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https://www.readbyqxmd.com/read/27908771/molecular-detection-and-genetic-characterization-of-babesia-theileria-and-anaplasma-amongst-apparently-healthy-sheep-and-goats-in-the-central-region-of-turkey
#1
Mo Zhou, Shinuo Cao, Ferda Sevinc, Mutlu Sevinc, Onur Ceylan, Sepil Ekici, Charoonluk Jirapattharasate, Paul Franck Adjou Moumouni, Mingming Liu, Guanbo Wang, Aiko Iguchi, Patrick Vudriko, Hiroshi Suzuki, Xuenan Xuan
Babesia spp., Theileria spp. and Anaplasma spp. are significant tick-borne pathogens of livestock globally. In this study, we investigated the presence and distribution of Babesia ovis, Theileria ovis and Anaplasma ovis in 343 small ruminants (249 sheep and 94 goats) from 13 towns in the Central Anatolia region of Turkey using species-specific PCR assays. The PCR were conducted using the primers based on the B. ovis ssu rRNA (BoSSUrRNA), T. ovis ssu rRNA (ToSSUrRNA) and A. ovis major surface protein 4 (AoMSP4) genes, respectively...
November 16, 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27908246/inhibition-of-chaperonin-groel-by-a-monomer-of-ovine-prion-protein-and-its-oligomeric-forms
#2
S S Kudryavtseva, Y Y Stroylova, I A Zanyatkin, T Haertle, V I Muronetz
The possibility of inhibition of chaperonin functional activity by amyloid proteins was studied. It was found that the ovine prion protein PrP as well as its oligomeric and fibrillar forms are capable of binding with the chaperonin GroEL. Besides, GroEL was shown to promote amyloid aggregation of the monomeric and oligomeric PrP as well as PrP fibrils. The monomeric PrP was shown to inhibit the GroEL-assisted reactivation of the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The oligomers of PrP decelerate the GroEL-assisted reactivation of GAPDH, and PrP fibrils did not affect this process...
October 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/27902399/truncation-of-the-enzootic-nasal-tumor-virus-envelope-protein-cytoplasmic-tail-increases-env-mediated-fusion-and-infectivity
#3
Scott R Walsh, Jondavid G de Jong, Jacob P van Vloten, María Carla Rosales Gerpe, Lisa A Santry, Sarah K Wootton
Enzootic nasal tumor virus (ENTV) and Jaagsiekte sheep retrovirus (JSRV) are highly related ovine betaretroviruses that induce nasal and lung tumors in small ruminants, respectively. While the ENTV and JSRV envelope (Env) glycoproteins mediate virus entry using the same cellular receptor, the GPI-linked protein hyaluronoglucosaminidase, ENTV Env pseudovirions mediate entry into cells from a much more restricted range of species than do JSRV Env pseudovirions. Unlike JSRV Env, ENTV Env does not induce cell fusion at pH 5...
November 11, 2016: Journal of General Virology
https://www.readbyqxmd.com/read/27900401/vaginal-wall-weakness-in-parous-ewes-a-potential-preclinical-model-of-pelvic-organ-prolapse
#4
Natharnia Young, Anna Rosamilia, John Arkwright, Joseph Lee, Miranda Davies-Tuck, Joan Melendez, Jerome Werkmeister, Caroline E Gargett
INTRODUCTION AND HYPOTHESIS: Ewes develop pelvic organ prolapse (POP) and may be a suitable model for preclinical studies evaluating cell-based therapies for POP. The aim of this study was to establish a clinical score of vaginal weakness and to compare POP Quantification System (POP-Q) values in conscious nulliparous and parous ewes and determine whether ewes are a suitable POP model. METHODS: Ewes (n = 114) were examined while conscious, without sedation, and standing in a V conveyer by adapting the human POP-Q measurement...
November 29, 2016: International Urogynecology Journal
https://www.readbyqxmd.com/read/27898848/rapid-communication-isolation-of-glucose-transporters-and-in-bovine-uteroplacental-tissues-from-days-16-to-50-of-gestation
#5
M S Crouse, J S Caton, K J McLean, P P Borowicz, L P Reynolds, C R Dahlen, B W Neville, A K Ward
Glucose transporter solute carrier family 2 member 14 () is a duplicon of glucose transporter solute carrier family 2 member 3 () with a 95% shared homology to and has not previously been isolated in ruminant uteroplacental tissues. The transporter has been previously isolated in Holstein heifer uterine epithelium but not in ovine epithelium. We hypothesized that and its duplicon would be found in bovine uteroplacental tissues and that maternal nutrition and day of gestation would impact mRNA expression of and ...
October 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/27895290/comparison-of-phenotypic-and-genotypic-profiles-among-caprine-and-ovine-mycoplasma-ovipneumoniae-strains
#6
Z Maksimović, C De la Fe, J Amores, Á Gómez-Martín, M Rifatbegović
Mycoplasma ovipneumoniae (Movp) is considered to be one of the most important mycoplasmas causing respiratory disease in small ruminants. Most epidemiologic and characterisation studies have been conducted on strains collected from sheep. Information on the presence and characteristics of Movp in healthy and pneumonic goats is limited. Phenotypic or genotypic differences between sheep and goat isolates have never been studied. The objective of our study was to characterise and compare the similarities and differences between caprine and ovine Movp strains isolated from affected and asymptomatic animals in order to elucidate phenotypic and genotypic variability...
November 28, 2016: Veterinary Record
https://www.readbyqxmd.com/read/27890083/anticoccidial-efficacy-of-naringenin-and-a-grapefruit-peel-extract-in-growing-lambs-naturally-infected-with-eimeria-spp
#7
Agustín Pérez-Fonseca, Yazmin Alcala-Canto, Abdelfattah Z M Salem, Aldo B Alberti-Navarro
The current study aimed to determine the anti-Eimeria efficacy of an extract of grapefruit peels (GF) and commercial naringenin (NAR) in naturally-infected lambs, as well as the influence of these flavonoids on the oxidative status during ovine coccidiosis. Pharmacokinetic profiles were also determined. Extracts were administered per os to Eimeria naturally infected growing lambs during 90 consecutive days. The commercial anticoccidial drug toltrazuril (TTZ) was included in this trial as a standard. Twenty-four lambs were divided into four groups: NAR, lambs given a daily dose of 5mg of a commercial naringenin extract of 98% higher purity per kg body weight; GF, lambs that recived a daily dose of 5mg of ethanolic extract of grapefruit peels per kg body weight; TTZ, lambs treated with 20mg of toltrazuril/kg body weight on days 0 and 15 of the experiment; and CTRL, untreated lambs that received daily dose of 30ml of water...
December 15, 2016: Veterinary Parasitology
https://www.readbyqxmd.com/read/27888772/adrenocortical-sensitivity-moderated-by-ongoing-stress-predicts-drinking-intensity-in-alcohol-dependent-men
#8
Bryon Adinoff, David Leonard, Julianne Price, Martin A Javors, Robrina Walker, E Sherwood Brown, Hong Xiao, Uma Rao
Allostatic load from both environmental stressors and persistent glucocorticoid secretion has been associated with disease severity in alcohol dependence. Heightened relapse risk and/or drinking severity, in particular, may be a reaction to alcohol- and withdrawal-induced changes in physiological stress response systems coupled with ongoing life stress, although their shared contributions upon drinking severity have not been assessed. To investigate the combined contribution of hypothalamic-pituitary-adrenal (HPA) reactivity and environmental stressors (e...
November 5, 2016: Psychoneuroendocrinology
https://www.readbyqxmd.com/read/27884436/distribution-of-foxp3-t-cells-in-the-liver-and-hepatic-lymph-nodes-of-goats-and-sheep-experimentally-infected-with-fasciola-hepatica
#9
A Escamilla, R Zafra, J Pérez, T N McNeilly, I L Pacheco, L Buffoni, F J Martínez-Moreno, V Molina-Hernández, A Martínez-Moreno
Foxp3 regulatory T cells (Tregs) are now considered to play a key role in modulation of immune responses during parasitic helminth infections. Immunomodulation is a key factor in Fasciola hepatica infection; however, the distribution and role of Foxp3(+) Tregs cells have not been investigated in F. hepatica infected ruminants. The aim of this study was to evaluate the presence of Foxp3(+) Tregs in the liver and hepatic lymph nodes from experimentally infected sheep and goats during acute and chronic stages of infection...
October 30, 2016: Veterinary Parasitology
https://www.readbyqxmd.com/read/27879512/uhmwpe-sublaminar-wires-in-posterior-spinal-instrumentation-stability-and-biocompatibility-assessment-in-an-ovine-pilot-study
#10
Rob Bogie, Laura Voss, Jacobus J Arts, Arno Lataster, Paul C Willems, Boudewijn Brans, Lodewijk W van Rhijn, Tim J M Welting
STUDY DESIGN: An animal study. OBJECTIVE: To explore ultra-high molecular weight polyethylene (UHMWPE) sublaminar wires in spinal surgery and to assess stability and biocompatibility of the UHMWPE instrumentation in an ovine model. SUMMARY OF BACKGROUND DATA: Sublaminar wiring is a well-established technique in segmental scoliosis surgery. However, during introduction and/or removal of the metal sublaminar wires, neurological problems can occur...
December 2016: Clinical Spine Surgery
https://www.readbyqxmd.com/read/27878974/evidence-for-circulation-of-bovine-viral-diarrhoea-virus-type-2c-in-ruminants-in-southern-italy
#11
N Decaro, M S Lucente, G Lanave, P Gargano, V Larocca, M Losurdo, L Ciambrone, P A Marino, A Parisi, F Casalinuovo, C Buonavoglia, G Elia
Recently, bovine viral diarrhoea virus type 2c (BVDV-2c) was responsible for a severe outbreak in cattle in northern Europe. Here, we present the results of an epidemiological survey for pestiviruses in ruminants in southern Italy. Pooled serum samples were obtained from 997 bovine, 800 ovine, 431 caprine and eight bubaline farms, and pestiviral RNA was detected by molecular methods in 44 farms consisting of 16 cattle and one buffalo herds and of 21 sheep and six goat flocks. Twenty-nine and 15 farms were infected by BVDV-1 and BVDV-2 strains, respectively...
November 23, 2016: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/27878913/response-of-an-ovine-laryngeal-injury-model-to-a-novel-fibrosis-inhibitor
#12
Jacqueline Allen
BACKGROUND: Vocal fold injury results in severe voice alteration that limits occupational function and social interaction. An ovine model of laryngeal injury has been developed, validated and utilized to examine laryngeal wound healing and the effect of a novel collagen inhibitor (halofuginone) on surgical wound healing. The study design includes basic research and animal model. METHODS: An ovine laryngeal model was utilized to study controlled vocal fold injury and healing...
November 23, 2016: ANZ Journal of Surgery
https://www.readbyqxmd.com/read/27877177/comparative-transcriptomic-analyses-of-differentially-expressed-genes-in-transgenic-melatonin-biosynthesis-ovine-hiomt-gene-in-switchgrass
#13
Shan Yuan, Cong Guan, Sijia Liu, Yanhua Huang, Danyang Tian, Xin Cui, Yunwei Zhang, Fuyu Yang
Melatonin serves pleiotropic functions in prompting plant growth and resistance to various stresses. The accurate biosynthetic pathway of melatonin remains elusive in plant species, while the N-acetyltransferase and O-methyltransferase were considered to be the last two key enzymes during its biosynthesis. To investigate the biosynthesis and metabolic pathway of melatonin in plants, the RNA-seq profile of overexpression of the ovine HIOMT was analyzed and compared with the previous transcriptome of transgenic oAANAT gene in switchgrass, a model plant for cellulosic ethanol production...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27876986/prevalence-and-pathological-lesions-of-ovine-cysticercosis-in-slaughtered-sheep-in-western-iran
#14
Mohammad Hashemnia, Yasser Shahbazi, Ghasem Frajani Kish
Cysticercusovis, the intermediate stage of a canine tapeworm, Taenia ovis, produces cystic lesions in the skeletal and cardiac muscle of sheep which, if numerous, will result in the condemnation of an entire carcass. This study was carried out between March 2013 and March 2014 to estimate the prevalence of Taenia oviscysticercosis in sheep slaughtered at the Kermanshah municipal abattoir, in western Iran. Of 69,198 sheep examined, 833 (1.27 %) were infected with cysticerci of Taenia ovis. The prevalence of C...
December 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/27876977/a-study-on-prevalence-and-identification-of-ovine-theileria-and-babesia-infection-in-zabol-using-pcr-method
#15
Neda Sharifi, Maryam Ganjali, Reza Nabavi, Dariush Saadati
Infection with Babesia and Theileria Causes high mortality and economical losses in livestock and has a relatively high prevalence in Iran. In Zabol, animals are susceptible to this disease because of presence of vector ticks, weather conditions and smuggle animal across the border and they cause great damages to the economy and production. However, few studies have been done for differentiation of Theileria and Babesia in sheep in this area. The purpose of the present study was to determine the prevalence of Babesiosis and Theileriosis in sheep in Zabol...
December 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/27864774/comparison-of-an-automatic-analysis-and-a-manual-analysis-of-conjunctival-microcirculation-in-a-sheep-model-of-haemorrhagic-shock
#16
Philip-Helge Arnemann, Michael Hessler, Tim Kampmeier, Andrea Morelli, Hugo Karel Van Aken, Martin Westphal, Sebastian Rehberg, Christian Ertmer
BACKGROUND: Life-threatening diseases of critically ill patients are known to derange microcirculation. Automatic analysis of microcirculation would provide a bedside diagnostic tool for microcirculatory disorders and allow immediate therapeutic decisions based upon microcirculation analysis. METHODS: After induction of general anaesthesia and instrumentation for haemodynamic monitoring, haemorrhagic shock was induced in ten female sheep by stepwise blood withdrawal of 3 × 10 mL per kilogram body weight...
December 2016: Intensive Care Medicine Experimental
https://www.readbyqxmd.com/read/27863702/detecting-asymptomatic-rams-infected-with-mycoplasma-agalactiae-in-ovine-artificial-insemination-centers
#17
Miranda Prats-van der Ham, Juan Tatay-Dualde, Christian de la Fe, Ana Paterna, Antonio Sánchez, Juan Carlos Corrales, Antonio Contreras, Ángel Gómez-Martín
Mycoplasma agalactiae (Ma) is the main causative agent of ovine contagious agalactia, which is a serious disease of small ruminants. In endemic areas, its most common clinical situation consists of chronically infected herds, and asymptomatic infected individuals represent an epidemiological risk regarding the transmission of this disease. The aim of this work was to detect the presence of asymptomatic rams infected with Ma in different artificial insemination centers, and to determine the most effective way to identify these individuals so as to implement adequate surveillance protocols...
September 15, 2016: Theriogenology
https://www.readbyqxmd.com/read/27863622/objective-characterization-of-airway-dimensions-using-image-processing
#18
Victoria K Pepper, Christian Francom, Cameron A Best, Ekene Onwuka, Nakesha King, Eric Heuer, Nathan Mahler, Jonathan Grischkan, Christopher K Breuer, Tendy Chiang
OBJECTIVES: With the evolution of medical and surgical management for pediatric airway disorders, the development of easily translated techniques of measuring airway dimensions can improve the quantification of outcomes of these interventions. We have developed a technique that improves the ability to characterize endoscopic airway dimensions using common bronchoscopic equipment and an open-source image-processing platform. METHODS: We validated our technique of Endoscopic Airway Measurement (EAM) using optical instruments in simulation tracheas...
December 2016: International Journal of Pediatric Otorhinolaryngology
https://www.readbyqxmd.com/read/27860364/a-reproducible-method-for-the-isolation-and-expansion-of-ovine-mesenchymal-stromal-cells-from-bone-marrow-for-use-in-regenerative-medicine-preclinical-studies
#19
Marta Caminal, Roberto Vélez, Rosa Maria Rabanal, Daniel Vivas, Laura Batlle-Morera, Màrius Aguirre, Jordi Barquinero, Joan García, Joaquim Vives
The use of multipotent mesenchymal stromal cells (MSCs) as candidate medicines for treating a variety of pathologies is based on their qualities as either progenitors for the regeneration of damaged tissue or producers of a number of molecules with pharmacological properties. Preclinical product development programmes include the use of well characterized cell populations for proof of efficacy and safety studies before testing in humans. In the field of orthopaedics, an increasing number of translational studies use sheep as an in vivo test system because of the similarities with humans in size and musculoskeletal architecture...
November 18, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27858400/optimisation-of-bmp-2-dosage-for-the-osseointegration-of-porous-titanium-implants-in-an-ovine-model
#20
E B Hunziker, J Jovanovic, A Horner, M J Keel, K Lippuner, N Shintani
In clinical orthopaedics, total joint replacements and spinal fusions are routine undertakings. Many of the implicated patients suffer from osteoporosis, severe arthrosis or osteopaenia. In individuals thus afflicted, the bony bed lacks the mechanical stability that is a requisite for a firm anchorage of the implant and its functional competence. To promote the bony bondage of an implant it is necessary to induce neo-ossification by the introduction of an osteogenic agent, such as bone morphogenetic protein 2 (BMP-2)...
November 18, 2016: European Cells & Materials
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