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https://www.readbyqxmd.com/read/28330307/effect-of-particle-size-moisture-content-and-supplements-on-selective-pretreatment-of-cotton-stalks-by-daedalea-flavida-and-enzymatic-saccharification
#1
Harmanpreet Meehnian, Asim K Jana, Mithu Maiti Jana
A large amount of cotton stalk waste biomass with high cellulose content are incinerated by the farmers causing air pollution. The high cellulose content of cotton stalks can be converted to fermentable sugars by fungal delignification pretreatment of lignocellulosic biomass and enzymatic saccharification. The effect of particle size, moisture content, and media supplements was studied for delignification of cotton stalks by Daedalea flavida MTCC 145 (DF-2) in solid-state fermentation. The highest lignolytic enzyme activities, optimal lignin degradation 29...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330256/immobilized-lipase-catalyzing-glucose-stearate-synthesis-and-their-surfactant-properties-analysis
#2
A Maria Sebatini, Manisha Jain, P Radha, S Kiruthika, Krishnamurthi Tamilarasan
Sugar fatty acid esters are practical importance and have a variety of applications that include surfactants and as an emulsifying agent. In this study, we report glucose stearate synthesis using lipase-Fe3O4 nanoparticles catalyst. The influence of various reaction factors, such as silica gel concentration, molar ratio of sugar/acid, reaction temperature and speed of agitation on esterification by immobilized enzyme was analyzed. The glucose stearate esterification degree of 87.2 % was obtained under the optimized condition: 1:2 molar ratio of glucose/stearic acid, 2 % (w/v) of silica gel at 120 rpm and 40 °C...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330240/a-novel-cyclodextrin-glucanotransferase-from-an-alkaliphile-microbacterium-terrae-knr-9-purification-and-properties
#3
Kiransinh N Rajput, Kamlesh C Patel, Ujjval B Trivedi
Cyclodextrin glucanotransferase (CGTase, EC. 2.1.1.19) produced using new alkaliphile Microbacterium terrae KNR 9 has been purified to homogeneity in a single step by the starch adsorption method. The specific activity of the purified CGTase was 45 U/mg compared to crude 0.9 U/mg. This resulted in a 50-fold purification of the enzyme with 33 % yield. The molecular weight of the purified enzyme was found to be 27.72 kDa as determined by SDS-PAGE. Non-denaturing gel electrophoresis and activity staining confirmed the presence of CGTase in crude and the ammonium sulfate precipitate fraction...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330234/optimization-of-cellulase-production-by-penicillium-sp
#4
H N Prasanna, G Ramanjaneyulu, B Rajasekhar Reddy
The production of cellulolytic enzymes (β-exoglucanase, β-endoglucanase and β-glucosidase) by Penicillium sp. on three different media in liquid shake culture conditions was compared. The organism exhibited relatively highest activity of endoglucanase among three enzymes measured at 7-day interval during the course of its growth on Czapek-Dox medium supplemented with 0.5 % (w/v) cellulose. Cellulose at 0.5 %, lactose at 0.5 %, sawdust at 0.5 %, yeast extract at 0.2 % as a nitrogen source, pH 5.0 and 30 °C temperature were found to be optimal for growth and cellulase production by Penicillium sp...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330216/potential-application-of-waste-from-castor-bean-ricinus-communis-l-for-production-for-xylanase-of-interest-in-the-industry
#5
Polyanna Nunes Herculano, Keila Aparecida Moreira, Raquel Pedrosa Bezerra, Tatiana Souza Porto, Cristina Maria de Souza-Motta, Ana Lúcia Figueiredo Porto
Xylanases activity (XY) from Aspergillus japonicus URM5620 produced by Solid-State Fermentation (SSF) of castor press cake (Ricinus communis) on different conditions of production and extraction by PEG/citrate aqueous two-phase system (ATPS) were investigated. XY production was influenced by substrate amount (5-10 g), initial moisture (15-35 %), pH (4.0-6.0) and temperature (25-35 °C), obtaining the maximum activity of 29,085 ± 1808 U g ds(-1) using 5.0 g of substrate with initial moisture of 15 % at 25 °C and pH 6...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330214/production-kinetics-of-polyhydroxyalkanoates-by-using-pseudomonas-aeruginosa-gamma-ray-mutant-strain-ebn-8-cultured-on-soybean-oil
#6
Sharjeel Abid, Zulfiqar Ali Raza, Tanveer Hussain
The purpose of present study was to optimize polyhydroxyalkanotes (PHAs) production in a gamma ray mutant strain of Pseudomonas aeruginosa grown on soybean oil in minimal salts media under shake flask conditions. The production kinetics was studied by sampling on daily basis for 6 days to investigate the best conditions for PHAs production like biomass estimation, carbon source utilization and PHAs yield. The PHA accumulation was observed up to 50.27 % (w/w) of cell dry mass. The Pseudomonas species synthesized medium chain length PHA copolyester as per identified by LCMS and confirmed by FTIR spectroscopy...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330211/cellulase-production-using-natural-medium-and-its-application-on-enzymatic-hydrolysis-of-thermo-chemically-pretreated-biomass
#7
Shivani Sharma, Vinay Sharma, Arindam Kuila
Lignocellulosic bioethanol is an important renewable fuel for transportation purpose. Commercial production of lignocellulosic bioethanol mainly depends on cost of cellulase production, efficient pretreatment and enzymatic hydrolysis process. In the present study cellulase production from Aspergillus niger under submerged fermentation (SmF) was optimized using coconut water as natural medium. Maximum cellulase production (0.53 IU/mL) was achieved within 3 days of incubation using 8 % (w/v) waste paper and 0...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330203/evaluation-of-several-ionic-liquids-for-in-situ-hydrolysis-of-empty-fruit-bunches-by-locally-produced-cellulase
#8
Amal Ahmed Elgharbawy, Md Zahangir Alam, Nassereldeen Ahmad Kabbashi, Muhammad Moniruzzaman, Parveen Jamal
Lignocellulosic biomasses, exhibit resistance to enzymatic hydrolysis due to the presence of lignin and hemicellulose. Ionic liquids proved their applicability in lignin degradation, however, ionic liquid removal has to be performed to proceed to hydrolysis. Therefore, this study reports an in situ hydrolysis of empty fruit bunches (EFB) that combined an ionic liquid (IL) pretreatment and enzymatic hydrolysis. For enzyme production, palm kernel cake (PKC) was used as the primary media for microbial cellulase (PKC-Cel) from Trichoderma reesei (RUTC30)...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330188/purification-and-characterization-of-a-surfactant-compatible-lipase-from-aspergillus-tamarii-jgif06-exhibiting-energy-efficient-removal-of-oil-stains-from-polycotton-fabric
#9
Arijit Das, Srividya Shivakumar, Sourav Bhattacharya, Sujina Shakya, S S Swathi
An extracellular lipase with 23,666.66 U/ml/min activity was produced by Aspergillus tamarii JGIF06 under submerged fermentation in mineral salt medium containing coconut oil (2.5 % v/v), tryptone (2 % w/v) and ammonium chloride (2 % w/v), with initial pH of 5 ± 0.2, incubated at 25 °C for 7 days on a rotary shaker at 120 rpm. A 7.9-fold increase in lipase-specific activity was recorded after purification by DEAE Sepharose ion exchange and Sephadex G200 column chromatography. The apparent molecular mass of this enzyme was revealed as 50 kDa by sodium dodecyl sulphate polyacrylamide gel electrophoresis...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330169/investigating-the-effect-of-drought-stress-on-expression-of-wrky1-and-erebp1-genes-and-antioxidant-enzyme-activities-in-lemon-balm-melissa-officinalis-l
#10
Mehdi Rahimi, Mojtaba Kordrostami, Mahmood Maleki, Mohsen ModaresKia
Drought stress is a severe environmental constraint to plant productivity. In this study, gene expression of WRKY1 and EREBP1 genes and activity of two enzymes were investigated in two genotypes, G9 (as resistance) and G12 (as susceptible) of 10 days-old lemon balm seedlings. Experiments were done according to factorial design on the base of completely randomized design with three replications for expression analysis and enzyme assays. Seedlings were cultured in MS medium suspensions, including 0, 3, 6, 12 and 15 % w/v PEG 6000...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330139/production-of-bioethanol-as-useful-biofuel-through-the-bioconversion-of-water-hyacinth-eichhornia-crassipes
#11
Arpan Das, Priyanka Ghosh, Tanmay Paul, Uma Ghosh, Bikas Ranjan Pati, Keshab Chandra Mondal
Water hyacinth (Eichhornia crassipes) represents a promising candidate for fuel ethanol production in tropical countries because of their high availability and high biomass yield. Bioconversion of such biomass to bioethanol could be wisely managed through proper technological approach. In this work, pretreatment of water hyacinth (10 %, w/v) with dilute sulfuric acid (2 %, v/v) at high temperature and pressure was integrated in the simulation and economic assessment of the process for further enzymatic saccharification was studied...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330132/an-efficient-and-cost-effective-method-for-dna-extraction-from-athalassohaline-soil-using-a-newly-formulated-cell-extraction-buffer
#12
Avinash Narayan, Kunal Jain, Amita R Shah, Datta Madamwar
The present study describes the rapid and efficient indirect lysis method for environmental DNA extraction from athalassohaline soil by newly formulated cell extraction buffer. The available methods are mostly based on direct lysis which leads to DNA shearing and co-extraction of extra cellular DNA that influences the community and functional analysis. Moreover, during extraction of DNA by direct lysis from athalassohaline soil, it was observed that, upon addition of poly ethylene glycol (PEG), isopropanol or absolute ethanol for precipitation of DNA, salt precipitates out and affecting DNA yield significantly...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330106/enhanced-production-of-pectinase-by-aspergillus-terreus-ncft-4269-10-using-banana-peels-as-substrate
#13
Bijay Kumar Sethi, Prativa Kumari Nanda, Santilata Sahoo
Aspergillus terreus NCFT4269.10 was implemented in solid-state (SSF) and liquid static surface fermentation (LSSF) for biosynthesis of pectinase. Amongst various substrates, like, mustard oil cake, neem oil cake, groundnut oil cake, black gram peels, green gram peels, chickling vetch peels/grass pea peels wheat bran, pearl millet residues, finger millet waste, broken rice, banana peels (BP), apple pomace (AP) and orange peels, banana peel (Musa paradisiaca L.; Family: Musaceae) was most suitable for pectinase biosynthesis (LSSF: 400 ± 21...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330083/enzymatic-production-of-glucosamine-and-chitooligosaccharides-using-newly-isolated-exo-%C3%AE-d-glucosaminidase-having-transglycosylation-activity
#14
Sujata Sinha, Subhash Chand, Pushplata Tripathi
Exochitosanase secreting fungus (A. fumigatus IIT-004) was isolated from fish waste using 1 % (w/v) chitosan as sole carbon source after multistage screening. Chitosan-dependent exochitosanase enzyme production (6 IU ml(-1)) in log phase of growth (chitosan utilization rate 0.11 g g(-1) cell h(-1)) was observed for Aspergillus fumigatus in chitosan minimal salt medium and there was no enzyme production in glucose medium. Enzyme production was found to be extracellular and subjected to purification by a number of steps like acetone fractionation as well as column chromatography...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330053/importance-des-variations-spectrales-durant-36%C3%A2-mois-de-suivi-longitudinal-irm-et-srm-de-80%C3%A2-patients-atteints-de-glioblastomes-trait%C3%A3-s
#15
J-M Constans, A Heintz, O Seloi, N Deleval, M Beauvois, R Hanafi, F Dallery, W Dou, S Ruan, J Prades, D Le Gars, O Baledent, H Deramond, A Houessinon, A Fichten, M Lefranc, A Coutte, P Toussaint, C Desenclos, B Chauffert, M Boone
No abstract text is available yet for this article.
March 2017: Journal of Neuroradiology. Journal de Neuroradiologie
https://www.readbyqxmd.com/read/28330034/enc%C3%A3-phalite-virale-dans-toutes-ses-formes%C3%A2-comment-s%C3%A2-en-sortir%C3%A2
#16
A Khalfalli, B Hmida, H Harres, M Maatouk, S Jamel, W Mnari, A Zrig, R Salem, M Golli
No abstract text is available yet for this article.
March 2017: Journal of Neuroradiology. Journal de Neuroradiologie
https://www.readbyqxmd.com/read/28330028/int%C3%A3-r%C3%A3%C2%AAt-des-s%C3%A3-quences-avanc%C3%A3-es-en-irm-dans-l%C3%A2-%C3%A3-valuation-%C3%A3-la-r%C3%A3-ponse-au-bevacizumab-suivi-multimodal-de-20%C3%A2-glioblastomes
#17
O Seloi, A Heintz, R Hanafi, R Laborde, W Dou, S Ruan, J Prades, D Le Gars, H Deramond, M Lefranc, A Coutte, P Toussaint, C Desenclos, B Chauffert, M Boone, J M Constans
No abstract text is available yet for this article.
March 2017: Journal of Neuroradiology. Journal de Neuroradiologie
https://www.readbyqxmd.com/read/28329978/tumeurs-c%C3%A3-r%C3%A3-brales-sustentorielles-de-l%C3%A2-adulte%C3%A2-comment-raisonner-avec-l%C3%A2-irm-multimodale%C3%A2
#18
A Khalfalli, B Hmida, R Hamdi, M Maatouk, S Jamel, W Mnari, A Zrig, R Salem, M Golli
No abstract text is available yet for this article.
March 2017: Journal of Neuroradiology. Journal de Neuroradiologie
https://www.readbyqxmd.com/read/28329948/-evaluation-of-estimation-of-prevalence-ratio-using-bayesian-log-binomial-regression-model
#19
W L Gao, H Lin, X N Liu, X W Ren, J S Li, X P Shen, S L Zhu
To evaluate the estimation of prevalence ratio (PR) by using bayesian log-binomial regression model and its application, we estimated the PR of medical care-seeking prevalence to caregivers' recognition of risk signs of diarrhea in their infants by using bayesian log-binomial regression model in Openbugs software. The results showed that caregivers' recognition of infant' s risk signs of diarrhea was associated significantly with a 13% increase of medical care-seeking. Meanwhile, we compared the differences in PR's point estimation and its interval estimation of medical care-seeking prevalence to caregivers' recognition of risk signs of diarrhea and convergence of three models (model 1: not adjusting for the covariates; model 2: adjusting for duration of caregivers' education, model 3: adjusting for distance between village and township and child month-age based on model 2) between bayesian log-binomial regression model and conventional log-binomial regression model...
March 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://www.readbyqxmd.com/read/28329946/-a-cohort-study-on-association-between-the-first-trimester-phthalates-exposure-and-fasting-blood-glucose-level-in-the-third-trimester
#20
Y W Zhang, H Gao, K Huang, Y Y Xu, J Sheng, F B Tao
Objective: To examine the association between the phthalate exposure in the first trimester and fasting blood glucose level or gestational diabetes mellitus (GDM) in the third trimester in pregnant women. Methods: A total of 3 474 pregnant women, receiving their prenatal examination in Ma' anshan Maternal and Child Health-Care Hospital of Anhui province, were selected from May 2013 to September 2014. Questionnaires were used to collect the information about their socio-demographic characteristics, clinical characteristics and GDM diagnostic results in the first, second and third trimesters...
March 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
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