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https://www.readbyqxmd.com/read/29143476/differences-in-innervated-neurons-of-the-internal-anal-sphincter-based-on-age-and-sex-a-histological-study
#1
Tomoaki Kaneko, Tetsuo Nemoto, Kimihiko Funahashi, Junichi Koike, Kazutoshi Shibuya, Hironori Kaneko
AIM: Previous studies have shown sex and age differences in anal sphincter function, but few morphological studies have focused on the quality and quantity of the nerves that control the sphincter muscles. The present study aimed to determine whether there are morphological and quantitative sex and age differences in the nerves in the conjoined longitudinal muscle. METHODS: This was a single-center, retrospective study using surgical specimens from 44 patients who underwent abdominoperineal resection between 2003 and 2012...
November 15, 2017: Geriatrics & Gerontology International
https://www.readbyqxmd.com/read/29138909/improvement-of-biosynthesis-and-accumulation-of-bioactive-compounds-by-elicitation-in-adventitious-root-cultures-of-polygonum-multiflorum
#2
Thanh-Tam Ho, Jong-Du Lee, Cheol-Seung Jeong, Kee-Yoeup Paek, So-Young Park
We examined the effects of abiotic (methyl jasmonate [MeJA] and salicylic acid [SA]) and biotic (yeast extract and chitosan) elicitors for improvement of bioactive compounds production on adventitious root cultures in Polygonum multiflorum. The application of yeast extract resulted in significantly (p ≤ 0.05) higher dry root biomass (9.98 g/L) and relative growth rate versus the control. Cultures treated with abiotic elicitors showed higher percentage of dry weight than the other samples. Low concentrations of all elicitors (50 μM MeJA and SA, and 50 mg/L yeast extract) improved secondary metabolite production except for chitosan, whose performance was worse than that of the control...
November 14, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29134459/risk-mitigation-in-preventing-adventitious-agent-contamination-of-mammalian-cell-cultures
#3
Masaru Shiratori, Robert Kiss
Industrial-scale mammalian cell culture processes have been contaminated by viruses during the culturing phase. Although the historical frequency of such events has been quite low, the impact of contamination can be significant for the manufacturing company and for the supply of the product to patients. This chapter discusses sources of adventitious agent contamination risk in a cell culture process, provides a semiquantitative assessment of such risks, and describes potential process barriers that can be used to reduce contamination risk...
November 14, 2017: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/29133148/adventitious-viruses-in-insect-cell-lines-used-for-recombinant-protein-expression
#4
REVIEW
Christoph Geisler, Donald L Jarvis
Insect cells are widely used for recombinant protein expression, typically as hosts for recombinant baculovirus vectors, but also for plasmid-mediated transient transfection or stable genetic transformation. Insect cells are used to express proteins for research, as well as to manufacture biologicals for human and veterinary medicine. Recently, several insect cell lines used for recombinant protein expression were found to be persistently infected with adventitious viruses. This has raised questions about how these infections might affect research performed using those cell lines...
November 10, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/29129002/histological-characterization-of-passiflora-pohlii-mast-root-tips-cryopreserved-using-the-v-cryo-plate-technique
#5
Mariela J Simão, Myriam Collin, Renata O Garcia, Elisabeth Mansur, Georgia Pacheco, Florent Engelmann
Cryopreservation stands out as the main strategy to ensure safe and cost efficient long-term conservation of plant germplasm, especially for biotechnological materials. However, the injuries associated with the procedure may result in structural damage and low recovery rates after cooling. Histological analysis provides useful information on the effects of osmotic dehydration, LN exposure, and recovery conditions on cellular integrity and tissue organization, allowing the determination of the critical steps of the cryopreservation protocol and, thus, the use of optimized treatments...
November 12, 2017: Protoplasma
https://www.readbyqxmd.com/read/29118111/differential-cell-matrix-mechanoadaptations-and-inflammation-drive-regional-propensities-to-aortic-fibrosis-aneurysm-or-dissection-in-hypertension
#6
M R Bersi, R Khosravi, A J Wujciak, D G Harrison, J D Humphrey
The embryonic lineage of intramural cells, microstructural organization of the extracellular matrix, local luminal and wall geometry, and haemodynamic loads vary along the length of the aorta. Yet, it remains unclear why certain diseases manifest differentially along the aorta. Toward this end, myriad animal models provide insight into diverse disease conditions-including fibrosis, aneurysm and dissection-but inherent differences across models impede general interpretations. We examined region-specific cellular, matrix, and biomechanical changes in a single experimental model of hypertension and atherosclerosis, which commonly coexist...
November 2017: Journal of the Royal Society, Interface
https://www.readbyqxmd.com/read/29111597/responses-of-an-adventitious-fast-growing-plant-to-photodynamic-stress-comparative-study-of-anionic-and-cationic-porphyrin-effect-on-arabidopsis-thaliana
#7
Mohammad Issawi, Damien Guillaumot, Vincent Sol, Catherine Riou
Antimicrobial Photodynamic Treatment based on the use of a photosensitizer to produce reactive oxygen species that induce cell death could be envisaged to fight against plant pathogens. For setting this strategy, we want to study how plants themselves respond to photodynamic treatment. In a previous work we showed that tomato plantlets were able to resist to photoactivated tetra (N-methylpyridyl) porphyrin (CP) or Zinc metalated form (CP-Zn). To enlarge our plant expertise related to exogenous porphyrins treatment and to further defend this approach, we studied how an adventice like Arabidopsis thaliana responded to exogenous supply of anionic and cationic porphyrins...
November 7, 2017: Physiologia Plantarum
https://www.readbyqxmd.com/read/29108502/colonic-adventitial-fibromuscular-dysplasia-a-nonspecific-arteriopathy-associated-with-hirschsprung-disease-and-other-obstructive-disorders
#8
Ameet I Thaker, Raj P Kapur
Background Smooth muscle differentiation ("adventitial fibromuscular dysplasia," AFD) was purported as specific to arteries in the transition zone of Hirschsprung disease (HSCR) patients. We investigated AFD in an HSCR population and controls and consider the pathogenesis and significance of the vascular pathology. Design Vascular histology in sections from colonic HSCR resections (n = 55) was compared with age- and site-matched controls with (n = 19) and without (n = 28) non-HSCR obstructive conditions...
January 1, 2017: Pediatric and Developmental Pathology
https://www.readbyqxmd.com/read/29097367/sex-chromosome-complement-defines-diffuse-versus-focal-angiotensin-ii-induced-aortic-pathology
#9
Yasir Alsiraj, Sean E Thatcher, Eric Blalock, Bradley Fleenor, Alan Daugherty, Lisa A Cassis
OBJECTIVE: Aortic pathologies exhibit sexual dimorphism, with aneurysms in both the thoracic and abdominal aorta (ie, abdominal aortic aneurysm [AAA]) exhibiting higher male prevalence. Women have lower prevalence of aneurysms, but when they occur, aneurysms progress rapidly. To define mechanisms for these sex differences, we determined the role of sex chromosome complement and testosterone on the location and progression of angiotensin II (AngII)-induced aortic pathologies. APPROACH AND RESULTS: We used transgenic male mice expressing Sry on an autosome to create Ldlr (low-density lipoprotein receptor)-deficient male mice with an XY or XX sex chromosome complement...
November 2, 2017: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/29093497/retinoic-acid-signaling-maintains-epithelial-and-mesenchymal-progenitors-in-the-developing-mouse-ureter
#10
Tobias Bohnenpoll, Anna-Carina Weiss, Maurice Labuhn, Timo H Lüdtke, M-O Trowe, Andreas Kispert
The differentiated cell types of the mature ureter arise from the distal ureteric bud epithelium and its surrounding mesenchyme. Uncommitted epithelial cells first become intermediate cells from which both basal and superficial cells develop. Mesenchymal progenitors give rise to separated layers of adventitial fibrocytes, smooth muscle cells and lamina propria fibrocytes. How progenitor expansion and differentiation are balanced is poorly understood. Here, we addressed the role of retinoic acid (RA) signaling in these programs...
November 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29089950/histogen-layers-contributing-to-adventitious-bud-formation-are-determined-by-their-cell-division-activities
#11
Tomoyuki Nabeshima, Soo-Jung Yang, Sho Ohno, Keita Honda, Ayumi Deguchi, Motoaki Doi, Fumi Tatsuzawa, Munetaka Hosokawa
Saintpaulia ionantha is propagated by adventitious buds in horticulture, and periclinal chimeral cultivars are usually difficult to propagate. However, some periclinal chimeral cultivars can be propagated with adventitious buds, and the mechanism of which has been unknown. Striped flower cultivars "Kaname," "Concord," and "Monique" were used to investigate what causes flower color separation in adventitious shoot-derived plants by tissue culture. These cultivars were revealed to have mutated flavonoid 3', 5' hydroxylase (SiF3'5'H), WDR1 (SiWDR1), or flavonoid 3 hydroxylase (SiF3H), respectively, in their L1 layer...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29078120/adventitial-tertiary-lymphoid-organ-classification-in-human-atherosclerosis
#12
Mohammadreza Akhavanpoor, Christian A Gleissner, Hamidreza Akhavanpoor, Felix Lasitschka, Andreas O Doesch, Hugo A Katus, Christian Erbel
BACKGROUND: Atherosclerosis is a chronic inflammatory disease of the arterial wall. Adjacent to lamina intima lesion progression, a cellular compound develops in the lamina adventitia, defined as tertiary lymphoid organs (TLO) in mice. But in human system, it remains unknown whether these adventitial cellular accumulations represent these highly organized immunological structures. PATIENTS AND METHODS: In this study, we investigated whether the adventitial cellular compounds represent TLOs in 72 human coronary artery samples by immunoenzyme staining...
September 7, 2017: Cardiovascular Pathology: the Official Journal of the Society for Cardiovascular Pathology
https://www.readbyqxmd.com/read/29075527/ultrasound-guided-percutaneous-aspiration-of-adventitial-cysts-in-the-occluded-popliteal-artery-clinical-results-and-mr-findings-at-5-year-follow-up
#13
Grzegorz Rosiak, Krzysztof Milczarek, Andrzej Cieszanowski, Olgierd Rowiński
Cystic adventitial disease is a rare disorder that occurs in peripheral arteries. Calf claudication caused by compression of the popliteal artery is a typical presentation of this disease. This is a report of two cases of occluded popliteal artery decompression by percutaneous ultrasound-guided cyst aspiration. In both cases, decompression of the artery was achieved with a significant decrease in the size of adventitial cysts and restoration of flow. Both patients reported complete resolution of symptoms and no calf pain 5 years after the procedure...
September 2017: Journal of Ultrasonography
https://www.readbyqxmd.com/read/29069281/melatonin-and-its-relationship-to-plant-hormones
#14
M B Arnao, J Hernández-Ruiz
Background: Plant melatonin appears to be a multi-regulatory molecule, similar to those observed in animals, with many specific functions in plant physiology. In recent years, the number of studies on melatonin in plants has increased significantly. One of the most studied actions of melatonin in plants is its effect on biotic and abiotic stress, such as that produced by drought, extreme temperatures, salinity, chemical pollution and UV radiation, among others. Scope: This review looks at studies in which some aspects of the relationship between melatonin and the plant hormones auxin, cytokinin, gibberellins, abscisic acid, ethylene, jasmonic acid and salicylic acid are presented...
October 21, 2017: Annals of Botany
https://www.readbyqxmd.com/read/29067033/events-associated-with-early-age-related-decline-in-adventitious-rooting-competence-of-eucalyptus-globulus-labill
#15
Márcio L Aumond, Artur T de Araujo, Camila F de Oliveira Junkes, Márcia R de Almeida, Hélio N Matsuura, Fernanda de Costa, Arthur G Fett-Neto
The development of adventitious roots is affected by several factors, including the age of the cutting donor plant, which negatively affects rooting capacity. Eucalyptus globulus quickly loses rooting capacity of cuttings as the donor plant ages, although the molecular and biochemical mechanisms behind this process are still unclear. To better understand the bases of rooting competence loss in E. globulus, the time required for a significant decline in rhizogenic ability without exogenous auxin was determined in microcuttings derived from donor plants of different ages after sowing...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29065676/analysis-of-cardiovascular-tissue-components-for-the-diagnosis-of-coronary-vulnerable-plaque-from-intravascular-ultrasound-images
#16
Ju Hwan Lee, Yoo Na Hwang, Ga Young Kim, Eun Seok Shin, Sung Min Kim
The purpose of this study was to characterize cardiovascular tissue components and analyze the different tissue properties for predicting coronary vulnerable plaque from intravascular ultrasound (IVUS) images. For this purpose, sequential IVUS image frames were obtained from human coronary arteries using 20 MHz catheters. The plaque regions between the intima and media-adventitial borders were manually segmented in all IVUS images. Tissue components of the plaque regions were classified into having fibrous tissue (FT), fibrofatty tissue (FFT), necrotic core (NC), or dense calcium (DC)...
2017: Journal of Healthcare Engineering
https://www.readbyqxmd.com/read/29060511/performance-comparison-of-wavelet-based-denoising-methods-on-discontinuous-adventitious-lung-sounds
#17
Sezer Ulukaya, Gorkem Serbes, Yasemin P Kahya
Crackles and their time-domain characteristics provide important clues about different lung diseases. In this paper, we aim to de-noise synthetically produced crackles under various noise levels while preserving their information bearing parts which significantly affect crackle parameters. Classical wavelet based de-noising algorithms are deteriorated by sharp-sudden noise changes and produce Gibbs like fluctuations. On the other hand, total variation based algorithms, which are capable of alleviating the drawbacks of the classical wavelet based algorithms, are failed when dealing with piecewise-smooth signals like crackles and generate unwanted flat regions on the de-noised signals...
July 2017: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/29052984/complex-structural-landscape-of-titanium-organophosphonates-isolation-of-structurally-related-ti4-ti5-and-ti6-species-and-mechanistic-insights
#18
Kamna Sharma, Rajendran Antony, Alok Ch Kalita, Sandeep Kumar Gupta, Paul Davis, Ramaswamy Murugavel
[Ti(acac)2(O(i)Pr)2] reacts with tert-butylphosphonic acid to yield a series of titanium organophosphonates such as tetranuclear [Ti4(acac)4(μ-O)2(μ-(t)BuPO3)2(μ-(t)BuPO3H)4]·2CH3CN (1), pentanuclear [Ti5(acac)5(μ-O)2(O(i)Pr)(μ-(t)BuPO3)4(μ-(t)BuPO3H)2] (2), hexanuclear [Ti6(acac)6(μ-O)2(O(i)Pr)2(μ-(t)BuPO3)6] (3), or [Ti6(acac)6(μ-O)3(O(i)Pr)(μ-(t)BuPO3)5(μ-(t)BuPO3H)]·2CH3CN (4). The isolation of each of these products in pure form depends on the molar ratio of the reactants or the solvent medium...
November 6, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/29047077/adventitial-fibroblast-nox4-expression-and-ros-signaling-in-pulmonary-arterial-hypertension
#19
Scott A Barman, David Fulton
Pulmonary arterial hypertension (PAH) is a progressive disease arising from remodeling and narrowing of pulmonary arteries (PA) resulting in high pulmonary arterial blood pressure and ultimately right ventricular failure. Elevated production of reactive oxygen species (ROS) by NADPH oxidase 4 (Nox4), a constitutively active enzyme, has been associated with oxygen sensing, vasomotor control, cellular proliferation, differentiation, migration, apoptosis, senescence, fibrosis, and angiogenesis. Further, elevated expression of Nox4 has been reported in a number of cardiovascular diseases, including atherosclerosis, hypertension, cardiac failure, ischemic stroke, and PAH...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29046689/an-updated-protocol-for-high-throughput-plant-tissue-sectioning
#20
Jonathan A Atkinson, Darren M Wells
Quantification of the tissue and cellular structure of plant material is essential for the study of a variety of plant sciences applications. Currently, many methods for sectioning plant material are either low throughput or involve free-hand sectioning which requires a significant amount of practice. Here, we present an updated method to provide rapid and high-quality cross sections, primarily of root tissue but which can also be readily applied to other tissues such as leaves or stems. To increase the throughput of traditional agarose embedding and sectioning, custom designed 3D printed molds were utilized to embed 5-15 roots in a block for sectioning in a single cut...
2017: Frontiers in Plant Science
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