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https://www.readbyqxmd.com/read/29212377/gene-therapy-for-bone-repair-using-human-cells-superior-osteogenic-potential-of-bmp-2-transduced-mesenchymal-stem-cells-derived-from-adipose-tissue-compared-to-bone-marrow
#1
Sofia Bougioukli, Osamu Sugiyama, William Pannell, Brandon Ortega, Matthew Tan, Amy Tang, Robert Yoho, Daniel A Oakes, Jay R Lieberman
Ex vivo regional gene therapy strategies using animal mesenchymal stem cells genetically modified to overexpress osteoinductive growth factors has been successfully used in a variety of animal models to induce both heterotopic and orthotopic bone formation. However, in order to adapt regional gene therapy for clinical applications it is essential to assess the osteogenic capacity of transduced human cells and choose the cell type that demonstrates the best clinical potential. Bone marrow stem cells (BMSC) and adipose-derived stem cells (ASC) were selected in our study for in vitro evaluation, before and after transduction with a lentiviral two-step transcriptional amplification system (TSTA) overexpressing BMP-2 (LV-TSTA-BMP-2) or GFP (LV-TSTA-GFP); cell growth, transduction efficiency, BMP-2 production and osteogenic capacity were assessed...
December 6, 2017: Human Gene Therapy
https://www.readbyqxmd.com/read/29186653/mesenchymal-stem-cell-differentiation-into-adipocytes-is-equally-induced-by-insulin-and-proinsulin-in-vitro
#2
Andreas Pfützner, Dorothee Schipper, Andreas Pansky, Claudia Kleinfeld, Barbara Roitzheim, Edda Tobiasch
Background and Objectives: In advanced β-cell dysfunction, proinsulin is increasingly replacing insulin as major component of the secretion product. It has been speculated that proinsulin has at least the same adipogenic potency than insulin, leading to an increased tendency of lipid tissue formation in patients with late stage β-cell dysfunction. Methods and Results: Mesenchymal stem cells obtained from liposuction material were grown in differentiation media containing insulin (0...
November 30, 2017: International Journal of Stem Cells
https://www.readbyqxmd.com/read/29184746/strategies-for-reducing-fatal-complications-in-liposuction
#3
Lázaro Cárdenas-Camarena, Lozano-Peña Andrés Gerardo, Héctor Durán, Jorge Enrique Bayter-Marin
Background: Liposuction has become one of the most common cosmetic surgical procedures, and severe complications secondary to this procedure have also increased significantly. That is why we carry out a research work to know the most frequent severe complications reported in the scientific literature to indicate criteria for prevention. Methods: English-language scientific publications about liposuction and its complications were analyzed using the PubMed.gov, from the beginning of PubMed's history through June 10, 2017...
October 2017: Plastic and Reconstructive Surgery. Global Open
https://www.readbyqxmd.com/read/29175136/the-liquid-facelift-first-hands-on-experience-with-facial-water-jet-assisted-liposuction-as-an-additive-technique-for-rhytidectomy-a-case-series-of-25-patients
#4
Kai O Kaye, Sonja Kästner, Felix J Paprottka, Phillipp Gonser
OBJECTIVE: Recently, water jet-assisted liposuction (WAL) was successfully applied by several other authors to remove fat and induce skin contraction in nonfacial body areas. Extending the range of indications for this new method, the authors of this article report on their first experience with its use in facial contouring, fat harvesting, and hydrodissection of the facial skin flap in rhytidectomy in a case series of 25 patients. METHODS: Twenty-five patients (median age: 56 years) had facelift surgery under sedation, and the WAL technique was used for facial contouring, fat harvesting for facial lipotransfer, and hydrodissection of the facial skin flap...
November 2, 2017: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
https://www.readbyqxmd.com/read/29160142/structural-fat-grafting-to-improve-outcomes-of-vocal-folds-fat-augmentation-long-term-results
#5
Giovanna Cantarella, Riccardo F Mazzola, Michele Gaffuri, Elisabetta Iofrida, Pietro Biondetti, Laura V Forzenigo, Lorenzo Pignataro, Sara Torretta
Objective Evaluating the long-term outcomes of vocal fold structural fat grafting. Study Design Case series with chart review. Setting University hospital. Subjects and Methods Seventy-nine dysphonic patients (16-82 years; 55 with unilateral laryngeal paralysis and 24 with vocal fold scarring) underwent vocal fold fat injection. Fat was harvested by low-pressure liposuction and then processed by centrifugation. Refined fat aliquots were placed in the vocal fold and paraglottic space in multiple tunnels to enhance graft neovascularization...
November 1, 2017: Otolaryngology—Head and Neck Surgery
https://www.readbyqxmd.com/read/29158895/a-journey-through-liposuction-and-liposculture-review
#6
REVIEW
Elisa Bellini, Michele P Grieco, Edoardo Raposio
Introduction: Nowadays, liposuction is the most frequently performed aesthetic surgery procedure in Western Countries. This technique has had rapid development since the 1970s, when it was experimented for the first time by A. and G. Fischer. It is currently widely used in clinical practice for many different situations in aesthetic, reconstructive and functional fields. Materials and methods: This review aims to describe the historical evolution of liposuction by analyzing the transformation of the method in function of the introduction of innovative ideas or instruments...
December 2017: Annals of Medicine and Surgery
https://www.readbyqxmd.com/read/29145587/paradoxical-adipose-hypertrophy-pah-after-cryolipolysis
#7
Nathaniel Stroumza, Nelly Gauthier, Patricia Senet, Philippe Moguelet, Raphael Nail Barthelemy, Michael Atlan
Background: Cryolipolysis is a minimally invasive technique used to decrease local adipose tissue by thermal cooling. Paradoxical adipose hypertrophy (PAH) is a rare complication of cryolipolysis with important aesthetic consequences. Objectives: The objective of this study was to describe four cases of PAH after a cryolipolysis treatment. Methods: Between January 2014 and January 2017, all patients who had undergone a cryolipolysis treatment in a single center were reviewed...
November 14, 2017: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/29131778/adventures-and-misadventures-in-plastic-surgery-and-soft-tissue-implants
#8
Dana J Lin, Tony T Wong, Gina A Ciavarra, Jonathan K Kazam
Soft-tissue augmentation and implants are increasingly seen by the radiologist as more techniques emerge for a variety of indications and locations. Some surgical and implant procedures are performed for purely cosmetic reasons in otherwise healthy patients seeking to improve their body image, and some are performed for reconstruction after cancer or other chronic illnesses. Abdominoplasty, liposuction, and autologous fat grafting can be performed for abdominal and gluteal contouring. Injection of liquid injectable silicone has historically been fraught with legal issues, although it continues to be used for augmentation in a variety of anatomic locations...
November 2017: Radiographics: a Review Publication of the Radiological Society of North America, Inc
https://www.readbyqxmd.com/read/29129587/thighplasty-improving-aesthetics-through-revival-of-the-medial-horizontal-procedure-a-safe-and-scar-saving-option
#9
Karl Schwaiger, Elisabeth Russe, Klemens Heinrich, Florian Ensat, Gernot Steiner, Gottfried Wechselberger, Michaela Hladik
INTRODUCTION: Thighplasty is a common bodycontouring procedure, but also associated with a high complication rate. The purpose of this study was to access the outcome of the medial horizontal thigh lift as it is a common surgical technique regarding thigh deformity correction performed at the authors' department. Surgical keysteps, clinical applications, advantages and disadvantages of the procedure are shown. Postoperative evaluation took place with special focus on individual patient satisfaction...
October 25, 2017: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
https://www.readbyqxmd.com/read/29122499/micro-autologous-fat-transplantation-for-rejuvenation-of-the-dorsal-surface-of-the-aging-hand
#10
Lin Yun-Nan, Huang Shu-Hung, Lin Tsung-Ying, Chou Chih-Kang, Huang Yu-Hao, Takahashi Hidenobu, Lai Chung-Sheng, Lin Sin-Daw, Lin Tsai-Ming
BACKGROUND: Rejuvenation of the dorsal surface of the hand for various conditions, such as cutaneous dyschromia, fine wrinkling, skin laxity, visible extensor tendons, and varicosities appear vital to prevent wrist and digit flexion resulting from hiding of the aged appearance of the hand. Numerous fillers have been applied to re-contour the aging dorsal surface of the hand and the results were variable. The micro-autologous fat transplantation (MAFT) technique, proposed by Lin et al...
October 6, 2017: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
https://www.readbyqxmd.com/read/29113649/endoscopic-sentinel-lymph-node-biopsy-and-endoscopic-axillary-lymphadenectomy-without-liposuction-in-patients-with-early-stage-breast-cancer
#11
Jing Fang, Li Ma, Yong-Hui Zhang, Zheng-Jun Yang, Yue Yu, Xu-Chen Cao
BACKGROUND: Our purpose was to present a new method of endoscopic sentinel lymph node biopsy (ESLNB) and endoscopic axillary lymphadenectomy (EALND) without liposuction for treating early-stage breast cancer, and compare results with traditional open dissection. METHODS: The medical records of patients with early-stage breast cancer who underwent EALND/ESLNB without liposuction or traditional open dissection between March 2015 and September 2016 were retrospectively reviewed...
December 2017: Surgical Oncology
https://www.readbyqxmd.com/read/29113429/endophilin-a2-a-potential-link-to-adiposity-and-beyond
#12
Assim A Alfadda, Reem M Sallam, Rukhsana Gul, Injae Hwang, Sojeong Ka
Adipose tissue plays a central role in regulating dynamic crosstalk between tissues and organs. A detailed description of molecules that are differentially expressed upon changes in adipose tissue mass is expected to increase our understanding of the molecular mechanisms that underlie obesity and related metabolic co-morbidities. Our previous studies suggest a possible link between endophilins (SH3Grb2 proteins) and changes in body weight. To explore this further, we sought to assess the distribution of endophilin A2 (EA2) in human adipose tissue and experimental animals...
November 6, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/29108550/liposuction-considerations-in-men
#13
REVIEW
Cheryl Karcher
Liposuction is the most popular cosmetic surgical procedure performed in men. Aside from traditional liposuction using the tumescent technique, several energy-assisted liposuction technologies have emerged and been proven safe and particularly beneficial for the male patient demographic. The differences in fat distribution and unique goals and emotional perspective in men need to be taken into account when performing liposuction, to ensure meeting the patient's needs and expectations for satisfactory results...
January 2018: Dermatologic Clinics
https://www.readbyqxmd.com/read/29104686/liposuction-assisted-lipoma-removal-option-or-alternative
#14
Igor Peev, Liljana Spasevska, Elizabeta Mirchevska, Smilja Tudzarova-Gjorgova
BACKGROUND: Lipomas are the most frequent soft -tissue tumors arising from adipose tissue. Traditionally, open surgery is a mainstay of their treatment. Recently, new treatment modalities emerge in order to decrease morbidity, to increase satisfaction rate in patents, but not to raise recurrence risk at the same time. AIM: The aim of this article is to present our experience with liposuction assisted lipoma removal in terms of efficacy, complications, risk of recurrence and patient satisfaction...
October 15, 2017: Open Access Macedonian Journal of Medical Sciences
https://www.readbyqxmd.com/read/29103962/corneal-stroma-enhancement-with-decellularized-stromal-laminas-with-or-without-stem-cell-recellularization-for-advanced-keratoconus
#15
Jorge L Alió Del Barrio, Mona El Zarif, Albert Azaar, Nehman Makdissy, Charbel Khalil, Walid Harb, Ibrahim El Achkar, Ziad Abdul Jawad, María P de Miguel, Jorge L Alió
PURPOSE: Phase-1 study to preliminarily evaluate the safety and efficacy of decellularized human corneal stromal lamina transplantation with or without autologous adipose derived adult stem cells (ADASC) recellularization within the corneal stroma of patients with advanced keratoconus. DESIGN: Phase one clinical trial METHODS: Femtosecond-assisted 120μm-thickness and 9mm-diameter laminas were obtained from the anterior stroma of human donor corneas and decellularized with a sodium dodecyl sulfate solution...
November 2, 2017: American Journal of Ophthalmology
https://www.readbyqxmd.com/read/29102952/comparison-of-the-viability-and-yield-of-adipose-derived-stem-cells-ascs-from-different-donor-areas
#16
Anastasios Tsekouras, Dimitrios Mantas, Diamantis I Tsilimigras, Demetrios Moris, Michael Kontos, George C Zografos
BACKGROUND: Adipose tissue contains variable amounts of stem cells that have recently attracted increased interest due to their strong proliferative and differentiation capacity. However, significant heterogeneity exists in terms of stem cell yield and viability profile among individuals as well as different donor areas. MATERIALS AND METHODS: Between June 2016 and July 2016, forty (40) women underwent outpatient cosmetic liposuction procedures conferring a total of 53 lipoaspirates; inner thigh (n=13), outer thigh (n=9), abdomen (n=9), waist (n=16) and inner knee (n=6)...
November 2017: In Vivo
https://www.readbyqxmd.com/read/29091562/liposuction-for-swelling-in-patients-with-lymphedema
#17
LETTER
Arin K Greene, Stephan D Voss, Reid A Maclellan
New England Journal of Medicine, Volume 377, Issue 18, Page 1788-1789, November 2017.
November 2, 2017: New England Journal of Medicine
https://www.readbyqxmd.com/read/29089717/can-liposuction-technique-be-used-as-a-treatment-method-in-cases-of-mercury-injections
#18
S S Shirol, Prathamesh Chandrapattan
No abstract text is available yet for this article.
October 2017: Indian Journal of Surgery
https://www.readbyqxmd.com/read/29080652/indications-and-controversies-in-total-breast-reconstruction-with-lipomodeling
#19
REVIEW
Emmanuel Delay, Andreea Carmen Meruta, Samia Guerid
Complete breast reconstruction with fat grafting is an appealing technique because it does not add scars or use flaps, is autologous, and has the secondary benefit of liposuction. It is a multiple stage surgery and usually requires balancing of the contralateral breast. After using this technique since 2001, the authors narrowed the indications, the ideal candidate being a patient with small breasts and enough donor sites for up to 4 or 5 lipomodeling sessions. Breast reconstruction with fat grafting alone in total breast reconstruction has very limited indications, because lipomodeling in combination with other autologous reconstruction gives very nice results...
January 2018: Clinics in Plastic Surgery
https://www.readbyqxmd.com/read/29076330/assessment-of-correlation-between-time-period-and-risk-of-fat-embolism-after-liposuction-animal-model
#20
Kwang Ryeol Lim, Jeong-Min Cho, Chung-Min Yoon, Keun-Cheol Lee, Sang-Yun Lee, Mi-Ha Ju
Background: Liposuction has become one of the most frequently performed operation among the aesthetic surgical field recently. Fat embolism syndrome after liposuction is overlooked or underestimated easily. Occasionally, fulminating fat embolism syndrome could be developed and lead to critical situation within 2-3 days after lipoplasty. Time period changes in amount of circulating fat particle and histology of major organs are not studied yet. Methods: This study was conducted using 18 male Sprague Dawley rats aged 12weeks and weighing 500-628g (562g on average)...
October 27, 2017: Archives of Plastic Surgery
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