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Chilling requirement

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https://www.readbyqxmd.com/read/28893508/regulation-of-protein-phosphatase-2a-during-embryonic-diapause-process-in-the-silkworm-bombyx-mori
#1
Shi-Hong Gu, Hsiao-Yen Hsieh, Pei-Ling Lin
Regulation of protein phosphorylation requires coordinated interactions between protein kinases and protein phosphatases. In the present study, we investigated regulation of protein phosphatase 2A (PP2A) during the embryonic diapause process of B. mori. An immunobloting analysis showed that Bombyx eggs contained a catalytic C subunit, a major 55-kDa regulatory B subunit (B55/PR55 subunit), and a structural A subunit, with the A and B subunits undergoing differential changes between diapause and non-diapause eggs during embryonic process...
September 8, 2017: Journal of Insect Physiology
https://www.readbyqxmd.com/read/28861091/insufficient-chilling-effects-vary-among-boreal-tree-species-and-chilling-duration
#2
Rongzhou Man, Pengxin Lu, Qing-Lai Dang
Insufficient chilling resulting from rising winter temperatures associated with climate warming has been an area of particular interest in boreal and temperate regions where a period of cool temperatures in fall and winter is required to break plant dormancy. In this study, we examined the budburst and growth of trembling aspen (Populus tremuloides Michx.), balsam poplar (Populus balsamifera L.), white birch (Betula papyrifera Marsh.), black spruce (Picea mariana (Mill.) B.S.P.), white spruce (Picea glauca (Moench) Voss), jack pine (Pinus banksiana Lamb...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28828598/humidity-does-not-appear-to-trigger-leaf-out-in-woody-plants
#3
Lucy Zipf, Richard B Primack
In order to anticipate the ecological impacts of climate change and model changes to forests, it is important to understand the factors controlling spring leaf out. Leaf out phenology in woody trees and shrubs is generally considered to be strongly controlled by a combination of spring warming, winter chilling requirement, and photoperiod. However, researchers have recently suggested that temperature-related air humidity, rather than temperature itself, might be the main trigger of the spring leaf-out of woody plants...
August 21, 2017: International Journal of Biometeorology
https://www.readbyqxmd.com/read/28817647/caprine-brucellosis-a-historically-neglected-disease-with-significant-impact-on-public-health
#4
REVIEW
Carlos A Rossetti, Angela M Arenas-Gamboa, Estefanía Maurizio
Caprine brucellosis is a chronic infectious disease caused by the gram-negative cocci-bacillus Brucella melitensis. Middle- to late-term abortion, stillbirths, and the delivery of weak offspring are the characteristic clinical signs of the disease that is associated with an extensive negative impact in a flock's productivity. B. melitensis is also the most virulent Brucella species for humans, responsible for a severely debilitating and disabling illness that results in high morbidity with intermittent fever, chills, sweats, weakness, myalgia, abortion, osteoarticular complications, endocarditis, depression, anorexia, and low mortality...
August 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28759789/changes-in-carbohydrate-levels-and-relative-water-content-rwc-to-distinguish-dormancy-phases-in-sweet-cherry
#5
Heiko Kaufmann, Michael Blanke
Perennial trees require chilling, i.e. a period of cold temperature in the winter, for flowering next spring; sweet cherry is particularly prone to lack of chilling. The objective of this study is to identify possible transition points to clearly distinguish dormancy phases by relating carbohydrate and relative water content (RWC) in reproductive buds to concomitant chilling fulfilment. This contribution proposes the use of four transition points between the dormancy phases and their characterization in terms of carbohydrates, water contents in combination with chilling values and may allow upscaling to other dormancy studies in trees; two groups of cherry varieties were defined based on their different initial sorbitol and starch level in the autumn...
July 19, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28716048/transcriptome-analysis-uncovers-arabidopsis-f-box-stress-induced-1-as-a-regulator-of-jasmonic-acid-and-abscisic-acid-stress-gene-expression
#6
Lauren E Gonzalez, Kristen Keller, Karen X Chan, Megan M Gessel, Bryan C Thines
BACKGROUND: The ubiquitin 26S proteasome system (UPS) selectively degrades cellular proteins, which results in physiological changes to eukaryotic cells. F-box proteins are substrate adaptors within the UPS and are responsible for the diversity of potential protein targets. Plant genomes are enriched in F-box genes, but the vast majority of these have unknown roles. This work investigated the Arabidopsis F-box gene F-BOX STRESS INDUCED 1 (FBS1) for its effects on gene expression in order elucidate its previously unknown biological function...
July 17, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28708517/factors-affecting-the-reproductive-performance-of-bitches-a-prospective-cohort-study-involving-1203-inseminations-with-fresh-and-frozen-semen
#7
F K Hollinshead, D W Hanlon
The aim of this prospective cohort study was to utilize multivariable statistical methods to identify factors that significantly affected whelping rate, litter size and gestation length in a large population of bitches of many different breeds, presented for routine breeding management. In addition, we aimed to determine the incidence of dystocia and the proportion of bitches undergoing a caesarean section procedure. A total of 1146 individual bitches representing 84 different breeds contributed 1203 inseminations over the 9 year (2007-2015) study period...
June 22, 2017: Theriogenology
https://www.readbyqxmd.com/read/28707409/chilling-responsive-demeter-like-dna-demethylase-mediates-in-poplar-bud-break
#8
Daniel Conde, Anne-Laure Le Gac, Mariano Perales, Christopher Dervinis, Matias Kirst, Stéphane Maury, Pablo González-Melendi, Isabel Allona
Annual dormancy-growth cycle is a developmental and physiological process essential for the survival of deciduous trees in temperate and boreal forests. Seasonal control of shoot growth in woody perennials requires specific genetic programmes responding to environmental signals. The environmental-controlled mechanisms that regulate the shift between winter dormancy and the growth-promoting genetic programmes are still unknown. Here, we show that dynamics in genomic DNA methylation levels are involved in the regulation of dormancy-growth cycle in poplar...
October 2017: Plant, Cell & Environment
https://www.readbyqxmd.com/read/28698472/oxidative-stress-associated-with-chilling-injury-in-immature-fruit-postharvest-technological-and-biotechnological-solutions
#9
REVIEW
Juan Luis Valenzuela, Susana Manzano, Francisco Palma, Fátima Carvajal, Dolores Garrido, Manuel Jamilena
Immature, vegetable-like fruits are produced by crops of great economic importance, including cucumbers, zucchini, eggplants and bell peppers, among others. Because of their high respiration rates, associated with high rates of dehydration and metabolism, and their susceptibility to chilling injury (CI), vegetable fruits are highly perishable commodities, requiring particular storage conditions to avoid postharvest losses. This review focuses on the oxidative stress that affects the postharvest quality of vegetable fruits under chilling storage...
July 8, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28694818/insights-into-the-molecular-events-that-regulate-heat-induced-chilling-tolerance-in-citrus-fruits
#10
María T Lafuente, Beatriz Establés-Ortíz, Luis González-Candelas
Low non-freezing temperature may cause chilling injury (CI), which is responsible for external quality deterioration in many chilling-sensitive horticultural crops. Exposure of chilling-sensitive citrus cultivars to non-lethal high-temperature conditioning may increase their chilling tolerance. Very little information is available about the molecular events involved in such tolerance. In this work, the molecular events associated with the low temperature tolerance induced by heating Fortune mandarin, which is very sensitive to chilling, for 3 days at 37°C prior to cold storage is presented...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28666130/tracking-of-listeria-monocytogenes-in-meat-establishment-using-whole-genome-sequencing-as-a-food-safety-management-tool-a-proof-of-concept
#11
Ivan Nastasijevic, Dubravka Milanov, Branko Velebit, Vesna Djordjevic, Craig Swift, Anais Painset, Brankica Lakicevic
Repeated Listeria outbreaks particularly associated with Ready-To-Eat (RTE) delicatessen meat products have been reported annually at global level. The most frequent scenario that led to foodborne outbreaks was the post-thermal treatment cross-contamination of deli meat products during slicing and modified atmosphere packaging (MAP). The precondition for such cross contamination is the previous introduction of Listeria into meat processing facilities and subsequent colonization of the production environment, associated with formation of biofilms resilient to common sanitation procedures regularly applied in meat establishments...
June 20, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28629320/transcriptomic-changes-reveal-gene-networks-responding-to-the-overexpression-of-a-blueberry-dwarf-and-delayed-flowering-1-gene-in-transgenic-blueberry-plants
#12
Guo-Qing Song, Xuan Gao
BACKGROUND: Constitutive expression of the CBF/DREB1 for increasing freezing tolerance in woody plants is often associated with other phenotypic changes including dwarf plant and delayed flowering. These phenotypic changes have been observed when Arabidopsis DWARF AND DELAYED FLOWERING 1 (DDF1) was overexpressed in A. thaliana plants. To date, the DDF1 orthologues have not been studied in woody plants. The aim of this study is to investigate transcriptomic responses to the overexpression of blueberry (Vaccinium corymbosum) DDF1 (herein, VcDDF1-OX)...
June 19, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28601423/crohn-disease-epidemiology-diagnosis-and-management
#13
REVIEW
Joseph D Feuerstein, Adam S Cheifetz
Crohn disease is a chronic idiopathic inflammatory bowel disease condition characterized by skip lesions and transmural inflammation that can affect the entire gastrointestinal tract from the mouth to the anus. For this review article, we performed a review of articles in PubMed through February 1, 2017, by using the following Medical Subject Heading terms: crohns disease, crohn's disease, crohn disease, inflammatory bowel disease, and inflammatory bowel diseases. Presenting symptoms are often variable and may include diarrhea, abdominal pain, weight loss, nausea, vomiting, and in certain cases fevers or chills...
July 2017: Mayo Clinic Proceedings
https://www.readbyqxmd.com/read/28588368/two-cases-of-esophageal-injury-following-anterior-cervical-discectomy-and-fusion-one-overt-and-one-covert
#14
Prasad Krishnan, Rajaraman Kartikueyan, Sachinkumar Maheshbhai Patel, Partha Pratim Bose, Kanchan Kumar Mukherjee
Esophageal perforation is a dreaded complication of anterior cervical spinal surgery. A 52-year-old diabetic man had undergone a surgery for a C6-C7 disc prolapse and developed spiking fever with chills and rigor on the 7th postoperative day. No cause could be found out but a CT scan of thorax done in the course of investigations revealed pneumomediastimum. The patient succumbed on the 10th day after surgery. Autopsy revealed the cause of death to be mediastinitis following iatrogenic esophageal perforation...
May 2017: Annals of Neurosciences
https://www.readbyqxmd.com/read/28549208/identification-of-reference-genes-for-rt-qpcr-analysis-in-peach-genotypes-with-contrasting-chilling-requirements
#15
N Marini, C B Bevilacqua, M V Büttow, M C B Raseira, S Bonow
Selecting and validating reference genes are the first steps in studying gene expression by reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR). The present study aimed to evaluate the stability of five reference genes for the purpose of normalization when studying gene expression in various cultivars of Prunus persica with different chilling requirements. Flower bud tissues of nine peach genotypes from Embrapa's peach breeding program with different chilling requirements were used, and five candidate reference genes based on the RT-qPCR that were useful for studying the relative quantitative gene expression and stability were evaluated using geNorm, NormFinder, and bestKeeper software packages...
May 25, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28548487/the-challenge-of-overcoming-antibiotic-resistance-an-adjuvant-approach
#16
Roberta J Melander, Christian Melander
Antibiotic resistance is one of the greatest current threats to human health, and without significant action we face the chilling prospect of a world without effective antibiotics. Although continued effort toward the development of new antibiotics, particularly those with novel mechanisms of action, remains crucial, this alone probably will not be enough to prevail, and it is imperative that additional approaches are also explored. One such approach is the identification of adjuvants that augment the activity of current antibiotics...
May 26, 2017: ACS Infectious Diseases
https://www.readbyqxmd.com/read/28541567/dna-methylation-and-small-interference-rnas-participate-in-the-regulation-of-mads-box-genes-involved-in-dormancy-in-sweet-cherry-prunus-avium-l
#17
Karin Rothkegel, Evelyn Sánchez, Christian Montes, Macarena Greve, Sebastián Tapia, Soraya Bravo, Humberto Prieto, Andréa Miyasaka Almeida
Epigenetic modifications can yield information about connections between genotype, phenotype variation and environmental conditions. Bud dormancy release in temperate perennial fruit trees depends on internal and environmental signals such as cold accumulation and photoperiod. Previous investigations have noted the participation of epigenetic mechanisms in the control of this physiological process. We examined whether epigenetic modifications were modulated in MADS-box genes, potential candidates for the regulation of bud dormancy and flowering in sweet cherry (Prunus avium L...
May 24, 2017: Tree Physiology
https://www.readbyqxmd.com/read/28533930/early-onset-lyme-carditis-with-concurrent-disseminated-erythema-migrans
#18
Kinjan P Patel, Peter D Farjo, Joy J Juskowich, Ali Hama Amin, James D Mills
BACKGROUND: Lyme disease is an infection that is estimated to affect over 300,000 people in the United States annually. Typically, it presents with erythema migrans (EM), an annular rash at the site of tick attachment, within 3 to 30 days of inoculation. Untreated patients may progress to early disseminated disease. A further complication, Lyme carditis is rare but may occur several weeks later. It commonly manifests as a variable atrioventricular (AV) conduction block, with a high-grade AV block occurring in only 1% of untreated patients...
2017: American Journal of Cardiovascular Disease
https://www.readbyqxmd.com/read/28495891/trimethylguanosine-synthase1-tgs1-is-essential-for-chilling-tolerance
#19
Jinpeng Gao, James G Wallis, Jeremy B Jewell, John Browse
Chilling stress is a major factor limiting plant development and crop productivity. Because the plant response to chilling is so complex, we are far from understanding the genes important in the response to chilling. To identify new genes important in chilling tolerance, we conducted a novel mutant screen, combining a confirmed SALK T-DNA insertion collection with traditional forward genetics. We screened a pool of more than 3700 confirmed homozygous SALK T-DNA insertion lines for visible defects under prolonged growth at 5°C...
July 2017: Plant Physiology
https://www.readbyqxmd.com/read/28473914/isolated-splenic-abscess-in-brucellosis
#20
Sreenivasa Rao Sudulagunta, Monica Kumbhat, Mahesh Babu Sodalagunta, Aravinda Settikere Nataraju, Shiva Kumar Bangalore Raja
Brucellosis is a zoonotic infectious disease, which can attack any organ of the body but mainly involves lymphoreticular system. Our case report describes isolated splenic abscess diagnosed in a 50-year-old individual who is a milk vendor by occupation and has the habit of consuming raw milk. He was admitted with pain abdomen, high-grade fever with chills, generalized malaise, night sweats and weight loss. Ultrasound and computerized tomography of the abdomen showed splenomegaly and hypodense, nonenhancing lesion measuring 3...
February 2017: Oxford Medical Case Reports
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