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E. Coli O157

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https://www.readbyqxmd.com/read/28435191/occurrence-of-multidrug-resistant-escherichia-coli-in-groundwater-of-brij-region-uttar-pradesh-and-its-public-health-implications
#1
Barkha Sharma, Parul, A K Verma, Udit Jain, Janaradan K Yadav, Ravneet Singh, Raghvendra Mishra
AIM: The study evaluates the microbial as well as physicochemical pollution of groundwater of Brij region of Uttar Pradesh, a major tourist destination in the country along with estimating the drug resistance evident in the isolated Escherichia coli. MATERIALS AND METHODS: A total of 60 samples of groundwater were collected from six different sites and assessed for physicochemical (pH, color, taste, turbidity, total dissolved solids [TDS], total hardness [TH], chlorides, fluorides, nitrates, and iron) and microbiological parameters (standard plate count [SPC], most probable number test [MPN], E...
March 2017: Veterinary World
https://www.readbyqxmd.com/read/28431274/distribution-of-escherichia-coli-o157-h7-in-ground-beef-assessing-the-clustering-intensity-for-an-industrial-scale-grinder-and-a-low-and-localized-initial-contamination
#2
Estelle Loukiadis, Clémence Bièche-Terrier, Catherine Malayrat, Franck Ferré, Philippe Cartier, Jean-Christophe Augustin
Undercooked ground beef is regularly implicated in food-borne outbreaks involving pathogenic Shiga toxin-producing Escherichia coli. The dispersion of bacteria during mixing processes is of major concern for quantitative microbiological risk assessment since clustering will influence the number of bacteria the consumers might get exposed to as well as the performance of sampling plans used to detect contaminated ground beef batches. In this study, batches of 25kg of ground beef were manufactured according to a process mimicking an industrial-scale grinding with three successive steps: primary grinding, mixing and final grinding...
March 18, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28427631/combined-effect-of-ultrasound-heat-and-pressure-on-escherichia-coli-o157-h7-polyphenol-oxidase-activity-and-anthocyanins-in-blueberry-vaccinium-corymbosum-juice
#3
Jinyan Zhu, Yuehua Wang, Xinghe Li, Bin Li, Suwen Liu, Nan Chang, Ding Jie, Chong Ning, Haiyan Gao, Xianjun Meng
The objective of this study was to evaluate the effect of different treatments-heat treatment (HT), sonication (SC), thermosonication (TS), manosonication (MS), manothermal (MT), and manothermosonication (MTS) on Escherichia coli O157:H7, polyphenol oxidase (PPO), and anthocyanin content in blueberry juice. First, samples were treated at different temperatures (30, 40, 50, 60, 70, and 80°C) and power intensities (280, 420, 560, and 700W) for 10min. Subsequently, samples were treated using combinations of power intensity and mild temperature for 10min...
July 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28425902/evidence-for-the-benefits-of-food-chain-interventions-on-e-coli-0157-h7-nm-prevalence-in-retail-ground-beef-and-human-disease-incidence-a-success-story
#4
Frank Pollari, Tanya Christidis, Katarina D M Pintar, Andrea Nesbitt, Jeff Farber, Marie-Claude Lavoie, Alex Gill, Penelope Kirsch, Roger P Johnson
OBJECTIVES: Human infection with Escherichia coli O157:H7/NM has historically been associated with consumption of undercooked ground beef. The purpose of this paper is to investigate the correlation of the decline in E. coli O157:H7/NM infections in Canada with the introduction of control efforts in ground beef by industry. METHODS: The human incidence of E. coli O157:H7/NM, prevalence in ground beef and interventions from 1996 to 2014 were analyzed. Pathogen prevalence data were obtained from federal government and industry surveillance and inspection/compliance programs...
April 20, 2017: Canadian Journal of Public Health. Revue Canadienne de Santé Publique
https://www.readbyqxmd.com/read/28421309/genetic-makeup-of-shiga-toxin-producing-escherichia-coli-in-relation-to-clinical-symptoms-and-duration-of-shedding-a-microarray-analysis-of-isolates-from-swedish-children
#5
A Matussek, C Jernberg, I-M Einemo, S Monecke, R Ehricht, I Engelmann, S Löfgren, S Mernelius
Shiga toxin (Stx)-producing Escherichia coli (STECs) cause non-bloody diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome, and are the primary cause of acute renal failure in children worldwide. This study investigated the correlation of genetic makeup of STEC strains as revealed by DNA microarray to clinical symptoms and the duration of STEC shedding. All STEC isolated (n = 96) from patients <10 years of age in Jönköping County, Sweden from 2003 to 2015 were included. Isolates were characterized by DNA microarray, including almost 280 genes...
April 19, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28414257/spiral-plating-method-to-quantify-the-six-major-non-o157-escherichia-coli-serogroups-in-cattle-feces
#6
Pragathi B Shridhar, Lance W Noll, Charley A Cull, Xiaorong Shi, Natalia Cernicchiaro, David G Renter, Jianfa Bai, T G Nagaraja
Cattle are a major reservoir of the six major Shiga toxin-producing non-O157 Escherichia coli (STEC) serogroups (O26, O45, O103, O111, O121, and O145) responsible for foodborne illnesses in humans. Besides prevalence in feces, the concentrations of STEC in cattle feces play a major role in their transmission dynamics. A subset of cattle, referred to as super shedders, shed E. coli O157 at high concentrations (≥4 log CFU/g of feces). It is not known whether a similar pattern of fecal shedding exists for non-O157...
April 17, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28412931/prevalence-of-escherichia-coli-o157-h7-in-beef-cattle-at-slaughter-and-beef-carcasses-at-retail-shops-in-ethiopia
#7
Rosa Abdissa, Woynshet Haile, Akafete Teklu Fite, Ashenafi Feyisa Beyi, Getahun E Agga, Bedaso Mammo Edao, Fanos Tadesse, Mesula Geloye Korsa, Takele Beyene, Tariku Jibat Beyene, Lieven De Zutter, Eric Cox, Bruno Maria Goddeeris
BACKGROUND: There is paucity of information regarding the epidemiology of Escherichia coli O157: H7 in developing countries. In this study, we investigated the occurrence of E. coli O157: H7 associated with beef cattle at processing plants and at retail shops in Ethiopia. METHODS: Various samples were collected from beef cattle at slaughter/processing plants, carcass at retail shops and humans at health centers. E. coli O157: H7 was isolated, identified and characterized for antimicrobial resistance, using standard microbiological methods...
April 17, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28406080/genome-amplification-and-promoter-mutation-expand-the-range-of-csgd-dependent-biofilm-responses-in-an-stec-population
#8
Gaylen A Uhlich, Chin-Yi Chen, Bryan J Cottrell, Elisa Andreozzi, Peter L Irwin, Ly-Huong Nguyen
Expression of the major biofilm components of E. coli, curli fimbriae and cellulose, requires the CsgD transcription factor. A complex regulatory network allows environmental control of csgD transcription and biofilm formation. However, most clinical serotype O157 : H7 strains contain prophage insertions in the csgD regulator, mlrA, or mutations in other regulators that restrict csgD expression. These barriers can be circumvented by certain compensating mutations that restore higher csgD expression. One mechanism is via csgD promoter mutations that switch sigma factor utilization...
April 13, 2017: Microbiology
https://www.readbyqxmd.com/read/28402187/detection-and-isolation-of-the-top-seven-shiga-toxin-producing-escherichia-coli-in-ground-beef-comparison-of-rapidfinder-kits-to-the-u-s-department-of-agriculture-microbiology-laboratory-guidebook-method
#9
Pina M Fratamico, Lori K Bagi, Aisha Abdul-Wakeel
Shiga toxin-producing Escherichia coli (STEC) O157:H7 and serogroups O26, O45, O103, O111, O121, and O145 are often referred to as the "top seven" STEC, and these have been declared to be adulterants in beef by the U.S. Department of Agriculture (USDA) Food Safety and Inspection Service (FSIS). The aim of this work was to compare the methods described in the USDA FSIS Microbiology Laboratory Guidebook (MLG) to a two-stage Applied Biosystems RapidFinder STEC real-time PCR method to test for the top seven STEC in raw ground beef...
April 12, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28400770/antibacterial-action-of-jineol-isolated-from-scolopendra-subspinipes-mutilans-against-selected-foodborne-pathogens
#10
Vivek K Bajpai, Shruti Shukla, Woon K Paek, Jeongheui Lim, Pradeep Kumar, MinKyun Na
This study was undertaken to assess the antibacterial potential of 3,8-dihydroxyquinoline (jineol) isolated from Scolopendra subspinipes mutilans against selected foodborne pathogens Escherichia coli O157:H7 and Staphylococcus aureus KCTC-1621. Jineol at the tested concentration (50 μL; corresponding to 250 μg/disk) exhibited significant antibacterial effects as a diameter of inhibition zones (11.6-13.6 mm), along with minimum inhibitory concentration (MIC) and minimum bactericidal concentration values found in the range of (62...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28400023/effect-of-salt-addition-on-acid-resistance-response-of-escherichia-coli-o157-h7-against-acetic-acid
#11
Young-Min Bae, Sun-Young Lee
A combination of salt and acid is commonly used in the production of many foods, such as pickles and fermented foods. However, in our previous studies, addition of salt significantly reduced the inhibitory effect of acetic acid against E. coli O157:H7 in laboratory media and pickled cucumbers. Therefore, this study was conducted to determine the effect of salt addition on the acid resistance (AR) response of E. coli O157:H7 after treatment with acetic acid. The combined effect of acetic acid and salt showed different results depending on media tested...
August 2017: Food Microbiology
https://www.readbyqxmd.com/read/28400011/use-of-enhanced-nisin-derivatives-in-combination-with-food-grade-oils-or-citric-acid-to-control-cronobacter-sakazakii-and-escherichia-coli-o157-h7
#12
Alicia Campion, Ruth Morrissey, Des Field, Paul D Cotter, Colin Hill, R Paul Ross
Cronobacter sakazakii and Escherichia coli O157:H7 are well known food-borne pathogens that can cause severe disease. The identification of new alternatives to heating to control these pathogens in foods, while reducing the impact on organoleptic properties and nutritional value, is highly desirable. In this study, nisin and its bioengineered variants, nisin V and nisin S29A, are used alone, or in combination with plant essential oils (thymol, carvacrol and trans-cinnamaldehyde) or citric acid, with a view to controlling C...
August 2017: Food Microbiology
https://www.readbyqxmd.com/read/28399992/microbial-quality-of-irrigation-water-used-in-leafy-green-production-in-southern-brazil-and-its-relationship-with-produce-safety
#13
Luana Tombini Decol, Letícia Sopeña Casarin, Claudia Titze Hessel, Ana Carolina Fösch Batista, Ana Allende, Eduardo César Tondo
Irrigation water has been recognized as an important microbial risk factor for fruits and vegetables in many production areas, but there is still a lack of information about how the microbiological quality of different irrigation water sources and climatic conditions influence the safety of vegetables produced in Brazil. This study evaluated the distribution of generic E. coli and the prevalence of E. coli O157:H7 in two different water sources (ponds and streams bordering farmlands and urban areas) used for irrigation and on commercially produced lettuces in Southern Brazil...
August 2017: Food Microbiology
https://www.readbyqxmd.com/read/28399991/in-package-inhibition-of-e-%C3%A2-coli-o157-h7-on-bulk-romaine-lettuce-using-cold-plasma
#14
Sea C Min, Si Hyeon Roh, Brendan A Niemira, Glenn Boyd, Joseph E Sites, Joseph Uknalis, Xuetong Fan
Dielectric barrier discharge atmospheric cold plasma (DACP) treatment was evaluated for the inactivation of Escherichia coli O157:H7, surface morphology, color, carbon dioxide generation, and weight loss of bulk Romaine lettuce in a commercial plastic clamshell container. The lettuce samples were packed in a model bulk packaging configuration (three rows with either 1, 3, 5, or 7 layers) in the container and treated by DACP (42.6 kV, 10 min). DACP treatment reduced the number of E. coli O157:H7 in the leaf samples in the 1-, 3-, and 5-layer configurations by 0...
August 2017: Food Microbiology
https://www.readbyqxmd.com/read/28399273/house-flies-in-the-confined-cattle-environment-carry-non-o157-shiga-toxin-producing-escherichia-coli
#15
R Puri-Giri, A Ghosh, J L Thomson, L Zurek
Cattle manure is one of the primary larval developmental habitats of house flies, Musca domestica (L.). Cattle serve as asymptomatic reservoirs of Shiga toxin-producing Escherichia coli (STEC), and bacteria are released into the environment in cattle feces. The USDA-FSIS declared seven STEC serogroups (O157, O26, O45, O103, O145, O121, and O111) as adulterants in beef products. In addition, the serogroup O104 was a culprit of a large outbreak in Germany in 2011. Our study aimed to assess the prevalence of seven non-O157 STEC (O26, O45, O145, O103, O121, O111, and O104) serogroups in adult house flies...
March 3, 2017: Journal of Medical Entomology
https://www.readbyqxmd.com/read/28398867/antimicrobial-efficacy-of-cinnamaldehyde-against-escherichia-coli-o157-h7-and-salmonella-enterica-in-carrot-juice-and-mixed-berry-juice-held-at-4%C3%A2-c-and-12%C3%A2-c
#16
David Manu, Aubrey F Mendonca, Aura Daraba, James S Dickson, Joseph Sebranek, Angela Shaw, Fei Wang, Shecoya White
The effectiveness of cinnamaldehyde for inactivating Salmonella enterica and Escherichia coli O157:H7 in carrot juice (CRJ) and mixed berry juice (MBJ) was investigated. Brain heart infusion broth (BHI), CRJ, and MBJ, with concentrations of added cinnamaldehyde ranging from 0.15 to 1.5 μL/mL, 0.25 to 2.0 μL/mL, and 0.25 to 1.5 μL/mL, respectively, were each inoculated with a 5-strain mixture of Salmonella enterica or Escherichia coli O157:H7 to give an initial viable count of 5.07 log10 colony-forming units/mL...
February 21, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28397273/internalisation-of-escherichia-coli-o157-h7-gfp-in-rocket-and-swiss-chard-baby-leaves-as-affected-by-abiotic-and-biotic-damage
#17
Rahel Hartmann, Andreas Fricke, Hartmut Stützel, Suzan Mansourian, Teun Dekker, Walter Wohanka, Beatrix Alsanius
Internalisation of human pathogens in edible parts of vegetables eaten raw is a major concern, since once internalised they are protected from sanitising treatments. In this study, we examined invasion of gfp-labelled E. coli O157:H7 into intact and biotically (infection with Xanthomonas campestris/Pseudomonas syringae) and abiotically (grating with silicon carbide) damaged leaves of wild rocket (Diplotaxis tenuifolia) and Swiss chard (Beta vulgaris subsp. cicla) using laser scanning confocal microscopy. Bacterial cells were found in internal locations of the tissue, irrespective of tissue health status...
April 11, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28391475/a-study-on-prevalence-of-microbial-contamination-on-the-surface-of-raw-salad-vegetables
#18
Sujeet Kumar Mritunjay, Vipin Kumar
The present work evaluates the microbiological quality of raw salad vegetables (RSV) consumed in Dhanbad city, India. A total of 480 samples of 8 different raw salad vegetables from local market were examined for overall microbial quality in terms of aerobic mesophilic, psychrotrophic counts, yeast, mould and total coliform levels. E. coli O157:H7, Listeria monocytogenes and Salmonella sp. were detected by real-time polymerase chain reaction (qPCR) subsequent to isolation. Results showed that all the samples were found positive for total coliform; however, E...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28386444/fabrication-and-characterization-of-electrospun-poly-e-caprolactone-fibrous-membrane-with-antibacterial-functionality
#19
Idris Cerkez, Ayse Sezer, Sukhwinder K Bhullar
This research study is mainly targeted on fabrication and characterization of antibacterial poly(e-caprolactone) (PCL) based fibrous membrane containing silver chloride particles. Micro/nano fibres were produced by electrospinning and characterized with TGA, DSC, SEM and mechanical analysis. It was found that addition of silver particles slightly reduced onset of thermal degradation and increased crystallization temperature of neat PCL. Silver-loaded samples exhibited higher tensile stress and lower strain revealing that the particles behaved as reinforcing agent...
February 2017: Royal Society Open Science
https://www.readbyqxmd.com/read/28382165/an-electrochemical-strategy-using-multifunctional-nanoconjugates-for-efficient-simultaneous-detection-of-escherichia-coli-o157-h7-and-vibrio-cholerae-o1
#20
Yan Li, Ya Xiong, Lichao Fang, Lili Jiang, Hui Huang, Jun Deng, Wenbin Liang, Junsong Zheng
The rapid and accurate quantification of the pathogenic bacteria is extremely critical to decrease the bacterial infections in all areas related to health and safety. We have developed an electrochemical strategy for simultaneous ultrasensitive detection of E. coli O157:H7 and Vibrio cholerae O1. This approach was based on the specific immune recognition of different pathogenic bacteria by multifunctional nanoconjugates and subsequent signal amplification. By employing the proposed biosensor, the concentrations of these pathogenic bacteria could be established on a single interface in a single run with improved sensitivity and accuracy...
2017: Theranostics
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