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Reactive oxygen species,endothelial damage

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https://www.readbyqxmd.com/read/29222016/the-toxin-bjussulaao-ii-induces-oxidative-stress-and-dna-damage-upregulates-the-inflammatory-cytokine-genes-tnf-and-il6-and-downregulates-the-apoptotic-related-genes-bax-bcl2-and-rela-in-human-caco-2-cells
#1
A R T Machado, A F Aissa, D L Ribeiro, L C Hernandes, C S Machado, M L P Bianchi, S V Sampaio, L M G Antunes
Colorectal carcinoma is one of the most common cancers in adults. As chemotherapy, the first-choice treatment for colorectal carcinoma, is often infeasible due to acquired tumor resistance and several adverse effects. It is important to discover and explore new molecules with better therapeutic action. Snake venom toxins have shown promising results with high cytotoxicity against tumor cells, but their mechanisms of action remain unclear. Here we examined how BjussuLAAO-II, an L-amino acid oxidase isolated from Bothrops jararacussu snake venom, exerts cytotoxicity towards colorectal adenocarcinoma human cells (Caco-2) and in human umbilical vein endothelial cell line (HUVEC)...
December 5, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29200830/notoginsenoside-r1-attenuates-high-glucose-induced-endothelial-damage-in-rat-retinal-capillary-endothelial-cells-by-modulating-the-intracellular-redox-state
#2
Chunlan Fan, Yuan Qiao, Minke Tang
The aim of this study was to examine whether Notoginsenoside R1 (NR1) attenuates high glucose-induced cell damage in rat retinal capillary endothelial cells (RCECs) and to explore the mechanisms involved. The exposure of rat RCECs to high concentration of glucose (30 mM) for 72 h led to significant cytotoxicity, including decreased cell viability, reduced mitochondrial DNA copy number, increased lactate dehydrogenase release and elevated apoptosis. NR1, when present in the culture medium, markedly attenuated the high glucose-induced cytotoxicity in rat RCECs...
2017: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/29200735/therapeutic-effects-of-cyathula-officinalis-kuan-and-its-active-fraction-on-acute-blood-stasis-rat-model-and-identification-constituents-by-hplc-qtof-ms-ms
#3
Yanmei Cao, Cuicui Gu, Fangli Zhao, Yuanlin Tang, Xiaobing Cui, Le Shi, Li Xu, Lian Yin
Background: Cyathula officinalis Kuan is widely used in the clinics for the treatment of blood stasis in China. Objective: To evaluate the improving blood rheology and anti-inflammatory properties of C. officinalis Kuan extract (CO) and its active fraction (ACO) on acute blood stasis model Wistar rats and characterize the correlative constituents. Materials and Methods: CO at 0.26, 0.53, and 1.04 g/kg and ACO at 0.38, 0.75, and 1.5 g/kg were administered to acute blood stasis model Wistar rats for 3 days...
October 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29132816/deferiprone-inhibits-iron-overload-induced-tissue-factor-bearing-endothelial-microparticle-generation-by-inhibition-oxidative-stress-induced-mitochondrial-injury-and-apoptosis
#4
Shing Chan, Qizhou Lian, Mei-Pian Chen, Dan Jiang, Jolie T K Ho, Yiu-Fai Cheung, Godfrey C F Chan
Iron overload-induced cardiovascular toxicity is one of the most common causes of morbidity and mortality in beta-thalassemia major patients. We have previously shown that iron overload-induced systemic arterial changes characterized by endothelial dysfunction are associated with increased endothelial microparticle (EMP) release. In this study, we further demonstrate how EMP release is associated with iron-induced mitochondrial injury and apoptosis of endothelial cells. Iron increased the production of reactive oxygen species (ROS) and calcium influx into mitochondria [Ca(2+)]m...
November 10, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29130363/antioxidant-role-of-autophagy-in-maintaining-the-integrity-of-glomerular-capillaries
#5
Jun Matsuda, Tomoko Namba, Yoshitsugu Takabatake, Tomonori Kimura, Atsushi Takahashi, Takeshi Yamamoto, Satoshi Minami, Shinsuke Sakai, Ryuta Fujimura, Jun-Ya Kaimori, Isao Matsui, Takayuki Hamano, Yoko Fukushima, Keiko Matsui, Tomoyoshi Soga, Yoshitaka Isaka
Autophagy is a lysosomal degradation system by which cytosolic materials and damaged organelles are broken down into basic components. To explore the physiological role of autophagy in glomerular endothelial cells (GEnCs), we compared the autophagic flux among cells in the kidney under starvation. Inhibition of autophagy by chloroquine administration significantly increased the number of autophagosomes or autolysosomes in GEnCs and proximal tubular cells, but not in podocytes, suggesting that the GEnCs exhibit substantial autophagic activity...
November 13, 2017: Autophagy
https://www.readbyqxmd.com/read/29121093/fish-oil-omega-3-polyunsaturated-fatty-acids-attenuate-oxidative-stress-induced-dna-damage-in-vascular-endothelial-cells
#6
Chiemi Sakai, Mari Ishida, Hideo Ohba, Hiromitsu Yamashita, Hitomi Uchida, Masao Yoshizumi, Takafumi Ishida
OBJECTIVE: Omega-3 fatty acids, particularly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), likely prevent cardiovascular disease, however their mechanisms remain unclear. Recently, the role of DNA damage in atherogenesis has been receiving considerable attention. Here, we investigated the effects of EPA and DHA on DNA damage in vascular endothelial cells to clarify their antiatherogenic mechanisms. METHODS AND RESULTS: We determined the effect of EPA and DHA on H2O2-induced DNA damage response in human aortic endothelial cells...
2017: PloS One
https://www.readbyqxmd.com/read/29118911/fibroblast-growth-factor-21-delayed-endothelial-replicative-senescence-and-protected-cells-from-h2o2-induced-premature-senescence-through-sirt1
#7
Jinhua Yan, Jinli Wang, Huijin Huang, Yi Huang, Tao Mi, Cuntai Zhang, Le Zhang
Vascular aging is an independent risk factor for age-related diseases, including atherosclerosis. Fibroblast growth factor 21 (FGF21) has been widely recognized as a metabolic regulator that is elevated in response to caloric and nutritional restrictions. Recent studies have demonstrated its emerging role as a pro-longevity hormone, but its effects on the senescence of human umbilical vascular endothelial cells (HUVECs) remain unclear. In the present study, we explored the anti-senescence effects and underlying mechanism of FGF21 on HUVECs...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/29112633/2017-military-supplement-current-challenges-in-the-development-of-acellular-hemoglobin-oxygen-carriers-by-protein-engineering
#8
Andres S Benitez Cardenas, Premila P Samuel, John S Olson
This article reviews the key biochemical mechanisms that govern O2 transport, NO scavenging, and oxidative degradation of acellular hemoglobin and how these ideas have been used to try to develop strategies to engineer safer and more effective hemoglobin-based oxygen carriers (HBOCs). Significant toxicities due to acellular hemoglobin (Hb) have been observed after the administration of HBOCs or after the lysis of red cells, and include: (a) rapid clearance and kidney damage due to dissociation into dimers, haptoglobin binding, and macrophage activation; (b) early O2 release leading to decreased tissue perfusion in capillary beds; (c) interference with endothelial and smooth muscle signaling due to nitric oxide (NO) scavenging; (d) autooxidization of heme iron followed by production of reactive oxygen species; and (e) iron overload symptoms due to hemin loss, globin denaturation, iron accumulation, and further inflammation...
November 6, 2017: Shock
https://www.readbyqxmd.com/read/29112156/effects-of-lonomia-obliqua-venom-on-vascular-smooth-muscle-cells-contribution-of-nadph-oxidase-derived-reactive-oxygen-species
#9
João Alfredo Moraes, Genilson Rodrigues, Vany Nascimento-Silva, Mariana Renovato-Martins, Markus Berger, Jorge Almeida Guimarães, Christina Barja-Fidalgo
Envenomation caused by human contact with the caterpillar Lonomia is characterized by deleterious effects on coagulation and patency of blood vessels. The cellular effects induced by Lonomia obliqua venom highlights its capacity to activate endothelial cells, leading to a proinflammatory phenotype. Having more knowledge about the mechanisms involved in envenomation may contribute to better treatment. We aimed to evaluate the effects of Lonomia obliqua caterpillar bristle extract (LOCBE) on vascular smooth muscle cells (VSMC)...
November 7, 2017: Toxins
https://www.readbyqxmd.com/read/29108743/differentially-dna-methylation-changes-induced-in-vitro-by-traffic-derived-nanoparticulate-matter
#10
Xiaoning Lei, Joshua E Muscat, Bo Zhang, Xuyang Sha, Guangli Xiu
The cytotoxicity, apoptosis, reactive oxygen species (ROS) and DNA damage induced by the commercial diesel exhaust particulate matter (PM) (SRM2975) with concentrations ranging from 0.1μg·mL-1 to 20μg·mL-1 were assessed in human umbilical vein endothelial cells (HUVECs) in vitro. An epigenetics-wide investigation of DNA methylation profiles was also performed using a new methylation beadchip (Illumina 850K) to identify the health effects of traffic-derived nanoparticulate matter (nPM) with a concentration of 20μg·mL-1...
November 3, 2017: Toxicology
https://www.readbyqxmd.com/read/29094329/allopurinol-protects-human-glomerular-endothelial-cells-from-high-glucose-induced-reactive-oxygen-species-generation-p53-overexpression-and-endothelial-dysfunction
#11
Theodoros Eleftheriadis, Georgios Pissas, Georgia Antoniadi, Vassilios Liakopoulos, Ioannis Stefanidis
PURPOSE: Mitochondrial reactive oxygen species (ROS) overproduction in capillary endothelial cells is a prerequisite for the development of diabetic nephropathy. Inhibition of xanthine oxidase, another ROS generator, ameliorates experimental diabetic nephropathy. To test the hypothesis that the initial high glucose-induced ROS production by the mitochondria activates xanthine oxidase, which afterward remains as the major source of ROS, we cultured primary human glomerular endothelial cells (GEnC) under normal or high-glucose conditions, with or without the xanthine oxidase inhibitor allopurinol...
November 1, 2017: International Urology and Nephrology
https://www.readbyqxmd.com/read/29079519/n-acetylcysteine-effects-on-a-murine-model-of-chronic-critical-limb-ischemia
#12
Wallace Aguiar de Medeiros, Leandro Almeida da Silva, Dhébora Mozena Dall'Igna, Monique Michels, Andressa Manfredini, Juliano Dos Santos Cardoso, Larissa Constantino, Giselli Scaini, Francieli Vuolo, Emílio L Streck, Cristiane Ritter, Felipe Dal-Pizzol
During chronic limb ischemia, oxidative damage and inflammation are described. Besides oxidative damage, the decrease of tissue oxygen levels is followed by several adaptive responses. The purpose of this study was to determine whether supplementation with N-acetylcysteine (NAC) is effective in an animal model of chronic limb ischemia. Chronic limb ischemia was induced and animals were treated once a day for 30 consecutive days with NAC (30mg/kg). After this time clinical scores were recorded and soleus muscle was isolated and lactate levels, oxidative damage and inflammatory parameters were determined...
October 24, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29077881/interplay-among-h3k9-editing-enzymes-suv39h1-jmjd2c-and-src-1-drives-p66shc-transcription-and-vascular-oxidative-stress-in-obesity
#13
Sarah Costantino, Francesco Paneni, Agostino Virdis, Shafaat Hussain, Shafeeq Ahmed Mohammed, Giuliana Capretti, Alexander Akhmedov, Kevin Dalgaard, Sergio Chiandotto, J Andrew Pospisilik, Thomas Jenuwein, Marco Giorgio, Massimo Volpe, Stefano Taddei, Thomas F Lüscher, Francesco Cosentino
Aims: Accumulation of reactive oxygen species (ROS) promotes vascular disease in obesity, but the underlying molecular mechanisms remain poorly understood. The adaptor p66Shc is emerging as a key molecule responsible for ROS generation and vascular damage. This study investigates whether epigenetic regulation of p66Shc contributes to obesity-related vascular disease. Methods and results: ROS-driven endothelial dysfunction was observed in visceral fat arteries (VFAs) isolated from obese subjects when compared with normal weight controls...
October 25, 2017: European Heart Journal
https://www.readbyqxmd.com/read/29056472/contribution-of-lectin-like-oxidized-low-density-lipoprotein-receptor-1-and-lox-1-modulating-compounds-to-vascular-diseases
#14
REVIEW
Anja Hofmann, Coy Brunssen, Henning Morawietz
The lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) is the major receptor for binding and uptake of oxidized low-density lipoprotein (oxLDL) in endothelial cells. LOX-1 is also expressed in macrophages, smooth muscle cells and platelets. Following internalization of oxLDL, LOX-1 initiates a vicious cycle from activation of pro-inflammatory signaling pathways, thus promoting an increased reactive oxygen species formation and secretion of pro-inflammatory cytokines. LOX-1 plays a pivotal role in the development of endothelial dysfunction, foam cell and advanced lesions formation as well as in myocardial ischemia...
October 19, 2017: Vascular Pharmacology
https://www.readbyqxmd.com/read/29047084/ros-signaling-in-the-pathogenesis-of-acute-lung-injury-ali-and-acute-respiratory-distress-syndrome-ards
#15
Manuela Kellner, Satish Noonepalle, Qing Lu, Anup Srivastava, Evgeny Zemskov, Stephen M Black
The generation of reactive oxygen species (ROS) plays an important role for the maintenance of cellular processes and functions in the body. However, the excessive generation of oxygen radicals under pathological conditions such as acute lung injury (ALI) and its most severe form acute respiratory distress syndrome (ARDS) leads to increased endothelial permeability. Within this hallmark of ALI and ARDS, vascular microvessels lose their junctional integrity and show increased myosin contractions that promote the migration of polymorphonuclear leukocytes (PMNs) and the transition of solutes and fluids in the alveolar lumen...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29042481/the-matricellular-protein-tsp1-promotes-human-and-mouse-endothelial-cell-senescence-through-cd47-and-nox1
#16
Daniel N Meijles, Sanghamitra Sahoo, Imad Al Ghouleh, Jefferson H Amaral, Raquel Bienes-Martinez, Heather E Knupp, Shireen Attaran, John C Sembrat, Seyed M Nouraie, Mauricio M Rojas, Enrico M Novelli, Mark T Gladwin, Jeffrey S Isenberg, Eugenia Cifuentes-Pagano, Patrick J Pagano
Senescent cells withdraw from the cell cycle and do not proliferate. The prevalence of senescent compared to normally functioning parenchymal cells increases with age, impairing tissue and organ homeostasis. A contentious principle governing this process has been the redox theory of aging. We linked matricellular protein thrombospondin 1 (TSP1) and its receptor CD47 to the activation of NADPH oxidase 1 (Nox1), but not of the other closely related Nox isoforms, and associated oxidative stress, and to senescence in human cells and aged tissue...
October 17, 2017: Science Signaling
https://www.readbyqxmd.com/read/29024402/blueberry-metabolites-attenuate-lipotoxicity-induced-endothelial-dysfunction
#17
Divya Bharat, Rafaela Ramos Mororo Cavalcanti, Chrissa Petersen, Nathan Begaye, Brett Ronald Cutler, Marcella Melo Assis Costa, Renata Kelly Luna Gomes Ramos, Marina Ramos Ferreira, Youyou Li, Leena P Bharath, Emma Toolson, Paul Sebahar, Ryan E Looper, Thunder Jalili, Namakkal S Rajasekaran, Zhenquan Jia, J David Symons, Anandh Babu Pon Velayutham
SCOPE: Lipotoxicity-induced endothelial dysfunction is an important vascular complication associated with diabetes. Clinical studies support the vascular benefits of blueberry anthocyanins, but the underlying mechanism is unclear. We tested the hypothesis that metabolites of blueberry anthocyanins attenuate lipotoxicity-induced endothelial dysfunction. METHODS AND RESULTS: Human aortic endothelial cells (HAECs) were treated for 6 h with either: (i) the parent anthocyanins (malvidin-3-glucoside and cyanidin-3-glucoside); or (ii) the blueberry metabolites (hydroxyhippuric acid, hippuric acid, benzoic acid-4-sulfate, isovanillic acid-3-sulfate, and vanillic acid-4-sulfate), at concentrations known to circulate in humans following blueberry consumption...
October 12, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/29019365/blueberry-supplementation-attenuates-oxidative-stress-within-monocytes-and-modulates-immune-cell-levels-in-adults-with-metabolic-syndrome-a-randomized-double-blind-placebo-controlled-trial
#18
Anand R Nair, Nithya Mariappan, April J Stull, Joseph Francis
BACKGROUND: Blueberries (BB) have been shown to improve insulin sensitivity and endothelial function in obese and pre-diabetic humans, and decrease oxidative stress and inflammation, and ameliorate cardio-renal damage in rodents. This indicates that blueberries have a systemic effect and are not limited to a particular organ system. In order for blueberries to exert beneficial effects on the whole body, the mechanism would logically have to operate through modulation of cellular humoral factors...
October 11, 2017: Food & Function
https://www.readbyqxmd.com/read/28988496/6-gingerol-prevents-mehp-induced-dna-damage-in-human-umbilical-vein-endothelia-cells
#19
G Yang, X Gao, L Jiang, X Sun, X Liu, M Chen, X Yao, Q Sun, S Wang
Mono (2-ethylhexyl) phthalate (MEHP) is the principal metabolite of di (2-etylhexyl) phthalate, which is widely used as a plasticizer, especially in medical devices. MEHP has toxic effects on cardiovascular system. The aim of this study was to investigate the possibility that 6-gingerol may inhibit the oxidative DNA damage of MEHP in human umbilical vein endothelial cells (HUVECs) and the potential mechanism. The comet assay was used to monitor DNA strand breaks. We have shown that 6-gingerol significantly reduced the DNA strand breaks caused by MEHP...
November 2017: Human & Experimental Toxicology
https://www.readbyqxmd.com/read/28965081/sirt1-sirt3-axis-regulates-human-blood-brain-barrier-permeability-in-response-to-ischemia
#20
Tao Chen, Shu-Hui Dai, Xia Li, Peng Luo, Jie Zhu, Yu-Hai Wang, Zhou Fei, Xiao-Fan Jiang
Sirtuin1 (Sirt1) and Sirtuin3 (Sirt3) are two well-characterized members of the silent information regulator 2 (Sir2) family of proteins. Both Sirt1 and Sirt3 have been shown to play vital roles in resistance to cellular stress, but the interaction between these two sirtuins has not been fully determined. In this study, we investigated the role of Sirt1-Sirt3 axis in blood-brain barrier (BBB) permeability after ischemia in vitro. Human brain microvascular endothelial cells and astrocytes were co-cultured to model the BBB in vitro and oxygen and glucose deprivation (OGD) was performed to mimic ischemia...
September 22, 2017: Redox Biology
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