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Takashi Sawamura, Tatsumi Tanaka, Hiroyuki Ishige, Masayuki Iizuka, Yasutoshi Murayama, Eigo Otsuji, Akihiro Ohkubo, Shun-Ichiro Ogura, Hideya Yuasa
An improvement in photodynamic therapy (PDT) efficiency against a human gastric cancer cell line (MKN45) with 5-aminolevulinic acid (ALA) and lanthanide nanoparticles (LNPs) is described. An endogenous photosensitizer, protoporphyrin IX, biosynthesized from ALA and selectively accumulated in cancer cells, is sensitizable by the visible lights emitted from up-conversion LNPs, which can be excited by a near-infrared light. Ten kinds of surface modifications were performed on LNPs, NaYF₄(Sc/Yb/Er) and NaYF₄(Yb/Tm), in an aim to distribute these irradiation light sources near cancer cells...
September 16, 2015: International Journal of Molecular Sciences
Justin R Ryder, Michael O'Connell, Tyler A Bosch, Lisa Chow, Kyle D Rudser, Donald R Dengel, Claudia K Fox, Julia Steinberger, Aaron S Kelly
BACKGROUND: We examined whether sympathetic nervous system activity influences hypertension status and systolic blood pressure (SBP) independent of adiposity in youth ranging from normal-weight to severe obesity. METHODS: We examined the association of heart rate variability (HRV) with hypertension status and SBP among youth (6-18 y old; n = 188; 103 female). Seated SBP was measured using an automated cuff. Prehypertension (SBP percentile ≥ 90th to <95th) and hypertension (SBP percentile ≥ 95th) were defined by age-, sex-, and height-norms...
January 2016: Pediatric Research
Anand Prasad, Carolina Ortiz-Lopez, David M Kaye, Melissa Byrne, Shane Nanayakkara, S Hinan Ahmed, Steven R Bailey, Roxana Mehran, Salil Sethi, Avantika Banerjee, Marvin Eng
BACKGROUND: The AVERT(TM) Contrast Modulation System (AVERT) (Osprey Medical, MN) is designed to reduce contrast volume administration during angiography. The AVERT provides an adjustable resistance circuit which decreases the pressure head delivering contrast towards the patient. The AVERT has not been previously studied in patients undergoing peripheral digital subtraction angiography (DSA). The purpose of this study was (1) to evaluate contrast savings with the AVERT and (2) to evaluate the ability to generate clinically acceptable DSA images in the process...
December 1, 2015: Catheterization and Cardiovascular Interventions
Kristine N Brazin, Robert J Mallis, Dibyendu Kumar Das, Yinnian Feng, Wonmuk Hwang, Jia-Huai Wang, Gerhard Wagner, Matthew J Lang, Ellis L Reinherz
The αβTCR was recently revealed to function as a mechanoreceptor. That is, it leverages mechanical energy generated during immune surveillance and at the immunological synapse to drive biochemical signaling following ligation by a specific foreign peptide-MHC complex (pMHC). Here, we review the structural features that optimize this transmembrane (TM) receptor for mechanotransduction. Specialized adaptations include (1) the CβFG loop region positioned between Vβ and Cβ domains that allosterically gates both dynamic T cell receptor (TCR)-pMHC bond formation and lifetime; (2) the rigid super β-sheet amalgams of heterodimeric CD3εγ and CD3εδ ectodomain components of the αβTCR complex; (3) the αβTCR subunit connecting peptides linking the extracellular and TM segments, particularly the oxidized CxxC motif in each CD3 heterodimeric subunit that facilitates force transfer through the TM segments and surrounding lipid, impacting cytoplasmic tail conformation; and (4) quaternary changes in the αβTCR complex that accompany pMHC ligation under load...
2015: Frontiers in Immunology
Yangwei Deng, Tao Zou, Xinfeng Tao, Vincent Semetey, Sylvain Trepout, Sergio Marco, Jun Ling, Min-Hui Li
Biocompatible amphiphilic block copolymers composed of polysarcosine (PSar) and poly(ε-caprolactone) (PCL) were synthesized using ring-opening polymerization of sarcosine N-thiocarboxyanhydride initiated by oxyamine-ended PCL and characterized by NMR, SEC, and DSC. Self-assembling of two triblock copolymers PSar8-b-PCL28-b-PSar8 (CS7) and PSar16-b-PCL40-b-PSar16 (CS10) in dilute solution was studied in detail toward polymersome formation using thin-film hydration and nanoprecipitation techniques. A few giant vesicles were obtained by thin-film hydration from both copolymers and visualized by confocal laser scanning microscope...
October 12, 2015: Biomacromolecules
Rym Zakhama-Sraieb, Yassine Ramzi Sghaier, Ahmed Ben Hmida, Giovanna Cappai, Alessandra Carucci, Faouzia Charfi-Cheikhrouha
The accumulation of the five trace metals (TMs) cadmium, copper, lead, nickel and zinc was measured in Posidonia oceanica leaves. Shoots were seasonally sampled at 8-10-m depth from four stations located in Port El Kantaoui area, Tunisia, during four campaigns performed in 2012. Levels of the five TMs were analyzed using inductively coupled plasma atomic emission spectrometry (ICP-AES) in three compartments of P. oceanica shoots: blades and sheaths of adult leaves and intermediate leaves. Results showed a preferential accumulation of Cd, Pb, Ni and Zn in adult leaf blades...
January 2016: Environmental Science and Pollution Research International
Cristiano Rossitto, Salvatore Gueli Alletti, Barbara Costantini, Francesco Fanfani, Giovanni Scambia
STUDY OBJECTIVE: To highlight the first case of laparoscopic hysterectomy performed with percutaneous instruments (The Percuvance System, Teleflex Incorporated, Wayne, PA). DESIGN: The basis of the system is a <3-mm diameter shaft that, when connected to an introducer tool tip, can be inserted percutaneously through the skin. The introducer tool tip can then be exchanged outside the body for a wide variety of 5-mm interchangeable surgical tool tips. The shaft is sufficiently strong to hold structures, and surgeons can use its interchangeable tool tips to grasp, cut, and manipulate tissue...
January 2016: Journal of Minimally Invasive Gynecology
Till F Jakob, Nicole Rosskothen-Kuhl, Robert-Benjamin Illing
Aim of this study was to induce a single-sided deafness (SSD) in rats before hearing onset. Rats were operated at postnatal day 10 by approaching the tympanic cavity along a retroauricular path without manipulating ossicles or tympanic membrane. The ototoxic aminoglycoside neomycin was injected intracochlearly through the round window membrane on one side. When the animals have reached young adult stages, their hearing threshold was determined by their auditory brainstem response (ABR). Monaural deafening was considered successful when the hearing threshold was at least 95 dB above the threshold of the normal hearing ear...
March 2016: Hearing Research
Simona Ferraro, Roberta Mozzi, Mauro Panteghini
OBJECTIVES: In this study, we evaluated the extent of inappropriate tumor marker (TM) ordering in a secondary care setting, approximately 6 years after the introduction of local guidelines, and we identified the main factors potentially influencing clinicians when performing an inappropriate TM request. METHODS: For this purpose, we regularly checked all requests containing more than two TMs. During the 21-month audit, the rate of rejected requests amounted to 3...
October 2015: American Journal of Clinical Pathology
Ridade Sayin, Laura Martinez-Marcos, Juan G Osorio, Paul Cruise, Ian Jones, Gavin W Halbert, Dimitrios A Lamprou, James D Litster
As twin screw granulation (TSG) provides one with many screw element options, characterization of each screw element is crucial in optimizing the screw configuration in order to obtain desired granule attributes. In this study, the performance of two different screw elements - distributive feed screws and kneading elements - was studied in an 11 mm TSG at different liquid-to-solid (L/S) ratios. The kneading element configuration was found to break large granules more efficiently, leading to narrower granule size distributions...
December 30, 2015: International Journal of Pharmaceutics
Katarzyna Trzmiel, Marzena Lewandowska
Soil-borne wheat mosaic virus (SBWMV) is a viral pathogen of cereal crops. Although first detected in the United States, it has since been reported in Germany and Poland. To date, two SBWMV strains (Nebraska-like and New-York-like types) have been detected. While the nucleotide sequences of the coat protein (CP) genes from the two strains show only 89% similarity, the deduced amino acid sequences of this region are identical. Therefore, the isolates cannot be differentiated serologically using the double antibody sandwich enzyme-linked immunosorbent assay...
December 1, 2015: Journal of Virological Methods
Jeong Jin Ahn, Ji-Hoon Kim, Youngjoo Kim, Ji Young Hong, Gi Won Kim, Seung Yong Hwang
Locked nucleic acid (LNA) is a modified RNA nucleotide that can be incorporated at specific positions to generate probes with the desired length, melting temperature (TM), and specificity. Here, we describe a method of multiplex genotyping based on dramatic shifts in the TM of a single dual-labeled LNA probe. Using this method, two varieties of the hairtail fish Trichiurus lepturus can be distinguished from each other, as well as from Trichiurus japonicus, based on a 1- to 2-bp difference in a fragment of mitochondrial cytochrome oxidase subunit 1...
December 15, 2015: Analytical Biochemistry
Irina V Botezatu, Irina O Nechaeva, Аnna М Stroganova, Anastasia I Senderovich, Valentina N Kondratova, Valery P Shelepov, Anatoly V Lichtenstein
The TaqMan probes that have been long and effectively used in real-time polymerase chain reaction (PCR) may also be used in DNA melting analysis. We studied some factors affecting efficiency of the approach such as (i) number of asymmetric PCR cycles preceding DNA melting analysis, (ii) choice of fluorophores for the multiplex DNA melting analysis, and (iii) choice of sense or antisense TaqMan probes for optimal resolution of wild-type and mutant alleles. We also determined ΔTm (i.e., the temperature shift of a heteroduplex relative to the corresponding homoduplex) as a means of preliminary identification of mutation type...
December 15, 2015: Analytical Biochemistry
Xia Xu, Zhuo Wang, Pengpeng Lei, Xiuling Liu, Yue Su, Lile Dong, Shuang Yao, Liang Zhou, Shuyan Song, Jing Feng, Hongjie Zhang
Cubic KLu3F10 nanocrystals have been fabricated by doping Mn(2+) ions via a liquid-solid solution (LSS) hydrothermal method. With an increase in the Mn(2+) level, a phase transformation from orthorhombic KLu2F7 to cubic KLu3F10 associated with evolution of the particle morphologies and sizes is evident. The phase transformation mechanism based on doping with Mn(2+) ions was studied via a series of time-controlled experiments and the enhancement of the intensity of upconversion luminescence (UCL) via doping with Mn(2+) ions is discussed in detail...
October 21, 2015: Dalton Transactions: An International Journal of Inorganic Chemistry
Ioannis G Koutelekos, Helen Kyritsi, Alexandros Makis, Constantine M Vassalos, Eftychios Ktenas, Maria Polikandrioti, Chryssa Tzoumaka-Bakoula, Nikolaos Chaliasos
Nowadays, thalassaemia major (TM) patients are surviving into mature young adulthood; however, no published instrument exists to measure the expectations' dimensionality among older TM patients in their thirties. This study seeks to validate a novel multidimensional expectation questionnaire suitable for TM patients (MEQ-TMP) reaching their fourth decade of life. In order to establish the psychometric properties of the instrument, data analysis was carried out. The principal component analysis revealed four components ('Supportive social network'; 'Raising one's own family'; 'Career advancement'; 'Ability of daily activities')...
2016: Global Journal of Health Science
Maya Al-Sid-Cheikh, Mathieu Pédrot, Martine Bouhnik-Le Coz, Aline Dia, Mélanie Davranche, Chrystelle Neaime, Fabien Grasset
The foundation of nanoscience is that the properties of materials change as a function of their physical dimensions, and nanotechnology exploits this premise by applying selected property modifications for a specific benefit. However, to investigate the fate and effect of the engineered nanoparticles on toxic metal (TM) mobility, the analytical limitations in a natural environment remain a critical problem to overcome. Recently, a new generation of size exclusion chromatography (SEC) columns developed with spherical silica is available for pore sizes between 5 and 400 nm, allowing the analysis of nanoparticles...
October 20, 2015: Analytical Chemistry
Yasin K Varsak, Peter L Santa Maria
CONCLUSION: The present study demonstrates a mouse model of chronic Eustachian tube (ET) dysfunction using a surgical technique that is reproducible and effective with low mortality and morbidity. OBJECTIVE: To create a reproducible and effective mouse model of ET obstruction with a low operative morbidity. METHODS: Forty healthy, male CBA/CaJ mice underwent the procedure. ET obstruction was performed on one side under general anesthesia; A small piece of dental material (Gutta Percha Points, Meta Biomed, Chungbuk, Korea) was placed via the tympanic orifice of the ET to occlude the entire ET...
2016: Acta Oto-laryngologica
Zahra Polat, Burcu Baş, Duygu Hayır, Erdoğan Bulut, Ahmet Ataş
OBJECTIVE: The aim of this study was to obtain norm values for a young adult Turkish group and to investigate the differences between female and male subjects in terms of wideband tympanometry. MATERIALS AND METHODS: One hundred ten young adult volunteers (mean±SD: 21.1±1.9 years) participated in this study. The measurements of wideband tympanometry were performed at octave frequencies between 226 Hz and 8000 Hz using Titan version 3.1. The stimulus level was set at 100 dB peSPL...
August 2015: Journal of International Advanced Otology
Abdullah Dalgıç, Oğuz Yılmaz, Yusuf Hıdır, Bülent Satar, Mustafa Gerek
OBJECTIVE: Our objective was to analyze the electrocochleography (ECoG) and cervical vestibular evoked myogenic potential (cVEMP) results of patients with noise-induced hearing loss (NIHL). MATERIALS AND METHODS: The study included 20 patients with NIHL. Pure-tone audiometry, tympanic membrane ECoG, and cVEMP were performed on all patients. The patients were divided into two groups based on averaged thresholds at 4, 6, and 8 kHz; whereby, group 1 comprised patients who had a threshold higher than 68...
August 2015: Journal of International Advanced Otology
Junqing Wang, Hak Soo Choi, Yì-Xiáng J Wáng
Publication statistics was retrieved on carbon nanodots (C-dots) from 2004 up till 2014 using the web of Science(TM) search engine. The number of publications from Chinese authors increased exponentially during this period. Till 2014 China mainland authors contributed 47% of the total publications. Publications on pharmacology and toxicology lagged far behind the publications on chemistry and material science, indicating that research is not solidly moving toward the direction of application.
July 2015: Journal of Thoracic Disease
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