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E. Coli O157

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https://www.readbyqxmd.com/read/29337344/microbiological-quality-and-safety-of-fresh-fruits-and-vegetables-at-retail-levels-in-korea
#1
Charles Nkufi Tango, Shuai Wei, Imran Khan, Mohammad Shakhawat Hussain, Paul-François Ngnitcho Kounkeu, Joong-Hyun Park, Se-Hun Kim, Deog Hwan Oh
The aim of this study was to evaluate the microbiological quality and safety of fresh produce at retail level in Korea in order to periodically update information and establish available risks associated with consumption of fresh fruits and vegetables. The samples from different markets located in 3 provinces of South Korea were collected. The protocol in the Korean Food Standards Codex was applied and generic Escherichia coli, coliforms, aerobic mesophilic bacteria (AMB), and yeast and mold (YM) in 360 packaged and unpackaged fresh fruits and vegetables were analyzed...
January 16, 2018: Journal of Food Science
https://www.readbyqxmd.com/read/29328809/methods-for-the-control-of-foodborne-pathogens-in-low-moisture-foods
#2
Alma Fernanda Sánchez-Maldonado, Alvin Lee, Jeffrey M Farber
Low-moisture foods (LMFs) have been defined as those food products with a water activity (aw) less than 0.85 and are generally considered less susceptible to microbial spoilage and the growth of foodborne pathogens. However, in recent years, outbreaks linked to LMFs have increased, with Salmonella spp., Bacillus cereus, Cronobacter sakazakii, Clostridium spp., Escherichia coli O157:H7, non-O157 E. coli, and Staphylococcus aureus being the principal pathogens involved. Because of the new concerns raised as a result of recent outbreaks, new approaches need to be developed to control foodborne pathogens in LMFs...
January 12, 2018: Annual Review of Food Science and Technology
https://www.readbyqxmd.com/read/29326391/zoonotic-bacteria-and-parasites-found-in-raw-meat-based-diets-for-cats-and-dogs
#3
Freek P J van Bree, Gertie C A M Bokken, Robin Mineur, Frits Franssen, Marieke Opsteegh, Joke W B van der Giessen, Len J A Lipman, Paul A M Overgaauw
Feeding raw meat-based diets (RMBDs) to companion animals has become increasingly popular. Since these diets may be contaminated with bacteria and parasites, they may pose a risk to both animal and human health. The purpose of this study was to test for the presence of zoonotic bacterial and parasitic pathogens in Dutch commercial RMBDs. We analysed 35 commercial frozen RMBDs from eight different brands. Escherichia coli serotype O157:H7 was isolated from eight products (23 per cent) and extended-spectrum beta-lactamases-producing E coli was found in 28 products (80 per cent)...
January 13, 2018: Veterinary Record
https://www.readbyqxmd.com/read/29320233/antibacterial-activities-of-hibiscus-sabdariffa-extracts-and-chemical-sanitizers-directly-on-green-leaves-contaminated-with-foodborne-pathogens
#4
Carlos A Gómez-Aldapa, Esmeralda Rangel-Vargas, Ma Refugio Torres-Vitela, Angélica Villarruel-López, Otilio A Acevedo-Sandoval, Alberto J Gordillo-Martínez, Angélica Godínez-Oviedo, Javier Castro-Rosas
Leafy greens have been associated with foodborne disease outbreaks in different countries. To decrease microbial contamination of leafy greens, chemical agents are commonly used; however, a number of studies have shown these agents to have limited antimicrobial effect against pathogenic bacteria on vegetables. The objective of this study was to compare the antibacterial effect of Hibiscus sabdariffa calyx extracts (water, methanol, acetone, and ethyl acetate), sodium hypochlorite, acetic acid, and colloidal silver against foodborne bacteria on leafy greens...
January 10, 2018: Journal of Food Protection
https://www.readbyqxmd.com/read/29315715/a-mathematical-model-for-pathogen-cross-contamination-dynamics-during-the-postharvest-processing-of-leafy-greens
#5
Amir Mokhtari, David Oryang, Yuhuan Chen, Regis Pouillot, Jane Van Doren
We developed a probabilistic mathematical model for the postharvest processing of leafy greens focusing on Escherichia coli O157:H7 contamination of fresh-cut romaine lettuce as the case study. Our model can (i) support the investigation of cross-contamination scenarios, and (ii) evaluate and compare different risk mitigation options. We used an agent-based modeling framework to predict the pathogen prevalence and levels in bags of fresh-cut lettuce and quantify spread of E. coli O157:H7 from contaminated lettuce to surface areas of processing equipment...
January 8, 2018: Risk Analysis: An Official Publication of the Society for Risk Analysis
https://www.readbyqxmd.com/read/29307571/mixing-of-shiga-toxin-producing-and-enteropathogenic-escherichia-coli-in-a-wastewater-treatment-plant-receiving-city-and-slaughterhouse-wastewater
#6
Delphine Bibbal, Maryse Michèle Um, Alpha Amadou Diallo, Monique Kérourédan, Véronique Dupouy, Pierre-Louis Toutain, Alain Bousquet-Mélou, Eric Oswald, Hubert Brugère
Wastewater of human and animal may contain Shiga toxin-producing (STEC) and enteropathogenic (EPEC) Escherichia coli. We evaluated the prevalence of such strains in a wastewater treatment plant (WWTP) receiving both city and slaughterhouse wastewater. PCR screenings were performed on 12,248 E. coli isolates. The prevalence of STEC in city wastewater, slaughterhouse wastewater and treated effluent was 0.22%, 0.07% and 0.22%, respectively. The prevalence of EPEC at the same sampling sites was 0.63%, 0.90% and 0...
December 29, 2017: International Journal of Hygiene and Environmental Health
https://www.readbyqxmd.com/read/29306732/sequential-effect-of-phages-and-cold-nitrogen-plasma-against-escherichia-coli-o157-h7-biofilms-on-different-vegetables
#7
Haiying Cui, Mei Bai, Lu Yuan, Duraiarasan Surendhiran, Lin Lin
Escherichia coli O157:H7 (E. coli O157:H7) is one of the most common pathogens in fresh vegetables and fruits, and most of the diseases produced by E. coli O157:H7 are associated with biofilms. Cold nitrogen plasma (CNP) is a cold sterilization technique which has no residue. However to completely eliminate the biofilm on the surface of vegetables the processing power and time of CNP have to be enhanced, which will impact on the quality of fruits and vegetables. Thus the sequential treatment of CNP and phage techniques was engineered in this study...
January 3, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29300761/investigation-of-prevalence-of-free-shiga-toxin-producing-escherichia-coli-stec-specific-bacteriophages-and-its-correlation-with-stec-bacterial-hosts-in-a-produce-growing-area-in-salinas-california
#8
Yen-Te Liao, Irwin A Quintela, Kimberly Nguyen, Alexandra Salvador, Michael B Cooley, Vivian C H Wu
Shiga toxin-producing E. coli (STEC) causes approximately 265,000 illnesses and 3,600 hospitalizations annually and is highly associated with animal contamination due to the natural reservoir of ruminant gastrointestinal tracts. Free STEC-specific bacteriophages against STEC strains are also commonly isolated from fecal-contaminated environment. Previous studies have evaluated the correlation between the prevalence of STEC-specific bacteriophages and STEC strains to improve animal-associated environment. However, the similar information regarding free STEC-specific bacteriophages prevalence in produce growing area is lacking...
2018: PloS One
https://www.readbyqxmd.com/read/29280543/associations-between-escherichia-coli-o157-shedding-and-the-fecal-microbiota-of-dairy-cows
#9
Chloe Stenkamp-Strahm, Craig McConnel, Sheryl Magzamen, Zaid Abdo, Stephen Reynolds
AIMS: Dairy cattle shed pathogenic E. coli O157 (O157) in feces, playing a role in human exposure. We aimed to measure fecal microbial communities in early lactation dairy cattle, and model outcomes with O157 shedding metrics. METHODS AND RESULTS: Daily fecal samples were collected from 40 cattle on two Colorado dairies for five consecutive days, and characterized for O157. 16S rRNA gene sequencing was used to measure sample-level microbial communities. Alpha diversity metrics were associated with O157 outcomes via regression modeling, adjusting for confounders...
December 27, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29280319/importance-of-the-rpoe-regulon-in-maintaining-the-lipid-bilayer-during-antimicrobial-treatment-with-the-polycationic-agent-chlorhexidine
#10
Sinisa Vidovic, Prabhakara Medihala, James J Dynes, Prasad Daida, Vladimir Vujanovic, Adam P Hitchcock, Deeksha Shetty, Haixia Zhang, David R Brown, John R Lawrence, Darren R Korber
The emergence of multidrug resistance has reached alarming levels. To solve this growing problem, discovery of novel cellular targets or pathways important for antimicrobial resistance is urgently needed. In this study, we explored how the alternative sigma factor, RpoE, protects Escherichia coli O157 against the toxic effects of the polycationic antimicrobial agent, chlorhexidine (CHX). Susceptibility of this organism to CHX was found to directly correlate to the growth rate, with the faster-replicating wild-type being more susceptible to CHX than its more slowly-replicating ΔrpoE O157 mutant...
December 26, 2017: Proteomics
https://www.readbyqxmd.com/read/29278379/a-comparison-study-of-quality-attributes-of-ground-beef-and-veal-patties-and-thermal-inactivation-of-escherichia-coli-o157-h7-after-double-pan-broiling-under-dynamic-conditions
#11
KaWang Li, Amanda Gipe McKeith, Cangliang Shen, Russell McKeith
This study compared the quality variation and thermal inactivation of Escherichia coli O157:H7 in non-intact beef and veal. Coarse ground beef and veal patties (2.1 cm thick, 12.4 cm diameter, 180 g) inoculated with E. coli O157:H7, aerobically stored before double pan-broiling for 0-360 s without rest or to 55, 62.5, 71.1, and 76 °C (internal temperature) with 0.5- or 3.5-min rest. Microbial population and qualities including color, cooking losses, pH, water activity, fat, and moisture content, were tested...
December 26, 2017: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/29276663/detection-of-viable-but-non-culturable-escherichia-coli-o157-h7-by-pcr-in-combination-with-propidium-monoazide
#12
Junliang Zhong, Xihong Zhao
The aim of this study was to evaluate the applicability of the conventional PCR detection method combined with propidium monoazide (PMA) treatment for the detection of viable but non-culturable (VBNC) state Escherichia coli O157:H7 in ground beef meatballs. Under low temperature, E. coli O157:H7 cells were induced into the VBNC state in ground beef meatballs at - 20 °C after 152 days. The optimal PMA concentration of 5 µg/mL was obtained in beef meatball samples, which could completely inhibit the DNA amplification on dead cells (106 cells/mL) but with no inhibition on viable cells...
January 2018: 3 Biotech
https://www.readbyqxmd.com/read/29274960/bacterial-diversity-and-composition-of-alfalfa-silage-as-analyzed-by-illumina-miseq-sequencing-effects-of-escherichia-coli-o157-h7-and-silage-additives
#13
I M Ogunade, Y Jiang, A A Pech Cervantes, D H Kim, A S Oliveira, D Vyas, Z G Weinberg, K C Jeong, A T Adesogan
The first objective of this study was to examine effects of adding Escherichia coli O157:H7 with or without chemical or microbial additives on the bacterial diversity and composition of alfalfa silage. The second objective was to examine associations between the relative abundance of known and unknown bacterial species and indices of silage fermentation quality. Alfalfa forage was harvested at 54% dry matter, chopped to a theoretical length of cut of 19 mm, and ensiled in quadruplicate in laboratory silos for 100 d after the following treatments were applied: (1) distilled water (control); (2) 1 × 105 cfu/g of E...
December 20, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/29260688/geogenomic-segregation-and-temporal-trends-of-human-pathogenic-escherichia-coli-o157-h7-washington-usa-2005-20141
#14
Gillian A M Tarr, Smriti Shringi, Amanda I Phipps, Thomas E Besser, Jonathan Mayer, Hanna N Oltean, Jon Wakefield, Phillip I Tarr, Peter Rabinowitz
The often-noted and persistent increased incidence of Escherichia coli O157:H7 infections in rural areas is not well understood. We used a cohort of E. coli O157:H7 cases reported in Washington, USA, during 2005-2014, along with phylogenomic characterization of the infecting isolates, to identify geographic segregation of and temporal trends in specific phylogenetic lineages of E. coli O157:H7. Kernel estimation and generalized additive models demonstrated that pathogen lineages were spatially segregated during the period of analysis and identified a focus of segregation spanning multiple, predominantly rural, counties for each of the main clinical lineages, Ib, IIa, and IIb...
January 2018: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/29242148/whole-bacterium-selex-of-dna-aptamers-for-rapid-detection-of-e-coli-o157-h7-using-a-qcm-sensor
#15
Xiaofan Yu, Fang Chen, Ronghui Wang, Yanbin Li
The rapid detection of foodborne pathogens is critical to ensure food safety. The objective of this study is to select aptamers specifically bound to Escherichia coli O157:H7 using the whole-bacterium SELEX (Systematic Evolution of Ligands by Exponential Enrichment) and apply the selected aptamer to a QCM (quartz crystal microbalance) sensor for rapid and sensitive detection of target bacteria. A total of 19 rounds of selection against live E. coli O157:H7 and 6 rounds of counter selection against a mixture of Staphylococcus aureus, Listeria monocytogenes, and Salmonella Typhimurium, were performed...
December 12, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/29236304/enterovirus-detection-in-patients-with-acute-gastroenteritis-in-switzerland
#16
Rainer Gosert, Ulrich Heininger, Hans H Hirsch
Acute gastroenteritis (GE) has a major impact on morbidity and mortality worldwide, yet comprehensive data regarding infectious agents including enteroviruses are scarce. We hypothesized that enteroviruses constitute a significant cause of acute GE. We analyzed 677 stool samples from 504 patients, which had been submitted for suspected infectious GE. 0.2 ml of stool suspension was extracted using the Abbott m2000 sp robot and analysed by multiplex nucleic acid testing (NAT) using the Luminex xTAG gastrointestinal pathogen panel (GPP) as well as by specific NATs detecting enteroviruses and polioviruses...
December 13, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/29235272/real-time-detection-of-antibiotic-activity-by-measuring-nanometer-scale-bacterial-deformation
#17
Rafael Iriya, Karan Syal, Wenwen Jing, Manni Mo, Hui Yu, Shelley E Haydel, Shaopeng Wang, Nongjian Tao
Diagnosing antibiotic-resistant bacteria currently requires sensitive detection of phenotypic changes associated with antibiotic action on bacteria. Here, we present an optical imaging-based approach to quantify bacterial membrane deformation as a phenotypic feature in real-time with a nanometer scale (∼9  nm) detection limit. Using this approach, we found two types of antibiotic-induced membrane deformations in different bacterial strains: polymyxin B induced relatively uniform spatial deformation of Escherichia coli O157:H7 cells leading to change in cellular volume and ampicillin-induced localized spatial deformation leading to the formation of bulges or protrusions on uropathogenic E...
December 2017: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/29233680/new-fpg-probe-chemistry-for-direct-detection-of-recombinase-polymerase-amplification-on-lateral-flow-strips
#18
Michael L Powell, Frank R Bowler, Aurore J Martinez, Catherine J Greenwood, Niall Armes, Olaf Piepenburg
Rapid, cost-effective and sensitive detection of nucleic acids has the ability to improve upon current practices employed for pathogen detection in diagnosis of infectious disease and food testing. Furthermore, if assay complexity can be reduced, nucleic acid amplification tests could be deployed in resource-limited and home use scenarios. In this study, we developed a novel Fpg (Formamidopyrimidine DNA glycosylase) probe chemistry, which allows lateral flow detection of amplification in undiluted recombinase polymerase amplification (RPA) reactions...
December 9, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/29232632/inactivation-of-escherichia-coli-o157-h7-and-salmonella-deposited-on-gloves-in-a-liquid-state-and-subjected-to-drying-conditions
#19
Marilyn C Erickson, Jye-Yin Liao, Cathy C Webb, Mussie Y Habteselassie, Jennifer L Cannon
Gloves are worn by workers harvesting ready-to-eat produce as a deterrent for contaminating the produce with enteric pathogens that may reside on their hands. As fields are not sterile environments, the probability for gloves to become contaminated still exists and therefore it is critical to understand the conditions that affect the survival of pathogens on gloves. Both Escherichia coli O157:H7 and Salmonella deposited on glove surfaces in a liquid state survived longer when the pathogen had been suspended in lettuce sap than when suspended in water...
November 29, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29230401/shiga-toxin-producing-escherichia-coli-o157-shedding-dynamics-in-an-australian-beef-herd
#20
Christina Ahlstrom, Petra Muellner, Geraldine Lammers, Meghan Jones, Sophie Octavia, Ruiting Lan, Jane Heller
Shiga toxin-producing Escherichia coli (STEC) O157 is an important foodborne pathogen that can be transmitted to humans both directly and indirectly from the feces of beef cattle, its primary reservoir. Numerous studies have investigated the shedding dynamics of E. coli O157 by beef cattle; however, the spatiotemporal trends of shedding are still not well understood. Molecular tools can increase the resolution through the use of strain typing to explore transmission dynamics within and between herds and identify strain-specific characteristics that may influence pathogenicity and spread...
2017: Frontiers in Veterinary Science
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