health risk. The British E. coli O157 in Cattle Study (BECS) ran between September 2014 and November 2015 on 270 farms across Scotland and England & Wales. This is the first study to be conducted contemporaneously across Great Britain, thus enabling comparison between Scotland and England & Wales...
September 19, 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/28922801/effect-of-severe-weather-events-on-the-shedding-of-shiga-toxigenic-escherichia-coli-in-slaughter-cattle-and-phenotype-of-serogroup-o157-isolates
#5
Kim Stanford, Tim Reuter, Susan J Bach, Linda Chui, Angela Ma, Cheyenne C Conrad, Renata Tostes, Tim A McAllister
High-event periods (HEPs) occur sporadically when beef carcasses and meat have episodes of acute contamination with Shiga toxin-producing Escherichia coli (STEC). In this study, severe weather events were investigated as catalysts for HEPs based on PCR and isolate prevalence of seven E. coli serogroups in slaughter cattle feces. Winter ambient temperatures with daily means 10.5oC warmer or 12.3°C colder than seasonal norms (-10.4°C) most altered STEC shedding. Fecal samples yielded increased proportions (P < 0...
September 1, 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/28921940/attribution-of-human-infections-with-shiga-toxin-producing-escherichia-coli-stec-to-livestock-sources-and-identification-of-source-specific-risk-factors-the-netherlands-2010-2014
#6
L Mughini-Gras, W van Pelt, M van der Voort, M Heck, I Friesema, E Franz
Shiga toxin-producing Escherichia coli (STEC) is a zoonotic pathogen of public health concern whose sources and transmission routes are difficult to trace. Using a combined source attribution and case-control analysis, we determined the relative contributions of four putative livestock sources (cattle, small ruminants, pigs, poultry) to human STEC infections and their associated dietary, animal contact, temporal and socio-econo-demographic risk factors in the Netherlands in 2010/2011-2014. Dutch source data were supplemented with those from other European countries with similar STEC epidemiology...
September 17, 2017: Zoonoses and Public Health
https://www.readbyqxmd.com/read/28914522/nanozyme-mediated-dual-immunoassay-integrated-with-smartphone-for-use-in-simultaneous-detection-of-pathogens
#7
Nan Cheng, Yang Song, Mohamed Maarouf Ali Zeinhom, Yu-Chung Chang, Lina Sheng, Haolin Li, Dan Du, Lei Li, Meijun Zhu, Yunbo Luo, Wentao Xu, Yuehe Lin
Nanozymes are an excellent class of optical reporters for the development of sensitive biosensors for widespread applications. In this study, platinum-palladium (Pt-Pd) nanoparticles were applied as signal amplifier in a dual-lateral flow immunoassays (LFIA) and integrated with a smartphone-based device for use in simultaneous detection of Salmonella Enteritidis and E. coli O157:H7. After optimization, the LODs were calculated to be ∼20 CFU/mL for Salmonella Enteritidis and ∼34 CFU/mL for E. coli O157:H7, respectively...
September 15, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28914098/the-antimicrobial-effects-of-three-phenolic-extracts-from-rosmarinus-officinalis-l-vitis-vinifera-l-and-polygonum-cuspidatum-l-on-food-pathogens
#8
Francesca Santomauro, Cristiana Sacco, Rosa Donato, Maria Bellumori, Marzia Innocenti, Nadia Mulinacci
In this study three phenolic extracts were examined, without volatile fraction, against common food pathogens. The samples, all suitable for food application, were from the leaves of Rosmarinus officinalis L., Vitis vinifera L., and the root of Polygonum cuspidatum L. The microorganisms tested were Escherichia coli O157:H7, Salmonella Enteritidis, Salmonella Typhi, Yersinia enterocolitica and Listeria monocytogenes, well-known as important food pathogens. The results demonstrated a microbicidal activity of all the tested compounds at different concentrations; the rosemary extract showed greater efficacy than the other compounds against the tested microorganisms...
September 15, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28911637/safety-of-frozen-liver-for-human-consumption
#9
Ghada A K Kirrella, Azza M M Deeb, Reda Mohamed Ibrahim Abdallah
The objective of this study was to ensure and evaluate the safety of imported frozen beef liver traded in supermarkets of Kafr El-Sheikh Governorate, Egypt, through detection of Salmonella typhimurium, Salmonella enteritidies, Escherichia coli O157:H7, antibiotic residues, and aflatoxin B1 residue. Fifty samples of imported frozen liver were randomly collected from different shops at Kafr El-Sheikh Governorate for isolation of S. typhimurium, S. enteritidies, and E. coli O157:H7. The results revealed that for both microorganisms 4% of the examined samples presumed to contain Salmonella and E...
July 2017: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28902868/discovery-of-numerous-novel-small-genes-in-the-intergenic-regions-of-the-escherichia-coli-o157-h7-sakai-genome
#10
Sarah M Hücker, Zachary Ardern, Tatyana Goldberg, Andrea Schafferhans, Michael Bernhofer, Gisle Vestergaard, Chase W Nelson, Michael Schloter, Burkhard Rost, Siegfried Scherer, Klaus Neuhaus
In the past, short protein-coding genes were often disregarded by genome annotation pipelines. Transcriptome sequencing (RNAseq) signals outside of annotated genes have usually been interpreted to indicate either ncRNA or pervasive transcription. Therefore, in addition to the transcriptome, the translatome (RIBOseq) of the enteric pathogen Escherichia coli O157:H7 strain Sakai was determined at two optimal growth conditions and a severe stress condition combining low temperature and high osmotic pressure. All intergenic open reading frames potentially encoding a protein of ≥ 30 amino acids were investigated with regard to coverage by transcription and translation signals and their translatability expressed by the ribosomal coverage value...
2017: PloS One
https://www.readbyqxmd.com/read/28888654/shiga-toxin-producing-escherichia-coli-stec-o22-h8-isolated-from-cattle-reduces-e-coli-o157-h7-adherence-in-vitro-and-in-vivo
#11
L Martorelli, A Albanese, D Vilte, R Cantet, A Bentancor, G Zolezzi, I Chinen, C Ibarra, M Rivas, E C Mercado, A Cataldi
PROBLEM ADDRESSED: Shiga toxin-producing Escherichia coli (STEC) are a group of bacteria responsible for food-associated diseases. Clinical features include a wide range of symptoms such as diarrhea, hemorrhagic colitis and the hemolytic uremic syndrome (HUS), a life-threatening condition. OBJECTIVE: Our group has observed that animals naturally colonized with STEC strains of unknown serotype were not efficiently colonized with E. coli O157:H7 after experimental infection...
September 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28888104/effects-of-ultraviolet-light-emitting-diodes-leds-on-microbial-and-enzyme-inactivation-of-apple-juice
#12
Merve Pelvan Akgün, Sevcan Ünlütürk
In this study, the effects of Ultraviolet light-emitting diodes (UV-LEDs) on the inactivation of E. coli K12 (ATCC 25253), an indicator organism of E. coli O157:H7, and polyphneoloxidase (PPO) in cloudy apple juice (CAJ) were investigated. The clear (AJ) and cloudy apple juice were exposed to UV rays for 40min by using a UV device composed of four UV-LEDs with peak emissions at 254 and 280nm and coupled emissions as follows: 254/365, 254/405, 280/365, 280/405 and 254/280/365/405nm. UV-LEDs at 254nm achieved 1...
August 15, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28871242/concurrent-detection-of-human-norovirus-and-bacterial-pathogens-in-water-samples-from-an-agricultural-region-in-central-california-coast
#13
Peng Tian, David Yang, Lei Shan, Dapeng Wang, Qianqian Li, Lisa Gorski, Bertram G Lee, Beatriz Quiñones, Michael B Cooley
Bacterial pathogens and human norovirus (HuNoV) are major cause for acute gastroenteritis caused by contaminated food and water. Public waterways can become contaminated from a variety of sources and flood after heavy rain events, leading to pathogen contamination of produce fields. We initiated a survey of several public watersheds in a major leafy green produce production region of the Central California Coast to determine the prevalence of HuNoV as well as bacterial pathogens. Moore swabs were used to collect environmental samples bi-monthly at over 30 sampling sites in the region...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28869356/a-plant-produced-candidate-subunit-vaccine-reduces-shedding-of-enterohemorrhagic-escherichia-coli-in-ruminants
#14
Sean Miletic, Martin Hünerberg, Angelo Kaldis, Jacqueline MacDonald, Antoine Leuthreau, Tim McAllister, Rima Menassa
Enterohemorrhagic Escherichia coli (EHEC) are commonly present in the gastrointestinal tract of cattle and cause serious infectious disease in humans. Immunizing cattle against EHEC is a promising strategy to decrease the risk of food contamination; however, veterinary vaccines against EHEC such as Econiche have not been widely adopted by the agricultural industry, and have been discontinued, prompting the need for more cost-effective EHEC vaccines. The objective of this project was to develop a platform to produce plant-made antigens for oral vaccination of ruminants against EHEC...
September 4, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28865862/rapid-and-simultaneous-quantification-of-viable-escherichia-coli-o157-h7-and-salmonella-spp-in-milk-through-multiplex-real-time-pcr
#15
Baoqing Zhou, Taobo Liang, Zhongxu Zhan, Rui Liu, Fan Li, Hengyi Xu
Escherichia coli O157:H7 and Salmonella spp. in milk are 2 common pathogens that cause foodborne diseases. An accurate, rapid, specific method has been developed for the simultaneous detection of viable E. coli O157:H7 and Salmonella spp. in milk. Two specific genes, namely, fliC from E. coli O157:H7 and invA from Salmonella spp., were selected to design primers and probes. A combined treatment containing sodium deoxycholate (SDO) and propidium monoazide (PMA) was applied to detect viable E. coli O157:H7 and Salmonella spp...
August 30, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28860238/genome-sequences-of-30-escherichia-coli-o157-h7-isolates-recovered-from-a-single-dairy-farm-and-its-associated-off-site-heifer-raising-facility
#16
Seon-Woo Kim, Jeffrey S Karns, Jo Ann S Van Kessel, Bradd J Haley
Cattle are the primary reservoir of Escherichia coli O157:H7, the most frequently isolated serotype of enterohemorrhagic E. coli infections among humans in North America. To evaluate the diversity of E. coli O157:H7 isolates within a single dairy herd, the genomes of 30 isolates collected over a 7-year period were sequenced.
August 31, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28855894/rapid-flow-cytometry-detection-of-a-single-viable-escherichia-coli-o157-h7-cell-in-raw-spinach-using-a-simplified-sample-preparation-technique
#17
Anna J Williams, Willie M Cooper, Shawn Ramsaroop, Pierre Alusta, Dan A Buzatu, Jon G Wilkes
Very low cell count detection of Escherichia coli O157:H7 in foods is critical, since an infective dose for this pathogen may be only 10 cells, and fewer still for vulnerable populations. A flow cytometer is able to detect and count individual cells of a target bacterium, in this case E. coli O157:H7. The challenge is to find the single cell in a complex matrix like raw spinach. To find that cell requires growing it as quickly as possible to a number sufficiently in excess of matrix background that identification is certain...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28854682/effect-of-rnase-e-deficiency-on-translocon-protein-synthesis-in-an-rnase-e-inducible-strain-of-enterohemorrhagic-escherichia-coli-o157-h7
#18
Patricia B Lodato, Thujitha Thuraisamy, Jamie Richards, Joel G Belasco
Enterohemorrhagic Escherichia coli (EHEC) is a food-borne pathogen that assembles a type III secretion system (T3SS) on its surface. The last portion of the T3SS, called the 'translocon', is composed of a filament and a pore complex that is inserted into the membrane of intestinal epithelial cells. The genes encoding the translocon (espADB) are part of the LEE4 operon. Their expression is regulated by a complex post-transcriptional mechanism that involves the processing of LEE4 mRNA by the essential endoribonuclease RNase E...
July 6, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28853628/inactivation-of-escherichia-coli-o157-h7-in-minute-steaks-cooked-under-selected-conditions
#19
Xianqin Yang, Julia Devos, Mark D Klassen
A national survey was conducted in Canada to determine consumer cooking practices for minute steaks (thin, mechanically tenderized beef cutlets). Results indicate that most Canadians prefer cooking minute steaks by pan frying and to a medium level of doneness. To identify safe cooking conditions, retail minute steaks (∼125 g), inoculated at three sites per steak with a five-strain cocktail of nontoxigenic Escherichia coli O157:H7 (6.1 log CFU per site), were cooked on a hot plate (200°C), mimicking a pan-frying scenario...
August 30, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28846460/evaluation-of-commercial-%C3%AE-agonists-dietary-protein-and-shade-on-fecal-shedding-of-escherichia-coli-o157-h7-from-feedlot-cattle
#20
James E Wells, Elaine D Berry, Minseok Kim, Steven D Shackelford, Kristin E Hales
Escherichia coli O157:H7 is a foodborne pathogen commonly associated with cattle feces. Diet, including dietary supplements such as β-agonists, may impact fecal shedding of this pathogen. A series of three experiments were conducted to determine if the β-agonists ractopamine hydrochloride (RAC) or zilpaterol hydrochloride (ZH) would impact the level or prevalence of fecal E. coli O157:H7 shedding. In Experiment 1, dietary RAC did not impact fecal shedding of E. coli O157:H7 based on the level or prevalence, but the addition of dietary soybean meal (SBM) in the study did reduce E...
August 28, 2017: Foodborne Pathogens and Disease
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