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https://www.readbyqxmd.com/read/29319683/why-the-european-union-needs-a-national-gmo-opt-in-mechanism
#1
Dennis Eriksson, Eugénia de Andrade, Borut Bohanec, Sevasti Chatzopolou, Roberto Defez, Nélida Leiva Eriksson, Piet van der Meer, Bernd van der Meulen, Anneli Ritala, László Sági, Joachim Schiemann, Tomasz Twardowski, Tomáš Vaněk
No abstract text is available yet for this article.
January 10, 2018: Nature Biotechnology
https://www.readbyqxmd.com/read/29286383/visual-detection-of-multiple-nucleic-acids-in-a-capillary-array
#2
Jianwei Chen, Ning Shao, Jiaying Hu, Rong Li, Yuanshou Zhu, Dabing Zhang, Shujuan Guo, Junhou Hui, Peng Liu, Litao Yang, Sheng-Ce Tao
Multi-target, short time, and resource-affordable methodologies for the detection of multiple nucleic acids in a single, easy to operate test are urgently needed in disease diagnosis, microbial monitoring, genetically modified organism (GMO) detection, and forensic analysis. We have previously described the platform called CALM (Capillary Array-based Loop-mediated isothermal amplification for Multiplex visual detection of nucleic acids). Herein, we describe improved fabrication and performance processes for this platform...
November 15, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29243104/skepticism-regarding-vaccine-and-gluten-free-food-safety-among-patients-with-celiac-disease-and-non-celiac-gluten-sensitivity
#3
Loren G Rabinowitz, Haley M Zylberberg, Alan Levinovitz, Melissa S Stockwell, Peter H R Green, Benjamin Lebwohl
BACKGROUND: There has been a marked increase in the adoption of the gluten-free (GF) diet. AIMS: To query individuals with celiac disease (CD) and non-celiac gluten sensitivity (NCGS) on their beliefs toward the health effects of gluten, and safety of vaccines and GF food products. METHODS: We distributed a Web-based survey to individuals with CD and NCGS on a CD center e-mail list. We used univariate and multivariate analysis to compare responses of respondents with CD and NCGS...
December 14, 2017: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/29236423/gmos-genetically-modified-organisms-or-genuinely-mixed-opinions-a-reasonable-consumer%C3%A2-s-understanding-of-the-terms-gmo-and-non-gmo-and-the-struggle-to-set-a-standard-%C3%AF-%C2%AA
#4
https://www.readbyqxmd.com/read/29173000/the-central-dogma-gmo-and-defective-epistemology
#5
Giovanni Tagliabue
The expression "Genetically Modified Organisms" was coined to indicate a group of agricultural products (mostly crops and vegetables), modified through direct DNA recombination in order to obtain useful phenotypic traits or to inhibit undesirable characteristics. But the border between rDNA ("GMO") and other biotech methods is blurred. Moreover, the ill-assorted group is frequently charged with having peculiar, negative characteristics: many activists, part of the public and a few social science scholars think that "GMOs" are all dubious, even inherently dangerous...
November 27, 2017: GM Crops & Food
https://www.readbyqxmd.com/read/29147107/debated-agronomy-public-discourse-and-the-future-of-biotechnology-policy-in-ghana
#6
Joseph A Braimah, Kilian N Atuoye, Siera Vercillo, Carrie Warring, Isaac Luginaah
This paper examines the highly contested and ongoing biotechnology (Bt) policy-making process in Ghana. We analyse media content on how Bt is viewed in the context of Ghana's parliamentary debate on the Plant Breeders Bill and within the broader public policy-making literature. This paper does not seek to take a position on Bt or the Bill, but to understand how policy actors influence the debate with political and scientific rhetoric in Ghana. The study reveals that in the midst of scientific uncertainties of Bt's potential for sustainable agriculture production and food security, policy decisions that encourage its future adoption are heavily influenced by health, scientific, economic, environmental and political factors dictated by different ideologies, values and norms...
2017: Global Bioethics, Problemi di Bioetica
https://www.readbyqxmd.com/read/29130156/crispr-from-prokaryotic-immune-systems-to-plant-genome-editing-tools
#7
Anindya Bandyopadhyay, Shamik Mazumdar, Xiaojia Yin, William Paul Quick
The clustered regularly interspaced short palindromic repeats (CRISPR) system is a prokaryotic adaptive immune system that has the ability to identify specific locations on the bacteriophage (phage) genome to create breaks in it, and internalize the phage genome fragments in its own genome as CRISPR arrays for memory-dependent resistance. Although CRISPR has been used in the dairy industry for a long time, it recently gained importance in the field of genome editing because of its ability to precisely target locations in a genome...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29110175/optimization-and-verification-of-droplet-digital-pcr-even-specific-methods-for-the-quantification-of-gm-maize-das1507-and-nk603
#8
Katarzyna Grelewska-Nowotko, Magdalena Żurawska-Zajfert, Ewelina Żmijewska, Sławomir Sowa
In recent years, digital polymerase chain reaction (dPCR), a new molecular biology technique, has been gaining in popularity. Among many other applications, this technique can also be used for the detection and quantification of genetically modified organisms (GMOs) in food and feed. It might replace the currently widely used real-time PCR method (qPCR), by overcoming problems related to the PCR inhibition and the requirement of certified reference materials to be used as a calibrant. In theory, validated qPCR methods can be easily transferred to the dPCR platform...
November 6, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/29108729/-liquid-crystalline-systems-based-on-glyceryl-monooleate-and-penetration-enhancers-for-skin-delivery-of-celecoxib-characterization-in-vitro-drug-release-and-in-vivo-studies
#9
Mariane de Cássia Lima Dante, Livia Neves Borgheti-Cardoso, Marcia Carvalho de Abreu Fantini, Fabíola Silva Garcia Praça, Wanessa Silva Garcia Medina, Maria Bernadete Riemma Pierre, Marilisa Guimarães Lara
Celecoxib (CXB) is a widely used anti-inflammatory drug that also acts as a chemopreventive agent against several types of cancer, including skin cancer. As the long-term oral administration of CXB has been associated with severe side effects, the skin delivery of this drug represents a promising alternative for the treatment of skin inflammatory conditions and/or chemoprevention of skin cancer. We prepared and characterized liquid crystalline systems based on glyceryl monooleate (GMO) and water containing penetration enhancers which were primarily designed to promote skin delivery of CXB...
November 3, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29105251/isolation-of-five-rice-non-endosperm-tissue-expressed-promoters-and-evaluation-of-their-activities-in-transgenic-rice
#10
Hao Li, Juan Li, Rongfang Xu, Ruiying Qin, Fengshun Song, Li Li, Pengcheng Wei, Jianbo Yang
Using promoters expressed in non-endosperm tissues to activate target genes in specific plant tissues or organs with very limited expression in the endosperm is an attractive approach in crop transgenic engineering. In this paper, five putative non-endosperm tissue expressed promoters were cloned from the rice genome and designated POsNETE1 , POsNETE2 , POsNETE3 , POsNETE4 and POsNETE5 . By qualitatively and quantitatively examining GUSplus reporter gene expression in transgenic rice plants, POsNETE1 -POsNETE5 were all found to be active in the roots, leaves, stems, sheaths and panicles but not in the endosperm of plants at different developmental stages...
November 3, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29074984/alf-a-strategy-for-identification-of-unauthorized-gmos-in-complex-mixtures-by-a-gw-ngs-method-and-dedicated-bioinformatics-analysis
#11
Alexandra Bogožalec Košir, Alfred J Arulandhu, Marleen M Voorhuijzen, Hongmei Xiao, Rico Hagelaar, Martijn Staats, Adalberto Costessi, Jana Žel, Esther J Kok, Jeroen P van Dijk
The majority of feed products in industrialised countries contains materials derived from genetically modified organisms (GMOs). In parallel, the number of reports of unauthorised GMOs (UGMOs) is gradually increasing. There is a lack of specific detection methods for UGMOs, due to the absence of detailed sequence information and reference materials. In this research, an adapted genome walking approach was developed, called ALF: Amplification of Linearly-enriched Fragments. Coupling of ALF to NGS aims for simultaneous detection and identification of all GMOs, including UGMOs, in one sample, in a single analysis...
October 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29071363/inter-laboratory-analysis-of-selected-genetically-modified-plant-reference-materials-with-digital-pcr
#12
David Dobnik, Tina Demšar, Ingrid Huber, Lars Gerdes, Sylvia Broeders, Nancy Roosens, Frederic Debode, Gilbert Berben, Jana Žel
Digital PCR (dPCR), as a new technology in the field of genetically modified (GM) organism (GMO) testing, enables determination of absolute target copy numbers. The purpose of our study was to test the transferability of methods designed for quantitative PCR (qPCR) to dPCR and to carry out an inter-laboratory comparison of the performance of two different dPCR platforms when determining the absolute GM copy numbers and GM copy number ratio in reference materials certified for GM content in mass fraction. Overall results in terms of measured GM% were within acceptable variation limits for both tested dPCR systems...
October 25, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29068685/development-of-monoclonal-antibodies-recognizing-linear-epitope-illustration-by-three-bacillus-thuringiensis-crystal-proteins-of-genetically-modified-cotton-maize-and-tobacco
#13
Zhen Cao, Wei Zhang, Xiangxue Ning, Baomin Wang, Yunjun Liu, Qing X Li
Bacillus thuringiensis Cry1Ac, Cry1Ia1, and Cry1Ie are δ-endotoxin insecticidal proteins widely implemented in genetically modified organisms (GMO), such as cotton, maize, and potato. Western blot assay integrates electrophoresis separation power and antibody high specificity for monitoring specific exogenous proteins expressed in GMO. Procedures for evoking monoclonal antibody (mAb) for Western blot were poorly documented. In the present study, Cry1Ac partially denatured at 100 °C for 5 min was used as an immunogen to develop mAbs selectively recognizing a linear epitope of Cry1Ac for Western blot...
November 9, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29041813/regulatory-science-requirements-of-labeling-of-genetically-modified-food
#14
A Alan Moghissi, Lisa M Jaeger, Dania Shafei, Lindsey L Bloom
This paper provides an overview of the evolution of food labeling in the USA. It briefly describes the three phases of agricultural development consisting of naturally occurring, cross-bred, and genetically engineered, edited or modified crops, otherwise known as Genetically Modified Organisms (GMO). It uses the Best Available Regulatory Science (BARS) and Metrics for Evaluation of Regulatory Science Claims (MERSC) to evaluate the scientific validity of claims applicable to GMO and the Best Available Public Information (BAPI) to evaluate the pronouncements by public media and others...
October 17, 2017: Critical Reviews in Biotechnology
https://www.readbyqxmd.com/read/29027137/cubic-liquid-crystalline-gels-based-on-glycerol-monooleate-for-intra-articular-injection
#15
Qian Li, Jiaojiao Cao, Zhengguang Li, Xiaoqin Chu
In situ gels containing sinomenine hydrochloride (SMH) for intra-articular (IA) administration to treat rheumatoid arthritis (RA) were designed and investigated in this study. Glycerol monooleate (GMO) was used due to the potential to generate viscous crystalline phase structures upon water absorption. The gels were evaluated using different parameters: syringeability, gelation, viscosity, and drug release. And, polarized light microscopy (PLM), small-angle X-ray scattering investigation (SAXS), and rheological studies were used to analyze their internal structures...
October 12, 2017: AAPS PharmSciTech
https://www.readbyqxmd.com/read/28993590/an-electrochemical-dna-biosensor-based-on-au-reduced-graphene-oxide-nanocomposite-for-transgenic-event-bt63-detection
#16
Chenmeng He, Yali Tang, Sai Wang, Jiahui Liu, Ying Chen, Yiyang Dong, Haijia Su, Tianwei Tan
A simple and reliable electrochemical biosensor based on an in situ synthetic Au-reduced graphene oxide (Au-RGO) nanocomposite was constructed in this work, which is considered to be a potential sensing platform for sensitive and selective Bt63 rice detection. UV-vis spectroscopy, transmission electron microscopy (TEM), and cyclic voltammograms of the surface characterization indicated that RGO was prepared successfully and Au nanoparticles were well dispersed on its surface with an average size of 20 nm. The square-wave voltammetry (SWV) response of the electrochemical marker methylene blue (MB) was chosen to monitor the probe immobilization and target hybridization event...
2017: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/28929711/-statistics-at-the-time-of-the-crisis
#17
Andrea Saltelli, Philip B Stark
Science lies nowadays in the centre of several storms. The better known is the finding of non-reproducibility of many scientific results, which stretches from the medical field (clinic and pre-clinic tests) to study on behaviour (priming research). Although the bad use of statistics is reported to be a patent cause of the reproducibility crisis, its deep reasons are to be sought elsewhere; particularly, in the passage from a regimen of little science - regulated by small communities of researchers - to the current big science - identified by a hypertrophic production of millions of research papers and by the imperative "publish or perish", in a setting dominated by market...
May 2017: Epidemiologia e Prevenzione
https://www.readbyqxmd.com/read/28924152/second-harmonic-generation-response-in-thermally-reconstructed-multiferroic-%C3%AE-gd2-moo4-3-thin-films
#18
Emerson Coy, Piotr Graczyk, Luis Yate, Karol Załęski, Jacek Gapiński, Piotr Kuświk, Sławomir Mielcarek, Feliks Stobiecki, Bogusław Mróz, Cesar Ferrater, Stefan Jurga
Gd2(MoO4)3 (GMO) is a well-studied multiferroic material that exhibits full ferroelectric and ferroelastic behavior at room temperature. However, its difficult stabilization in thin films has prevented the study and exploitation of its multiferroic properties in different architectures. Here, we report on the study of GMO thin films deposited on Si(001) substrates by Pulsed Laser Deposition (PLD). The physicochemical properties of the films are discussed and studied. Results obtained by X-ray diffraction, X-ray photoelectron spectroscopy, high resolution transmission microscopy and second harmonic generation show that the orthorhombic (β'-GMO) multiferroic phase can be stabilized and homogenized by post deposition thermal reconstruction...
September 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28866460/nitric-oxide-sensing-actuators-for-modulating-structure-in-lipid-based-liquid-crystalline-drug-delivery-systems
#19
Qingtao Liu, Jinming Hu, Michael R Whittaker, Thomas P Davis, Ben J Boyd
Herein we report on the development of a nitric oxide-sensing lipid-based liquid crystalline (LLC) system specifically designed to release encapsulated drugs on exposure to NO through a stimulated phase change. A series of nitric oxide (NO)-sensing lipids compatible with phytantriol and GMO cubic phases were designed and synthesized, and utilized in enabling nitric oxide-sensing LLC systems. The nitric oxide (NO)-sensing lipids react with nitric oxide, resulting in hydrolysis of these lipids and phase transition of the LLC system...
August 24, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28859885/case-studies-on-genetically-modified-organisms-gmos-potential-risk-scenarios-and-associated-health-indicators
#20
Barbara De Santis, Norbert Stockhofe, Jean-Michel Wal, Eefke Weesendorp, Jean-Paul Lallès, Jeroen van Dijk, Esther Kok, Marzia De Giacomo, Ralf Einspanier, Roberta Onori, Carlo Brera, Paul Bikker, Jan van der Meulen, Kleter Gijs
Within the frame of the EU-funded MARLON project, background data were reviewed to explore the possibility of measuring health indicators during post-market monitoring for potential effects of feeds, particularly genetically modified (GM) feeds, on livestock animal health, if applicable. Four case studies (CSs) of potential health effects on livestock were framed and the current knowledge of a possible effect of GM feed was reviewed. Concerning allergenicity (CS-1), there are no case-reports of allergic reactions or immunotoxic effects resulting from GM feed consumption as compared with non-GM feed...
August 30, 2017: Food and Chemical Toxicology
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