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Hirofumi Enomoto, Takuya Sensu, Kei Sato, Futoshi Sato, Thanai Paxton, Emi Yumoto, Koji Miyamoto, Masashi Asahina, Takao Yokota, Hisakazu Yamane
The plant hormone abscisic acid (ABA) and the jasmonic acid related-compound 12-oxo-phytodienoic acid (OPDA) play crucial roles in seed development, dormancy, and germination. However, a lack of suitable techniques for visualising plant hormones has restricted the investigation of their biological mechanisms. In the present study, desorption electrospray ionisation-imaging mass spectrometry (DESI-IMS), a powerful tool for visualising metabolites in biological tissues, was used to visualise ABA and OPDA in immature Phaseolus vulgaris L...
February 17, 2017: Scientific Reports
R Morando, E L L Baldin, P L Cruz, A L Lourenção
The soybean looper Chrysodeixis includens (Walker) (Lepidoptera: Noctuidae) is known as an important pest of leguminous plants worldwide. In Brazil, this pest species is gaining importance to producers of the common bean Phaseolus vulgaris L. (Fabaceae) because it limits field production of the crop. Chemical control is still the primary method of insect control. However, due to the possible harmful effects of pesticides to humans and the environment, alternative and less aggressive practices are being investigated...
February 16, 2017: Neotropical Entomology
Wen Hao Chen, Sheng Hui Yang, Zhao Hu Li, Xiao Xia Zhang, Xin Hua Sui, En Tao Wang, Wen Xin Chen, Wen Feng Chen
Two bacterial strains isolated from root nodules of soybean were characterized phylogenetically as members of a distinct group in the genus Ensifer based on 16S rRNA gene comparisons. They were also verified as a separated group by the concatenated sequence analyses of recA, atpD and glnII (with similarities ≤93.9% to the type strains for defined species), and by the average nucleotide identities (ANI) between the whole genome sequence of the representative strain CCBAU 251167(T) and those of the closely related strains in Ensifer glycinis and Ensifer fredii (90...
February 2, 2017: Systematic and Applied Microbiology
Pablo Peláez, Alejandrina Hernández-López, Georgina Estrada-Navarrete, Federico Sanchez
Agrobacterium rhizogenes is a pathogenic bacteria that causes hairy root disease by transferring bacterial DNA into the plant genome. It is an essential tool for industry and research due to its capacity to produce genetically modified roots and whole organisms. Here, we identified and characterized small RNAs generated from the transfer DNA (T-DNA) of A. rhizogenes in hairy roots of common bean (Phaseolus vulgaris). Distinct abundant A. rhizogenes T-DNA-derived small RNAs (ArT-sRNAs) belonging to several oncogenes were detected in hairy roots using high-throughput sequencing...
2017: Frontiers in Plant Science
Kelvin Kamfwa, Dongyan Zhao, James D Kelly, Karen A Cichy
Common bean (Phaseolus vulgaris L.) fixes atmospheric nitrogen (N2) through symbiotic nitrogen fixation (SNF) at levels lower than other grain legume crops. An understanding of the genes and molecular mechanisms underlying SNF will enable more effective strategies for the genetic improvement of SNF traits in common bean. In this study, transcriptome profiling was used to identify genes and molecular mechanisms underlying SNF differences between two common bean recombinant inbred lines that differed in their N-fixing abilities...
2017: PloS One
Monica Carvajal-Yepes, Leidy Zambrano, Juan M Bueno, Bodo Raatz, Wilmer J Cuellar
A copy of the complete genome of a novel bipartite begomovirus infecting common bean (Phaseolus vulgaris L.) in Colombia was obtained by rolling-circle amplification (RCA), cloned, and sequenced. The virus is associated with leaf crumple symptoms and significant yield losses in Andean and Mesoamerican beans. Such symptoms have been reported increasingly in Colombia since at least 2002, and we detected the virus in leaf material collected since 2008. Sequence analysis showed that the virus is a member of a distinct species, sharing 81% and 76% nucleotide (nt) sequence identity (in DNA-A and DNA-B, respectively) to other begomoviruses infecting common bean in the Americas...
February 10, 2017: Archives of Virology
Jugpreet Singh, Jose A Clavijo Michelangeli, Salvador A Gezan, Hyungwon Lee, C Eduardo Vallejos
Maternal control of seed size in the common bean provides an opportunity to study genotype-independent seed weight effects on early seedling growth and development. We set out to test the hypothesis that the early heterotrophic growth of bean seedlings is determined by both the relative amount of cotyledon storage reserves and the genotype of the seedling, provided the hybrid genotype could be fully expressed in the seedlings. The hypothesis was tested via comparison of seed weight and seedling growth phenotypes of small-seeded (wild, ~0...
2017: Frontiers in Plant Science
Lidia Chang-Sidorchuk, Heidy González-Alvarez, Jesús Navas-Castillo, Elvira Fiallo-Olivé, Yamila Martínez-Zubiaur
The common bean is a host for a large number of begomoviruses (genus Begomovirus, family Geminiviridae) in the New World. Based on the current taxonomic criteria established for the genus Begomovirus, two new members of this genus infecting common bean (Phaseolus vulgaris) in Cuba are herein reported. The cloned bipartite genomes, composed of DNA-A and DNA-B, showed the typical organization of the New World begomoviruses. We propose the names common bean severe mosaic virus and common bean mottle virus for the new begomovirus species...
February 6, 2017: Archives of Virology
Aregu Amsalu Aserse, Tanja Woyke, Nikos C Kyrpides, William B Whitman, Kristina Lindström
Rhizobium aethiopicum sp. nov. is a newly proposed species within the genus Rhizobium. This species includes six rhizobial strains; which were isolated from root nodules of the legume plant Phaseolus vulgaris growing in soils of Ethiopia. The species fixes nitrogen effectively in symbiosis with the host plant P. vulgaris, and is composed of aerobic, Gram-negative staining, rod-shaped bacteria. The genome of type strain HBR26(T) of R. aethiopicum sp. nov. was one of the rhizobial genomes sequenced as a part of the DOE JGI 2014 Genomic Encyclopedia project designed for soil and plant-associated and newly described type strains...
2017: Standards in Genomic Sciences
Tapan Kumar Mohanta, Pradeep Kumar, Hanhong Bae
BACKGROUND: Ca(2+) ion is a versatile second messenger that operate in a wide ranges of cellular processes that impact nearly every aspect of life. Ca(2+) regulates gene expression and biotic and abiotic stress responses in organisms ranging from unicellular algae to multi-cellular higher plants through the cascades of calcium signaling processes. RESULTS: In this study, we deciphered the genomics and evolutionary aspects of calcium signaling event of calmodulin (CaM) and calmodulin like- (CML) proteins...
February 3, 2017: BMC Plant Biology
Gerrit Wolters-Eisfeld, Udo Schumacher
Changes in glycosylation of the cancer cell glycocalyx are a hallmark of metastasizing cancers and critically contribute to distant metastasis. In this chapter we concentrate on two lectins capable of specifically binding tumor-associated glycans in cryostat or formalin-fixed, paraffin-embedded tissue sections derived from primary clinical material, genetically engineered mouse models with endogenous cancer formation or xenograft mouse models. The snail lectin of Helix pomatia (HPA) binds N-acetylgalactosamine (GalNAc) that is expressed among others as Tn antigen (O-linked GalNAc) in primary tumors and metastases in several human adenocarcinomas...
2017: Methods in Molecular Biology
Guadalupe Sosa-Valencia, Paulette S Romero-Pérez, Miguel Palomar, Alejandra A Covarrubias, José L Reyes
We recently described the activity of miR1514a in response to water deficit in Phaseolus vulgaris. Pvu-miR1514a targets a NAC transcription factor mRNA for cleavage and subsequently triggers NAC-derived phasiRNA formation. Here we show that accumulation and activity of miR1514a are also conserved in the model legume Medicago truncatula. Consistently, we identified Mtr-miR1514a and detected its increased accumulation in response to stress conditions, targeting a NAC TF mRNA for cleavage and triggering phasiRNA production...
February 2, 2017: Plant Signaling & Behavior
Rosalina García-Suárez, Luis A Verduzco-Rosas, Ma Cristina Del Rincón-Castro, John Délano-Frier, Jorge E Ibarra
AIMS: To demonstrate the ability of Bacillus thuringiensis to penetrate as spore-crystal complex to the internal tissues of bean plants, and keep its insecticidal activity. To test the vertical transmission of the spore-crystal complex in Arabidopsis thaliana. METHODS AND RESULTS: The experimental strain was transformed with the pMUTIN-gfp plasmid which labeled the spores of B. thuringiensis HD-73 with the GFP protein. Once the rhizosphere of the bean plants was inoculated with the labeled strain, the bacterium was recovered from leaves, stems, and petioles...
January 27, 2017: Journal of Applied Microbiology
Dai Cheng, Ruhua Wang, Chunling Wang, Lihua Hou
Aluminum (Al) has been linked to the development of some cardiovascular diseases and mung bean is a functional food with the ability to detoxify. We aimed to evaluate the preventive effect and possible underlying mechanisms of the mung bean polyphenol extract (MPE) on Al-induced cardiotoxicity. Control, AlCl3 (171.8 mg Al per kg body weight), MPE + AlCl3 (Al-treatment plus 200 mg MPE per kg body weight), and a group of MPE per se were used. Al intake induced a significant increase of serum CK and LDH activity and the level of Na(+), Ca(2+), malondialdehyde and advanced oxidation protein products in the AlCl3-treated rats' heart tissue...
January 27, 2017: Food & Function
Gonzalo Torres Tejerizo, Daniel Wibberg, Anika Winkler, Ernesto Ormeño-Orrillo, Esperanza Martínez-Romero, Karsten Niehaus, Alfred Pühler, Jörn Kalinowski, Antonio Lagares, Andreas Schlüter, Mariano Pistorio
Rhizobium tibeticum was originally isolated from root nodules of Trigonella archiducis-nicolai grown in Tibet, China. This species is also able to nodulate Medicago sativa and Phaseolus vulgaris The whole-genome sequence of the type strain, R. tibeticum CCBAU85039(T), is reported in this study.
January 26, 2017: Genome Announcements
Shengnan Zhang, Shanshan Yang, Wei Chen, Yong Chen, Mingjuan Zhang, Xinai Zhou, Guohua Fan, Fu Ying Feng
A Gram-strain-negative, rod-shaped, motile bacterium, designated MIM27T, was isolated from the sand of Desert Mu Us, China. The strain could grow at 4-45 °C (optimum, 37 °C), at pH 6.5-9.0 (optimum, 8.0) and in the presence of 0-3 % (w/v) NaCl (optimum, 0 %). Phylogenetic analysis on 16S rRNA gene sequence demonstrated that the strain was belonged to the genus Rhizobium, with the highest similarity (96.5 %) to Rhizobium pakistanensis BN-19T. Analysis of nitrogen fixation gene nifH and three housekeeping gene sequences of recA, atpD and glnII were also showed that strain MIM27T was mostly related to the recognized species of the genus Rhizobium but the similarities were low ( 90...
January 23, 2017: International Journal of Systematic and Evolutionary Microbiology
Miao Li, Qin Liu, Yajuan Cui, Dong Li, Hexiang Wang, Tzi Bun Ng
A 17.5-kDa trypsin inhibitor was purified from Phaseolus vulgaris cv. "gold bean" with an isolation protocol including ion exchange chromatography on DEAE-cellulose (Diethylaminoethyl-cellulose), affinity chromatography on Affi-gel blue gel, ion exchange chromatography on SP-sepharose (Sulfopropyl-sepharose), and gel filtration by FPLC (Fast protein liquid chromatography) on Superdex 75. It dose-dependently inhibited trypsin with an IC50 value of 0.4 μM, and this activity was reduced in the presence of dithiothreitol in a dose- and time-dependent manner, signifying the importance of the disulfide linkage to the activity...
January 23, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Chaeyoung Lee, Dongwoon Yu, Hong-Kyu Choi, Ryan W Kim
The Fabaceae (legume family) is the third largest and the second of agricultural importance among flowering plant groups. In this study, we report the reconstruction of a composite comparative map composed of ten legume genomes, including seven species from the galegoid clade (Medicago truncatula, Medicago sativa, Lens culinaris, Pisum sativum, Lotus japonicus, Cicer arietinum, Vicia faba) and three species from the phaseoloid clade (Vigna radiata, Phaseolus vulgaris, Glycine max). To accomplish this comparison, a total of 209 cross-species gene-derived markers were employed...
2017: Genes & Genomics
Sofie Van Holle, Pierre Rougé, Els J M Van Damme
BACKGROUND AND AIMS: The Nictaba family groups all proteins that show homology to Nictaba, the tobacco lectin. So far, Nictaba and an Arabidopsis thaliana homologue have been shown to be implicated in the plant stress response. The availability of more than 50 sequenced plant genomes provided the opportunity for a genome-wide identification of Nictaba-like genes in 15 species, representing members of the Fabaceae, Poaceae, Solanaceae, Musaceae, Arecaceae, Malvaceae and Rubiaceae. Additionally, phylogenetic relationships between the different species were explored...
January 12, 2017: Annals of Botany
Mingli Chen, Jing Wu, Lanfen Wang, Nitin Mantri, Xiaoyan Zhang, Zhendong Zhu, Shumin Wang
Anthracnose is a destructive disease of the common bean (Phaseolus vulgaris L.). The Andean cultivar Hongyundou has been demonstrated to possess strong resistance to anthracnose race 81. To study the genetics of this resistance, the Hongyundou cultivar was crossed with a susceptible genotype Jingdou. Segregation of resistance for race 81 was assessed in the F2 population and F2:3 lines under controlled conditions. Results indicate that Hongyundou carries a single dominant gene for anthracnose resistance. An allele test by crossing Hongyundou with another resistant cultivar revealed that the resistance gene is in the Co-1 locus (therefore named Co-1HY)...
2017: PloS One
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