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Stromal vascular fraction

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https://www.readbyqxmd.com/read/27913088/effect-of-suction-pressures-on-cell-yield-and-functionality-of-the-adipose-derived-stromal-vascular-fraction
#1
Yuan-Wen Chen, Jing-Ru Wang, Xuan Liao, Sheng-Hong Li, Li-Ling Xiao, Biao Cheng, Guang-Hui Xie, Jian-Xing Song, Hong-Wei Liu
OBJECTIVE: The present study was performed to explore the effect of suction pressures on the cell yield of adipose-derived stromal vascular fraction (SVF) and the functionality of adipose-derived stem cells (ADSCs), situated in the SVF, and to develop optimal parameters of harvesting SVF for clinical use. METHODS: Adipose tissue was harvested from the lower abdomen of 10 patients by suction-assisted lipoplasty. Suction pressure was either -30 ± 5 kPa or -55 ± 5 kPa...
November 11, 2016: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
https://www.readbyqxmd.com/read/27902740/extensive-characterization-and-comparison-of-endothelial-cells-derived-from-dermis-and-adipose-tissue-potential-use-in-tissue-engineering
#2
Hanneke N Monsuur, Ester M Weijers, Frank B Niessen, Amit Gefen, Pieter Koolwijk, Susan Gibbs, Lenie J van den Broek
Tissue-engineered constructs need to become quickly vascularized in order to ensure graft take. One way of achieving this is to incorporate endothelial cells (EC) into the construct. The adipose tissue stromal vascular fraction (adipose-SVF) might provide an alternative source for endothelial cells as adipose tissue can easily be obtained by liposuction. Since adipose-EC are now gaining more interest in tissue engineering, we aimed to extensively characterize endothelial cells from adipose tissue (adipose-EC) and compare them with endothelial cells from dermis (dermal-EC)...
2016: PloS One
https://www.readbyqxmd.com/read/27886006/semi-automated-extraction-and-characterization-of-stromal-vascular-fraction-using-a-new-medical-device
#3
Alexander Hanke, Lukas Prantl, Carina Wenzel, Michael Nerlich, Gero Brockhoff, Markus Loibl, Sebastian Gehmert
INTRODUCTION: The stem cell rich Stromal Vascular Fraction (SVF) can be harvested by processing lipo-aspirate or fat tissue with an enzymatic digestion followed by centrifugation. To date neither a standardised extraction method for SVF nor a generally admitted protocol for cell application in patients exists. A novel commercially available semi-automated device for the extraction of SVF promises sterility, consistent results and usability in the clinical routine. The aim of this work was to compare the quantity and quality of the SVF between the new system and an established manual laboratory method...
November 23, 2016: Clinical Hemorheology and Microcirculation
https://www.readbyqxmd.com/read/27882150/effect-of-stromal-cell-derived-factor-1-on-myocardial-apoptosis-and-cardiac-function-recovery-in-rats-with-acute-myocardial-infarction
#4
Yuanyuan Liu, Songtao Gao, Zheng Wang, Yan Yang, Hong Huo, Xuefeng Tian
The aim of the study was to investigate the effect of stromal cell-derived factor-1 (SDF-1) on myocardial apoptosis and cardiac function recovery in rats with acute myocardial infarction (AMI) and the mechanism of the Toll-like receptor (TLR)-4/nuclear factor-κB (NF-κB) signaling pathway. A total of 64 healthy male F344 rats were randomly divided into the sham operation, model, SDF-1 intervention and SDF-1 antibody groups, with 16 rats in each group. The method of Olivette was used to establish the AMI model by ligation of the left anterior descending artery...
November 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/27853678/rejuvenation-of-facial-skin-and-improvement-in-the-dermal-architecture-by-transplantation-of-autologous-stromal-vascular-fraction-a-clinical-study
#5
Mohammad Amir Amirkhani, Alireza Shoae-Hassani, Masoud Soleimani, Somayeh Hejazi, Leila Ghalichi, Mohammad Ali Nilforoushzadeh
Introduction: The rejuvenation characteristics of fat tissue grafting has been established for many years. Recently it has been shown that stromal vascular fraction (SVF) of fat tissue contributes to its rejuvenation properties. As the SVF is a minimal processed cell population (based on FDA guidance), therefore it is a suitable cell therapy for skin rejuvenation. This clinical trial was aimed to evaluate the ultrastructural improvement of aging skin in the facial nasolabial region after transplantation of autologous SVF...
2016: BioImpacts: BI
https://www.readbyqxmd.com/read/27852267/culture-expansion-of-adipose-derived-stromal-cells-a-closed-automated-quantum-cell-expansion-system-compared-with-manual-flask-based-culture
#6
Mandana Haack-Sørensen, Bjarke Follin, Morten Juhl, Sonja K Brorsen, Rebekka H Søndergaard, Jens Kastrup, Annette Ekblond
BACKGROUND: Adipose derived stromal cells (ASCs) are a rich and convenient source of cells for clinical regenerative therapeutic approaches. However, applications of ASCs often require cell expansion to reach the needed dose. In this study, cultivation of ASCs from stromal vascular fraction (SVF) over two passages in the automated and functionally closed Quantum Cell Expansion System (Quantum system) is compared with traditional manual cultivation. METHODS: Stromal vascular fraction was isolated from abdominal fat, suspended in α-MEM supplemented with 10% Fetal Bovine Serum and seeded into either T75 flasks or a Quantum system that had been coated with cryoprecipitate...
November 16, 2016: Journal of Translational Medicine
https://www.readbyqxmd.com/read/27810224/the-utility-of-adipose-derived-stem-cells-and-stromal-vascular-fraction-for-oncologic-soft-tissue-reconstruction-is-it-safe-a-matter-for-debate
#7
Shaun S Tan, Weiliang Loh
Autologous free-fat grafting (AFG) has emerged as an attractive proposition for soft-tissue reconstruction of various contour defects because it obviates more complex reconstructive options and reduces operative times and donor-site morbidity. Nonetheless, a common complication of this procedure is the resorption of the engrafted fat. Cell-assisted lipotransfer (CAL) is now a well-regarded technique where adipose-derived stem cell (ASC)-rich stromal vascular fraction is admixed with lipoaspirate, increasing the volumetric outcome of fat grafts in light of its potent angiogenic and adipogenic properties...
November 1, 2016: Surgeon: Journal of the Royal Colleges of Surgeons of Edinburgh and Ireland
https://www.readbyqxmd.com/read/27777379/impaired-autophagy-activity-is-linked-to-elevated-er-stress-and-inflammation-in-aging-adipose-tissue
#8
Amiya Kumar Ghosh, Theresa Mau, Martin O'Brien, Sanjay Garg, Raymond Yung
Adipose tissue dysfunction in aging is associated with inflammation, metabolic syndrome and other diseases. We propose that impaired protein homeostasis due to compromised lysosomal degradation (micro-autophagy) might promote aberrant ER stress response and inflammation in aging adipose tissue. Using C57BL/6 mouse model, we demonstrate that adipose tissue-derived stromal vascular fraction (SVF) cells from old (18-20 months) mice have reduced expression of autophagy markers as compared to the younger (4-6 months) cohort...
October 24, 2016: Aging
https://www.readbyqxmd.com/read/27761084/role-of-adipose-derived-stem-cells-in-fat-grafting-and-reconstructive-surgery
#9
Shaun S Tan, Zhi Yang Ng, Weiqing Zhan, Warren Rozen
Autologous fat grafting is commonly utilised to reconstruct soft tissue defects caused by ageing, trauma, chronic wounds and cancer resection. The benefits of fat grafting are minimal donor site morbidity and ease of availability through liposuction or lipectomy. Nonetheless, survival and longevity of fat grafts remain poor post-engraftment. Various methods to enhance fat graft survival are currently under investigation and its stem cell constituents are of particular interest. Cell-assisted lipotransfer refers to the addition of adipose-derived stem cell (ASC) rich component of stromal vascular fraction to lipoaspirate, the results of which have proven promising...
July 2016: Journal of Cutaneous and Aesthetic Surgery
https://www.readbyqxmd.com/read/27757364/microcirculatory-response-in-vivo-on-local-intraarterial-infusion-of-autogenic-adipose-derived-stem-cells-or-stromal-vascular-fraction
#10
Wei Z Wang
: Both adipose-derived stem cells (ASCs) and stromal vascular fraction (SVF) have been demonstrated to have regenerative properties with therapeutic potential for numerous diseases through local or topical applications. However, it is unclear whether ASC or SVF can be delivered systemically through an intra-arterial infusion. The purpose of this study was to examine the microcirculatory response in vivo on local intraarterial infusion of autogenic ASCs or SVF in a vascular pedicle isolated rat cremaster microcirculation model...
September 2016: Plastic and Reconstructive Surgery. Global Open
https://www.readbyqxmd.com/read/27757339/shift-toward-mechanical-isolation-of-adipose-derived-stromal-vascular-fraction-review-of-upcoming-techniques
#11
Alexandra Condé-Green, Vasanth S Kotamarti, Lauren S Sherman, Jonathan D Keith, Edward S Lee, Mark S Granick, Pranela Rameshwar
: Standard isolation of adipose stromal vascular fraction (SVF) requires the use of collagenase and is considered more than "minimally manipulated" by current good manufacturing practice requirements. Alternatively, nonenzymatic isolation methods have surfaced using physical forces to separate cells from the adipose matrix. The purpose of this study was to review the literature on the use of mechanical isolation protocols and compare the results. The implication for use as a standard procedure in practice is discussed...
September 2016: Plastic and Reconstructive Surgery. Global Open
https://www.readbyqxmd.com/read/27755302/mechanically-isolated-stromal-vascular-fraction-provides-a-valid-and-useful-collagenase-free-alternative-technique-a-comparative-study
#12
Cédric Ménard
No abstract text is available yet for this article.
October 10, 2016: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/27755299/-mechanical-isolation-of-stromal-vascular-fraction-a-new-advance-in-stem-cells-therapy
#13
Benoit Chaput, Nicolas Bertheuil, Jean-Louis Grolleau, Ignacio Garrido, Jerome Laloze, Audrey Varin
No abstract text is available yet for this article.
October 10, 2016: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/27748745/meteorin-like-metrnl-decreases-during-adipogenesis-and-inhibits-adipocyte-differentiation-leading-to-adipocyte-hypertrophy-in-humans
#14
D Löffler, K Landgraf, D Rockstroh, J T Schwartze, H Dunzendorfer, W Kiess, A Körner
BACKGROUND: Meteorin-like (METRNL) is a recently described circulating protein shown to be highly expressed in white adipose tissue and to beneficially affect energy metabolism in mice. OBJECTIVE: We systematically evaluated the role of METRNL for human adipogenesis and its association with obesity, browning and hyperinsulinemia in children. In addition, we assessed the functional relevance of METRNL for human adipogenesis. RESULTS: METRNL expression decreased during human adipocyte differentiation in vitro...
October 17, 2016: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/27738018/the-distribution-and-adipogenic-potential-of-perivascular-adipose-tissue-adipocyte-progenitors-is-dependent-on-sexual-dimorphism-and-vessel-location
#15
G Andres Contreras, Kyan Thelen, Nadia Ayala-Lopez, Stephanie W Watts
There are sex associated differences in the risk for cardiovascular comorbidities in obesity and metabolic syndrome. A common clinical finding in these diseases is the expansion of perivascular adipose tissues (PVAT) which is associated with alterations in their role as regulators of vessel function. PVAT hyperplasia and hypertrophy are dependent on the biology of populations of adipocyte progenitor cells (APC). It is currently unclear if PVAT enlargement diverges between males and females and the mechanisms linking APC biology with sexual dimorphism remain poorly understood...
October 2016: Physiological Reports
https://www.readbyqxmd.com/read/27737342/adipose-tissue-stromal-vascular-fraction-in-the-treatment-of-full-thickness-burns-in-rats
#16
Alexandre Lamaro Cardoso, Maria Márcia Bachion, Júlia de Miranda Morais, Marcelo Silva Fantinati, Vera Lúcia Lima de Almeida, Ruy Souza Lino
PURPOSE: To analyze the healing effects of stromal vascular fraction (SVF) application compared to wound dressing with 2% silver sulfadiazine in full thickness burn wounds in rats. METHODS: Animals were divided into two groups: 2% silver sulfadiazine group and SVF group. Both groups received occlusive bandages while the first one was treated with 2% silver sulfadiazine and the latter was treated with injections of SVF prepared from adipose tissue extracted from an animal donor...
September 2016: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/27733784/evaluation-of-adipose-derived-stromal-vascular-fraction-from-the-lateral-tailhead-inguinal-region-and-mesentery-of-horses
#17
Garrett L Metcalf, Scott R McClure, Jesse M Hostetter, Rudy F Martinez, Chong Wang
Use of mesenchymal stem cells (MSCs) found in the stromal vascular fraction (SVF) of equine adipose tissue has promising applications for regenerative therapies. The most commonly used source of equine adipose tissue is the subcutaneous tailhead. The objective of this study was to compare 3 adipose depot sites in horses and determine the viability and cellular yield, capillary density, gene expression for selected markers, and colony-forming unit fibroblasts (CFU-Fs) in adipose tissue taken from these sites...
October 2016: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://www.readbyqxmd.com/read/27733015/adipose-stromal-vascular-fraction-mediated-improvements-at-late-stage-disease-in-a-murine-model-of-multiple-sclerosis
#18
Annie C Bowles, Amy L Strong, Rachel M Wise, Robert C Thomas, Brittany Y Gerstein, Maria F Dutreil, Ryan S Hunter, Jeffrey M Gimble, Bruce A Bunnell
Multiple sclerosis (MS) is a common neurodegenerative disease and remains an unmet clinical challenge. In MS, an autoimmune response leads to immune cell infiltration, inflammation, demyelination, and lesions in central nervous system (CNS) tissues resulting in tremors, fatigue, and progressive loss of motor function. These pathologic hallmarks are effectively reproduced in the murine experimental autoimmune encephalomyelitis (EAE) model. The stromal vascular fraction (SVF) of adipose tissue is composed of adipose-derived stromal/stem cells (ASC), adipocytes, and various leukocytes...
October 12, 2016: Stem Cells
https://www.readbyqxmd.com/read/27731522/the-novel-treatment-of-svf-enriched-fat-grafting-for-painful-end-neuromas-of-superficial-radial-nerve
#19
Maurizio Calcagni, Simon Zimmermann, Mario F Scaglioni, Thomas Giesen, Pietro Giovanoli, Richard M Fakin
INTRODUCTION: None of the existing treatments in the management of painful end-neuromas of the superficial branch of the radial nerve (SBRN) has been proven superior due to high levels of pain relapse. Fat grafts enriched with the stromal vascular fraction (SVF) could act as a mechanic barrier with biological effects decreasing the resorption rate and boosting the graft's regenerative potential. This study describes the novel surgical treatment technique of SVF-enriched fat grafting. PATIENTS AND METHODS: In this clinical study, five consecutive patients treated for painful end-neuromas of the SBRN between 2012 and 2013 were analyzed retrospectively...
October 12, 2016: Microsurgery
https://www.readbyqxmd.com/read/27729337/divergent-functions-of-endotrophin-on-different-cell-populations-in-adipose-tissue
#20
Yueshui Zhao, Xue Gu, Ningyan Zhang, Mikhail G Kolonin, Zhiqiang An, Kai Sun
Endotrophin is a cleavage product of collagen 6 (Col6) in adipose tissue (AT). Previously, we demonstrated that endotrophin serves as a costimulator to trigger fibrosis and inflammation within the unhealthy AT milieu. However, how endotrophin affects lipid storage and breakdown in AT and how different cell types in AT respond to endotrophin stimulation remain unknown. In the current study, by using a doxycycline (Dox)-inducible mouse model, we observed significant upregulation of adipogenic genes in the WAT of endotrophin Tg mice...
October 11, 2016: American Journal of Physiology. Endocrinology and Metabolism
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