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https://www.readbyqxmd.com/read/27853303/novel-genomic-and-evolutionary-insight-of-wrky-transcription-factors-in-plant-lineage
#1
Tapan Kumar Mohanta, Yong-Hwan Park, Hanhong Bae
The evolutionarily conserved WRKY transcription factor (TF) regulates different aspects of gene expression in plants, and modulates growth, development, as well as biotic and abiotic stress responses. Therefore, understanding the details regarding WRKY TFs is very important. In this study, large-scale genomic analyses of the WRKY TF gene family from 43 plant species were conducted. The results of our study revealed that WRKY TFs could be grouped and specifically classified as those belonging to the monocot or dicot plant lineage...
November 17, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27847509/the-class-ii-trehalose-6-phosphate-synthase-gene-pvtps9-modulates-trehalose-metabolism-in-phaseolus-vulgaris-nodules
#2
Aarón Barraza, Cecilia Contreras-Cubas, Georgina Estrada-Navarrete, José L Reyes, Marco A Juárez-Verdayes, Nelson Avonce, Carmen Quinto, Claudia Díaz-Camino, Federico Sanchez
Legumes form symbioses with rhizobia, producing nitrogen-fixing nodules on the roots of the plant host. The network of plant signaling pathways affecting carbon metabolism may determine the final number of nodules. The trehalose biosynthetic pathway regulates carbon metabolism and plays a fundamental role in plant growth and development, as well as in plant-microbe interactions. The expression of genes for trehalose synthesis during nodule development suggests that this metabolite may play a role in legume-rhizobia symbiosis...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27830009/glycosylation-patterns-and-pha-e-associated-glycoprotein-profiling-associated-with-early-hepatic-encephalopathy-in-chinese-hepatocellular-carcinoma-patients
#3
Tian-Hua Liu, Deng-He Liu, Cui-Ju Mo, Lu Sun, Xiao-Xia Liu, Wei Li, Shu Zhang, Yin-Kun Liu, Kun Guo
Hepatic encephalopathy (HE) as a severe neuropsychiatric complication is commonly present in the end stage of Hepatocellular Carcinoma (HCC). However, widely accepted biomarkers for diagnosing early HE are still absent. Here, we screened glycosylation patterns of serum proteins from Chinese HCC patients with or without early HE by lectin microarray. Then, phaseolus vulgaris erythroagglutinin (PHA-E) as a lectin binding with bisecting GlcNAc structure which was significantly decreased in sera from Chinese HCC patients with early HE, was chosen to perform lectin affinity chromatography, following by in-gel digestion, Mass Spectrometry (MS) analysis and bioinformatics analysis...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27829044/transcriptome-profiling-of-the-phaseolus-vulgaris-colletotrichum-lindemuthianum-pathosystem
#4
Bilal A Padder, Kelvin Kamfwa, Halima E Awale, James D Kelly
Bean (Phaseolus vulgaris) anthracnose caused by the hemi-biotrophic pathogen Colletotrichum lindemuthianum is a major factor limiting production worldwide. Although sources of resistance have been identified and characterized, the early molecular events in the host-pathogen interface have not been investigated. In the current study, we conducted a comprehensive transcriptome analysis using Illumina sequencing of two near isogenic lines (NILs) differing for the presence of the Co-1 gene on chromosome Pv01 during a time course following infection with race 73 of C...
2016: PloS One
https://www.readbyqxmd.com/read/27813597/selection-of-common-bean-genotypes-for-the-cerrado-pantanal-ecotone-via-mixed-models-and-multivariate-analysis
#5
A M Corrêa, M I S Pereira, H K A de Abreu, T Sharon, C L P de Melo, M A Ito, P E Teodoro, L L Bhering
The common bean, Phaseolus vulgaris, is predominantly grown on small farms and lacks accurate genotype recommendations for specific micro-regions in Brazil. This contributes to a low national average yield. The aim of this study was to use the methods of the harmonic mean of the relative performance of genetic values (HMRPGV) and the centroid, for selecting common bean genotypes with high yield, adaptability, and stability for the Cerrado/Pantanal ecotone region in Brazil. We evaluated 11 common bean genotypes in three trials carried out in the dry season in Aquidauana in 2013, 2014, and 2015...
October 17, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27812443/environmental-surveillance-of-commonly-grown-vegetables-for-investigating-potential-lead-and-chromium-contamination-intensification-in-bangladesh
#6
A M M Maruf Hossain, M Shahidul Islam, M Mustafa Mamun, H M Al-Jonaed, M Imran, M Hasibur Rahman, M Azizul Islam Kazi, Syed Fazle Elahi
With regard to previously reported Lead (Pb) and Chromium (Cr) contamination in egg, a semi-quantitative assessment of the general environment of Bangladesh is carried out through nation-wide sampling of commonly grown and consumed vegetables. Five vegetables, namely, White Potato (Solanum tuberosum), Green Cabbage (Brassica oleracea capitata var. alba L.), Red Spinach (Amaranthus dubius), White Radish (Raphanus sativus var. longipinnatus), and Green Bean (Phaseolus vulgaris) were selected for sampling from all 64 Districts of the country as per their availability during the sampling season...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27811087/genome-sequence-of-paraburkholderia-nodosa-strain-cnpso-1341-a-n2-fixing-symbiont-of-the-promiscuous-legume-phaseolus-vulgaris
#7
Rebeca Fuzinatto Dall'Agnol, Maira Rejane Costa, Renan Augusto Ribeiro, Jakeline Renata Marçon Delamuta, Ligia Maria Oliveira Chueire, Mariangela Hungria
Paraburkholderia nodosa CNPSo 1341 is a N2-fixing symbiont of Phaseolus vulgaris isolated from an undisturbed soil of the Brazilian Cerrado. Its draft genome contains 8,614,032 bp and 8,068 coding sequences (CDSs). Nodulation and N2-fixation genes were clustered in the genome that also contains several genes of secretion systems and quorum sensing.
November 3, 2016: Genome Announcements
https://www.readbyqxmd.com/read/27807616/identification-of-bean-hydroxycinnamoyl-coa-tetrahydroxyhexanedioate-hydroxycinnamoyl-transferase-hhht-use-of-transgenic-alfalfa-to-determine-acceptor-substrate-specificity
#8
Michael L Sullivan
Transgenic alfalfa ( Medicago sativa L.) provides a useful reverse genetics platform to elucidate acceptor substrate specificity for uncharacterized BAHD family hydroxycinnamoyl-CoA hydroxycinnamoyl transferases. Tissues of many plant species accumulate hydroxycinnamoyl derivatives, often esters, thought to serve in protection against biotic and abiotic stresses. In many cases, these specialized metabolites are produced by BAHD family hydroxycinnamoyl-CoA hydroxycinnamoyl transferases (HCTs). Bean (Phaseolus vulgaris) leaves contain both hydroxycinnamoyl-malate esters and an HCT activity capable of making them...
November 2, 2016: Planta
https://www.readbyqxmd.com/read/27783982/the-response-of-aminopeptidases-of-phaseolus-vulgaris-to-drought-depends-on-the-developmental-stage-of-the-leaves
#9
Maruška Budič, Blaž Cigić, Maja Šoštarič, Jerica Sabotič, Vladimir Meglič, Janko Kos, Marjetka Kidrič
Aminopeptidases, together with other proteases, execute and regulate the total and specifically limited protein breakdown involved in plant physiology, raising the possibility of their involvement in response to drought. We have identified, in leaves of Phaseolus vulgaris L., five aminopeptidases (E.C.3.4.11) whose levels of activity changed when three week old plants were subjected to drought. First, second and third trifoliate leaves were investigated separately. The aminopeptidases were first identified then isolated using ion exchange chromatography of leaf extracts...
October 13, 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/27756730/effects-of-plant-intraspecific-diversity-across-three-trophic-levels-underlying-mechanisms-and-plant-traits
#10
Luis Abdala-Roberts, Johnattan Hernández-Cumplido, Luis Chel-Guerrero, David Betancur-Ancona, Betty Benrey, Xoaquín Moreira
PREMISE OF STUDY: Although there is increasing recognition of the effects of plant intraspecific diversity on consumers, the mechanisms by which such effects cascade-up to higher trophic levels remain elusive. METHODS: We evaluated the effects of plant (lima bean, Phaseolus lunatus) intraspecific diversity on a suite of insect herbivores (leaf-chewers, aphids, and seed-eating beetles) and their third trophic-level associates (parasitoids and aphid-tending ants)...
October 18, 2016: American Journal of Botany
https://www.readbyqxmd.com/read/27754657/demonstrating-a-nutritional-advantage-to-the-fast-cooking-dry-bean-phaseolus-vulgaris-l
#11
Jason A Wiesinger, Karen A Cichy, Raymond P Glahn, Michael A Grusak, Mark A Brick, Henry J Thompson, Elad Tako
Dry beans (Phaseolus vulgaris L.) are a nutrient dense food rich in protein and micronutrients. Despite their nutritional benefits, long cooking times limit the consumption of dry beans worldwide, especially in nations where fuelwood for cooking is often expensive or scarce. This study evaluated the nutritive value of 12 dry edible bean lines that vary for cooking time (20 to 89 min) from four market classes (yellow, cranberry, light red kidney, and red mottled) of economic importance in bean consuming regions of Africa and the Americas...
October 18, 2016: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/27754445/l-asparaginase-isolated-from-phaseolus-vulgaris-seeds-exhibited-potent-anti-acute-lymphoblastic-leukemia-effects-in-vitro-and-low-immunogenic-properties-in-vivo
#12
Saleh A Mohamed, Mohamed F Elshal, Taha A Kumosani, Alia M Aldahlawi, Tasneem A Basbrain, Fauziah A Alshehri, Hani Choudhry
Escherichia coli-derived L-asparaginases have been used in the treatment of acute lymphoblastic leukemia (ALL), however, clinical hypersensitivity reactions and silent inactivation due to antibodies against E. coli-asparaginase, lead to inactivation of these preparations in most cases.Therefore, this study was aimed to investigate the cytotoxicity and antitumor effects ofa novel L-asparaginaseenzyme, isolated from Phaseolus vulgaris seeds (P-Asp) on the ALL cell line (Jurkat). The immunogenicity of the enzyme was also evaluated in-vivo and results were compared to commercially available enzymes of microbial sources...
October 14, 2016: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/27734513/transformation-of-plastids-in-soil-shaded-lowermost-hypocotyl-segments-of-bean-phaseolus-vulgaris-during-a-60-day-cultivation-period
#13
Andrea Kakuszi, Katalin Solymosi, Béla Böddi
The maintenance but substantial transformation of plastids was found in lowermost hypocotyl segments of soil-grown bean plants (Phaseolus vulgaris cv. Magnum) during a 60-day cultivation period. Although the plants were grown under natural light-dark cycles, this hypocotyl segment was under full coverage of the soil in 5-7 cm depth, thus it was never exposed to light. The 4-day old plants were fully etiolated: amyloplasts, occasionally prolamellar bodies, protochlorophyllide (Pchlide) and protochlorophyll (Pchl) were found in the hypocotyls of these young seedlings...
October 13, 2016: Physiologia Plantarum
https://www.readbyqxmd.com/read/27721469/genomics-assisted-characterization-of-a-breeding-collection-of-apios-americana-an-edible-tuberous-legume
#14
Vikas Belamkar, Andrew D Farmer, Nathan T Weeks, Scott R Kalberer, William J Blackmon, Steven B Cannon
For species with potential as new crops, rapid improvement may be facilitated by new genomic methods. Apios (Apios americana Medik.), once a staple food source of Native American Indians, produces protein-rich tubers, tolerates a wide range of soils, and symbiotically fixes nitrogen. We report the first high-quality de novo transcriptome assembly, an expression atlas, and a set of 58,154 SNP and 39,609 gene expression markers (GEMs) for characterization of a breeding collection. Both SNPs and GEMs identify six genotypic clusters in the collection...
October 10, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27714495/molecular-and-biological-properties-of-an-endornavirus-infecting-winged-bean-psophocarpus-tetragonolobus
#15
Ryo Okada, Eri Kiyota, Hiromitsu Moriyama, Toshiyuki Fukuhara, Rodrigo A Valverde
A double-stranded RNA (dsRNA) of approximately 15 kbp was isolated from asymptomatic winged bean (Psophocarpus tetragonolobus) plants. The size of the dsRNA, together with results of RT-PCR testing, suggested that it was the replicative form of a plant endornavirus. Cloning, sequencing, and sequence analyses confirmed the endornavirus nature of the dsRNA. Conserved motifs typical for endornaviruses were identified and their amino acid sequences compared with those of selected endornaviruses. Phylogenetic analyses revealed a close relationship with Bell pepper endornavirus, Phaseolus vulgaris endornavirus 2, and Hot pepper endornavirus...
October 6, 2016: Virus Genes
https://www.readbyqxmd.com/read/27709374/exploring-drought-stress-regulated-genes-in-senna-cassia-angustifolia-vahl-a-transcriptomic-approach
#16
Rucha Harishbhai Mehta, Manivel Ponnuchamy, Jitendra Kumar, Nagaraja Reddy Rama Reddy
De novo assembly of reads produced by next-generation sequencing (NGS) technologies offers a rapid approach to obtain expressed gene sequences for non-model organisms. Senna (Cassia angustifolia Vahl.) is a drought-tolerant annual undershrub of Caesalpiniaceae, a subfamily of Fabaceae. There are insufficient transcriptomic and genomic data in public databases for understanding the molecular mechanism underlying the drought tolerance of senna. Therefore, the main purpose of this study was to know the transcriptome profile of senna, with special reference to drought stress...
October 5, 2016: Functional & Integrative Genomics
https://www.readbyqxmd.com/read/27706653/diallel-analysis-to-choose-parents-for-black-bean-phaseolus-vulgaris-l-breeding
#17
L M Moura, P C S Carneiro, N M Vale, L D Barili, L C Silva, J E S Carneiro, C D Cruz
In this study, conducted in two different seasons, we aimed to choose parents to obtain promising segregating populations for the extraction of black bean (Phaseolus vulgaris L.) lines that are superior in terms of disease resistance, plant architecture, and grain yield. Twelve parents were arranged in two groups to compose a partial diallel in a 5 x 7 scheme. Group 1 was composed of parents with black grains and erect plant architecture, while group 2 was composed of parents that had carioca grains and were resistant to the main fungal diseases that occur in the common bean...
August 29, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27706575/expression-and-validation-of-pvpgip-genes-for-resistance-to-white-mold-sclerotinia-sclerotiorum-in-common-beans-phaseolus-vulgaris-l
#18
R C C Vasconcellos, T F C Lima, C N Fernandes-Brum, A Chalfun-Junior, J B Santos
The interaction between polygalacturonase-inhibiting proteins (PGIPs), produced by plants, and endopolygalacturonases (PGs), produced by fungi, limits the destructive potential of PGs and can trigger plant defense responses. This study aimed to i) investigate variation in the expression of different common bean (Phaseolus vulgaris L.) genotypes and its relationship with resistance to white mold (Sclerotinia sclerotiorum); ii) determine the expression levels of PvPGIP genes at different time points after inoculation with white mold; and iii) investigate differences in PvPGIP gene expression between two white mold isolates with different levels of aggressiveness...
August 19, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27702841/regulation-of-stomatal-defense-by-air-relative-humidity
#19
Shweta Panchal, Reejana Chitrakar, Blaine K Thompson, Nisita Obulareddy, Debanjana Roy, W Sealy Hambright, Maeli Melotto
It has long been observed that environmental conditions play crucial roles in modulating immunity and disease in plants and animals. For instance, many bacterial plant disease outbreaks occur after periods of high humidity and rain. A critical step in bacterial infection is entry into the plant interior through wounds and natural openings, such as stomata, which are adjustable microscopic pores in the epidermal tissue. Several studies have shown that stomatal closure is an integral part of the plant immune response to reduce pathogen invasion...
November 2016: Plant Physiology
https://www.readbyqxmd.com/read/27700622/natural-postharvest-aflatoxin-occurrence-in-food-legumes-in-the-smallholder-farming-sector-of-zimbabwe
#20
David Tinayeshe Maringe, Cathrine Chidewe, Mudadi Albert Benhura, Brighton Marimanzi Mvumi, Tatenda Clive Murashiki, Mavis Precious Dembedza, Lucia Siziba, Loveness Kuziwa Nyanga
Aflatoxins, mainly produced by Aspergillus flavus and Aspergillus parasiticus, are highly toxic and may lead to health problems such as liver cancer. Exposure to aflatoxins may result from ingestion of contaminated foods. Levels of AFB1, AFB2, AFG1 and AFG2 in samples of groundnuts (Arachis hypogaea), beans (Phaseolus vulgaris), cowpeas (Vigna unguiculata) and bambara nuts (Vigna subterranean) grown by smallholder farmers in Shamva and Makoni districts, Zimbabwe, were determined at harvesting, using high performance liquid chromatography after immunoaffinity clean-up...
October 4, 2016: Food Additives & Contaminants. Part B, Surveillance
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