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https://www.readbyqxmd.com/read/28343363/sample-pooling-for-real-time-pcr-detection-and-virulence-determination-of-the-footrot-pathogen-dichelobacter-nodosus
#1
Sara Frosth, Ulrika König, Ann-Kristin Nyman, Anna Aspán
Dichelobacter nodosus is the principal cause of ovine footrot and strain virulence is an important factor in disease severity. Therefore, detection and virulence determination of D. nodosus is important for proper diagnosis of the disease. Today this is possible by real-time PCR analysis. Analysis of large numbers of samples is costly and laborious; therefore, pooling of individual samples is common in surveillance programs. However, pooling can reduce the sensitivity of the method. The aim of this study was to develop a pooling method for real-time PCR analysis that would allow sensitive detection and simultaneous virulence determination of D...
March 25, 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/28339937/uterine-artery-leptin-receptors-during-the-ovarian-cycle-and-pregnancy-regulate-angiogenesis-in-ovine-uterine-artery-endothelial-cells
#2
Vladimir E Vargas, Rosalina Villalon Landeros, Gladys E Lopez, Jing Zheng, Ronald R Magness
Leptin regulates body weight, reproductive functions, blood pressure, endothelial function, and fetoplacental angiogenesis. Compared to the luteal phase, the follicular phase and pregnancy are physiological states of elevated estrogen, angiogenesis, and uterine blood flow (UBF). Little is known concerning leptin and its receptors (OB-R) for regulating uterine artery (UA) angiogenesis. We : 1) Ex vivo expression of OB-R in UA endothelium (UAendo) and UA vascular smooth muscle (UAvsm) is elevated in pregnant vs...
March 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28338081/a-distinct-bacterial-dysbiosis-associated-skin-inflammation-in-ovine-footrot
#3
Grazieli Maboni, Adam Blanchard, Sara Frosth, Ceri Stewart, Richard Emes, Sabine Tötemeyer
Ovine footrot is a highly prevalent bacterial disease caused by Dichelobacter nodosus and characterised by the separation of the hoof horn from the underlying skin. The role of innate immune molecules and other bacterial communities in the development of footrot lesions remains unclear. This study shows a significant association between the high expression of IL1β and high D. nodosus load in footrot samples. Investigation of the microbial population identified distinct bacterial populations in the different disease stages and also depending on the level of inflammation...
March 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28332004/social-setting-social-rank-and-hpa-axis-response-in-cynomolgus-monkeys
#4
Vanessa A Jimenez, Daicia C Allen, Megan N McClintick, Kathleen A Grant
RATIONALE: Hypothalamic-pituitary-adrenal (HPA) axis activity under different social settings in non-human primates is understudied. OBJECTIVE: The aim of this study is to evaluate the response of pituitary-adrenal hormones (adrenocorticotropic hormone (ACTH) and cortisol) to pharmacological challenges of the HPA axis in male cynomolgus macaques under different social settings. METHODS: Male cynomolgus macaques (Macaca fascicularis, n = 11) were individually (A) and socially housed (B) in alternation, over consecutive months, in an ABA design...
March 22, 2017: Psychopharmacology
https://www.readbyqxmd.com/read/28331489/reversible-atrial-fibrillation-following-crotalinae-envenomation
#5
Dan Quan, Kenneth Zurcher
BACKGROUND: Cardiotoxicity is a documented complication of Crotalinae envenomation. Reported cardiac complications following snake envenomation have included acute myocardial infarction, electrocardiogram abnormalities and arrhythmias. Few reports exist describing arrhythmia induced by viper envenomation and to our knowledge none describe arrhythmia induced by Crotalinae envenomation. This report concerns the first known case of atrial fibrillation precipitated by rattlesnake bite. CASE PRESENTATION: A 73-year-old Caucasian man with a past medical history of hypertension, hyperlipidemia, type 1 diabetes mellitus, and a baseline first-degree atrioventricular block presented to the emergency department following a rattlesnake bite to his left lower leg...
2017: Journal of Venomous Animals and Toxins Including Tropical Diseases
https://www.readbyqxmd.com/read/28329164/large-animal-model-of-functional-tricuspid-regurgitation-in-pacing-induced-end-stage-heart-failure
#6
Marcin Malinowski, Alistair G Proudfoot, David Langholz, Lenora Eberhart, Michael Brown, Hans Schubert, Jeremy Wodarek, Tomasz A Timek
OBJECTIVES: Functional tricuspid regurgitation (FTR) is common in patients with advanced heart failure and frequently complicates left ventricular assist device implantation yet remains poorly understood. We set out to establish large animal model of FTR that could serve as a research platform to investigate the pathogenesis of FTR associated with end-stage heart failure. METHODS: : Through right thoracotomy, ten adult sheep underwent implantation of pacemaker with epicardial LV lead, five sonomicrometry crystals on the right ventricle, and left and right ventricular telemetry pressure sensors during a beating heart off-pump procedure...
February 21, 2017: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/28325734/does-maternal-fetal-transfer-of-creatine-occur-in-pregnant-sheep
#7
Syed Baharom, Robert De Matteo, Stacey Ellery, Paul Della Gatta, Clinton R Bruce, Greg M Kowalski, Nadia Hale, Hayley Dickinson, Richard Harding, David Walker, Rodney J Snow
Our aim was to determine the disposition of creatine in ovine pregnancy, and whether creatine is transferred across the placenta from mother to fetus. Pregnant ewes received either: (i) a continuous intravenous infusion of creatine monohydrate or saline from 122 to 131 days gestation, with maternal and fetal arterial blood and amniotic fluid samples collected daily for creatine analysis and fetal tissues collected at necropsy at 133 days for analysis of creatine content, or; (ii) a single systemic bolus injection of (13)C labelled creatine monohydrate at 130 days of gestation, with maternal and fetal arterial blood, uterine vein blood and amniotic fluid samples collected prior to, and for 4 hours post injection and analysed for creatine, creatine isotopic enrichment, and guanidinoacetic acid (GAA, precursor of creatine) concentrations...
March 21, 2017: American Journal of Physiology. Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28323562/corynebacterium-pseudotuberculosis-infection-in-patagonian-huemul-hippocamelus-bisulcus
#8
Nelly Morales, Dennis Aldridge, Andrea Bahamonde, Julio Cerda, Claudio Araya, Rodrigo Muñoz, María Esther Saldías, Claudio Lecocq, Marcela Fresno, Pedro Abalos, Patricio Retamal
Corynebacterium pseudotuberculosis is an intracellular bacteria and the etiologic agent of caseous lymphadenitis in domestic and wildlife species. We report C. pseudotuberculosis infection in Patagonian huemul ( Hippocamelus bisulcus ) from the Cerro Castillo National Reserve, Region of Aysen, Chile. Subcutaneous abscesses in the abdominal and pectoral regions from two animals were sampled and bacteriologic isolation was performed. In both cases, we isolated a C. pseudotuberculosis strain belonging to the ovine genotype...
March 21, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28321790/peste-des-petits-ruminants-infection-in-domestic-ruminants-in-sudan
#9
K S Intisar, Y H Ali, M A Haj, M A T Sahar, M M Shaza, A M Baraa, O M Ishag, Y M Nouri, K M Taha, E M Nada, A M Ahmed, A I Khalafalla, G Libeau, A Diallo
The existence of peste des petits ruminants (PPR) in domestic ruminants and camels in Sudan during 2008-2012 was investigated. Lung tissues and serum samples were randomly collected from sheep, goats, cattle, and camels at different areas of Sudan. A total of 12,384 serum samples were collected from clinically healthy 7413 sheep, 1988 camels, 1501 cattle, 1459 goats, and 23 gazelles at different areas in the Sudan. They were examined for PPR antibodies using competitive ELISA (cELISA). The overall detected seroprevalence of PPR in tested sera was 49...
March 20, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28320890/a-biophysical-vascular-bubble-model-for-devising-decompression-procedures
#10
Ran Arieli, Abraham Marmur
Vascular bubble models, which present a realistic biophysical approach, hold great promise for devising suitable diver decompression procedures. Nanobubbles were found to nucleate on a flat hydrophobic surface, expanding to form bubbles after decompression. Such active hydrophobic spots (AHS) were formed from lung surfactants on the luminal aspect of ovine blood vessels. Many of the phenomena observed in these bubbling vessels correlated with those known to occur in diving. On the basis of our previous studies, which proposed a new model for the formation of arterial bubbles, we now suggest the biophysical model presented herein...
March 2017: Physiological Reports
https://www.readbyqxmd.com/read/28320598/molecular-characterization-of-ureaplasmas-isolated-from-reproductive-tract-of-goats-and-sheep-from-brazil
#11
Rosângela C Oliveira, Maurício Yamaguti, Lucas M Marques, Melissa Buzinhani, Huber Rizzo, Enoch Brandão de Souza Meira-Junior, Ana Márcia S Guimarães, Lilian Gregory, Jorge Timenetsky
Ovine/caprine ureaplasmas have not yet been assigned a species designation, but they have been classified into nine serotypes. Herein ureaplasmas were searched for in 120 samples of vulvo vaginal mucous from sheep and 98 samples from goats at 17 farms. In addition, semen samples were collected from 11 sheep and 23 goats. The recovered ureaplasma were from sheep and goats from animals without any reproductive disorder symptoms, but not all animals presented positive cultures. In sheep, 17 (68%) cultures of vulvovaginal mucous were positive for ureaplasma and 11 (27%) samples of semen presented positive cultures in animals with clinical signs of orchitis, balanoposthitis or low sperm motility...
February 20, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28318592/caprine-and-ovine-greek-dairy-products-the-official-german-method-generates-false-positive-results-due-to-%C3%AE%C2%BA-casein-gene-polymorphism
#12
V Tsartsianidou, D Triantafillidou, N Karaiskou, P Tarantili, G Triantafillidis, E Georgakis, A Triantafyllidis
Caseins are widely used for species identification of dairy products. Isoelectric focusing (IEF) of para-κ-casein peptide is used as the official German method for the differentiation between caprine (isoform A) and ovine (isoform B) dairy products, based on their different isoelectric points. The discrimination between Greek goat and ewe dairy products using IEF has, however, been shown to be problematic because of the existence of the ewe isoform in milk from Greek indigenous dairy goats. This could be due to nucleotide polymorphisms within the goat κ-casein gene of Greek indigenous breeds, which alter the isoelectric point of the para-κ-casein peptide and lead to false positive results...
March 16, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28318586/prediction-and-repeatability-of-milk-coagulation-properties-and-curd-firming-modeling-parameters-of-ovine-milk-using-fourier-transform-infrared-spectroscopy-and-bayesian-models
#13
A Ferragina, C Cipolat-Gotet, A Cecchinato, M Pazzola, M L Dettori, G M Vacca, G Bittante
The aim of this study was to apply Bayesian models to the Fourier-transform infrared spectroscopy spectra of individual sheep milk samples to derive calibration equations to predict traditional and modeled milk coagulation properties (MCP), and to assess the repeatability of MCP measures and their predictions. Data consisted of 1,002 individual milk samples collected from Sarda ewes reared in 22 farms in the region of Sardinia (Italy) for which MCP and modeled curd-firming parameters were available. Two milk samples were taken from 87 ewes and analyzed with the aim of estimating repeatability, whereas a single sample was taken from the other 915 ewes...
March 16, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28318100/abrogation-of-red-blood-cell-g6pd-enzyme-activity-through-heat-treatment-development-of-survey-material-for-the-uk-neqas-g6pd-scheme
#14
D R Roper, B De la Salle, V Soni, K Fletcher, J A Green
INTRODUCTION: Participation in external quality assessment (EQA) is central to the maintenance of high-quality laboratory results in patient diagnosis and clinical trials. Laboratories in the TAF112582 DETECTIVE study (ClinicalTrials.gov identifier: NCT01376167) are enrolled in the United Kingdom National Quality Assessment Scheme (UK NEQAS) for glucose-6-phosphate dehydrogenase (G6PD) quantitative assay, which utilizes ovine (sheep) blood as a readily available source of apparently G6PD-deficient survey material...
March 20, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28302567/alpha2-adrenoceptor-agonists-trigger-prolactin-signaling-in-breast-cancer-cells
#15
Lilian Fedra Castillo, Ezequiel M Rivero, Vincent Goffin, Isabel Alicia Lüthy
Breast cancer is the most frequent malignancy among women worldwide. We have described the expression of α2-adrenoceptors in breast cancer cell lines, associated with increased cell proliferation and tumor growth. A mitogenic autocrine/paracrine loop of prolactin (Prl) has been described in breast cancer cells. We hypothesized that the α2-adrenergic enhancement of proliferation could be mediated, at least in part, by this Prl loop. In both T47D and MCF-7 cell lines, the incubation with the α2-adrenergic agonist dexmedetomidine significantly increased Prl release into the culture medium (measured by the Nb2 bioassay), this effect being reversed by the α2-adrenergic antagonist rauwolscine...
March 14, 2017: Cellular Signalling
https://www.readbyqxmd.com/read/28301581/assessing-tn5-and-sleeping-beauty-for-transpositional-transgenesis-by-cytoplasmic-injection-into-bovine-and-ovine-zygotes
#16
R J Bevacqua, R Fernandez-Martin, N G Canel, A Gibbons, D Texeira, F Lange, G Vans Landschoot, V Savy, O Briski, M I Hiriart, E Grueso, Z Ivics, O Taboga, W A Kues, S Ferraris, D F Salamone
Transgenic domestic animals represent an alternative to bioreactors for large-scale production of biopharmaceuticals and could also provide more accurate biomedical models than rodents. However, their generation remains inefficient. Recently, DNA transposons allowed improved transgenesis efficiencies in mice and pigs. In this work, Tn5 and Sleeping Beauty (SB) transposon systems were evaluated for transgenesis by simple cytoplasmic injection in livestock zygotes. In the case of Tn5, the transposome complex of transposon nucleic acid and Tn5 protein was injected...
2017: PloS One
https://www.readbyqxmd.com/read/28293985/immortalized-sheep-microglial-cells-are-permissive-to-a-diverse-range-of-ruminant-viruses
#17
James B Stanton, Beryl Swanson, Edith Orozco, Juan F Muñoz-Gutiérrez, James F Evermann, Julia F Ridpath
BACKGROUND: Ruminants, including sheep and goats (small ruminants), are key agricultural animals in many parts of the world. Infectious diseases, including many viral diseases, are significant problems to efficient production of ruminants. Unfortunately, reagents tailored to viruses of ruminants, and especially small ruminants, are lacking compared to other animals more typically used for biomedical research. OBJECTIVE: The purpose of this study was to determine the permissibility of a stably immortalized, sheep microglial cell line to viruses that are reported to infect ruminants: bovine viral diarrhea virus (BVDV), bovine herpesvirus 1 (BoHV-1), small ruminant lentiviruses (SRLV), and bovine respiratory syncytial virus (BRSV)...
December 2017: Veterinary Quarterly
https://www.readbyqxmd.com/read/28293789/development-of-an-ovine-model-for-training-in-vaginal-surgery-for-pelvic-organ-prolapse
#18
A Mansoor, S Curinier, S Campagne-Loiseau, L Platteeuw, B Jacquetin, B Rabischong
INTRODUCTION AND HYPOTHESIS: This aim of this study was to evaluate the feasibility and usefulness of an ovine model for training in vaginal surgery. METHODS: Four senior urogynaecological surgeons and five residents attended five sessions of vaginal surgery for pelvic organ prolapse (POP) in five old multiparous female sheep. Urogynaecological examinations were performed and measurements similar to the POP-Q classification in humans were obtained. Standard POP surgical procedures with and without mesh were performed...
March 14, 2017: International Urogynecology Journal
https://www.readbyqxmd.com/read/28289843/micro-ct-evaluation-of-asymmetrical-ovine-intervertebral-disc-height-loss-from-surgical-approach
#19
Christopher Ludtka, Stefan Schwan, Andrea Friedmann, Walther Brehm, Ingo Wiesner, Felix Goehre
PURPOSE: The primary goal of this study is to clearly define and evaluate new intervertebral disc height parameters in analysing the morphological pathology of disc degeneration for application in damage model and regeneration therapy development, as well as applying traditional variables to 3-D characterization methods. METHODS: A posterolateral surgical approach was used to induce disc degeneration in an ovine model. At 12-months post-operation, sheep vertebral segments were removed and characterized using micro-CT to evaluate disc height parameters in regard to injury localization...
March 13, 2017: European Spine Journal
https://www.readbyqxmd.com/read/28286624/a-biomechanical-comparison-of-4-strand-and-5-strand-anterior-cruciate-ligament-graft-constructs
#20
Matthew L Broadhead, Animesh A Singla, Nicky Bertollo, David Broe, William R Walsh
Hamstring tendon autografts are used for reconstruction of the anterior cruciate ligament. This study tested the hypothesis that a 5-strand hamstring autograft construct is superior in strength to a 4-strand construct. Four-strand and 5-strand tendon grafts constructs were prepared from ovine flexor tendons and then tested in a uniaxial electromechanical load system with suspensory fixation. The 4-strand and 5-strand constructs were pre-conditioned, stress-relaxed and loaded to ultimate failure. Stress-relaxation, stiffness and ultimate load were compared using a one-way ANOVA...
February 20, 2017: Orthopedic Reviews
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