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Stromal vascular fraction

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https://www.readbyqxmd.com/read/29341439/therapeutic-benefits-of-cd90-negative-cardiac-stromal-cells-in-rats-with-a-30-day-chronic-infarct
#1
Deliang Shen, Miaoda Shen, Hongxia Liang, Junnan Tang, Bo Wang, Chuang Liu, Peiwen Wang, Jianzeng Dong, Ling Li, Jinying Zhang, Thomas G Caranasos
Cardiac stromal cells (CSCs) can be derived from explant cultures, and a subgroup of these cells is viewed as cardiac mesenchymal stem cells due to their expression of CD90. Here, we sought to determine the therapeutic potential of CD90-positive and CD90-negative CSCs in a rat model of chronic myocardial infarction. We obtain CD90-positive and CD90-negative fractions of CSCs from rat myocardial tissue explant cultures by magnetically activated cell sorting. In vitro, CD90-negative CSCs outperform CD90-positive CSCs in tube formation and cardiomyocyte functional assays...
January 17, 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29334574/improved-long-term-volume-retention-of-svf-gel-grafting-with-enhanced-angiogenesis-and-adipogenesis
#2
Yuteng Zhang, Junrong Cai, Tao Zhou, Yao Yao, Ziqing Dong, Feng Lu
BACKGROUND: The apoptosis of mature adipocytes after fat grafting can result in chronic inflammation, absorption, and fibrosis, leading to unpredictable outcomes. Selective elimination of mature adipocytes may result in better outcomes and a different underlying retention mode. We previously developed a mature-adipocyte-free product, SVF-gel, derived from lipoaspirate, which eliminates adipocytes while preserving the stromal vascular fraction. This study investigated the retention and regeneration mode of SVF-gel grafting...
January 10, 2018: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/29331565/saroglitazar-reduces-obesity-and-associated-inflammatory-consequences-in-murine-adipose-tissue
#3
Durgesh Kumar, Umesh Kumar Goand, Sanchita Gupta, Kripa Shankar, Salil Varshney, Sujith Rajan, Ankita Srivastava, Abhishek Gupta, Achchhe Lal Vishwakarma, Anurag Kumar Srivastava, Anil N Gaikwad
Prevailing knowledge links chronic low-grade inflammation in the adipose tissue to obesity and its associated metabolic complications. In this study, we evaluated immunometabolic effects of a recently launched dual peroxisome proliferator-activated receptor (PPAR) α & γ agonist 'Saroglitazar' in a mouse model of diet-induced obesity (DIO). Body composition analysis revealed that saroglitazar treatment promoted hepatic weight gain, while attenuated epididymal white adipose tissue (eWAT) mass in DIO. In the eWAT of saroglitazar treated mice, histological analysis showed reduced adipocyte hypertrophy and matrix deposition (picrosirius red staining)...
January 10, 2018: European Journal of Pharmacology
https://www.readbyqxmd.com/read/29324934/stem-cells-therapy-the-future-in-the-management-of-systemic-sclerosis-a-case-report
#4
Jung In Song, Silvanie Volz, Maria Eirini Liodaki, Peter Mailänder, Konstantinos Kalousis
OBJECTIVE: Systemic sclerosis (SSc) is a connective tissue disorder of unknown etiology, with heterogeneous clinical manifestations and chronic and often progressive course. The diffuse cutaneous form of SSc (dcSSc) is characterized by thickening of the skin (scleroderma) and distinctive involvement of multiple internal organs. Patients with limited cutaneous SSc (lcSSc) generally have long-standing Raynaud's phenomenon before other manifestations of SSc appear. Over the last decade the Interest of adipose-derived cell therapy in regenerative medicine has increased continuously...
September 2017: Hellenic Journal of Nuclear Medicine
https://www.readbyqxmd.com/read/29321063/first-clinical-case-report-of-local-microinjection-of-autologous-fat-and-adipose-derived-stromal-vascular-fraction-for-perianal-fistula-in-crohn-s-disease
#5
Cécile Philandrianos, Mélanie Serrero, Fanny Grimaud, Jérémy Magalon, Carine Visée, Mélanie Velier, Pauline Francois, Pierre Orsoni, Guy Magalon, Jean-Charles Grimaud, Ariadne Desjeux, Julie Véran, Florence Sabatier
Mesenchymal stem cell therapy is a promising treatment for perianal Crohn's fistulas refractory to conventional therapy, which are an extremely morbid complication and a true therapeutic challenge. Autologous adipose-derived stromal vascular fraction (ADSVF) is an easily accessible source of cells with angiogenic, anti-inflammatory, immunomodulatory, and regenerative properties. Here, we describe a case involving a patient with severe perianal Crohn's fistulas refractory to the best medical and surgical practices who received local treatment with ADSVF and microfat...
January 10, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29298894/tumor-extrinsic-discoidin-domain-receptor-1-promotes-mammary-tumor-growth-by-regulatingadipose-stromal-interleukin-6-production-in-mice
#6
Xiujie Sun, Kshama Gupta, Bogang Wu, Deyi Zhang, Bin Yuan, Xiaowen Zhang, Huai-Chin Chiang, Chi Zhang, Tyler J Curiel, Michelle P Bendeck, Stephen Hursting, Yanfen Hu, Rong Li
Discoidin domain receptor 1 (DDR1) is a collagen receptor that mediates cell communications with the extracellular matrix (ECM). Aberrant expression and activity of DDR1 in tumor cells are known to promote tumor growth. Although elevated DDR1 levels in stroma of breast tumors are associated with poor patient outcome, a causal role for tumor-extrinsic DDR1 in cancer promotion remains unclear, however. Here, we report that murine mammary tumor cells transplanted to syngeneic recipient mice in which Ddr1 has been knocked out (KO) grow less robustly than in WT mice...
January 3, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29297738/periarteriolar-glioblastoma-stem-cell-niches-express-bone-marrow-hematopoietic-stem-cell-niche-proteins
#7
Vashendriya V V Hira, Jill R Wormer, Hala Kakar, Barbara Breznik, Britt van der Swaan, Renske Hulsbos, Wikky Tigchelaar, Zbynek Tonar, Mohammed Khurshed, Remco J Molenaar, Cornelis J F Van Noorden
In glioblastoma, a fraction of malignant cells consists of therapy-resistant glioblastoma stem cells (GSCs) residing in protective niches that recapitulate hematopoietic stem cell (HSC) niches in bone marrow. We have previously shown that HSC niche proteins stromal cell-derived factor-1α (SDF-1α), C-X-C chemokine receptor type 4 (CXCR4), osteopontin (OPN), and cathepsin K (CatK) are expressed in hypoxic GSC niches around arterioles in five human glioblastoma samples. In HSC niches, HSCs are retained by binding of SDF-1α and OPN to their receptors CXCR4 and CD44, respectively...
January 1, 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/29286436/stromal-vascular-fraction-enriched-fat-grafting-for-the-treatment-of-symptomatic-end-neuromata
#8
Simon Zimmermann, Richard M Fakin, Thomas Giesen, Pietro Giovanoli, Maurizio Calcagni
The purpose of this study was to methodically illustrate and highlight the crucial steps of stromal vascular fraction (SVF)-enriched fat grafting as a novel treatment of symptomatic end-neuromata of peripheral sensory nerves, and in this study, specifically of the superficial branch of the radial nerve (SBRN). Despite a multitude of existing treatments, persistent postoperative pain and common pain relapse are still very common, independent of the procedure assessed. The neuroma is microsurgically excised accordingly to standardized protocol...
November 23, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29283192/functional-and-phenotypical-analysis-of-il-6-secreting-cd4-t-cells-in-human-adipose-tissue
#9
Anja J de Jong, Sabrina Pollastro, Joanneke C Kwekkeboom, Stefan N Andersen, Annemarie L Dorjée, Aleida M Bakker, Fawaz Alzaid, Antoine Soprani, Rob G H H Nelissen, Jan B Mullers, Nicolas Venteclef, Niek de Vries, Margreet Kloppenburg, René E M Toes, Andreea Ioan-Facsinay
Emerging evidence indicates that a dynamic interplay between the immune system and adipocytes contributes to the disturbed homeostasis in adipose tissue of obese subjects. Recently, we observed IL-6-secretion by CD4+ T cells from the stromal vascular fraction (SVF) of the infrapatellar fat pad (IFP) of knee osteoarthritis patients directly ex vivo. Here we show that human IL-6+ CD4+ T cells from SVF display a more activated phenotype than the IL-6- T cells, as evidenced by the expression of the activation marker CD69...
December 28, 2017: European Journal of Immunology
https://www.readbyqxmd.com/read/29240284/extracellular-matrix-stromal-vascular-fraction-gel-conditioned-medium-accelerates-wound-healing-in-a-murine-model
#10
Chengliang Deng, Yunfan He, Jingwei Feng, Ziqing Dong, Yao Yao, Hsiaopei Mok, Maohui Lin, Lu Feng
Conditioned medium (CM) is a new treatment modality in regenerative medicine and has shown a successful outcome in wound healing. We recently introduced extracellular matrix/stromal vascular fraction gel (ECM/SVF-gel), an adipose-derived stem cell and adipose native extracellular matrix-enriched product for cytotherapy. This study aimed to evaluate the effect of CM from ECM/SVF-gel (Gel-CM) on wound healing compared with the conventional CM from adipose tissue (Adi-CM) and stem cell (SVF-CM). In vitro wound healing effect of three CMs on keratinocytes and fibroblasts was evaluated in terms of proliferation property, migratory property, and extracellular matrix production...
December 14, 2017: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/29236575/comparing-different-nanofat-procedures-on-scars-role-of-the-stromal-vascular-fraction-and-its-clinical-implications
#11
Pietro Gentile, Giovanna Maria Scioli, Alessandra Bielli, Augusto Orlandi, Valerio Cervelli
AIM: Recently, a new fat grafting technique termed 'nanofat grafting' was proposed which improved tissue repair by the stem cells contained in the stromal vascular fraction (SVF) of nanofat. Here, we reported the clinical outcomes of different nanofat procedures in the treatment of scars in relation with SVF cell yield. METHODS: Three different modified nanofat grafting procedures (supercharged-, evo- and centrifuged-modified nanofat) were compared with the classic nanofat method, and histological analysis was performed to assess skin regeneration...
December 2017: Regenerative Medicine
https://www.readbyqxmd.com/read/29233981/a-mir-327-fgf10-fgfr2-mediated-autocrine-signaling-mechanism-controls-white-fat-browning
#12
Carina Fischer, Takahiro Seki, Sharon Lim, Masaki Nakamura, Patrik Andersson, Yunlong Yang, Jennifer Honek, Yangang Wang, Yanyan Gao, Fang Chen, Nilesh J Samani, Jun Zhang, Masato Miyake, Seiichi Oyadomari, Akihiro Yasue, Xuri Li, Yun Zhang, Yizhi Liu, Yihai Cao
Understanding the molecular mechanisms regulating beige adipocyte formation may lead to the development of new therapies to combat obesity. Here, we report a miRNA-based autocrine regulatory pathway that controls differentiation of preadipocytes into beige adipocytes. We identify miR-327 as one of the most downregulated miRNAs targeting growth factors in the stromal-vascular fraction (SVF) under conditions that promote white adipose tissue (WAT) browning in mice. Gain- and loss-of-function experiments reveal that miR-327 targets FGF10 to prevent beige adipocyte differentiation...
December 12, 2017: Nature Communications
https://www.readbyqxmd.com/read/29229387/mir-124-3p-affects-the-formation-of-intramuscular-fat-through-alterations-in-branched-chain-amino-acid-consumption-in-sheep
#13
Yangyang Pan, Jiongjie Jing, Liying Qiao, Jianhua Liu, Junxing Zhao, Lixia An, Baojun Li, Weiwei Wang, Chen Liang, Wenzhong Liu
Intramuscular fat is used to determine meat quality in animals; however, factors affecting branched chain amino acid (BCAA) catabolism, which fuels adipogenesis and lipogenesis, remain unclear. To better understand the post-transcriptional influence on BCAA catabolism during adipogenesis, we investigated the role of miR-124-3p. Stromal vascular fraction (SVF) cells were isolated from skeletal muscle of sheep, and induced to differentiate. We determined the roles of miR-124-3p and its predicted target, branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA), in adipogenic differentiation and lipogenesis of SVFs after overexpressing or inhibiting miR-124-3p or BCKDHA, respectively...
December 8, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29228092/does-stromal-vascular-fraction-supplementation-improve-facial-lipotransfer
#14
Eric Swanson
No abstract text is available yet for this article.
December 8, 2017: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/29228084/response-to-does-stromal-vascular-fraction-supplementation-improve-facial-lipotransfer
#15
Natale Ferreira Gontijo-de-Amorim, Luiz Charles-de-Sá, Gino Rigotti
No abstract text is available yet for this article.
December 8, 2017: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/29222844/effect-of-age-and-body-mass-index-on-the-yield-of-stromal-vascular-fraction
#16
Nada Alaaeddine, Oula El Atat, Nagib Saliba, Ziad Feghali, Marwan Nasr, Bilal Tarabey, George Hilal, Hussein Hashim
OBJECTIVE: The aim of this study was to determine the influence of age, body mass index, and site of liposuction on the cell yield of SVF. METHODS: A prospective study was performed on 58 patients. The average age was 39 years old, with BMI ≤ 25 or BMI ≥ 25. Fat tissue was harvested from the abdominal region, flanks, or thighs and SVF was isolated. RESULTS: The yield of viable SVF was evaluated by trypan blue, and the markers of stem cells were evaluated by flow cytometry...
December 8, 2017: Journal of Cosmetic Dermatology
https://www.readbyqxmd.com/read/29202515/recent-progresses-in-breast-reconstruction-stem-cells-biomaterials-and-growth-factors
#17
Farshad Zarei, Hadis Daraee
Breast cancer is one of the most deadly tumors in women, and new procedures for post- surgical breast reconstruction have been developed which includes autologous stromal vascular fraction (SVF), platelet-derived growth factors, biomaterials and various stem cells. Adipose derived-stem cells (ASCs) has been reported to be one of the most widely used stem cell for breast reconstruction because of their ability to form new functional adipose tissue and vessels and in combination with stromal vascular fraction has been also reported efficient for breast reconstruction...
December 4, 2017: Drug Research
https://www.readbyqxmd.com/read/29198704/ischemic-flap-survival-improvement-by-composition-selective-fat-grafting-with-novel-adipose-tissue-derived-product-stromal-vascular-fraction-gel
#18
Pan Zhang, Jingwei Feng, Yunjun Liao, Junrong Cai, Tao Zhou, Mingliang Sun, Jianhua Gao, Kai Gao
BACKGROUND: Flap necrosis due to insufficient blood supply is a common postoperative complication in random pattern flaps. Stem cell therapies have emerged as promising biologics for tissue ischemia. A novel fat derived product, stromal vascular fraction gel (SVF-gel), can be prepared with lipoaspirate through simple mechanical processing, removing only the lipid content. SVF-gel enriches adipose-derived stem cells and potentially beneficial for flap necrosis. METHODS: Nude mice ischemic flaps were treated with human SVF-gel, stromal vascular fraction (SVF) cell suspension or saline (n = 10)...
November 30, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29197029/vasculogenic-and-angiogenic-potential-of-adipose-stromal-vascular-fraction-cell-populations-in-vitro
#19
Joseph S Zakhari, Jacob Zabonick, Brian Gettler, Stuart K Williams
Adipose-derived stromal vascular fraction (SVF) is a heterogeneous cell source that contains endothelial cells, pericytes, smooth muscle cells, stem cells, and other accessory immune and stromal cells. The SVF cell population has been shown to support vasculogenesis in vitro as well vascular maturation in vivo. Matrigel, an extracellular matrix (ECM) mixture has been utilized in vitro to evaluate tube formation of purified endothelial cell systems. We have developed an in vitro system that utilizes freshly isolated SVF and ECM molecules both in pure form (fibrin, laminin, collagen) as well as premixed form (Matrigel) to evaluate endothelial tip cell formation, endothelial stalk elongation, and early stages of branching and inosculation...
December 1, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/29188676/autologous-lipotransfer-versus-stromal-vascular-fraction-enriched-lipoinjection-for-diabetic-foot-wounds-healing-a-pilot-study
#20
G Vicenti, G Solarino, V Pesce, L Moretti, A Notarnicola, M Carrozzo, F Rifino, B Moretti
Chronic ulcers of the lower limbs represent a significant social and economic burden. Diabetes is a strong risk factor for development of chronic lesions. Adult stem cells and growth factors derived from the adipose tissue are among the most promising therapeutic strategies for hard to heal wounds. Fat grafts have been used for several decades to treat soft tissue deformities, but despite its excellent characteristics, the outcome was unpredictable, due to partial necrosis and resorption of the graft. Stem cells’ enrichment of these grafts or their injection into the edges of the ulcers have shown encouraging results in various experimental settings...
October 2017: Journal of Biological Regulators and Homeostatic Agents
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