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JOURNAL ARTICLE
Tobias Huber, Verena Tripke, Janine Baumgart, Fabian Bartsch, Alicia Schulze, Stefan Weber, Stefan Heinrich, Hauke Lang
BACKGROUND: Liver surgery is the standard of care for primary and many secondary liver tumors. Due to variability and complexity in liver anatomy preoperative imaging is necessary to determine resectability and for planning the surgical strategy. In the last few years, computer-assisted resection planning has been introduced in liver surgery. Aim of this trial was the evaluation of computer-assisted three-dimensional (3D)-navigation for liver surgery. METHODS: This study was a prospective randomized-controlled pilot trial and patients were randomized in navigated or non-navigated group...
August 30, 2023: Annals of Translational Medicine
#2
JOURNAL ARTICLE
Samsuzzoha Mondal, Honey Priya James, Francesco Milano, Rui Jin, Tobias Baumgart
Bin/Amphiphysin/Rvs (BAR) proteins are known as classical membrane curvature generators during endocytosis. Amphiphysin, a member of the N-BAR sub-family of proteins that contain a characteristic amphipathic sequence at the N-terminus of the BAR domain, is involved in clathrin-mediated endocytosis. Full-length amphiphysin contains a ~ 400 amino acid long disordered linker connecting the N-BAR domain and a C-terminal Src homology 3 (SH3) domain. We express and purify recombinant amphiphysin and its N-BAR domain along with an N-terminal glutathione-S-transferase (GST) tag...
June 20, 2023: Bio-protocol
#3
JOURNAL ARTICLE
Tyler R Reagle, Yuxin Xie, Tobias Baumgart
No abstract text is available yet for this article.
February 10, 2023: Biophysical Journal
#4
JOURNAL ARTICLE
Karthik B Narayan, Samsuzzoha Mondal, Honey Priya James, Laura Baeyens, Jason Zheng, Tobias Baumgart
No abstract text is available yet for this article.
February 10, 2023: Biophysical Journal
#5
JOURNAL ARTICLE
Samsuzzoha Mondal, Karthik Narayan, Tobias Baumgart
No abstract text is available yet for this article.
February 10, 2023: Biophysical Journal
#6
REVIEW
Samsuzzoha Mondal, Tobias Baumgart
Proteins can organize into dynamic, functionally important assemblies on fluid membrane surfaces. Phase separation has emerged as an important mechanism for forming such protein assemblies on the membrane during cell signaling, endocytosis, and cytoskeleton regulation. Protein-protein phase separation thus adds novel fluid mosaics to the classical Singer and Nicolson model. Protein condensates formed in this process can modulate membrane morphologies. This is evident from recent reports of protein condensate-driven membrane reshaping in processes such as endocytosis, autophagosome formation, and protein storage vacuole morphogenesis in plants...
January 12, 2023: Biochimica et Biophysica Acta. Biomembranes
#7
JOURNAL ARTICLE
Angela Kranz, Tino Polen, Christian Kotulla, Annette Arndt, Graziella Bosco, Michael Bussmann, Ava Chattopadhyay, Annette Cramer, Cedric-Farhad Davoudi, Ursula Degner, Ramon Diesveld, Raphael Freiherr von Boeselager, Kim Gärtner, Cornelia Gätgens, Tobias Georgi, Christian Geraths, Sabine Haas, Antonia Heyer, Max Hünnefeld, Takeru Ishige, Armin Kabus, Nicolai Kallscheuer, Larissa Kever, Simon Klaffl, Britta Kleine, Martina Kočan, Abigail Koch-Koerfges, Kim J Kraxner, Andreas Krug, Aileen Krüger, Andreas Küberl, Mohamed Labib, Christian Lange, Christina Mack, Tomoya Maeda, Regina Mahr, Stephan Majda, Andrea Michel, Xenia Morosov, Olga Müller, Arun M Nanda, Jens Nickel, Jennifer Pahlke, Eugen Pfeifer, Laura Platzen, Paul Ramp, Doris Rittmann, Steffen Schaffer, Sandra Scheele, Stephanie Spelberg, Julia Schulte, Jens-Eric Schweitzer, Georg Sindelar, Ulrike Sorger-Herrmann, Markus Spelberg, Corinna Stansen, Apilaasha Tharmasothirajan, Jan van Ooyen, Philana van Summeren-Wesenhagen, Michael Vogt, Sabrina Witthoff, Lingfeng Zhu, Bernhard J Eikmanns, Marco Oldiges, Georg Schaumann, Meike Baumgart, Melanie Brocker, Lothar Eggeling, Roland Freudl, Julia Frunzke, Jan Marienhagen, Volker F Wendisch, Michael Bott
Corynebacterium glutamicum is the major host for the industrial production of amino acids and has become one of the best studied model organisms in microbial biotechnology. Rational strain construction has led to an improvement of producer strains and to a variety of novel producer strains with a broad substrate and product spectrum. A key factor for the success of these approaches is detailed knowledge of transcriptional regulation in C. glutamicum. Here, we present a large compendium of 927 manually curated microarray-based transcriptional profiles for wild-type and engineered strains detecting genome-wide expression changes of the 3,047 annotated genes in response to various environmental conditions or in response to genetic modifications...
October 1, 2022: Scientific Data
#8
JOURNAL ARTICLE
Samsuzzoha Mondal, Karthik Narayan, Samuel Botterbusch, Imania Powers, Jason Zheng, Honey Priya James, Rui Jin, Tobias Baumgart
A specific group of transmembrane receptors, including the β1-adrenergic receptor (β1-AR), is internalized through a non-clathrin pathway known as Fast Endophilin Mediated Endocytosis (FEME). A key question is: how does the endocytic machinery assemble and how is it modulated by activated receptors during FEME. Here we show that endophilin, a major regulator of FEME, undergoes a phase transition into liquid-like condensates, which facilitates the formation of multi-protein assemblies by enabling the phase partitioning of endophilin binding proteins...
August 26, 2022: Nature Communications
#9
JOURNAL ARTICLE
Tobias Huber, Laura Isabel Hanke, Christian Boedecker, Lukas Vradelis, Janine Baumgart, Stefan Heinrich, Fabian Bartsch, Jens Mittler, Alicia Schulze, Christian Hansen, Florentine Hüttl, Hauke Lang
BACKGROUND: A multitude of different diseases-benign and malign-can require surgery of the liver. The liver is an especially challenging organ for resection planning due to its unique and interindividually variable anatomy. This demands a high amount of mental imagination from the surgeon in order to plan accordingly - a skill, which takes years of training to acquire and which is difficult to teach. Since the volume of the functional remnant liver is of great importance, parenchyma sparing resections are favoured...
May 13, 2022: Trials
#10
JOURNAL ARTICLE
Rui Jin, Rui Cao, Tobias Baumgart
BAR (Bin/Amphiphysin/Rvs) domain containing proteins function as lipid bilayer benders and curvature sensors, and they contribute to membrane shaping involved in cell signaling and metabolism. The mechanism for their membrane shape sensing has been investigated by both equilibrium binding and kinetic studies. In prior research, stopped-flow spectroscopy has been used to deduce a positive dependence on membrane curvature for the binding rate constant, kon , of a BAR protein called endophilin. However, the impact of bulk diffusion of endophilin, on the kinetic binding parameters has not been thoroughly considered...
May 10, 2022: Scientific Reports
#11
JOURNAL ARTICLE
Verena Tripke, Tobias Huber, Janine Baumgart, Stefan Heinrich, Hauke Lang
BACKGROUND: Hepatic echinococcosis is rare in Germany. Liver resection is a potentially curative treatment, if a complete resection according to oncological standards can be achieved. Due to the infiltrating growth of E. multilocularis, liver resections can be challenging. CASE: We present a case of hepatic echinococcosis with complete infiltration of left and middle hepatic vein and a partial infiltration of the right hepatic vein (RHV) and the vena cava (VCI)...
May 2, 2022: Zentralblatt Für Chirurgie
#12
JOURNAL ARTICLE
Mehdi Molaei, Sreeja Kutti Kandy, Zachary T Graber, Tobias Baumgart, Ravi Radhakrishnan, John C Crocker
Lipid bilayer membranes undergo rapid bending undulations with wavelengths from tens of nanometers to tens of microns due to thermal fluctuations. Here, we probe such undulations and the membranes' mechanics by measuring the time-varying orientation of single gold nanorods (GNRs) adhered to the membrane, using high-speed dark field microscopy. In a lipid vesicle, such measurements allow the determination of the membrane's viscosity, bending rigidity, and tension as well as the friction coefficient for sliding of the monolayers over one another...
March 2022: Physical review research
#13
JOURNAL ARTICLE
Hauke Lang, Janine Baumgart, Stefan Heinrich, Tobias Huber, Lisa-Katharina Heuft, Rabea Margies, Jens Mittler, Felix Hahn, Tiemo S Gerber, Friedrich Foerster, Arndt Weinmann, Jens U Marquardt, Roman Kloeckner, Beate K Straub, Fabian Bartsch
BACKGROUND: Intrahepatic cholangiocarcinoma (iCCA) accounts for about 10% of primary liver cancer. Surgery is the only potentially curative treatment. We report on our current series of 229 consecutive hepatic resections for iCCA, which is one of the largest Western single-center series published so far. METHODS: Between January 2008 to December 2020, a total of 286 patients underwent 307 surgical explorations for intended liver resection of iCCA at our department...
August 13, 2021: Journal of Clinical Medicine
#14
JOURNAL ARTICLE
Rui Jin, Michael Grasso, Mingyang Zhou, Ronen Marmorstein, Tobias Baumgart
Endophilin, which is a member of the Bin-amphiphysin-Rvs (BAR) domain protein superfamily, contains a homodimeric N-BAR domain of a characteristic crescent shape. The N-BAR domain comprises a six-helix bundle and is known to sense and generate membrane curvature. Here, we characterize aspects of the unfolding mechanism of the endophilin A1 N-BAR domain during thermal denaturation and examine factors that influence the thermal stability of this domain. Far-UV circular dichroism (CD) spectroscopy was applied to monitor changes in the secondary structure above room temperature...
August 17, 2021: ACS Omega
#15
JOURNAL ARTICLE
Rui Jin, Tobias Baumgart
Lipid oxidation, detected in metabolic processes, is induced in excess when the cellular membrane suffers extra oxidative stress. Lipid oxidation can compromise biomembrane function in part through perturbations of lipid packing, membrane permeability, and morphology. Two major types of oxidation products, one with a partially truncated lipid tail with a hydrophilic group at the tail-end, and secondly, a lysolipid (with one of the chains completely truncated) can disturb the membrane bilayer packing significantly...
August 28, 2021: Soft Matter
#16
JOURNAL ARTICLE
Samuel Botterbusch, Tobias Baumgart
Liquid-liquid phase separation has recently emerged as an important fundamental organizational phenomenon in biological settings. Most studies of biological phase separation have focused on droplets that "condense" from solution above a critical concentration, forming so-called "membraneless organelles" suspended in solution. However, membranes are ubiquitous throughout cells, and many biomolecular condensates interact with membrane surfaces. Such membrane-associated phase-separated systems range from clusters of integral or peripheral membrane proteins in the plane of the membrane to free, spherical droplets wetting membrane surfaces to droplets containing small lipid vesicles...
February 2021: Applied Sciences
#17
JOURNAL ARTICLE
Jaclyn Robustelli, Tobias Baumgart
Endophilin is an N-BAR protein, which is characterized by a crescent-shaped BAR domain and an amphipathic helix that contributes to the membrane binding of these proteins. The exact function of that H0 helix has been a topic of debate. In mammals, there are five different endophilin isoforms, grouped into A (three members) and B (two members) subclasses, which have been described to differ in their subcellular localization and function. We asked to what extent molecular properties of the H0 helices of these members affect their membrane targeting behavior...
October 1, 2021: Biochimica et Biophysica Acta. Biomembranes
#18
JOURNAL ARTICLE
Christian Boedecker, Florentine Huettl, Patrick Saalfeld, Markus Paschold, Werner Kneist, Janine Baumgart, Bernhard Preim, Christian Hansen, Hauke Lang, Tobias Huber
PURPOSE: Three-dimensional (3D) surgical planning is widely accepted in liver surgery. Currently, the 3D reconstructions are usually presented as 3D PDF data on regular monitors. 3D-printed liver models are sometimes used for education and planning. METHODS: We developed an immersive virtual reality (VR) application that enables the presentation of preoperative 3D models. The 3D reconstructions are exported as STL files and easily imported into the application, which creates the virtual model automatically...
May 2021: Langenbeck's Archives of Surgery
#19
JOURNAL ARTICLE
Clara Lemos, Volker K Schulze, Simon J Baumgart, Ekaterina Nevedomskaya, Tobias Heinrich, Julien Lefranc, Benjamin Bader, Clara D Christ, Hans Briem, Lara P Kuhnke, Simon J Holton, Ulf Bömer, Philip Lienau, Franz von Nussbaum, Carl F Nising, Marcus Bauser, Andrea Hägebarth, Dominik Mumberg, Bernard Haendler
PURPOSE: 5' adenosine monophosphate-activated kinase (AMPK) is an essential regulator of cellular energy homeostasis and has been associated with different pathologies, including cancer. Precisely defining the biological role of AMPK necessitates the availability of a potent and selective inhibitor. METHODS: High-throughput screening and chemical optimization were performed to identify a novel AMPK inhibitor. Cell proliferation and mechanistic assays, as well as gene expression analysis and chromatin immunoprecipitation were used to investigate the cellular impact as well as the crosstalk between lipid metabolism and androgen signaling in prostate cancer models...
June 2021: Cellular Oncology (Dordrecht)
#20
JOURNAL ARTICLE
Su Jung Oh-Hohenhorst, Derya Tilki, Ann-Kristin Ahlers, Anna Suling, Oliver Hahn, Pierre Tennstedt, Christiane Matuszcak, Hanna Maar, Vera Labitzky, Sandra Hanika, Sarah Starzonek, Simon Baumgart, Steven A Johnsen, Martina Kluth, Hüseyin Sirma, Ronald Simon, Guido Sauter, Hartwig Huland, Udo Schumacher, Tobias Lange
The outcome of prostate cancer (PCa) patients is highly variable and depends on whether or not distant metastases occur. Multiple chromosomal deletions have been linked to early tumor marker PSA recurrence (biochemical relapse, BCR) after radical prostatectomy (RP), but their potential role for distant metastasis formation is largely unknown. Here, we specifically analyzed whether deletion of the tumor suppressor CHD1 (5q21) influences the post-surgical risk of distant metastasis and whether CHD1 loss directly contributes to metastasis formation in vivo...
January 2022: Cancer Gene Therapy
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