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https://www.readbyqxmd.com/read/29028071/how-to-isolate-a-ready-to-use-adipose-derived-stem-cells-pellet-for-clinical-application
#1
E Raposio, N Bertozzi
Adipose-derived stem cells (ASCs) are multipotent mesenchymal stem cells (MSCs) that show definitive stem cell characteristics such as plastic adherence in culture, ability to maintain multipotency upon in vitro expansion, and self-renewal capacity. ASCs are particularly promising for use in regenerative medicine because they can be harvested easily from adipose tissue by standard liposuction, with minimal donor site morbidity. Since ASCs do not necessitate ex vivo expansion to obtain clinically significant cell numbers, it is critical to identify a standardized method that maximizes the number of ASCs collected...
September 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29025225/lymphatic-and-sensory-function-of-the-upper-limb-after-brachioplasty-in-post-bariatric-massive-weight-loss-patients
#2
Stefano Gentileschi, Maria Servillo, Gabriella Ferrandina, Marzia Salgarello
Background: Varied deformities of the upper arm are common after massive weight loss. Brachioplasty techniques have been successively modified to improve aesthetic outcomes and avoid complications, especially lymphedema and sensory damage. Objectives: The authors evaluated lymphatic drainage and sensory function of the upper limbs after brachioplasty performed with a double-ellipse marking technique, a medial incision, superficial undermining, and posterior arm liposuction...
October 1, 2017: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/29025212/use-of-freshly-isolated-human-adipose-stromal-cells-for-clinical-applications
#3
Ryan A Lockhart, Joel A Aronowitz, Severiano Dos-Anjos Vilaboa
The clinical use of adipose-derived cells is being explored very actively around the world for various human diseases. Adipose tissue is an abundant tissue source that can be easily harvested using liposuction. Human lipoaspirates contain a significant amount of mesenchymal stromal cells, as well as other progenitors and terminally differentiated cell types. This review covers the isolation of adipose stromal vascular fraction (SVF), the quality control and safety analysis of freshly isolated cell suspensions...
July 1, 2017: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/28972280/systematic-review-of-lymphovenous-anastomosis-lva-for-the-treatment-of-lymphedema
#4
REVIEW
Mario F Scaglioni, Duveken B Y Fontein, Michael Arvanitakis, Pietro Giovanoli
INTRODUCTION: Lymphedema is a chronic condition caused by the obstruction or impairment of lymphatic fluid transport resulting in irreversible skin fibrosis. Besides conservative therapy, surgical techniques for lymphedema including liposuction, lymphatico-lymphatic bypass, lymphovenous anastomosis (LVA), and vascularized lymph node transfer (VLNT) are options with increasing popularity in the recent past. In our review, we investigated the efficacy of LVA for the treatment of lymphedema...
October 3, 2017: Microsurgery
https://www.readbyqxmd.com/read/28956970/lymphedema-leads-to-fat-deposition-in-muscle-and-decreased-muscle-water-volume-after-liposuction-a-magnetic-resonance-imaging-study
#5
Mattias Hoffner, Pernilla Peterson, Sven Månsson, Håkan Brorson
BACKGROUND: Lymphedema leads to adipose tissue deposition. Water-fat magnetic resonance imaging (MRI) can quantify and localize fat and water. The presence of excess fat and excess water/muscle in the subfascial compartment of the lymphedematous limb has not been investigated before. The aim of this study was to investigate epifascial and subfascial fat and water contents in patients with chronic lymphedema before and after liposuction. METHODS AND RESULTS: Seven patients with arm lymphedema and six with leg lymphedema were operated on...
September 28, 2017: Lymphatic Research and Biology
https://www.readbyqxmd.com/read/28951714/the-use-of-high-tumescent-power-assisted-liposuction-in-the-treatment-of-madelung-s-collar
#6
Henryk Witmanowski, Łukasz Banasiak, Grzegorz Kierzynka, Jarosław Markowicz, Jerzy Kolasiński, Katarzyna Błochowiak, Paweł Szychta
No abstract text is available yet for this article.
August 2017: Postȩpy Dermatologii i Alergologii
https://www.readbyqxmd.com/read/28947428/fat-attacks-a-case-of-fat-embolisation-syndrome-postliposuction
#7
Adam Ali, George Theobald, Mohammed Asif Arshad
Liposuction is a procedure commonly performed in the UK usually with a low incidence of serious sequelae; however with larger patients and increased volumes of lipoaspirate, complications have been reported more frequently. One of the rare but very serious complications postliposuction is fat embolism syndrome (FES), a life-threatening condition difficult to diagnose and limited in treatment.The authors present the case of a 45-year-old woman who was admitted to the intensive care unit postelective liposuction for bilateral leg lipoedema...
September 25, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28946733/interstitial-granulomatous-dermatitis-with-granuloma-annulare-like-pattern-following-liposuction
#8
Indu Agarwal, Antoinette Thomas, Mohit Agarwal, Thomas Cibull
No abstract text is available yet for this article.
September 2017: Archives of Plastic Surgery
https://www.readbyqxmd.com/read/28946731/managing-complications-in-abdominoplasty-a-literature-review
#9
Pedro Vidal, Juan Enrique Berner, Patrick A Will
BACKGROUND: Abdominoplasty, with or without liposuction, is among the most frequently performed aesthetic procedures. Its main objective is to improve the body contour by means of excising redundant skin and fat tissue. Although abdominoplasty is considered a safe procedure with high satisfaction rates, intraoperative and postoperative complications can become a challenge for the surgical team. The aim of this article is to offer a synopsis of the most common complications arising after abdominoplasty, along with evidence-based guidelines about how to prevent and treat them...
September 2017: Archives of Plastic Surgery
https://www.readbyqxmd.com/read/28938366/-camouflaging-cleft-lip-scar-using-follicular-unit-extraction-hair-transplantation-combined-with-autologous-fat-grafting
#10
Osman Akdag, Nuh Evin, Mehtap Karamese, Zekeriya Tosun
BACKGROUND: The scar that occurs after cleft lip surgery poses a serious cosmetic problem. One of the methods used to solve this problem in adult male patients is hair transplantation. However, satisfactory results with this method cannot always be achieved due to possible graft loss. The corrective feature of fat grafting has been reported in many studies. The aim of this report is to share our results with hair transplantation combined with fat grafting in patients with cleft lip. METHODS: This study included 20 patients who had both a scar and alopecia in the cleft lip area...
September 15, 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28922324/biological-properties-and-therapeutic-value-of-cryopreserved-fat-tissue
#11
Takanobu Mashiko, Szu-Hsien Wu, Koji Kanayama, Rintaro Asahi, Takako Shirado, Masanori Mori, Ataru Sunaga, Shunji Sarukawa, Hirokazu Uda, Kotaro Yoshimura
BACKGROUND: Fat grafting frequently requires multiple treatments and thus repeated liposuction to achieve treatment goals. The purpose of this study is to evaluate whether cryopreservation of adipose tissue may facilitate future fat grafting. METHODS: Lipoaspirates were harvested from six adult females and preserved using two cryopreservation methods: 1) simple cooling to -80˚C (cryo-1); or 2) programmed cooling to -190˚C (cryo-2). Fresh fat, cryo-1 fat, and cryo-2 fat were analyzed both in vitro and in vivo...
September 14, 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28920163/correction-of-whistle-deformity-using-autologous-free-fat-grafting-first-results-of-a-pilot-study-and-review-of-the-literature
#12
Sven Holger Baum, Gunnar Rieger, Roman Pförtner, Christopher Mohr
PURPOSE: Whistle deformities are frequent sequelae after surgical correction of cleft lip, trauma, or tumor excision. The aim of this study was to examine the role of autologous free fat grafting in the reconstruction of whistle deformity. PATIENTS: Fifteen patients with whistle deformity were enrolled in this pilot study. The mean follow-up period was 19 months. Liposuction was done followed by the replantation of an average of 2.2 ml autologous fat per patient (range 0...
September 17, 2017: Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/28917626/ultrasound-assisted-liposuction-provides-a-source-for-functional-adipose-derived-stromal-cells
#13
Dominik Duscher, Zeshaan N Maan, Anna Luan, Matthias M Aitzetmüller, Elizabeth A Brett, David Atashroo, Alexander J Whittam, Michael S Hu, Graham G Walmsley, Khosrow S Houschyar, Arndt F Schilling, Hans-Guenther Machens, Geoffrey C Gurtner, Michael T Longaker, Derrick C Wan
BACKGROUND AIMS: Regenerative medicine employs human mesenchymal stromal cells (MSCs) for their multi-lineage plasticity and their pro-regenerative cytokine secretome. Adipose-derived mesenchymal stromal cells (ASCs) are concentrated in fat tissue, and the ease of harvest via liposuction makes them a particularly interesting cell source. However, there are various liposuction methods, and few have been assessed regarding their impact on ASC functionality. Here we study the impact of the two most popular ultrasound-assisted liposuction (UAL) devices currently in clinical use, VASER (Solta Medical) and Lysonix 3000 (Mentor) on ASCs...
September 13, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28916849/1000-consecutive-cases-of-laser-assisted-liposuction-utilizing-the-1440%C3%A2-nm-wavelength-nd-yag-laser-assessing-the-safety-and-efficacy
#14
Christopher T Chia, Mark G Albert, Sharon Del Vecchio, Spero J Theodorou
BACKGROUND: Liposuction remains one of the most popular aesthetic surgery procedures performed today, and it has undergone continuous refinements over the past four decades. Advancements in anesthesia, improvements in instrumentation, better understanding of fluid dynamics and the addition of energy to liposuction have led to better outcomes with improved safety and efficacy. OBJECTIVES: The purpose of this study is to review 1000 consecutive laser-assisted liposuction (LAL) cases utilizing the 1440 nm wavelength...
September 15, 2017: Aesthetic Plastic Surgery
https://www.readbyqxmd.com/read/28880460/cryopreserved-off-the-shelf-allogeneic-adipose-derived-stromal-cells-for-therapy-in-patients-with-ischemic-heart-disease-and-heart-failure-a-safety-study
#15
Jens Kastrup, Mandana Haack-Sørensen, Morten Juhl, Rebekka Harary Søndergaard, Bjarke Follin, Lisbeth Drozd Lund, Ellen Mønsted Johansen, Abbas Ali Qayyum, Anders Bruun Mathiasen, Erik Jørgensen, Steffen Helqvist, Jens Jørgen Elberg, Helle Bruunsgaard, Annette Ekblond
The present first-in-human clinical trial evaluated the safety and feasibility of a newly developed and cryopreserved Cardiology Stem Cell Centre adipose-derived stromal cell (CSCC_ASC) product from healthy donors for intramyocardial injection in ten patients with ischemic heart disease and ischemic heart failure (IHF). Batches of CSCC_ASC were isolated from three healthy donors by liposuction from abdominal adipose tissue. Adipose mesenchymal stromal cells were culture expanded in bioreactors without the use of animal constituents, cryopreserved, and stored in vials in nitrogen dry-storage containers until use...
September 7, 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28876554/surgical-anatomy-of-reduction-mammaplasty-a-historical-perspective-and-current-concepts
#16
A O Wamalwa, T Stasch, F W Nangole, S O Khainga
Reduction mammaplasty is the volumetric reduction in the bulk of the breast. Techniques have evolved from primarily reducing the breast bulk to reducing with emphasis on functional and aesthetic outcome. The deeper understanding of the surgical anatomy of the breast has guided this development. While Paulus Aegina (sixth century AD), Dieffenbach (1848) and Gaillard-Thomas (1882) set the pace in glandular reduction; Pousson (1897) and Dehner (1908) focused on breast ptosis. It took quite some time before the enigma of the vascularization to the nipple areolar complex could be solved...
March 2017: South African Journal of Surgery. Suid-Afrikaanse Tydskrif Vir Chirurgie
https://www.readbyqxmd.com/read/28876553/the-circumareolar-approach-to-gynecomastia-and-transgender-surgery-modifications-to-provide-correct-nipple-areolar-complex-position-and-shape
#17
T Jay, K Segwapa
BACKGROUND: In surgery for gynaecomastia, excision with minimal scarring and correct position of the nipple areolar complex (NAC) are key to a successful outcome. We report on a pilot study on the use of a circumareolar excision and the use of mathematical vector calculation to position the NAC and execute surgery and report on the outcomes. METHOD: From 2014 to 2015, the authors operated on 9 gynecomastia or transgender patients. Based on research performed by Gertrude Beer, Beckenstein and Shulman we designed a mathematical modification of the technique described by Persichetti to provide correct NAC positioning as well as aesthetically pleasing NAC shape...
March 2017: South African Journal of Surgery. Suid-Afrikaanse Tydskrif Vir Chirurgie
https://www.readbyqxmd.com/read/28870056/-application-of-autologous-fat-grafting-in-breast-reconstruction
#18
L Cai, X F Han, B Q Wang, F C Li
Objective: To observe the outcome of breast reconstruction with autologous fat grafting in the patients following treatment for breast cancer. Methods: The clinical data of 22 patients after breast cancer modified radical mastectomy with fat grafting for breast reconstruction from January 2012 to March 2015 at Department of Body Contouring and Liposuction Center of Plastic Surgery, Hospital of Peking Union Medical College were analyzed retrospectively. The age of 22 patients (all female) was 28 to 54 years...
September 1, 2017: Zhonghua Wai Ke za Zhi [Chinese Journal of Surgery]
https://www.readbyqxmd.com/read/28858400/evaluation-of-389-patients-following-free-flap-lower-extremity-reconstruction-with-respect-to-secondary-refinement-procedures
#19
Dimitra Kotsougiani, Juliana Platte, Amir K Bigdeli, Bernd Hoener, Thomas Kremer, Ulrich Kneser, Leila Harhaus
INTRODUCTION: Primary functional and aesthetic results after free-flap salvage of lower extremities are occasionally suboptimal. The objective of this study was to identify predictors for secondary refinement procedures such as liposuction, dermolipectomy, or direct excision. In addition, patient-reported long-term satisfaction with the final reconstructive result was analyzed. PATIENTS AND METHODS: All patients undergoing free-flap lower extremity salvage between January 2011 and July 2015 were included in a comparative study...
August 31, 2017: Microsurgery
https://www.readbyqxmd.com/read/28844130/proteomic-analysis-of-human-adipose-derived-stem-cells-during-small-molecule-chemical-stimulated-pre-neuronal-differentiation
#20
Jerran Santos, Bruce K Milthorpe, Benjamin R Herbert, Matthew P Padula
Background: Adipose derived stem cells (ADSCs) are acquired from abdominal liposuction yielding a thousand fold more stem cells per millilitre than those from bone marrow. A large research void exists as to whether ADSCs are capable of transdermal differentiation toward neuronal phenotypes. Previous studies have investigated the use of chemical cocktails with varying inconclusive results. Methods: Human ADSCs were treated with a chemical stimulant, beta-mercaptoethanol, to direct them toward a neuronal-like lineage within 24 hours...
August 31, 2017: International Journal of Stem Cells
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