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Stromal vascular fraction

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https://www.readbyqxmd.com/read/28231331/mesenchymal-stromal-cells-mscs-induce-ex-vivo-proliferation-and-erythroid-commitment-of-cord-blood-haematopoietic-stem-cells-cb-cd34-cells
#1
Simone Perucca, Andrea Di Palma, Pier Paolo Piccaluga, Claudia Gemelli, Elisa Zoratti, Giulio Bassi, Edoardo Giacopuzzi, Andrea Lojacono, Giuseppe Borsani, Enrico Tagliafico, Maria Teresa Scupoli, Simona Bernardi, Camilla Zanaglio, Federica Cattina, Valeria Cancelli, Michele Malagola, Mauro Krampera, Mirella Marini, Camillo Almici, Sergio Ferrari, Domenico Russo
A human bone marrow-derived mesenchymal stromal cell (MSCs) and cord blood-derived CD34+ stem cell co-culture system was set up in order to evaluate the proliferative and differentiative effects induced by MSCs on CD34+ stem cells, and the reciprocal influences on gene expression profiles. After 10 days of co-culture, non-adherent (SN-fraction) and adherent (AD-fraction) CD34+ stem cells were collected and analysed separately. In the presence of MSCs, a significant increase in CD34+ cell number was observed (fold increase = 14...
2017: PloS One
https://www.readbyqxmd.com/read/28230922/adipose-derived-aldehyde-dehydrogenase-expressing-cells-promote-dermal-regenerative-potential-with-collagen-glycosaminoglycan-scaffold
#2
Hajime Matsumine, Kazuyuki Numakura, Satoshi Tsunoda, Huan Wang, Rui Matsumine, Mihail Climov, Giorgio Giatsidis, Vikas P Sukhatme, Dennis P Orgill
Aldehyde dehydrogenase (ALDH) is an enzyme that plays an important role in retinoid metabolism and highly expressed in stem cells. This study isolated ALDH-expressing cells from subcutaneous adipose tissue and investigated their potential to enhance healing in a full-thickness skin wound in rats by co-implanting them with collagen-glycosaminoglycan (c-GAG) scaffolds. ALDH-positive cells were isolated by a fluorescence-activated cell sorting technique from Lewis rat's stromal-vascular-fraction (SVF) and transplanted with c-GAG scaffolds in a rat full-thickness skin wound model...
November 7, 2016: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/28229415/isolation-of-stromal-stem-cells-from-adipose-tissue
#3
Maria Prat, Francesca Oltolina, Silvia Antonini, Andrea Zamperone
Adipose tissue has been shown to be particularly advantageous as source of mesenchymal stem cells (MSCs), because of its easy accessibility, and the possibility of obtaining stem cells in high yields. MSCs are obtained from the so-called Stromal Vascular Fraction, (SVF), exploiting their property of adhering to plastic surfaces and can be further purified by positive or negative immunomagnetic selection with appropriately chosen antibodies. These cells (Stromal Stem Cells, SSCs) can then be directly analyzed, frozen in liquid nitrogen, or expanded for further applications, e...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28228728/perivascular-adipose-tissue-s-impact-on-norepinephrine-induced-contraction-of-mesenteric-resistance-arteries
#4
Nadia Ayala-Lopez, Janice M Thompson, Stephanie W Watts
Background: Perivascular adipose tissue (PVAT) can decrease vascular contraction to NE. We tested the hypothesis that metabolism and/or uptake of vasoactive amines by mesenteric PVAT (MPVAT) could affect NE-induced contraction of the mesenteric resistance arteries. Methods: Mesenteric resistance vessels (MRV) and MPVAT from male Sprague-Dawley rats were used. RT-PCR and Western blots were performed to detect amine metabolizing enzymes. The Amplex® Red Assay was used to quantify oxidase activity by detecting the oxidase reaction product H2O2 and the contribution of PVAT on the mesenteric arteries' contraction to NE was measured by myography...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28222464/regulation-of-dipeptidyl-peptidase-4-its-substrate-chemokines-and-their-receptors-in-adipose-tissue-of-ob-ob-mice
#5
Jihoon Shin, Atsunori Fukuhara, Toshiharu Onodera, Chieko Yokoyama, Michio Otsuki, Iichiro Shimomura
The physiological function of DPP-4 in proteolytic inactivation of incretins has been well established, however, there is limited information on the expression and the significance of DPP-4 in white adipose tissue with regard to obesity. The objective of the work was to reveal the expression and regulation of DPP-4 in adipocytes and compare the expression and activity of DPP-4 in white adipose tissue and several other organs such as the liver, muscle and kidney. We also investigated the gene expression levels of DPP-4 substrate chemokines, and their receptors in white adipose tissue...
February 21, 2017: Hormone and Metabolic Research, Hormon- und Stoffwechselforschung, Hormones et Métabolisme
https://www.readbyqxmd.com/read/28209631/hdpscs-fabricate-a-vascularised-woven-bone-tissue-a-new-tool-in-bone-tissue-engineering
#6
Francesca Paino, Marcella La Noce, Alessandra Giuliani, Alfredo De Rosa, Serena Mazzoni, Luigi Laino, Evzen Amler, Gianpaolo Papaccio, Vincenzo Desiderio, Virginia Tirino
Human dental pulp stem cells (hDPSCs) are mesenchymal stem cells successfully used in human bone tissue engineering. To better establish if these cells can lead to a bone tissue ready to be grafted, we checked DPSCs for their osteogenic and angiogenic differentiation capabilities with the specific aim to obtain a new tool for bone transplantation. Therefore, hDPSCs were specifically selected from the stromal-vascular dental pulp fraction, using appropriate markers and cultured. Growth curves, expression of bone-related markers, calcification, angiogenesis as well as in vivo transplantation assay were performed...
February 16, 2017: Clinical Science (1979-)
https://www.readbyqxmd.com/read/28209525/stromal-vascular-fraction-shows-robust-wound-healing-through-high-chemotactic-and-epithelialization-property
#7
Dong-Sic Chae, Seongho Han, Mina Son, Sung-Whan Kim
BACKGROUND: Although human stromal vascular fraction (SVF) has been regarded as an attractive stem cell source, its therapeutic mechanism in wound healing has not been fully elucidated. AIMS: In this study, we investigated the molecular characteristics and therapeutic property of SVF for wound healing. METHODS: Microarray data showed that SVF cells are enriched with a higher level of wound healing or epithelium development-related genes and micro RNA...
February 13, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28191379/functional-and-aesthetic-recovery-of-congenital-muscular-torticollis-with-intramuscular-stromal-vascular-fraction-enriched-fat-grafting
#8
Juan Monreal
Congenital muscular torticollis is a well-known pathological condition caused by the contracture and shortening of the sternocleidomastoid muscle. This condition is manifested by a neck postural deformity often accompanied by some kind of facial asymmetry. Once diagnosed, treatment by early physiotherapy is generally successful in a high percentage of patients if performed during the first year of life. Later, especially after the fourth year, conservative treatment is usually far less effective, and surgical techniques remain the only way to improve neck contour and function...
January 12, 2017: Curēus
https://www.readbyqxmd.com/read/28186685/concise-review-fat-and-furious-harnessing-the-full-potential-of-adipose-derived-stromal-vascular-fraction
#9
Jordan A Dykstra, Tiffany Facile, Ryan J Patrick, Kevin R Francis, Samuel Milanovich, Jill M Weimer, Daniel J Kota
Due to their capacity to self-renew, proliferate and generate multi-lineage cells, adult-derived stem cells offer great potential for use in regenerative therapies to stop and/or reverse degenerative diseases such as diabetes, heart failure, Alzheimer's disease and others. However, these subsets of cells can be isolated from different niches, each with differing potential for therapeutic applications. The stromal vascular fraction (SVF), a stem cell enriched and adipose-derived cell population, has garnered interest as a therapeutic in regenerative medicine due to its ability to secrete paracrine factors that accelerate endogenous repair, ease of accessibility and lack of identified major adverse effects...
January 6, 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28177263/isolation-of-adipose-derived-stromal-vascular-fraction-cells-using-a-novel-point-of-care-device-cell-characterization-and-review-of-the-literature
#10
James Christian Brown, Hulan Shang, Ying Li, Ning Yang, Nikita Patel, Adam J Katz
The GID SVF-1 and GID SVF-2 are closed-system, disposable, scalable cellular isolation devices designed for isolating the human adipose-derived stromal vascular fraction (hSVF) from lipoaspirates at the point-of care. The purpose of this study was to characterize the device performance with respect to cell yield and viability of the hSVF, as well as the hSVF purity and cellular composition. Our results demonstrate that adipose-derived SVF can be safely obtained using both devices and standardized methods, yielding cells that are free of bacterial contaminants as measured by endotoxin levels, gram stain and culture...
February 8, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28174981/op008-an-innovative-treatment-for-refractory-perianal-fistulas-in-crohn-s-disease-local-micro-reinjection-of-autologous-fat-and-adipose-derived-stromal-vascular-fraction
#11
M Serrero, C Philandrianos, C Visee, J Veran, P Orsoni, F Sabatier, J-C Grimaud
No abstract text is available yet for this article.
February 1, 2017: Journal of Crohn's & Colitis
https://www.readbyqxmd.com/read/28170179/comparative-clinical-observation-of-arthroscopic-microfracture-in-the-presence-and-absence-of-a-stromal-vascular-fraction-injection-for-osteoarthritis
#12
Phu Dinh Nguyen, Tung Dang-Xuan Tran, Huynh Ton-Ngoc Nguyen, Hieu Trung Vu, Phuong Thi-Bich Le, Nhan Lu-Chinh Phan, Ngoc Bich Vu, Ngoc Kim Phan, Phuc Van Pham
Osteoarthritis (OA) is a degenerative cartilage disease that is characterized by a local inflammatory reaction. Consequently, many studies have been performed to identify suitable prevention and treatment interventions. In recent years, both arthroscopic microfracture (AM) and stem cell therapy have been used clinically to treat OA. This study aimed to evaluate the clinical effects of AM in the presence and absence of a stromal vascular fraction (SVF) injection in the management of patients with OA. Thirty patients with grade 2 or 3 (Lawrence scale) OA of the knee participated in this study...
January 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28170170/the-infrapatellar-fat-pad-as-a-source-of-perivascular-stem-cells-with-increased-chondrogenic-potential-for-regenerative-medicine
#13
Paul Hindle, Nusrat Khan, Leela Biant, Bruno Péault
Perivascular stem cells (PSCs) are the natural ancestors of mesenchymal stem cells (MSCs) and are the stem cells responsible for homeostasis and repair in vivo. Prospectively identified and isolated PSCs have demonstrated increased plasticity and osteogenic potential. Cells from the infrapatellar fat pad (IFP) have demonstrated increased chondrogenic potential compared with those from subcutaneous fat. This research assessed the chondrogenic potential of IFP PSCs compared with MSCs from the IFP and bone marrow...
January 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28165009/acellular-urethra-bioscaffold-decellularization-of-whole-urethras-for-tissue-engineering-applications
#14
Irina N Simões, Paulo Vale, Shay Soker, Anthony Atala, Daniel Keller, Rute Noiva, Sandra Carvalho, Conceição Peleteiro, Joaquim M S Cabral, Daniel Eberli, Cláudia L da Silva, Pedro M Baptista
Patients with stress urinary incontinence mainly suffer from malfunction of the urethra closure mechanism. We established the decellularization of porcine urethras to produce acellular urethra bioscaffolds for future tissue engineering applications, using bioscaffolds or bioscaffold-derived soluble products. Cellular removal was evaluated by H&E, DAPI and DNA quantification. The presence of specific ECM proteins was assessed through immunofluorescence staining and colorimetric assay kits. Human skeletal muscle myoblasts, muscle progenitor cells and adipose-derived stromal vascular fractions were used to evaluate the recellularization of the acellular urethra bioscaffolds...
February 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28155919/obtaining-spontaneously-beating-cardiomyocyte-like-cells-from-adipose-derived-stromal-vascular-fractions-cultured-on-enzyme-crosslinked-gelatin-hydrogels
#15
Gang Yang, Zhenghua Xiao, Xiaomei Ren, Haiyan Long, Kunlong Ma, Hong Qian, Yingqiang Guo
Heart failure often develops after acute myocardial infarction because the injured myocardial tissue fails to recover or regenerate. Stem cell transplantation using adult cell sources, such as adipose-derived stromal vascular fraction (SVF), draws extensive attention. In this study, SVF cells were isolated from rat adipose tissue and cultivated on enzyme-crosslinked gelatin hydrogels. Morphological features of cell development and spontaneous beating behavior from these cells were observed and recorded. Cardiac phenotypes were characterized via immunofluorescence staining, and the expression of cardiac-specific genes was measured via RT-PCR...
February 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28152252/can-diffusion-weighted-imaging-serve-as-a-biomarker-of-fibrosis-in-pancreatic-adenocarcinoma
#16
Elizabeth M Hecht, Michael Z Liu, Martin R Prince, Sachin Jambawalikar, Helen E Remotti, Stuart W Weisberg, Donald Garmon, Sara Lopez-Pintado, Yanghee Woo, Michael D Kluger, John A Chabot
PURPOSE: To assess the relationship between diffusion-weighted imaging (DWI) and intravoxel incoherent motion (IVIM)-derived quantitative parameters (apparent diffusion coefficient [ADC], perfusion fraction [f], Dslow , diffusion coefficient [D], and Dfast , pseudodiffusion coefficient [D*]) and histopathology in pancreatic adenocarcinoma (PAC). MATERIALS AND METHODS: Subjects with suspected surgically resectable PAC were prospectively enrolled in this Health Insurance Portability and Accountability Act (HIPAA)-compliant, Institutional Review Board-approved study...
February 2, 2017: Journal of Magnetic Resonance Imaging: JMRI
https://www.readbyqxmd.com/read/28143470/current-use-of-autologous-adipose-tissue-derived-stromal-vascular-fraction-cells-for-orthopedic-applications
#17
REVIEW
Jaewoo Pak, Jung Hun Lee, Kwang Seung Park, Moonhee Park, Lin-Woo Kang, Sang Hee Lee
Autologous adipose stromal vascular fractions (SVFs) containing adipose tissue-derived stem cells (ASCs) are currently being used in clinical settings for various orthopedic applications for human patients. Due to its potential capability of regenerating cartilage, bone, and tendons, autologous adipose SVFs are being tried in treating patients with osteoarthritis (OA), chondromalacia, meniscus tear, osteonecrosis of the femoral head, and tendon injuries. Here, we have reviewed available human clinical studies with regard to patient applications of autologous adipose SVF containing ASCs, specifically assessing effectiveness and safety in the field of orthopedic disorders...
January 31, 2017: Journal of Biomedical Science
https://www.readbyqxmd.com/read/28138705/resveratrol-activates-endogenous-cardiac-stem-cells-and-improves-myocardial-regeneration-following-acute-myocardial-infarction
#18
Lin Ling, Shaohua Gu, Yan Cheng
Stem cell antigen-1-positive (Sca-1+) cardiac stem cells (CSCs) therapy for myocardial regeneration following acute myocardial infarction (AMI) is limited by insufficient cell viability and a high rate of apoptosis, due to the poor regional microenvironment. Resveratrol, which is a compound extracted from red wine, has been reported to protect myocardial tissue post‑AMI by increasing the expression of angiogenic and chemotactic factors. The present study aimed to investigate the effects of resveratrol on Sca‑1+ CSCs, and to optimize Sca‑1+ CSCs therapy for myocardial regeneration post‑AMI...
January 25, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28116724/isolation-of-mature-adipocytes-from-white-adipose-tissue-and-gene-expression-studies-by-real-time-quantitative-rt-pcr
#19
Aurelie Nguyen Dinh Cat, Ana M Briones
The study of adipose tissue and more specifically of adipocytes is considered pivotal for dissecting molecular mechanisms responsible for alterations in several organs and systems, including adipose tissue, not only in obesity but also in other diseases (hypertension, heart failure). Adipose tissue is a complex tissue composed of adipocytes and the stromal vascular fraction which includes a heterogeneous population of preadipocytes, blood cells, endothelial cells, and macrophages. In the present chapter, methods are detailed to generate purified mature adipocytes from white adipose tissue by using enzymatic digestion...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28100503/chronic-kidney-disease-promotes-chronic-inflammation-in-visceral-white-adipose-tissue
#20
Dong Mei Xiang, Xiu Zhen Song, Zhan Mei Zhou, Yang Liu, Xiao Yan Dai, Xiang Lan Huang, Fan Fan Hou, Qiu Gen Zhou
White adipose tissue plays an important role in the development of metabolic disturbance which is a common feature in patients with chronic kidney disease (CKD). The effect of CKD on white adipose tissue remains poorly appreciated. Here we evaluated the inflammatory potential of visceral white adipose tissue in a rat model of CKD. The results showed production of pro-inflammatory cytokines and infiltration of macrophage in the tissue were significantly increased in CKD rats than sham rats. Moreover, the primary adipocytes and stromal vascular fraction under the condition of CKD could trigger the inflammatory response in each other...
January 18, 2017: American Journal of Physiology. Renal Physiology
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