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Nicole Bandow, Stefan Gartiser, Outi Ilvonen, Ute Schoknecht
Construction products are in contact with water (e.g., rain, seepage water) during their service lifetime and may release potentially harmful compounds by leaching processes. Monitoring studies showed that compounds attributed to construction products are found in storm water and the receiving bodies of water and that the release of biocides in urban areas can be comparable to the input of pesticides from agricultural uses. Therefore, a prospective risk assessment of such products is necessary. Laboratory leaching tests have been developed by the Technical Committee CEN/TC 351 and are ready to use...
2018: Environmental Sciences Europe
Rosangela Tozzoli, Antonella Maugliani, Valeria Michelacci, Fabio Minelli, Alfredo Caprioli, Stefano Morabito
In 2006, the European Committee for standardisation (CEN)/Technical Committee 275 - Food analysis - Horizontal methods/Working Group 6 - Microbiology of the food chain (TC275/WG6), launched the project of validating the method ISO 16654:2001 for the detection of Escherichia coli O157 in foodstuff by the evaluation of its performance, in terms of sensitivity and specificity, through collaborative studies. Previously, a validation study had been conducted to assess the performance of the Method No 164 developed by the Nordic Committee for Food Analysis (NMKL), which aims at detecting E...
May 8, 2018: International Journal of Food Microbiology
Patricia Rollier, Bertrand Lombard, Laurent Guillier, Danièle François, Karol Romero, Sylvie Pierru, Laurence Bouhier, Nathalie Gnanou Besse
The reference method for the detection and enumeration of L. monocytogenes in food (Standards EN ISO 11290-1&2) have been validated by inter-laboratory studies in the frame of the Mandate M381 from European Commission to CEN. In this paper, the inter-laboratory studies led in 2013 on 5 matrices (cold-smoked salmon, milk powdered infant food formula, vegetables, environment, and cheese) to validate Standard EN ISO 11290-2 are reported. According to the results obtained, the method of the revised Standard EN ISO 11290-2 can be considered as a good method for the enumeration of L...
May 1, 2018: International Journal of Food Microbiology
Simona Giunta
Human centromeres are composed of large tandem arrays of repetitive alpha satellite DNA, which are often sites of aberrant rearrangement in cancers (Mitelman et al ., 1997; Padilla-Nash et al ., 2001). To date, annotation of the human centromere repetitive sequences remains incomplete, greatly hindering in-depth functional studies of these regions essential for chromosome segregation. In order to monitor sister chromatid exchange happening at the centromere (C-SCE) due to recombination and mutagenic events, I have applied the Chromosome-Orientation Fluorescence in situ Hybridization (CO-FISH) technique to centromeres (Cen-CO-FISH) in human cells...
April 5, 2018: Bio-protocol
Logesh Mathivathanan, Guang Yang, Fenfei Leng, Raphael G Raptis
Crystal structure determination of doxorubicin nitrate, (DoxH)NO3 , systematic name (7 S ,9 S )-7-{[(2 R ,4 S ,5 S ,6 S )-4-azaniumyl-5-hy-droxy-6-methyl-oxan-2-yl]-oxy}-6,9,11-trihy-droxy-9-(2-hy-droxy-acet-yl)-4-meth-oxy-8,10-di-hydro-7 H -tetra-cen-5,12-dione nitrate, shows two formula units present in the asymmetric unit. In the crystal lattice, hydrogen-bonded pairs of (DoxH+ ) cations and segregation of the aglycone and sugar moieties are observed. Inspection of mol-ecular overlays reveals that the conformation of (DoxH)NO3 resembles that of DNA-inter-calated, but not of protein-docked (DoxH)+ ...
March 1, 2018: Acta Crystallographica. Section E, Crystallographic Communications
Xiaogang Wu, Ningning Wang, Haipeng Cen, Zhaowei Wang, Weilun Yu, Kuijun Chen, Yanan Xue, Yanqin Wang, Yuan Guo, Weiyi Chen
There are two main types of fluid in bone tissue: blood and interstitial fluid. The metabolism of cells mainly relies on the microenvironment of the interstitial fluid. Researches of osteonal fluid seepage behavior based on the microstructure of bone tissue have become a hot point. The aim of the present research work is to assess the effect of blood pressure oscillation on the osteonal interstitial fluid seepage behavior. We established finite element osteon models for a hollow and that considering blood pressure oscillation, respectively, with COMSOL Multiphysics software in order to compare their fluid flow behavior under the axial loading...
August 1, 2017: Sheng Wu Yi Xue Gong Cheng Xue za Zhi, Journal of Biomedical Engineering, Shengwu Yixue Gongchengxue Zazhi
Minggang Cai, Haixia He, Mengyang Liu, Siwei Li, Guowen Tang, Weimin Wang, Peng Huang, Ge Wei, Yan Lin, Bin Chen, Jiahui Hu, Zhengnan Cen
Large quantities of microplastics with small particle sizes were found in the South China Sea (SCS). The abundances of microplastics in seawater were 0.045±0.093and 2569±1770particles/m3 according to the bongo net and pumping sampling methods, respectively. Smaller-size fractions (size<0.3mm) contributed 92% of the number of microplastics to the total load. Continental slope is the largest reservoir of microplastics with an inventory of 295tons. 21 polymer types were found in the samples using the micro Fourier Transform Infrared Spectroscopy (FTIR), among which alkyds (22...
August 15, 2018: Science of the Total Environment
Hongchun Li, Wei Xu, Linhong Jiang, Hui Gu, Menglu Li, Jiamei Zhang, Wei Guo, Pengchi Deng, Hailei Long, Qian Bu, Jingwei Tian, Yinglan Zhao, Xiaobo Cen
Alcohol abuse and its related diseases are the major risk factors for human health. Although the mechanism of alcohol-related disorders has been widely investigated, serum metabolites associated with long-term alcohol intake have not been well explored. In this study, we aimed to investigate the profiles of serum metabolites and lipid species of rats chronically exposed to alcohol, which may be involved in the pathogenesis of alcohol-associated disease. An 1 H NMR-based metabolomics and Q-TOF/MS-based lipidomics approach were applied to investigate the profile of serum metabolites and lipid species of rats administrated daily with alcohol (12% vol/vol, 10 ml/kg per day, i...
May 11, 2018: Toxicology Letters
P H In 't Veld, L F J van der Laak, M van Zon, E G Biesta-Peters
A method for the quantification of the Bacillus cereus emetic toxin (cereulide) was developed and validated. The method principle is based on LC-MS as this is the most sensitive and specific method for cereulide. Therefore the study design is different from the microbiological methods validated under this mandate. As the method had to be developed a two stage validation study approach was used. The first stage (pre-study) focussed on the method applicability and the experience of the laboratories with the method...
April 12, 2018: International Journal of Food Microbiology
Madhusudhan Srinivasan, Johanna C Scheinost, Naomi J Petela, Thomas G Gligoris, Maria Wissler, Sugako Ogushi, James E Collier, Menelaos Voulgaris, Alexander Kurze, Kok-Lung Chan, Bin Hu, Vincenzo Costanzo, Kim A Nasmyth
As predicted by the notion that sister chromatid cohesion is mediated by entrapment of sister DNAs inside cohesin rings, there is perfect correlation between co-entrapment of circular minichromosomes and sister chromatid cohesion. In most cells where cohesin loads without conferring cohesion, it does so by entrapment of individual DNAs. However, cohesin with a hinge domain whose positively charged lumen is neutralized loads and moves along chromatin despite failing to entrap DNAs. Thus, cohesin engages chromatin in non-topological, as well as topological, manners...
April 30, 2018: Cell
Jueying Kong, Changman Du, Linhong Jiang, Wei Jiang, Pengchi Deng, Xue Shao, Baolai Zhang, Yan Li, Ruiming Zhu, Qian Zhao, Dengqi Fu, Hui Gu, Li Luo, Hailei Long, Yinglan Zhao, Xiaobo Cen
Nicotinamide phosphoribosyltransferase (NAMPT), a rate-limiting enzyme in nicotinamide adenine dinucleotide (NAD) biosynthesis in mammals, converts nicotinamide into nicotinamide mononucleotide (NMN). NMN is subsequently converted to NAD, a component that is critical for cell energy metabolism and survival. Sirtuin 1 (SIRT1), an NAD-dependent histone deacetylase, plays an important role in mediating memory and synaptic plasticity. Here, we found that NAMPT was significantly upregulated in the ventral tegmental area (VTA) of cocaine-conditioned mice...
May 10, 2018: Experimental Neurology
Ying Wang, Tingting Shen, Peiliang Fang, Junbo Zhou, Kenan Lou, Zemin Cen, Hai Qian, Jianqing Zhou, Ningsheng Liu, Jiangfang Lian
Background: LQT2 was caused by HERG mutation. L539fs/47 encodes a truncated protein,and its mechanisms in HERG mutation is unknown. Method: HERG mutation plasmids were overexpressed in HEK293T cells respectively, followed by analyzing lysates with western-blot. Transfected HEK293T cells were treated with or without ALLN and Propranolol 24hrs or 48hrs. HERG-WT, HERG-A561V, WT/A561V, HERG-L539fs/47, WT/L539fs/47 and calnexin/calreticulin protein expressing and their interactions were detected by western blot and immunoprecipitation...
May 11, 2018: Bioscience Reports
Hui-Sheng Yang, Yan-Ming Xie, Cen Chen, Yan Zhuang, Yin Zhang
The present study was aimed to analyze the association rules of Fufang Kushen injection in combination with other modern medications in treating lung cancer based on the electrical medical records in real world clinical situations extracted from hospital information system in Institute of Basic Medical Sciences, China Academy of Chinese Medical Sciences, and provide reference for improving rationality of clinical drug use and exploring the prescription rules as well as subsequent in-depth analysis. The electrical medical records of the hospitalized patients using Fufang Kushen injection for lung cancer were extracted to analyze the frequency distribution characteristics in combined application with western medicine, and Apriori algorithm was used to analyze the specific association rules in combined drug use...
April 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Qianjun Jia, Jianzheng Cen, Jinglei Li, Jian Zhuang, Hui Liu, Qun Zhang, Xiaoqing Liu, Meiping Huang, Changhong Liang
The original version of this article, originally published online ahead of printing on January 05, 2018, unfortunately contained mistakes.
May 7, 2018: European Radiology
Tengfei Hao, Qizhuang Cen, Yitang Dai, Jian Tang, Wei Li, Jianping Yao, Ninghua Zhu, Ming Li
An optoelectronic oscillator (OEO) is a microwave photonic system with a positive feedback loop used to create microwave oscillation with ultra-low phase noise thanks to the employment of a high-quality-factor energy storage element, such as a fiber delay line. For many applications, a frequency-tunable microwave signal or waveform, such as a linearly chirped microwave waveform (LCMW), is also needed. Due to the long characteristic time constant required for building up stable oscillation at an oscillation mode, it is impossible to generate an LCMW with a large chirp rate using a conventional frequency-tunable OEO...
May 9, 2018: Nature Communications
Kangwei Li, Linghong Chen, Stephen J White, Hai Yu, Xuecheng Wu, Xiang Gao, Merched Azzi, Kefa Cen
Ammonia (NH3 ) is a major contributor to secondary aerosol in the atmosphere and can alter the kinetics of their formation. However, systematic studies related to the role of NH3 in aerosol nucleation processes and further particle size growth under complex scenarios are lacking. In this study, we conducted 16 experiments in the CSIRO smog chamber under dry conditions using aromatic hydrocarbons (toluene, o-/m-/p-xylene) and different concentrations of NH3 . The presence of NH3 did not change the gas-phase chemistry or nucleation onset time, but slowed the nucleation rate (5%-94%) once it began...
April 1, 2018: Science of the Total Environment
Lei Cui, Xinxin Lu, Yuelei Dong, Jingyi Cen, Rongbo Cao, Lin Pan, Songhui Lu, Linjian Ou
The picoplanktonic pelagophyte Aureococcus anophagefferens could trigger harmful algal blooms (HABs) to discolor water in brown, known as brown tide. Since 2009, large-scale brown tides, caused by A. anophagefferens, had been occurred in early summer for three consecutive years in the coastal waters of Qinhuangdao, China and resulted considerable deleterious effects on the scallop mariculture industry. The causes for the occurrence of brown tides were not fully understood. Therefore, we conducted a one-year survey from June 2013 to May 2014 to study the seasonal succession of the phytoplankton community, including A...
May 3, 2018: Ecotoxicology and Environmental Safety
Peng Xu, Changli Cen, Nannan Chen, Huan Lin, Qing Wang, Nanfang Xu, Jingen Tang, Zhaogang Teng
In this study, we have prepared silver nanoparticles deposited cellulose microfibers (Ag-CMFs) with excellent catalytic property. The cellulose microfibers with a mean diameter of approximately 270 nm are regenerated by deacetylation of cellulose acetate microfibers. Silver nanoparticles are deposited onto the surface of the cellulose microfibers by a simple wet reduction of silver precursor using glucose as reducing agent. The morphology, thermal stability and catalytic activities of the samples have been investigated by scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TG), and UV-vis spectrophotometer...
April 11, 2018: Journal of Colloid and Interface Science
Fengnian Wu, Jiaquan Huang, Meirong Xu, Eduardo G P Fox, G Andrew C Beattie, Paul Holford, Yijing Cen, Xiaoling Deng
BACKGROUND: Diaphorina citri is a vector of "Candidatus Liberibacter asiaticus" (CLas) associated with citrus Huanglongbing. In this study, the infection and titers of CLas in the psyllid, were monitored for life cycle stage, sex, host-plant CLas titer, host-plant genotype, and ambient temperature. RESULTS: Acquisition efficiency of CLas by D. citri was highest in nymphs reared at 25 °C on a host plant with high CLas titers but was independent of the host genotypes assessed and of vector sex...
May 3, 2018: Pest Management Science
Amparo de Benito, Nathalie Gnanou Besse, Isabelle Desforges, Barbara Gerten, Begoña Ruiz, David Tomás
The new version of the ISO standard method for detection of Cronobacter spp. (EN ISO 22964:2017) was validated in the frame of the European Commission Mandate M381 to CEN. Seventeen laboratories from nine countries participated in the interlaboratory studies to determine the performance characteristics of the method. The performance of the method was evaluated using matrices for which the presence of Cronobacter spp. is considered to be of serious concern, such as infant formula and its ingredients and representatives of categories cited in the EC Regulation 2073/2005 on microbiological criteria for foodstuffs for Cronobacter spp...
April 19, 2018: International Journal of Food Microbiology
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