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E. Coli O157

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https://www.readbyqxmd.com/read/28741927/rapid-antibiotic-susceptibility-testing-of-uropathogenic-e-coli-by-tracking-sub-micron-scale-motion-of-single-bacterial-cells
#1
Karan Syal, Simon Shen, Yunze Yang, Shaopeng Wang, Shelley E Haydel, Nongjian Tao
To combat antibiotic resistance, a rapid antibiotic susceptibility testing (AST) technology that can identify resistant infections at disease onset is required. Current clinical AST technologies take 1-3 days, which is often too slow for accurate treatment. Here we demonstrate a rapid AST method by tracking sub-µm scale bacterial motion with an optical imaging and tracking technique. We apply the method to clinically relevant bacterial pathogens, Escherichia coli O157: H7 and uropathogenic E. coli (UPEC) loosely tethered to a glass surface...
July 25, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28736954/the-impact-of-the-bovine-fecal-microbiome-on-escherichia-coli-o157-h7-prevalence-and-enumeration-in-naturally-infected-cattle
#2
M Kim, L A Kuehn, J L Bono, E D Berry, N Kalchayanand, H C Freetly, A K Benson, J E Wells
AIMS: The objective of this study was to determine if the fecal microbiome has an association with E. coli O157:H7 prevalence and enumeration. METHODS AND RESULTS: Pyrosequencing analysis of fecal microbiome was performed from feedlot cattle fed one of three diets: i) 94 heifers fed low concentrate (LC) diet, ii) 142 steers fed moderate concentrate (MC) diet, and iii) 132 steers fed high concentrate (HC) diet. A total of 322,585 OTUs were calculated from 2,411,122 high-quality sequences obtained from 368 fecal samples...
July 23, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28735241/a-new-integrated-approach-to-analyze-bulk-tank-milk-and-raw-milk-filters-for-the-presence-of-the-e-coli-serogroups-frequently-associated-with-vtec-status
#3
Francesca Albonico, Claudia Gusmara, Theo Gugliotta, Monica Loiacono, Michele Mortarino, Alfonso Zecconi
We optimized a combination of microbiological and molecular methods to quickly identify the presence of the O157 and the six non-O157 serogroups (O26, O45, O103, O111, O121 and O145) most frequently associated with VTEC status, at herd level. The lower detection limit of this methodology is 10(1)CFU/ml for each of the serogroups tested. We tested 67 bulk tank milk (BTM) and raw milk filters (RMF) derived from dairy herds located in Lombardy and Trentino Alto Adige. We identified 3 positive samples and 20 positive samples out of 67 respectively in the BTM and RMF...
July 17, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28732694/development-and-evaluation-of-latex-agglutination-tests-for-the-detection-of-human-antibodies-to-the-lipopolysaccharides-of-verocytotoxin-producing-escherichia-coli-vtec-serogroups-o157-and-non-o157
#4
Waldemar Rastawicki, Anna Chróst, Kornelia Gielarowiec
Latex agglutination tests (LAT) were developed and evaluated for the rapid detection of LPS antibodies against E. coli serogroup O157, O26, O104, O111 and O145. The latex tests have been proved to be sensitive, fast, easy-to-perform and cost-efficient tools for the screening serodiagnosis of VTEC infections causing haemolytic-uraemic syndrome.
July 18, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28728309/escherichia-coli-outer-membrane-protein-f-ompf-an-immunogenic-protein-induces-cross-reactive-antibodies-against-escherichia-coli-and-shigella
#5
Xiao Wang, Da Teng, Qingfeng Guan, Ruoyu Mao, Ya Hao, Xiumin Wang, Junhu Yao, Jianhua Wang
Diarrhea caused by pathogenic Escherichia coli (E. coli) is one of the most serious infectious diseases in humans and animals. Due to antibiotics resistance and the lack of efficient vaccine, more attention should be paid to find potential versatile vaccine candidates to prevent diseases. In this study, the sequence homology analysis indicated that OmpF from E. coli CVCC 1515 shares a high identity (90-100%) with about half of the E. coli (46.7%) and Shigella (52.8%) strains. Then the recombinant OmpF was supposed to be developed as a versatile vaccine to prevent E...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28726488/use-of-an-electrostatic-spraying-system-or-the-sprayed-lethality-in-container-method-to-deliver-antimicrobial-agents-onto-the-surface-of-beef-subprimals-to-control-shiga-toxin-producing-escherichia-coli
#6
J Max Stella, John B Luchansky, Kelsey Miller, Bradley A Shoyer, Laura E Shane, Lianna McGeary, Manuela Osoria, Laura J Stahler, Nicholas J Sevart, Randall K Phebus, Harshavardhan Thippareddi, Anna C S Porto-Fett
The efficacy of an electrostatic spraying system (ESS) and/or the sprayed lethality in container (SLIC) method to deliver antimicrobial agents onto the surface of beef subprimals to reduce levels of Shiga toxin-producing Escherichia coli (STEC) was evaluated. Beef subprimals were surface inoculated (lean side; ca. 5.8 log CFU per subprimal) with 2 mL of an eight-strain cocktail comprising single strains of rifampin-resistant (100 μg/mL) STEC (O26:H11, O45:H2, O103:H2, O104:H4, O111:H(-), O121:H19, O145:NM, and O157:H7)...
July 20, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28723213/dual-functional-core-shell-fluorescent-ag2s-carbon-nanostructure-for-selective-assay-of-e-coli-o157-h7-and-bactericidal-treatment
#7
Ning Wang, Xing Wei, An-Qi Zheng, Ting Yang, Ming-Li Chen, Jian-Hua Wang
A dual functional fluorescent core-shell Ag2S@Carbon nanostructure is prepared by a hydrothermally assisted multi-amino synthesis approach with folic acid (FA), polyethylenimine (PEI), and mannoses (Mans) as carbon and nitrogen sources (FA-PEI-Mans-Ag2S nanocomposite shortly as Ag2S@C). The nanostructure exhibits strong fluorescent emission at λex/λem = 340/450 nm with a quantum yield of 12.57 ± 0.52%. Ag2S@C is bound to E. coli O157:H7 via strong interaction with the Mans moiety in Ag2S@C with FimH proteins on the fimbriae tip in E...
March 24, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28711240/characterization-and-bioactivities-of-the-exopolysaccharide-from-a-probiotic-strain-of-lactobacillus-plantarum-wlpl04
#8
Zhengqi Liu, Zhihong Zhang, Liang Qiu, Fen Zhang, Xiongpeng Xu, Hua Wei, Xueying Tao
Exopolysaccharide (EPS) was extracted and purified from Lactobacillus plantarum WLPL04, which has been confirmed previously as a potential probiotic for its antagonistic and immune-modulating activity. It has a molecular weight of 6.61 × 10(4) Da, consisting of xylose, glucose, and galactose in an approximate molar ratio of 3.4: 1.8: 1. Microstructural studies demonstrated that the EPS appeared as a smooth sheet structure with many homogeneous rod-shaped lumps. The preliminary in vitro assays indicated that the EPS could significantly inhibit the adhesion of Escherichia coli O157:H7 to HT-29 cells in competition, replacement, and inhibition assays at a dose of 1...
July 12, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28708031/persistence-of-escherichia-coli-o157-h7-and-total-escherichia-coli-in-feces-and-feedlot-surface-manure-from-cattle-fed-diets-with-and-without-corn-or-sorghum-wet-distillers-grains-with-solubles
#9
Elaine D Berry, James E Wells, Vincent H Varel, Kristin E Hales, Norasak Kalchayanand
Feeding corn wet distillers grains with solubles (WDGS) to cattle can increase the load of Escherichia coli O157:H7 in feces and on hides, but the mechanisms are not fully understood. The objective of these experiments was to examine a role for the persistence of E. coli O157:H7 in the feces and feedlot pen surfaces of cattle fed WDGS. In the first study, feces from steers fed 0, 20, 40, or 60% corn WDGS were inoculated with E. coli O157:H7. The E. coli O157:H7 numbers in feces from cattle fed 0% corn WDGS rapidly decreased (P < 0...
July 14, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28693954/nano-encapsulation-of-chicken-immunoglobulin-igy-in-sodium-alginate-nanoparticles-in%C3%A2-vitro-characterization
#10
Mostafa Bakhshi, Firouz Ebrahimi, Shahram Nazarian, Jamil Zargan, Faeze Behzadi, Dawood Sadeghi Gariz
Controlled delivery of therapeutic agents by alginate nanoparticles became an attractive issue in the gastric organ. Some therapeutic agents such as proteins could not tolerate in severe condition in the gastrointestinal tract. In the present study, four concentrations of a specific IgY as a prophylactic agent against E. coli O157: H7 was entrapped in 0.2% w/v sodium alginate nanoparticles by ionic gelation method. Depending on the IgY concentration entrapment efficacy was 28.31-99.84%. The physicochemical and structural characteristics of free and IgY-loaded Alg NPs revealed that the individual particles exhibited a spherical shape with a diameter of 45-85 nm, and a negatively charged surface with a zeta potential value of 26-36 mV...
July 7, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28674066/contribution-of-the-chromosomal-ccdab-operon-to-bacterial-drug-tolerance
#11
Kritika Gupta, Arti Tripathi, Alishan Sahu, Raghavan Varadarajan
One of the first identified and best studied toxin-antitoxin systems in Escherichia coli is the F-plasmid based CcdAB system. This system is involved in plasmid maintenance through post-segregational killing. More recently, ccdAB homologs have been found on the chromosome, including in pathogenic strains of E. coli and other bacteria. However the functional role of chromosomal ccdAB genes, if any, has remained unclear. We show that both the native ccd operon of the E.coliO157 strain, as well as the ccd operon from the F- plasmid, when inserted on the E...
July 3, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28672173/prevalence-of-verocytotoxigenic-escherichia-coli-strains-isolated-from-raw-beef-in-southern-italy
#12
Gaia Nobili, Ilenia Franconieri, Gianfranco La Bella, Maria Grazia Basanisi, Giovanna La Salandra
Verocytotoxin (VT)-producing Escherichia coli (VTEC) are a significant foodborne public health hazard, where most human infections are associated with six serogroups (O157, O26, O103, O145, O111 and O104). VTEC was the fourth most commonly reported zoonosis in the EU in 2015, with 5901 confirmed human cases. Ruminant animals, including cattle, are a major reservoir of VTEC. The consumption of VTEC-contaminated animal-derived foodstuffs, especially undercooked ground beef, is an important transmission route...
June 28, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28663823/sequencing-a-piece-of-history-complete-genome-sequence-of-the-original-escherichia-coli-strain
#13
Karl A Dunne, Roy R Chaudhuri, Amanda E Rossiter, Irene Beriotto, Douglas F Browning, Derrick Squire, Adam F Cunningham, Jeffrey A Cole, Nicholas Loman, Ian R Henderson
In 1885, Theodor Escherich first described the Bacillus coli commune, which was subsequently renamed Escherichia coli. We report the complete genome sequence of this original strain (NCTC 86). The 5 144 392 bp circular chromosome encodes the genes for 4805 proteins, which include antigens, virulence factors, antimicrobial-resistance factors and secretion systems, of a commensal organism from the pre-antibiotic era. It is located in the E. coli A subgroup and is closely related to E. coli K-12 MG1655. E...
March 2017: Microbial Genomics
https://www.readbyqxmd.com/read/28656639/comparison-of-immunomagnetic-separation-beads-for-detection-of-six-non-o157-shiga-toxin-producing-escherichia-coli-serogroups-in-different-matrices
#14
Autumn L Kraft, David W Lacher, Weilin L Shelver, Julie S Sherwood, Teresa M Bergholz
Immunomagnetic separation used with culture based methods has been a useful technique in the detection of pathogens. However, previous studies have not answered many of the necessary questions for real world applications. The objective of this study was to assess the efficacy of different IMS bead types in recovery of the correct serogroup from a mixture of big six non-O157 STEC strains. To determine the impact of different matrices on recovery, samples of sterile phosphate buffered saline (PBS), sterile and non-sterile cattle feces, ground beef, and lettuce were inoculated with 10 CFU ml(-1) mixture of isolates representing the six serogroups...
June 28, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28655830/correction-for-luzader-et-al-eutr-is-a-direct-regulator-of-genes-that-contribute-to-metabolism-and-virulence-in-enterohemorrhagic-escherichia-coli-o157-h7
#15
Deborah H Luzader, David E Clark, Laura A Gonyar, Melissa M Kendall
No abstract text is available yet for this article.
July 15, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28653930/identification-and-growth-optimization-of-a-marine-bacillus-dk1-sa11-having-potential-of-producing-broad-spectrum-antimicrobial-compounds
#16
Muhammad Naseem Khan, Hong Lin, Meng Li, Jingxue Wang, Zulfiqar Ali Mirani, Seema Ismat Khan, Muhammad Aslam Buzdar, Imran Ali, Khalid Jamil
Control of harmful bacteria in food, aquaculture, pharmaceuticals, agriculture, hospitals and recreation water pools are of great global concern. Marine bacteria are an enormous source of bio-controlling agents. The aim of this study was to identify and optimize the growth conditions including effect of different biotic and abiotic factors on antimicrobial activity of strain DK1-SA11 isolated from Qingdao Bay of China Yellow Sea. Microscopic characterization, API® 20E and 50 CHB kit base carbohydrates utilization, 16S rDNA and DNA gyrB gene sequencing studies identified the bacterium as Bacillus subtilis subsp...
May 2017: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28653733/effects-of-heat-treatment-on-the-antioxidative-and-anti-inflammatory-properties-of-orange-by-products
#17
Chien-Chun Li, Hui-Jin Hsu, Yu-Shiang Wang, Jennifer Cassidy, Shiowshuh Sheen, Shih-Chuan Liu
This study investigated the changes in the functional components, antioxidative activities, antibacterial activities, anti-inflammatory activities of orange (Citrus sinensis (L.) Osbeck) by-products (OBP) on heat treatment at 50 and 100 °C (hereafter denoted 50D and 100D extracts, respectively). Optimal heating conditions were also investigated. The total phenolic content, flavonoid content and antioxidative activities of OBP extracts significantly increased on heat treatment. The lag time of Cu(2+)-induced oxidation of human LDL was increased by 2...
July 19, 2017: Food & Function
https://www.readbyqxmd.com/read/28649305/emerging-microbiota-during-cold-storage-and-temperature-abuse-of-ready-to-eat-salad%C3%A2
#18
Karin Söderqvist, Omneya Ahmed Osman, Cecilia Wolff, Stefan Bertilsson, Ivar Vågsholm, Sofia Boqvist
Introduction: Ready-to-eat (RTE) leafy vegetables have a natural leaf microbiota that changes during different processing and handling steps from farm to fork. The objectives of this study were (i) to compare the microbiota of RTE baby spinach and mixed-ingredient salad before and after seven days of storage at 8°C or 15°C; (ii) to explore associations between bacterial communities and the foodborne pathogens Listeria monocytogenes, pathogenic Yersinia enterocolitica, and pathogen model organism Escherichia coli O157:H7 gfp+ when experimentally inoculated into the salads before storage; and (iii) to investigate if bacterial pathogens may be detected in the 16S rRNA amplicon dataset...
2017: Infection Ecology & Epidemiology
https://www.readbyqxmd.com/read/28643674/microbiological-and-serological-control-of-escherichia-coli-o157-h7-in-kindergarten-staff-in-buenos-aires-city-and-suburban-areas
#19
Romina J Fernández-Brando, María Marta Amaral, Andrés E Ciocchini, Leticia V Bentancor, Jorge A Trelles, Marcelo Da Rocha, Martín Landriel, Mariana Ugarte, Gabriel Briones, Cristina Ibarra, Marina S Palermo
Shiga toxin (Stx)-producing Escherichia coli (STEC) infections are implicated in the development of the life-threatening hemolytic-uremic syndrome (HUS). Despite the magnitude of the social and economic problems caused by HUS, no licensed vaccine or effective therapy is currently available for human use. Prevention of STEC infections continues being the most important measure to reduce HUS incidence. This is especially true for Argentina where HUS incidence among children is extremely high and shows an endemic pattern...
2017: Medicina
https://www.readbyqxmd.com/read/28636835/use-of-bacteriophages-to-control-escherichia-coli-o157-h7-in-domestic-ruminants-meat-products-and-fruits-and-vegetables
#20
Lili Wang, Kunli Qu, Xiaoyu Li, Zhenhui Cao, Xitao Wang, Zhen Li, Yaxiong Song, Yongping Xu
Escherichia coli O157:H7 is an important foodborne pathogen that causes severe bloody diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome. Ruminant manure is a primary source of E. coli O157:H7 contaminating the environment and food sources. Therefore, effective interventions targeted at reducing the prevalence of fecal excretion of E. coli O157:H7 by cattle and sheep and the elimination of E. coli O157:H7 contamination of meat products as well as fruits and vegetables are required. Bacteriophages offer the prospect of sustainable alternative approaches against bacterial pathogens with the flexibility of being applied therapeutically or for biological control purposes...
June 21, 2017: Foodborne Pathogens and Disease
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