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https://www.readbyqxmd.com/read/29349493/biotic-elicitation-of-ginsenoside-metabolism-of-mutant-adventitious-root-culture-in-panax-ginseng
#1
Kim-Cuong Le, Wan-Taek Im, Kee-Yoeup Paek, So-Young Park
Biotic elicitation is an important biotechnological strategy for triggering the accumulation of secondary metabolites in adventitious root cultures. These biotic elicitors can be obtained from safe, economically important strains of bacteria found in the rhizosphere and fermented foods. Here, we assayed the effects of filtered cultures of five nitrogen-fixing bacteria and four types of fermentation bacteria on mutant adventitious Panax ginseng root cultures induced in a previous study by colchicine treatment...
January 18, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29315927/the-major-effect-qtl-csarn6-1-encodes-an-aaa-atpase-domain-containing-protein-that-is-associated-with-waterlogging-stress-tolerance-through-promoting-adventitious-root-formation
#2
Xuewen Xu, Jing Ji, Qiang Xu, Xiaohua Qi, Yiqun Weng, Xuehao Chen
In plants, the formation of hypocotyl-derived adventitious roots (AR) is an important morphological acclimation to waterlogging stress; however, its genetic basis remains fragmentary. Here, through combined use of bulked segregant analysis-based whole genome sequencing, SNP haplotyping and fine genetic mapping, we identified a candidate gene for a major-effect QTL ARN6.1 that was responsible for waterlogging tolerance due to increased AR formation in the cucumber line Zaoer-N. Through multiple lines of evidence, we show that the CsARN6...
January 8, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29305802/effect-of-water-cadmium-concentration-and-water-level-on-the-growth-performance-of-salix-triandroides-cuttings
#3
Xin Yao, Fengfeng Ma, Youzhi Li, Xiaohui Ding, Dongsheng Zou, Yandong Niu, Hualin Bian, Jiajun Deng
The growth performance of Salix triandroides cuttings at three water cadmium (Cd) concentrations (0, 20, and 40 mg L-1) and three water levels (- 40 cm, water level 40 cm below the soil surface; 0 cm, water level even with the soil surface; and 40 cm, water level 40 cm above soil surface) was investigated to evaluate its potential in phytoextraction strategies. Compared to cuttings in the - 40 or 0 cm water levels, cuttings in the 40 cm water level showed significantly lower biomass, height, and adventitious root length and significantly fewer leaves and adventitious roots...
January 5, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29284164/current-practices-in-the-management-of-postoperative-arterial-vasospasm-in-microsurgery
#4
Sergey Y Turin, Robert L Walton, Gregory A Dumanian, John B Hijjawi, John A LoGiudice, Mohammed Alghoul
BACKGROUND:  Postoperative microvascular arterial vasospasm is a rare clinical entity. There are no published management algorithms and also the pathophysiology of this phenomenon has not been elucidated. METHODS:  An email survey of American Society for Reconstructive Microsurgery (ASRM) and World Society for Reconstructive Microsurgery (WSRM) members regarding their experiences with postoperative arterial vasospasm was conducted, returning 116 responses. A comprehensive literature search was conducted regarding the current body of knowledge on this entity...
December 28, 2017: Journal of Reconstructive Microsurgery
https://www.readbyqxmd.com/read/29273862/heterorhizy-and-fine-root-architecture-of-rabbiteye-blueberry-vaccinium-virgatum-softwood-cuttings
#5
Takashi Baba, Satoshi Nakaba, Satoshi Noma, Ryo Funada, Takuya Ban
All fine root systems consist of individual fine roots. Individual roots have morphological, anatomical, and functional heterogeneity (heterorhizy). Heterorhizy plays crucial roles in plant ecosystems. However, in many species, the heterorhizy and fine root system architecture based on individual root units are unclear. This study investigated heterorhizy along the root system architecture of Vaccinium virgatum Ait (rabbiteye blueberry) softwood-cuttings (propagated from annual shoots in growing season) using protoxylem groups (PGs), a classification according to the number of protoxylem poles, as an indicator of individual root traits...
December 22, 2017: Journal of Plant Research
https://www.readbyqxmd.com/read/29249063/the-application-of-plant-in-vitro-cultures-in-cannabinoid-production
#6
REVIEW
Tomasz Wróbel, Mariola Dreger, Karolina Wielgus, Ryszard Słomski
Cannabinoids have considerable interest in the pharmaceutical industry. However, the production of medicines from hemp (Cannabis sativa L.) in most countries is restricted by law. Large-scale, field cultivation of hemp is difficult to control. Cannabinoid content in plants is variable and depends on multiple factors. Therefore, alternative methods of production have been investigated. The development of micropropagation techniques is a necessary step for genetic modification. Promising results have been obtained for certain narcotic genotypes...
December 16, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/29243424/-tissue-culture-of-callus-and-establishment-of-regeneration-system-of-tussilago-farfara-petiole
#7
Ji-Wen Ren, Ying Lei, Xiao-Ling Li
Young petiole of Tussilago farfara was used as the material to investigate the plant growth regulators which could influence in vitro culture and plant regeneration and to establish rapid propagation technique. The ideal sterilization method was that young petiole of T. farfara was sterilized with 75% ethanol for 30 s, and then transferred to saturated bleaching power supernatant for 15 min. The suitable medium for callus induction was MS+6-BA 3.0 mg•L⁻¹+2,4-D 2.0 mg•L⁻¹ with 96.2% induction rate...
October 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29242375/control-of-adventitious-root-architecture-in-rice-by-darkness-light-and-gravity
#8
Chen Lin, Margret Sauter
Rice is a semiaquatic plant that is well adapted to partial flooding. Rice stems develop adventitious root (AR) primordia at each node that slowly mature but emerge only when the plant gets flooded leading to the formation of a whole new secondary root system upon flooding. AR growth is induced by ethylene that accumulates in submerged plant tissues due to its lowered diffusion rate in water. Here we report that the architecture of the secondary root system in flooded rice plants is not only controlled by altered gas diffusion but also by gravity and light...
December 14, 2017: Plant Physiology
https://www.readbyqxmd.com/read/29194427/control-of-endogenous-auxin-levels-in-plant-root-development
#9
REVIEW
Damilola Olatunji, Danny Geelen, Inge Verstraeten
In this review, we summarize the different biosynthesis-related pathways that contribute to the regulation of endogenous auxin in plants. We demonstrate that all known genes involved in auxin biosynthesis also have a role in root formation, from the initiation of a root meristem during embryogenesis to the generation of a functional root system with a primary root, secondary lateral root branches and adventitious roots. Furthermore, the versatile adaptation of root development in response to environmental challenges is mediated by both local and distant control of auxin biosynthesis...
December 1, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29186815/characterization-of-the-transcriptome-and-gene-expression-of-tetraploid-black-locust-cuttings-in-response-to-etiolation
#10
(no author information available yet)
Etiolation (a process of growing plants in partial or complete absence of light) promotes adventitious root formation in tetraploid black locust (Robinia pseudoacacia L.) cuttings. We investigated the mechanism underlying how etiolation treatment promotes adventitious root formation in tetraploid black locust and assessed global transcriptional changes after etiolation treatment. Solexa paired-end sequencing of complementary DNAs (cDNAs) from control (non-etiolated, NE) and etiolated (E) samples resulted in 107,564 unigenes...
November 24, 2017: Genes
https://www.readbyqxmd.com/read/29160805/indole-3-butyric-acid-induces-ectopic-formation-of-metaxylem-in-the-hypocotyl-of-arabidopsis-thaliana-without-conversion-into-indole-3-acetic-acid-and-with-a-positive-interaction-with-ethylene
#11
Laura Fattorini, Federica Della Rovere, Eleonora Andreini, Marilena Ronzan, Giuseppina Falasca, Maria Maddalena Altamura
The role of the auxins indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) and of the auxin-interacting phytohormone ethylene, on the ectopic formation of primary xylem (xylogenesis in planta) is still little known. In particular, auxin/ethylene-target tissue(s), modality of the xylary process (trans-differentiation vs. de novo formation), and the kind of ectopic elements formed (metaxylem vs. protoxylem) are currently unknown. It is also unclear whether IBA may act on the process independently of conversion into IAA...
November 21, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29158642/insights-into-nuclear-dna-content-hydrogen-peroxide-and-antioxidative-enzyme-activities-during-transverse-thin-cell-layer-organogenesis-and-ex-vitro-acclimatization-of-malaxis-wallichii-a-threatened-medicinal-orchid
#12
Biswajit Bose, Suman Kumaria, Hiranjit Choudhury, Pramod Tandon
Malaxis wallichii (Lindl.) Deb, a small, perennial, monopodial, terrestrial orchid, is endemic to tropical Himalayas at an altitude of 1200-2000 m asl. The pseudobulbs are important ingredients of century old drug 'Ashtavarga' and a polyherbal energetic tonic 'Chyavanprash'. An efficient genetically stable in vitro propagation protocol using transverse thin cell layer culture system was established for M. wallichii. In the present report, meta-topolin alone proved to be three times more beneficial compared to other routinely used cytokinins in inducing highest number of shoot buds, plant height and growth of regenerated shoots...
October 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29138909/improvement-of-biosynthesis-and-accumulation-of-bioactive-compounds-by-elicitation-in-adventitious-root-cultures-of-polygonum-multiflorum
#13
Thanh-Tam Ho, Jong-Du Lee, Cheol-Seung Jeong, Kee-Yoeup Paek, So-Young Park
We examined the effects of abiotic (methyl jasmonate [MeJA] and salicylic acid [SA]) and biotic (yeast extract and chitosan) elicitors for improvement of bioactive compounds production on adventitious root cultures in Polygonum multiflorum. The application of yeast extract resulted in significantly (p ≤ 0.05) higher dry root biomass (9.98 g/L) and relative growth rate versus the control. Cultures treated with abiotic elicitors showed higher percentage of dry weight than the other samples. Low concentrations of all elicitors (50 μM MeJA and SA, and 50 mg/L yeast extract) improved secondary metabolite production except for chitosan, whose performance was worse than that of the control...
November 14, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29129002/histological-characterization-of-passiflora-pohlii-mast-root-tips-cryopreserved-using-the-v-cryo-plate-technique
#14
Mariela J Simão, Myriam Collin, Renata O Garcia, Elisabeth Mansur, Georgia Pacheco, Florent Engelmann
Cryopreservation stands out as the main strategy to ensure safe and cost efficient long-term conservation of plant germplasm, especially for biotechnological materials. However, the injuries associated with the procedure may result in structural damage and low recovery rates after cooling. Histological analysis provides useful information on the effects of osmotic dehydration, LN exposure, and recovery conditions on cellular integrity and tissue organization, allowing the determination of the critical steps of the cryopreservation protocol and, thus, the use of optimized treatments...
November 12, 2017: Protoplasma
https://www.readbyqxmd.com/read/29069281/melatonin-and-its-relationship-to-plant-hormones
#15
M B Arnao, J Hernández-Ruiz
Background: Plant melatonin appears to be a multi-regulatory molecule, similar to those observed in animals, with many specific functions in plant physiology. In recent years, the number of studies on melatonin in plants has increased significantly. One of the most studied actions of melatonin in plants is its effect on biotic and abiotic stress, such as that produced by drought, extreme temperatures, salinity, chemical pollution and UV radiation, among others. Scope: This review looks at studies in which some aspects of the relationship between melatonin and the plant hormones auxin, cytokinin, gibberellins, abscisic acid, ethylene, jasmonic acid and salicylic acid are presented...
October 21, 2017: Annals of Botany
https://www.readbyqxmd.com/read/29067033/events-associated-with-early-age-related-decline-in-adventitious-rooting-competence-of-eucalyptus-globulus-labill
#16
Márcio L Aumond, Artur T de Araujo, Camila F de Oliveira Junkes, Márcia R de Almeida, Hélio N Matsuura, Fernanda de Costa, Arthur G Fett-Neto
The development of adventitious roots is affected by several factors, including the age of the cutting donor plant, which negatively affects rooting capacity. Eucalyptus globulus quickly loses rooting capacity of cuttings as the donor plant ages, although the molecular and biochemical mechanisms behind this process are still unclear. To better understand the bases of rooting competence loss in E. globulus, the time required for a significant decline in rhizogenic ability without exogenous auxin was determined in microcuttings derived from donor plants of different ages after sowing...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29060511/performance-comparison-of-wavelet-based-denoising-methods-on-discontinuous-adventitious-lung-sounds
#17
Sezer Ulukaya, Gorkem Serbes, Yasemin P Kahya
Crackles and their time-domain characteristics provide important clues about different lung diseases. In this paper, we aim to de-noise synthetically produced crackles under various noise levels while preserving their information bearing parts which significantly affect crackle parameters. Classical wavelet based de-noising algorithms are deteriorated by sharp-sudden noise changes and produce Gibbs like fluctuations. On the other hand, total variation based algorithms, which are capable of alleviating the drawbacks of the classical wavelet based algorithms, are failed when dealing with piecewise-smooth signals like crackles and generate unwanted flat regions on the de-noised signals...
July 2017: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/29046689/an-updated-protocol-for-high-throughput-plant-tissue-sectioning
#18
Jonathan A Atkinson, Darren M Wells
Quantification of the tissue and cellular structure of plant material is essential for the study of a variety of plant sciences applications. Currently, many methods for sectioning plant material are either low throughput or involve free-hand sectioning which requires a significant amount of practice. Here, we present an updated method to provide rapid and high-quality cross sections, primarily of root tissue but which can also be readily applied to other tissues such as leaves or stems. To increase the throughput of traditional agarose embedding and sectioning, custom designed 3D printed molds were utilized to embed 5-15 roots in a block for sectioning in a single cut...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29044323/mini-incubators-improve-the-adventitious-rooting-performance-of-corymbia-and-eucalyptus-microcuttings-according-to-the-environment-in-which-they-are-conditioned
#19
Gilvano E Brondani, Leandro S DE Oliveira, Enéas R Konzen, André L L DA Silva, Jefferson L Costa
We addressed a major challenge in the in vitro clonal propagation of Corymbia citriodora, Eucalyptus urophylla and E. benthamii by using an ex vitro adventitious rooting strategy in a mini-incubator. Mini-incubators were placed in four environments for rooting. A shade house with no fogging system and a greenhouse with no ventilation but with a fogging environment had the best performance in terms of rooting, root growth and survival of microcuttings. Daily recording of the temperature within each mini-incubator in each environment allowed the verification of negative correlations between the maximum average temperature and the survival, adventitious rooting and root growth...
October 16, 2017: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/29036625/ospht1-8-a-phosphate-transporter-is-involved-in-auxin-and-phosphate-starvation-response-in-rice
#20
Hongfang Jia, Songtao Zhang, Lizhi Wang, Yongxia Yang, Hongying Zhang, Hong Cui, Huifang Shao, Guohua Xu
The responses of plants to auxin and phosphate (Pi) starvation are closely linked. However, the underlying mechanisms connecting the Pi starvation (-Pi) responses to auxin are largely unclear. Here, we show that OsPht1;8 (OsPT8), a phosphate transporter, functions in both the auxin and -Pi responses in rice (Oryza sativa L.) and tobacco (Nicotiana tabacum). The overexpression of OsPT8 (OsPT8-Oe) led to the loss of sensitivity to auxin and -Pi in adventitious roots, lateral roots, and root hairs in rice. The expression levels of OsPT8 and pOsPT8::GUS staining in roots, root-shoot junctions and leaves of rice were induced by IAA treatments...
November 2, 2017: Journal of Experimental Botany
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