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https://www.readbyqxmd.com/read/29766514/effect-of-catalytic-subunit-phosphorylation-on-the-properties-of-snrk1-from-phaseolus-vulgaris-embryos
#1
Esther Zúñiga-Sánchez, Rogelio Rodríguez-Sotres, Patricia Coello, Eleazar Martínez-Barajas
Legume seed development represents a high demand for energy and metabolic resources to support the massive synthesis of starch and proteins. However, embryo growth occurs in an environment with reduced O2 that forces the plant to adapt its metabolic activities to maximize efficient energy use. SNF1-related protein kinase1 (SnRK1) is a master metabolic regulator needed for cells adaptation to conditions that reduce energy availability, and its activity is needed for the successful development of seeds. In bean embryo extracts, SnRK1 can be separated by anion exchange chromatography into two pools: one where the catalytic subunit is phosphorylated (SnRK1-p) and another with reduced phosphorylation (SnRK1-np)...
May 16, 2018: Physiologia Plantarum
https://www.readbyqxmd.com/read/29760688/dissecting-the-acid-stress-response-of-rhizobium-tropici-ciat-899
#2
Julio Guerrero-Castro, Luis Lozano, Christian Sohlenkamp
Rhizobium tropici CIAT899 is a nodule-forming α-proteobacterium displaying intrinsic resistance to several abiotic stress conditions such as low pH and high temperatures, which are common in tropical environments. It is a good competitor for Phaseolus vulgaris (common bean) nodule occupancy at low pH values, however little is known about the genetic and physiological basis of the tolerance to acidic conditions. To identify genes in R. tropici involved in pH stress response we combined two different approaches: (1) A Tn 5 mutant library of R...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29754058/plant-and-soil-responses-to-hydrothermally-converted-sewage-sludge-sewchar
#3
Tatiane Medeiros Melo, Michael Bottlinger, Elke Schulz, Wilson Mozena Leandro, Adelmo Menezes de Aguiar Filho, Hailong Wang, Yong Sik Ok, Jörg Rinklebe
This study compared the effects of sewchar and mineral fertilizer on plant responses in beans (Phaseolus vulgaris, var. "Jalo precoce") and soil properties in a pot experiment in a completely randomized design with two harvests. The initial treatments consisted of a control, sewchar doses of 4, 8, 16 and 32 Mg ha-1 and mineral fertilizer (30 mg N, 90 mg P2 O5 and 60 mg K2 O kg-1 ). The treatments (4 replications each) were fertilized with 135 mg P2 O5  kg-1 at the second harvest. The sewchar application rates correlated positively with the CEC, the water holding capacity, the availability of Zn, Ca, Fe, Cu, and P, and the concentrations of nitrate, ammonium, total N, total organic carbon and hot water extractable carbon...
May 2, 2018: Chemosphere
https://www.readbyqxmd.com/read/29752522/meta-qtl-analysis-of-seed-iron-and-zinc-concentration-and-content-in-common-bean-phaseolus-vulgaris-l
#4
Paulo Izquierdo, Carolina Astudillo, Matthew W Blair, Asif M Iqbal, Bodo Raatz, Karen A Cichy
Twelve meta-QTL for seed Fe and Zn concentration and/or content were identified from 87 QTL originating from seven population grown in sixteen field trials. These meta-QTL include 2 specific to iron, 2 specific to zinc and 8 that co-localize for iron and zinc concentrations and/or content. Common bean (Phaseolus vulgaris L.) is the most important legume for human consumption worldwide and it is an important source of microelements, especially iron and zinc. Bean biofortification breeding programs develop new varieties with high levels of Fe and Zn targeted for countries with human micronutrient deficiencies...
May 11, 2018: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/29737621/effect-of-elevated-co-2-on-the-interaction-between-invasive-thrips-frankliniella-occidentalis-and-its-host-kidney-bean-phaseolus-vulgaris
#5
Lei Qian, Shuqi He, Xiaowei Liu, Zujin Huang, Fajun Chen, Furong Gui
BACKGROUND: Elevated CO2 can alter the leaf damage caused by insect herbivores. Frankliniella occidentalis (Pergande) is highly destructive invasive pest in crop production worldwide. To investigate how elevated CO2 affects F. occidentalis fed with Phaseolus vulgaris and in particularly, the interaction between plant defense and thrips anti-defense, nutrients content and antioxidant enzymes activity of P. vulgaris have been measured, as well as the detoxifying enzymes activity of adult thrips...
May 8, 2018: Pest Management Science
https://www.readbyqxmd.com/read/29705203/biocontrol-of-sclerotinia-sclerotiorum-lib-de-bary-on-common-bean-by-native-lipopeptide-producer-bacillus-strains
#6
Daniela C Sabaté, Carolina Pérez Brandan, Gabriela Petroselli, Rosa Erra-Balsells, M Carina Audisio
Bacillus sp. B19, Bacillus sp. P12 and B. amyloliquefaciens B14 were isolated from soils of Salta province, and PGPR properties on the common bean (Phaseolus vulgaris L.) cv. Alubia and antagonistic activity against Sclerotinia sclerotiorum were studied. It was determined that B19 and P12 increased crop germination potential (GP) from the common bean by 14.5% compared to control seeds; these strains also increased root length (10.4 and 15%, respectively) and stem length (20.2 and 30%, respectively) compared to the control; however, as for the B14 strain, no increases in growth parameters were detected...
June 2018: Microbiological Research
https://www.readbyqxmd.com/read/29697298/genetic-analysis-of-victorin-sensitivity-and-identification-of-a-causal-nlr-gene-in-phaseolus-vulgaris
#7
Jennifer Mae Lorang, Christina H Hagerty, Rian Lee, Phillip McClean, Thomas Wolpert
Cochliobolus victoria, the causal agent of Victoria blight, is pathogenic due to its production of a toxin called victorin. Victorin sensitivity in oats, barley, Brachypodium, and Arabidopsis has been associated with nucleotide-binding site leucine-rich repeat (NLR) genes, a class of genes known for conferring disease resistance. In this work we investigated the sensitivity of Phaseolus vulgaris to victorin. We found that victorin sensivity in Phaseolus vulgaris is a developmentally regulated, quantitative trait...
April 26, 2018: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/29686520/enhancement-of-yield-nutritional-and-nutraceutical-properties-of-two-common-bean-cultivars-following-the-application-of-seaweed-extract-ecklonia-maxima
#8
Anna Kocira, Michał Świeca, Sławomir Kocira, Urszula Złotek, Anna Jakubczyk
In the present study, application of Ecklonia maxima extract (Kelpak SL - a water soluble concentrate) was optimized and its impact on yield, nutraceutical and nutritional potential of Phaseolus vulgaris L. (var. Aura and Toska) was measured. The study was carried out in 2012 and 2013 in Poland. During the growing season, 0.2% and 0.4% solution of Kelpak SL was applied by single and double spraying of plants. These four treatments with Kelpak SL were compared with the control, where no biostimulator was applied...
March 2018: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/29680064/fermented-non-digestible-fraction-from-combined-nixtamalized-corn-zea-mays-l-cooked-common-bean-phaseolus-vulgaris-l-chips-modulate-anti-inflammatory-markers-on-raw-264-7-macrophages
#9
I Luzardo-Ocampo, R Campos-Vega, M L Cuellar-Nuñez, P A Vázquez-Landaverde, L Mojica, J A Acosta-Gallegos, G Loarca-Piña
Chronic non-communicable diseases (NCDs) are low-level inflammation processes affected by several factors including diet. It has been reported that mixed whole grain and legume consumption, e.g. corn and common bean, might be a beneficial combination due to its content of bioactive compounds. A considerable amount would be retained in the non-digestible fraction (NDF), reaching the colon, where microbiota produce short-chain fatty acids (SCFAs) and phenolic compounds (PC) with known anti-inflammatory effect...
September 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29677119/systematic-review-and-meta-analysis-of-a-proprietary-alpha-amylase-inhibitor-from-white-bean-phaseolus-vulgaris-l-on-weight-and-fat-loss-in-humans
#10
Jay Udani, Ollie Tan, Jhanna Molina
The aim of this meta-analysis was to examine the evidence for the effectiveness of a proprietary alpha-amylase inhibitor from white bean ( Phaseolus vulgaris L.) supplementation interventions in humans on modification of body weight and fat mass. A systematic literature search was performed using three databases: PubMed, the Cochrane collaboration, and Google Scholar. In addition, the manufacturer was contacted for internal unpublished data, and finally, the reference section of relevant original research and review papers were mined for additional studies...
April 20, 2018: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/29674106/biochemical-properties-of-a-bacterially-expressed-bowman-birk-inhibitor-from-rhynchosia-sublobata-schumach-meikle-seeds-and-its-activity-against-gut-proteases-of-achaea-janata
#11
Soundappan S Mohanraj, Sarada D Tetali, Nalini Mallikarjuna, Aparna Dutta-Gupta, Kollipara Padmasree
Crude proteinase inhibitors (CPIs) extracted from the seeds of Rhynchosia sublobata, a wild relative of pigeon pea showed pronounced inhibitory activity on the larval gut trypsin-like proteases of lepidopteran insect pest - Achaea janata. Consequently, a full-length cDNA of Bowman-Birk inhibitor gene (RsBBI1) was cloned from the immature seeds of R. sublobata. It contained an ORF of 360 bp encoding a 119-amino acid polypeptide (13.3 kDa) chain with an N-terminus signal sequence comprising of 22 amino acids...
April 16, 2018: Phytochemistry
https://www.readbyqxmd.com/read/29658968/comet-and-cytogenetic-tests-as-tools-for-evaluating-genomic-instability-in-seeds-of-oryza-sativa-l-and-phaseolus-vulgaris-l-from-gene-banks
#12
Alisson F Dantas, Renata M Lopes, Maria L Fascineli, Solange C B R José, Juliano G Pádua, Marcos A Gimenes, Cesar K Grisolia
This study aimed to assess the feasibility of comet and cytogenetic tests as tools for evaluating genomic instability in seeds of Oryza sativa L. (rice) and Phaseolus vulgaris (beans) L. from gene banks. Rice and beans were exposed to methyl methanesulfonate (MMS) as a reference DNA damaging agent. Seeds of two accessions of rice and beans were obtained from Embrapa Rice and Beans - Brazil. Seed groups were imbibed in three concentrations of MMS for three periods of time to carry out cytogenetic tests, and for one period for the comet test...
January 2018: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/29648948/recessive-resistance-to-bean-common-mosaic-virus-conferred-by-the-bc-1-and-bc-2-genes-in-common-bean-phaseolus-vulgaris-l-affects-long-distance-movement-of-the-virus
#13
Xue Feng, Gardenia Orellana, James Myers, Alexander V Karasev
Recessive resistance to Bean common mosaic virus (BCMV) in common bean (Phaseolus vulgaris L.) is governed by four genes that include one strain-nonspecific helper gene bc-u, and three strain-specific genes bc-1, bc-2, and bc-3. The bc-3 gene was identified as an eIF4E translation initiation factor gene mediating resistance through disruption of the interaction between this protein and the VPg protein of the virus. The mode of action of bc-1 and bc-2 in expression of BCMV resistance is unknown, although bc-1 gene was found to affect systemic spread of a related potyvirus, Bean common mosaic necrosis virus...
April 12, 2018: Phytopathology
https://www.readbyqxmd.com/read/29628099/protecting-the-photosynthetic-performance-of-snap-bean-under-free-air-ozone-exposure
#14
Lu Zhang, Yasutomo Hoshika, Elisa Carrari, Kent O Burkey, Elena Paoletti
Tropospheric ozone (O3 ) is a major air pollutant and causes serious injury to vegetation. To protect sensitive plants from O3 damage, several agrochemicals have been assessed, including cytokinin (e.g., kinetin, KIN) and ethylenediurea (EDU) with cytokinin-like activity. In higher plant, leaves are primarily injured by O3 and protective agrochemicals are often applied by leaf spraying. To our knowledge, the mitigating abilities of EDU and KIN have not been compared directly in a realistic setup. In the present research, impacts of elevated O3 (2× ambient O3 , 24hr per day, for 8days) on an O3 sensitive line (S156) of snap bean (Phaseolus vulgaris), which is often used for biomonitoring O3 pollution, were studied in a free air controlled exposure system...
April 2018: Journal of Environmental Sciences (China)
https://www.readbyqxmd.com/read/29626361/a-dicistronic-precursor-encoding-mir398-and-the-legume-specific-mir2119-co-regulates-csd1-and-adh1-mrnas-in-response-to-water-deficit
#15
Carlos De la Rosa, Alejandra Alicia Covarrubias, José Luis Reyes
Plant microRNAs are commonly encoded in transcripts containing a single microRNA precursor. Processing by DICER-LIKE 1 and associated factors results in the production of a small RNA, followed by its incorporation into an AGO-containing protein complex to guide silencing of an mRNA possessing a complementary target sequence. Certain microRNA loci contain more that one precursor stem-loop structure, thus encoding more than one microRNA in the same transcript. Here we describe a unique case where the evolutionary-conserved miR398a is encoded in the same transcript as the legume-specific miR2119...
April 6, 2018: Plant, Cell & Environment
https://www.readbyqxmd.com/read/29614780/metabolomics-and-transcriptomics-identify-multiple-downstream-targets-of-paraburkholderia-phymatum-%C3%AF-54-during-symbiosis-with-phaseolus-vulgaris
#16
Martina Lardi, Yilei Liu, Gaetano Giudice, Christian H Ahrens, Nicola Zamboni, Gabriella Pessi
RpoN (or σ54 ) is the key sigma factor for the regulation of transcription of nitrogen fixation genes in diazotrophic bacteria, which include α- and β-rhizobia. Our previous studies showed that an rpoN mutant of the β-rhizobial strain Paraburkholderia phymatum STM815T formed root nodules on Phaseolus vulgaris cv. Negro jamapa, which were unable to reduce atmospheric nitrogen into ammonia. In an effort to further characterize the RpoN regulon of P. phymatum , transcriptomics was combined with a powerful metabolomics approach...
April 1, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29614382/extended-exposure-duration-of-cultured-intestinal-epithelial-cell-monolayers-in-characterizing-hazardous-and-non-hazardous-proteins
#17
C Zimmermann, A D Eaton, B B Lanter, J Roper, B P Hurley, B Delaney
Recent studies suggest that human derived intestinal epithelial cells (IECs) cultured as polarized monolayers on Transwell® filters may respond differently when exposed to hazardous and non-hazardous proteins. This experimental platform was based on apical exposure of IEC monolayers to test proteins for 24 h followed by assessment of barrier integrity and cell viability. In this study, Caco-2 and T84 IEC polarized monolayers were evaluated for barrier integrity and cytotoxicity following exposure to hazardous and non-hazardous proteins for 24, 48 and 72 h...
March 31, 2018: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/29606739/effects-of-cordyceps-militaris-l-fr-fermentation-on-the-nutritional-physicochemical-functional-properties-and-angiotensin-i-converting-enzyme-inhibitory-activity-of-red-bean-phaseolus-angularis-willd-w-f-wight-flour
#18
Yu Xiao, Mingmei Sun, Qiuqin Zhang, Yulian Chen, Junqing Miao, Xin Rui, Mingsheng Dong
The effects of solid-state fermentation with Cordyceps militaris (L.) Fr. on the nutritional, physicochemical, and functional properties as well as angiotensin I converting enzyme (ACE) inhibitory activity of red bean ( Phaseolus angularis [Willd.] W.F. Wight.) flour were determined. Fermentation increased the amount of small peptides but significantly decreased large peptides. Fermentation also increased proteins and essential amino acids (by 9.31 and 13.89%, respectively) and improved the in vitro protein digestibility (6...
April 2018: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/29604085/identification-and-characterization-of-a-novel-carboxylesterase-from-phaseolus-vulgaris-for-detection-of-organophosphate-and-carbamates-pesticides
#19
Xiao Yang, Juan Dai, Sujuan Zhao, Rong Li, Tim Goulette, Xianggui Chen, Hang Xiao
BACKGROUND: Organophosphate and carbamate pesticide residues in food and the environment pose a great threat to human health, and have made the easy and rapid detection of these pesticide residues an important task. Discovering new enzyme sources from plants can help reduce the cost of large-scale applications of rapid pesticide detection via enzyme inhibition. RESULTS: Plant esterase from kidney bean was purified. Kidney bean esterase is identified as a carboxylesterase by substrate and inhibitor specificity tests and mass spectrometry identification...
March 31, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29594614/changes-in-plant-growth-and-seed-production-in-wild-lima-bean-in-response-to-herbivory-are-attenuated-by-parasitoids
#20
Maximilien A C Cuny, Johanna Gendry, Johnattan Hernández-Cumplido, Betty Benrey
Lima bean plants (Phaseolus lunatus) exhibit compensatory growth responses to herbivory. Among the various factors that have been identified to affect plant compensatory growth are the extent and type of tissue damage, the herbivore's feeding mode and the time of damage. Another factor that can greatly impact plant responses to herbivory, but has been largely ignored in previous studies, is the action of parasitoids. In most cases, parasitoids halt or slow down the development of herbivorous hosts, which, can result in decreased leaf damage, thereby affecting plant responses and ultimately plant fitness...
March 29, 2018: Oecologia
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