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https://www.readbyqxmd.com/read/29054075/evaluation-of-immunological-markers-of-ovine-vaginal-irritation-implications-for-preclinical-assessment-of-non-vaccine-hiv-preventive-agents
#1
Gregg N Milligan, Gracie Vargas, Kathleen L Vincent, Yong Zhu, Nigel Bourne, Massoud Motamedi
The presence of genital inflammatory responses and a compromised vaginal epithelial barrier have been linked to an increased risk of HIV acquisition. It is important to assure that application of candidate microbicides designed to limit HIV transmission will not cause these adverse events. We previously developed high resolution in vivo imaging methodologies in sheep to assess epithelial integrity following vaginal application of a model microbicide, however characterization of genital inflammation in sheep has not been previously possible...
October 13, 2017: Journal of Reproductive Immunology
https://www.readbyqxmd.com/read/29052357/critical-sized-full-thickness-skin-defect-regeneration-using-ovine-small-intestinal-submucosa-with-or-without-mesenchymal-stem-cells-in-rat-model
#2
Morteza Rashtbar, Jamshid Hadjati, Jafar Ai, Sadegh Shirian, Issa Jahanzad, Mahmoud Azami, Shiva Asadpuor, Esmaeil Sadroddiny
Decellularized extracellular matrices (ECM) based materials are routinely used for a variety of clinical applications. Hereof, in vivo application of decellularized ovine small intestinal submucosal (DOSIS) layer as, a scaffold is yet to be investigated. In this study, the effectiveness of the DOSIS scaffold, with or without rat bone marrow mesenchymal stem cells (BM-MSCs), in full-thickness wound healing of critical-sized defect was experimentally studied in a rat model. The experimental groups included; group I (control), group II (DOSIS), and group III (BM-MSCs-seeded DOSIS)...
October 20, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/29051303/transport-associated-pathway-responses-in-ovine-fetal-membranes-to-changes-in-amniotic-fluid-dynamics
#3
Cecilia Y Cheung, Debra F Anderson, Robert A Brace
Current evidence suggests that amniotic fluid volume (AFV) is actively regulated by vesicular transport of amniotic fluid outward across the amnion and into the underlying fetal vasculature in the placenta. Our objective was to determine whether gene expression profiles of potential stimulators, inhibitors, and mediators of vesicular transport are altered in response to changes in intramembranous absorption (IMA) rate. Samples of ovine amnion and chorion were obtained from fetal sheep with normal, experimentally reduced or increased AFVs and IMA rates...
November 2017: Physiological Reports
https://www.readbyqxmd.com/read/29051057/mannheimia-haemolytica-a2-secretes-different-proteases-into-the-culture-medium-and-in-outer-membrane-vesicles
#4
Gerardo Ramírez Rico, Moisés Martínez-Castillo, Cynthia González-Ruíz, Sarahí Luna-Castro, Mireya de la Garza
Respiratory diseases in ruminants have a significantly negative impact on the worldwide economy. The bacterium Mannheimia haemolytica is involved in pneumonic infections in bovine and ovine. In gram-negative bacteria, six secretion systems related to the colonization process and host tissue damage have been reported. In addition, in the last two decades, the production of outer membrane vesicles has been studied as a different bacterial strategy to release virulence factors, such as exotoxins, lipopolysaccharides, and proteases...
October 17, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29046495/molecular-epidemiological-survey-and-genetic-characterization-of-ovine-gammaherpesvirus-2-in-mongolian-livestock
#5
Nyamsuren Ochirkhuu, Satoru Konnai, Raadan Odbileg, Shiro Murata, Kazuhiko Ohashi
Sheep-associated malignant catarrhal fever (SA-MCF), caused by ovine gammaherpesvirus-2 (OvHV-2), is a fatal disease in all ruminants. The epidemiological survey and molecular characterization of OvHV-2 in Mongolian livestock were performed. Of 928 blood samples, 14 were positive for OvHV-2 in sheep and native cattle from Tsenkher County and in sheep from Lun County. Phylogenetic analyses revealed that the tegument gene of OvHV-2 sequences from Mongolian animals is identical to that in animals from Egypt, India, and Turkey, and is 98...
October 19, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29044433/pregnancy-induced-changes-in-metabolome-and-proteome-in-ovine-uterine-flushings
#6
Jared J Romero, Bethany E Liebig, Corey D Broeckling, Jessica E Prenni, Thomas R Hansen
Mass spectrometry (MS) approaches were used herein to identify metabolites and proteins in uterine flushings (UF) that may contribute to nourishing the conceptus. Ovine uteri collected on Day 12 of the estrous cycle (n = 5 ewes exposed to vasectomized ram) or Days 12 (n = 4), 14 (n = 5), or 16 (n = 5) of pregnancy (bred with fertile ram) were flushed using buffered saline. Metabolites were extracted using 80% methanol and profiled using ultraperformance liquid chromatography (LC) tandem mass spectrometry. The proteome was examined by digestion with trypsin, followed by the analysis of peptides with LC-MS/MS...
August 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29043108/mysterious-inhibitory-cell-regulator-investigated-and-found-likely-to-be-secretogranin-ii-related
#7
John E Hart, Iain J Clarke, Gail P Risbridger, Ben Ferneyhough, Mónica Vega-Hernández
In the context of a hunt for a postulated hormone that is tissue-mass inhibiting and reproductively associated, there is described probable relatedness to a granin protein. A 7-8 kDa polypeptide candidate (gels/MS) appeared in a bioassay-guided fractionation campaign involving sheep plasma. An N-terminal sequence of 14 amino acids was obtained for the polypeptide by Edman degradation. Bioinformatics and molecular biology failed to illuminate any ovine or non-ovine protein which might relate to this sequence...
2017: PeerJ
https://www.readbyqxmd.com/read/29038101/influence-of-intramyocardial-adipose-tissue-on-the-accuracy-of-endocardial-contact-mapping-of-the-chronic-myocardial-infarction-substrate
#8
Rahul Samanta, Saurabh Kumar, William Chik, Pierre Qian, Michael A Barry, Sara Al Raisi, Abhishek Bhaskaran, Melad Farraha, Fazlur Nadri, Eddy Kizana, Aravinda Thiagalingam, Pramesh Kovoor, Jim Pouliopoulos
BACKGROUND: Recent studies have demonstrated that intramyocardial adipose tissue (IMAT) may contribute to ventricular electrophysiological remodeling in patients with chronic myocardial infarction. Using an ovine model of myocardial infarction, we aimed to determine the influence of IMAT on scar tissue identification during endocardial contact mapping and optimal voltage-based mapping criteria for defining IMAT dense regions. METHOD AND RESULTS: In 7 sheep, left ventricular endocardial and transmural mapping was performed 84 weeks (15-111 weeks) post-myocardial infarction...
October 2017: Circulation. Arrhythmia and Electrophysiology
https://www.readbyqxmd.com/read/29037276/analysis-of-single-nucleotide-polymorphisms-variation-associated-with-important-economic-and-computed-tomography-measured-traits-in-texel-sheep
#9
D Garza Hernandez, S Mucha, G Banos, K Kaseja, K Moore, N Lambe, J Yates, L Bunger
Sheep are an important part of the global agricultural economy. Growth and meat production traits are significant economic traits in sheep. The Texel breed is the most popular terminal sire breed in the UK, mainly selected for muscle growth and lean carcasses. This is a study based on a genome-wide association approach that investigates the links between some economically important traits, including computed tomography (CT) measurements, and molecular polymorphisms in UK Texel sheep. Our main aim was to identify single nucleotide polymorphisms (SNP) associated with growth, carcass, health and welfare traits of the Texel sheep breed...
October 17, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/29034091/phenotypic-and-genotypic-characterization-of-antibiotic-resistance-of-methicillin-resistant-staphylococcus-aureus-isolated-from-hospital-food
#10
Farhad Safarpoor Dehkordi, Hasan Gandomi, Afshin Akhondzadeh Basti, Ali Misaghi, Ebrahim Rahimi
BACKGROUND: Pathogenic biotypes of the Methicillin-resistant Staphylococcus aureus (MRSA) strains are considered to be one of the major cause of food-borne diseases in hospitals. The present investigation was done to study the pattern of antibiotic resistance and prevalence of antibiotic resistance genes of different biotypes of the MRSA strains isolated from various types of hospital food samples. METHODS: Four-hundred and eighty-five raw and cooked hospital food samples were cultured and MRSA strains were identified using the oxacillin and cefoxitin disk diffusion tests and mecA-based PCR amplification...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/29029874/sperm-subpopulations-in-ejaculated-sperm-and-spermatozoa-recovered-from-ovine-epididymides-up-to-48h-after-death
#11
Tácia Gomes Bergstein-Galan, Romildo Romualdo Weiss, Luiz Ernandes Kozicki, Sony Dimas Bicudo
The objectives of this study were threefold: to identify subpopulations of sperm based on the kinetics of frozen/thawed sheep epididymal spermatozoa or semen collected with an artificial vagina; to evaluate the effects on sperm subpopulations in the thawed samples of post mortem storage at room temperature and the addition of 20% of seminal plasma to the freezing extender and to correlate the percentage of subpopulations with gestation rate following artificial intrauterine insemination. The categorization of the subpopulations was based on sperm kinetic data from Computer Assisted Sperm Analysis (CASA)...
October 5, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29024947/human-trophoblast-derived-hydrogen-sulfide-stimulates-placental-artery-endothelial-cell-angiogenesis
#12
Dongbao Chen, Lin Feng, Jennifer K Hodges, Thomas J Lechuga, Honghai Zhang
Endogenous hydrogen sulfide (H2S), mainly synthesized by cystathionine β-synthase (CBS) and cystathionineγ-lyase (CTH), has been implicated in regulating placental angiogenesis; however, the underlying mechanisms are unknown. This study was to test a hypothesis that trophoblasts synthesize H2S to promote placental angiogenesis. Human choriocarcinoma-derived BeWo cells expressed both CBS and CTH proteins while the first trimester villous trophoblast-originated HTR-8/SVneo cells expressed CTH protein only. The H2S producing ability of BeWo cells was significantly inhibited by either inhibitors of CBS (CHH) or CTH (BCA) and that in HTR-8/SVneo cells was inhibited by CHH only...
August 28, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29024514/therapeutic-potential-of-haecs-for-early-achilles-tendon-defect-repair-through-regeneration
#13
Barbara Barboni, Valentina Russo, Valentina Gatta, Nicola Bernabò, Paolo Berardinelli, Annunziata Mauro, Alessandra Martelli, Luca Valbonetti, Aurelio Muttini, Oriana Di Giacinto, Maura Turriani, Antonietta Silini, Giuseppe Calabrese, Michele Abate, Ornella Parolini, Liborio Stuppia, Mauro Mattioli
Cell-based therapy hold great promise for tendon disorders, a widespread debilitating musculoskeletal condition. Even if the cell line remains to be defined, preliminary evidences have proven that amniotic-derived cells possess in vitro and in vivo a great tenogenic potential. The present study investigated the efficacy of transplanted human amniotic epithelial cells (hAECs) by testing their early regenerative properties and mechanisms involved on a validated ovine Achilles tendon partial defect performed on 29 animals...
October 11, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29023489/spatiotemporal-variations-in-gene-expression-histology-and-biomechanics-in-an-ovine-model-of-tendinopathy
#14
Sara Biasutti, Andrew Dart, Margaret Smith, Carina Blaker, Elizabeth Clarke, Leo Jeffcott, Christopher Little
Flexor tendinopathy is a common problem affecting humans and animals. Tendon healing is poorly understood and the outcomes of conservative and surgical management are often suboptimal. While often considered a localized injury, recent evidence indicates that in the short term, tendinopathic changes are distributed widely throughout the tendon, remote from the lesion itself. Whether these changes persist throughout healing is unknown. The aim of this study was to document gene expression, histopathological and biomechanical changes that occur throughout the superficial digital flexor tendon (SDFT) up to 16 weeks post-injury, using an ovine surgical model of tendinopathy...
2017: PloS One
https://www.readbyqxmd.com/read/28988559/ram-semen-deterioration-by-short-term-exposure-to-high-altitude-is-prevented-by-improvement-of-antioxidant-status
#15
E Cofré, O A Peralta, A Raggi, M De Los Reyes, F Sales, A González-Bulnes, V H Parraguez
Ovine reproduction efficiency in herds at high altitude (ha) is lower than that at low altitude (la). In ewes, ha effects are due to hypoxia and oxidative stress. Our aim was to establish the effect of antioxidant vitamin supplementation on semen traits and antioxidant status of rams exposed to short or long time ha. A total of 32 rams native to la (~500 m) were used, 16 were kept at la and the other 16 were brought to ha (~3600 m), where they were placed in the same flock as the ha native rams (n=16). Half of the animals in each group were supplemented daily with vitamins C 600 mg and E 450 IU per os, during the entire experimental period, starting the 4th day after animal's arrival at ha (day 0)...
October 9, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28986745/nutritional-and-metabolic-stressors-on-ovine-oocyte-development-and-granulosa-cell-functions-in-vitro
#16
S Nandi, S K Tripathi, P S P Gupta, S Mondal
The present study was undertaken to study the effect of ammonia, urea, non-esterified fatty acid (NEFA), and β-hydroxybutyric acid (β-OHB) on oocyte development and granulosa cell (GC) growth parameter of ovine (Ovis aries). Ovine oocytes were matured in vitro in the presence of different concentration of ammonia, urea, NEFA, and β-OHB for 24 h, in vitro inseminated and evaluated for cleavage and blastocyst yield. Same concentrations of ammonia, urea, NEFA, and β-OHB were examined on growth parameters and hormone secretion activity of granulosa cells in vitro...
October 6, 2017: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/28983925/a-genome-wide-significant-association-on-chromosome-2-for-footrot-resistance-susceptibility-in-swiss-white-alpine-sheep
#17
A Niggeler, J Tetens, A Stäuble, A Steiner, C Drögemüller
Footrot is one of the most important causes of lameness in global sheep populations and is characterized by a bacterial infection of the interdigital skin. As a multifactorial disease, its clinical representation depends not only on pathogen factors and environmental components but also on the individual resistance/susceptibility of the host. A genetic component has been shown in previous studies; however, so far no causative genetic variant influencing the risk of developing footrot has been identified. In this study, we genotyped 373 Swiss White Alpine sheep, using the ovine high-density 600k SNP chip, in order to run a DNA-based comparison of individuals with known clinical footrot status...
October 5, 2017: Animal Genetics
https://www.readbyqxmd.com/read/28983742/multi-resolution-geometric-modeling-of-the-mitral-heart-valve-leaflets
#18
Amir H Khalighi, Andrew Drach, Robert C Gorman, Joseph H Gorman, Michael S Sacks
An essential element of cardiac function, the mitral valve (MV) ensures proper directional blood flow between the left heart chambers. Over the past two decades, computational simulations have made marked advancements toward providing powerful predictive tools to better understand valvular function and improve treatments for MV disease. However, challenges remain in the development of robust means for the quantification and representation of MV leaflet geometry. In this study, we present a novel modeling pipeline to quantitatively characterize and represent MV leaflet surface geometry...
October 5, 2017: Biomechanics and Modeling in Mechanobiology
https://www.readbyqxmd.com/read/28982935/the-evolution-of-interferon-tau
#19
REVIEW
Alan D Ealy, Lydia K Wooldridge
Thirty years ago, a novel type I interferon (IFN) was identified by molecular cloning of cDNA libraries constructed from RNA extracted from ovine and bovine pre-implantation embryos. This protein was eventually designated as IFN-tau (IFNT) to highlight its trophoblast-dependent expression. IFNT function is not immune related. Instead, it interacts with the maternal system to initiate the establishment and maintenance of pregnancy. This activity is indispensable for the continuation of pregnancy. Our review will describe how IFNT evolved from other type I IFNs to function in this new capacity...
November 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28982297/articular-cartilage-repair-with-mesenchymal-stem-cells-after-chondrogenic-priming-a-pilot-study
#20
Troy Bornes, Adetola Adesida, Nadr Jomha
Bone marrow-derived mesenchymal stromal stem cells (BMSCs) are a promising cell source for treating articular cartilage defects. The objective of this study was to assess a protocol that involved autologous transplantation of BMSCs into full-thickness cartilage defects in sheep following isolation, expansion and a short period (4 days) of chondrogenic priming. The impact of oxygen tension during pre-implantation culture was investigated. It was hypothesized that chondrogenically primed BMSCs would produce superior cartilaginous repair tissue relative to control defects, and that culture under hypoxia would yield improved repair tissue in comparison to normoxia...
October 6, 2017: Tissue Engineering. Part A
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