Read by QxMD icon Read


Peisheng Liang, Wangyong Zhu, Tianjun Lan, Qian Tao
Saliva secretion disorder is one of the most common symptoms in primary Sjögren syndrome (pSS). Salivary biomarkers related to saliva secretion disorder were identified in a pSS murine model, NOD/ShiLtJ mouse, using differential proteomic analysis. Candidate biomarkers were screened with Kyoto Encyclopedia of Genes and Genomes pathway analysis and validated in saliva and salivary glands by western blotting and immunohistochemistry (IHC). Biological functions were detected using ingenuity pathway analysis (IPA)...
March 12, 2018: Journal of Pharmaceutical and Biomedical Analysis
Bhaskar Mitra, Rene Lametsch, Tolga Akcan, Jorge Ruiz-Carrascal
The impact of thermal processing on meat proteins oxidation was investigated. Cooking treatments included 58 °C for either 72 min or 17 h (mimicking low temperature-long time sous vide cooking), 80, 98 and 160 °C for 72 min (mimicking common cooked meat products, stewing and roasting, respectively) and 118 °C for 8 min (autoclaving). Tryptophan degradation, fluorescent oxidation products, free thiol content and α-aminoadipic and γ-glutamic semialdehydes were tracked. For all of them, there was a consistent trend to increased levels of oxidative damage with higher cooking temperatures and longer cooking times, although the extent varied from one indicator to another...
March 10, 2018: Meat Science
Mafalda Santos, Patricia M Pereira, A Sofia Varanda, Joana Carvalho, Mafalda Azevedo, Denisa D Mateus, Nuno Mendes, Patricia Oliveira, Fábio Trindade, Marta Teixeira Pinto, Renata Bordeira-Carriço, Fátima Carneiro, Rui Vitorino, Carla Oliveira, Manuel A S Santos
Deregulation of tRNAs, aminoacyl-tRNA synthetases and tRNA modifying enzymes are common in cancer, raising the hypothesis that protein synthesis efficiency and accuracy (mistranslation) are compromised in tumors. We show here that human colon tumors and xenograft tumors produced in mice by two epithelial cancer cell lines mistranslate 2- to 4-fold more frequently than normal tissue. To clarify if protein mistranslation plays a role in tumor biology, we expressed mutant Ser-tRNAs that misincorporate Ser-at-Ala (frequent error) and Ser-at-Leu (infrequent error) in NIH3T3 cells and investigated how they responded to the proteome instability generated by the amino acid misincorporations...
March 20, 2018: RNA Biology
Salvador Martínez-Bartolomé, J Alberto Medina-Aunón, Miguel Ángel López-García, Carmen González-Tejedo, Gorka Prieto, Rosana Navajas, Emilio Salazar-Donate, Carolina Fernandez-Costa, John R Yates, Juan Pablo Albar
Mass spectrometry-based proteomics has evolved into a high-throughput technology where numerous large-scale datasets are generated from diverse analytical platforms. Furthermore, several scientific journals and funding agencies have emphasized the storage of proteomics data in public repositories to facilitate its evaluation, inspection and reanalysis. As a consequence, public proteomics data repositories are growing rapidly. However, tools are needed to integrate multiple proteomics datasets in order to compare different experimental features or to perform quality control analysis...
March 20, 2018: Journal of Proteome Research
Zewdu Hora, Solomon Zewdu Altaye, Abebe Jenberie Wubie, Jianke Li
The honeybee is one of the most valuable insect pollinators, which plays a key role in pollinating wild vegetation and agricultural crops, with significant contribution to world food production. Although honeybees have long been studied as model for social evolution, the molecular level of honeybee biology remained poorly understood until the year 2006. With the available honeybee genome sequence, technological advancements in protein separation, mass spectrometry, and bioinformatics, wide ranges of honeybee biology such as developmental biology, physiology, behavior, neurobiology, and immunology are explored to a new depth at molecular and biochemical levels...
March 20, 2018: Journal of Agricultural and Food Chemistry
Nuray Erin, Nur Ogan, Azmi Yerlikaya
PURPOSE: Metastatic breast cancer is resistant to many conventional treatments and novel therapeutic targets are needed. We previously isolated subsets of 4T1 murine breast cancer cells which metastasized to liver (4TLM), brain (4TBM), and heart (4THM). Among these cells, 4TLM is the most aggressive one, demonstrating mesenchymal phenotype. Here we compared secreted proteins from 4TLM, 4TBM, and 4THM cells and compared with that of hardly metastatic 67NR cells to detect differentially secreted factors involved in organ-specific metastasis...
March 20, 2018: Breast Cancer Research and Treatment
Yin Yuan, Xiu-Yue Xu, Jie Lao, Xin Zhao
Nerve transfer is the most common treatment for total brachial plexus avulsion injury. After nerve transfer, the movement of the injured limb may be activated by certain movements of the healthy limb at the early stage of recovery, i.e., trans-hemispheric reorganization. Previous studies have focused on functional magnetic resonance imaging and changes in brain-derived neurotrophic factor and growth associated protein 43, but there have been no proteomics studies. In this study, we designed a rat model of total brachial plexus avulsion injury involving contralateral C7 nerve transfer...
February 2018: Neural Regeneration Research
Keunsub Lee, Kan Wang
During the last decade, small noncoding RNAs (ncRNAs) have emerged as essential post-transcriptional regulators in bacteria. Nearly all important physiological and stress responses are modulated by ncRNA regulators, such as riboswitches, trans-acting small RNAs (sRNAs), and cis-antisense RNAs. Recently, three RNA-seq studies identified a total of 1534 candidate ncRNAs from Agrobacterium tumefaciens, a pathogen and biotechnology tool for plants. Only a few ncRNAs have been functionally characterized in A. tumefaciens, and some of them appear to be involved in virulence...
March 20, 2018: Current Topics in Microbiology and Immunology
Shengjian Yuan, Ling Chen, Quan Liu, Yan Zhou, Jingfang Yang, Deng Deng, Hongping Li, Yingfei Ma
In this study, two bacteriophage isolates, AhSzq-1 and AhSzw-1 that specifically infect Aeromonas hydrophila strain KT998822, were isolated from seawater and characterized. One-step growth curves showed that the latent period of AhSzq-1 and AhSzw-1 are 50 min and 60 min, respectively. The sequence similarities between AhSzq-1 and AhSzw-1 were 88% at the DNA and 83% at the protein level, suggesting that these two phages are representatives of two different species. The virion morphology, DNA genome size and terminal repeats of these two phages are similar to those of viruses classified as T5virus phages...
March 19, 2018: Archives of Virology
Michael Lehrer, Anindya Bhadra, Sathvik Aithala, Visweswaran Ravikumar, Youyun Zheng, Basak Dogan, Emerlinda Bonaccio, Elizabeth S Burnside, Elizabeth Morris, Elizabeth Sutton, Gary J Whitman, Jose Net, Kathy Brandt, Marie Ganott, Margarita Zuley, Arvind Rao
Background: Imaging features derived from MRI scans can be used for not only breast cancer detection and measuring disease extent, but can also determine gene expression and patient outcomes. The relationships between imaging features, gene/protein expression, and response to therapy hold potential to guide personalized medicine. We aim to characterize the relationship between radiologist-annotated tumor phenotypic features (based on MRI) and the underlying biological processes (based on proteomic profiling) in the tumor...
January 2018: Oncoscience
Kai Huang, Chuan Fang, Kaikai Yi, Xing Liu, Hongzhao Qi, Yanli Tan, Junhu Zhou, Ying Li, Mingyang Liu, Yuqing Zhang, Jingxuan Yang, Jianning Zhang, Min Li, Chunsheng Kang
Exosomes play critical roles in intercellular communication in both nearby and distant cells in individuals and organs. Polymerase I and transcript release factor (PTRF), also known as Cavin1, has previously been described as a critical factor in caveola formation, and aberrant PTRF expression has been reported in various malignancies. However, the function of PTRF in tumor progression remains controversial, and its role in glioma is poorly understood. In this study, we report that PTRF is associated with malignancy grade and poor prognosis in glioma patients...
2018: Theranostics
Frank Hochholdinger, Caroline Marcon, Jutta A Baldauf, Peng Yu, Felix P Frey
Maize forms a complex root system with structurally and functionally diverse root types that are formed at different developmental stages to extract water and mineral nutrients from soil. In recent years proteomics has been intensively applied to identify proteins involved in shaping the three-dimensional architecture and regulating the function of the maize root system. With the help of developmental mutants, proteomic changes during the initiation and emergence of shoot-borne, lateral and seminal roots have been examined...
2018: Frontiers in Plant Science
Jorge Ruiz-Orera, Pol Verdaguer-Grau, José Luis Villanueva-Cañas, Xavier Messeguer, M Mar Albà
Accumulating evidence indicates that some protein-coding genes have originated de novo from previously non-coding genomic sequences. However, the processes underlying de novo gene birth are still enigmatic. In particular, the appearance of a new functional protein seems highly improbable unless there is already a pool of neutrally evolving peptides that are translated at significant levels and that can at some point acquire new functions. Here, we use deep ribosome-profiling sequencing data, together with proteomics and single nucleotide polymorphism information, to search for these peptides...
March 19, 2018: Nature ecology & evolution
Davoud Mozhdehi, Kelli M Luginbuhl, Joseph R Simon, Michael Dzuricky, Rüdiger Berger, H Samet Varol, Fred C Huang, Kristen L Buehne, Nicholas R Mayne, Isaac Weitzhandler, Mischa Bonn, Sapun H Parekh, Ashutosh Chilkoti
Post-translational modification of proteins is a strategy widely used in biological systems. It expands the diversity of the proteome and allows for tailoring of both the function and localization of proteins within cells as well as the material properties of structural proteins and matrices. Despite their ubiquity in biology, with a few exceptions, the potential of post-translational modifications in biomaterials synthesis has remained largely untapped. As a proof of concept to demonstrate the feasibility of creating a genetically encoded biohybrid material through post-translational modification, we report here the generation of a family of three stimulus-responsive hybrid materials-fatty-acid-modified elastin-like polypeptides-using a one-pot recombinant expression and post-translational lipidation methodology...
March 19, 2018: Nature Chemistry
Cheng-Ta Yang, Jhy-Ming Li, Li-Fu Li, Yu-Shien Ko, Jeng-Ting Chen
The overexpression of stomatin-like protein-2 (SLP-2) is commonly observed in non-small cell lung cancer (NSCLC) cells. In the present study, we transfected a number of NSCLC cells with an SLP-2 shRNA-expressing vector (AdSLP2i) and examined its possible effects on cell growth and apoptosis. We found that suppression of SLP-2 expression inhibited cell growth, and that the apoptosis induced by SLP-2 suppression was correlated with decreased survivin protein expression. Moreover, the reduced survivin expression was found to be associated with reduced β-catenin nuclear localization and appeared not to be modulated through the AKT signaling pathway...
March 19, 2018: Cell Death & Disease
Ding Xu, Lichen Yang, Yu Wang, Gejiao Wang, Christopher Rensing, Shixue Zheng
Elemental selenium nanoparticles (SeNPs) are useful in medicine, environmental remediation and in material science. Biosynthesized SeNPs (BioSeNPs) by bacteria are cheap, eco-friendly and have a lower cytotoxicity in comparison with chemically synthesized ones. Organic matters were found to cap on the surface of BioSeNPs, but the functions were still not entirely clear. The purified BioSeNPs were coated in a thick layer of organic substrates observed by transmission electron microscopy (TEM). Fourier Transform Infrared (FT-IR) and quantitative detection of the coating agents showed that one gram of purified BioSeNPs bound 1069 mg proteins, 23 mg carbohydrates and only very limited amounts of lipids...
March 19, 2018: Scientific Reports
Montserrat Baldan-Martin, Juan A Lopez, Nerea Corbacho-Alonso, Paula J Martinez, Elena Rodriguez-Sanchez, Laura Mourino-Alvarez, Tamara Sastre-Oliva, Tatiana Martin-Rojas, Raul Rincón, Eva Calvo, Jesus Vazquez, Fernando Vivanco, Luis R Padial, Gloria Alvarez-Llamas, Gema Ruiz-Hurtado, Luis M Ruilope, Maria G Barderas
The evaluation of cardiovascular (CV) risk is based on equations derived from epidemiological data in individuals beyond the limits of middle age such as the Framingham and SCORE risk assessments. Lifetime Risk calculator (QRisk® ), estimates CV risk throughout a subjects' lifetime, allowing those. A more aggressive and earlier intervention to be identified and offered protection from the consequences of CV and renal disease. The search for molecular profiles in young people that allow a correct stratification of CV risk would be of great interest to adopt preventive therapeutic measures in individuals at high CV risk...
March 19, 2018: Scientific Reports
Kai Li, Yue Zhang, Yuwei Zhang, Wenqing Jiang, Junhui Shen, Song Xu, Daozhang Cai, Jie Shen, Bin Huang, Mangmang Li, Qiancheng Song, Yu Jiang, Anling Liu, Xiaochun Bai
OBJECTIVES: To investigate the role of tyrosine kinase Fyn in the development of osteoarthritis (OA) and the underlying mechanisms, and to define whether targeting Fyn could prevent OA in mice. METHODS: Cartilage samples from normal and aged mice were analysed with proteome-wide screening. Fyn expression was examined with immunofluorescence in human and age-dependent or experimental mouse OA cartilage samples. Experimental OA in Fyn-knockout mice was induced by destabilisation of the medial meniscus...
March 19, 2018: Annals of the Rheumatic Diseases
Kiyomichi Imamura, Hikari Yoshitane, Kazuki Hattori, Mitsuo Yamaguchi, Kento Yoshida, Takenori Okubo, Isao Naguro, Hidenori Ichijo, Yoshitaka Fukada
Daily rhythms of behaviors and physiologies are generated by the circadian clock, which is composed of clock genes and the encoded proteins forming transcriptional/translational feedback loops (TTFLs). The circadian clock is a self-sustained oscillator and flexibly responds to various time cues to synchronize with environmental 24-h cycles. However, the key molecule that transmits cellular stress to the circadian clockwork is unknown. Here we identified apoptosis signal-regulating kinase (ASK), a member of the MAPKKK family, as an essential mediator determining the circadian period and phase of cultured cells in response to osmotic changes of the medium...
March 19, 2018: Proceedings of the National Academy of Sciences of the United States of America
Thomas W M Crozier, Michele Tinti, Richard J Wheeler, Tony Ly, Michael A J Ferguson, Angus I Lamond
We describe a single-step centrifugal elutriation method to produce synchronous G1-phase procyclic trypanosomes at a scale amenable for proteomic analysis of the cell cycle. Using ten-plex tandem mass tag (TMT) labelling and mass spectrometry (MS)-based proteomics technology, the expression levels of 5,325 proteins were quantified across the cell cycle in this parasite. Of these, 384 proteins were classified as cell-cycle regulated and subdivided into nine clusters with distinct temporal regulation. These groups included many known cell cycle regulators in trypanosomes, which validates the approach...
March 19, 2018: Molecular & Cellular Proteomics: MCP
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"