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https://www.readbyqxmd.com/read/28420193/a-quantitative-pcr-electrochemical-genosensor-test-for-the-screening-of-biotech-crops
#1
Suely Moura-Melo, Rebeca Miranda-Castro, Noemí de-Los-Santos-Álvarez, Arturo J Miranda-Ordieres, José Ribeiro Dos Santos Junior, Rosana A da Silva Fonseca, María Jesús Lobo-Castañón
The design of screening methods for the detection of genetically modified organisms (GMOs) in food would improve the efficiency in their control. We report here a PCR amplification method combined with a sequence-specific electrochemical genosensor for the quantification of a DNA sequence characteristic of the 35S promoter derived from the cauliflower mosaic virus (CaMV). Specifically, we employ a genosensor constructed by chemisorption of a thiolated capture probe and p-aminothiophenol gold surfaces to entrap on the sensing layer the unpurified PCR amplicons, together with a signaling probe labeled with fluorescein...
April 18, 2017: Sensors
https://www.readbyqxmd.com/read/28417173/semiautomated-taqman-pcr-screening-of-gmo-labelled-samples-for-unauthorised-gmos
#2
Ingrid M J Scholtens, Bonnie Molenaar, Richard A van Hoof, Stephanie Zaaijer, Theo W Prins, Esther J Kok
In most countries, systems are in place to analyse food products for the potential presence of genetically modified organisms (GMOs), to enforce labelling requirements and to screen for the potential presence of unauthorised GMOs. With the growing number of GMOs on the world market, a larger diversity of methods is required for informative analyses. In this paper, the specificity of an extended screening set consisting of 32 screening methods to identify different crop species (endogenous genes) and GMO elements was verified against 59 different GMO reference materials...
April 17, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28414950/a-nanobiosensor-composed-of-exfoliated-graphene-oxide-and-gold-nano-urchins-for-detection-of-gmo-products
#3
Zahra Aghili, Navid Nasirizadeh, Adeleh Divsalar, Shahram Shoeibi, Parichehreh Yaghmaei
Genetically Modified Organisms, have been entered our food chain and detection of these organisms in market products are still the main challenge for scientists. Among several developed detection/quantification methods for detection of these organisms, the electrochemical nanobiosensors are the most attended which are combining the advantages of using nanomaterials, electrochemical methods and biosensors. In this research, a novel and sensitive electrochemical nanobiosensor for detection/quantification of these organisms have been developed using nanomaterials; Exfoliated Graphene Oxide and Gold Nano-Urchins for modification of the screen-printed carbon electrode, and also applying a specific DNA probe as well as hematoxylin for electrochemical indicator...
March 6, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28381773/a-novel-chemical-enhancer-approach-for-transdermal-drug-delivery-with-c17-monoglycerol-ester-liquid-crystal-forming-lipid
#4
Wesam R Kadhum, Shohei Sekiguchi, Ichiro Hijikuro, Hiroaki Todo, Kenji Sugibayashi
Transdermal administration of drugs represents an excellent alternative to conventional pharmaceutical dosage forms. However, insufficient penetration of the active pharmaceutical substance through the skin is a common problem. Thus, in the present study we evaluated the skin permeation enhancing ability of liquid crystal (LC) topical formulations. A recently developed LC-forming lipid, C17-monoglycerol ester (MGE), was evaluated and compared with glycerol monoolate (GMO), which is considered as the gold standard for LC formulations...
April 4, 2017: Journal of Oleo Science
https://www.readbyqxmd.com/read/28372191/a-temperature-tolerant-multiplex-elements-and-genes-screening-system-for-genetically-modified-organisms-based-on-dual-priming-oligonucleotide-primers-and-capillary-electrophoresis
#5
Wei Fu, Shuang Wei, Chenguang Wang, Zhixin Du, Pengyu Zhu, Xiyang Wu, Gang Wu, Shuifang Zhu
High throughput screening systems are the preferred solution to meet the urgent requirement of increasing number of genetically modified organisms (GMOs). In this study, we have successfully developed a multiplex GMO element screening system with dual priming oligonucleotide (DPO) primers. This system can detect the cauliflower mosaic virus 35S (CaMV 35S), terminator of nopaline synthase gene (NOS), figwort mosaic virus 35S (FMV 35S) promoter, neomycin phosphotransferaseII (NPTII), Bt Cry 1Ab, phosphinothricin acetyltransferase genes (bar) and Streptomyces viridochromogenes (pat) simultaneously, which covers more than 90% of all authorized GMO species worldwide...
August 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28362322/the-in-vitro-in-vivo-safety-confirmation-of-peg-40-hydrogenated-castor-oil-as-a-surfactant-for-oral-nanoemulsion-formulation
#6
Heni Rachmawati, Miranti Anggraeni Novel, Sri Ayu, Guntur Berlian, Olivia Mayasari Tandrasasmita, Raymond Rubianto Tjandrawinata, Kusnandar Anggadiredja
Evaluation on the safety use of high concentration of polyoxyl 40 (PEG-40) hydrogenated castor oil as a surfactant for oral nanoemulsion was performed in Webster mice. As previously reported, nearly 20% of PEG-40 hydrogenated castor oil was used to emulsify the glyceryl monooleate (GMO) as an oil to the aqueous phase. Thermodynamically stable and spontaneous nanoemulsion was formed by the presence of co-surfactant polyethylene glycol 400 (PEG-400). Standard parameters were analyzed for nanoemulsion including particle size and particle size distribution, the surface charge of nanoemulsion, and morphology...
March 31, 2017: Scientia Pharmaceutica
https://www.readbyqxmd.com/read/28347568/how-can-we-better-detect-unauthorized-gmos-in-food-and-feed-chains
#7
REVIEW
Marie-Alice Fraiture, Philippe Herman, Marc De Loose, Frédéric Debode, Nancy H Roosens
Current GMO detection systems have limited abilities to detect unauthorized genetically modified organisms (GMOs). Here, we propose a new workflow, based on next-generation sequencing (NGS) technology, to overcome this problem. In providing information about DNA sequences, this high-throughput workflow can distinguish authorized and unauthorized GMOs by strengthening the tools commonly used by enforcement laboratories with the help of NGS technology. In addition, thanks to its massive sequencing capacity, this workflow could be used to monitor GMOs present in the food and feed chain...
March 24, 2017: Trends in Biotechnology
https://www.readbyqxmd.com/read/28339113/preparation-and-oxidation-stability-evaluation-of-tea-polyphenols-loaded-inverse-micro-emulsion
#8
Xiaohong Lan, Jingjing Sun, Ying Yang, Mengjie Chen, Jianhua Liu, Jinhong Wu, Zhengwu Wang
Compared to synthetic antioxidants, tea polyphenols (TPs) has its own advantages in edible oil industry, however, the hydrophilic properties have restricted its applications. In this study, the ternary phase diagram of TPs-loaded micro-emulsion (ME) system was constructed, in which glyceryl monooleate (GMO), Tween80, linoleic acid as the surfactants, ethanol as the co-surfactant and soybean, corn, sunflower oil as the oil phase, have been used for the preparation of ME. The results indicated that a composition of ME (57...
March 24, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28331779/reply-to-the-efsa-2016-on-the-relevance-of-recent-publications-hofmann-et-al-2014-2016-on-environmental-risk-assessment-and-management-of-bt-maize-events-mon810-bt11-and-1507
#9
EDITORIAL
Maren Kruse-Plass, Frieder Hofmann, Ulrike Kuhn, Mathias Otto, Ulrich Schlechtriemen, Boris Schröder, Rudolf Vögel, Werner Wosniok
In this commentary, we respond to a report of the EFSA GMO Panel (EFSA EFSA Supp Publ, 1) that criticises the outcomes of two studies published in this journal (Hofmann et al. Environ Sci Eur 26: 24, 2; Environ Sci Eur 28: 14, 3). Both publications relate to the environmental risk assessment and management of Bt-maize, including maize events MON810, Bt11 and maize 1507. The results of Hofmann et al. (Environ Sci Eur 26: 24, 2), using standardised pollen mass filter deposition measurements, indicated that the EFSA Panel model had underestimated pollen deposition and, hence, exposure of non-target organisms to Bt-maize pollen...
2017: Environmental Sciences Europe
https://www.readbyqxmd.com/read/28324615/construction-of-low-ethanol-wine-yeasts-through-partial-deletion-of-the-saccharomyces-cerevisiae-pdc2-gene
#10
Raúl Andrés Cuello, Karina Johana Flores Montero, Laura Analía Mercado, Mariana Combina, Iván Francisco Ciklic
We propose an alternative GMO based strategy to obtain Saccharomyces cerevisiae mutant strains with a slight reduction in their ability to produce ethanol, but with a moderate impact on the yeast metabolism. Through homologous recombination, two truncated Pdc2p proteins Pdc2pΔ344 and Pdc2pΔ519 were obtained and transformed into haploid and diploid lab yeast strains. In the pdc2Δ344 mutants the DNA-binding and transactivation site of the protein remain intact, whereas in pdc2Δ519 only the DNA-binding site is conserved...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28316108/cry1a-b-16-toxin-from-bacillus-thuringiensis-theoretical-refinement-of-three-dimensional-structure-and-prediction-of-peptides-as-molecular-markers-for-detection-of-genetically-modified-organisms
#11
Alexandra Plácido, Andreia Coelho, Lucas Abreu Nascimento, Andreanne Gomes Vasconcelos, Maria Fátima Barroso, Joilson Ramos-Jesus, Vladimir Costa, Francisco das Chagas Alves Lima, Cristina Delerue-Matos, Ricardo Martins Ramos, Mariela M Marani, José Roberto de Souza de Almeida Leite
Transgenic maize produced by the insertion of the Cry transgene into its genome became the second most cultivated crop worldwide. Cry gene from Bacillus thuringiensis kurstaki expresses protein derivatives of crystalline endotoxins which confer insect resistance onto the maize crop. Mandatory labeling of processed food containing or made by genetically modified organisms is in force in many countries, so, it is very urgent to develop fast and practical methods for GMO identification, for example, biosensors...
March 18, 2017: Proteins
https://www.readbyqxmd.com/read/28278120/new-gmo-regulations-for-old-determining-a-new-future-for-eu-crop-biotechnology
#12
REVIEW
John Davison, Klaus Ammann
In this review, current EU GMO regulations are subjected to a point-by point analysis to determine their suitability for agriculture in modern Europe. Our analysis concerns present GMO regulations as well as suggestions for possible new regulations for genome editing and New Breeding Techniques (for which no regulations presently exist). Firstly, the present GMO regulations stem from the early days of recombinant DNA and are not adapted to current scientific understanding on this subject. Scientific understanding of GMOs has changed and these regulations are now, not only unfit for their original purpose, but, the purpose itself is now no longer scientifically valid...
January 2, 2017: GM Crops & Food
https://www.readbyqxmd.com/read/28254006/quantification-of-dna-fragmentation-in-processed-foods-using-real-time-pcr
#13
Junichi Mano, Yasuyuki Nishitsuji, Yosuke Kikuchi, Shin-Ichi Fukudome, Takuya Hayashida, Hiroyuki Kawakami, Youichi Kurimoto, Akio Noguchi, Kazunari Kondo, Reiko Teshima, Reona Takabatake, Kazumi Kitta
DNA analysis of processed foods is performed widely to detect various targets, such as genetically modified organisms (GMOs). Food processing often causes DNA fragmentation, which consequently affects the results of PCR analysis. In order to assess the effects of DNA fragmentation on the reliability of PCR analysis, we investigated a novel methodology to quantify the degree of DNA fragmentation. We designed four real-time PCR assays that amplified 18S ribosomal RNA gene sequences common to various plants at lengths of approximately 100, 200, 400, and 800 base pairs (bp)...
July 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28243885/effect-of-moisture-on-the-thermoresponsive-properties-of-binary-mixtures-of-monoglycerides-for-triggerable-drug-delivery-systems
#14
Hannah D Stonewall, Haley M Kessinger, Abebe E Mengesha
The crystallization behavior and temperature-dependent phase transition of monoglycerides have been utilized to develop thermal-sensitive drug delivery systems. The presence of excess water has been reported to influence the phase transition. The present study investigates the effect of moisture on the thermal behavior of binary blends of monoglycerides. Various compositions (0-100 wt%) of glyceryl monooleate (GMO) and glyceryl monostearate (GMS) were prepared by fusion method, and exposed to varying relative humidity (RH) levels (0-100%)...
February 27, 2017: AAPS PharmSciTech
https://www.readbyqxmd.com/read/28163712/seedusoon-a-new-software-program-to-improve-seed-stock-management-and-plant-line-exchanges-between-research-laboratories
#15
Céline Charavay, Stéphane Segard, Nathalie Pochon, Laurent Nussaume, Hélène Javot
Plant research is supported by an ever-growing collection of mutant or transgenic lines. In the past, a typical basic research laboratory would focus on only a few plant lines that were carefully isolated from collections of lines containing random mutations. The subsequent technological breakthrough in high-throughput sequencing, combined with novel and highly efficient mutagenesis techniques (including site-directed mutagenesis), has led to a recent exponential growth in plant line collections used by individual researchers...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28161965/pvx-tolerant-potato-development-using-a-nucleic-acid-hydrolyzing-recombinant-antibody
#16
J-G Yang, K-H Hwang, E-J Kil, J Park, S Cho, Y-G Lee, C-K Auh, Y Rhee, S Lee
3D8 scFv, a catalytic recombinant antibody developed in the MRL mouse, exhibits nucleic acid-hydrolyzing activity. Previous studies have demonstrated that tobacco plants harboring 3D8 scFv antibodies showed broad-spectrum resistance to infection by both DNA and RNA viruses. In this study, potatoes were transformed with the 3D8 scFv gene and screened by potato virus X (PVX) challenge. Starting with the T0 and T1 potato lines, PVX-tolerant T1 potatoes were identified in the field and characterized by ELISA and RT-PCR analysis...
February 6, 2017: Acta Virologica
https://www.readbyqxmd.com/read/28154881/multiplex-enrichment-quantitative-pcr-me-qpcr-a-high-throughput-highly-sensitive-detection-method-for-gmo-identification
#17
Wei Fu, Pengyu Zhu, Shuang Wei, Du Zhixin, Chenguang Wang, Xiyang Wu, Feiwu Li, Shuifang Zhu
Among all of the high-throughput detection methods, PCR-based methodologies are regarded as the most cost-efficient and feasible methodologies compared with the next-generation sequencing or ChIP-based methods. However, the PCR-based methods can only achieve multiplex detection up to 15-plex due to limitations imposed by the multiplex primer interactions. The detection throughput cannot meet the demands of high-throughput detection, such as SNP or gene expression analysis. Therefore, in our study, we have developed a new high-throughput PCR-based detection method, multiplex enrichment quantitative PCR (ME-qPCR), which is a combination of qPCR and nested PCR...
February 2, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28123987/bioadhesive-floating-microsponges-of-cinnarizine-as-novel-gastroretentive-delivery-capmul-gmo-bioadhesive-coating-versus-acconon-mc-8-2-ep-nf-with-intrinsic-bioadhesive-property
#18
Smita Raghuvanshi, Kamla Pathak
INTRODUCTION: The study was aimed at the development of low-density gastroretentive bioadhesive microsponges of cinnarizine by two-pronged approach (i) coating with bioadhesive material and (ii) exploration of acconon MC 8-2 EP/NF as bioadhesive raw material for fabrication. MATERIALS AND METHODS: Microsponges were prepared by quasi-emulsion solvent diffusion method using 3(2) factorial design. Capmul GMO was employed for bioadhesive coating. In parallel, potential of acconon for the fabrication of bioadhesive floating microsponges (A8) was assessed...
October 2016: International Journal of Pharmaceutical Investigation
https://www.readbyqxmd.com/read/28092385/visual-detection-of-multiple-genetically-modified-organisms-in-a-capillary-array
#19
Ning Shao, Jianwei Chen, Jiaying Hu, Rong Li, Dabing Zhang, Shujuan Guo, Junhou Hui, Peng Liu, Litao Yang, Sheng-Ce Tao
There is an urgent need for rapid, low-cost multiplex methodologies for the monitoring of genetically modified organisms (GMOs). Here, we report a C[combining low line]apillary A[combining low line]rray-based L[combining low line]oop-mediated isothermal amplification for M[combining low line]ultiplex visual detection of nucleic acids (CALM) platform for the simple and rapid monitoring of GMOs. In CALM, loop-mediated isothermal amplification (LAMP) primer sets are pre-fixed to the inner surface of capillaries...
January 16, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28071020/the-effect-of-microwave-roasting-on-the-antioxidant-properties-of-the-bangladeshi-groundnut-cultivar
#20
Abbas Ali, Anowarul Islam, Tarun K Pal
BACKGROUND: Groundnut seeds are an important source of bioactive phenolic compounds with noteworthy antioxidant capacity, which may be enhanced by the microwave roasting process. The aim of this work is   to study the changes in antioxidant activity in groundnut seeds during microwave roasting, as a function of roasting time and extract concentration, in order to maximise the phenolic content and antioxidant activity of roasted seeds. METHODS: The study was conducted to evaluate total phenolic content (TPC), total flavonoid content (TFC), and antioxidative activity of methanolic (GME), ethanolic (GEE), and chloroform (GCE) extracts and methanolic extract of oil (GMO) from groundnut seeds exposed to microwaves...
October 2016: Acta Scientiarum Polonorum. Technologia Alimentaria
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