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https://www.readbyqxmd.com/read/28345343/electronic-and-steric-optimization-of-fluorogenic-probes-for-biomolecular-imaging
#1
Wen Chyan, Henry R Kilgore, Brian Gold, Ronald T Raines
Fluorogenic probes are invaluable tools for spatiotemporal investigations within live cells. In common fluorogenic probes, the intrinsic fluorescence of a small-molecule fluorophore is masked by esterification until entry into a cell, where endogenous esterases catalyze the hydrolysis of the masking groups, generating fluorescence. The susceptibility of masking groups to spontaneous hydrolysis is a major limitation of these probes. Previous attempts to address this problem have incorporated auto-immolative linkers at the cost of atom economy and synthetic adversity...
March 27, 2017: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28343098/developmental-validation-of-qiagen-investigator-%C3%A2-24plex-qs-kit-and-investigator-%C3%A2-24plex-go-kit-two-6-dye-multiplex-assays-for-the-extended-codis-core-loci
#2
Melanie Kraemer, Anke Prochnow, Michael Bussmann, Mario Scherer, Ralf Peist, Carolyn Steffen
The original CODIS database based on 13 core STR loci has been overwhelmingly successful for matching suspects with evidence. In order to increase the power of discrimination, reduce the possibility of adventitious matches, and expand global data sharing, the CODIS Core Loci Working Group determined the expansion of the CODIS core loci to 20 STR plus three additional "highly recommended" loci (SE33, DY391, Amelogenin) Hares, 2015, 2012 [1,2]. The QIAGEN Investigator 24plex QS and Investigator 24plex GO! Kits are 6-dye multiplex assays that contain all markers of the expanded 23 CODIS core loci along with a unique internal performance control that is co-amplified with the STR markers...
March 12, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28338952/physiological-and-morphological-responses-to-permanent-and-intermittent-waterlogging-in-seedlings-of-four-evergreen-trees-of-temperate-swamp-forests
#3
Alejandra Zúñiga-Feest, Angela Bustos-Salazar, Fernanda Alves, Vanessa Martinez, Cecilia Smith-Ramírez, Joào Pereira
Waterlogging decreases a plant's metabolism, stomatal conductance (gs) and photosynthetic rate (A); however, some evergreen species show acclimation to waterlogging. By studying both the physiological and morphological responses to waterlogging, the objective of this study was to assess the acclimation capacity of four swamp forest species that reside in different microhabitats. We proposed that species (Luma apiculata [D.C.] Burret. and Drimys winteri J.R. et G. Forster.) abundant in seasonally and intermittently waterlogged areas (SIWA) would have a higher acclimation capacity than species abundant in the inner swamp (Blepharocalyx cruckshanksii [H et A...
March 14, 2017: Tree Physiology
https://www.readbyqxmd.com/read/28322699/markers-of-disease-severity-in-patients-with-spanish-influenza-in-the-japanese-armed-forces-1919-1920
#4
Koichiro Kudo, Toshie Manabe, Shinyu Izumi, Jin Takasaki, Yuji Fujikura, Akihiko Kawana, Kenji Yamamoto
We examined preserved medical charts of 470 Spanish influenza patients (8 with fatal cases) hospitalized at former army hospitals in Japan during 1919-1920. The following factors were associated with longer periods of hospitalization: adventitious discontinuous lung sounds, maximum respiration rate, continuation of high fever after hospital admission, and diphasic fever.
April 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28321650/the-small-auxin-up-rna-ossaur45-affects-auxin-synthesis-and-transport-in-rice
#5
Yan-Xia Xu, Meng-Zhu Xiao, Yan Liu, Jun-Liang Fu, Yi He, De-An Jiang
This research is the first to demonstrate that OsSAUR45 is involved in plant growth though affecting auxin synthesis and transport by repressing OsYUCCA and OsPIN gene expression in rice. Small auxin-up RNAs (SAURs) comprise a large multigene family and are rapidly activated as part of the primary auxin response in plants. However, little is known about the role of SAURs in plant growth and development, especially in monocots. Here, we report the biological function of OsSAUR45 in the model plant rice (Oryza sativa)...
March 20, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28315795/differential-auxin-transport-and-accumulation-in-the-stem-base-lead-to-profuse-adventitious-root-primordia-formation-in-the-aerial-roots-aer-mutant-of-tomato-solanum-lycopersicum-l
#6
F Mignolli, L Mariotti, P Picciarelli, M L Vidoz
The aerial roots (aer) mutant of tomato is characterized by a profuse and precocious formation of adventitious root primordia along the stem. We demonstrated that auxin is involved in the aer phenotype but ruled out higher auxin sensitivity of mutant plants. Interestingly, polar auxin transport was altered in aer, as young seedlings showed a reduced response to an auxin transport inhibitor and higher expression of auxin export carriers SlPIN1 and SlPIN3. An abrupt reduction in transcripts of auxin efflux and influx genes in older aer hypocotyls caused a marked deceleration of auxin transport in more mature tissues...
February 27, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28303391/transcriptional-sequencing-and-analysis-of-major-genes-involved-in-the-adventitious-root-formation-of-mango-cotyledon-segments
#7
Yun-He Li, Hong-Na Zhang, Qing-Song Wu, Gloria K Muday
A total of 74,745 unigenes were generated and 1975 DEGs were identified. Candidate genes that may be involved in the adventitious root formation of mango cotyledon segment were revealed. Adventitious root formation is a crucial step in plant vegetative propagation, but the molecular mechanism of adventitious root formation remains unclear. Adventitious roots formed only at the proximal cut surface (PCS) of mango cotyledon segments, whereas no roots were formed on the opposite, distal cut surface (DCS). To identify the transcript abundance changes linked to adventitious root development, RNA was isolated from PCS and DCS at 0, 4 and 7 days after culture, respectively...
March 16, 2017: Planta
https://www.readbyqxmd.com/read/28295951/c-1s-peak-of-adventitious-carbon-aligns-to-the-vacuum-level-dire-consequences-for-material-s-bonding-assignment-by-photoelectron-spectroscopy
#8
Grzegorz Greczynski, Lars Hultman
C 1s signal from ubiquitous carbon contamination on samples forming during air exposure, so called adventitious carbon (AdC) layers, is the most common binding energy (BE) reference in x-ray photoelectron spectroscopy studies. We demonstrate here, by using a series of transition metal nitride films with different AdC coverage, that the BE of C 1s peak E_B^F varies by as much as 1.44 eV. This is a factor of ten more than the typical resolvable difference between two chemical states of the same element, which makes BE referencing against the C 1s peak highly unreliable...
March 10, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/28286758/role-of-vitamin-d-in-uremic-vascular-calcification
#9
REVIEW
Yi-Chou Hou, Wen-Chih Liu, Cai-Mei Zheng, Jing-Quan Zheng, Tzung-Hai Yen, Kuo-Cheng Lu
The risk of cardiovascular death is 10 times higher in patients with CKD (chronic kidney disease) than in those without CKD. Vascular calcification, common in patients with CKD, is a predictor of cardiovascular mortality. Vitamin D deficiency, another complication of CKD, is associated with vascular calcification in patients with CKD. GFR decline, proteinuria, tubulointerstitial injury, and the therapeutic dose of active form vitamin D aggravate vitamin D deficiency and reduce its pleiotropic effect on the cardiovascular system...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28283956/evaluating-ultraviolet-sensitivity-of-adventitious-agents-in-biopharmaceutical-manufacturing
#10
REVIEW
Sarah M Meunier, Michael R Sasges, Marc G Aucoin
Incidents of contamination in biopharmaceutical production have highlighted the need to apply alternative or supplementary disinfection techniques. Ultraviolet (UV) irradiation is a well-established method for inactivating a broad range of microorganisms, and is therefore a good candidate as an orthogonal technique for disinfection. To apply UV as a safeguard against adventitious agents, the UV sensitivity of these target agents must be known so that the appropriate dose of UV may be applied to achieve the desired level of inactivation...
March 10, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28269092/detection-of-crackle-events-using-a-multi-feature-approach
#11
L Mendes, I M Vogiatzis, E Perantoni, E Kaimakamis, I Chouvarda, N Maglaveras, J Henriques, P Carvalho, R P Paiva
The automatic detection of adventitious lung sounds is a valuable tool to monitor respiratory diseases like chronic obstructive pulmonary disease. Crackles are adventitious and explosive respiratory sounds that are usually associated with the inflammation or infection of the small bronchi, bronchioles and alveoli. In this study a multi-feature approach is proposed for the detection of events, in the frame space, that contain one or more crackles. The performance of thirty-five features was tested. These features include thirty-one features usually used in the context of Music Information Retrieval, a wavelet based feature as well as the Teager energy and the entropy...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28269044/detection-of-patients-considering-observation-frequency-of-continuous-and-discontinuous-adventitious-sounds-in-lung-sounds
#12
Naoki Nakamura, Masaru Yamashita, Shoichi Matsunaga
We propose an improved approach for distinguishing between healthy subjects and patients with pulmonary emphysema by the use of one stochastic acoustic model for continuous adventitious sounds and another for discontinuous adventitious sounds. These models are able to represent the spectral features of the adventitious sounds for the detection of abnormal respiration. However, abnormal respiratory sounds with unclassifiable spectral features are present among the continuous and discontinuous adventitious sounds and mixing noises...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28268982/acoustic-evaluation-of-pirfenidone-on-patients-with-combined-pulmonary-fibrosis-emphysema-syndrome
#13
Sonia Charleston-Villalobos, Norma Castaneda-Villa, Ramon Gonzalez-Camarena, M Mejia-Avila, H Mateos-Toledo, Tomas Aljama-Corrales
The combined pulmonary fibrosis emphysema syndrome (CPFES) overall has a poor prognosis with a 5-year survival of 35-80%. Consequently, to evaluate possible positive effects on patients of novel agents as pirfenidone is relevant. However, the efficacy of pirfenidone in CPFES patients is still not well-known. In this study we propose an alternative to evaluate the effects of pirfenidone treatment on CPFES patients via acoustic information. Quantitative analysis of discontinuous adventitious lung sounds (DLS), known as crackles, has been promising to detect and characterize diverse pulmonary pathologies...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28257024/structure-of-a-pentamanganese-ii-phenoxide-cluster-with-a-central-five-coordinate-oxide-mn-ii-5-%C3%AE-oph-6-%C3%AE-3-oph-2-%C3%AE-5-o-py-6%C3%A2-py-py-is-pyridine
#14
Clifton R Hamilton, Michael J Zdilla
Polynuclear metal clusters frequently feature geometric structural features not common in traditional coordination chemistry. These structures are of particular interest to bioinorganic chemists studying metallocluster enzymes, which frequently possess remarkably unusual inorganic structures. The structure of the manganese cluster μ5-oxido-di-μ3-phenoxido-hexa-μ-phenoxido-hexakis(pyridine-κN)hexamanganese(II) pyridine monosolvate, [Mn5(C6H5O)8O(C5H5N)6]·C5H5N or Mn(II)5(μ-OPh)6(μ3-OPh)2(μ5-O)(Py)6·Py, containing an unusual trigonal bipyramidal central oxide, is described...
March 1, 2017: Acta Crystallographica. Section C, Structural Chemistry
https://www.readbyqxmd.com/read/28256674/piperazine-functionalized-c60-and-diiodine-or-iodine-monochloride-as-components-in-forming-supramolecular-assemblies
#15
Amineh Aghabali, Sharon Jun, Marilyn M Olmstead, Alan L Balch
The reaction of the piperazine mono-adduct, N(CH2CH2)2NC60, with diiodine produced well ordered, black crystals of (I2N(CH2CH2)2NI2)C60·2.884(C6H6)·0.116I2, which contains two nearly linear N-I-I units. Reaction of N(CH2CH2)2NC60 with iodine monochloride produced two materials: the dihalogen adduct, (ClIN(CH2CH2)2NICl)C60·2.3(CS2)·0.7(CH2Cl2), when crystallization occurred rapidly from carbon disulfide/dichloromethane solution or the salt, [(N(CH2CH2)2NH)C60(+)][ICl2(-)]·CS2, when crystallization happened more slowly from toluene/dichloromethane solution where hydrolysis of the iodine monochloride by adventitious water presumably occurred...
March 14, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28256503/the-developmental-and-environmental-regulation-of-gravitropic-setpoint-angle-in-arabidopsis-and-bean
#16
Suruchi Roychoudhry, Martin Kieffer, Marta Del Bianco, Che-Yang Liao, Dolf Weijers, Stefan Kepinski
Root and shoot branches are major determinants of plant form and critical for the effective capture of resources below and above ground. These branches are often maintained at specific angles with respect to gravity, known as gravitropic set point angles (GSAs). We have previously shown that the mechanism permitting the maintenance of non-vertical GSAs is highly auxin-dependent and here we investigate the developmental and environmental regulation of root and shoot branch GSA. We show that nitrogen and phosphorous deficiency have opposing, auxin signalling-dependent effects on lateral root GSA in Arabidopsis: while low nitrate induces less vertical lateral root GSA, phosphate deficiency results in a more vertical lateral root growth angle, a finding that contrasts with the previously reported growth angle response of bean adventitious roots...
March 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28253004/consistency-and-inconsistency-among-romantic-partners-over-time
#17
Paul W Eastwick, K Paige Harden, Jennifer A Shukusky, Taylor Anne Morgan, Samantha Joel
Theoretical perspectives on mating differentially emphasize whether (and why) romantic partner selection and maintenance processes derive from stable features of individuals (e.g., mate value, mate preferences, relationship aptitude) and their environments (e.g., social homogamy) rather than adventitious, dyad-specific, or unpredictable factors. The current article advances our understanding of this issue by assessing how people's actual romantic partners vary on constructs commonly assessed in evolutionary psychology (Study 1), sociology (Study 2), and close relationships (Study 3)...
March 2, 2017: Journal of Personality and Social Psychology
https://www.readbyqxmd.com/read/28250598/impacts-of-methyl-jasmonate-and-phenyl-acetic-acid-on-biomass-accumulation-and-antioxidant-potential-in-adventitious-roots-of-ajuga-bracteosa-wall-ex-benth-a-high-valued-endangered-medicinal-plant
#18
Sabahat Saeed, Huma Ali, Tariq Khan, Waqas Kayani, Mubarak Ali Khan
Ajuga bracteosa is a medicinally important plant globally used in the folk medicine against many serious ailments. In the present study, effects of two significant elicitors, methyl jasmonate (Me-J) and phenyl acetic acid (PAA) were studied on growth parameters, secondary metabolites production, and antioxidant potential in adventitious root suspension cultures of A. bracteosa. The results showed a substantial increase in biomass accumulation, exhibiting longer log phases of cultures growth in response to elicitor treatments, in comparison to control...
January 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/28242234/associations-between-common-carotid-artery-diameter-framingham-risk-score-and-cardiovascular-events
#19
M Kozakova, C Morizzo, S La Carrubba, I Fabiani, D Della Latta, J Jamagidze, D Chiappino, V Di Bello, C Palombo
BACKGROUND AND AIMS: Vascular biomarkers are associated with risk burden and are capable to predict the development of future cardiovascular (CV) events; yet, their additive predictive value over and above established risk algorithms seems to be only modest. The present study evaluated the cross-sectional associations between vascular biomarkers, 10-year Framingham risk (FR) and prevalent CV events in a population with a high prevalence of hypertension and diabetes. METHODS AND RESULTS: As many as 681 subjects (419 men, age = 60 ± 10 years, 282 diabetics, 335 hypertensives, mean FR score = 22...
January 12, 2017: Nutrition, Metabolism, and Cardiovascular Diseases: NMCD
https://www.readbyqxmd.com/read/28240555/poly-vinylidene-fluoride-pvdf-binder-degradation-in-li-o2-batteries-a-consideration-for-the-characterization-of-lithium-superoxide
#20
Joseph K Papp, Jason D Forster, Colin M Burke, Hyo Won Kim, Alan C Luntz, Robert M Shelby, Jeffrey J Urban, Bryan D McCloskey
We show that a common Li-O2 battery cathode binder, poly(vinylidene fluoride) (PVDF), degrades in the presence of reduced oxygen species during Li-O2 discharge when adventitious impurities are present. This degradation process forms products that exhibit Raman shifts (∼1133 and 1525 cm(-1)) nearly identical to those reported to belong to lithium superoxide (LiO2), complicating the identification of LiO2 in Li-O2 batteries. We show that these peaks are not observed when characterizing extracted discharged cathodes that employ poly(tetrafluoroethylene) (PTFE) as a binder, even when used to bind iridium-decorated reduced graphene oxide (Ir-rGO)-based cathodes similar to those that reportedly stabilize bulk LiO2 formation...
February 27, 2017: Journal of Physical Chemistry Letters
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