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https://www.readbyqxmd.com/read/28917199/enhanced-phosphorus-and-ciprofloxacin-removal-in-a-modified-baf-system-by-configuring-fe-c-micro-electrolysis-investigation-on-pollutants-removal-and-degradation-mechanisms
#1
Longlong Zhang, Qinyan Yue, Kunlun Yang, Pin Zhao, Baoyu Gao
A modified biological aerated filter (BAF) system configured Fe-C micro electrolysis was applied to enhance phosphorus and ciprofloxacin (CIP) removal. A novel sludge ceramic and sintering ferric-carbon ceramic (SFC) were separately packed into a lab-scale BAF and Fe-C micro electrolysis reactor. The BAF and Fe-C micro electrolysis coupled system was operated about 230days. The enhancement of phosphorus and ciprofloxacin removals by Fe-C micro electrolysis, the degradation mechanisms of CIP and the variations of microbial population were investigated...
September 8, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28916764/rapid-and-reversible-epigenome-editing-by-endogenous-chromatin-regulators
#2
Simon M G Braun, Jacob G Kirkland, Emma J Chory, Dylan Husmann, Joseph P Calarco, Gerald R Crabtree
Understanding the causal link between epigenetic marks and gene regulation remains a central question in chromatin biology. To edit the epigenome we developed the FIRE-Cas9 system for rapid and reversible recruitment of endogenous chromatin regulators to specific genomic loci. We enhanced the dCas9-MS2 anchor for genome targeting with Fkbp/Frb dimerizing fusion proteins to allow chemical-induced proximity of a desired chromatin regulator. We find that mSWI/SNF (BAF) complex recruitment is sufficient to oppose Polycomb within minutes, leading to activation of bivalent gene transcription in mouse embryonic stem cells...
September 15, 2017: Nature Communications
https://www.readbyqxmd.com/read/28898675/a-dna-crosslinker-collects-mitotic-chromosomes
#3
Mingxuan Sun, Rebecca Heald
Incorporating each set of daughter chromosomes into a single nucleus at the end of mitosis is essential for genome stability. In a recent Cell paper, Samwer et al. (2017) show that by non-covalently crosslinking DNA, BAF promotes chromosome coalescence, preventing nuclear membranes from enwrapping individual chromosomes to form micronuclei.
September 11, 2017: Developmental Cell
https://www.readbyqxmd.com/read/28891954/assessment-of-heavy-metal-pollution-and-health-risks-in-the-soil-plant-human-system-in-the-yangtze-river-delta-china
#4
Bifeng Hu, Xiaolin Jia, Jie Hu, Dongyun Xu, Fang Xia, Yan Li
Heavy metal (HM) contamination and accumulation is a serious problem around the world due to the toxicity, abundant sources, non-biodegradable properties, and accumulative behaviour of HMs. The degree of soil HM contamination in China, especially in the Yangtze River Delta, is prominent. In this study, 1822 pairs of soil and crop samples at corresponding locations were collected from the southern Yangtze River Delta of China, and the contents of Ni, Cr, Zn, Cd, As, Cu, Hg, and Pb were measured. The single pollution index in soil (SPI) and Nemerow composite pollution index (NCPI) were used to assess the degree of HM pollution in soil, and the crop pollution index (CPI) was used to explore the degree of HM accumulation in crops...
September 10, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28882431/abnormal-retention-of-nuclear-lamina-and-disorganization-of-chromatin-related-proteins-in-spermatozoa-from-dpy19l2-deleted-globozoospermic-patients
#5
Marine Paci, Razan Elkhatib, Guy Longepied, Sylviane Hennebicq, Julien Bessonat, Blandine Courbière, Patrice Bourgeois, Nicolas Levy, Michael J Mitchell, Catherine Metzler-Guillemain
The aim of this study was to characterize the nuclear lamina (NL) and lamin chromatin-partners in spermatozoa from four DPY19L2-deleted globozoospermic patients. We tested for spermatid transcripts encoding lamins and their chromatin-partners emerin, LAP2α, BAF and BAF-L, by reverse transcriptase-PCR using spermatozoa RNA. We also determined the localization of lamin B1, BAF and BAF-L by immunofluorescent analysis of spermatozoa from all patients. In RNA from globozoospermic and control spermatozoa we detected transcripts encoding lamin B1, lamin B3, emerin, LAP2α and BAF-L, but not A-type lamins...
August 4, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28875991/nuclear-envelope-chromosomes-one-baf-layer-to-bind-them-all
#6
Paulina Strzyz
No abstract text is available yet for this article.
October 2017: Nature Reviews. Molecular Cell Biology
https://www.readbyqxmd.com/read/28873082/ffar2-expression-regulates-leukaemic-cell-growth-in-vivo
#7
Laure B Bindels, Paolo E Porporato, Sarah Ducastel, Martina Sboarina, Audrey M Neyrinck, Evelyne M Dewulf, Olivier Feron, Sophie Lestavel, Patrice D Cani, Bart Staels, Pierre Sonveaux, Nathalie M Delzenne
BACKGROUND: Activation of free fatty acid receptor 2 (FFAR2) by microbiota-derived metabolites (e.g., propionate) reduces leukaemic cell proliferation in vitro. This study aims to test whether Ffar2 expression per se also influences leukaemia cell growth in vivo. METHODS: Bcr-Abl-expressing BaF cells were used as a leukaemia model and the role of Ffar2 was evaluated in Balb/c mice after lentiviral shRNA transduction. RESULTS: Our data formally establish that reduced leukaemic cell proliferation is associated with increased Ffar2 expression in vivo and in vitro...
September 5, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/28864478/ews-fli1-retargets-baf-chromatin-remodeling-complexes-in-ewing-sarcoma
#8
(no author information available yet)
BAF recruitment to GGAA microsatellites activates the Ewing sarcoma transcriptional program.
September 1, 2017: Cancer Discovery
https://www.readbyqxmd.com/read/28858706/comparative-investigation-of-toxicity-and-bioaccumulation-of-cd-based-quantum-dots-and-cd-salt-in-freshwater-plant-lemna-minor-l
#9
Pavlína Modlitbová, Karel Novotný, Pavel Pořízka, Jakub Klus, Přemysl Lubal, Helena Zlámalová-Gargošová, Jozef Kaiser
The purpose of this study was to determine the toxicity of two different sources of cadmium, i.e. CdCl2 and Cd-based Quantum Dots (QDs), for freshwater model plant Lemna minor L. Cadmium telluride QDs were capped with two coating ligands: glutathione (GSH) or 3-mercaptopropionic acid (MPA). Growth rate inhibition and final biomass inhibition of L. minor after 168-h exposure were monitored as toxicity endpoints. Dose-response curves for Cd toxicity and EC50168h values were statistically evaluated for all sources of Cd to uncover possible differences among the toxicities of tested compounds...
August 28, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28854392/transport-of-pharmaceuticals-and-their-metabolites-between-water-and-sediments-as-a-further-potential-exposure-for-aquatic-organisms
#10
Olga Koba, Katerina Grabicova, Daniel Cerveny, Jan Turek, Jitka Kolarova, Tomas Randak, Vladimir Zlabek, Roman Grabic
Although pharmaceuticals are frequently studied contaminants, their fate in the environment is still not completely clear. During a one year study, a complex approach including water, sediment and fish sampling was used to describe the behaviour of pharmaceuticals and their metabolites (PTMs) in the environment. Eighteen pharmaceuticals and seven of their metabolites were determined in a pond used for the tertiary treatment of wastewater effluent. A liquid chromatography-tandem mass spectrometry method was applied to determine the PTMs concentrations in all matrices...
August 23, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28844864/the-short-isoform-of-brd4-promotes-hiv-1-latency-by-engaging-repressive-swi-snf-chromatin-remodeling-complexes
#11
Ryan J Conrad, Parinaz Fozouni, Sean Thomas, Hendrik Sy, Qiang Zhang, Ming-Ming Zhou, Melanie Ott
BET proteins commonly activate cellular gene expression, yet inhibiting their recruitment paradoxically reactivates latent HIV-1 transcription. Here we identify the short isoform of BET family member BRD4 (BRD4S) as a corepressor of HIV-1 transcription. We found that BRD4S was enriched in chromatin fractions of latently infected T cells, and it was more rapidly displaced from chromatin upon BET inhibition than the long isoform. BET inhibition induced marked nucleosome remodeling at the latent HIV-1 promoter, which was dependent on the activity of BRG1-associated factors (BAF), an SWI/SNF chromatin-remodeling complex with known repressive functions in HIV-1 transcription...
September 21, 2017: Molecular Cell
https://www.readbyqxmd.com/read/28844694/cancer-specific-retargeting-of-baf-complexes-by-a-prion-like-domain
#12
Gaylor Boulay, Gabriel J Sandoval, Nicolo Riggi, Sowmya Iyer, Rémi Buisson, Beverly Naigles, Mary E Awad, Shruthi Rengarajan, Angela Volorio, Matthew J McBride, Liliane C Broye, Lee Zou, Ivan Stamenkovic, Cigall Kadoch, Miguel N Rivera
Alterations in transcriptional regulators can orchestrate oncogenic gene expression programs in cancer. Here, we show that the BRG1/BRM-associated factor (BAF) chromatin remodeling complex, which is mutated in over 20% of human tumors, interacts with EWSR1, a member of a family of proteins with prion-like domains (PrLD) that are frequent partners in oncogenic fusions with transcription factors. In Ewing sarcoma, we find that the BAF complex is recruited by the EWS-FLI1 fusion protein to tumor-specific enhancers and contributes to target gene activation...
September 21, 2017: Cell
https://www.readbyqxmd.com/read/28841419/dna-cross-bridging-shapes-a-single-nucleus-from-a-set-of-mitotic-chromosomes
#13
Matthias Samwer, Maximilian W G Schneider, Rudolf Hoefler, Philipp S Schmalhorst, Julian G Jude, Johannes Zuber, Daniel W Gerlich
Eukaryotic cells store their chromosomes in a single nucleus. This is important to maintain genomic integrity, as chromosomes packaged into separate nuclei (micronuclei) are prone to massive DNA damage. During mitosis, higher eukaryotes disassemble their nucleus and release individualized chromosomes for segregation. How numerous chromosomes subsequently reform a single nucleus has remained unclear. Using image-based screening of human cells, we identified barrier-to-autointegration factor (BAF) as a key factor guiding membranes to form a single nucleus...
August 24, 2017: Cell
https://www.readbyqxmd.com/read/28827547/tracing-heavy-metals-in-swine-manure-maggot-chicken-production-chain
#14
Wanqiang Wang, Wenjuan Zhang, Xiaoping Wang, Chaoliang Lei, Rui Tang, Feng Zhang, Qizhi Yang, Fen Zhu
With the development of large-scale livestock farming, manure pollution has drawn much attention. Conversion by insects is a rapid and cost-effective new method for manure management. Swine manure conversion with maggots (Musca domestica larvae) has developed, and the harvested maggots are often used as animal feed. However, the flow of heavy metals from manure to downstream processes cannot be ignored, and therefore, heavy metal content was measured in untreated raw manure, maggot-treated manure, harvested maggots and maggot-eating chickens (chest muscle and liver) to evaluate potential heavy metal risks...
August 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28825984/bioaccumulation-of-psychoactive-pharmaceuticals-in-fish-in-an-effluent-dominated-stream
#15
Katerina Grabicova, Roman Grabic, Ganna Fedorova, Jerker Fick, Daniel Cerveny, Jitka Kolarova, Jan Turek, Vladimir Zlabek, Tomas Randak
The treated effluent from sewage treatment plants (STP) is a major source of active pharmaceutical ingredients (APIs) that enter the aquatic environment. Bioaccumulation of 11 selected psychoactive pharmaceuticals (citalopram, clomipramine, haloperidol, hydroxyzine, levomepromazine, mianserin, mirtazapine, paroxetine, sertraline, tramadol and venlafaxine) was examined in Zivny Stream (tributary of the Blanice River, the Czech Republic), which is a small stream highly affected by effluent from the Prachatice STP...
August 8, 2017: Water Research
https://www.readbyqxmd.com/read/28819831/biodiversity-variability-and-metal-accumulation-strategies-in-plants-spontaneously-inhibiting-fly-ash-lagoon-india
#16
Suchita Mukhopadhyay, Vivek Rana, Adarsh Kumar, Subodh Kumar Maiti
Out of 29 plant species taken into consideration for biodiversity investigations, the present study screened out Cyperus rotundus L., Calotropis procera (Aiton) W.T. Aiton, Croton bonplandianus Baill., Eclipta prostrata (L.) L., and Vernonia cinerea (L.) Less. as the most suitable metal-tolerant plant species (high relative density and frequency) which can grow on metal-laden fly ash (FA) lagoon. Total (aqua-regia), residual (HNO3) and plant available (CaCl2) metal concentrations were assessed for the clean-up of metal-contaminated FA disposal site using naturally colonized plants...
August 18, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28801106/comparison-of-caged-and-native-blue-mussels-mytilus-edulis-spp-for-environmental-monitoring-of-pah-pcb-and-trace-metals
#17
Merete Schøyen, Ian J Allan, Anders Ruus, Jarle Håvardstun, Dag Ø Hjermann, Jonny Beyer
Contaminant bioaccumulation was studied in blue mussels (Mytilus edulis spp.) using the harbor waters of Kristiansand (Norway) as a case study. A suite of chemical contaminants (trace metals, PAHs and PCBs) was analyzed in caged and native mussels as well as in passive samplers (Diffusive Gradients in Thin films (DGT)-devices and silicone rubbers) placed alongside the mussels for estimation of contaminant concentrations in water and uptake rates and bioaccumulation factors (BAFs) in mussels during a six-months deployment period...
August 2, 2017: Marine Environmental Research
https://www.readbyqxmd.com/read/28783015/application-of-novel-modified-biological-aerated-filter-mbaf-as-a-promising-post-treatment-for-water-reuse-modification-in-configuration-and-backwashing-process
#18
Ali Nikoonahad, Mohammad Taghi Ghaneian, Amir Hossein Mahvi, Mohammad Hassan Ehrampoush, Ali Asghar Ebrahimi, Mohammad Hassan Lotfi, Sima Salamehnejad
Biological Aerated Filter (BAF) reactors due to their plentiful biomass, high shockability, high efficiency, good filtration, availability and lack of need for large land areas, are enjoying from great importance in advanced wastewater treatment. Therefore, in this study, Polystyrene Coated by Sand (PCS) was produced as a novel media and its application in a modified down-flow BAF structure for advanced wastewater treatment was assessed in two steps. In step one, the backwash effluent did not return to the system, while in step two backwash effluent returned to increase the water reuse efficiency...
August 3, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28774089/on-the-sr1-xbaxfeo%C3%A2-f-oxyfluoride-perovskites-structure-and-magnetism-from-neutron-diffraction-and-m%C3%A3-ssbauer-spectroscopy
#19
Crisanto A García-Ramos, María Retuerto, José Antonio Alonso
Four oxyfluorides of the title series (x = 0.00, 0.25, 0.50, 0.75) have been stabilized by topotactic treatment of perovskite precursors Sr1-xBaxFeO3-δ prepared by soft-chemistry procedures, yielding reactive materials that can easily incorporate a substantial amount of F atoms at moderate temperatures, thus avoiding the stabilization of competitive SrF₂ and BaF₂ parasitic phases. XRD and Neutron Powder Diffraction (NPD) measurements assess the phase purity and yield distinct features concerning the unit cell parameters' variation, the Sr and Ba distribution, the stoichiometry of the anionic sublattice and the anisotropic displacement factors for O and F atoms...
November 29, 2016: Materials
https://www.readbyqxmd.com/read/28737272/dynamic-coupling-between-fmri-local-connectivity-and-interictal-eeg-in-focal-epilepsy-a-wavelet-analysis-approach
#20
Amir Omidvarnia, Mangor Pedersen, David N Vaughan, Jennifer M Walz, David F Abbott, Andrew Zalesky, Graeme D Jackson
Simultaneous scalp EEG-fMRI recording is a noninvasive neuroimaging technique for combining electrophysiological and hemodynamic aspects of brain function. Despite the time-varying nature of both measurements, their relationship is usually considered as time-invariant. The aim of this study was to detect direct associations between scalp-recorded EEG and regional changes of hemodynamic brain connectivity in focal epilepsy through a time-frequency paradigm. To do so, we developed a voxel-wise framework that analyses wavelet coherence between dynamic regional phase synchrony (DRePS, calculated from fMRI) and band amplitude fluctuation (BAF) of a target EEG electrode with dominant interictal epileptiform discharges (IEDs)...
July 24, 2017: Human Brain Mapping
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