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https://www.readbyqxmd.com/read/29728956/the-assessment-of-field-trials-in-gmo-research-around-the-world-and-their-possible-integration-in-field-trials-for-variety-registration
#1
REVIEW
M M Slot, C C M van de Wiel, G A Kleter, R G F Visser, E J Kok
Most regulations worldwide stipulate that a new genetically modified (GM) crop event has to be compared to its closest non-GM counterpart as a corner stone of the pre-market risk assessment. To this end the GM crop and its comparator should be grown in field trials for a phenotypic comparison as well as for subsequent detailed analysis of the composition of the two crop varieties. A more in-depth globally harmonised approach for the conduct of these field trials is lacking. Only a few countries have formulated detailed protocols for the set-up of GM field trials...
May 4, 2018: Transgenic Research
https://www.readbyqxmd.com/read/29690627/what-is-natural-consumer-responses-to-selected-ingredients
#2
Edgar Chambers, Edgar Chambers, Mauricio Castro
Interest in “natural” food has grown enormously over the last decade. Because the United States government has not set a legal definition for the term “natural”, customers have formed their own sensory perceptions and opinions on what constitutes natural. In this study, we examined 20 ingredients to determine what consumers consider to be natural. Using a national database, 630 consumers were sampled (50% male and 50% female) online, and the results were analyzed using percentages and chi-square tests...
April 23, 2018: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/29680759/impact-of-genetically-modified-organisms-on-aquatic-environments-review-of-available-data-for-the-risk-assessment
#3
REVIEW
Antonia Pott, Mathias Otto, Ralf Schulz
The aquatic environment is strongly connected to the surrounding agricultural landscapes, which regularly serve as sources of stressors such as agrochemicals. Genetically modified crops, which are cultivated on a large scale in many countries, may also act as stressors. Despite the commercial use of genetically modified organisms (GMOs) for over 20years, their impact on the aquatic environment came into focus only 10years ago. We present the status quo of the available scientific data in order to provide an input for informed aquatic risk assessment of GMOs...
April 19, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29678222/loop-mediated-isothermal-amplification-for-detection-of-endogenous-sad1-gene-in-cotton-an-internal-control-for-rapid-onsite-gmo-testing
#4
Monika Singh, Rajesh K Bhoge, Gurinderjit Randhawa
Background : Confirming the integrity of seed samples in powdered form is important priorto conducting a genetically modified organism (GMO) test. Rapid onsite methods may provide a technological solution to check for genetically modified (GM) events at ports of entry. In India, Bt cotton is the commercialized GM crop with four approved GM events; however, 59 GM events have been approved globally. GMO screening is required to test for authorized GM events. The identity and amplifiability of test samples could be ensured first by employing endogenous genes as an internal control...
April 20, 2018: Journal of AOAC International
https://www.readbyqxmd.com/read/29652133/ultra-sensitive-single-fluorescence-labeled-probe-mediated-sup-m-ddpcr-for-high-throughput-gmo-screening
#5
Chenqi Niu, Yuancong Xu, Chao Zhang, Pengyu Zhu, Kunlun Huang, Yunbo Luo, Wentao Xu
As genetically modified (GM) technology develops and genetically modified organisms (GMOs) become more available, GMOs face increasing regulations and pressure to adhere to strict labeling guidelines. A singleplex detection method cannot perform the high-throughput analysis necessary for optimal GMO detection. Combining the advantages of multiplex detection and droplet digital polymerase chain reaction (ddPCR), a single universal primer-multiplex-ddPCR (SUP-M-ddPCR) strategy was proposed for accurate broad-spectrum screening and quantification...
April 13, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29629662/vitamin-e-tpgs-emulsified-vinorelbine-bitartrate-loaded-solid-lipid-nanoparticles-sln-formulation-development-optimization-and-in-vitro-characterization
#6
Lakshmi Maurya, Vijayakumar Mahalingam Rajamanickam, Gopeshwar Narayan, Sanjay Singh
BACKGROUND: Vinorelbine bitartrate (VRL), a semi synthetic vinca alkaloid approved for breast cancer, has been proved to beneficial as first line and subsequent therapies. However, it's hydrophilic and thermo labile nature provides hindrance to oral clinical translation. OBJECTIVES: The current work focused on the application of DOE a modern statistical optimization tool for the development and optimization of a solid lipid nanoparticle (SLN) formulation that can encapsulate hydrophilic and thermolabile Vinorelbine bitartrate (VRL) to a maximum extent without compromising integrity and anticancer activity of the drug...
April 8, 2018: Current Drug Delivery
https://www.readbyqxmd.com/read/29609161/electrochemical-genoassays-on-gold-coated-magnetic-nanoparticles-to-quantify-genetically-modified-organisms-gmos-in-food-and-feed-as-gmo-percentage
#7
Alexandra Plácido, Clara Pereira, Alexandra Guedes, M Fátima Barroso, Rebeca Miranda-Castro, Noemí de-Los-Santos-Álvarez, Cristina Delerue-Matos
The integration of nanomaterials in the field of (bio)sensors has allowed developing strategies with improved analytical performance. In this work, ultrasmall core-shell Fe3 O4 @Au magnetic nanoparticles (MNPs) were used as the platform for the immobilization of event-specific Roundup Ready (RR) soybean and taxon-specific DNA sequences. Firstly, monodisperse Fe3 O4 MNPs were synthesized by thermal decomposition and subsequently coated with a gold shell through reduction of Au(III) precursor on the surface of the MNPs in the presence of an organic capping agent...
March 21, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29603901/behavior-of-several-pseudomonas-putida-strains-growth-under-different-agitation-and-oxygen-supply-conditions
#8
Alberto Rodriguez, Sebastian Escobar, Emilio Gomez, Victoria E Santos, Felix Garcia-Ochoa
The growth rate of four strains of Pseudomonas putida, KT2440, KT2442, KTH2 and KTH2 (pESOX3), under different fluid dynamic conditions has been studied. The cultures were conducted in a stirred tank bioreactor by changing the stirrer speed. Several process variables, such as biomass concentration, dissolved oxygen concentration, oxygen mass transfer rate and oxygen uptake rate, have been measured or calculated. Also cell viability was determined by viable colony counting in Petri dishes and culture samples were subjected into a transmission electron microscopy analysis, in order to describe the integrity of the individual cells...
March 31, 2018: Biotechnology Progress
https://www.readbyqxmd.com/read/29602252/the-swedish-policy-approach-to-directed-mutagenesis-in-a-european-context
#9
Dennis Eriksson
This review describes the Swedish approach to directed mutagenesis in plants and puts it in a comparative European perspective. Directed mutagenesis is accomplished by a number of genome editing techniques, however the legal status of these techniques and their resulting products is uncertain in the European Union (EU) as there is no political consensus on whether or not these should be regulated as genetically modified organisms (GMO). A number of cases have developed over the past few years, putting the GMO regulatory framework to the test...
March 30, 2018: Physiologia Plantarum
https://www.readbyqxmd.com/read/29594619/colorimetric-detection-of-genetically-modified-organisms-based-on-exonuclease-iii-assisted-target-recycling-and-hemin-g-quadruplex-dnazyme-amplification
#10
Decai Zhang, Weijia Wang, Qian Dong, Yunxiu Huang, Dongmei Wen, Yuejing Mu, Yong Yuan
An isothermal colorimetric method is described for amplified detection of the CaMV 35S promoter sequence in genetically modified organism (GMO). It is based on (a) target DNA-triggered unlabeled molecular beacon (UMB) termini binding, and (b) exonuclease III (Exo III)-assisted target recycling, and (c) hemin/G-quadruplex (DNAzyme) based signal amplification. The specific binding of target to the G-quadruplex sequence-locked UMB triggers the digestion of Exo III. This, in turn, releases an active G-quadruplex segment and target DNA for successive hybridization and cleavage...
December 21, 2017: Mikrochimica Acta
https://www.readbyqxmd.com/read/29584422/development-of-certified-matrix-based-reference-material-as-calibrator-for-genetically-modified-rice-g6h1-analysis
#11
Yu Yang, Liang Li, Hui Yang, Xiaying Li, Xiujie Zhang, Junfeng Xu, Dabing Zhang, Wujun Jin, Litao Yang
The accurate monitoring and quantification of genetically modified organisms (GMOs) are key points for the implementation of labelling regulations, and a certified reference material (CRM) acts as the scaleplate for quantifying the GM contents of foods/feeds and evaluating a GMO analytical method or equipment. Herein, we developed a series of CRMs for transgenic rice event G6H1, which possesses insect-resistant and herbicide-tolerant traits. Three G6H1 CRMs were produced by mixing seed powders obtained from homozygous G6H1 and its recipient cultivar Xiushui 110 at mass ratios of 49...
March 27, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29580925/macbeth-multiplex-automated-corynebacterium-glutamicum-base-editing-method
#12
Yu Wang, Ye Liu, Jiao Liu, Yanmei Guo, Liwen Fan, Xiaomeng Ni, Xiaomei Zheng, Meng Wang, Ping Zheng, Jibin Sun, Yanhe Ma
CRISPR/Cas9 or Cpf1-introduced double strand break dramatically decreases bacterial cell survival rate, which hampers multiplex genome editing in bacteria. In addition, the requirement of a foreign DNA template for each target locus is labor demanding and may encounter more GMO related regulatory hurdle in industrial applications. Herein, we developed a multiplex automated Corynebacterium glutamicum base editing method (MACBETH) using CRISPR/Cas9 and activation-induced cytidine deaminase (AID), without foreign DNA templates, achieving single-, double-, and triple-locus editing with efficiencies up to 100%, 87...
March 24, 2018: Metabolic Engineering
https://www.readbyqxmd.com/read/29574561/critical-assessment-of-digital-pcr-for-the-detection-and-quantification-of-genetically-modified-organisms
#13
REVIEW
Tigst Demeke, David Dobnik
The number of genetically modified organisms (GMOs) on the market is steadily increasing. Because of regulation of cultivation and trade of GMOs in several countries, there is pressure for their accurate detection and quantification. Today, DNA-based approaches are more popular for this purpose than protein-based methods, and real-time quantitative PCR (qPCR) is still the gold standard in GMO analytics. However, digital PCR (dPCR) offers several advantages over qPCR, making this new technique appealing also for GMO analysis...
March 24, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29565638/biosensors-for-gmo-testing-nearly-25-years-of-research
#14
Marta Sánchez-Paniagua López, Carmen Lorena Manzanares-Palenzuela, Beatriz López-Ruiz
In the nearly two decades since genetically modified organisms (GMOs) were first commercialized, genetically engineered crops have gained ground on their conventional counterparts, reaching 185 million hectares worldwide in 2016. The technology has bestowed most of its benefits on enhancing crop productivity with two main traits currently dominating the market: insect-resistant and herbicide-tolerant crops. Despite their rapid and vast adoption by farmers worldwide, GMOs have generated heated debates, especially in European countries (EU), driven mostly by consumers concerned about safety of transgenic foods and about the potential impact on the environment...
March 22, 2018: Critical Reviews in Analytical Chemistry
https://www.readbyqxmd.com/read/29561212/sowing-the-seeds-of-skepticism-russian-state-news-and-anti-gmo-sentiment
#15
Shawn F Dorius, Carolyn J Lawrence-Dill
Biotech news coverage in English-language Russian media fits the profile of the Russian information warfare strategy described in recent military reports. This raises the question of whether Russia views the dissemination of anti-GMO information as just one of many divisive issues it can exploit as part of its information war, or if GMOs serve more expansive disruptive purposes. Distinctive patterns in Russian news provide evidence of a coordinated information campaign that could turn public opinion against genetic engineering...
March 21, 2018: GM Crops & Food
https://www.readbyqxmd.com/read/29540978/threshold-level-and-traceability-of-roundup-ready%C3%A2-soybeans-in-tofu-production
#16
Zorica Nikolić, Gordana Petrović, Dejana Panković, Maja Ignjatov, Dragana Marinković, Milan Stojanović, Vuk Đorđević
The aim of this study is to assess DNA degradation, DNA amplification, and GMO quantity during tofu production. Soybean seeds were spiked with Roundup Ready® soybeans (RRS) at 0.9, 2, 3 and 5% (by mass), to assess the level of RSS that would be of practical interest for threshold labelling. Real-time polymerase chain reaction (PCR) was more effective than conventional PCR in the analysis of raw soymilk, okara, boiled soymilk and tofu. The negative effect of grinding and mechanical manipulation was obvious in the okara sample prepared with 3 and 5% RRS, where GMO content was reduced to (2...
December 2017: Food Technology and Biotechnology
https://www.readbyqxmd.com/read/29532426/lyotropic-liquid-crystalline-nanoparticles-of-amphotericin-b-implication-of-phytantriol-and-glyceryl-monooleate-on-bioavailability-enhancement
#17
Sanyog Jain, Pooja Yadav, Rajan Swami, Nitin Kumar Swarnakar, Varun Kushwah, Sameer S Katiyar
Implication of different dietary specific lipids such as phytantriol (PT) and glyceryl monooleate (GMO) on enhancing the oral bioavailability of amphotericin B (AmB) was examined. Liquid crystalline nanoparticles (LCNPs) were prepared using hydrotrope method, followed by in vitro characterization, Caco-2 cell monolayer uptake, and in vivo pharmacokinetic and toxicity evaluation. Optimized AmB-LCNPs displayed small particle size (< 210 nm) with a narrow distribution (~ 0.2), sustained drug release and high gastrointestinal stability, and reduced hemolytic toxicity...
May 2018: AAPS PharmSciTech
https://www.readbyqxmd.com/read/29509719/gene-editing-of-microalgae-scientific-progress-and-regulatory-challenges-in-europe
#18
REVIEW
Andrew Spicer, Attila Molnar
It is abundantly clear that the development of gene editing technologies, represents a potentially powerful force for good with regard to human and animal health and addressing the challenges we continue to face in a growing global population. This now includes the development of approaches to modify microalgal strains for potential improvements in productivity, robustness, harvestability, processability, nutritional composition, and application. The rapid emergence and ongoing developments in this area demand a timely review and revision of the current definitions and regulations around genetically modified organisms (GMOs), particularly within Europe...
March 6, 2018: Biology
https://www.readbyqxmd.com/read/29488171/evaluation-and-optimization-of-ph-responsive-niosomes-as-a-carrier-for-efficient-treatment-of-breast-cancer
#19
Heba F Salem, Rasha M Kharshoum, Fatma I Abo El-Ela, Amr Gamal F, Khaled R A Abdellatif
Tamoxifen citrate (TXC) is commonly indicated to prevent cell multiplication and development of breast cancer. However, it is usually associated with limited activity and development of toxicity and resistance. This study aimed to describe an in situ pH-responsive niosomes as a carrier for localized and sustained delivery of TXC. The thin film hydration method was utilized to produce TXC niosomes using sorbitan monostearate and cholesterol of 1:1 Molar ratio. The produced formula displayed nano-spherical shape with entrapment efficiency (EE) of 88...
June 2018: Drug Delivery and Translational Research
https://www.readbyqxmd.com/read/29406548/a-liquid-crystalline-precursor-incorporating-chlorhexidine-acetate-and-silver-nanoparticles-for-root-canal-disinfection
#20
Tengyi Zheng, Xintian Huang, Jintian Chen, Disang Feng, Liling Mei, Ying Huang, Guilan Quan, Chune Zhu, Vikramjeet Singh, Hao Ran, Xin Pan, Chuan-Yu Wu, Chuanbin Wu
Lyotropic liquid crystals (LLC) have received increasing attention as a drug delivery system. In this study, a novel intra-canal disinfectant based on the glycerol monooleate (GMO) LLC precursor incorporation with chlorhexidine (CHX) and silver nanoparticles (Ag-NPs) was designed and evaluated. The LLC precursor with excellent fluidity was able to penetrate deeply into the complex tiny collateral branch root canals. The transformation of cubic LLC in root canals upon coming into contact with water provided long-lasting disinfection against multidrug-resistant bacteria to avoid the endodontic reinfection and follow-up visits...
February 27, 2018: Biomaterials Science
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