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https://www.readbyqxmd.com/read/28330285/screening-production-optimization-and-characterization-of-%C3%AE-glucosidase-using-microbes-from-shellfish-waste
#1
Samhita Mahapatra, A S Vickram, T B Sridharan, R Parameswari, M Ramesh Pathy
An extracellular β-glucosidase was isolated from Proteus mirabilis VIT117 found to be growing on prawn shells. The enzyme production was found to be enhanced (14.58 U/ml) when the culture was maintained at pH 9 and provided with sorbitol as carbon source, yeast extract as nitrogen source and incubated at 37 °C for approximately 72 h. Statistical methods like Plackett-Burman and RSM were also applied here to study the effects of different combinations of growth parameters for the bacteria, where the most significant parameters were found to be inoculum size, pH, yeast extract, incubation time and sorbitol...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330263/multi-substrate-biodegradation-of-chlorophenols-by-defined-microbial-consortium
#2
Bhishma P Patel, Arvind Kumar
In the present study, a defined mixed microbial consortium was investigated for their ability to utilize three different monochlorophenols (MCPs) and 2,4-DCP individually and in the mixture. None of the individual strains were able to utilize 3-CP and 4-CP, but when they were mixed to form defined consortium, they have shown great potential and degradation of high concentration of 3-CP and 4-CP. Spectrophotometric analysis of metabolites during MCPs degradation establishes the presence of 2-chloromaleylacetate...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330251/stenotrophomonas-sp-rzs-7-a-novel-phb-degrader-isolated-from-plastic-contaminated-soil-in-shahada-maharashtra-western-india
#3
S J Wani, S S Shaikh, B Tabassum, R Thakur, A Gulati, R Z Sayyed
This paper reports an isolation and identification of novel poly-β-hydroxybutyrate (PHB) degrading bacterium Stenotrophomonas sp. RZS 7 and studies on its extracellular PHB degrading depolymerase enzyme. The bacterium isolated from soil samples of plastic contaminated sites of municipal area in Shahada, Maharashtra, Western India. It was identified as Stenotrophomonas sp. RZS 7 based on polyphasic approach. The bacterium grew well in minimal salt medium (MSM) and produced a zone (4.2 mm) of PHB hydrolysis on MSM containing PHB as the only source of nutrient...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330236/xylanase-production-from-penicillium-citrinum-isolate-hzn13-using-response-surface-methodology-and-characterization-of-immobilized-xylanase-on-glutaraldehyde-activated-calcium-alginate-beads
#4
Zabin K Bagewadi, Sikandar I Mulla, Yogesh Shouche, Harichandra Z Ninnekar
The present study reports the production of high-level cellulase-free xylanase from Penicillium citrinum isolate HZN13. The variability in xylanase titers was assessed under both solid-state (SSF) and submerged (SmF) fermentation. SSF was initially optimized with different agro-waste residues, among them sweet sorghum bagasse was found to be the best substrate that favored maximum xylanase production (9643 U/g). Plackett-Burman and response surface methodology employing central composite design were used to optimize the process parameters for the production of xylanase under SSF...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330233/improvement-of-shelf-life-of-soymilk-using-immobilized-protease-of-oerskovia-xanthineolytica-ncim-2839
#5
A K Sahoo, V S Gaikwad, R C Ranveer, P B Dandge, S R Waghmare
Protease enzyme has lot of commercial applications, so the cost-effective production of protease using sunflower oil seed waste was carried out from Oerskovia xanthineolyitca NCIM 2839. The maximum protease production was after 24 h of incubation with 2.5 % oil seed waste concentration. O. xanthineolytica was found to produce two proteases-P1 and P2. The proteases were purified using 60 % cold acetone precipitation and DEAE-cellulose ion exchange chromatography. SDS-PAGE revealed molecular weight of P1 and P2 was 36 and 24 kDa, respectively...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330226/anti-oxidant-and-anti-microbial-properties-of-some-ethno-therapeutically-important-medicinal-plants-of-indian-himalayan-region
#6
Sandeep Rawat, Arun K Jugran, Amit Bahukhandi, Asutosh Bahuguna, Indra D Bhatt, Ranbeer S Rawal, Uppeandra Dhar
Therapeutic potential of medicinal plants as a source of noble natural anti-oxidants and anti-microbial agents has been well recognised all across the globe. In this study, phenolic compounds, in vitro anti-oxidant activity and anti-microbial properties have been investigated in five Himalayan medicinal plants, (e.g., Acorus calamus, Habenaria intermedia, Hedychium spicatum, Roscoea procera and Valeriana jatamansi) in different solvent systems. R. procera exhibited significantly (p < 0.05) higher phenolics; while H...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330153/an-evaluation-of-the-major-factors-influencing-the-removal-of-copper-ions-using-the-egg-shell-dromaius-novaehollandiae-chitosan-agaricus-bisporus-composite
#7
R K Anantha, S Kota
Rapid industrialisation, technological development, urbanization and increase in population in the recent past coupled with unplanned and unscientific disposal methods led to increased heavy metal levels in water. Realizing the need for development of eco-friendly and cost effective methods, the present investigation was done for the adsorptive removal of copper from aqueous solutions with Dromaius novaehollandiae eggshell and chitosan composite. By one variable at a time method, the optimum contact time was found to be 60 min with an adsorbent dosage of 8 g/L at pH 6, initial adsorbate concentration of 20 mg/L and temperature 30 °C...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330140/statistical-based-optimization-and-scale-up-of-siderophore-production-process-on-laboratory-bioreactor
#8
S S Shaikh, S J Wani, R Z Sayyed
We report the enhanced production of siderophore in succinate medium by applying two-stage statistical approach, i.e., Plackett-Burman design and response surface methodology (RSM) using central composite design (CCD). In the first stage of optimization, out of 11 variable components of succinate medium, succinic acid, pH and temperature were found as significant components that influenced the siderophore production in Pseudomonas aeruginosa RZS9. The second stage of RSM using CCD consisted of optimizing the concentrations of the variables...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330135/confamiliar-transferability-of-simple-sequence-repeat-ssr-markers-from-cotton-gossypium-hirsutum-l-and-jute-corchorus-olitorius-l-to-twenty-two-malvaceous-species
#9
Pratik Satya, Pramod Kumar Paswan, Swagata Ghosh, Snehalata Majumdar, Nasim Ali
Cross-species transferability is a quick and economic method to enrich SSR database, particularly for minor crops where little genomic information is available. However, transferability of SSR markers varies greatly between species, genera and families of plant species. We assessed confamiliar transferability of SSR markers from cotton (Gossypium hirsutum) and jute (Corchorus olitorius) to 22 species distributed in different taxonomic groups of Malvaceae. All the species selected were potential industrial crop species having little or no genomic resources or SSR database...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330132/an-efficient-and-cost-effective-method-for-dna-extraction-from-athalassohaline-soil-using-a-newly-formulated-cell-extraction-buffer
#10
Avinash Narayan, Kunal Jain, Amita R Shah, Datta Madamwar
The present study describes the rapid and efficient indirect lysis method for environmental DNA extraction from athalassohaline soil by newly formulated cell extraction buffer. The available methods are mostly based on direct lysis which leads to DNA shearing and co-extraction of extra cellular DNA that influences the community and functional analysis. Moreover, during extraction of DNA by direct lysis from athalassohaline soil, it was observed that, upon addition of poly ethylene glycol (PEG), isopropanol or absolute ethanol for precipitation of DNA, salt precipitates out and affecting DNA yield significantly...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330121/optimization-study-of-2-hydroxyquinoxaline-2-hq-biodegradation-by-ochrobactrum-sp-hq1
#11
G V Subba Reddy, M Md Rafi, S Rubesh Kumar, N Khayalethu, D Muralidhara Rao, B Manjunatha, G H Philip, B R Reddy
A novel aerobic gram-negative bacterial strain capable of utilizing 2-hydroxyquinoxaline (2-HQ) as sole source of carbon and energy was isolated from Indian agricultural soil and named as HQ1. Strain HQ1 was identified as Ochrobactrum sp. on the basis of morphology, physico-biochemical characteristics and 16S rRNA sequence analysis. The generation time of Ochrobactrum sp. HQ1 on 2-HQ at log phase is 0.71 h or 42.6 min. The degradation of 2-HQ by HQ1 under various physico-chemical parameters was analysed by HPLC and observed to be optimum with a high inoculum density (1...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330119/degradation-of-fipronil-by-stenotrophomonas-acidaminiphila-isolated-from-rhizospheric-soil-of-zea-mays
#12
Shivani Uniyal, Rashmi Paliwal, R K Sharma, J P N Rai
Fipronil is a widely used insecticide in agriculture and can cause potential health hazards to non-target soil invertebrates and nearby aquatic systems. In the present study, a fipronil degrading bacterium was isolated from fipronil contaminated soil, i.e. rhizospheric zone of Zea mays. Morphological, biochemical and molecular characterization of strain indicated that it clearly belongs to Stenotrophomonas acidaminiphila (accession no. KJ396942). A three-factor Box-Behnken experimental design combined with response surface modeling was employed to predict the optimum conditions for fipronil degradation...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330091/optimization-of-regeneration-using-differential-growth-regulators-in-indica-rice-cultivars
#13
Samar Shekar R Sankepally, Bharat Singh
Paddy is a staple crop and being grown in major parts of India, but similarly it is facing some major challenges like abiotic stresses (drought, salinity, etc.) by which its productivity is greatly affected. De-husked seeds of 21 cultivars of indica rice were cultured onto MS medium for screening of their micropropagation capabilities. All the selected cultivars are being cultivated by farmers of Andhra Pradesh and Rajasthan states of India, these cultivars bear good quality of grain and high yield. Similarly, both states are facing the problem of drought so; we selected these cultivars for the micropropagation and transformation studies...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330080/transgenic-rice-plants-expressing-synthetic-cry2ax1-gene-exhibits-resistance-to-rice-leaffolder-cnaphalocrosis-medinalis
#14
R Manikandan, N Balakrishnan, D Sudhakar, V Udayasuriyan
Bacillus thuringiensis is a major source of insecticidal genes imparting insect resistance in transgenic plants. Level of expression of transgenes in transgenic plants is important to achieve desirable level of resistance against target insects. In order to achieve desirable level of expression, rice chloroplast transit peptide sequence was fused with synthetic cry2AX1 gene to target its protein in chloroplasts. Sixteen PCR positive lines of rice were generated by Agrobacterium mediated transformation using immature embryos...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330079/evaluation-of-photosynthetic-efficacy-and-co2-removal-of-microalgae-grown-in-an-enriched-bicarbonate-medium
#15
S Abinandan, S Shanthakumar
Bicarbonate species in the aqueous phase is the primary source for CO2 for the growth of microalgae. The potential of carbon dioxide (CO2) fixation by Chlorella pyrenoidosa in enriched bicarbonate medium was evaluated. In the present study, effects of parameters such as pH, sodium bicarbonate concentration and inoculum size were assessed for the removal of CO2 by C. pyrenoidosa under mixotrophic condition. Central composite design tool from response surface methodology was used to validate statistical methods in order to study the influence of these parameters...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28329935/-a-cross-sectional-survey-of-receiving-no-methadone-maintenance-treatment-in-hiv-infected-injecting-drug-users-in-dehong-dai-and-jingpo-autonomous-prefecture-yunnan-province
#16
R H Tang, R H Ye, Y C Yang, S T Yao, J B Wang, R M Zhang, L F Luo, Z L Wu, Y C Long, M S Yin, N He, S Duan
Objective: To understand the current status of receiving no methadone maintenance treatment (MMT) and influencing factors in HIV infected injecting drug users (IDUs) in Dehong Dai and Jingpo autonomous prefectures, Yunnan province. Methods: Data of survival of IDUs with AIDS in Dehong were collected from " Chinese National Comprehensive HIV/AIDS and Care Information System" in December, 2014. Results: There were 987 IDUs who should receive MMT, the majority of them were males (94.6%, 934/987), aged 35-44 years (53...
March 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://www.readbyqxmd.com/read/28329930/-commuting-mode-specific-exposure-to-pm-2-5-in-urban-area-of-guangzhou
#17
X J Lyu, Z H Li, X Li, W L Zeng, P Yang, Q X Lin, J Y Zheng, X L Du, Y Z Gu, Y Q Zhao, R S Xie, T Liu, H L Lin, W J Ma
Objective: To find the differences in PM(2.5) exposure level in the context of four commuting modes (by walk, bicycle, bus and subway) in Guangzhou. Methods: The PM(2.5) exposure assessment was carried out from January to December 2015 in Guangzhou. PM(2.5) was measured by using SidePak individual dust meter (AM510, TSI Inc. USA) with time interval of 1 minute. Our measurement was taken on Monday, Wednesday, Friday and Sunday in the second week of each month and the samples were collected in the morning (07:00-09:00), afternoon (11:00-13:00) and evening (17:00-19:00)...
March 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://www.readbyqxmd.com/read/28329926/-acute-effect-of-fine-particulate-matters-on-daily-cardiovascular-disease-mortality-in-seven-cities-of-china
#18
R M Liang, P Yin, L J Wang, Y C Li, J M Liu, Y N Liu, J L You, J L Qi, M G Zhou
Objective: To explore the effect of fine particulate matters with an aerodynamic diameter less than 2.5 μm (PM(2.5)) on daily cardiovascular disease mortality in seven cities of China. Methods: Daily average concentrations of PM(2.5), cardiovascular disease mortality data and environmental data were collected from January 1, 2013 to December 31, 2015 in seven cities of China, including Shijiazhuang, Haerbin, Shanghai, Wuhan, Guangzhou, Chengdu and Xi' an. We linked generalized additive model with Quasi-Poisson distribution to evaluate the association between daily concentrations of PM(2...
March 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://www.readbyqxmd.com/read/28329845/spatiotemporal-progression-and-remission-of-lesions-in-the-rat-brain-following-acute-intoxication-with-diisopropylfluorophosphate
#19
Sílvia Sisó, Brad A Hobson, Danielle J Harvey, Donald A Bruun, Douglas J Rowland, Joel R Garbow, Pamela J Lein
Similar to organophosphate (OP) nerve agents, diisopropylfluorophosphate (DFP) rapidly and irreversibly inhibits acetylcholinesterase, leading to convulsions that can progress to status epilepticus (SE). However, in contrast to the OP nerve agents, the long-term consequences of DFP-induced SE are not well known. Thus, we characterized the spatiotemporal profile of neuropathology during the first two months following acute DFP intoxication. Adult, male Sprague Dawley rats administered pyridostigmine bromide (0...
March 7, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28329842/magnetic-resonance-imaging-reveals-progressive-brain-injury-in-rats-acutely-intoxicated-with-diisopropylfluorophosphate
#20
Brad A Hobson, Sílvia Sisó, Douglas J Rowland, Danielle J Harvey, Donald A Bruun, Joel R Garbow, Pamela J Lein
Acute intoxication with organophosphates (OPs) can trigger seizures that progress to status epilepticus, and survivors often exhibit chronic neuropathology, cognitive impairment, affective disorders and/or electroencephalographic abnormalities. Understanding how acute injury transitions to persistent neurological sequelae is critical to developing medical countermeasures for mitigating damage following OP-induced seizures. Here, we used in vivo magnetic resonance imaging (MRI) to monitor the spatiotemporal patterns of neuropathology for one month after acute intoxication with diisopropylfluorophosphate (DFP)...
March 7, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
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