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https://www.readbyqxmd.com/read/28071020/the-effect-of-microwave-roasting-on-the-antioxidant-properties-of-the-bangladeshi-groundnut-cultivar
#1
Abbas Ali, Anowarul Islam, Tarun K Pal
BACKGROUND: Groundnut seeds are an important source of bioactive phenolic compounds with noteworthy antioxidant capacity, which may be enhanced by the microwave roasting process. The aim of this work is   to study the changes in antioxidant activity in groundnut seeds during microwave roasting, as a function of roasting time and extract concentration, in order to maximise the phenolic content and antioxidant activity of roasted seeds. METHODS: The study was conducted to evaluate total phenolic content (TPC), total flavonoid content (TFC), and antioxidative activity of methanolic (GME), ethanolic (GEE), and chloroform (GCE) extracts and methanolic extract of oil (GMO) from groundnut seeds exposed to microwaves...
October 2016: Acta Scientiarum Polonorum. Technologia Alimentaria
https://www.readbyqxmd.com/read/28064126/on-site-detection-of-stacked-genetically-modified-soybean-based-on-event-specific-tm-lamp-and-a-dnazyme-lateral-flow-biosensor
#2
Nan Cheng, Ying Shang, Yuancong Xu, Li Zhang, Yunbo Luo, Kunlun Huang, Wentao Xu
Stacked genetically modified organisms (GMO) are becoming popular for their enhanced production efficiency and improved functional properties, and on-site detection of stacked GMO is an urgent challenge to be solved. In this study, we developed a cascade system combining event-specific tag-labeled multiplex LAMP with a DNAzyme-lateral flow biosensor for reliable detection of stacked events (DP305423× GTS 40-3-2). Three primer sets, both event-specific and soybean species-specific, were newly designed for the tag-labeled multiplex LAMP system...
December 30, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28055985/facile-synthesis-and-enhanced-photocatalytic-activity-of-single-crystalline-nanohybrids-for-removal-of-organic-pollutants
#3
Erum Pervaiz, Honghong Liu, Minghui Yang
Present study focused the synthesis of α-MoO3/rGO. One dimensional nano hybrids under mild conditions and a low temperature wet chemical route produced a single, highly pure crystalline orthorhombic α-MoO3 on graphene oxide (GO) sheets. Four nano hybrids as GMO-0, GMO-1, GMO-2, and GMO-3 has been synthesized with a different mass, charging of GO as 0 mg, 40 mg, 60 mg and 100 mg respectively. Photocatalytic performance for organic pollutant reduction has been analyzed. Presence of different amounts of GO in the prepared metal oxide hybrids alters performance of the material as elaborated by Brunauer-Emmett-Teller (BET) surface area, UV-Visible diffused reflectance spectra and resulted in reduction of organic dyes depicted by photocatalytic experiments...
January 5, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28028794/effect-of-liquid-crystalline-systems-containing-antimicrobial-compounds-on-infectious-skin-bacteria
#4
Carla Souza, Evandro Watanabe, Carolina Patrícia Aires, Marilisa Guimarães Lara
This study aimed (i) to prepare liquid crystalline systems (LCS) of glyceryl monooleate (GMO) and water containing antibacterial compounds and (ii) to evaluate their potential as drug delivery systems for topical treatment of bacterial infections. Therefore, LCS containing CPC (cetylpyridinium chloride) (LCS/CPC) and PHMB (poly(hexamethylene biguanide) hydrochloride) (LCS/PHMB) were prepared and the liquid crystalline phases were identified by polarizing light microscopy 24 h and 7 days after preparation...
December 27, 2016: AAPS PharmSciTech
https://www.readbyqxmd.com/read/28025452/development-and-evaluation-of-event-specific-quantitative-pcr-method-for-genetically-modified-soybean-mon87701
#5
Keita Tsukahara, Reona Takabatake, Tomoko Masubuchi, Satoshi Futo, Yasutaka Minegishi, Akio Noguchi, Kazunari Kondo, Tomoko Nishimaki-Mogami, Takeyo Kurashima, Junichi Mano, Kazumi Kitta
A real-time PCR-based analytical method was developed for the event-specific quantification of a genetically modified (GM) soybean event, MON87701. First, a standard plasmid for MON87701 quantification was constructed. The conversion factor (Cf) required to calculate the amount of genetically modified organism (GMO) was experimentally determined for a real-time PCR instrument. The determined Cf for the real-time PCR instrument was 1.24. For the evaluation of the developed method, a blind test was carried out in an inter-laboratory trial...
2016: Shokuhin Eiseigaku Zasshi. Journal of the Food Hygienic Society of Japan
https://www.readbyqxmd.com/read/28001470/the-eu-legislation-on-gmos-between-nonsense-and-protectionism-an-ongoing-schumpeterian-chain-of-public-choices
#6
Giovanni Tagliabue
The EU regulation of agricultural biotechnology is botched and convoluted: the pseudo-concept of "Genetically Modified Organisms" has no coherent semantic or scientific content. The reasons of the paradox by which the cultivation of "GMOs" is substantially banned in Europe, while enormous quantities of recombinant-DNA cereals and legumes are imported to be used as feedstuff, are explained. The Directive 2015/412, giving Member states the choice to refuse the cultivation of genetically engineered crops at a national or local level, paves the way for a mosaic-like, Harlequinesque form of protectionism: nothing resembling a well-regulated free market...
December 21, 2016: GM Crops & Food
https://www.readbyqxmd.com/read/27909941/degradation-and-half-life-of-dna-present-in-biomass-from-a-genetically-modified-organism-during-land-application
#7
Mathew C Halter, James A Zahn
White biotechnology has made a positive impact on the chemical industry by providing safer, more efficient chemical manufacturing processes that have reduced the use of toxic chemicals, harsh reaction conditions, and expensive metal catalysts, which has improved alignment with the principles of Green Chemistry. The genetically-modified (GM) biocatalysts that are utilized in these processes are typically separated from high-value products and then recycled, or eliminated. Elimination routes include disposal in sanitary landfills, incineration, use as a fuel, animal feed, or reuse as an agricultural soil amendment or other value-added products...
December 1, 2016: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27768244/detection-limits-of-quantitative-and-digital-pcr-assays-and-their-influence-in-presence-absence-surveys-of-environmental-dna
#8
Margaret E Hunter, Robert M Dorazio, John S S Butterfield, Gaia Meigs-Friend, Leo G Nico, Jason A Ferrante
A set of universal guidelines is needed to determine the limit of detection (LOD) in PCR-based analyses of low-concentration DNA. In particular, environmental DNA (eDNA) studies require sensitive and reliable methods to detect rare and cryptic species through shed genetic material in environmental samples. Current strategies for assessing detection limits of eDNA are either too stringent or subjective, possibly resulting in biased estimates of species' presence. Here, a conservative LOD analysis grounded in analytical chemistry is proposed to correct for overestimated DNA concentrations predominantly caused by the concentration plateau, a nonlinear relationship between expected and measured DNA concentrations...
October 21, 2016: Molecular Ecology Resources
https://www.readbyqxmd.com/read/27744147/optimization-of-identity-operation-in-nmr-spectroscopy-via-genetic-algorithm-application-to-the-tedor-experiment
#9
V S Manu, Gianluigi Veglia
Identity operation in the form of π pulses is widely used in NMR spectroscopy. For an isolated single spin system, a sequence of even number of π pulses performs an identity operation, leaving the spin state essentially unaltered. For multi-spin systems, trains of π pulses with appropriate phases and time delays modulate the spin Hamiltonian to perform operations such as decoupling and recoupling. However, experimental imperfections often jeopardize the outcome, leading to severe losses in sensitivity. Here, we demonstrate that a newly designed Genetic Algorithm (GA) is able to optimize a train of π pulses, resulting in a robust identity operation...
September 27, 2016: Journal of Magnetic Resonance
https://www.readbyqxmd.com/read/27739510/multiplex-quantification-of-four-dna-targets-in-one-reaction-with-bio-rad-droplet-digital-pcr-system-for-gmo-detection
#10
David Dobnik, Dejan Štebih, Andrej Blejec, Dany Morisset, Jana Žel
The advantages of the digital PCR technology are already well documented until now. One way to achieve better cost efficiency of the technique is to use it in a multiplexing strategy. Droplet digital PCR platforms, which include two fluorescence filters, support at least duplex reactions and with some developments and optimization higher multiplexing is possible. The present study not only shows a development of multiplex assays in droplet digital PCR, but also presents a first thorough evaluation of several parameters in such multiplex digital PCR...
October 14, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27727209/bypassing-gmo-regulations-with-crispr-gene-editing
#11
Jungeun Kim, Jin-Soo Kim
No abstract text is available yet for this article.
October 11, 2016: Nature Biotechnology
https://www.readbyqxmd.com/read/27695818/nobel-laureates-pick-food-fight-with-gmo-foes
#12
Jeff Lyon
No abstract text is available yet for this article.
November 1, 2016: JAMA: the Journal of the American Medical Association
https://www.readbyqxmd.com/read/27642670/delivery-of-dual-drug-loaded-lipid-based-nanoparticles-across-the-blood-brain-barrier-impart-enhanced-neuroprotection-in-a-rotenone-induced-mouse-model-of-parkinson-s-disease
#13
Paromita Kundu, Manasi Das, Kalpalata Tripathy, Sanjeeb K Sahoo
Parkinson's disease (PD) is the most widespread form of dementia where there is an age related degeneration of dopaminergic neurons in the substantia nigra region of the brain. Accumulation of α-synuclein (αS) protein aggregate, mitochondrial dysfunction, oxidative stress, and neuronal cell death are the pathological hallmarks of PD. In this context, amalgamation of curcumin and piperine having profound cognitive properties, and antioxidant activity seems beneficial. However, the blood-brain barrier (BBB) is the major impediment for delivery of neurotherapeutics to the brain...
December 21, 2016: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/27632191/comparison-of-droplet-digital-pcr-with-quantitative-real-time-pcr-for-determination-of-zygosity-in-transgenic-maize
#14
Xiaoli Xu, Cheng Peng, Xiaofu Wang, Xiaoyun Chen, Qiang Wang, Junfeng Xu
This study evaluated the applicability of droplet digital PCR (ddPCR) as a tool for maize zygosity determination using quantitative real-time PCR (qPCR) as a reference technology. Quantitative real-time PCR is commonly used to determine transgene copy number or GMO zygosity characterization. However, its effectiveness is based on identical reaction efficiencies for the transgene and the endogenous reference gene. Additionally, a calibrator sample should be utilized for accuracy. Droplet digital PCR is a DNA molecule counting technique that directly counts the absolute number of target and reference DNA molecules in a sample, independent of assay efficiency or external calibrators...
September 8, 2016: Transgenic Research
https://www.readbyqxmd.com/read/27627879/identification-of-target-genes-to-control-acetate-yield-during-aerobic-fermentation-with-saccharomyces-cerevisiae
#15
José Antonio Curiel, Zoel Salvadó, Jordi Tronchoni, Pilar Morales, Alda Joao Rodrigues, Manuel Quirós, Ramón Gonzalez
BACKGROUND: Aerobic fermentation of grape must, leading to respiro-fermentative metabolism of sugars, has been proposed as way of reducing alcohol content in wines. Two factors limit the usefulness of Saccharomyces cerevisiae for this application, the Crabtree effect, and excess volatile acidity under aerobic conditions. This work aimed to explore the impact on ethanol acetate production of different S. cerevisiae strains deleted for genes previously related with the Crabtree phenotype...
September 15, 2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/27626052/data-on-screening-and-identification-of-genetically-modified-papaya-in-food-supplements
#16
Theo W Prins, Ingrid M J Scholtens, Arno W Bak, Jeroen P van Dijk, Marleen M Voorhuijzen, Emile J Laurensse, Esther J Kok
This article contains data related to the research article entitled "A case study to determine the geographical origin of unknown GM papaya in routine food sample analysis, followed by identification of papaya events 16-0-1 and 18-2-4" (Prins et al., 2016) [1]. Quantitative real-time PCR (qPCR) with targets that are putatively present in genetically modified (GM) papaya was used as a first screening to narrow down the vast array of candidates. The combination of elements P-nos and nptII was further confirmed by amplification and subsequent sequencing of the P-nos/nptII construct...
December 2016: Data in Brief
https://www.readbyqxmd.com/read/27607730/role-of-ytterbium-erbium-co-doped-gadolinium-molybdate-gd-sub-2-sub-moo-sub-4-sub-sub-3-sub-yb-er-nanophosphors-in-solar-cells
#17
Xiao Jin, Haiyang Li, Dongyu Li, Qin Zhang, Feng Li, Weifu Sun, Zihan Chen, Qinghua Li
Insufficient harvest of solar light energy is one of the obstacles for current photovoltaic devices to achieve high performance. Especially, conventional organic/inorganic hybrid solar cells (HSCs) based on PTB7 as p-type semiconductor can only utilize 400-800 nm solar spectrum. One effective strategy to overcome this obstacle is the introduction of up-conversion nanophosphors (NPs), in the virtue of utilizing the near infrared region (NIR) of solar radiation. Up-conversion can convert low-energy photons to high-energy ones through multi-photon processes, by which the solar spectrum is tailored to well match the absorptive domain of the absorber...
September 5, 2016: Optics Express
https://www.readbyqxmd.com/read/27606259/development-and-characterization-of-novel-hydrogel-containing-antimicrobial-drug-for-treatment-of-burns
#18
Vaishali Thakkar, Vaishali Korat, Lalji Baldaniya, Mukesh Gohel, Tejal Gandhi, Nirav Patel
INTRODUCTION: The aim of burn management and therapy is fast healing and epithelisation to prevent infection. The present study is concerned with the development and characterization of a novel nanaoparticulate system; cubosomes, loaded with silver sulfadiazine (SSD) and Aloe vera for topical treatment of infected burns. METHODS: Cubosome dispersions were formulated by an emulsification technique using different concentrations of a lipid phase Glyceryl Monooleate (GMO) and Poloxamer 407...
July 2016: International Journal of Pharmaceutical Investigation
https://www.readbyqxmd.com/read/27576669/exploring-the-effect-of-hydrophilic-and-hydrophobic-structure-of-grafted-polymeric-micelles-on-drug-loading
#19
Chenjun Shi, Yujiao Sun, Haiyang Wu, Chengyun Zhu, Guoguang Wei, Jinfeng Li, Tenglan Chan, Defang Ouyang, Shirui Mao
The objective of this paper is to explore the effect of hydrophilic and hydrophobic structure of grafted polymeric micelles on drug loading, and elucidate whether drug-polymer compatibility, as predicted by Hansen solubility parameters (HSPs), can be used as a tool for drug-polymer pairs screening and guide the design of grafted polymeric micelles. HSPs of 27 drugs and three grafted copolymers were calculated according to group contribution method. The drug-polymer compatibilities were evaluated using the approaches of Flory-Huggins interaction parameters (χFH) and polarity difference (△Xp)...
October 15, 2016: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/27573438/car1-deletion-by-crispr-cas9-reduces-formation-of-ethyl-carbamate-from-ethanol-fermentation-by-saccharomyces-cerevisiae
#20
Young-Wook Chin, Woo-Kyung Kang, Hae Won Jang, Timothy L Turner, Hyo Jin Kim
Enormous advances in genome editing technology have been achieved in recent decades. Among newly born genome editing technologies, CRISPR/Cas9 is considered revolutionary because it is easy to use and highly precise for editing genes in target organisms. CRISPR/Cas9 technology has also been applied for removing unfavorable target genes. In this study, we used CRISPR/Cas9 technology to reduce ethyl carbamate (EC), a potential carcinogen, which was formed during the ethanol fermentation process by yeast. Because the yeast CAR1 gene encoding arginase is the key gene to form ethyl carbamate, we inactivated the yeast CAR1 gene by the complete deletion of the gene or the introduction of a nonsense mutation in the CAR1 locus using CRISPR/Cas9 technology...
November 2016: Journal of Industrial Microbiology & Biotechnology
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