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https://www.readbyqxmd.com/read/28346138/dilation-of-fusion-pores-by-crowding-of-snare-proteins
#1
Zhenyong Wu, Oscar D Bello, Sathish Thiyagarajan, Sarah Marie Auclair, Wensi Vennekate, Shyam S Krishnakumar, Ben O'Shaughnessy, Erdem Karatekin
Hormones and neurotransmitters are released through fluctuating exocytotic fusion pores that can flicker open and shut multiple times. Cargo release and vesicle recycling depend on the fate of the pore, which may reseal or dilate irreversibly. Pore nucleation requires zippering between vesicle-associated v- and target membrane t-SNAREs, but the mechanisms governing the subsequent pore dilation are not understood. Here, we probed dilation of single fusion pores using v-SNARE-reconstituted ~23 nm diameter discoidal nanolipoprotein particles (vNLPs) as fusion partners with cells ectopically expressing cognate, 'flipped' t-SNAREs...
March 27, 2017: ELife
https://www.readbyqxmd.com/read/28337782/three-dimensional-oxygen-enhanced-mri-of-the-human-lung-at-1-5t-with-ultra-fast-balanced-steady-state-free-precession
#2
Orso Pusterla, Grzegorz Bauman, Oliver Bieri
PURPOSE: To assess the feasibility of 3D oxygen-enhanced (OE) MRI of the lung at 1.5T using multi-volumetric ultra-fast balanced steady-state free precession (ufSSFP) acquisitions. METHODS: Isotropic imaging of the lung for OE-MRI was performed with an adapted 3D ufSSFP sequence using five breath-hold acquisitions ranging from functional residual capacity to tidal inspiration under both normoxic (room air) and hyperoxic (100% O2 ) gas conditions. For each O2 concentration, a sponge model (which captures the parenchymal signal intensity variation as a function of the lung volume) was fitted to the acquired multi-volumetric datasets after semiautomatic lung segmentation and deformable image registration...
March 24, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/28337119/analysis-of-the-precision-of-variable-flip-angle-t1-mapping-with-emphasis-on-the-noise-propagated-from-rf-transmit-field-maps
#3
Yoojin Lee, Martina F Callaghan, Zoltan Nagy
In magnetic resonance imaging, precise measurements of longitudinal relaxation time (T1) is crucial to acquire useful information that is applicable to numerous clinical and neuroscience applications. In this work, we investigated the precision of T1 relaxation time as measured using the variable flip angle method with emphasis on the noise propagated from radiofrequency transmit field ([Formula: see text]) measurements. The analytical solution for T1 precision was derived by standard error propagation methods incorporating the noise from the three input sources: two spoiled gradient echo (SPGR) images and a [Formula: see text] map...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/28334932/ribosome-dependent-vibrio-cholerae-mrnase-higb2-is-regulated-by-a-%C3%AE-strand-sliding-mechanism
#4
San Hadži, Abel Garcia-Pino, Sarah Haesaerts, Dukas Jurenas, Kenn Gerdes, Jurij Lah, Remy Loris
Toxin-antitoxin (TA) modules are small operons involved in bacterial stress response and persistence. higBA operons form a family of TA modules with an inverted gene organization and a toxin belonging to the RelE/ParE superfamily. Here, we present the crystal structures of chromosomally encoded Vibrio cholerae antitoxin (VcHigA2), toxin (VcHigB2) and their complex, which show significant differences in structure and mechanisms of function compared to the higBA module from plasmid Rts1, the defining member of the family...
February 28, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28332912/twelve-tips-for-the-production-of-digital-chalk-talk-videos
#5
Jasmine Rana, Henrike Besche, Barbara Cockrill
Increasingly over the past decade, faculty in medical and graduate schools have received requests from digital millennial learners for concise faculty-made educational videos. At our institution, over the past couple of years alone, several hundred educational videos have been created by faculty who teach in a flipped-classroom setting of the pre-clinical medical school curriculum. Despite the appeal and potential learning benefits of digital chalk-talk videos first popularized by Khan Academy, we have observed that the conceptual and technological barriers for creating chalk-talk videos can be high for faculty...
March 23, 2017: Medical Teacher
https://www.readbyqxmd.com/read/28330718/adult-glucocorticoid-receptor-mrna-expression-volatility-in-response-to-an-acute-stressor-and-juvenile-csf-corticotropin-releasing-factor-a-pilot-neurodevelopmental-study
#6
Shariful A Syed, Eric L P Smith, Olcay Batuman, Leonard A Rosenblum, Michael J Owens, Charles B Nemeroff, Jeremy D Coplan
INTRODUCTION: Early life stress (ELS) has been shown to play a role in establishing persistent maladaptive HPA axis modifications that may contribute to the pathogenesis of mood and anxiety disorders. Central glucocorticoid receptor (GR) messenger RNA (mRNA) expression may facilitate (mal)adaptive responsivity to ELS. The role of adult monocytic GR mRNA expression, a putative CNS proxy, during acute stress exposure was explored as well as the ELS marker, juvenile cerebrospinal fluid (CSF) corticotropin-releasing factor...
March 19, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28328770/the-flipped-classroom-benefits-of-student-led-learning
#7
Carolyn E Smith
No abstract text is available yet for this article.
April 2017: Nursing
https://www.readbyqxmd.com/read/28323722/flip-or-flop
#8
Lakshmi Mudambi, George A Eapen
No abstract text is available yet for this article.
April 2017: Journal of Bronchology & Interventional Pulmonology
https://www.readbyqxmd.com/read/28323377/accuracy-of-pdff-estimation-by-magnitude-based-and-complex-based-mri-in-children-with-mr-spectroscopy-as-a-reference
#9
William M Haufe, Tanya Wolfson, Catherine A Hooker, Jonathan C Hooker, Yesenia Covarrubias, Alex N Schlein, Gavin Hamilton, Michael S Middleton, Jorge E Angeles, Diego Hernando, Scott B Reeder, Jeffrey B Schwimmer, Claude B Sirlin
PURPOSE: To assess and compare the accuracy of magnitude-based magnetic resonance imaging (MRI-M) and complex-based MRI (MRI-C) for estimating hepatic proton density fat fraction (PDFF) in children, using MR spectroscopy (MRS) as the reference standard. A secondary aim was to assess the agreement between MRI-M and MRI-C. MATERIALS AND METHODS: This was a HIPAA-compliant, retrospective analysis of data collected in children enrolled in prospective, Institutional Review Board (IRB)-approved studies between 2012 and 2014...
March 21, 2017: Journal of Magnetic Resonance Imaging: JMRI
https://www.readbyqxmd.com/read/28321918/broadband-rf-amplitude-dependent-flip-angle-pulses-with-linear-phase-slope
#10
Martin R M Koos, Hannes Feyrer, Burkhard Luy
Pulse sequences in NMR spectroscopy sometimes require the application of pulses with effective flip angles different from 90˚ and 180˚ Previously (Magn. Reson. Chem.), offset-compensated broadband excitation pulses with rf-amplitude-dependent effective flip angles (RADFA) were introduced that are applicable in such cases. However, especially rf-amplitude-restricted RADFA pulses turned out to perform not as good as desired in terms of achievable bandwidths. Here, a class of rf-amplitude-restricted RADFA pulses with linear phase slope is introduced that allows excitation over much larger bandwidths with better performance...
March 20, 2017: Magnetic Resonance in Chemistry: MRC
https://www.readbyqxmd.com/read/28318229/tracing-effects-of-fluorine-substitutions-on-g-quadruplex-conformational-changes
#11
Jonathan Dickerhoff, Linn Haase, Walter Langel, Klaus Weisz
A human telomere sequence that folds into an intramolecular (3 + 1)-hybrid G-quadruplex was modified by the incorporation of 2'-fluoro-2'-deoxyriboguanosines ((F)G) into syn positions of its outer tetrad. A circular dichroism and NMR spectral analysis reveals a nearly quantitative switch of the G-tetrad polarity with concerted syn↔anti transitions of all four G residues. These observations follow findings on a (F)G-substituted (3 + 1)-hybrid quadruplex with a different fold, suggesting a more general propensity of hybrid-type quadruplexes to undergo a tetrad polarity reversal...
March 27, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28317964/how-does-ytterbium-chloride-interact-with-dmpc-bilayers-a-computational-and-experimental-study
#12
Miguel A Gonzalez, Hanna M G Barriga, Joanna L Richens, Robert V Law, Paul O'Shea, Fernando Bresme
Lanthanide salts have been studied for many years, primarily in Nuclear Magnetic Resonance (NMR) experiments of mixed lipid-protein systems and more recently to study lipid flip-flop in model membrane systems. It is well recognised that lanthanide salts can influence the behaviour of both lipid and protein systems, however a full molecular level description of lipid-lanthanide interactions is still outstanding. Here we present a study of lanthanide-bilayer interactions, using molecular dynamics computer simulations, fluorescence electrostatic potential experiments and nuclear magnetic resonance...
March 20, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28317908/determination-of-low-loss-in-isotopically-pure-single-crystal-28-si-at-low-temperatures-and-single-microwave-photon-energy
#13
Nikita Kostylev, Maxim Goryachev, Andrey D Bulanov, Vladimir A Gavva, Michael E Tobar
The low dielectric losses of an isotopically pure single crystal (28)Si sample were determined at a temperature of 20 mK and at powers equivalent to that of a single photon. Whispering Gallery Mode (WGM) analysis revealed large Quality Factors of order 2 × 10(6) (dielectric loss ~5 × 10(-7)) at high powers, degrading to 7 × 10(-5) (dielectric loss ~1.4 × 10(-6) at single photon energy. A very low-loss narrow line width paramagnetic spin flip transition was detected with extreme sensitivity in (28)Si, with very small concentration below 10(10) cm(-3) (less than 10 parts per trillion) and g-factor of 1...
March 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28315432/splicing-factors-of-sr-and-hnrnp-families-as-regulators-of-apoptosis-in-cancer
#14
Hanna Kędzierska, Agnieszka Piekiełko-Witkowska
SR and hnRNP proteins were initially discovered as regulators of alternative splicing: the process of controlled removal of introns and selective joining of exons through which multiple transcripts and, subsequently, proteins can be expressed from a single gene. Alternative splicing affects genes involved in all crucial cellular processes, including apoptosis. During cancerogenesis impaired apoptotic control facilitates survival of cells bearing molecular aberrations, contributing to their unrestricted proliferation and chemoresistance...
March 14, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28302032/anti-tumorigenic-effects-of-resveratrol-in-lung-cancer-cells-through-modulation-of-c-flip
#15
Clayton Wright, Anand Krishnan V Iyer, Juan S Yakisich, Neelam Azad
Resveratrol has been shown to have antioxidant and anti-proliferative properties in multiple cancer types. Here we demonstrate that H460 lung cancer cells are more susceptible to resveratrol treatment in comparison to human bronchial epithelial Beas-2B cells. Resveratrol decreases cell viability and proliferation, and induces significant apoptosis in H460 cells. The apoptosis observed was accompanied by an increase in hydrogen peroxide (H2O2) production, Bid, PARP and caspase 8 activation, and downregulation of pEGFR, pAkt, c-FLIP and NFkB protein expression...
March 15, 2017: Current Cancer Drug Targets
https://www.readbyqxmd.com/read/28301931/tunneling-effect-that-changes-the-reaction-pathway-from-epoxidation-to-hydroxylation-in-the-oxidation-of-cyclohexene-by-a-compound-i-model-of-cytochrome-p450
#16
Ranjana Gupta, Xiao-Xi Li, Kyung-Bin Cho, Mian Guo, Yong-Min Lee, Yong Wang, Shunichi Fukuzumi, Wonwoo Nam
The rate constants of the C=C epoxidation and the C-H hydroxylation (i.e., allylic C-H bond activation) in the oxidation of cyclohexene by a high-valent iron(IV)-oxo porphyrin π-cation radical complex, [(TMP(•+))Fe(IV)(O)(Cl)] (1, TMP = meso-tetramesitylporphyrin dianion), were determined at various temperatures by analyzing the overall rate constants and the products obtained in the cyclohexene oxidation by 1, leading us to conclude that the reaction pathway changes from the C=C epoxidation to the C-H hydroxylation by decreasing reaction temperature...
March 16, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/28301086/b1-mapping-for-bias-correction-in-quantitative-t1-imaging-of-the-brain-at-3t-using-standard-pulse-sequences
#17
Mathieu Boudreau, Christine L Tardif, Nikola Stikov, John G Sled, Wayne Lee, G Bruce Pike
PURPOSE: B1 mapping is important for many quantitative imaging protocols, particularly those that include whole-brain T1 mapping using the variable flip angle (VFA) technique. However, B1 mapping sequences are not typically available on many magnetic resonance imaging (MRI) scanners. The aim of this work was to demonstrate that B1 mapping implemented using standard scanner product pulse sequences can produce B1 (and VFA T1 ) maps comparable in quality and acquisition time to advanced techniques...
March 16, 2017: Journal of Magnetic Resonance Imaging: JMRI
https://www.readbyqxmd.com/read/28300412/reconciling-differences-between-lipid-transfer-in-free-standing-and-solid-supported-membranes-a-time-resolved-small-angle-neutron-scattering-study
#18
Benny Wah, Jeffrey Michael Breidigan, Joseph Adams, Piotr Horbal, Sumit Garg, Lionel Porcar, Ursula Perez-Salas
Maintaining compositional lipid gradients across membranes in animal cells is essential to biological function, but what is the energetic cost to maintain these differences? It has long been recognized that studying the passive movement of lipids in membranes can provide insight into this toll. Unfortunately the reported values of inter, and particularly, intra- lipid transport rates of lipids in membranes show significant differences. To overcome this difficulty, biases introduced by experimental approaches have to be identified...
March 16, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28297970/interplay-of-interfacial-noise-and-curvature-driven-dynamics-in-two-dimensions
#19
Parna Roy, Parongama Sen
We explore the effect of interplay of interfacial noise and curvature-driven dynamics in a binary spin system. An appropriate model is the generalized two-dimensional voter model proposed earlier [M. J. de Oliveira, J. F. F. Mendes, and M. A. Santos, J. Phys. A: Math. Gen. 26, 2317 (1993)JPHAC50305-447010.1088/0305-4470/26/10/006], where the flipping probability of a spin depends on the state of its neighbors and is given in terms of two parameters, x and y. x=0.5andy=1 correspond to the conventional voter model which is purely interfacial noise driven, while x=1 and y=1 correspond to the Ising model, where coarsening is fully curvature driven...
February 2017: Physical Review. E
https://www.readbyqxmd.com/read/28293202/automating-individualized-formative-feedback-in-large-classes-based-on-a-directed-concept-graph
#20
Henry E Schaffer, Karen R Young, Emily W Ligon, Diane D Chapman
Student learning outcomes within courses form the basis for course completion and time-to-graduation statistics, which are of great importance in education, particularly higher education. Budget pressures have led to large classes in which student-to-instructor interaction is very limited. Most of the current efforts to improve student progress in large classes, such as "learning analytics," (LA) focus on the aspects of student behavior that are found in the logs of Learning Management Systems (LMS), for example, frequency of signing in, time spent on each page, and grades...
2017: Frontiers in Psychology
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