M Richardson, A M C Pimenta, M P Bemquerer, M M Santoro, P S L Beirao, M E Lima, S G Figueiredo, C Bloch, E A R Vasconcelos, F A P Campos, P C Gomes, M N Cordeiro
The proteomes of the venoms of the Brazilian wandering "armed" spiders Phoneutria nigriventer, Phoneutria reidyi, and Phoneutria keyserlingi, were compared using two-dimensional gel electrophoresis. The venom components were also fractionated using a combination of preparative reverse phase HPLC on Vydac C4, analytical RP-HPLC on Vydac C8 and C18 and cation exchange FPLC on Resource S at pH 6.1 and 4.7, or anion exchange HPLC on Synchropak AX-300 at pH 8.6. The amino acid sequences of the native and S-pyridyl-ethylated proteins and peptides derived from them by enzymatic digestion and chemical cleavages were determined using a Shimadzu PPSQ-21(A) automated protein sequencer, and by MS/MS collision induced dissociations...
March 2006: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP