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https://www.readbyqxmd.com/read/29675269/translation-of-remote-control-regenerative-technologies-for-bone-repair
#1
Hareklea Markides, Jane S McLaren, Neil D Telling, Noura Alom, E'atelaf A Al-Mutheffer, Richard O C Oreffo, Andrew Zannettino, Brigitte E Scammell, Lisa J White, Alicia J El Haj
The role of biomechanical stimuli, or mechanotransduction, in normal bone homeostasis and repair is understood to facilitate effective osteogenesis of mesenchymal stem cells (MSCs) in vitro. Mechanotransduction has been integrated into a multitude of in vitro bone tissue engineering strategies and provides an effective means of controlling cell behaviour towards therapeutic outcomes. However, the delivery of mechanical stimuli to exogenous MSC populations, post implantation, poses a significant translational hurdle...
2018: NPJ Regenerative Medicine
https://www.readbyqxmd.com/read/29673420/neurologic-complications-associated-with-transdermal-placement-of-intrathecal-catheters-in-sheep
#2
Gabrielle C Musk, Nolan J McDonnell, Mark Newman, Matthew W Kemp
A study using an ovine model of transdermal intrathecal catheterization was planned to investigate the neurotoxicity ofmagnesium sulfate. Nonpregnant Merino cross ewes (n = 8; age, 5 y; weight, 55.0 ± 6.5 kg) were anesthetized for placementof a lumbar intrathecal catheter. The study protocol defined a 5-d recovery period after introduction of the catheter before theadministration of test substances (2 mL of 0.9% saline or 50 or 150 mg MgSO4) followed by euthanasia 1 wk later. Although 3sheep successfully completed the study as planned, one of the remaining 5 sheep was withdrawn when the catheter was accidentally dislodged 2 d after anesthesia; another was withdrawn because of persistent neurologic deficits of the left hindlimb and intense pruritus during the first 24 h after placement of the catheter; and the remaining 3 animals experienced unacceptable complications within the first 4 h of administration of the test substance...
April 19, 2018: Comparative Medicine
https://www.readbyqxmd.com/read/29673373/chorioamnionitis-neuroinflammation-and-injury-timing-is-key-in-the-preterm-ovine-fetus
#3
Ruth Gussenhoven, Rob J J Westerlaken, Daan R M G Ophelders, Alan H Jobe, Matthew W Kemp, Suhas G Kallapur, Luc J Zimmermann, Per T Sangild, Stanislava Pankratova, Pierre Gressens, Boris W Kramer, Bobbi Fleiss, Tim G A M Wolfs
BACKGROUND: Antenatal infection (i.e., chorioamnionitis) is an important risk factor for adverse neurodevelopmental outcomes after preterm birth. Destructive and developmental disturbances of the white matter are hallmarks of preterm brain injury. Understanding the temporal effects of antenatal infection in relation to the onset of neurological injury is crucial for the development of neurotherapeutics for preterm infants. However, these dynamics remain unstudied. METHODS: Time-mated ewes were intra-amniotically injected with lipopolysaccharide at 5, 12, or 24 h or 2, 4, 8, or 15 days before preterm delivery at 125 days gestational age (term ~ 150 days)...
April 19, 2018: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/29673353/modulation-of-ovine-sbd-1-expression-by-saccharomyces-cerevisiae-in-ovine-ruminal-epithelial-cells
#4
Xin Jin, Man Zhang, Xue-Min Zhu, Yan-Ru Fan, Chen-Guang Du, Hua-Er Bao, Siri-Guleng Xu, Qiao-Zhen Tian, Yun-He Wang, Yin-Feng Yang
BACKGROUND: The ovine rumen is involved in host defense responses and acts as the immune interface with the environment. The ruminal mucosal epithelium plays an important role in innate immunity and secretes antimicrobial innate immune molecules that have bactericidal activity against a variety of pathogens. Defensins are cationic peptides that are produced by the mucosal epithelia and have broad-spectrum antimicrobial activity. Sheep β-defensin-1 (SBD-1) is one of the most important antibacterial peptides in the rumen...
April 19, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/29669335/antenatal-corticosteroid-exposure-disrupts-myelination-in-the-auditory-nerve-of-preterm-sheep
#5
Judith Rittenschober-Böhm, Jennifer Rodger, Alan H Jobe, Suhas G Kallapur, Dorota A Doherty, Boris W Kramer, Matthew S Payne, Michael Archer, Christian Rittenschober, John P Newnham, Yuichiro Miura, Angelika Berger, Stephen G Matthews, Matthew W Kemp
BACKGROUND: Antenatal corticosteroids (ACS) improve preterm neonatal outcomes. However, uncertainty remains regarding the safety of ACS exposure for the developing fetus, particularly its neurosensory development. OBJECTIVES: We investigated the effect of single and multiple ACS exposures on auditory nerve development in an ovine model of pregnancy. METHODS: Ewes with a single fetus (gestational age [GA] 100 days) received an intramuscular injection of 150 mg medroxyprogesterone-acetate, followed by intramuscular (i) betamethasone (0...
April 17, 2018: Neonatology
https://www.readbyqxmd.com/read/29668949/molecular-evidence-for-a-novel-species-of-babesia-in-unfed-rhipicephalus-sanguineus-sensu-lato-acari-ixodidae
#6
Sezayi Ozubek, Munir Aktas
This study reports a novel species of Babesia in unfed Rhipicephalus sanguineus sensu lato and its phylogenetic relationship to other species. One-hundred and forty DNA samples extracted from tick pools comprising 5,403 unfed R. sanguineus s.l. ticks were screened for Babesia. Overall Babesia infection was detected in female, male, and nymph pools with an infection rate maximum likelihood estimate (MLE) of 1.98 (CI 0.65-4.74), 0.50 (CI 0.03-2.40), and 0.07 (CI 0.01-0.21), respectively. Three adult female tick pools (MLE 1...
April 16, 2018: Journal of Medical Entomology
https://www.readbyqxmd.com/read/29668912/ovine-placental-steroid-synthesis-and-metabolism-in-late-gestation
#7
Lawrence P Reynolds, Erin L Legacki, C Jo Corbin, Joel S Caton, Kimberly A Vonnahme, Scott Stanley, Alan J Conley
Steroid synthesis is required for pregnancy maintenance and for parturition but comparatively little is known about the major metabolic routes that influence circulating concentrations. Dietary intake changes progesterone and estradiol concentrations in pregnant ewes but whether this reflects placental synthesis is unknown. Progesterone metabolism by 5alpha-reduction is a major metabolic route in other species and can influence the onset of parturition. Therefore, studies were conducted to 1) determine placental enzyme activity, progesterone and estradiol measured by immuno-assay in late gestation ewes on low, moderate and high nutritional planes, 2) to assess the significance of 5alpha-reduction of progesterone in determining progesterone concentrations in late gestation ewes (gestation day 145) given finasteride to inhibit 5alpha-reductase metabolism...
April 14, 2018: Biology of Reproduction
https://www.readbyqxmd.com/read/29668843/effects-of-catecholamines-on-secretion-of-interferon-tau-and-expression-of-genes-for-synthesis-of-polyamines-and-apoptosis-by-ovine-trophectoderm
#8
Mohammed A Elmetwally, Yasser Lenis, Wanjin Tang, Guoyao Wu, Fuller W Bazer
Ovine trophectoderm (oTr1) cells were used to investigate effects of epinephrine (EP), norepinephrine (NE) and dopamine (DA) on their proliferation, migration and adhesion, secretion of interferon tau (IFNT), and expression of genes for synthesis of polyamines and apoptosis. Expression of mRNAs for agmatinase (AGMAT), arginine decarboxylase (ADC), ornithine decarboxylase (ODC1) and solute carrier family 7 (SLC7A1) cationic amino acid transporter, Y + system), member 1 increased (P < 0.05) in oTr1 cells in response to EP and DA...
April 12, 2018: Biology of Reproduction
https://www.readbyqxmd.com/read/29668309/posttraumatic-osteoarthritis-development-and-progression-in-an-ovine-model-of-partial-anterior-cruciate-ligament-transection-and-effect-of-repeated-intra-articular-methylprednisolone-acetate-injections-on-early-disease
#9
Kristen I Barton, Bryan J Heard, Johnathan L Sevick, C Ryan Martin, S M Mehdi Shekarforoush, May Chung, Yamini Achari, Cyril B Frank, Nigel G Shrive, David A Hart
BACKGROUND: Partial anterior cruciate ligament (p-ACL) ruptures are a common injury of athletes. However, few preclinical models have investigated the natural history and treatment of p-ACL injuries. PURPOSE: To (1) demonstrate whether a controlled p-ACL injury model (anteromedial band transection) develops progressive gross morphological and histological posttraumatic osteoarthritis (PTOA)-like changes at 20 and 40 weeks after the injury and (2) investigate the efficacy of repeated (0, 5, 10, and 15 weeks) intra-articular injections of methylprednisolone acetate (MPA; 80 mg/mL) in the mitigation of potential PTOA-like changes after p-ACL transection...
April 1, 2018: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/29666222/first-report-of-prion-related-protein-gene-prnt-polymorphisms-in-cattle
#10
Yong-Chan Kim, Byung-Hoon Jeong
Prion diseases are caused by structural changes in normal prion protein (PrPC ). The prion gene family includes four members: prion protein ( PRNP ), prion-like protein ( PRND ), shadow of PRNP ( SPRN ) and prion-related protein ( PRNT ). Genetic association studies of prion diseases and the other genes in the prion gene family, except for PRNT , have been performed in cattle. Our previous studies indicated that the distribution of PRNP promoter polymorphisms related with bovine spongiform encephalopathy susceptibility is significantly different in Hanwoo (Korean native cattle) and Holstein cattle...
April 17, 2018: Veterinary Record
https://www.readbyqxmd.com/read/29663394/polymorphism-in-ovine-anxa9-gene-and-physic-chemical-properties-and-the-fraction-of-protein-in-milk
#11
Ewa Pecka-Kiełb, Ewa Czerniawska-Piątkowska, Inga Kowalewska-Łuczak, Milan Vasil
BACKGROUND: Annexin A9 (ANXA9) is a specific fatty acid transport protein. ANXA9 gene is expressed in various tissues, including secretory tissue and mammary glands. The association between three SNPs of the ANXA9 gene and sheep's milk compositions was assessed. RESULTS: Genotype analysis was performed with the use of PCR-RFLP method. The studied ANXA9 polymorphisms had the following MAF (Major Allele Frequency): SNP1: allele G 0,66; SNP2: allele G 0,54; SNP3: allele C 0,57...
April 16, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29661099/mir-125b-contributes-to-ovarian-granulosa-cell-apoptosis-through-targeting-bmpr1b-a-major-gene-for-sheep-prolificacy
#12
Yilong Yao, Anwaier Reheman, Yefei Xu, Qifa Li
Bone morphogenetic protein receptor 1B (BMPR1B) is a major gene for ovine ovulation rate and litter size, which plays a crucial role in follicle development. However, its role and regulation in the ovine granulosa cells (GCs) are unclear. Here, we showed that silencing of BMPR1B enhanced cell apoptosis, whereas overexpression of BMPR1B inhibited cell apoptosis in the ovine GCs. Luciferase reporter assays revealed that BMPR1B is a target of miR-125b, and miR-125b reduced BMPR1B messenger RNA and protein levels in the ovine GCs by directly binding to the 3' untranslated region of the ovine BMPR1B gene...
January 1, 2018: Reproductive Sciences
https://www.readbyqxmd.com/read/29660821/like-sheep-like-humans-right-ventricular-remodelling-in-a-preterm-born-ovine-model
#13
Afifah Mohamed, Paul Leeson, Adam J Lewandowski
Preterm birth affects more than 10% of births worldwide, with over 80% of these individuals born moderately preterm; between 32-36 weeks' gestation. This article is protected by copyright. All rights reserved.
April 16, 2018: Journal of Physiology
https://www.readbyqxmd.com/read/29660626/tubulin-posttranslational-modifications-in-in-vitro-matured-prepubertal-and-adult-ovine-oocytes
#14
E Serra, S Succu, F Berlinguer, C Porcu, G G Leoni, S Naitana, S D Gadau
Microtubules (MTs), polymers of alpha/beta-tubulin heterodimers, are involved in crucial functions in eukaryotic cells. MTs physiology can be influenced by a variety of post-translational modifications (PTMs), including tyrosination, detyrosination, delta 2 modification, acetylation, polyglutamylation, polyglycylation. In mammalian oocytes, MTs are essential for meiosis, regulating the formation of meiotic spindle and chromosomes movements. Considering that the patterns of tubulin PTMs (tyrosination, detyrosination, acetylation, polyglutamylation and delta 2 modification) have not been investigated in ovine oocytes, this study has been designed to investigate their presence and quantification in in vitro matured (IVM) adult and prepubertal ovine oocytes...
April 7, 2018: Theriogenology
https://www.readbyqxmd.com/read/29660557/theileria-lestoquardi-in-sudan-is-highly-diverse-and-genetically-distinct-from-that-in-oman
#15
Hoyam Awad, Salama Al-Hamidhi, A M El Hussein, Younis Mohammed Zein Yousif, Khalid M Taha, D A Salih, William Weir, Hamza A Babiker
Malignant ovine theileriosis is a severe tick-borne protozoan disease of sheep and other small ruminants which is widespread in sub-Saharan Africa and the Middle East. The disease is of considerable economic importance in Sudan as the export of livestock provides a major contribution to the gross domestic product of this country. Molecular surveys have demonstrated a high prevalence of sub-clinical infections of Theileria lestoquardi, the causative agent, among small ruminants. No information is currently available on the extent of genetic diversity and genetic exchange among parasites in different areas of the country...
April 13, 2018: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/29659410/in-vivo-degradation-of-crosslinked-hyaluronic-acid-fillers-by-exogenous-hyaluronidases
#16
Garrett T Shumate, Rajesh Chopra, Derek Jones, Darin J Messina, Christopher K Hee
BACKGROUND: An advantage of hyaluronic acid (HA)-based fillers is reversibility. OBJECTIVE: To evaluate the ability of 2 hyaluronidases to degrade 3 HA-based fillers using a novel in vivo model. MATERIALS AND METHODS: Rats were injected with 3 HA fillers (HYC-24L+, VYC-20L, and RES-L) to create a projecting bolus. After 4 days, recombinant human hyaluronidase (HX) or ovine hyaluronidase (VIT) was administered at (1) varying doses (5 U, 10 U, or 30 U per 0...
April 13, 2018: Dermatologic Surgery: Official Publication for American Society for Dermatologic Surgery [et Al.]
https://www.readbyqxmd.com/read/29658494/multi-resolution-simulation-of-focused-ultrasound-propagation-through-ovine-skull-from-a-single-element-transducer
#17
Kyungho Yoon, Wonhye Lee, Phillip Croce, Amanda Cammalleri, Seung-Schik Yoo
Transcranial focused ultrasound (tFUS) is emerging as a non-invasive brain stimulation modality. Complicated interactions between acoustic pressure waves and osseous tissue introduce many challenges in the accurate targeting of an acoustic focus through the cranium. Image-guidance accompanied by a numerical simulation is desired to predict the intracranial acoustic propagation through the skull; however, such simulations typically demand sophisticated computation, which warrants an expedited processing method to provide on-site feedback for the user in guiding the acoustic focus to a particular brain region...
April 16, 2018: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/29657415/semi-nested-polymerase-chain-reaction-based-detection-of-babesia-spp-in-small-ruminants-from-northwest-of-iran
#18
Ahad Bazmani, Amir Abolhooshyar, Abbas Imani-Baran, Hamid Akbari
Aim: The present study aimed to detect Babesia ovis and Babesia motasi in the blood samples of sheep and goats from Northwest of Iran by the semi-nested polymerase chain reaction (PCR) technique. Materials and Methods: A total of 166 whole blood samples (including 123 sheep and 43 goats) were collected. In the first stage, the PCR was performed to amplify a piece of 18S rRNA gene of Babesia and Theileria genera. Then, semi-nested PCR was carried out on all PCR products to differentiate B...
March 2018: Veterinary World
https://www.readbyqxmd.com/read/29656994/an-ex-vivo-abomasal-ovine-model-to-study-the-immediate-immune-response-in-the-context-of-haemonchus-contortus-larval-stage
#19
Saeed El-Ashram, Cuiping Li, Fathi Abouhajer, Rashid Mehmood, Ibrahim Al Nasr, Yinghui Zhang, Tang Lu, Ding Yili, Xun Suo, Zhang Haoji, Zhili Li, Shujian Huang
We have set up an ex vivo ovine abomasal model, which can mimic the multicellular process to explore the early steps in haemonchine nematode infection using RNA-seq technology. Ovine abomasal explants were collected for histological and transcriptional analysis and supernatants collected to quantitate lactate dehydrogenase (LDH) enzymes. Atotal of 233 were substantially induced genes between L4 -inoculated and uninoculated-control tissues, respectively. However, a total of 14 were considerably down-regulated genes between the 51 aforementioned tissues...
April 30, 2018: Veterinary Parasitology
https://www.readbyqxmd.com/read/29628922/the-applied-development-of-a-tiered-multilocus-sequence-typing-mlst-scheme-for-dichelobacter-nodosus
#20
Adam M Blanchard, Keith A Jolley, Martin C J Maiden, Tracey J Coffey, Grazieli Maboni, Ceri E Staley, Nicola J Bollard, Andrew Warry, Richard D Emes, Peers L Davies, Sabine Tötemeyer
Dichelobacter nodosus ( D. nodosus ) is the causative pathogen of ovine footrot, a disease that has a significant welfare and financial impact on the global sheep industry. Previous studies into the phylogenetics of D. nodosus have focused on Australia and Scandinavia, meaning the current diversity in the United Kingdom (U.K.) population and its relationship globally, is poorly understood. Numerous epidemiological methods are available for bacterial typing; however, few account for whole genome diversity or provide the opportunity for future application of new computational techniques...
2018: Frontiers in Microbiology
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